Tan0020275 (gene) Snake gourd v1

Overview
NameTan0020275
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionF-box protein
LocationLG07: 4502473 .. 4503701 (-)
RNA-Seq ExpressionTan0020275
SyntenyTan0020275
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCCGCTGCAAAGCTCCTAGAATCGTCATCCGGTTTTCCTCCTCCGTCGCCGCCAAATCAGTCGCCGATAACGACGATCTCTTACTCCAGATTCTTCTCCGTTTACCCATCCGATCACTTCTACGATTCAAATCCGTCTCAAAACGATGGCTCTCCCTAATTTCTGATCCCAATTTCTCCTACCGTCGAATCACTTCTCATCCGCCCATTCCTTCAGGTATCTTCTTGTCCCGTCCCCACCCTATTTCCCCTGAATTCGATTTCGTCGATTTGCGCTCAAATCCGCCTAGTGTTCCGTTCGAATCTCTGCAATTCTCTGTGCCGAAACATGGAATTGTAATTTTGCAATCCTGTAACGGCTTGCTGTTGTGCTGCAACGAAGGTCAAACCTACCCGAAGACCGATTATTACGTTTACAATCCAACGACGAAGCAATGCACAACGCTTCAGATTGACAGAACCAGAGAAATCGTTGGTGTTAATTTGGCATTCGATCCTTTGCGATCATCAAATTACAAAGTCGTTTGCGTTCGAATTCTTGATTATTCTAACAACTACCAAATCGAGATTTACTCGCCTGATTCTGGCTTCTGGAGGCCCTGCAATGGCCGATTCTCTCCCCCATACAACACAAAATTCGATTACGGAGTTTACTGGAATGGAGCTGTTCATTGGATAAGGAATTGGGGAAACTGCGTTTACTTGGATTTGTGCGAAGAGAAACTCCATGAATTGCCAATACCTCGAGGTTCTGATGGATGGGAAGGTAGAACAATGGAGTATTTTGGAAATTGCTGTGGGCGTCTTCATTGGATTGAAAGTTCTGGACGGCAATCTGTGTTGAATGTGTACGAGATGGAGTATGATCGTTGTGGGTGGTTTGAGAAGTATCGGATTGATCTTCATACGGTATCTGGGTTGTTCCCAAAAATGAGTCCGGCTCCAGAGGAGTCGTTGAATTTGTGCAAGTTCACGGTGCTCGCCATTGTTGATCAGAGTAAGGTGGATGAAGATCCATATGTTATTTTGCAGGGTCCTAGAAAATTAGCTGTACGATTGAATGTGAAGACTGGGAGCTTTGAGAGGTTAGTGTACGTTCTAAATGATGGAGGAACCACAAAATTTCCCTTAGGGTTTGGAGTGATTGAACCATTTTTGTACATTGAGAGTCTTGCATGTGTTTAAGGATAAATTATTCGACGTTCCCGTCACAGCTTCTTAAATGA

mRNA sequence

ATGCTCCGCTGCAAAGCTCCTAGAATCGTCATCCGGTTTTCCTCCTCCGTCGCCGCCAAATCAGTCGCCGATAACGACGATCTCTTACTCCAGATTCTTCTCCGTTTACCCATCCGATCACTTCTACGATTCAAATCCGTCTCAAAACGATGGCTCTCCCTAATTTCTGATCCCAATTTCTCCTACCGTCGAATCACTTCTCATCCGCCCATTCCTTCAGGTATCTTCTTGTCCCGTCCCCACCCTATTTCCCCTGAATTCGATTTCGTCGATTTGCGCTCAAATCCGCCTAGTGTTCCGTTCGAATCTCTGCAATTCTCTGTGCCGAAACATGGAATTGTAATTTTGCAATCCTGTAACGGCTTGCTGTTGTGCTGCAACGAAGGTCAAACCTACCCGAAGACCGATTATTACGTTTACAATCCAACGACGAAGCAATGCACAACGCTTCAGATTGACAGAACCAGAGAAATCGTTGGTGTTAATTTGGCATTCGATCCTTTGCGATCATCAAATTACAAAGTCGTTTGCGTTCGAATTCTTGATTATTCTAACAACTACCAAATCGAGATTTACTCGCCTGATTCTGGCTTCTGGAGGCCCTGCAATGGCCGATTCTCTCCCCCATACAACACAAAATTCGATTACGGAGTTTACTGGAATGGAGCTGTTCATTGGATAAGGAATTGGGGAAACTGCGTTTACTTGGATTTGTGCGAAGAGAAACTCCATGAATTGCCAATACCTCGAGGTTCTGATGGATGGGAAGGTAGAACAATGGAGTATTTTGGAAATTGCTGTGGGCGTCTTCATTGGATTGAAAGTTCTGGACGGCAATCTGTGTTGAATGTGTACGAGATGGAGTATGATCGTTGTGGGTGGTTTGAGAAGTATCGGATTGATCTTCATACGGTATCTGGGTTGTTCCCAAAAATGAGTCCGGCTCCAGAGGAGTCGTTGAATTTGTGCAAGTTCACGGTGCTCGCCATTGTTGATCAGAGTAAGGTGGATGAAGATCCATATGTTATTTTGCAGGGTCCTAGAAAATTAGCTGTACGATTGAATGTGAAGACTGGGAGCTTTGAGAGGATAAATTATTCGACGTTCCCGTCACAGCTTCTTAAATGA

Coding sequence (CDS)

ATGCTCCGCTGCAAAGCTCCTAGAATCGTCATCCGGTTTTCCTCCTCCGTCGCCGCCAAATCAGTCGCCGATAACGACGATCTCTTACTCCAGATTCTTCTCCGTTTACCCATCCGATCACTTCTACGATTCAAATCCGTCTCAAAACGATGGCTCTCCCTAATTTCTGATCCCAATTTCTCCTACCGTCGAATCACTTCTCATCCGCCCATTCCTTCAGGTATCTTCTTGTCCCGTCCCCACCCTATTTCCCCTGAATTCGATTTCGTCGATTTGCGCTCAAATCCGCCTAGTGTTCCGTTCGAATCTCTGCAATTCTCTGTGCCGAAACATGGAATTGTAATTTTGCAATCCTGTAACGGCTTGCTGTTGTGCTGCAACGAAGGTCAAACCTACCCGAAGACCGATTATTACGTTTACAATCCAACGACGAAGCAATGCACAACGCTTCAGATTGACAGAACCAGAGAAATCGTTGGTGTTAATTTGGCATTCGATCCTTTGCGATCATCAAATTACAAAGTCGTTTGCGTTCGAATTCTTGATTATTCTAACAACTACCAAATCGAGATTTACTCGCCTGATTCTGGCTTCTGGAGGCCCTGCAATGGCCGATTCTCTCCCCCATACAACACAAAATTCGATTACGGAGTTTACTGGAATGGAGCTGTTCATTGGATAAGGAATTGGGGAAACTGCGTTTACTTGGATTTGTGCGAAGAGAAACTCCATGAATTGCCAATACCTCGAGGTTCTGATGGATGGGAAGGTAGAACAATGGAGTATTTTGGAAATTGCTGTGGGCGTCTTCATTGGATTGAAAGTTCTGGACGGCAATCTGTGTTGAATGTGTACGAGATGGAGTATGATCGTTGTGGGTGGTTTGAGAAGTATCGGATTGATCTTCATACGGTATCTGGGTTGTTCCCAAAAATGAGTCCGGCTCCAGAGGAGTCGTTGAATTTGTGCAAGTTCACGGTGCTCGCCATTGTTGATCAGAGTAAGGTGGATGAAGATCCATATGTTATTTTGCAGGGTCCTAGAAAATTAGCTGTACGATTGAATGTGAAGACTGGGAGCTTTGAGAGGATAAATTATTCGACGTTCCCGTCACAGCTTCTTAAATGA

Protein sequence

MLRCKAPRIVIRFSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNFSYRRITSHPPIPSGIFLSRPHPISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVILQSCNGLLLCCNEGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVVCVRILDYSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVYLDLCEEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIESSGRQSVLNVYEMEYDRCGWFEKYRIDLHTVSGLFPKMSPAPEESLNLCKFTVLAIVDQSKVDEDPYVILQGPRKLAVRLNVKTGSFERINYSTFPSQLLK
Homology
BLAST of Tan0020275 vs. ExPASy Swiss-Prot
Match: Q9FLS0 (F-box protein At5g07610 OS=Arabidopsis thaliana OX=3702 GN=At5g07610 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.9e-53
Identity = 150/391 (38.36%), Postives = 214/391 (54.73%), Query Frame = 0

Query: 3   RCKAPRIVIR-----FSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISD 62
           R KAPR         FSSS +A  VAD DD+L+QIL  LPI++LLRFK VSKRWLSLI++
Sbjct: 8   RTKAPRSARSRRNGGFSSS-SATIVADIDDVLIQILSFLPIKTLLRFKRVSKRWLSLITN 67

Query: 63  PNFSYRRITSHPPIP-SGIFLSRPHPISPEFDFVDLRSNPPSVPFES---LQFSVPKHGI 122
           P FS R I S+ P+P SG FL  P  I  ++ FV L  +  +    S   L F+  +  +
Sbjct: 68  PVFSNRVIKSNHPLPISGFFLHSPREI--KYSFVSLDDDATNQRISSSLPLWFTDHQTDM 127

Query: 123 VILQSCNGLLLC-CN-EGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSS 182
           +I+QS NGLLLC C+     +  T+YYVYNPTTKQ T L   +    + ++LAFDP RS 
Sbjct: 128 IIMQSTNGLLLCKCSCASSNHFNTNYYVYNPTTKQYTLLH--QIAGHIALSLAFDPSRSP 187

Query: 183 NYKVVCVRILDYSNN----------YQIEIYSPDSGFW-RPCNGRFSPPYNTKFDYGVYW 242
           +YKV C+R    SNN          Y IE+YS + G W R      SP    +F Y V+W
Sbjct: 188 HYKVFCLR--GRSNNSFSSASDSELYHIEVYSSNEGLWRRVVPVPTSPSTFIEFSYSVFW 247

Query: 243 NGAVHWIR-NWGNCVYLDLCEEKLHELPIPRGSDGWE----GRTMEYFGNCCGRLHWIES 302
           NGAV+W   +  +C+  D+  +++  LP+P      E     R + +     G L++IE 
Sbjct: 248 NGAVNWYGFSSRDCLSFDINTQEIKILPLPDHEHEDEPLPDPRILMFLDESQGNLYYIEV 307

Query: 303 SGRQSV-LNVYEMEYDRCGWFEKYRIDLHTVSGLFPKMSPAP-EESLNLCKFTVLAIVDQ 362
           + + S  L VYEME +   W  KY +DL  ++  FP+M          +  F+V+  V +
Sbjct: 308 NNQSSSNLRVYEMESNSSSWSVKYNVDLEPLAAAFPEMIRTEYYTDRRIYAFSVIGFV-K 367

Query: 363 SKVDEDPYVILQGPRKLAVRLNVKTGSFERI 365
            + D   Y++L  P + AV+ N    +F+++
Sbjct: 368 EETDAASYILLHIPNQ-AVKYNFIDKTFKKL 389

BLAST of Tan0020275 vs. ExPASy Swiss-Prot
Match: Q9LPW2 (Putative F-box/kelch-repeat protein At1g12870 OS=Arabidopsis thaliana OX=3702 GN=At1g12870 PE=4 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 4.2e-12
Identity = 72/253 (28.46%), Postives = 117/253 (46.25%), Query Frame = 0

Query: 26  DDLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNFSYRRI----TSHPPIPSGIFLSRPH 85
           DD++ +I L+LP+++L+RFKS+SK+W S +    FS R +     SH   P  + ++   
Sbjct: 35  DDVVEEIFLKLPVKALMRFKSLSKQWRSTLESCYFSQRHLKIAERSHVDHPKVMIITEKW 94

Query: 86  PISPEFDFVDLRSNPPSVPF-ESLQFSVPK---HGIVILQSCNGLLLCCNEGQTYPKT-D 145
             +P+ +      +  SV F  S  F+ P+   H I   +SC+G + C +     PKT D
Sbjct: 95  --NPDIEISFRTISLESVSFLSSALFNFPRGFHHPIYASESCDG-IFCIHS----PKTQD 154

Query: 146 YYVYNPTTKQCTTL--------------QIDRTREIVGVN-LAFDPLRSSNYKVVCVRIL 205
            YV NP T+    L               +D  R+++ VN LAF  +++++YK+V +   
Sbjct: 155 IYVVNPATRWFRQLPPARFQIFMHKLNPTLDTLRDMIPVNHLAF--VKATDYKLVWLYNS 214

Query: 206 DYSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDY-GVYWNGAVHWIRNWGNC----VYL 250
           D S   + E++   +  WR       P Y    D      NG ++W     N     + L
Sbjct: 215 DASRVTKCEVFDFKANAWRYLT--CIPSYRIYHDQKPASANGTLYWFTETYNAEIKVIAL 274

BLAST of Tan0020275 vs. ExPASy Swiss-Prot
Match: Q9LFW0 (Putative F-box protein At5g15660 OS=Arabidopsis thaliana OX=3702 GN=At5g15660 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.0e-10
Identity = 64/220 (29.09%), Postives = 100/220 (45.45%), Query Frame = 0

Query: 27  DLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNF--SYRRITSHPP---IPSGIFLSRPH 86
           DL+++IL RLP++S+ RF +VSK W + I  P+F  SYR  +S  P   I S +    P+
Sbjct: 31  DLVIEILERLPLKSVARFLTVSKLWATTIRSPDFRKSYRGGSSSEPRTLIVSDLNFKEPN 90

Query: 87  PISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVILQSCNGLLLCCNEGQTYPKTDYYVYN 146
           P      F    S+P  +   +  F+ P+H        NGL+           TD  V N
Sbjct: 91  P--KLHFFRPSISSPSFLSSLTCPFTYPRHEEYYYHHVNGLI------SVGYGTDQIVIN 150

Query: 147 PTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVVCV--RILDY----SNNYQIEIYSPD 206
           PTT +  TL   +TR  + ++       S  YKV+C+  R+  +    S+ +Q+      
Sbjct: 151 PTTGKFITLPRPKTRRKLVISFFGYDSVSDQYKVLCMTERLRGHPEEASSQHQVYTLGAK 210

Query: 207 SGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVY 236
              W+  N   S P+       V  NG V++I   G  ++
Sbjct: 211 QKSWKMIN--CSIPHRPWSWNAVCINGVVYYIAKTGEGMF 240

BLAST of Tan0020275 vs. ExPASy Swiss-Prot
Match: Q9S9V1 (Putative F-box only protein 15 OS=Arabidopsis thaliana OX=3702 GN=FBX15 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 7.4e-09
Identity = 55/205 (26.83%), Postives = 87/205 (42.44%), Query Frame = 0

Query: 27  DLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNFSYRRITSHPPIPSGIFLSRPHPISPE 86
           +L+ +IL + P  SL RFKS  K+W  +I+   F Y  +   P     I   +   I   
Sbjct: 14  ELVEEILKKTPAESLNRFKSTCKQWYGIITSKRFMYNHLDHSPERFIRIDDHKTVQIMDP 73

Query: 87  FDFVDLRSNPPSVPFESLQFSVPKHGIVILQSCNGLLLCCNEGQTYPKT---DYYVYNPT 146
              +   S  P V      F  P H    +  C+GL+LC     +Y +T   +  V+NP 
Sbjct: 74  MTGIFSDSPVPDV------FRSP-HSFASMVHCDGLMLCICSDSSYERTREANLAVWNPV 133

Query: 147 TKQCTTLQ-IDRTREIVGVNLAFDPLRSSNYKVVCVRILDYSNNYQIEIYSPDSGFWRPC 206
           TK+   ++ +D   E     + +D     NYK+V        ++ + EIY   S  WR  
Sbjct: 134 TKKIKWIEPLDSYYETDYFGIGYDNTCRENYKIVRFSGPMSFDDTECEIYEFKSDSWRTL 193

Query: 207 NGRFSPPYNTKFDYGVYWNGAVHWI 228
           + ++   Y      GV   G ++WI
Sbjct: 194 DTKYWDVYTQ--CRGVSVKGNMYWI 209

BLAST of Tan0020275 vs. ExPASy Swiss-Prot
Match: Q3E9Z8 (Putative F-box protein At4g17780 OS=Arabidopsis thaliana OX=3702 GN=At4g17780 PE=4 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 9.7e-09
Identity = 82/306 (26.80%), Postives = 128/306 (41.83%), Query Frame = 0

Query: 27  DLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNFSYRRITSHPPIPSGIFLSRPHPISPE 86
           DLL  I LRLP++S+L  KSVSKRW S++    F  RR++          L +   I   
Sbjct: 16  DLLEDIFLRLPLKSILISKSVSKRWRSILESKTFVERRMS----------LQKKRKILAA 75

Query: 87  FDFV---DLRSNPPSVPFESLQFSVPKHGIVILQS--CNGLLLCCNEGQTYPKTDYYVYN 146
           ++     + R  P S   +  +  V  H      S  C+G L+C  E +        V N
Sbjct: 76  YNCKCGWEPRLLPGSSQCKGNEEIVYLHCNAAQPSFTCDG-LVCILEPRWID-----VLN 135

Query: 147 PTTKQCTTLQIDRTREIVGVNLAFDPLR----------SSNYKVVCVRILDYSN------ 206
           P T+Q           I GV  AF P            + +YKVV + ++ +S       
Sbjct: 136 PWTRQLRRYGFG-FGTIFGVGSAFSPRHWAMGFGKDKVTGSYKVVKMCLISFSEICARDP 195

Query: 207 NYQIEIYSPDSGFWRPCNGRFSPPYNT-KFDYGVYWNGAVHWI-----RNWGNCVYLDLC 266
             +  +   ++G WR  +    PPY   +       NG+++W+     R W   + LDL 
Sbjct: 196 EVEYSVLDVETGEWRMLS---PPPYKVFEVRKSECANGSIYWLHKPTERAW-TILALDLH 255

Query: 267 EEKLHELPIPRGSDGWEGRTMEYFG--NCCGRLHWIES-SGRQSVLNVYEMEYDRCGWFE 303
           +E+LH + +P  S      T E F   N   RL    + +  +  L ++ M+ +   W +
Sbjct: 256 KEELHNISVPDMS-----VTQETFQIVNLEDRLAIANTYTKTEWKLEIWSMDTEVETWTK 295

BLAST of Tan0020275 vs. NCBI nr
Match: XP_022929085.1 (F-box protein At5g07610 [Cucurbita moschata])

HSP 1 Score: 428.3 bits (1100), Expect = 6.7e-116
Identity = 218/368 (59.24%), Postives = 271/368 (73.64%), Query Frame = 0

Query: 1   MLRCKAPRIVIRFSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNF 60
           ML  K+P+  I  +S+ A + V +NDDL L+IL+RLPIRSLL FKSVSKRWL+LIS+P+F
Sbjct: 1   MLCSKSPK--IHRTSTAAVEPVLNNDDLFLEILIRLPIRSLLTFKSVSKRWLALISNPSF 60

Query: 61  SYRRITSHPPIPSGIFLSRPHPISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVILQSCN 120
           S RR  S+PPIPSGIFL+RP+P S  FDFV+L +NP   PFESL F+  ++GIVI QSCN
Sbjct: 61  SRRRSISNPPIPSGIFLARPYPDSLAFDFVNLTANPSRAPFESLNFTYQEYGIVINQSCN 120

Query: 121 GLLLCCNEGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVVCVRI 180
           GLLLC +      K DYYV+NPTTK  TTL   + R+I G+NLAFDPLRSS+YKV+CVR 
Sbjct: 121 GLLLCSSSYGNESKRDYYVHNPTTKHFTTLPKLQARKIFGLNLAFDPLRSSDYKVICVRN 180

Query: 181 LD-YSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVYLDLC 240
            D + NNY+IEIYS  SG WRPC G FS P + +FD GVYWN AVHWI  W NC+Y DL 
Sbjct: 181 SDSFKNNYEIEIYSSRSGPWRPCQGVFSAPSSMRFDSGVYWNSAVHWIDAWENCLYFDLR 240

Query: 241 EEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIESSGRQSV-LNVYEMEYDRCGWFEKY 300
           EEKLHELP+PR  DGWE R +EYFG+C G LH IE+   Q +  NVYEM+ D  GWF KY
Sbjct: 241 EEKLHELPMPRVPDGWEERRVEYFGSCGGNLHLIENYKPQDLEFNVYEMKDDHSGWFVKY 300

Query: 301 RIDLHTVSGLFPKMSPAPE--ESLNLCKFTVLAIVDQSKVDEDPYVILQGPRKLAVRLNV 360
           R+DLH VS  FP++ P+ E  E   + KF+V+ IVD  +V E+ Y++LQ   K+ VR+NV
Sbjct: 301 RVDLHCVSVAFPEIVPSEEDMEFGFIPKFSVITIVDGIEVGEESYLVLQIFEKI-VRVNV 360

Query: 361 KTGSFERI 365
           ++GSFER+
Sbjct: 361 ESGSFERL 365

BLAST of Tan0020275 vs. NCBI nr
Match: XP_022969759.1 (F-box protein At5g07610 [Cucurbita maxima])

HSP 1 Score: 426.8 bits (1096), Expect = 1.9e-115
Identity = 219/373 (58.71%), Postives = 275/373 (73.73%), Query Frame = 0

Query: 1   MLRCKAPRIVIRFSSSVAA----KSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLIS 60
           ML  K+P+I   F+++ AA    + V +NDDLLL+IL+RLPIRSLL FKSVSKRWL+LIS
Sbjct: 1   MLCSKSPKIHRNFTAATAATAAVEPVLNNDDLLLEILIRLPIRSLLTFKSVSKRWLALIS 60

Query: 61  DPNFSYRRITSHPPIPSGIFLSRPHPISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVIL 120
           +P+FS RR  S+PPIPSGIFL+RP+P S  FDFV+L +NP   PFESL F+  ++GIVI 
Sbjct: 61  NPSFSRRRSISNPPIPSGIFLARPYPDSLAFDFVNLSANPSRAPFESLNFTDQEYGIVIN 120

Query: 121 QSCNGLLLCCNEGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVV 180
           QSCNGLLLC +      K DYYV+NPTTK  TTL   + R+  G+NLAFDP RSS+YKV+
Sbjct: 121 QSCNGLLLCSSSYGNESKRDYYVHNPTTKHFTTLPKLQARKNFGLNLAFDPSRSSDYKVI 180

Query: 181 CVRILD-YSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVY 240
           CVR  D + NNY+IEIYS  SG WRPC G FS P + +FD GVYWN AVHWI  W NC+Y
Sbjct: 181 CVRNSDSFKNNYEIEIYSSRSGPWRPCQGVFSAPSSMRFDSGVYWNSAVHWIDAWENCLY 240

Query: 241 LDLCEEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIESSGRQSV-LNVYEMEYDRCGW 300
            DL EEKLHELP+PR  DGWE R +EYFG+C G LH IE+   Q +  NVYEM+ D  GW
Sbjct: 241 FDLREEKLHELPMPRVPDGWEERRVEYFGSCGGNLHLIENYEPQDLEFNVYEMKDDHSGW 300

Query: 301 FEKYRIDLHTVSGLFPKMSPAPEESLN---LCKFTVLAIVDQSKVDEDPYVILQGPRKLA 360
           F KYR+DLH VS  FP++ P+ EE+L    + KF+V+AIVD ++V E+ Y++LQ   K+ 
Sbjct: 301 FVKYRVDLHCVSVAFPEIIPS-EENLEFGFIPKFSVIAIVDGTEVGEESYIVLQIFEKI- 360

Query: 361 VRLNVKTGSFERI 365
           VR+NV++G FER+
Sbjct: 361 VRVNVESGRFERL 371

BLAST of Tan0020275 vs. NCBI nr
Match: KAG7017085.1 (F-box protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 426.0 bits (1094), Expect = 3.3e-115
Identity = 217/370 (58.65%), Postives = 272/370 (73.51%), Query Frame = 0

Query: 1   MLRCKAPRI--VIRFSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISDP 60
           ML  K+P+I      +++ A + V +NDDL L+IL+RLPIRSLL FKSVSKRWL+LIS+P
Sbjct: 1   MLCSKSPKIHRTSTAATTAAVEPVLNNDDLFLEILIRLPIRSLLTFKSVSKRWLALISNP 60

Query: 61  NFSYRRITSHPPIPSGIFLSRPHPISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVILQS 120
           +FS RR  S+PPIPSGIFL+RP+P S  FDFV+L +NP   PFESL F+  ++GIVI QS
Sbjct: 61  SFSRRRSISNPPIPSGIFLARPYPDSLAFDFVNLTANPSRAPFESLNFTDQEYGIVINQS 120

Query: 121 CNGLLLCCNEGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVVCV 180
           CNGLLLC +      K DYYV+NPTTK  TTL   + R+I G+NLAFDPLRSS+YKV+CV
Sbjct: 121 CNGLLLCSSSYGNESKRDYYVHNPTTKHFTTLPKLQARKIFGLNLAFDPLRSSDYKVICV 180

Query: 181 RILD-YSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVYLD 240
           R  D + NNY+IEIYS  SG WRPC G FS P + +FD GVYWN AVHWI  W NC+Y D
Sbjct: 181 RNSDSFKNNYEIEIYSSRSGPWRPCQGVFSAPSSMRFDSGVYWNSAVHWIDAWENCLYFD 240

Query: 241 LCEEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIESSGRQSV-LNVYEMEYDRCGWFE 300
           L EEKLHELP+PR  DGWE R +EYFG+C G LH IE+   Q +  NVYEM+ D  GWF 
Sbjct: 241 LREEKLHELPMPRVPDGWEERRVEYFGSCGGNLHLIENYKPQDLEFNVYEMKDDHSGWFV 300

Query: 301 KYRIDLHTVSGLFPKMSPAPE--ESLNLCKFTVLAIVDQSKVDEDPYVILQGPRKLAVRL 360
           KYR+DLH VS  FP++ P+ E  E   + KF+V+AIVD ++V E+ Y++LQ   K+ VR+
Sbjct: 301 KYRVDLHCVSVAFPEIVPSEENMEFGFIPKFSVIAIVDGTEVGEESYLVLQIFEKI-VRV 360

Query: 361 NVKTGSFERI 365
           NV++G FER+
Sbjct: 361 NVESGRFERL 369

BLAST of Tan0020275 vs. NCBI nr
Match: KAG6579630.1 (F-box protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 425.2 bits (1092), Expect = 5.7e-115
Identity = 216/370 (58.38%), Postives = 271/370 (73.24%), Query Frame = 0

Query: 1   MLRCKAPRI--VIRFSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISDP 60
           ML  K+P+I      +++ A + V +NDDL L+IL+RLPIRSLL FKSVSKRWL+LIS+P
Sbjct: 1   MLCSKSPKIHRTSTAATTAAVEPVLNNDDLFLEILIRLPIRSLLTFKSVSKRWLALISNP 60

Query: 61  NFSYRRITSHPPIPSGIFLSRPHPISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVILQS 120
           +FS RR  S+PPIPSGIFL+RP+P S  FDFV+L +NP   PFESL F+  ++GIVI QS
Sbjct: 61  SFSRRRSISNPPIPSGIFLARPYPDSLAFDFVNLTANPSRAPFESLNFTYQEYGIVINQS 120

Query: 121 CNGLLLCCNEGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVVCV 180
           CNGLLLC +      K DYYV+NPTTK  TTL   + R+I G+NLAFDPLRSS+YKV+CV
Sbjct: 121 CNGLLLCSSSYGNESKRDYYVHNPTTKHFTTLPKLQARKIFGLNLAFDPLRSSDYKVICV 180

Query: 181 RILD-YSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVYLD 240
           R  D + NNY+IEIYS  SG WRPC G FS P + +FD GVYWN AVHWI  W NC+Y D
Sbjct: 181 RNSDSFKNNYEIEIYSSRSGPWRPCQGVFSAPSSMRFDSGVYWNSAVHWIDAWENCLYFD 240

Query: 241 LCEEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIESSGRQSV-LNVYEMEYDRCGWFE 300
           L EEKLHELP+PR  DGWE R +EYFG+C G LH IE+   Q +  NVYEM+ D  GWF 
Sbjct: 241 LREEKLHELPMPRVPDGWEERRVEYFGSCGGNLHLIENYKPQDLEFNVYEMKDDHSGWFV 300

Query: 301 KYRIDLHTVSGLFPKMSPAPE--ESLNLCKFTVLAIVDQSKVDEDPYVILQGPRKLAVRL 360
           KYR+DLH VS  FP++ P+ E  E   + KF+V+ IVD ++V E+ Y++LQ   K+ VR+
Sbjct: 301 KYRVDLHCVSVAFPEIVPSEEDMEFGFIPKFSVITIVDGTEVGEESYLVLQIFEKI-VRV 360

Query: 361 NVKTGSFERI 365
           NV++G FER+
Sbjct: 361 NVESGRFERL 369

BLAST of Tan0020275 vs. NCBI nr
Match: XP_023549834.1 (F-box protein At5g07610 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 421.8 bits (1083), Expect = 6.3e-114
Identity = 216/371 (58.22%), Postives = 273/371 (73.58%), Query Frame = 0

Query: 1   MLRCKAPRI--VIRFSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISDP 60
           ML  K+P++      +++ A + V +N+DLLL+IL+RLPIRSLL FKSVSKRWL+LIS+P
Sbjct: 1   MLCSKSPKVHRTSTAATTAAVEPVLNNEDLLLEILIRLPIRSLLTFKSVSKRWLALISNP 60

Query: 61  NFSYRRITSHPPIPSGIFLSRPHPISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVILQS 120
           +FS RR  S+PPIPSGIFL+RP+P S  FDFV+L +NP   PFESL F+  ++GIVI QS
Sbjct: 61  SFSRRRSISNPPIPSGIFLARPYPDSLAFDFVNLSANPSRAPFESLNFTDQEYGIVINQS 120

Query: 121 CNGLLLCCNEGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVVCV 180
           CNGLLLC +      K DYYV+NPTTK  TTL   + R+I G+NLAFDPLRSS+YKV+CV
Sbjct: 121 CNGLLLCSSSYGNESKRDYYVHNPTTKHFTTLPKLQARKIFGLNLAFDPLRSSDYKVICV 180

Query: 181 RILD-YSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVYLD 240
           R  D + NNY+IEIYS  SG WRPC G FS P + +FD GVYWN AVHWI  W NC+Y D
Sbjct: 181 RNSDSFKNNYEIEIYSSRSGPWRPCQGVFSAPSSMRFDSGVYWNSAVHWIDAWENCLYFD 240

Query: 241 LCEEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIESSGRQS-VLNVYEMEYDRCGWFE 300
           L EEKLHELP+PR  +GWE R +EYFG+C G LH IE+   Q  V NVYEM+ D  GWF 
Sbjct: 241 LREEKLHELPMPRVPEGWEERRVEYFGSCGGNLHLIENYEPQDLVFNVYEMKDDHSGWFV 300

Query: 301 KYRIDLHTVSGLFPKMSPAPEESLN---LCKFTVLAIVDQSKVDEDPYVILQGPRKLAVR 360
           KYR+DL  VS  FP++ P+ EE+L    + KF+V+AIVD ++V E+ YV+LQ   K+ VR
Sbjct: 301 KYRVDLRCVSVAFPEIVPS-EENLEFGFIPKFSVIAIVDGTEVGEESYVVLQIFEKI-VR 360

Query: 361 LNVKTGSFERI 365
           +NV++G FE +
Sbjct: 361 VNVESGRFESL 369

BLAST of Tan0020275 vs. ExPASy TrEMBL
Match: A0A6J1ET91 (F-box protein At5g07610 OS=Cucurbita moschata OX=3662 GN=LOC111435776 PE=4 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 3.2e-116
Identity = 218/368 (59.24%), Postives = 271/368 (73.64%), Query Frame = 0

Query: 1   MLRCKAPRIVIRFSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNF 60
           ML  K+P+  I  +S+ A + V +NDDL L+IL+RLPIRSLL FKSVSKRWL+LIS+P+F
Sbjct: 1   MLCSKSPK--IHRTSTAAVEPVLNNDDLFLEILIRLPIRSLLTFKSVSKRWLALISNPSF 60

Query: 61  SYRRITSHPPIPSGIFLSRPHPISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVILQSCN 120
           S RR  S+PPIPSGIFL+RP+P S  FDFV+L +NP   PFESL F+  ++GIVI QSCN
Sbjct: 61  SRRRSISNPPIPSGIFLARPYPDSLAFDFVNLTANPSRAPFESLNFTYQEYGIVINQSCN 120

Query: 121 GLLLCCNEGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVVCVRI 180
           GLLLC +      K DYYV+NPTTK  TTL   + R+I G+NLAFDPLRSS+YKV+CVR 
Sbjct: 121 GLLLCSSSYGNESKRDYYVHNPTTKHFTTLPKLQARKIFGLNLAFDPLRSSDYKVICVRN 180

Query: 181 LD-YSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVYLDLC 240
            D + NNY+IEIYS  SG WRPC G FS P + +FD GVYWN AVHWI  W NC+Y DL 
Sbjct: 181 SDSFKNNYEIEIYSSRSGPWRPCQGVFSAPSSMRFDSGVYWNSAVHWIDAWENCLYFDLR 240

Query: 241 EEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIESSGRQSV-LNVYEMEYDRCGWFEKY 300
           EEKLHELP+PR  DGWE R +EYFG+C G LH IE+   Q +  NVYEM+ D  GWF KY
Sbjct: 241 EEKLHELPMPRVPDGWEERRVEYFGSCGGNLHLIENYKPQDLEFNVYEMKDDHSGWFVKY 300

Query: 301 RIDLHTVSGLFPKMSPAPE--ESLNLCKFTVLAIVDQSKVDEDPYVILQGPRKLAVRLNV 360
           R+DLH VS  FP++ P+ E  E   + KF+V+ IVD  +V E+ Y++LQ   K+ VR+NV
Sbjct: 301 RVDLHCVSVAFPEIVPSEEDMEFGFIPKFSVITIVDGIEVGEESYLVLQIFEKI-VRVNV 360

Query: 361 KTGSFERI 365
           ++GSFER+
Sbjct: 361 ESGSFERL 365

BLAST of Tan0020275 vs. ExPASy TrEMBL
Match: A0A6J1I3L0 (F-box protein At5g07610 OS=Cucurbita maxima OX=3661 GN=LOC111468861 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 9.4e-116
Identity = 219/373 (58.71%), Postives = 275/373 (73.73%), Query Frame = 0

Query: 1   MLRCKAPRIVIRFSSSVAA----KSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLIS 60
           ML  K+P+I   F+++ AA    + V +NDDLLL+IL+RLPIRSLL FKSVSKRWL+LIS
Sbjct: 1   MLCSKSPKIHRNFTAATAATAAVEPVLNNDDLLLEILIRLPIRSLLTFKSVSKRWLALIS 60

Query: 61  DPNFSYRRITSHPPIPSGIFLSRPHPISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVIL 120
           +P+FS RR  S+PPIPSGIFL+RP+P S  FDFV+L +NP   PFESL F+  ++GIVI 
Sbjct: 61  NPSFSRRRSISNPPIPSGIFLARPYPDSLAFDFVNLSANPSRAPFESLNFTDQEYGIVIN 120

Query: 121 QSCNGLLLCCNEGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVV 180
           QSCNGLLLC +      K DYYV+NPTTK  TTL   + R+  G+NLAFDP RSS+YKV+
Sbjct: 121 QSCNGLLLCSSSYGNESKRDYYVHNPTTKHFTTLPKLQARKNFGLNLAFDPSRSSDYKVI 180

Query: 181 CVRILD-YSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVY 240
           CVR  D + NNY+IEIYS  SG WRPC G FS P + +FD GVYWN AVHWI  W NC+Y
Sbjct: 181 CVRNSDSFKNNYEIEIYSSRSGPWRPCQGVFSAPSSMRFDSGVYWNSAVHWIDAWENCLY 240

Query: 241 LDLCEEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIESSGRQSV-LNVYEMEYDRCGW 300
            DL EEKLHELP+PR  DGWE R +EYFG+C G LH IE+   Q +  NVYEM+ D  GW
Sbjct: 241 FDLREEKLHELPMPRVPDGWEERRVEYFGSCGGNLHLIENYEPQDLEFNVYEMKDDHSGW 300

Query: 301 FEKYRIDLHTVSGLFPKMSPAPEESLN---LCKFTVLAIVDQSKVDEDPYVILQGPRKLA 360
           F KYR+DLH VS  FP++ P+ EE+L    + KF+V+AIVD ++V E+ Y++LQ   K+ 
Sbjct: 301 FVKYRVDLHCVSVAFPEIIPS-EENLEFGFIPKFSVIAIVDGTEVGEESYIVLQIFEKI- 360

Query: 361 VRLNVKTGSFERI 365
           VR+NV++G FER+
Sbjct: 361 VRVNVESGRFERL 371

BLAST of Tan0020275 vs. ExPASy TrEMBL
Match: A0A6J1DRL4 (F-box protein At5g07610 OS=Momordica charantia OX=3673 GN=LOC111023197 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 2.6e-113
Identity = 222/373 (59.52%), Postives = 269/373 (72.12%), Query Frame = 0

Query: 1   MLRCKAPRIVIRFSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNF 60
           ML  KAP+I+  F    AA+SV  + DLLL ILLRLPIRSLL+FKSVSKRWLSLIS+P+F
Sbjct: 48  MLCNKAPKILPGF----AAESVISDQDLLLLILLRLPIRSLLKFKSVSKRWLSLISNPHF 107

Query: 61  SYRRITSHPPIPSGIFLSRPHP-ISPEFDFVDLRSNPPSVPFESLQFSVPKHG---IVIL 120
           S+RR +SHPP PSGIFL RP P  SPE+DF+ L +N P  PFES+ F+  K G   + IL
Sbjct: 108 SHRRSSSHPPAPSGIFLPRPCPNFSPEYDFLSLSTNSPRAPFESIDFTERKQGTGTVQIL 167

Query: 121 QSCNGLLLCCNEGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVV 180
           QSCNGL+LC +  + + + DYYV NPTTKQ TTL   RT  I G+NLAFDP RSSNYKVV
Sbjct: 168 QSCNGLILCGSTNRNHSRRDYYVCNPTTKQFTTLPELRTGTIFGLNLAFDPSRSSNYKVV 227

Query: 181 CVRILD-YSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVY 240
           CVR  D ++NNYQIEIYS  +G WRPC G F+ P + +F  GVYWNGAVHWI  W NC+Y
Sbjct: 228 CVRDSDSFANNYQIEIYSSRTGPWRPCQGVFAAPISMRFHDGVYWNGAVHWISTWENCLY 287

Query: 241 LDLCEEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIE-SSGRQSVLNVYEMEYDRCGW 300
            DL EE+LHELP+PR  DGWE R   Y GNC GRL  IE    R    NVYEME DR GW
Sbjct: 288 FDLREERLHELPMPRVPDGWEERRTVYAGNCGGRLRLIEVYDPRGLQFNVYEMEDDRSGW 347

Query: 301 FEKYRIDLHTVSGLFPKMSPAPEESLNL---CKFTVLAIVDQSKVDEDPYVILQGPRKLA 360
           F KYRIDLH VS  FP++    EE+L L    KF+VLAI+D+S+ DE+ Y+++Q   K+ 
Sbjct: 348 FVKYRIDLHCVSAAFPEVI-CSEENLELGLFPKFSVLAIIDRSEGDEESYLVVQILGKV- 407

Query: 361 VRLNVKTGSFERI 365
           +R+NV++G FER+
Sbjct: 408 LRVNVESGRFERL 414

BLAST of Tan0020275 vs. ExPASy TrEMBL
Match: A0A6J1H2Y4 (F-box protein At5g07610-like OS=Cucurbita moschata OX=3662 GN=LOC111459522 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 2.6e-113
Identity = 226/371 (60.92%), Postives = 269/371 (72.51%), Query Frame = 0

Query: 5   KAPRIVIRFSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNFSYRR 64
           KAP+I   FSS+ AAKS+AD+DDLLL+ILLRLPIRSL+RFKSVSKRWLSLISDP F +RR
Sbjct: 5   KAPKITTPFSSA-AAKSLADDDDLLLEILLRLPIRSLIRFKSVSKRWLSLISDPTFCHRR 64

Query: 65  ITSHPPIPSGIFLSRPHP--ISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVILQSCNGL 124
           +T  P  PSGI L RPH    SP FDFV L  NPP VP ESL+ SVPK   VIL+SCNGL
Sbjct: 65  MTFQPSTPSGI-LCRPHSRRFSPGFDFVYLHPNPPRVPLESLEPSVPKRRFVILKSCNGL 124

Query: 125 LLCCNEGQTYPKTDYYVYNPTTKQ---CTTLQIDRTREIVGVNLAFDPLRSSNYKVVCVR 184
           LL    G++Y K DYYVYNPTTKQ      LQ+++    + +NLA+DPLRSSNYKVVCVR
Sbjct: 125 LL----GRSYRKNDYYVYNPTTKQYKKLPKLQMNKNSTSIYLNLAYDPLRSSNYKVVCVR 184

Query: 185 ILDYSNNYQIEIYSPDSGFWRPCNGRFSPP-YNTKFDYGVYWNGAVHWIRNWGNCVYLDL 244
           +LD S N++I+IYS DSGFWRPC G  S   +   F  GVYWNGA++W+R   NC+ LDL
Sbjct: 185 VLD-STNFEIKIYSSDSGFWRPCCGGSSKALFILGFSCGVYWNGALYWLRYLENCICLDL 244

Query: 245 CEEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIESSGRQSV-LNVYEMEYDR-CGWFE 304
           CEEKLH L IPR SDGW  RT++YFGN CGRL+WIE    +S  L VYEM  DR CGW  
Sbjct: 245 CEEKLHRLAIPRASDGWGDRTIKYFGNWCGRLYWIEGVDSESTELIVYEMGDDRGCGWSV 304

Query: 305 KYRIDLHTVSGLFPKMSPAPEESLNLCKFTVLAIVDQSKV-DEDPYVILQGPRKLAVRLN 364
           KYR+DL  V  LFP  S     +LN  KF+VLAIVDQS+V +E+PY++L      A+R+N
Sbjct: 305 KYRVDLQRVCSLFPDPSSGVRAALN-SKFSVLAIVDQSEVGEEEPYIVLLSGGN-AIRVN 364

Query: 365 VKTGSFERINY 367
           V +G FER+ Y
Sbjct: 365 VMSGRFERMQY 366

BLAST of Tan0020275 vs. ExPASy TrEMBL
Match: A0A0A0KPG1 (F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G167090 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 8.0e-99
Identity = 196/370 (52.97%), Postives = 249/370 (67.30%), Query Frame = 0

Query: 1   MLRCKAPRIVIRFSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNF 60
           ML  K PR      +S A + V  NDDL  QILLRLPIRSLLRFKSVSKRWLSLIS+PNF
Sbjct: 1   MLSSKLPR------TSPATEQVLINDDLFFQILLRLPIRSLLRFKSVSKRWLSLISNPNF 60

Query: 61  SYRRITSH--PPIPSGIFLSRPHPISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVILQS 120
           S+RR  SH   P PSGIF  RP P SP FDF++  +NP   PFES  F+  KHG +ILQS
Sbjct: 61  SHRRTISHHPNPTPSGIFFPRPTPKSPPFDFINFTTNPSRPPFESPHFADDKHGFLILQS 120

Query: 121 CNGLLLCCNEGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVVCV 180
           CNGL LC      Y   D+Y++NPTT   T L   +   + G+NLAFDPLRSS+YKV+CV
Sbjct: 121 CNGLFLCSTYNGNYSTRDFYIHNPTTNHHTKLPYLQVGAVFGLNLAFDPLRSSDYKVICV 180

Query: 181 RILD-YSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVYLD 240
           R  D Y++ +QIE+YS  +G WRP  G FS P + +FD GVYWN AVHWI    N +Y D
Sbjct: 181 RYSDAYTDTFQIEMYSSVTGPWRPVQGVFSAPLSMRFDSGVYWNNAVHWISTSENSLYFD 240

Query: 241 LCEEKLHELPIPRGSDGWEGRTMEYFGNCCGRLHWIESSGRQSV-LNVYEMEYDRCGWFE 300
           L EEK+H+LP+P   DG E R ++YFG C G L+ IE    Q + LNVY+M+ D CGWF 
Sbjct: 241 LREEKVHDLPMPGVPDGQEQRRVKYFGTCGGNLNLIEIYEAQEMELNVYQMQDDHCGWFV 300

Query: 301 KYRIDLHTVSGLFPKMSPAPEES-LNLC-KFTVLAIVDQSKVDEDPYVILQGPRKLAVRL 360
           +YR+DL  VS +FP+M P+ +++ L L  KF+V A+V+     E+  V+L+   K+ VR+
Sbjct: 301 RYRVDLRGVSVVFPEMIPSEDDADLGLFPKFSVAAVVEGI---EEASVVLEVDGKI-VRV 360

Query: 361 NVKTGSFERI 365
           NV++G FER+
Sbjct: 361 NVESGRFERL 360

BLAST of Tan0020275 vs. TAIR 10
Match: AT5G07610.1 (F-box family protein )

HSP 1 Score: 210.7 bits (535), Expect = 2.0e-54
Identity = 150/391 (38.36%), Postives = 214/391 (54.73%), Query Frame = 0

Query: 3   RCKAPRIVIR-----FSSSVAAKSVADNDDLLLQILLRLPIRSLLRFKSVSKRWLSLISD 62
           R KAPR         FSSS +A  VAD DD+L+QIL  LPI++LLRFK VSKRWLSLI++
Sbjct: 8   RTKAPRSARSRRNGGFSSS-SATIVADIDDVLIQILSFLPIKTLLRFKRVSKRWLSLITN 67

Query: 63  PNFSYRRITSHPPIP-SGIFLSRPHPISPEFDFVDLRSNPPSVPFES---LQFSVPKHGI 122
           P FS R I S+ P+P SG FL  P  I  ++ FV L  +  +    S   L F+  +  +
Sbjct: 68  PVFSNRVIKSNHPLPISGFFLHSPREI--KYSFVSLDDDATNQRISSSLPLWFTDHQTDM 127

Query: 123 VILQSCNGLLLC-CN-EGQTYPKTDYYVYNPTTKQCTTLQIDRTREIVGVNLAFDPLRSS 182
           +I+QS NGLLLC C+     +  T+YYVYNPTTKQ T L   +    + ++LAFDP RS 
Sbjct: 128 IIMQSTNGLLLCKCSCASSNHFNTNYYVYNPTTKQYTLLH--QIAGHIALSLAFDPSRSP 187

Query: 183 NYKVVCVRILDYSNN----------YQIEIYSPDSGFW-RPCNGRFSPPYNTKFDYGVYW 242
           +YKV C+R    SNN          Y IE+YS + G W R      SP    +F Y V+W
Sbjct: 188 HYKVFCLR--GRSNNSFSSASDSELYHIEVYSSNEGLWRRVVPVPTSPSTFIEFSYSVFW 247

Query: 243 NGAVHWIR-NWGNCVYLDLCEEKLHELPIPRGSDGWE----GRTMEYFGNCCGRLHWIES 302
           NGAV+W   +  +C+  D+  +++  LP+P      E     R + +     G L++IE 
Sbjct: 248 NGAVNWYGFSSRDCLSFDINTQEIKILPLPDHEHEDEPLPDPRILMFLDESQGNLYYIEV 307

Query: 303 SGRQSV-LNVYEMEYDRCGWFEKYRIDLHTVSGLFPKMSPAP-EESLNLCKFTVLAIVDQ 362
           + + S  L VYEME +   W  KY +DL  ++  FP+M          +  F+V+  V +
Sbjct: 308 NNQSSSNLRVYEMESNSSSWSVKYNVDLEPLAAAFPEMIRTEYYTDRRIYAFSVIGFV-K 367

Query: 363 SKVDEDPYVILQGPRKLAVRLNVKTGSFERI 365
            + D   Y++L  P + AV+ N    +F+++
Sbjct: 368 EETDAASYILLHIPNQ-AVKYNFIDKTFKKL 389

BLAST of Tan0020275 vs. TAIR 10
Match: AT1G12870.1 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 73.9 bits (180), Expect = 3.0e-13
Identity = 72/253 (28.46%), Postives = 117/253 (46.25%), Query Frame = 0

Query: 26  DDLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNFSYRRI----TSHPPIPSGIFLSRPH 85
           DD++ +I L+LP+++L+RFKS+SK+W S +    FS R +     SH   P  + ++   
Sbjct: 35  DDVVEEIFLKLPVKALMRFKSLSKQWRSTLESCYFSQRHLKIAERSHVDHPKVMIITEKW 94

Query: 86  PISPEFDFVDLRSNPPSVPF-ESLQFSVPK---HGIVILQSCNGLLLCCNEGQTYPKT-D 145
             +P+ +      +  SV F  S  F+ P+   H I   +SC+G + C +     PKT D
Sbjct: 95  --NPDIEISFRTISLESVSFLSSALFNFPRGFHHPIYASESCDG-IFCIHS----PKTQD 154

Query: 146 YYVYNPTTKQCTTL--------------QIDRTREIVGVN-LAFDPLRSSNYKVVCVRIL 205
            YV NP T+    L               +D  R+++ VN LAF  +++++YK+V +   
Sbjct: 155 IYVVNPATRWFRQLPPARFQIFMHKLNPTLDTLRDMIPVNHLAF--VKATDYKLVWLYNS 214

Query: 206 DYSNNYQIEIYSPDSGFWRPCNGRFSPPYNTKFDY-GVYWNGAVHWIRNWGNC----VYL 250
           D S   + E++   +  WR       P Y    D      NG ++W     N     + L
Sbjct: 215 DASRVTKCEVFDFKANAWRYLT--CIPSYRIYHDQKPASANGTLYWFTETYNAEIKVIAL 274

BLAST of Tan0020275 vs. TAIR 10
Match: AT5G15660.1 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 67.8 bits (164), Expect = 2.1e-11
Identity = 64/220 (29.09%), Postives = 100/220 (45.45%), Query Frame = 0

Query: 27  DLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNF--SYRRITSHPP---IPSGIFLSRPH 86
           DL+++IL RLP++S+ RF +VSK W + I  P+F  SYR  +S  P   I S +    P+
Sbjct: 31  DLVIEILERLPLKSVARFLTVSKLWATTIRSPDFRKSYRGGSSSEPRTLIVSDLNFKEPN 90

Query: 87  PISPEFDFVDLRSNPPSVPFESLQFSVPKHGIVILQSCNGLLLCCNEGQTYPKTDYYVYN 146
           P      F    S+P  +   +  F+ P+H        NGL+           TD  V N
Sbjct: 91  P--KLHFFRPSISSPSFLSSLTCPFTYPRHEEYYYHHVNGLI------SVGYGTDQIVIN 150

Query: 147 PTTKQCTTLQIDRTREIVGVNLAFDPLRSSNYKVVCV--RILDY----SNNYQIEIYSPD 206
           PTT +  TL   +TR  + ++       S  YKV+C+  R+  +    S+ +Q+      
Sbjct: 151 PTTGKFITLPRPKTRRKLVISFFGYDSVSDQYKVLCMTERLRGHPEEASSQHQVYTLGAK 210

Query: 207 SGFWRPCNGRFSPPYNTKFDYGVYWNGAVHWIRNWGNCVY 236
              W+  N   S P+       V  NG V++I   G  ++
Sbjct: 211 QKSWKMIN--CSIPHRPWSWNAVCINGVVYYIAKTGEGMF 240

BLAST of Tan0020275 vs. TAIR 10
Match: AT4G04690.1 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 63.2 bits (152), Expect = 5.3e-10
Identity = 55/205 (26.83%), Postives = 87/205 (42.44%), Query Frame = 0

Query: 27  DLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNFSYRRITSHPPIPSGIFLSRPHPISPE 86
           +L+ +IL + P  SL RFKS  K+W  +I+   F Y  +   P     I   +   I   
Sbjct: 14  ELVEEILKKTPAESLNRFKSTCKQWYGIITSKRFMYNHLDHSPERFIRIDDHKTVQIMDP 73

Query: 87  FDFVDLRSNPPSVPFESLQFSVPKHGIVILQSCNGLLLCCNEGQTYPKT---DYYVYNPT 146
              +   S  P V      F  P H    +  C+GL+LC     +Y +T   +  V+NP 
Sbjct: 74  MTGIFSDSPVPDV------FRSP-HSFASMVHCDGLMLCICSDSSYERTREANLAVWNPV 133

Query: 147 TKQCTTLQ-IDRTREIVGVNLAFDPLRSSNYKVVCVRILDYSNNYQIEIYSPDSGFWRPC 206
           TK+   ++ +D   E     + +D     NYK+V        ++ + EIY   S  WR  
Sbjct: 134 TKKIKWIEPLDSYYETDYFGIGYDNTCRENYKIVRFSGPMSFDDTECEIYEFKSDSWRTL 193

Query: 207 NGRFSPPYNTKFDYGVYWNGAVHWI 228
           + ++   Y      GV   G ++WI
Sbjct: 194 DTKYWDVYTQ--CRGVSVKGNMYWI 209

BLAST of Tan0020275 vs. TAIR 10
Match: AT4G17780.1 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 62.8 bits (151), Expect = 6.9e-10
Identity = 82/306 (26.80%), Postives = 128/306 (41.83%), Query Frame = 0

Query: 27  DLLLQILLRLPIRSLLRFKSVSKRWLSLISDPNFSYRRITSHPPIPSGIFLSRPHPISPE 86
           DLL  I LRLP++S+L  KSVSKRW S++    F  RR++          L +   I   
Sbjct: 16  DLLEDIFLRLPLKSILISKSVSKRWRSILESKTFVERRMS----------LQKKRKILAA 75

Query: 87  FDFV---DLRSNPPSVPFESLQFSVPKHGIVILQS--CNGLLLCCNEGQTYPKTDYYVYN 146
           ++     + R  P S   +  +  V  H      S  C+G L+C  E +        V N
Sbjct: 76  YNCKCGWEPRLLPGSSQCKGNEEIVYLHCNAAQPSFTCDG-LVCILEPRWID-----VLN 135

Query: 147 PTTKQCTTLQIDRTREIVGVNLAFDPLR----------SSNYKVVCVRILDYSN------ 206
           P T+Q           I GV  AF P            + +YKVV + ++ +S       
Sbjct: 136 PWTRQLRRYGFG-FGTIFGVGSAFSPRHWAMGFGKDKVTGSYKVVKMCLISFSEICARDP 195

Query: 207 NYQIEIYSPDSGFWRPCNGRFSPPYNT-KFDYGVYWNGAVHWI-----RNWGNCVYLDLC 266
             +  +   ++G WR  +    PPY   +       NG+++W+     R W   + LDL 
Sbjct: 196 EVEYSVLDVETGEWRMLS---PPPYKVFEVRKSECANGSIYWLHKPTERAW-TILALDLH 255

Query: 267 EEKLHELPIPRGSDGWEGRTMEYFG--NCCGRLHWIES-SGRQSVLNVYEMEYDRCGWFE 303
           +E+LH + +P  S      T E F   N   RL    + +  +  L ++ M+ +   W +
Sbjct: 256 KEELHNISVPDMS-----VTQETFQIVNLEDRLAIANTYTKTEWKLEIWSMDTEVETWTK 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLS02.9e-5338.36F-box protein At5g07610 OS=Arabidopsis thaliana OX=3702 GN=At5g07610 PE=2 SV=1[more]
Q9LPW24.2e-1228.46Putative F-box/kelch-repeat protein At1g12870 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9LFW03.0e-1029.09Putative F-box protein At5g15660 OS=Arabidopsis thaliana OX=3702 GN=At5g15660 PE... [more]
Q9S9V17.4e-0926.83Putative F-box only protein 15 OS=Arabidopsis thaliana OX=3702 GN=FBX15 PE=4 SV=... [more]
Q3E9Z89.7e-0926.80Putative F-box protein At4g17780 OS=Arabidopsis thaliana OX=3702 GN=At4g17780 PE... [more]
Match NameE-valueIdentityDescription
XP_022929085.16.7e-11659.24F-box protein At5g07610 [Cucurbita moschata][more]
XP_022969759.11.9e-11558.71F-box protein At5g07610 [Cucurbita maxima][more]
KAG7017085.13.3e-11558.65F-box protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6579630.15.7e-11558.38F-box protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023549834.16.3e-11458.22F-box protein At5g07610 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1ET913.2e-11659.24F-box protein At5g07610 OS=Cucurbita moschata OX=3662 GN=LOC111435776 PE=4 SV=1[more]
A0A6J1I3L09.4e-11658.71F-box protein At5g07610 OS=Cucurbita maxima OX=3661 GN=LOC111468861 PE=4 SV=1[more]
A0A6J1DRL42.6e-11359.52F-box protein At5g07610 OS=Momordica charantia OX=3673 GN=LOC111023197 PE=4 SV=1[more]
A0A6J1H2Y42.6e-11360.92F-box protein At5g07610-like OS=Cucurbita moschata OX=3662 GN=LOC111459522 PE=4 ... [more]
A0A0A0KPG18.0e-9952.97F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G167090 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT5G07610.12.0e-5438.36F-box family protein [more]
AT1G12870.13.0e-1328.46F-box and associated interaction domains-containing protein [more]
AT5G15660.12.1e-1129.09F-box and associated interaction domains-containing protein [more]
AT4G04690.15.3e-1026.83F-box and associated interaction domains-containing protein [more]
AT4G17780.16.9e-1026.80F-box and associated interaction domains-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 26..64
e-value: 0.0011
score: 28.2
IPR001810F-box domainPFAMPF00646F-boxcoord: 26..65
e-value: 1.4E-7
score: 31.2
IPR001810F-box domainPROSITEPS50181FBOXcoord: 26..65
score: 8.729132
IPR017451F-box associated interaction domainTIGRFAMTIGR01640TIGR01640coord: 117..252
e-value: 5.5E-15
score: 53.3
NoneNo IPR availableGENE3D1.20.1280.50coord: 25..60
e-value: 2.3E-8
score: 35.9
NoneNo IPR availablePANTHERPTHR47993:SF152FAMILY PROTEIN, PUTATIVE-RELATEDcoord: 15..340
NoneNo IPR availablePANTHERPTHR47993OS09G0372900 PROTEIN-RELATEDcoord: 15..340
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 26..138
IPR011043Galactose oxidase/kelch, beta-propellerSUPERFAMILY50965Galactose oxidase, central domaincoord: 118..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0020275.1Tan0020275.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding