Tan0019993 (gene) Snake gourd v1

Overview
NameTan0019993
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionCalcium-binding EF-hand family protein
LocationLG07: 66011142 .. 66011567 (+)
RNA-Seq ExpressionTan0019993
SyntenyTan0019993
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAATTATGGTGCTACAAGAGAGTGTTCGAGCATTTTGACGTGGATGGGAACGGGAAGATTTCCCCATCGGAGCTGCGGGAGTGCATAGCGACGGTGTCGGGGGAGGAGCTGTCGATGGTGGAGGCAGAGGAGGTGGTGTCAGCGTTCGACTCGGACGGAGACGGTCAACTCGAGGCGGAGGAATTCATGAGGTTCGTGGACGGCGGAAGGGAGGAGGAGAAGGTGAAGGAGCTTAGAGAAGCTTTTAAAATGTATGAAATGGAGGGATCTGGATTGATTACGGCGGTGAGCTTGAGGAGGATGCTGAGGAAATTGGGAGAGTCTAAAAGTTTGGAGGATTGTAAGGCCATGATTGCTAAGTTTGACCTTAATGGTGATGGTGTTCTTAGTTTTGAGGAGTTTAAGGTTATGATGTCATGA

mRNA sequence

ATGGAGAAATTATGGTGCTACAAGAGAGTGTTCGAGCATTTTGACGTGGATGGGAACGGGAAGATTTCCCCATCGGAGCTGCGGGAGTGCATAGCGACGGTGTCGGGGGAGGAGCTGTCGATGGTGGAGGCAGAGGAGGTGGTGTCAGCGTTCGACTCGGACGGAGACGGTCAACTCGAGGCGGAGGAATTCATGAGGTTCGTGGACGGCGGAAGGGAGGAGGAGAAGGTGAAGGAGCTTAGAGAAGCTTTTAAAATGTATGAAATGGAGGGATCTGGATTGATTACGGCGGTGAGCTTGAGGAGGATGCTGAGGAAATTGGGAGAGTCTAAAAGTTTGGAGGATTGTAAGGCCATGATTGCTAAGTTTGACCTTAATGGTGATGGTGTTCTTAGTTTTGAGGAGTTTAAGGTTATGATGTCATGA

Coding sequence (CDS)

ATGGAGAAATTATGGTGCTACAAGAGAGTGTTCGAGCATTTTGACGTGGATGGGAACGGGAAGATTTCCCCATCGGAGCTGCGGGAGTGCATAGCGACGGTGTCGGGGGAGGAGCTGTCGATGGTGGAGGCAGAGGAGGTGGTGTCAGCGTTCGACTCGGACGGAGACGGTCAACTCGAGGCGGAGGAATTCATGAGGTTCGTGGACGGCGGAAGGGAGGAGGAGAAGGTGAAGGAGCTTAGAGAAGCTTTTAAAATGTATGAAATGGAGGGATCTGGATTGATTACGGCGGTGAGCTTGAGGAGGATGCTGAGGAAATTGGGAGAGTCTAAAAGTTTGGAGGATTGTAAGGCCATGATTGCTAAGTTTGACCTTAATGGTGATGGTGTTCTTAGTTTTGAGGAGTTTAAGGTTATGATGTCATGA

Protein sequence

MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLEAEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMIAKFDLNGDGVLSFEEFKVMMS
Homology
BLAST of Tan0019993 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.2e-33
Identity = 74/137 (54.01%), Postives = 100/137 (72.99%), Query Frame = 0

Query: 7   YKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLEAEEFMR 66
           ++RVF  FD D +GKIS +ELR C+    GE++S  EAE +VS+ D+D DG L+ EEF +
Sbjct: 8   FRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEFTK 67

Query: 67  F---VDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMIAKF 126
               ++ G EEE+ + L EAF+MYEMEG G IT  SL+RML KLG  + +E+C+ MI +F
Sbjct: 68  LAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMICRF 127

Query: 127 DLNGDGVLSFEEFKVMM 141
           DL+GDGV+SFEEFK+MM
Sbjct: 128 DLDGDGVISFEEFKIMM 144

BLAST of Tan0019993 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.2e-33
Identity = 76/136 (55.88%), Postives = 99/136 (72.79%), Query Frame = 0

Query: 10  VFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLEAEEFMRFVD 69
           +F  FD DG+G+IS +ELR C+ T  GEE+S  EA ++V++ D+DGDG L   EF+R V 
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 70  GGREEEKVKE----LREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMIAKFDL 129
               EE+ +     LREAF MYEMEG G IT  SLRRMLR+LG  + ++DC+AMI +FDL
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 130 NGDGVLSFEEFKVMMS 142
           NGDGVLSF+EFK+MM+
Sbjct: 135 NGDGVLSFDEFKIMMN 150

BLAST of Tan0019993 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 2.2e-30
Identity = 73/143 (51.05%), Postives = 97/143 (67.83%), Query Frame = 0

Query: 7   YKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLEAEEFMR 66
           ++RVF  FD DG+GKIS +ELR C+    GE++   E + +++  D+DGDG L+ EEF+R
Sbjct: 7   FRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVR 66

Query: 67  FV-----DGGREEEKVKE----LREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCK 126
            V     DG  EE+   E    LREAF MYEMEG G IT +SL+ ML KLG    + +C+
Sbjct: 67  LVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAECQ 126

Query: 127 AMIAKFDLNGDGVLSFEEFKVMM 141
           AMI +FD+NGDGVL+F+EFK MM
Sbjct: 127 AMICRFDMNGDGVLTFDEFKTMM 149

BLAST of Tan0019993 vs. ExPASy Swiss-Prot
Match: Q2QYW1 (Probable calcium-binding protein CML25/26 OS=Oryza sativa subsp. japonica OX=39947 GN=CML25 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 7.1e-29
Identity = 74/139 (53.24%), Postives = 96/139 (69.06%), Query Frame = 0

Query: 10  VFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLEAEEFMRFVD 69
           VF  FD DG+GK+S SELR C+A   GEE+S  EA  +++  D+DGDG L+  EFMR   
Sbjct: 8   VFAAFDKDGDGKVSASELRGCMAAALGEEVSEEEAAAILATADTDGDGLLDHHEFMRLSA 67

Query: 70  GGREEEKVKE----LREAFKMY-EMEGSGLITAVSLRRMLRKLG---ESKSLEDCKAMIA 129
             + +E  +E    LREAF MY E E + +IT  SLRRMLR+LG   +   +E+C+AMI 
Sbjct: 68  AHQLQEPAEESLRCLREAFDMYAEEEETAVITPASLRRMLRRLGSEHQRLEMEECRAMIC 127

Query: 130 KFDLNGDGVLSFEEFKVMM 141
           +FDLNGDGVLSF+EF+VMM
Sbjct: 128 RFDLNGDGVLSFDEFRVMM 146

BLAST of Tan0019993 vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 9.2e-29
Identity = 70/135 (51.85%), Postives = 94/135 (69.63%), Query Frame = 0

Query: 8   KRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLEAEEFMRF 67
           + VF++ D + +GKIS  EL+ C++ + G  LS  E EEVV   D DGDG ++ EEF++ 
Sbjct: 51  RTVFDYMDANSDGKISGEELQSCVSLLGG-ALSSREVEEVVKTSDVDGDGFIDFEEFLKL 110

Query: 68  VDG--GREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMIAKFDL 127
           ++G  G +EE+ KEL+EAF MY MEG   ITA SLRR L +LGES +++ CK MI  FD 
Sbjct: 111 MEGEDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQ 170

Query: 128 NGDGVLSFEEFKVMM 141
           N DGVLSF+EF +MM
Sbjct: 171 NDDGVLSFDEFVLMM 184

BLAST of Tan0019993 vs. NCBI nr
Match: XP_008450085.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis melo] >KAA0042870.1 putative calcium-binding protein CML19 [Cucumis melo var. makuwa] >TYK05273.1 putative calcium-binding protein CML19 [Cucumis melo var. makuwa])

HSP 1 Score: 239.2 bits (609), Expect = 2.2e-59
Identity = 118/141 (83.69%), Postives = 132/141 (93.62%), Query Frame = 0

Query: 1   MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLE 60
           MEKLWCYKRVFEHFD DG+GKISPSELRECIA VSGE+LS+VEA+E V+ FDSDGDGQLE
Sbjct: 1   MEKLWCYKRVFEHFDSDGDGKISPSELRECIAVVSGEKLSVVEAQEAVAEFDSDGDGQLE 60

Query: 61  AEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMI 120
            E+FMRFVDGG +EEKVKELREAFKMYEMEGSGLITA SLRRMLRK+GE++SL DC AMI
Sbjct: 61  EEDFMRFVDGGGKEEKVKELREAFKMYEMEGSGLITAESLRRMLRKMGETRSLRDCMAMI 120

Query: 121 AKFDLNGDGVLSFEEFKVMMS 142
           AKFD++GDGVL+F+EFKVMMS
Sbjct: 121 AKFDVDGDGVLNFDEFKVMMS 141

BLAST of Tan0019993 vs. NCBI nr
Match: XP_038906141.1 (putative calcium-binding protein CML19 [Benincasa hispida])

HSP 1 Score: 236.1 bits (601), Expect = 1.8e-58
Identity = 120/142 (84.51%), Postives = 131/142 (92.25%), Query Frame = 0

Query: 1   MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLE 60
           MEKLWCYKRVFEHFD DG+GKISPSELRECIA VSGE+LSMVEAEE ++ FDSDGDGQLE
Sbjct: 1   MEKLWCYKRVFEHFDSDGDGKISPSELRECIAVVSGEKLSMVEAEEAMAEFDSDGDGQLE 60

Query: 61  AEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESK-SLEDCKAM 120
            E+F+RFVDGG E+EKVKELREAFKMYEMEGSG ITA SLRRM +KLGE+K SL DCKAM
Sbjct: 61  EEDFVRFVDGGGEKEKVKELREAFKMYEMEGSGFITAESLRRMFKKLGETKISLGDCKAM 120

Query: 121 IAKFDLNGDGVLSFEEFKVMMS 142
           IAKFDL+GDGVLSF+EFKVMMS
Sbjct: 121 IAKFDLDGDGVLSFDEFKVMMS 142

BLAST of Tan0019993 vs. NCBI nr
Match: XP_004145714.1 (putative calcium-binding protein CML19 [Cucumis sativus])

HSP 1 Score: 228.0 bits (580), Expect = 5.0e-56
Identity = 114/141 (80.85%), Postives = 130/141 (92.20%), Query Frame = 0

Query: 1   MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLE 60
           MEK  CYKRVFEHFD DG+GKISPSELRECIA VSGE+LS++EA+E V+ FDSDGDGQL+
Sbjct: 1   MEKPRCYKRVFEHFDSDGDGKISPSELRECIAVVSGEKLSVLEAQEAVAEFDSDGDGQLK 60

Query: 61  AEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMI 120
            E+F+RFVDGG EEE+VKELREAFKMYEM+GSGLITA SLRRMLRKLGE+KSL DC AMI
Sbjct: 61  EEDFVRFVDGGGEEERVKELREAFKMYEMKGSGLITAESLRRMLRKLGETKSLRDCTAMI 120

Query: 121 AKFDLNGDGVLSFEEFKVMMS 142
           AKFD++GDGVLSF+EF+VMMS
Sbjct: 121 AKFDIDGDGVLSFDEFRVMMS 141

BLAST of Tan0019993 vs. NCBI nr
Match: KAG7022675.1 (putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 224.6 bits (571), Expect = 5.5e-55
Identity = 114/140 (81.43%), Postives = 126/140 (90.00%), Query Frame = 0

Query: 1   MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLE 60
           M+KL CYKRVF+HFD+DG+GKISP E+RECIA +SG E+SMVEAEEVV+AFD DGDG L+
Sbjct: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60

Query: 61  AEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMI 120
            EEF RFVD G EEEKVKELREAFKMYEMEGSGLITAVSLRR L+KLGE  SLEDC AMI
Sbjct: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLNGDGVLSFEEFKVMM 141
           AKFDL+GDGVLSF+EFKVMM
Sbjct: 121 AKFDLDGDGVLSFDEFKVMM 140

BLAST of Tan0019993 vs. NCBI nr
Match: KAE8650780.1 (hypothetical protein Csa_017647 [Cucumis sativus])

HSP 1 Score: 221.1 bits (562), Expect = 6.1e-54
Identity = 110/137 (80.29%), Postives = 126/137 (91.97%), Query Frame = 0

Query: 1   MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLE 60
           MEK  CYKRVFEHFD DG+GKISPSELRECIA VSGE+LS++EA+E V+ FDSDGDGQL+
Sbjct: 1   MEKPRCYKRVFEHFDSDGDGKISPSELRECIAVVSGEKLSVLEAQEAVAEFDSDGDGQLK 60

Query: 61  AEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMI 120
            E+F+RFVDGG EEE+VKELREAFKMYEM+GSGLITA SLRRMLRKLGE+KSL DC AMI
Sbjct: 61  EEDFVRFVDGGGEEERVKELREAFKMYEMKGSGLITAESLRRMLRKLGETKSLRDCTAMI 120

Query: 121 AKFDLNGDGVLSFEEFK 138
           AKFD++GDGVLSF+EF+
Sbjct: 121 AKFDIDGDGVLSFDEFR 137

BLAST of Tan0019993 vs. ExPASy TrEMBL
Match: A0A5D3BZX1 (Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold108G00900 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.0e-59
Identity = 118/141 (83.69%), Postives = 132/141 (93.62%), Query Frame = 0

Query: 1   MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLE 60
           MEKLWCYKRVFEHFD DG+GKISPSELRECIA VSGE+LS+VEA+E V+ FDSDGDGQLE
Sbjct: 1   MEKLWCYKRVFEHFDSDGDGKISPSELRECIAVVSGEKLSVVEAQEAVAEFDSDGDGQLE 60

Query: 61  AEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMI 120
            E+FMRFVDGG +EEKVKELREAFKMYEMEGSGLITA SLRRMLRK+GE++SL DC AMI
Sbjct: 61  EEDFMRFVDGGGKEEKVKELREAFKMYEMEGSGLITAESLRRMLRKMGETRSLRDCMAMI 120

Query: 121 AKFDLNGDGVLSFEEFKVMMS 142
           AKFD++GDGVL+F+EFKVMMS
Sbjct: 121 AKFDVDGDGVLNFDEFKVMMS 141

BLAST of Tan0019993 vs. ExPASy TrEMBL
Match: A0A1S3BNF9 (putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103491776 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.0e-59
Identity = 118/141 (83.69%), Postives = 132/141 (93.62%), Query Frame = 0

Query: 1   MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLE 60
           MEKLWCYKRVFEHFD DG+GKISPSELRECIA VSGE+LS+VEA+E V+ FDSDGDGQLE
Sbjct: 1   MEKLWCYKRVFEHFDSDGDGKISPSELRECIAVVSGEKLSVVEAQEAVAEFDSDGDGQLE 60

Query: 61  AEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMI 120
            E+FMRFVDGG +EEKVKELREAFKMYEMEGSGLITA SLRRMLRK+GE++SL DC AMI
Sbjct: 61  EEDFMRFVDGGGKEEKVKELREAFKMYEMEGSGLITAESLRRMLRKMGETRSLRDCMAMI 120

Query: 121 AKFDLNGDGVLSFEEFKVMMS 142
           AKFD++GDGVL+F+EFKVMMS
Sbjct: 121 AKFDVDGDGVLNFDEFKVMMS 141

BLAST of Tan0019993 vs. ExPASy TrEMBL
Match: A0A0A0L8P1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G588520 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.4e-56
Identity = 114/141 (80.85%), Postives = 130/141 (92.20%), Query Frame = 0

Query: 1   MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLE 60
           MEK  CYKRVFEHFD DG+GKISPSELRECIA VSGE+LS++EA+E V+ FDSDGDGQL+
Sbjct: 1   MEKPRCYKRVFEHFDSDGDGKISPSELRECIAVVSGEKLSVLEAQEAVAEFDSDGDGQLK 60

Query: 61  AEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMI 120
            E+F+RFVDGG EEE+VKELREAFKMYEM+GSGLITA SLRRMLRKLGE+KSL DC AMI
Sbjct: 61  EEDFVRFVDGGGEEERVKELREAFKMYEMKGSGLITAESLRRMLRKLGETKSLRDCTAMI 120

Query: 121 AKFDLNGDGVLSFEEFKVMMS 142
           AKFD++GDGVLSF+EF+VMMS
Sbjct: 121 AKFDIDGDGVLSFDEFRVMMS 141

BLAST of Tan0019993 vs. ExPASy TrEMBL
Match: A0A6J1ENP8 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111435189 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 3.9e-54
Identity = 112/140 (80.00%), Postives = 124/140 (88.57%), Query Frame = 0

Query: 1   MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLE 60
           M+KL CYKRVF+HFD DG+GKISP E+RECIA +SG E+SMVEAEEVV+AFD DGDG L+
Sbjct: 1   MDKLSCYKRVFDHFDTDGDGKISPFEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60

Query: 61  AEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMI 120
            EEF RFVD G EEEKVKELREAFKMYEM+GSGLITAVSLRR L+KLGE  SLEDC AMI
Sbjct: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMDGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLNGDGVLSFEEFKVMM 141
           AKFDL+ DGVLSF+EFKVMM
Sbjct: 121 AKFDLDSDGVLSFDEFKVMM 140

BLAST of Tan0019993 vs. ExPASy TrEMBL
Match: A0A6J1JR73 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111486733 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.5e-53
Identity = 113/140 (80.71%), Postives = 124/140 (88.57%), Query Frame = 0

Query: 1   MEKLWCYKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLE 60
           M+KL CYKRVF+HFD+D +GKISP E+RECIA VSGEE+SMVEAEEVV AFD DGDG L+
Sbjct: 1   MDKLSCYKRVFDHFDMDRDGKISPFEVRECIAAVSGEEVSMVEAEEVVGAFDMDGDGGLD 60

Query: 61  AEEFMRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMI 120
            EEF RFVD   EEEKVKELR+AFKMYEMEGSGLITAVSLRR L+KLGE  SLEDC AMI
Sbjct: 61  VEEFERFVDDIGEEEKVKELRKAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLNGDGVLSFEEFKVMM 141
           AKFDL+GDGVLSF+EFKVMM
Sbjct: 121 AKFDLDGDGVLSFDEFKVMM 140

BLAST of Tan0019993 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 127.9 bits (320), Expect = 6.6e-30
Identity = 70/135 (51.85%), Postives = 94/135 (69.63%), Query Frame = 0

Query: 8   KRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLEAEEFMRF 67
           + VF++ D + +GKIS  EL+ C++ + G  LS  E EEVV   D DGDG ++ EEF++ 
Sbjct: 51  RTVFDYMDANSDGKISGEELQSCVSLLGG-ALSSREVEEVVKTSDVDGDGFIDFEEFLKL 110

Query: 68  VDG--GREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMIAKFDL 127
           ++G  G +EE+ KEL+EAF MY MEG   ITA SLRR L +LGES +++ CK MI  FD 
Sbjct: 111 MEGEDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQ 170

Query: 128 NGDGVLSFEEFKVMM 141
           N DGVLSF+EF +MM
Sbjct: 171 NDDGVLSFDEFVLMM 184

BLAST of Tan0019993 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 122.5 bits (306), Expect = 2.8e-28
Identity = 66/131 (50.38%), Postives = 90/131 (68.70%), Query Frame = 0

Query: 10  VFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLEAEEFMRFVD 69
           VF + D + +G+ISP EL++   T+ GE+LS  EA   V   D+DGDG L+ EEF + + 
Sbjct: 47  VFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLIK 106

Query: 70  GGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMIAKFDLNGDG 129
              EEEK  EL+ AF++Y  EG   IT  SL+ ML+KLGES++ +DC+ MI+ FDLN DG
Sbjct: 107 VDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNADG 166

Query: 130 VLSFEEFKVMM 141
           VLSF+EF +MM
Sbjct: 167 VLSFDEFALMM 176

BLAST of Tan0019993 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 122.5 bits (306), Expect = 2.8e-28
Identity = 66/131 (50.38%), Postives = 90/131 (68.70%), Query Frame = 0

Query: 10  VFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLEAEEFMRFVD 69
           VF + D + +G+ISP EL++   T+ GE+LS  EA   V   D+DGDG L+ EEF + + 
Sbjct: 47  VFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLIK 106

Query: 70  GGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMIAKFDLNGDG 129
              EEEK  EL+ AF++Y  EG   IT  SL+ ML+KLGES++ +DC+ MI+ FDLN DG
Sbjct: 107 VDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNADG 166

Query: 130 VLSFEEFKVMM 141
           VLSF+EF +MM
Sbjct: 167 VLSFDEFALMM 176

BLAST of Tan0019993 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 116.3 bits (290), Expect = 2.0e-26
Identity = 65/134 (48.51%), Postives = 87/134 (64.93%), Query Frame = 0

Query: 10  VFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVVSAFDSDGDGQLEAEEFMRFVD 69
           VF + D + +G+IS  EL++   T+ GE++S  EAE  V   D DGDG L+  EF   + 
Sbjct: 26  VFAYMDANRDGRISAEELKKSFKTL-GEQMSDEEAEAAVKLSDIDGDGMLDINEFALLIK 85

Query: 70  GG---REEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMIAKFDLN 129
           G     EEEK +++ EAF+MY  +G   IT  SL+ ML KLGES++ +DCK MI  FDLN
Sbjct: 86  GNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDLN 145

Query: 130 GDGVLSFEEFKVMM 141
            DGVLSF+EF +MM
Sbjct: 146 ADGVLSFDEFALMM 158

BLAST of Tan0019993 vs. TAIR 10
Match: AT3G01830.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 113.6 bits (283), Expect = 1.3e-25
Identity = 61/136 (44.85%), Postives = 92/136 (67.65%), Query Frame = 0

Query: 7   YKRVFEHFDVDGNGKISPSELRECIATVSGEELSMVEAEEVV--SAFDSDGDGQLEAEEF 66
           Y+RVF  FD    GK+S S +  C+  +   + ++V+ E+    +  +S  D  LE E+F
Sbjct: 12  YQRVFSCFDKSHQGKVSVSTIERCVDAIKSGKRAVVDQEDTTNPNPEESTDDKSLELEDF 71

Query: 67  MRFVDGGREEEKVKELREAFKMYEMEGSGLITAVSLRRMLRKLGESKSLEDCKAMIAKFD 126
           ++ V+ G E +K K+L+EAFK+YE E  G IT  SL+RML  LGESKSL+DC+ MI++FD
Sbjct: 72  VKLVEEGEEADKEKDLKEAFKLYE-ESEG-ITPKSLKRMLSLLGESKSLKDCEVMISQFD 131

Query: 127 LNGDGVLSFEEFKVMM 141
           +N DG+++F+EF+ MM
Sbjct: 132 INRDGIINFDEFRAMM 145

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RYJ81.2e-3354.01Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q8RYK01.2e-3355.88Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q8RYJ92.2e-3051.05Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q2QYW17.1e-2953.24Probable calcium-binding protein CML25/26 OS=Oryza sativa subsp. japonica OX=399... [more]
Q9FIH99.2e-2951.85Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_008450085.12.2e-5983.69PREDICTED: putative calcium-binding protein CML19 [Cucumis melo] >KAA0042870.1 p... [more]
XP_038906141.11.8e-5884.51putative calcium-binding protein CML19 [Benincasa hispida][more]
XP_004145714.15.0e-5680.85putative calcium-binding protein CML19 [Cucumis sativus][more]
KAG7022675.15.5e-5581.43putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. a... [more]
KAE8650780.16.1e-5480.29hypothetical protein Csa_017647 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A5D3BZX11.0e-5983.69Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BNF91.0e-5983.69putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103491776 P... [more]
A0A0A0L8P12.4e-5680.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G588520 PE=4 SV=1[more]
A0A6J1ENP83.9e-5480.00putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1JR732.5e-5380.71putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114867... [more]
Match NameE-valueIdentityDescription
AT5G42380.16.6e-3051.85calmodulin like 37 [more]
AT1G76650.12.8e-2850.38calmodulin-like 38 [more]
AT1G76650.32.8e-2850.38calmodulin-like 38 [more]
AT1G76640.12.0e-2648.51Calcium-binding EF-hand family protein [more]
AT3G01830.11.3e-2544.85Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 115..141
e-value: 0.043
score: 23.0
coord: 79..107
e-value: 0.83
score: 18.4
coord: 43..71
e-value: 0.8
score: 18.5
coord: 6..34
e-value: 1.8E-4
score: 30.8
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 8..68
e-value: 3.4E-12
score: 46.6
coord: 79..140
e-value: 2.1E-11
score: 44.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 111..141
score: 15.350267
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 7..37
score: 12.337564
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 75..110
score: 10.608049
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 39..74
score: 10.914899
IPR002048EF-hand domainCDDcd00051EFhcoord: 79..141
e-value: 4.03858E-16
score: 66.0321
NoneNo IPR availableGENE3D1.10.238.10coord: 6..68
e-value: 3.4E-17
score: 64.3
NoneNo IPR availableGENE3D1.10.238.10coord: 69..141
e-value: 5.4E-22
score: 79.8
NoneNo IPR availablePANTHERPTHR10891:SF858EF HAND CALCIUM-BINDING FAMILY PROTEINcoord: 3..141
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 3..141
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 124..136
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 52..64
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 15..27
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 5..141

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0019993.1Tan0019993.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding