Tan0020075 (gene) Snake gourd v1

Overview
NameTan0020075
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionprotein NRT1/ PTR FAMILY 5.10-like
LocationLG10: 67376367 .. 67379135 (-)
RNA-Seq ExpressionTan0020075
SyntenyTan0020075
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAACTCAAAATCTGTAAAAGGTCGTAGAATTGAAATTTTTTTAAAATTAAAGACTAAATTGAGAGTTACCGGAAAAGTCACAAACCACTTCGTTAATCATTTCTGAATCGGAAATTTGGAACTGCGGCGGAGAAATCCTTTAAAAATGGTGTAACATCCCCCCATGGTGATAACGTGGGGGCACTGTAGCATATTCTTCCTCCTTCCTCCTTCCTCCTTCCTCCTTCCTCTTTCCGCTTCGCTTCCGATCAAAAATGGACCTCTCAGTCTCACTTTCCGACTCTCCACTTCTGACCGACACCGTCGACGGCGCCGTTGATTTTCAAGGTCAGCCAGTTCTCCGATCCTCCTCCGGGGGCTGGAGGTCCGCCTCTTTCATGATAGGTAATAATACCATTGATATTTACTCGTTTTGTGATGGTGATGTTGTGAACTAAATTTACGATCCATTTAGGAGTGGAGGTCGCCGAGAGATTTGCTTACTACGGAATCGGTACCAATCTCATAACTTACCTTACCGGTCCACTTGGTCAGTCTGTAGCCACCGCCGCCGAGAGTGTCAACATCTGGTCAGGCATCTCCATGCTATTCACTCTCCTCGGAGCCTTTCTCGCCGACTCCTTCTTCGGACGATATCGCACCATTCTTTTCTCTTCTGCAATTTACGTCTTGGTCTGCTCTCTATCTTATCTTTCTTCCTTGTTTCAATCACTTTTCTCACTTCACATTCTCATCCACTTGAAATTCAAAACTCCTTACAGGGACTAGCCTTCCTCTCTTTCTCTGCTCTGCTTCCCCTCTGTCGGAACCCTAACGAAATTTCATCATGTTCCACTCCCCAGTTTCAGCTTGTGTTCTTCTTCGTCTCTCTCTATCTAATAGGGATAGGGCAAGGTGGCCACAAGCCTTGCGTTCAGGCTTTTGGAGCCGATCAGTTCGATGCCCAACACCCACAAGAGGCCAAATCCAAAAGCTCCTTCTTCAATTGGTGGTTTTTTGGTATCTGCGCTGGGACCTTTGTAGCCATTTTGGTTGTCACATATACAGAGGAAAACCTTAGTTGGAGTTTGGGCTTTGGAATTCCTTGTATTATGATGGTCATTGCATCACTTGTCTTTGTATTTGGGACCAACACGTATCGTTATAGCGTCAAAAAGTATGATAACAGCCCATTTGTGAGAATTGGTCGGGTATTTGTGTCTGCAGTTAGAAATTGGAGAGCTTCTTCAACGATCATCTTTGATCAGGAAGATGGTGCTACAGATTTCTCACAACAGAATGCTGGACAGTTCAGGTAACAATATTACCAAATTCTTGCCCCATATGATATGGAGTTTAGAGTAATTATAAACTTTAGATTATGCTTCTATTTTCTCTTATTGTCAATACAATTATTGAAATGCTAACATGAGTTAGAGTTCATAATGCTGGAAGTGCAGGACCAAATTTATGACTTCAATAATTAGGTTCACCATGAAATGAAGTGGGTGGAATCCTGTCCAGTTCAATCAAATTGCAAATTTGCTAAGTTACAATGTTAATGTATATATGTAGTTCAATCAAATTTAACAGACATTTGTTGACAATTGTTGGTTCCTCAAATGTGGCAGATTTCTTAACAAAGCTTGCATTGTCCCCAATCATTCGAACCAAAGCGGAATGGTATGCAGCGTCCGTGAGGTTGAAGAAGCAAAGGCAGTTCTTAGGATTTTTCCAGTATGGATGACAGTCCTTGTATTCGGTATTGTGTTTGCACAAGATTCCACATTCTTCACCAAGCAAGGAGCTACAATGGATAGAACAATTTTATCAGGCTTTATCATTCCTGCTGCTGCAATCGATTCATTCATTCCTCTTTCCATTGTTATCTTTATTACTATCTATGATCTTGTATTTGTTCCTATCGCCAGAGCTTTTACAGGAATACAGTCTGGCATCACAACCCTGCAGCGAATAGGAACGGGGCTGGTTATATCTGTGATTTCAATGTTGGTTGCAACTATGGTTGAGAGGAAAAGATTAAGAATTGCAGAAGAACACGGTCTAGTCGATAGGCCTGAGATAACAATTCCAATGAGCTTTTGGTGGTTAGTTCCTCAATATATCTTGTATGGTTTGGCTGAAGTTTTTACATTAGTTGGTCTGCAAGAGTTTTTCTACGATCAATGTCCAACAGATTTGAAAAGCATGGGGCTTGCCTTTTACACAAGCGTGTTAGGGATGGGAAGCATTTTAAGCAGTCTTTTAGTTTCTGCTATTGATGAAGCAACTGGAGCCAATGGGCAGAATAGTTGGTTTTCCAACAATCTCAACAAGGCGCATCTTGATTACTTTTATTTGCTATTGTCTGGACTCAGTGTTCTAGCGCTTGTTGCCTTCCTATTTGTTTCCAACTCACATGTTCATAGCAGATGAAGAAGTATCACTACAACAATTCTTATCTATTGCAGGCTTGCAGCCTTGTATTATAGCGTTTACACAGAAAGAAAGGCTATAGATTTAGACACCATTCAAGAACTTGGATGCTGTTCATAATAGAGTGGAAGAGAACAGACTTGTAAGCTCCTCATTGTTCATCATATTCATCAGCCTGAAGCTTCTTATGCGAGGATAATGAACTATAAATCTGGAAAAGCTTCAGATTCAAGTATGTTCTTTTTCCTGAATTTGATATGCCCTTTCGTCAACCAAGCTGTTGCATGGTTTCTTTTTTGGGTAAATACATAGATAGATCATATTTTCAATTCTGTCCAACATCACTATTT

mRNA sequence

AAAAAAACTCAAAATCTGTAAAAGGTCGTAGAATTGAAATTTTTTTAAAATTAAAGACTAAATTGAGAGTTACCGGAAAAGTCACAAACCACTTCGTTAATCATTTCTGAATCGGAAATTTGGAACTGCGGCGGAGAAATCCTTTAAAAATGGTGTAACATCCCCCCATGGTGATAACGTGGGGGCACTGTAGCATATTCTTCCTCCTTCCTCCTTCCTCCTTCCTCCTTCCTCTTTCCGCTTCGCTTCCGATCAAAAATGGACCTCTCAGTCTCACTTTCCGACTCTCCACTTCTGACCGACACCGTCGACGGCGCCGTTGATTTTCAAGGTCAGCCAGTTCTCCGATCCTCCTCCGGGGGCTGGAGGTCCGCCTCTTTCATGATAGGAGTGGAGGTCGCCGAGAGATTTGCTTACTACGGAATCGGTACCAATCTCATAACTTACCTTACCGGTCCACTTGGTCAGTCTGTAGCCACCGCCGCCGAGAGTGTCAACATCTGGTCAGGCATCTCCATGCTATTCACTCTCCTCGGAGCCTTTCTCGCCGACTCCTTCTTCGGACGATATCGCACCATTCTTTTCTCTTCTGCAATTTACGTCTTGGGACTAGCCTTCCTCTCTTTCTCTGCTCTGCTTCCCCTCTGTCGGAACCCTAACGAAATTTCATCATGTTCCACTCCCCAGTTTCAGCTTGTGTTCTTCTTCGTCTCTCTCTATCTAATAGGGATAGGGCAAGGTGGCCACAAGCCTTGCGTTCAGGCTTTTGGAGCCGATCAGTTCGATGCCCAACACCCACAAGAGGCCAAATCCAAAAGCTCCTTCTTCAATTGGTGGTTTTTTGGTATCTGCGCTGGGACCTTTGTAGCCATTTTGGTTGTCACATATACAGAGGAAAACCTTAGTTGGAGTTTGGGCTTTGGAATTCCTTGTATTATGATGGTCATTGCATCACTTGTCTTTGTATTTGGGACCAACACGTATCGTTATAGCGTCAAAAAGTATGATAACAGCCCATTTGTGAGAATTGGTCGGGTATTTGTGTCTGCAGTTAGAAATTGGAGAGCTTCTTCAACGATCATCTTTGATCAGGAAGATGGTGCTACAGATTTCTCACAACAGAATGCTGGACAGTTCAGATTTCTTAACAAAGCTTGCATTGTCCCCAATCATTCGAACCAAAGCGGAATGGTATGCAGCGTCCGTGAGGTTGAAGAAGCAAAGGCAGTTCTTAGGATTTTTCCAGTATGGATGACAGTCCTTGTATTCGGTATTGTGTTTGCACAAGATTCCACATTCTTCACCAAGCAAGGAGCTACAATGGATAGAACAATTTTATCAGGCTTTATCATTCCTGCTGCTGCAATCGATTCATTCATTCCTCTTTCCATTGTTATCTTTATTACTATCTATGATCTTGTATTTGTTCCTATCGCCAGAGCTTTTACAGGAATACAGTCTGGCATCACAACCCTGCAGCGAATAGGAACGGGGCTGGTTATATCTGTGATTTCAATGTTGGTTGCAACTATGGTTGAGAGGAAAAGATTAAGAATTGCAGAAGAACACGGTCTAGTCGATAGGCCTGAGATAACAATTCCAATGAGCTTTTGGTGGTTAGTTCCTCAATATATCTTGTATGGTTTGGCTGAAGTTTTTACATTAGTTGGTCTGCAAGAGTTTTTCTACGATCAATGTCCAACAGATTTGAAAAGCATGGGGCTTGCCTTTTACACAAGCGTGTTAGGGATGGGAAGCATTTTAAGCAGTCTTTTAGTTTCTGCTATTGATGAAGCAACTGGAGCCAATGGGCAGAATAGTTGGTTTTCCAACAATCTCAACAAGGCGCATCTTGATTACTTTTATTTGCTATTGTCTGGACTCAGTGTTCTAGCGCTTGTTGCCTTCCTATTTGTTTCCAACTCACATGTTCATAGCAGATGAAGAAGTATCACTACAACAATTCTTATCTATTGCAGGCTTGCAGCCTTGTATTATAGCGTTTACACAGAAAGAAAGGCTATAGATTTAGACACCATTCAAGAACTTGGATGCTGTTCATAATAGAGTGGAAGAGAACAGACTTGTAAGCTCCTCATTGTTCATCATATTCATCAGCCTGAAGCTTCTTATGCGAGGATAATGAACTATAAATCTGGAAAAGCTTCAGATTCAAGTATGTTCTTTTTCCTGAATTTGATATGCCCTTTCGTCAACCAAGCTGTTGCATGGTTTCTTTTTTGGGTAAATACATAGATAGATCATATTTTCAATTCTGTCCAACATCACTATTT

Coding sequence (CDS)

ATGGACCTCTCAGTCTCACTTTCCGACTCTCCACTTCTGACCGACACCGTCGACGGCGCCGTTGATTTTCAAGGTCAGCCAGTTCTCCGATCCTCCTCCGGGGGCTGGAGGTCCGCCTCTTTCATGATAGGAGTGGAGGTCGCCGAGAGATTTGCTTACTACGGAATCGGTACCAATCTCATAACTTACCTTACCGGTCCACTTGGTCAGTCTGTAGCCACCGCCGCCGAGAGTGTCAACATCTGGTCAGGCATCTCCATGCTATTCACTCTCCTCGGAGCCTTTCTCGCCGACTCCTTCTTCGGACGATATCGCACCATTCTTTTCTCTTCTGCAATTTACGTCTTGGGACTAGCCTTCCTCTCTTTCTCTGCTCTGCTTCCCCTCTGTCGGAACCCTAACGAAATTTCATCATGTTCCACTCCCCAGTTTCAGCTTGTGTTCTTCTTCGTCTCTCTCTATCTAATAGGGATAGGGCAAGGTGGCCACAAGCCTTGCGTTCAGGCTTTTGGAGCCGATCAGTTCGATGCCCAACACCCACAAGAGGCCAAATCCAAAAGCTCCTTCTTCAATTGGTGGTTTTTTGGTATCTGCGCTGGGACCTTTGTAGCCATTTTGGTTGTCACATATACAGAGGAAAACCTTAGTTGGAGTTTGGGCTTTGGAATTCCTTGTATTATGATGGTCATTGCATCACTTGTCTTTGTATTTGGGACCAACACGTATCGTTATAGCGTCAAAAAGTATGATAACAGCCCATTTGTGAGAATTGGTCGGGTATTTGTGTCTGCAGTTAGAAATTGGAGAGCTTCTTCAACGATCATCTTTGATCAGGAAGATGGTGCTACAGATTTCTCACAACAGAATGCTGGACAGTTCAGATTTCTTAACAAAGCTTGCATTGTCCCCAATCATTCGAACCAAAGCGGAATGGTATGCAGCGTCCGTGAGGTTGAAGAAGCAAAGGCAGTTCTTAGGATTTTTCCAGTATGGATGACAGTCCTTGTATTCGGTATTGTGTTTGCACAAGATTCCACATTCTTCACCAAGCAAGGAGCTACAATGGATAGAACAATTTTATCAGGCTTTATCATTCCTGCTGCTGCAATCGATTCATTCATTCCTCTTTCCATTGTTATCTTTATTACTATCTATGATCTTGTATTTGTTCCTATCGCCAGAGCTTTTACAGGAATACAGTCTGGCATCACAACCCTGCAGCGAATAGGAACGGGGCTGGTTATATCTGTGATTTCAATGTTGGTTGCAACTATGGTTGAGAGGAAAAGATTAAGAATTGCAGAAGAACACGGTCTAGTCGATAGGCCTGAGATAACAATTCCAATGAGCTTTTGGTGGTTAGTTCCTCAATATATCTTGTATGGTTTGGCTGAAGTTTTTACATTAGTTGGTCTGCAAGAGTTTTTCTACGATCAATGTCCAACAGATTTGAAAAGCATGGGGCTTGCCTTTTACACAAGCGTGTTAGGGATGGGAAGCATTTTAAGCAGTCTTTTAGTTTCTGCTATTGATGAAGCAACTGGAGCCAATGGGCAGAATAGTTGGTTTTCCAACAATCTCAACAAGGCGCATCTTGATTACTTTTATTTGCTATTGTCTGGACTCAGTGTTCTAGCGCTTGTTGCCTTCCTATTTGTTTCCAACTCACATGTTCATAGCAGATGA

Protein sequence

MDLSVSLSDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSFSALLPLCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQEAKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTYRYSVKKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIVPNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSGFIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLVATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGLSVLALVAFLFVSNSHVHSR
Homology
BLAST of Tan0020075 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 9.5e-186
Identity = 322/559 (57.60%), Postives = 427/559 (76.39%), Query Frame = 0

Query: 4   SVSLSDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITY 63
           +V  + +PLL  T    VD++ +P ++SSSGGWRSA F+IGVEVAERFAYYGI +NLITY
Sbjct: 6   AVDEAGTPLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITY 65

Query: 64  LTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSF 123
           LTGPLGQS A AA +VN WSG + L  LLGAF+ADSF GR+RTIL +SA+Y++GL  L+ 
Sbjct: 66  LTGPLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTL 125

Query: 124 SALLPL-CRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQE 183
           SA++P  C+  N +SSCS P+FQ++ FF +LYL+ + QGGHKPCVQAFGADQFD + P+E
Sbjct: 126 SAMIPSDCKVSNLLSSCS-PRFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEE 185

Query: 184 AKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTY 243
            K+KSSFFNWW+FG+C GT   + V+ Y ++NLSW+LGFGIPCI MV+A +V + GT TY
Sbjct: 186 CKAKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTY 245

Query: 244 RYSVKKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIV 303
           R+S+++ D SPFVRIG V+V+AV+NW  S+  +   E+     S  ++ QF FLNKA + 
Sbjct: 246 RFSIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVA 305

Query: 304 PNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSG 363
            N S      CS+ E+EEAK+VLR+ P+W+T LV+ +VFAQ  TFFTKQGATM+R+I  G
Sbjct: 306 KNGS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPG 365

Query: 364 FIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLV 423
           + I  A + SFI LSIVIFI IYD V +PIAR+FT    GIT LQRIGTG+ +S ++M+V
Sbjct: 366 YKISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVV 425

Query: 424 ATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPT 483
           A +VE KRL+ A ++GLVD P+ T+PMS WWLVPQY+L+G+ +VF +VGLQEFFYDQ P 
Sbjct: 426 AALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPN 485

Query: 484 DLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGL 543
           +L+S+GLA Y S+ G+G+ LSS ++S I++AT  +GQ SWF+NNLN+AHLDYFY LL+ L
Sbjct: 486 ELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACL 545

Query: 544 SVLALVAFLFVSNSHVHSR 562
           S + L ++L+V+ S+V  R
Sbjct: 546 SFIGLASYLYVAKSYVSKR 553

BLAST of Tan0020075 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 610.1 bits (1572), Expect = 2.4e-173
Identity = 316/556 (56.83%), Postives = 405/556 (72.84%), Query Frame = 0

Query: 8   SDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITYLTGP 67
           S+  L  + V  AVD +G    RS++G WR+A F+IGVEVAERFAYYGIG+NLI+YLTGP
Sbjct: 5   SEISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGP 64

Query: 68  LGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSFSALL 127
           LG+S A AA +VN WSGI+ L  +LGAF+AD+F GRYRTI+ SS IYVLGLAFL+ SA  
Sbjct: 65  LGESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAF- 124

Query: 128 PLCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQEAKSKS 187
            L  N  E++S ST  F  V FF SLYL+ IGQ GHKPCVQAFGADQFD +  QE   +S
Sbjct: 125 -LIPNTTEVTS-STSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRS 184

Query: 188 SFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTYRYSVK 247
           SFFNWW+  + AG   AILVV Y +E  SW+ GFGIPC+ MVI+ ++FV G   YRYS +
Sbjct: 185 SFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKR 244

Query: 248 KYDN--SPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIVPNH 307
           +++   +PF RIGRVF  A++N R SS+ +   E  A    ++ +    F NKA +VPN 
Sbjct: 245 RHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQS----FFNKALLVPND 304

Query: 308 SNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSGFII 367
           S+Q        +VE+A A++R+ PVW T L + I +AQ  TFFTKQG TMDRTIL G  I
Sbjct: 305 SSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKI 364

Query: 368 PAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLVATM 427
           P A++  FI +SIV+F+ IYD VFVPIAR  T    GITTL+RIGTG+V+S I+M++A +
Sbjct: 365 PPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAAL 424

Query: 428 VERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPTDLK 487
           VE KRL  A+EHGL+D+PE T+PMS WWL+PQY+L GLA+V+TLVG+QEFFY Q PT+L+
Sbjct: 425 VEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELR 484

Query: 488 SMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGLSVL 547
           S+GLA Y S LG+GS+LSSLL+S ID ATG +  NSWF++NLN+AHLDYFY LL+ +S +
Sbjct: 485 SIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAV 544

Query: 548 ALVAFLFVSNSHVHSR 562
               FLF+S S+++ R
Sbjct: 545 GFFTFLFISKSYIYRR 553

BLAST of Tan0020075 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 602.1 bits (1551), Expect = 6.6e-171
Identity = 309/556 (55.58%), Postives = 407/556 (73.20%), Query Frame = 0

Query: 8   SDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITYLTGP 67
           S + L  + V  AVD +G    RS +G WR+A F+IGVEVAERFA YGIG+NLI+YLTGP
Sbjct: 5   SKTSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGP 64

Query: 68  LGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSFSALL 127
           LGQS A AA +VN WSGIS +  LLGAF+AD+F GRY TI+ +S IYVLGLAFL+ SA  
Sbjct: 65  LGQSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAF- 124

Query: 128 PLCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQEAKSKS 187
            L  N  E++S S   F    FF SLYL+ IGQ GHKPCVQAFGADQFD ++PQE   +S
Sbjct: 125 -LIPNNTEVTS-SPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDRS 184

Query: 188 SFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTYRYSV- 247
           SFFNWW+  +CAG  +AILVV Y +EN+SW+LGFGIPC+ MVI+ ++FV G  +YR+S  
Sbjct: 185 SFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSKT 244

Query: 248 -KKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIVPNH 307
            ++ + +PF RIGRVF  A +N R +S+ +   E    + SQ++  +  FLNKA +VPN 
Sbjct: 245 RQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPND 304

Query: 308 SNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSGFII 367
           S++  + C  R+VE+A A++R+ PVW+T L + I FAQ  TFFTKQG TM+RTI  G  I
Sbjct: 305 SDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEI 364

Query: 368 PAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLVATM 427
           P A++   I +SIV+F+ IYD V VPI R+ T    GITTL+RIGTG+V++ ++M+VA +
Sbjct: 365 PPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAAL 424

Query: 428 VERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPTDLK 487
           VE KRL  A+E+GL+D+P+ T+PMS WWL PQY+L GLA+V TLVG+QEFFY Q PT+L+
Sbjct: 425 VESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELR 484

Query: 488 SMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGLSVL 547
           S+GLA Y S +G+GS+LSSLL+  ID ATG +  NSWF++NLN+AHLDYFY LL+ +S +
Sbjct: 485 SLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAV 544

Query: 548 ALVAFLFVSNSHVHSR 562
               FLF+S S+++ R
Sbjct: 545 GFFTFLFISKSYIYRR 557

BLAST of Tan0020075 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 3.4e-159
Identity = 292/566 (51.59%), Postives = 399/566 (70.49%), Query Frame = 0

Query: 3   LSVSLSDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLIT 62
           ++++  ++ L+ D+V  +VD +G P  +SS+GGWRSA ++IGVEV ERFAY+GIG+NLIT
Sbjct: 1   MAIAEEEAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLIT 60

Query: 63  YLTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLS 122
           YLTGPLGQS ATAA +VN WSG + +  +LGAF+AD++ GRYRTI+ +S IY+LGL  L+
Sbjct: 61  YLTGPLGQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLT 120

Query: 123 FSALLPLC-----RNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDA 182
            S++L L      R  N  +S     +  + FF SLYL+ IGQGGHKPCVQAFGADQFD 
Sbjct: 121 LSSILILMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDV 180

Query: 183 QHPQEAKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVF 242
             P+E  S+ SFFNWWF  + AG  ++I+VV Y ++N++W+LGFGIPC+ MV+A  +F+F
Sbjct: 181 GDPKERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLF 240

Query: 243 GTNTYRY--SVKKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRF 302
           G  TYRY    ++  N+ F RIGRVF+ A +N +   T     E G+    ++  GQ  F
Sbjct: 241 GRKTYRYPRGDREGKNNAFARIGRVFLVAFKNRKLKLTHSGQLEVGS---YKKCKGQLEF 300

Query: 303 LNKACIVPNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 362
           L KA ++P         CS R+VE+A A++R+ P+W+T +V  I +AQ +TFFTKQG T+
Sbjct: 301 LAKA-LLPGEGGVE--PCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTV 360

Query: 363 DRTILSGFIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVI 422
           DR IL GF IP A+  + I LSI I +  Y+ VF+P+AR  T   SGIT LQRIG G+V+
Sbjct: 361 DRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVL 420

Query: 423 SVISMLVATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEF 482
           S ++M+VA +VE KRL  A+EHGLVDRP+ TIPMS WW VPQY+L G+ +VF+LVG QEF
Sbjct: 421 SSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEF 480

Query: 483 FYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYF 542
           FYDQ PT+L+S+GLA   S +G+ S LS  L++ I+ ATG NG +SWF+ NLN+AH+DYF
Sbjct: 481 FYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYF 540

Query: 543 YLLLSGLSVLALVAFLFVSNSHVHSR 562
           Y LL+  + +  +AFL +S  +V+ R
Sbjct: 541 YWLLAAFTAIGFLAFLLLSRLYVYRR 560

BLAST of Tan0020075 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 7.6e-159
Identity = 290/569 (50.97%), Postives = 396/569 (69.60%), Query Frame = 0

Query: 3   LSVSLSDSP----LLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGT 62
           +S S+ D+     +  + V+ +VDF+G P +RSSSG W+S+ F +  EVAE+FAY+GI +
Sbjct: 1   MSTSIGDNETGAIVSNENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIAS 60

Query: 63  NLITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGL 122
           NLITY T  LG+S A AA +VN+W G +    L+   +ADSF GR+RTIL +S+ Y++GL
Sbjct: 61  NLITYFTEALGESTAVAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGL 120

Query: 123 AFLSFSALLPLCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQ 182
             L+FSA +P   N  E       Q +++ FF +LYLI +G+GG K C++AFGADQFD Q
Sbjct: 121 GLLTFSATIPSLCNDQETRESCVSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQ 180

Query: 183 HPQEAKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFG 242
            P E+K+KSS+FNW +F I  G     LV  Y +ENLSW+LG+ IPC+ M++A  +F+ G
Sbjct: 181 DPNESKAKSSYFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLG 240

Query: 243 TNTYRYSV-------KKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAG 302
             TYR+S        KK+DN PFVRIGRVFV+A RN R + +      D       ++  
Sbjct: 241 IKTYRFSTGGEGRQGKKHDN-PFVRIGRVFVAAARNRRQTPS------DTCLLLPNESTK 300

Query: 303 QFRFLNKACIVPNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQ 362
           +FRFL++A I           C   EVEEAKAVL + P+W+  LVFGIVFAQ  TFFTKQ
Sbjct: 301 KFRFLDRAVI----------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQ 360

Query: 363 GATMDRTILSGFIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGT 422
           G+TMDR+I S   +PAA +  FI L+I++FI IYD +FVPIAR+ T   +GITTLQRI T
Sbjct: 361 GSTMDRSISSTLQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRIST 420

Query: 423 GLVISVISMLVATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVG 482
           G+ +S+ISM++A +VE KRL+ A +HGLVD P+ T+PMS  WL+PQYIL+G+++VFT+VG
Sbjct: 421 GIFLSIISMVIAALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVG 480

Query: 483 LQEFFYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAH 542
           LQEFFY + P  L+SMGLA Y S++G+G+ LSS +VS I+EAT  +GQ SWFSNNLN+AH
Sbjct: 481 LQEFFYGEVPPQLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAH 540

Query: 543 LDYFYLLLSGLSVLALVAFLFVSNSHVHS 561
           LDYFY LL+ LS LA +  ++ + S++++
Sbjct: 541 LDYFYWLLACLSSLAFIFTVYFAKSYLYN 552

BLAST of Tan0020075 vs. NCBI nr
Match: XP_023541977.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1002.7 bits (2591), Expect = 1.3e-288
Identity = 515/566 (90.99%), Postives = 532/566 (93.99%), Query Frame = 0

Query: 1   MDLSVSLSDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNL 60
           MDLS+S SDSPLL DTVDGAVDFQG PVLRSSSGGWRSASFMIGVEVAERFAYYGIG NL
Sbjct: 41  MDLSLSASDSPLLNDTVDGAVDFQGHPVLRSSSGGWRSASFMIGVEVAERFAYYGIGANL 100

Query: 61  ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAF 120
           ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAF+ADSFFGRYRTILFSSAIYVLGLA 
Sbjct: 101 ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLAL 160

Query: 121 LSFSALLP-----LCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQF 180
           LSFSALLP     LC+N NEISSCST QFQLV FFVSLYLIG+GQGGHKPCVQAFGADQF
Sbjct: 161 LSFSALLPTTTSLLCQNSNEISSCSTAQFQLVLFFVSLYLIGVGQGGHKPCVQAFGADQF 220

Query: 181 DAQHPQEAKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVF 240
           DAQHPQEAKSKSSFFNWWFFG+CAG+FVAILVVTYTEENLSWSLGFGIP IMMVIAS+VF
Sbjct: 221 DAQHPQEAKSKSSFFNWWFFGVCAGSFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVF 280

Query: 241 VFGTNTYRYSVKKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRF 300
           VFG NTYRYSVKKYD SPFVRIGRVFVSA RNWRASST IF QE+ ATD SQQ AGQFRF
Sbjct: 281 VFGKNTYRYSVKKYDKSPFVRIGRVFVSAARNWRASSTFIFYQEESATDLSQQKAGQFRF 340

Query: 301 LNKACIVPNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 360
           LNKACIVPNHSNQ GMVCS+ EVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM
Sbjct: 341 LNKACIVPNHSNQCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 400

Query: 361 DRTILSGFIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVI 420
           DR+ILSGFI PAAAIDSF+PLSIVIFITIYD VFV IARAFTGIQSGITTLQRIGTGLVI
Sbjct: 401 DRSILSGFITPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGIQSGITTLQRIGTGLVI 460

Query: 421 SVISMLVATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEF 480
           S ISMLVA+MVERKRLRIAEE GLVDRP+ITIPMSFWWLVPQY LYGLAEVFTLVGLQEF
Sbjct: 461 SAISMLVASMVERKRLRIAEEQGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQEF 520

Query: 481 FYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYF 540
           FYDQCP+DLKSMGLAFYTSVLGMGSILSSLLVS IDEATGANGQNSWFS+NLNKAHLDYF
Sbjct: 521 FYDQCPSDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGANGQNSWFSDNLNKAHLDYF 580

Query: 541 YLLLSGLSVLALVAFLFVSNSHVHSR 562
           YLLLSGLS+LA V FLFVS SHV+SR
Sbjct: 581 YLLLSGLSLLAFVGFLFVSTSHVYSR 606

BLAST of Tan0020075 vs. NCBI nr
Match: XP_022954701.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata] >XP_022954702.1 protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.2e-288
Identity = 514/566 (90.81%), Postives = 531/566 (93.82%), Query Frame = 0

Query: 1   MDLSVSLSDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNL 60
           MDLSVS SDSPLL DTVDGAVDFQG PVLRSSSGGWRSASFMIGVEVAERFAYYGIG NL
Sbjct: 1   MDLSVSASDSPLLNDTVDGAVDFQGHPVLRSSSGGWRSASFMIGVEVAERFAYYGIGANL 60

Query: 61  ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAF 120
           ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAF+ADSFFGRYRTILFSSAIYVLGLA 
Sbjct: 61  ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLAL 120

Query: 121 LSFSALLP-----LCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQF 180
           LSFSALLP     LC+N NEISSCSTPQFQL+ FFVSLYLIG+GQGGHKPCVQAFGADQF
Sbjct: 121 LSFSALLPTTTSLLCQNSNEISSCSTPQFQLMLFFVSLYLIGVGQGGHKPCVQAFGADQF 180

Query: 181 DAQHPQEAKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVF 240
           DAQHPQEAKSKSSFFNWWFFG+CAGTFVAILVVTYTEENLSWSLGFGIP IMMVIAS+VF
Sbjct: 181 DAQHPQEAKSKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVF 240

Query: 241 VFGTNTYRYSVKKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRF 300
           VFG NTYRYSVKKYD SPFVRIGRVFVSA RNWRASST IF QE+ A D SQQ AGQFRF
Sbjct: 241 VFGKNTYRYSVKKYDKSPFVRIGRVFVSAARNWRASSTFIFGQEESAIDLSQQKAGQFRF 300

Query: 301 LNKACIVPNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 360
           LNKACIVPNHSN  GMVCS+ EVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM
Sbjct: 301 LNKACIVPNHSNLCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 360

Query: 361 DRTILSGFIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVI 420
           DR+ILSGFIIPAAAIDSF+PLSIVIFITIYD VFV IARAFTG+QSGITTLQRIGTGLVI
Sbjct: 361 DRSILSGFIIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGVQSGITTLQRIGTGLVI 420

Query: 421 SVISMLVATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEF 480
           S ISMLVA+MVERKRLRIAEE GLVDRP+ITIPMSFWWLVPQY LYGLAEVFTLVGLQEF
Sbjct: 421 SAISMLVASMVERKRLRIAEEQGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQEF 480

Query: 481 FYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYF 540
           FYDQCP+DLKSMGLAFYTSVLGMGSILSSLLVS IDEATGANGQNSWFS+NLNKAHLDYF
Sbjct: 481 FYDQCPSDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGANGQNSWFSDNLNKAHLDYF 540

Query: 541 YLLLSGLSVLALVAFLFVSNSHVHSR 562
           YLLLSGLS+LA V FLFVS  HV+SR
Sbjct: 541 YLLLSGLSLLAFVGFLFVSTWHVYSR 566

BLAST of Tan0020075 vs. NCBI nr
Match: XP_022994214.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima])

HSP 1 Score: 990.7 bits (2560), Expect = 5.1e-285
Identity = 509/557 (91.38%), Postives = 524/557 (94.08%), Query Frame = 0

Query: 1   MDLSVSLSDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNL 60
           MDLSVS SDSPLL DTVDG VDFQG PVLRSS+GGWRSASFMIGVEVAERFAYYGIG NL
Sbjct: 1   MDLSVSASDSPLLNDTVDGTVDFQGHPVLRSSAGGWRSASFMIGVEVAERFAYYGIGANL 60

Query: 61  ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAF 120
           ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAF+ADSFFGRYRTILFSSAIYVLGLA 
Sbjct: 61  ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLAL 120

Query: 121 LSFSALLP-----LCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQF 180
           LSFSALLP     LC+N NEISSCSTPQFQLV FFVSLYLIG+GQGGHKPCVQAFGADQF
Sbjct: 121 LSFSALLPTTTSLLCQNSNEISSCSTPQFQLVLFFVSLYLIGVGQGGHKPCVQAFGADQF 180

Query: 181 DAQHPQEAKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVF 240
           DAQHPQEAKSKSSFFNWWFFG+CAGTFVAILVVTYTEENLSWSLGFGIP IMMVIAS+VF
Sbjct: 181 DAQHPQEAKSKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVF 240

Query: 241 VFGTNTYRYSVKKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRF 300
           VFG  TYRYSVKKYD SPFVRIGRVFVSA RNWRASST IFDQE+ ATD SQQ AGQFRF
Sbjct: 241 VFGKYTYRYSVKKYDKSPFVRIGRVFVSAARNWRASSTFIFDQEESATDLSQQKAGQFRF 300

Query: 301 LNKACIVPNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 360
           LNKACIVPNHSNQ GMVCS+ EVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM
Sbjct: 301 LNKACIVPNHSNQCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 360

Query: 361 DRTILSGFIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVI 420
           DR+ILSGFIIPAAAIDSF+PLSIVIFITIYD VFV IARAFTGIQSGITTLQRIGTGLVI
Sbjct: 361 DRSILSGFIIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGIQSGITTLQRIGTGLVI 420

Query: 421 SVISMLVATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEF 480
           S ISMLVA+MVERKRLRIAEE GLVDRP+ITIPMSFWWLVPQY LYGLAEVFTLVGLQEF
Sbjct: 421 SAISMLVASMVERKRLRIAEETGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQEF 480

Query: 481 FYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYF 540
           FYDQCP+DLKSMGLAFYTSVLG GSILSSLLVS IDEATGANGQNSWFS+NLNKAHLDYF
Sbjct: 481 FYDQCPSDLKSMGLAFYTSVLGTGSILSSLLVSVIDEATGANGQNSWFSDNLNKAHLDYF 540

Query: 541 YLLLSGLSVLALVAFLF 553
           YLLLSGLS+LA V FLF
Sbjct: 541 YLLLSGLSLLAFVGFLF 557

BLAST of Tan0020075 vs. NCBI nr
Match: XP_038891005.1 (protein NRT1/ PTR FAMILY 5.10-like [Benincasa hispida])

HSP 1 Score: 932.6 bits (2409), Expect = 1.6e-267
Identity = 468/552 (84.78%), Postives = 509/552 (92.21%), Query Frame = 0

Query: 10  SPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITYLTGPLG 69
           +PLL  TV+GAVDF+GQPVLR SSGGWRSASFMIGVEVAERFAYYGIG+NLITYLTGPLG
Sbjct: 5   TPLLRQTVEGAVDFKGQPVLRFSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64

Query: 70  QSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSFSALLPL 129
           QSVATAAE+VNIWSGISML TLLGAFLADSFFGRYRTILFSSAIYVLGL+ LSFSA+LP 
Sbjct: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAMLPT 124

Query: 130 CRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQEAKSKSSF 189
             +P         QFQL+ FFVSLYLIGIGQGGHKPCVQAFGADQFDA  PQEAKSKSSF
Sbjct: 125 TTSP------QNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPQEAKSKSSF 184

Query: 190 FNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTYRYSVKKY 249
           FNWWFFG+CAGTFVAIL+VTYTEENLSWSLGFGIPCIMM+IAS +F+ GTNTYRY++K Y
Sbjct: 185 FNWWFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLLGTNTYRYTIKTY 244

Query: 250 DNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIVPNHSNQS 309
           D +PF+RIGRVFVSA+RNWRASST+IFD+E    D SQQNAGQFRFLNKACIVP  SN+ 
Sbjct: 245 DKTPFLRIGRVFVSAIRNWRASSTVIFDEEGNGRDLSQQNAGQFRFLNKACIVPKDSNKC 304

Query: 310 GMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSGFIIPAAA 369
           GM+CSV EVEEAKAVLRIFP+W+TVLVF IVF+QDSTFFTKQGATMDR+ILSGFIIPAAA
Sbjct: 305 GMMCSVSEVEEAKAVLRIFPIWITVLVFAIVFSQDSTFFTKQGATMDRSILSGFIIPAAA 364

Query: 370 IDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLVATMVERK 429
           +D+F+PLSIVIF+TIYDL+FVPIARAFTGIQSGITTLQRIGTGLV+S ISMLVATMVERK
Sbjct: 365 LDAFVPLSIVIFVTIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISMLVATMVERK 424

Query: 430 RLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPTDLKSMGL 489
           RLRIA++HGLVD+P+ITIP+SFWWLVPQY L+GLAEVFTLVGLQEFFYDQ PTDLKSMGL
Sbjct: 425 RLRIADKHGLVDKPDITIPISFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGL 484

Query: 490 AFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGLSVLALVA 549
           AFYTSVLGMGSILSSLLVS IDE TG + QNSWFSNNLNKAHLDYFYLLLSGLSV+A VA
Sbjct: 485 AFYTSVLGMGSILSSLLVSVIDEVTGGSEQNSWFSNNLNKAHLDYFYLLLSGLSVVAFVA 544

Query: 550 FLFVSNSHVHSR 562
           FLFVS SHVHSR
Sbjct: 545 FLFVSKSHVHSR 550

BLAST of Tan0020075 vs. NCBI nr
Match: XP_008459680.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo])

HSP 1 Score: 922.2 bits (2382), Expect = 2.2e-264
Identity = 467/552 (84.60%), Postives = 503/552 (91.12%), Query Frame = 0

Query: 10  SPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITYLTGPLG 69
           +PLL+ TV+ AVDF+GQP LR  SGGWRSASFMIGVEVAERFAYYGIG+NLITYLTGPLG
Sbjct: 5   TPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64

Query: 70  QSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSFSALLPL 129
           QSVATAAE+VNIWSGISML TLLGAFLADSFFGRYRTILFSSAIYVLGL+ LSFSA+LP 
Sbjct: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAMLPT 124

Query: 130 CRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQEAKSKSSF 189
             + N        QFQL+FFFVSLYLIGIGQGGHKPCVQAFGADQFDA HPQEAKSKSSF
Sbjct: 125 TSSQNS-------QFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSF 184

Query: 190 FNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTYRYSVKKY 249
           FNWWFFG+CAGTFVAIL+VTYTEENLSWSLGFGIPCIMMVIAS +F+FGTNTYRYS+K Y
Sbjct: 185 FNWWFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGTNTYRYSIKMY 244

Query: 250 DNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIVPNHSNQS 309
             +PF+RIGRVFVSA+RN RASST+ FD+E    D S+QN GQF FLNKACIVP  SN+ 
Sbjct: 245 AKTPFLRIGRVFVSAIRNCRASSTVTFDEEGDGPDLSEQNTGQFSFLNKACIVPKDSNKH 304

Query: 310 GMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSGFIIPAAA 369
           G++CS  EVEEAKAVLRIFPVW+TVLVF IVFAQDSTFFTKQGAT+DR+ILSGFIIPAAA
Sbjct: 305 GVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSILSGFIIPAAA 364

Query: 370 IDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLVATMVERK 429
           +DSF+PLSIVIFITIYDL+FVPIARAFTGIQSGITTLQRIGTGLV+S ISMLVATMVERK
Sbjct: 365 LDSFVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISMLVATMVERK 424

Query: 430 RLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPTDLKSMGL 489
           RLRIA+EHGLVDRP+ITIPMSFWWLVPQY L+GLAEVFTLVGLQEFFYDQ PTDLKSMGL
Sbjct: 425 RLRIADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGL 484

Query: 490 AFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGLSVLALVA 549
           AFYTSVLGMGSILSSLLVS IDE TG + QNSWFSNNLNKAHLDYFYLLLS LSV+A VA
Sbjct: 485 AFYTSVLGMGSILSSLLVSIIDEVTGGSEQNSWFSNNLNKAHLDYFYLLLSALSVVAFVA 544

Query: 550 FLFVSNSHVHSR 562
           FLFVS SHVHSR
Sbjct: 545 FLFVSKSHVHSR 549

BLAST of Tan0020075 vs. ExPASy TrEMBL
Match: A0A6J1GRM0 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111456880 PE=3 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.1e-288
Identity = 514/566 (90.81%), Postives = 531/566 (93.82%), Query Frame = 0

Query: 1   MDLSVSLSDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNL 60
           MDLSVS SDSPLL DTVDGAVDFQG PVLRSSSGGWRSASFMIGVEVAERFAYYGIG NL
Sbjct: 1   MDLSVSASDSPLLNDTVDGAVDFQGHPVLRSSSGGWRSASFMIGVEVAERFAYYGIGANL 60

Query: 61  ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAF 120
           ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAF+ADSFFGRYRTILFSSAIYVLGLA 
Sbjct: 61  ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLAL 120

Query: 121 LSFSALLP-----LCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQF 180
           LSFSALLP     LC+N NEISSCSTPQFQL+ FFVSLYLIG+GQGGHKPCVQAFGADQF
Sbjct: 121 LSFSALLPTTTSLLCQNSNEISSCSTPQFQLMLFFVSLYLIGVGQGGHKPCVQAFGADQF 180

Query: 181 DAQHPQEAKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVF 240
           DAQHPQEAKSKSSFFNWWFFG+CAGTFVAILVVTYTEENLSWSLGFGIP IMMVIAS+VF
Sbjct: 181 DAQHPQEAKSKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVF 240

Query: 241 VFGTNTYRYSVKKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRF 300
           VFG NTYRYSVKKYD SPFVRIGRVFVSA RNWRASST IF QE+ A D SQQ AGQFRF
Sbjct: 241 VFGKNTYRYSVKKYDKSPFVRIGRVFVSAARNWRASSTFIFGQEESAIDLSQQKAGQFRF 300

Query: 301 LNKACIVPNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 360
           LNKACIVPNHSN  GMVCS+ EVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM
Sbjct: 301 LNKACIVPNHSNLCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 360

Query: 361 DRTILSGFIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVI 420
           DR+ILSGFIIPAAAIDSF+PLSIVIFITIYD VFV IARAFTG+QSGITTLQRIGTGLVI
Sbjct: 361 DRSILSGFIIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGVQSGITTLQRIGTGLVI 420

Query: 421 SVISMLVATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEF 480
           S ISMLVA+MVERKRLRIAEE GLVDRP+ITIPMSFWWLVPQY LYGLAEVFTLVGLQEF
Sbjct: 421 SAISMLVASMVERKRLRIAEEQGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQEF 480

Query: 481 FYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYF 540
           FYDQCP+DLKSMGLAFYTSVLGMGSILSSLLVS IDEATGANGQNSWFS+NLNKAHLDYF
Sbjct: 481 FYDQCPSDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGANGQNSWFSDNLNKAHLDYF 540

Query: 541 YLLLSGLSVLALVAFLFVSNSHVHSR 562
           YLLLSGLS+LA V FLFVS  HV+SR
Sbjct: 541 YLLLSGLSLLAFVGFLFVSTWHVYSR 566

BLAST of Tan0020075 vs. ExPASy TrEMBL
Match: A0A6J1K2A2 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111490020 PE=3 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.5e-285
Identity = 509/557 (91.38%), Postives = 524/557 (94.08%), Query Frame = 0

Query: 1   MDLSVSLSDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNL 60
           MDLSVS SDSPLL DTVDG VDFQG PVLRSS+GGWRSASFMIGVEVAERFAYYGIG NL
Sbjct: 1   MDLSVSASDSPLLNDTVDGTVDFQGHPVLRSSAGGWRSASFMIGVEVAERFAYYGIGANL 60

Query: 61  ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAF 120
           ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAF+ADSFFGRYRTILFSSAIYVLGLA 
Sbjct: 61  ITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLAL 120

Query: 121 LSFSALLP-----LCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQF 180
           LSFSALLP     LC+N NEISSCSTPQFQLV FFVSLYLIG+GQGGHKPCVQAFGADQF
Sbjct: 121 LSFSALLPTTTSLLCQNSNEISSCSTPQFQLVLFFVSLYLIGVGQGGHKPCVQAFGADQF 180

Query: 181 DAQHPQEAKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVF 240
           DAQHPQEAKSKSSFFNWWFFG+CAGTFVAILVVTYTEENLSWSLGFGIP IMMVIAS+VF
Sbjct: 181 DAQHPQEAKSKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVF 240

Query: 241 VFGTNTYRYSVKKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRF 300
           VFG  TYRYSVKKYD SPFVRIGRVFVSA RNWRASST IFDQE+ ATD SQQ AGQFRF
Sbjct: 241 VFGKYTYRYSVKKYDKSPFVRIGRVFVSAARNWRASSTFIFDQEESATDLSQQKAGQFRF 300

Query: 301 LNKACIVPNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 360
           LNKACIVPNHSNQ GMVCS+ EVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM
Sbjct: 301 LNKACIVPNHSNQCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 360

Query: 361 DRTILSGFIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVI 420
           DR+ILSGFIIPAAAIDSF+PLSIVIFITIYD VFV IARAFTGIQSGITTLQRIGTGLVI
Sbjct: 361 DRSILSGFIIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGIQSGITTLQRIGTGLVI 420

Query: 421 SVISMLVATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEF 480
           S ISMLVA+MVERKRLRIAEE GLVDRP+ITIPMSFWWLVPQY LYGLAEVFTLVGLQEF
Sbjct: 421 SAISMLVASMVERKRLRIAEETGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQEF 480

Query: 481 FYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYF 540
           FYDQCP+DLKSMGLAFYTSVLG GSILSSLLVS IDEATGANGQNSWFS+NLNKAHLDYF
Sbjct: 481 FYDQCPSDLKSMGLAFYTSVLGTGSILSSLLVSVIDEATGANGQNSWFSDNLNKAHLDYF 540

Query: 541 YLLLSGLSVLALVAFLF 553
           YLLLSGLS+LA V FLF
Sbjct: 541 YLLLSGLSLLAFVGFLF 557

BLAST of Tan0020075 vs. ExPASy TrEMBL
Match: A0A1S3CAV1 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103498725 PE=3 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 1.1e-264
Identity = 467/552 (84.60%), Postives = 503/552 (91.12%), Query Frame = 0

Query: 10  SPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITYLTGPLG 69
           +PLL+ TV+ AVDF+GQP LR  SGGWRSASFMIGVEVAERFAYYGIG+NLITYLTGPLG
Sbjct: 5   TPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 64

Query: 70  QSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSFSALLPL 129
           QSVATAAE+VNIWSGISML TLLGAFLADSFFGRYRTILFSSAIYVLGL+ LSFSA+LP 
Sbjct: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAMLPT 124

Query: 130 CRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQEAKSKSSF 189
             + N        QFQL+FFFVSLYLIGIGQGGHKPCVQAFGADQFDA HPQEAKSKSSF
Sbjct: 125 TSSQNS-------QFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSF 184

Query: 190 FNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTYRYSVKKY 249
           FNWWFFG+CAGTFVAIL+VTYTEENLSWSLGFGIPCIMMVIAS +F+FGTNTYRYS+K Y
Sbjct: 185 FNWWFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGTNTYRYSIKMY 244

Query: 250 DNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIVPNHSNQS 309
             +PF+RIGRVFVSA+RN RASST+ FD+E    D S+QN GQF FLNKACIVP  SN+ 
Sbjct: 245 AKTPFLRIGRVFVSAIRNCRASSTVTFDEEGDGPDLSEQNTGQFSFLNKACIVPKDSNKH 304

Query: 310 GMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSGFIIPAAA 369
           G++CS  EVEEAKAVLRIFPVW+TVLVF IVFAQDSTFFTKQGAT+DR+ILSGFIIPAAA
Sbjct: 305 GVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSILSGFIIPAAA 364

Query: 370 IDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLVATMVERK 429
           +DSF+PLSIVIFITIYDL+FVPIARAFTGIQSGITTLQRIGTGLV+S ISMLVATMVERK
Sbjct: 365 LDSFVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISMLVATMVERK 424

Query: 430 RLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPTDLKSMGL 489
           RLRIA+EHGLVDRP+ITIPMSFWWLVPQY L+GLAEVFTLVGLQEFFYDQ PTDLKSMGL
Sbjct: 425 RLRIADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGL 484

Query: 490 AFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGLSVLALVA 549
           AFYTSVLGMGSILSSLLVS IDE TG + QNSWFSNNLNKAHLDYFYLLLS LSV+A VA
Sbjct: 485 AFYTSVLGMGSILSSLLVSIIDEVTGGSEQNSWFSNNLNKAHLDYFYLLLSALSVVAFVA 544

Query: 550 FLFVSNSHVHSR 562
           FLFVS SHVHSR
Sbjct: 545 FLFVSKSHVHSR 549

BLAST of Tan0020075 vs. ExPASy TrEMBL
Match: A0A6J1DNR2 (protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC111022354 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.6e-258
Identity = 450/551 (81.67%), Postives = 498/551 (90.38%), Query Frame = 0

Query: 10  SPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITYLTGPLG 69
           +PLL+DTVDGAVDF+GQPVLRSSSGGWRSASF+IGVEVAERFAYYGIG+NLITYLTGPLG
Sbjct: 11  TPLLSDTVDGAVDFKGQPVLRSSSGGWRSASFIIGVEVAERFAYYGIGSNLITYLTGPLG 70

Query: 70  QSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSFSALLPL 129
            SVATAAES+N+WSG SML TLLGAF+ADSFFG YRTIL SSAIYVLGL  LSFSALLP 
Sbjct: 71  LSVATAAESLNVWSGTSMLLTLLGAFVADSFFGPYRTILLSSAIYVLGLGLLSFSALLPT 130

Query: 130 CRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQEAKSKSSF 189
                  SS S  + Q++ FFVSLYLIGIGQGGHKPCVQAFGADQFD +HP+E KSKSSF
Sbjct: 131 TN-----SSVSHNKLQIILFFVSLYLIGIGQGGHKPCVQAFGADQFDGRHPEEVKSKSSF 190

Query: 190 FNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTYRYSVKKY 249
           FNWWFFG+CAGTFVA+LVVTYTEEN+SWSLGFGIPC+ MVIA +VF+FGTNTYRYSVK+Y
Sbjct: 191 FNWWFFGLCAGTFVAMLVVTYTEENVSWSLGFGIPCLAMVIALVVFLFGTNTYRYSVKEY 250

Query: 250 DNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIVPNHSNQS 309
             SPFVRIGRVFVSA+RNWRAS T+ FD+EDG    S QNAGQFRFLNKAC VPNHS+Q 
Sbjct: 251 GKSPFVRIGRVFVSAIRNWRASPTVFFDEEDGGRVLSHQNAGQFRFLNKACAVPNHSSQC 310

Query: 310 GMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSGFIIPAAA 369
           GM CS+ EVEEAKAVLRI P+W+T+LVF IVFAQD+TFFTKQGATM+R+I SGFIIPAAA
Sbjct: 311 GMACSISEVEEAKAVLRILPIWITILVFAIVFAQDTTFFTKQGATMNRSIFSGFIIPAAA 370

Query: 370 IDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLVATMVERK 429
           ++S +PLSIVIFI++YDLVFVP+ARAFTGIQSGIT LQRIGTGLVIS ISM+VATMVERK
Sbjct: 371 LESLVPLSIVIFISVYDLVFVPVARAFTGIQSGITPLQRIGTGLVISAISMVVATMVERK 430

Query: 430 RLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPTDLKSMGL 489
           RLR AEEHG+VD P+ITIPMSFWWLVPQY LYGLA+VFT+VGLQEFFY+QCP DLKSMGL
Sbjct: 431 RLRSAEEHGVVDSPDITIPMSFWWLVPQYSLYGLADVFTVVGLQEFFYNQCPADLKSMGL 490

Query: 490 AFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGLSVLALVA 549
           A YTSVLGMGSILSS+LVS IDEA+G NG NSWFS+NLNKAHLDYFYLLLSGLSV+A VA
Sbjct: 491 ALYTSVLGMGSILSSVLVSVIDEASGGNGHNSWFSDNLNKAHLDYFYLLLSGLSVVAFVA 550

Query: 550 FLFVSNSHVHS 561
           FLFVS SHV+S
Sbjct: 551 FLFVSKSHVYS 556

BLAST of Tan0020075 vs. ExPASy TrEMBL
Match: A0A5A7T8K1 (Proton-dependent oligopeptide transporter family OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00050 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.9e-246
Identity = 442/551 (80.22%), Postives = 477/551 (86.57%), Query Frame = 0

Query: 10  SPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITYLTGPLG 69
           +PLL+ TV+ AVDF+GQP LR  SGGWRSASFMIGVEVAERFAYYGIG+NLITYLTGPLG
Sbjct: 106 TPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTGPLG 165

Query: 70  QSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSFSALLPL 129
           QSVATAAE+VNIWSGISML TLLGAFLADSFFGRYRTILFSSAIYVLGL+ LSFSA+LP 
Sbjct: 166 QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSAMLPT 225

Query: 130 CRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQEAKSKSSF 189
             + N        QFQL+FFFVSLYLIGIGQGGHKPCVQAFGADQFDA HPQEAKSKSSF
Sbjct: 226 TSSQNS-------QFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSF 285

Query: 190 FNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTYRYSVKKY 249
           FNWWFFG+CAGTFVAIL+VTYTEENLSWSLGFGIPCIMMVIAS +F+FGTNTYRYS+K Y
Sbjct: 286 FNWWFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGTNTYRYSIKMY 345

Query: 250 DNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIVPNHSNQS 309
             +PF+RIGRVFVSA+RN RASST+ FD+E    D S+QN GQF FLNKACIVP  SN+ 
Sbjct: 346 AKTPFLRIGRVFVSAIRNCRASSTVTFDEEGDGPDLSEQNTGQFSFLNKACIVPKDSNKH 405

Query: 310 GMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSGFIIPAAA 369
           G++CS  E                          DSTFFTKQGAT+DR+ILSGFIIPAAA
Sbjct: 406 GVMCSASE--------------------------DSTFFTKQGATIDRSILSGFIIPAAA 465

Query: 370 IDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLVATMVERK 429
           +DSF+PLSIVIFITIYDL+FVPIARAFTGIQSGITTLQRIGTGLV+S ISMLVATMVERK
Sbjct: 466 LDSFVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISMLVATMVERK 525

Query: 430 RLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPTDLKSMGL 489
           RLRIA+EHGLVDRP+ITIPMSFWWLVPQY L+GLAEVFTLVGLQEFFYDQ PTDLKSMGL
Sbjct: 526 RLRIADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVPTDLKSMGL 585

Query: 490 AFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGLSVLALVA 549
           AFYTSVLGMGSILSSLLVS IDE TG + QNSWFSNNLNKAHLDYFYLLLS LSV+A VA
Sbjct: 586 AFYTSVLGMGSILSSLLVSIIDEVTGGSEQNSWFSNNLNKAHLDYFYLLLSALSVVAFVA 623

Query: 550 FLFVSNSHVHS 561
           FLFVS SHVHS
Sbjct: 646 FLFVSKSHVHS 623

BLAST of Tan0020075 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 651.4 bits (1679), Expect = 6.8e-187
Identity = 322/559 (57.60%), Postives = 427/559 (76.39%), Query Frame = 0

Query: 4   SVSLSDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITY 63
           +V  + +PLL  T    VD++ +P ++SSSGGWRSA F+IGVEVAERFAYYGI +NLITY
Sbjct: 6   AVDEAGTPLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITY 65

Query: 64  LTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSF 123
           LTGPLGQS A AA +VN WSG + L  LLGAF+ADSF GR+RTIL +SA+Y++GL  L+ 
Sbjct: 66  LTGPLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTL 125

Query: 124 SALLPL-CRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQE 183
           SA++P  C+  N +SSCS P+FQ++ FF +LYL+ + QGGHKPCVQAFGADQFD + P+E
Sbjct: 126 SAMIPSDCKVSNLLSSCS-PRFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEE 185

Query: 184 AKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTY 243
            K+KSSFFNWW+FG+C GT   + V+ Y ++NLSW+LGFGIPCI MV+A +V + GT TY
Sbjct: 186 CKAKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTY 245

Query: 244 RYSVKKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIV 303
           R+S+++ D SPFVRIG V+V+AV+NW  S+  +   E+     S  ++ QF FLNKA + 
Sbjct: 246 RFSIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVA 305

Query: 304 PNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSG 363
            N S      CS+ E+EEAK+VLR+ P+W+T LV+ +VFAQ  TFFTKQGATM+R+I  G
Sbjct: 306 KNGS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPG 365

Query: 364 FIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLV 423
           + I  A + SFI LSIVIFI IYD V +PIAR+FT    GIT LQRIGTG+ +S ++M+V
Sbjct: 366 YKISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVV 425

Query: 424 ATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPT 483
           A +VE KRL+ A ++GLVD P+ T+PMS WWLVPQY+L+G+ +VF +VGLQEFFYDQ P 
Sbjct: 426 AALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPN 485

Query: 484 DLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGL 543
           +L+S+GLA Y S+ G+G+ LSS ++S I++AT  +GQ SWF+NNLN+AHLDYFY LL+ L
Sbjct: 486 ELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACL 545

Query: 544 SVLALVAFLFVSNSHVHSR 562
           S + L ++L+V+ S+V  R
Sbjct: 546 SFIGLASYLYVAKSYVSKR 553

BLAST of Tan0020075 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 610.1 bits (1572), Expect = 1.7e-174
Identity = 316/556 (56.83%), Postives = 405/556 (72.84%), Query Frame = 0

Query: 8   SDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITYLTGP 67
           S+  L  + V  AVD +G    RS++G WR+A F+IGVEVAERFAYYGIG+NLI+YLTGP
Sbjct: 5   SEISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGP 64

Query: 68  LGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSFSALL 127
           LG+S A AA +VN WSGI+ L  +LGAF+AD+F GRYRTI+ SS IYVLGLAFL+ SA  
Sbjct: 65  LGESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAF- 124

Query: 128 PLCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQEAKSKS 187
            L  N  E++S ST  F  V FF SLYL+ IGQ GHKPCVQAFGADQFD +  QE   +S
Sbjct: 125 -LIPNTTEVTS-STSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRS 184

Query: 188 SFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTYRYSVK 247
           SFFNWW+  + AG   AILVV Y +E  SW+ GFGIPC+ MVI+ ++FV G   YRYS +
Sbjct: 185 SFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKR 244

Query: 248 KYDN--SPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIVPNH 307
           +++   +PF RIGRVF  A++N R SS+ +   E  A    ++ +    F NKA +VPN 
Sbjct: 245 RHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQS----FFNKALLVPND 304

Query: 308 SNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSGFII 367
           S+Q        +VE+A A++R+ PVW T L + I +AQ  TFFTKQG TMDRTIL G  I
Sbjct: 305 SSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKI 364

Query: 368 PAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLVATM 427
           P A++  FI +SIV+F+ IYD VFVPIAR  T    GITTL+RIGTG+V+S I+M++A +
Sbjct: 365 PPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAAL 424

Query: 428 VERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPTDLK 487
           VE KRL  A+EHGL+D+PE T+PMS WWL+PQY+L GLA+V+TLVG+QEFFY Q PT+L+
Sbjct: 425 VEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELR 484

Query: 488 SMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGLSVL 547
           S+GLA Y S LG+GS+LSSLL+S ID ATG +  NSWF++NLN+AHLDYFY LL+ +S +
Sbjct: 485 SIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAV 544

Query: 548 ALVAFLFVSNSHVHSR 562
               FLF+S S+++ R
Sbjct: 545 GFFTFLFISKSYIYRR 553

BLAST of Tan0020075 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 602.1 bits (1551), Expect = 4.7e-172
Identity = 309/556 (55.58%), Postives = 407/556 (73.20%), Query Frame = 0

Query: 8   SDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLITYLTGP 67
           S + L  + V  AVD +G    RS +G WR+A F+IGVEVAERFA YGIG+NLI+YLTGP
Sbjct: 5   SKTSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGP 64

Query: 68  LGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLSFSALL 127
           LGQS A AA +VN WSGIS +  LLGAF+AD+F GRY TI+ +S IYVLGLAFL+ SA  
Sbjct: 65  LGQSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAF- 124

Query: 128 PLCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQHPQEAKSKS 187
            L  N  E++S S   F    FF SLYL+ IGQ GHKPCVQAFGADQFD ++PQE   +S
Sbjct: 125 -LIPNNTEVTS-SPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDRS 184

Query: 188 SFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFGTNTYRYSV- 247
           SFFNWW+  +CAG  +AILVV Y +EN+SW+LGFGIPC+ MVI+ ++FV G  +YR+S  
Sbjct: 185 SFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSKT 244

Query: 248 -KKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRFLNKACIVPNH 307
            ++ + +PF RIGRVF  A +N R +S+ +   E    + SQ++  +  FLNKA +VPN 
Sbjct: 245 RQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPND 304

Query: 308 SNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATMDRTILSGFII 367
           S++  + C  R+VE+A A++R+ PVW+T L + I FAQ  TFFTKQG TM+RTI  G  I
Sbjct: 305 SDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEI 364

Query: 368 PAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVISVISMLVATM 427
           P A++   I +SIV+F+ IYD V VPI R+ T    GITTL+RIGTG+V++ ++M+VA +
Sbjct: 365 PPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAAL 424

Query: 428 VERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEFFYDQCPTDLK 487
           VE KRL  A+E+GL+D+P+ T+PMS WWL PQY+L GLA+V TLVG+QEFFY Q PT+L+
Sbjct: 425 VESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELR 484

Query: 488 SMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYFYLLLSGLSVL 547
           S+GLA Y S +G+GS+LSSLL+  ID ATG +  NSWF++NLN+AHLDYFY LL+ +S +
Sbjct: 485 SLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAV 544

Query: 548 ALVAFLFVSNSHVHSR 562
               FLF+S S+++ R
Sbjct: 545 GFFTFLFISKSYIYRR 557

BLAST of Tan0020075 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 563.1 bits (1450), Expect = 2.4e-160
Identity = 292/566 (51.59%), Postives = 399/566 (70.49%), Query Frame = 0

Query: 3   LSVSLSDSPLLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGTNLIT 62
           ++++  ++ L+ D+V  +VD +G P  +SS+GGWRSA ++IGVEV ERFAY+GIG+NLIT
Sbjct: 1   MAIAEEEAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLIT 60

Query: 63  YLTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGLAFLS 122
           YLTGPLGQS ATAA +VN WSG + +  +LGAF+AD++ GRYRTI+ +S IY+LGL  L+
Sbjct: 61  YLTGPLGQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLT 120

Query: 123 FSALLPLC-----RNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDA 182
            S++L L      R  N  +S     +  + FF SLYL+ IGQGGHKPCVQAFGADQFD 
Sbjct: 121 LSSILILMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDV 180

Query: 183 QHPQEAKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVF 242
             P+E  S+ SFFNWWF  + AG  ++I+VV Y ++N++W+LGFGIPC+ MV+A  +F+F
Sbjct: 181 GDPKERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLF 240

Query: 243 GTNTYRY--SVKKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAGQFRF 302
           G  TYRY    ++  N+ F RIGRVF+ A +N +   T     E G+    ++  GQ  F
Sbjct: 241 GRKTYRYPRGDREGKNNAFARIGRVFLVAFKNRKLKLTHSGQLEVGS---YKKCKGQLEF 300

Query: 303 LNKACIVPNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGATM 362
           L KA ++P         CS R+VE+A A++R+ P+W+T +V  I +AQ +TFFTKQG T+
Sbjct: 301 LAKA-LLPGEGGVE--PCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTV 360

Query: 363 DRTILSGFIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGTGLVI 422
           DR IL GF IP A+  + I LSI I +  Y+ VF+P+AR  T   SGIT LQRIG G+V+
Sbjct: 361 DRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVL 420

Query: 423 SVISMLVATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVGLQEF 482
           S ++M+VA +VE KRL  A+EHGLVDRP+ TIPMS WW VPQY+L G+ +VF+LVG QEF
Sbjct: 421 SSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEF 480

Query: 483 FYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAHLDYF 542
           FYDQ PT+L+S+GLA   S +G+ S LS  L++ I+ ATG NG +SWF+ NLN+AH+DYF
Sbjct: 481 FYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYF 540

Query: 543 YLLLSGLSVLALVAFLFVSNSHVHSR 562
           Y LL+  + +  +AFL +S  +V+ R
Sbjct: 541 YWLLAAFTAIGFLAFLLLSRLYVYRR 560

BLAST of Tan0020075 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 562.0 bits (1447), Expect = 5.4e-160
Identity = 290/569 (50.97%), Postives = 396/569 (69.60%), Query Frame = 0

Query: 3   LSVSLSDSP----LLTDTVDGAVDFQGQPVLRSSSGGWRSASFMIGVEVAERFAYYGIGT 62
           +S S+ D+     +  + V+ +VDF+G P +RSSSG W+S+ F +  EVAE+FAY+GI +
Sbjct: 1   MSTSIGDNETGAIVSNENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIAS 60

Query: 63  NLITYLTGPLGQSVATAAESVNIWSGISMLFTLLGAFLADSFFGRYRTILFSSAIYVLGL 122
           NLITY T  LG+S A AA +VN+W G +    L+   +ADSF GR+RTIL +S+ Y++GL
Sbjct: 61  NLITYFTEALGESTAVAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGL 120

Query: 123 AFLSFSALLPLCRNPNEISSCSTPQFQLVFFFVSLYLIGIGQGGHKPCVQAFGADQFDAQ 182
             L+FSA +P   N  E       Q +++ FF +LYLI +G+GG K C++AFGADQFD Q
Sbjct: 121 GLLTFSATIPSLCNDQETRESCVSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQ 180

Query: 183 HPQEAKSKSSFFNWWFFGICAGTFVAILVVTYTEENLSWSLGFGIPCIMMVIASLVFVFG 242
            P E+K+KSS+FNW +F I  G     LV  Y +ENLSW+LG+ IPC+ M++A  +F+ G
Sbjct: 181 DPNESKAKSSYFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLG 240

Query: 243 TNTYRYSV-------KKYDNSPFVRIGRVFVSAVRNWRASSTIIFDQEDGATDFSQQNAG 302
             TYR+S        KK+DN PFVRIGRVFV+A RN R + +      D       ++  
Sbjct: 241 IKTYRFSTGGEGRQGKKHDN-PFVRIGRVFVAAARNRRQTPS------DTCLLLPNESTK 300

Query: 303 QFRFLNKACIVPNHSNQSGMVCSVREVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQ 362
           +FRFL++A I           C   EVEEAKAVL + P+W+  LVFGIVFAQ  TFFTKQ
Sbjct: 301 KFRFLDRAVI----------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQ 360

Query: 363 GATMDRTILSGFIIPAAAIDSFIPLSIVIFITIYDLVFVPIARAFTGIQSGITTLQRIGT 422
           G+TMDR+I S   +PAA +  FI L+I++FI IYD +FVPIAR+ T   +GITTLQRI T
Sbjct: 361 GSTMDRSISSTLQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRIST 420

Query: 423 GLVISVISMLVATMVERKRLRIAEEHGLVDRPEITIPMSFWWLVPQYILYGLAEVFTLVG 482
           G+ +S+ISM++A +VE KRL+ A +HGLVD P+ T+PMS  WL+PQYIL+G+++VFT+VG
Sbjct: 421 GIFLSIISMVIAALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVG 480

Query: 483 LQEFFYDQCPTDLKSMGLAFYTSVLGMGSILSSLLVSAIDEATGANGQNSWFSNNLNKAH 542
           LQEFFY + P  L+SMGLA Y S++G+G+ LSS +VS I+EAT  +GQ SWFSNNLN+AH
Sbjct: 481 LQEFFYGEVPPQLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAH 540

Query: 543 LDYFYLLLSGLSVLALVAFLFVSNSHVHS 561
           LDYFY LL+ LS LA +  ++ + S++++
Sbjct: 541 LDYFYWLLACLSSLAFIFTVYFAKSYLYN 552

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WP019.5e-18657.60Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV... [more]
Q8VZE22.4e-17356.83Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV... [more]
Q0WSZ66.6e-17155.58Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV... [more]
Q9SK963.4e-15951.59Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV... [more]
Q9C7U17.6e-15950.97Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV... [more]
Match NameE-valueIdentityDescription
XP_023541977.11.3e-28890.99protein NRT1/ PTR FAMILY 5.10-like [Cucurbita pepo subsp. pepo][more]
XP_022954701.12.2e-28890.81protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata] >XP_022954702.1 protein ... [more]
XP_022994214.15.1e-28591.38protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima][more]
XP_038891005.11.6e-26784.78protein NRT1/ PTR FAMILY 5.10-like [Benincasa hispida][more]
XP_008459680.12.2e-26484.60PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1GRM01.1e-28890.81protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111456880... [more]
A0A6J1K2A22.5e-28591.38protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111490020 P... [more]
A0A1S3CAV11.1e-26484.60protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103498725 PE=3 ... [more]
A0A6J1DNR22.6e-25881.67protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC11102235... [more]
A0A5A7T8K12.9e-24680.22Proton-dependent oligopeptide transporter family OS=Cucumis melo var. makuwa OX=... [more]
Match NameE-valueIdentityDescription
AT1G22540.16.8e-18757.60Major facilitator superfamily protein [more]
AT1G72120.11.7e-17456.83Major facilitator superfamily protein [more]
AT1G72125.14.7e-17255.58Major facilitator superfamily protein [more]
AT1G22550.12.4e-16051.59Major facilitator superfamily protein [more]
AT1G72140.15.4e-16050.97Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 18..436
e-value: 8.2E-93
score: 311.3
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 2..473
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..475
e-value: 4.7E-160
score: 535.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 2..468
NoneNo IPR availablePANTHERPTHR11654:SF510FACILITATOR SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 2..473
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 7..31
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 1..472
score: 8.931659

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0020075.1Tan0020075.1mRNA
Tan0020075.2Tan0020075.2mRNA
Tan0020075.3Tan0020075.3mRNA
Tan0020075.4Tan0020075.4mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0042938 dipeptide transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity