Homology
BLAST of Tan0019462 vs. ExPASy Swiss-Prot
Match:
Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)
HSP 1 Score: 950.3 bits (2455), Expect = 1.6e-275
Identity = 479/877 (54.62%), Postives = 626/877 (71.38%), Query Frame = 0
Query: 14 CVCNCGITESFKLAVSDHHT-YGHKIKYQSGKMDEARMVARGESKVGFLKSSHRNLSKAY 73
C N GIT ++K + T +KIK S + R G+ L+S SK
Sbjct: 13 CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQS--YQFSKIC 72
Query: 74 ATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRSGFTVYVEMSSLQ 133
A S S+E ++ + KV +Y FRT+ G LV V VEK R +++ V +SSL+
Sbjct: 73 A-SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLE 132
Query: 134 LTSVD-GKPMLSWGVYRSDSAIVTP-NFQSSLPDGTIGTTATPFVNTSEGNFAVELEFDA 193
L+ D + ++ WGVYRSDS+ P +F++S D TT T FV +S + LEFD
Sbjct: 133 LSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDG 192
Query: 194 KHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSIS-GDGSINFS 253
K PFYLSF LK E+ +HR T FC+PVGF G+P PLGLS D S NFS
Sbjct: 193 KESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFS 252
Query: 254 IFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVSYGYRCKGSK 313
FS ++ +VVLCLY+DS ++KP LELDLDPY+NR+G++WHAS ++ +FV YGYRCK +
Sbjct: 253 FFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETA 312
Query: 314 FHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFDWDREVRPNL 373
+VE I++DPYA ++ S+ + ++LG + K P+FDW +V PN+
Sbjct: 313 HSKEDVDVEGEPIVLDPYATVVGKSV----------SQKYLGSLSKSPSFDWGEDVSPNI 372
Query: 374 PMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLIEPIFPFDEK 433
P+EKL VYRLNVK FT +SS+LP+++AGTFSG+ +K+ H TLG NAVL+EPIF F E+
Sbjct: 373 PLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQ 432
Query: 434 EGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTHTAVDGALQG 493
+GPYFP HFFSP +IYGPS SA+NSMK MVK+LH+ G+EV+LEVVFTHTA GAL+G
Sbjct: 433 KGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRG 492
Query: 494 IDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGH 553
IDDSSYY+ R +L+ KS LNCNYP+VQQL+L+SLRYWVTEFH+DGFCFINASSLLRG
Sbjct: 493 IDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGV 552
Query: 554 HGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNSKFCNDIR 613
HGE LSRPPLVEAIAFDPLL++TK+IAD WDP E + E RFPHWKRWAE+N+++C ++R
Sbjct: 553 HGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVR 612
Query: 614 DFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELS 673
+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN+GL LVD+VSFS +LASELS
Sbjct: 613 NFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELS 672
Query: 674 WNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGSVAFNDRRSF 733
WNCGEEG T+ + VL++RLKQIRNF+F+ ++SLGVPVL+MGDECG S+ GS R+ F
Sbjct: 673 WNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPF 732
Query: 734 DWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSPPQWEDPSCK 793
DW+LL + FGTQ TQFI+F++S RA+R D++Q R+FLK ENI WY N+Q+ P+WEDP+ K
Sbjct: 733 DWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASK 792
Query: 794 FLAVILR--ADKEENGSITEDDKPRS-DIFMAFNASDQSESVALPEPSEGTSWFRLIDTA 853
FLA+ ++ +++EE S+ E ++P+S D+F+ FNASD ESV LP +G+ W RL+DTA
Sbjct: 793 FLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTA 852
Query: 854 LSFPGFFSSDGEPV----PATGSATYEMQAHSCALFE 880
L FPGFFS +GE V P YEM+ +SC LFE
Sbjct: 853 LPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of Tan0019462 vs. ExPASy Swiss-Prot
Match:
Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)
HSP 1 Score: 764.2 bits (1972), Expect = 1.6e-219
Identity = 375/718 (52.23%), Postives = 502/718 (69.92%), Query Frame = 0
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
+ A EL FD PFYLSFLL + +EIR+HRKTSF VPVG G G P+PLG+SI
Sbjct: 103 SLAAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVGVGPGSPAPLGMSI 162
Query: 241 SGDGSINFSIFSSTAESVVLCLY----NDSKSEKPVLELDLDPYINRSGNIWHASFESAL 300
SGDG++NF+++S A +V L LY ++P LE+DLDPYI+R+GN+WH S S
Sbjct: 163 SGDGAVNFAVYSKNANAVSLYLYAAAVGGGGGDEPALEIDLDPYIHRTGNVWHVSLASVD 222
Query: 301 NFVSYGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTS---RFLGQV 360
+VSY + C G + R L+DPYAK++ + G T+ R +
Sbjct: 223 GYVSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASL 282
Query: 361 CKVPAFDWDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITL 420
P+++W R+ P LP+EKL VYR NV FT D+SS LP D AGTF+GL+ K+ HF +L
Sbjct: 283 AIAPSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSL 342
Query: 421 GVNAVLIEPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVM 480
GVNA+L+EP+FPF + +GPYFP HFFSP N+Y S+SAI SMK+MV+ +H NG+EV+
Sbjct: 343 GVNAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVL 402
Query: 481 LEVVFTHTAVDG------ALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRY 540
LEVVFTHTA +++GID+SSYY N + + S LNCN+P+ Q+L+LDSLR+
Sbjct: 403 LEVVFTHTAEGESECQTISMRGIDNSSYYIANGIAGCKA-SILNCNHPVTQKLILDSLRH 462
Query: 541 WVTEFHIDGFCFINASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILN 600
WV +FH+DGFCFINA L+RG GE LSRPPL+EAI FDP+LS TKIIAD W P +
Sbjct: 463 WVLDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNV 522
Query: 601 ETRFPHWKRWAEVNSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIAR 660
+ FPHWKRWAEVN++F D+R F + E L+S LATRLCGSGD+FS RGPAFSFN ++R
Sbjct: 523 QFPFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSR 582
Query: 661 NAGLPLVDLVSFSSSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVL 720
N+GL LVDLVSFS+ L SE SWNCGEEGP+ N+ VL+ RL+QIRNF+F+LFVSLGVPVL
Sbjct: 583 NSGLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVL 642
Query: 721 HMGDECGQSSGGSVAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLK 780
+MGDECG S+ GSV++ DR +W +KT F + T FI+FL++ R++R D++Q R FLK
Sbjct: 643 NMGDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLK 702
Query: 781 GENIDWYGNNQSPPQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSES 840
ENI WYG++ P W+DP+ FL + + A+ +E + D R D+++ FNA+++S S
Sbjct: 703 LENIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANEESVS 762
Query: 841 VALPEPSEGTSWFRLIDTALSFPGFFSSDGEP--VPATGSATYEMQAHSCALFEAKSS 884
ALP +EG+ W RL+DT+L+FPGFF+++ P G ++Y ++AH+C LFE+KS+
Sbjct: 763 AALPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESKSA 798
BLAST of Tan0019462 vs. ExPASy Swiss-Prot
Match:
Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)
HSP 1 Score: 383.3 bits (983), Expect = 7.7e-105
Identity = 251/705 (35.60%), Postives = 360/705 (51.06%), Query Frame = 0
Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSKSEKPVLELDLDPYINRSG 290
G SPLG+S D INF++FS A SV LCL D + ++EL LDP +N++G
Sbjct: 88 GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147
Query: 291 NIWHASFES-ALNFVSYGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLG 350
+ WH E LN V YGYR G + S +L+DPYAK++ + G
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207
Query: 351 STSRFLGQV-CKVPAFDWDREVR-PNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSG 410
++F G + FDW + + PN+P + L +Y +NV+ FT D+SS + I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267
Query: 411 LTKKLLHFITLGVNAVLIEPIFPFDEKE--------------GPYFPSHFFSPTNIYGPS 470
+K+ H LG+NAV + P+F FDE E Y +FF+P + Y
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327
Query: 471 LGS-ISAINSMKEMVKELHANGVEVMLEVVFTHT-------AVDGALQGIDDSSYYFV-- 530
G I A KEMVK LH+ G+EV+L+VV+ HT + +GID+ YY +
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387
Query: 531 -NRVVNLEG-KSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGHHGELLSR 590
N+++N G + LNCN+P+V +L+LDSLR+WVTE+H+DGF F AS L R G LS
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447
Query: 591 PPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNSKFCNDIRDFFRGE- 650
PPL+ AIA D +LS+ KIIA+ WD L +FP+W RWAE N + +D+R F +G+
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGLYL-VGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507
Query: 651 GLVSSLATRLCGSGDVFS-DGRGPAFSFNFIARNAGLPLVDLVSFS-----------SSK 710
G+ S ATR+ GS D++ + R P NF+ + G L DLVS++ +
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567
Query: 711 LASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECG-------QS 770
SWNCG EG T +A + R +Q++NF L +S G P++ MGDE G S
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627
Query: 771 SGGSVAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGN 830
G + N+ + + D K ++ I F S + + NFL I W+ +
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHE-----NFLTQGEITWHED 687
Query: 831 NQSPPQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEG 882
N W++ KFLA L D DI++AFNA D +P+P G
Sbjct: 688 N-----WDNSESKFLAFTL-----------HDGIGGRDIYVAFNAHDYFVKALIPQPPPG 747
BLAST of Tan0019462 vs. ExPASy Swiss-Prot
Match:
B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)
HSP 1 Score: 377.1 bits (967), Expect = 5.5e-103
Identity = 255/730 (34.93%), Postives = 373/730 (51.10%), Query Frame = 0
Query: 208 GSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSISGDGSINFSIFSSTAESVVLCLY---- 267
G +E R + +P + G PLG+S +G +NF++FS A SV+LCL
Sbjct: 80 GRSMTEERGCTMSDTEMPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGR 139
Query: 268 -NDSKSEKPVLELDLDPYINRSGNIWHASFES-ALNFVSYGYRCKGSKFHANQDEVEVSR 327
+ + V+E LD N++G+IWH E + V YGYR G + + S
Sbjct: 140 GTEDEKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSST 199
Query: 328 ILVDPYAKILTASIPKTCGQGLGSTSRFLGQV-CKVPAFDWDREVR-PNLPMEKLFVYRL 387
+L+DPYAK+++ K G +S+ G FDW + R PNLP L +Y +
Sbjct: 200 VLLDPYAKLVSGR--KYFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEM 259
Query: 388 NVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLIEPIFPFDEKEGPYFPS--- 447
NV+ FT D+SS L + G++ GL K+ H + LGVNAV + P+F +DE E +P+
Sbjct: 260 NVRAFTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRD 319
Query: 448 -----------HFFSPTNIYGPSLGS-ISAINSMKEMVKELHANGVEVMLEVVFTHT--A 507
+FF+P + Y + G ++A +K+MVKELH G+EV+L+VV+ HT A
Sbjct: 320 HMVNTWGYSTINFFAPMSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEA 379
Query: 508 VDG-----ALQGIDDSSYYFVN-----RVVNLEG-KSALNCNYPIVQQLLLDSLRYWVTE 567
D + +GID+ YY ++ ++N G + LNCN+P+V++L+LDSLR+WV E
Sbjct: 380 DDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEE 439
Query: 568 FHIDGFCFINASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRF 627
+HIDGF F AS L RG G L PPL++ IA D +LS+ KIIA+ WD L RF
Sbjct: 440 YHIDGFRFDLASVLCRGPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLYL-VGRF 499
Query: 628 PHWKRWAEVNSKFCNDIRDFFRGE-GLVSSLATRLCGSGDVFS-DGRGPAFSFNFIARNA 687
P+W RWAE N K+ +D+R F +G+ G+ ATR+ GS D++ + R P NF+ +
Sbjct: 500 PNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHD 559
Query: 688 GLPLVDLVSFS-----------SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVL 747
G L DLVS++ SWNCG EG T++ VL R +Q++NF L
Sbjct: 560 GFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVAL 619
Query: 748 FVSLGVPVLHMGDECG-------QSSGGSVAFNDRRSFDWDLLKTGFGTQTTQFITFLSS 807
+S G P++ MGDE G S G N +F W+ L+ +F + +
Sbjct: 620 MISQGTPMMLMGDEYGHTRYGNNNSYGHDTCIN---NFQWEQLEQRRDGH-FRFFSEMIK 679
Query: 808 FRAKRFDLYQSRNFLKGENIDWYGNNQSPPQWEDPSCKFLAVILRADKEENGSITEDDKP 867
FR L + R FL ++ W+ + WE+ KFLA + D
Sbjct: 680 FRHSNPILRRDR-FLNKNDVTWHED-----CWENQESKFLAFTV-----------HDHNS 739
Query: 868 RSDIFMAFNASDQSESVALPEPSEGTSWFRLIDTALSFPGFFSSDGEPVPATGSATYEMQ 882
DI++AFNA D +P P W R++DT L P +G VP TG Y +
Sbjct: 740 GGDIYLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEG--VPFTG-PKYRIA 781
BLAST of Tan0019462 vs. ExPASy Swiss-Prot
Match:
D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)
HSP 1 Score: 350.9 bits (899), Expect = 4.2e-95
Identity = 231/694 (33.29%), Postives = 348/694 (50.14%), Query Frame = 0
Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY--NDSKSEKPVLELDLDPYINRSGNIW 290
G P+PLG + + DG +NF+++S+ A + LCL+ +D ++++ E+ LDP NR+GN+W
Sbjct: 99 GMPAPLG-ATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158
Query: 291 HASFESALNFVSYGYRCKGSKFHANQDEVEVSRILVDPYAK-ILTASIPKTCGQGLGSTS 350
H E L+ + YGYR G +VS ++VDPYAK +++ G G
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218
Query: 351 RFLGQV-CKVPAFDWDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKK 410
+ G + FDW ++ P + L +Y ++++ FT SS + + GT+ G K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNV--EHPGTYIGAISK 278
Query: 411 LLHFITLGVNAVLIEPIFPFDEKE----------GPYFPSHFFSPTNIYGPSLGSI---- 470
L + LGVN V + P F+E E Y +FFSP Y S G I
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRY--SSGGIRNCG 338
Query: 471 -SAINSMKEMVKELHANGVEVMLEVVFTHTAVDG------ALQGIDDSSYYFV---NRVV 530
AIN K V+E H G+EV+++VVF HTA + +GID+S+YY +
Sbjct: 339 RDAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFY 398
Query: 531 NLEG-KSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRG------------- 590
N G + NCN+P+V++ ++D LRYWVTE H+DGF F AS + RG
Sbjct: 399 NYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSP 458
Query: 591 ------HHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNS 650
G L+ PPL++ I+ DP+L K+IA+ WD + +FPHWK W+E N
Sbjct: 459 VEGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWD-AGGLYQVGQFPHWKIWSEWNG 518
Query: 651 KFCNDIRDFFRG-EGLVSSLATRLCGSGDVF-SDGRGPAFSFNFIARNAGLPLVDLVSFS 710
K+ + +R F +G +G A LCGS ++ + GR P S NF+ + G L DLV+++
Sbjct: 519 KYRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYN 578
Query: 711 SSKLAS-----------ELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHM 770
+S LSWNCGEEG + V R +Q+RNF L VS GVP+ +M
Sbjct: 579 KKYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYM 638
Query: 771 GDECGQSSGG--SVAFNDR--RSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNF 830
GDE G + GG + +D F WD K + +F + ++ FR K+ + +F
Sbjct: 639 GDEYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADF 698
Query: 831 LKGENIDWYGNNQSPPQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQS 860
+ + W+G+ P W + S +F+A + D+ + +I++AFNAS
Sbjct: 699 PTAQRLHWHGHQPGKPDWSETS-RFVA------------FSTKDETKGEIYVAFNASHLP 758
BLAST of Tan0019462 vs. NCBI nr
Match:
XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])
HSP 1 Score: 1564.7 bits (4050), Expect = 0.0e+00
Identity = 770/885 (87.01%), Postives = 808/885 (91.30%), Query Frame = 0
Query: 1 MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
MAT LSSLVIQPFCV N G+TESFKLAV DHHTYG KIKY SGKMDEARM+A GES+VG
Sbjct: 1 MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60
Query: 61 LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
KSSHRNLSK YATSGI V KSGQRFG GKSKE KVATYLFRTEFGDLVNVFV KT S
Sbjct: 61 TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
GF V VE+SS+QL S++ +LSWGVYRSDSAIVTPNFQSSLPD TIG T TPFV TS G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
FAVELEFDAKH PFYLSFLLKYPV V SG+SEIRSHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
SGDGSINFSIFSSTAESVVLCLYNDS SEKPVLELDLD YINRSG+IWHASFESA NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300
Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
YGYRCKGSKFH NQD VEV+RI+VDPYAKILTAS+ KT GQGLG +FLGQ+CKVPAFD
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLG-LPKFLGQICKVPAFD 360
Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
WD EV PNLPMEKLF YRLNVK FTVDKSSQLP DIAGTFSGLTKKLLHF LGVNAVL+
Sbjct: 361 WDGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
EPIF FDEKEG YFP HFFSP N YGPS SISAINSMK MVK+LHANGVEV+LEVVFTH
Sbjct: 421 EPIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTH 480
Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
TA+DGALQGIDDSSYYFVNRV NLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
NASSLLRGHHGE LSR P VEAIAFDPLLSKTK++ADFWDPQE + ET FPHWKRWAEV
Sbjct: 541 NASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEV 600
Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFCND+RDFFRGEGL+SSLATRLCGSGDVFS+GRGPAFSFNFI RN GLPLVDLVSFS
Sbjct: 601 NSKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
+SKLASELSWNCGEEGPTSN EVLEKRLKQIRNFIFV+FVSLGVPVL+MGDECGQS+GGS
Sbjct: 661 NSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVVFVSLGVPVLNMGDECGQSAGGS 720
Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
VAFNDRRSF+WDLLKT FGTQTTQFITFLSSFR++RFDL+QSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWEDPSCKFLAV+LRADKEENGSITE+ KPRSDIFMAFNASDQSE+VALPEPSEGTSWF
Sbjct: 781 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSETVALPEPSEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
RL+DTALSFPGFFSSDGE VP TGS TYEMQAHSCALFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEQVPTTGSVTYEMQAHSCALFEAKSFND 884
BLAST of Tan0019462 vs. NCBI nr
Match:
XP_022150231.1 (isoamylase 2, chloroplastic [Momordica charantia])
HSP 1 Score: 1532.3 bits (3966), Expect = 0.0e+00
Identity = 749/885 (84.63%), Postives = 799/885 (90.28%), Query Frame = 0
Query: 1 MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
MAT SSLV+QP+ V N G+TE+F+LA SDHHT+GH IK+QSGK DE RM+A GES+ GF
Sbjct: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
Query: 61 LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
KSS NLSKAYA+ GISVEK+G RFGISGKSKE+ KVATYLFRTE GDLVNV V KT S
Sbjct: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
GF V +E+SS+QL +++ PMLSWGVYRSDSA+VTPN QSSLPD TIGT TPFVNTSEG
Sbjct: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
FAVELEFDAKH+PFYLSFLLK+PV V SG+SEIRSHRKTSFCVPVGFGRGYPSPLG+SI
Sbjct: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
SGDGS+NFSIFSS+AESVVLCLYNDS SEKPV+ELDLD YINRSGNIWH SFES NFVS
Sbjct: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
YGY CKGSK H QD VE+SRILVDPYAK+LT S KT G GLG S+ LGQ+CKVPAFD
Sbjct: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
WD EVRPNL MEKLF YRLNVK FTVDKSSQL TDIAGTF+GLTKKLLHF LGVNAVL+
Sbjct: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
EPIFPFDEKEGPYFPSHFFSPTNIYGPS GSISAINSMKEMVKELHANG+EVMLEVVFT+
Sbjct: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
TA DG LQGIDDSSYYF NRV NLEGK+ALNCNYP+VQQLLLDSLRYWVTEFHIDGFCFI
Sbjct: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
NASSLLRG+ GELLSRPPLVEAIAFDPLLSKTKIIADFWDP ESI ETRFPHWKRWAEV
Sbjct: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
N KFC+D+RDFFRGEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
SSKLASELSWNCGEEGPTSN EVLEKRLK IRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
+AF DRR FDWDLLKTGFGTQTTQFITF SSFRA+RFDL+QSRNFLKGENIDWYGNN SP
Sbjct: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWEDPSCKFLAV+LRADKEENG E+ +P+SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
RLIDTALSFPGFFSSDGE VP S TYEMQAHSCALFEAKS +D
Sbjct: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 891
BLAST of Tan0019462 vs. NCBI nr
Match:
XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])
HSP 1 Score: 1527.7 bits (3954), Expect = 0.0e+00
Identity = 748/884 (84.62%), Postives = 798/884 (90.27%), Query Frame = 0
Query: 1 MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
MAT LSSLVIQPFCV NCG+TES KLAVSDH TYG K KYQSGKMDEARM+A GESKVG
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 61 LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
+KSSHRNLSKAY TSGI V KSGQR GI GKSKE+ KVATYLFRTEFGDLVNVFV KT S
Sbjct: 61 VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
FTV +E+SS+QL S D +LSW VYRSDSA VT NFQSS PDGT G T TPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
F+VELEFDAKH PFYLSF+LK+P+ V SG+SEIRSHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
SGDGSINFSIFSSTAESVVLCLYNDS SEKP+LELDLDPYINRSGNIWHASFE A NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
YGY+CKG K H NQD +EVSRI+VDPYAKIL SIPK+ QGLG +FLGQ+ VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
WD EVRPNLPMEKLFVYRLNVK FT+DKSSQLP DIAGTFSGLTKKLLH LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
EPIF FDEKEGPYFP HFFSPTN YGPS SISAINSMKEMVKELHANGVEV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
T+VDGALQGIDDSSYYF NRV NLE +SALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE + ETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFC+DIRDFFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
+S LASELSWNCGEEGPT+ +VLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
VAFNDRRSF+WDLLKT FGTQTTQFI FLSSFR++RFDL+QSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWED SCKFLAV+LRADKEEN SITE+ K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSN 885
R++DTAL FPGFFSSDGE VP TGS TYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884
BLAST of Tan0019462 vs. NCBI nr
Match:
XP_022953509.1 (isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata] >XP_022953510.1 isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata])
HSP 1 Score: 1526.1 bits (3950), Expect = 0.0e+00
Identity = 751/885 (84.86%), Postives = 800/885 (90.40%), Query Frame = 0
Query: 1 MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
MAT LSSL IQPFC N G TESFKLAVSD+HTYG K+ YQSGK+DEARM A GESKVGF
Sbjct: 1 MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60
Query: 61 LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
KS HRN+ +AYA S ISV KSGQ+FG GK++E KVATYLFRTEFGDLV FV K S
Sbjct: 61 TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120
Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
GF +E+SSL+L S+D +LSWGVYRS+SAIVTPNFQS LP+ TIG T TPFV TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
FAVELEFDAKH PFYLSFLLKYPV V SG+SEI+SHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
SGDGSINFSIFSSTAESVVLCLYNDS SEKPVLELDLDPYINRSGNIWHASFE A NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
YGYRC+GSKFH NQDE EVSRILVDPYAKILTASIPKT GQGLG S+FLGQ+C VPAF
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360
Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
WD EVRPNLPMEKLF+YRLNVKHFTVDKSSQLP D AGTFSGLT+KLLHF LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
EPIFPFDEK+GPYFP HFFSPTNIY PS SIS++NS+KEMVKELHANGVEV+LEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480
Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
TA+DGALQGIDDS YYFVNRV N EGKSALNCN+PIVQQLLLDSLR+WVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
NASSLL+GHHGELLSRPP VEAIAFDPLLSKTKIIADFWDPQES+ ETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600
Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKF NDI++FFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
SSKLASELSWNCGEEGPTSN EVLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
VAF DR+SF+WD LKT FGTQTTQFITFLSSFR++RFDL+QSRNFLK ENIDW+ NNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
P WEDPSCKFLAV+LRADKEE+GSITE+ KP+SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
RL+DTALSFPGFFSSDGEPVP T TY MQAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884
BLAST of Tan0019462 vs. NCBI nr
Match:
KAA0057598.1 (isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo var. makuwa])
HSP 1 Score: 1526.1 bits (3950), Expect = 0.0e+00
Identity = 747/884 (84.50%), Postives = 797/884 (90.16%), Query Frame = 0
Query: 1 MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
MAT LSSLVIQPFCV NCG+TES KLAVSDH TYG K KYQSGKMDEARM+A GESKVG
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 61 LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
+KSSHRNLSKAY TSGISV KSGQR GI GKSKE+ KVATYLFRTEFGDLVNVFV KT S
Sbjct: 61 VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
FTV +E+SS+QL S D +LSW VYRSDSA VT NFQSS PDGT G T TPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
F+VELEFDAKH PFYLSF+LK+P+ V SG+SEIRSHRKTSF VPVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
SGDGSINFSIFSSTAESVVLCLYNDS SEKP+LELDLDPYINRSGNIWHASFE NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300
Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
YGY+CKG K H NQD +EVSRI+VDPYAKIL SIPK+ QGLG +FLGQ+ VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
WD EVRPNLPMEKLFVYRLNVK FT+DKSSQLP DIAGTFSGLTKKLLH LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
EPIF FDEKEGPYFP HFFSPTN YGPS SISAINSMKEMVKELHANGVEV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
T+VDGALQGIDDSSYYF NRV NLE +SALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE + ETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFC+DIRDFFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
+S LASELSWNCGEEGPT+ +VLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
VAFNDRRSF+WDLLKT FGTQTTQFI FLSSFR++RFDL+QSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWED SCKFLAV+LRADKEEN SITE+ K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSN 885
R++DTAL FPGFFSSDGE VP TGS TYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884
BLAST of Tan0019462 vs. ExPASy TrEMBL
Match:
A0A6J1DAX4 (isoamylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018448 PE=4 SV=1)
HSP 1 Score: 1532.3 bits (3966), Expect = 0.0e+00
Identity = 749/885 (84.63%), Postives = 799/885 (90.28%), Query Frame = 0
Query: 1 MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
MAT SSLV+QP+ V N G+TE+F+LA SDHHT+GH IK+QSGK DE RM+A GES+ GF
Sbjct: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
Query: 61 LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
KSS NLSKAYA+ GISVEK+G RFGISGKSKE+ KVATYLFRTE GDLVNV V KT S
Sbjct: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
GF V +E+SS+QL +++ PMLSWGVYRSDSA+VTPN QSSLPD TIGT TPFVNTSEG
Sbjct: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
FAVELEFDAKH+PFYLSFLLK+PV V SG+SEIRSHRKTSFCVPVGFGRGYPSPLG+SI
Sbjct: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
SGDGS+NFSIFSS+AESVVLCLYNDS SEKPV+ELDLD YINRSGNIWH SFES NFVS
Sbjct: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
YGY CKGSK H QD VE+SRILVDPYAK+LT S KT G GLG S+ LGQ+CKVPAFD
Sbjct: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
WD EVRPNL MEKLF YRLNVK FTVDKSSQL TDIAGTF+GLTKKLLHF LGVNAVL+
Sbjct: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
EPIFPFDEKEGPYFPSHFFSPTNIYGPS GSISAINSMKEMVKELHANG+EVMLEVVFT+
Sbjct: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
TA DG LQGIDDSSYYF NRV NLEGK+ALNCNYP+VQQLLLDSLRYWVTEFHIDGFCFI
Sbjct: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
NASSLLRG+ GELLSRPPLVEAIAFDPLLSKTKIIADFWDP ESI ETRFPHWKRWAEV
Sbjct: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
N KFC+D+RDFFRGEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
SSKLASELSWNCGEEGPTSN EVLEKRLK IRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
+AF DRR FDWDLLKTGFGTQTTQFITF SSFRA+RFDL+QSRNFLKGENIDWYGNN SP
Sbjct: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWEDPSCKFLAV+LRADKEENG E+ +P+SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
RLIDTALSFPGFFSSDGE VP S TYEMQAHSCALFEAKS +D
Sbjct: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 891
BLAST of Tan0019462 vs. ExPASy TrEMBL
Match:
A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)
HSP 1 Score: 1527.7 bits (3954), Expect = 0.0e+00
Identity = 748/884 (84.62%), Postives = 798/884 (90.27%), Query Frame = 0
Query: 1 MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
MAT LSSLVIQPFCV NCG+TES KLAVSDH TYG K KYQSGKMDEARM+A GESKVG
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 61 LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
+KSSHRNLSKAY TSGI V KSGQR GI GKSKE+ KVATYLFRTEFGDLVNVFV KT S
Sbjct: 61 VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
FTV +E+SS+QL S D +LSW VYRSDSA VT NFQSS PDGT G T TPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
F+VELEFDAKH PFYLSF+LK+P+ V SG+SEIRSHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
SGDGSINFSIFSSTAESVVLCLYNDS SEKP+LELDLDPYINRSGNIWHASFE A NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
YGY+CKG K H NQD +EVSRI+VDPYAKIL SIPK+ QGLG +FLGQ+ VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
WD EVRPNLPMEKLFVYRLNVK FT+DKSSQLP DIAGTFSGLTKKLLH LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
EPIF FDEKEGPYFP HFFSPTN YGPS SISAINSMKEMVKELHANGVEV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
T+VDGALQGIDDSSYYF NRV NLE +SALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE + ETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFC+DIRDFFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
+S LASELSWNCGEEGPT+ +VLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
VAFNDRRSF+WDLLKT FGTQTTQFI FLSSFR++RFDL+QSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWED SCKFLAV+LRADKEEN SITE+ K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSN 885
R++DTAL FPGFFSSDGE VP TGS TYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884
BLAST of Tan0019462 vs. ExPASy TrEMBL
Match:
A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)
HSP 1 Score: 1526.1 bits (3950), Expect = 0.0e+00
Identity = 751/885 (84.86%), Postives = 800/885 (90.40%), Query Frame = 0
Query: 1 MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
MAT LSSL IQPFC N G TESFKLAVSD+HTYG K+ YQSGK+DEARM A GESKVGF
Sbjct: 1 MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60
Query: 61 LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
KS HRN+ +AYA S ISV KSGQ+FG GK++E KVATYLFRTEFGDLV FV K S
Sbjct: 61 TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120
Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
GF +E+SSL+L S+D +LSWGVYRS+SAIVTPNFQS LP+ TIG T TPFV TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
FAVELEFDAKH PFYLSFLLKYPV V SG+SEI+SHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
SGDGSINFSIFSSTAESVVLCLYNDS SEKPVLELDLDPYINRSGNIWHASFE A NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
YGYRC+GSKFH NQDE EVSRILVDPYAKILTASIPKT GQGLG S+FLGQ+C VPAF
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360
Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
WD EVRPNLPMEKLF+YRLNVKHFTVDKSSQLP D AGTFSGLT+KLLHF LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
EPIFPFDEK+GPYFP HFFSPTNIY PS SIS++NS+KEMVKELHANGVEV+LEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480
Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
TA+DGALQGIDDS YYFVNRV N EGKSALNCN+PIVQQLLLDSLR+WVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
NASSLL+GHHGELLSRPP VEAIAFDPLLSKTKIIADFWDPQES+ ETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600
Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKF NDI++FFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
SSKLASELSWNCGEEGPTSN EVLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
VAF DR+SF+WD LKT FGTQTTQFITFLSSFR++RFDL+QSRNFLK ENIDW+ NNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
P WEDPSCKFLAV+LRADKEE+GSITE+ KP+SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
RL+DTALSFPGFFSSDGEPVP T TY MQAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884
BLAST of Tan0019462 vs. ExPASy TrEMBL
Match:
A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)
HSP 1 Score: 1526.1 bits (3950), Expect = 0.0e+00
Identity = 747/884 (84.50%), Postives = 797/884 (90.16%), Query Frame = 0
Query: 1 MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
MAT LSSLVIQPFCV NCG+TES KLAVSDH TYG K KYQSGKMDEARM+A GESKVG
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 61 LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
+KSSHRNLSKAY TSGISV KSGQR GI GKSKE+ KVATYLFRTEFGDLVNVFV KT S
Sbjct: 61 VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
FTV +E+SS+QL S D +LSW VYRSDSA VT NFQSS PDGT G T TPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
F+VELEFDAKH PFYLSF+LK+P+ V SG+SEIRSHRKTSF VPVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
SGDGSINFSIFSSTAESVVLCLYNDS SEKP+LELDLDPYINRSGNIWHASFE NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300
Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
YGY+CKG K H NQD +EVSRI+VDPYAKIL SIPK+ QGLG +FLGQ+ VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
WD EVRPNLPMEKLFVYRLNVK FT+DKSSQLP DIAGTFSGLTKKLLH LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
EPIF FDEKEGPYFP HFFSPTN YGPS SISAINSMKEMVKELHANGVEV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
T+VDGALQGIDDSSYYF NRV NLE +SALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE + ETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFC+DIRDFFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
+S LASELSWNCGEEGPT+ +VLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
VAFNDRRSF+WDLLKT FGTQTTQFI FLSSFR++RFDL+QSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWED SCKFLAV+LRADKEEN SITE+ K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSN 885
R++DTAL FPGFFSSDGE VP TGS TYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884
BLAST of Tan0019462 vs. ExPASy TrEMBL
Match:
A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)
HSP 1 Score: 1516.9 bits (3926), Expect = 0.0e+00
Identity = 739/885 (83.50%), Postives = 797/885 (90.06%), Query Frame = 0
Query: 1 MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
MAT LSSLVIQPFCV NCG+TES KLA SDH TYG K KYQ GKMDEARM+A GE+KVG
Sbjct: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
Query: 61 LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
+KSSHRNLSK YA SGISV KSGQR GI GKSKE+ +VATYLFRTEFGDLVNVFV K S
Sbjct: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
FTV +E+ S+QL S+D +LSWGVYRSDSA+VTPNF+SS PD T G T TPFV TSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
F+VELEFDAKH PFYLSF+LKYP+ V SG+SEIRSH+KTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
SGDGS+NFSIFSS+AES+VLCLYNDS SEKP+LELDLDPYINRSGNIWHASFE A FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
YGY+CKGSK H NQD +EVSRI+VDPYAKIL SIPK+ GQGLG S+FLGQ+ KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
WD EV PNLPMEKLFVYRLNV+ FT+DKSSQLP DIAGTFSGLTKKLLHF LGVNAVL+
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
EPIF FDEKEGPYFP HFFSPTN YGPS SISAINSMKEMVKELHANGVEV+LEVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
T+ +GALQGIDDSSYYF NRV NLE KSALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
NAS LLRGHHGELLSRPP VEAIAFDPLLSKTK++ADFWDPQE ETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFC+DIRDFFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
+S LASELSWNCGEEGPTSN +VLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
VAFND+RSF+WDLLKT FGTQTTQFI FLSSFR++RFDL+Q+RNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWED SCKFLAV+LRADKEEN SITE+ K RS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
R++DTAL FPGFFSSDGE VP TGS TYE+QAHSCALFEAKS+ND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885
BLAST of Tan0019462 vs. TAIR 10
Match:
AT1G03310.2 (debranching enzyme 1 )
HSP 1 Score: 950.3 bits (2455), Expect = 1.1e-276
Identity = 479/877 (54.62%), Postives = 626/877 (71.38%), Query Frame = 0
Query: 14 CVCNCGITESFKLAVSDHHT-YGHKIKYQSGKMDEARMVARGESKVGFLKSSHRNLSKAY 73
C N GIT ++K + T +KIK S + R G+ L+S SK
Sbjct: 13 CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQS--YQFSKIC 72
Query: 74 ATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRSGFTVYVEMSSLQ 133
A S S+E ++ + KV +Y FRT+ G LV V VEK R +++ V +SSL+
Sbjct: 73 A-SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLE 132
Query: 134 LTSVD-GKPMLSWGVYRSDSAIVTP-NFQSSLPDGTIGTTATPFVNTSEGNFAVELEFDA 193
L+ D + ++ WGVYRSDS+ P +F++S D TT T FV +S + LEFD
Sbjct: 133 LSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDG 192
Query: 194 KHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSIS-GDGSINFS 253
K PFYLSF LK E+ +HR T FC+PVGF G+P PLGLS D S NFS
Sbjct: 193 KESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFS 252
Query: 254 IFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVSYGYRCKGSK 313
FS ++ +VVLCLY+DS ++KP LELDLDPY+NR+G++WHAS ++ +FV YGYRCK +
Sbjct: 253 FFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETA 312
Query: 314 FHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFDWDREVRPNL 373
+VE I++DPYA ++ S+ + ++LG + K P+FDW +V PN+
Sbjct: 313 HSKEDVDVEGEPIVLDPYATVVGKSV----------SQKYLGSLSKSPSFDWGEDVSPNI 372
Query: 374 PMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLIEPIFPFDEK 433
P+EKL VYRLNVK FT +SS+LP+++AGTFSG+ +K+ H TLG NAVL+EPIF F E+
Sbjct: 373 PLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQ 432
Query: 434 EGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTHTAVDGALQG 493
+GPYFP HFFSP +IYGPS SA+NSMK MVK+LH+ G+EV+LEVVFTHTA GAL+G
Sbjct: 433 KGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRG 492
Query: 494 IDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGH 553
IDDSSYY+ R +L+ KS LNCNYP+VQQL+L+SLRYWVTEFH+DGFCFINASSLLRG
Sbjct: 493 IDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGV 552
Query: 554 HGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNSKFCNDIR 613
HGE LSRPPLVEAIAFDPLL++TK+IAD WDP E + E RFPHWKRWAE+N+++C ++R
Sbjct: 553 HGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVR 612
Query: 614 DFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELS 673
+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN+GL LVD+VSFS +LASELS
Sbjct: 613 NFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELS 672
Query: 674 WNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGSVAFNDRRSF 733
WNCGEEG T+ + VL++RLKQIRNF+F+ ++SLGVPVL+MGDECG S+ GS R+ F
Sbjct: 673 WNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPF 732
Query: 734 DWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSPPQWEDPSCK 793
DW+LL + FGTQ TQFI+F++S RA+R D++Q R+FLK ENI WY N+Q+ P+WEDP+ K
Sbjct: 733 DWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASK 792
Query: 794 FLAVILR--ADKEENGSITEDDKPRS-DIFMAFNASDQSESVALPEPSEGTSWFRLIDTA 853
FLA+ ++ +++EE S+ E ++P+S D+F+ FNASD ESV LP +G+ W RL+DTA
Sbjct: 793 FLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTA 852
Query: 854 LSFPGFFSSDGEPV----PATGSATYEMQAHSCALFE 880
L FPGFFS +GE V P YEM+ +SC LFE
Sbjct: 853 LPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of Tan0019462 vs. TAIR 10
Match:
AT1G03310.1 (debranching enzyme 1 )
HSP 1 Score: 950.3 bits (2455), Expect = 1.1e-276
Identity = 479/877 (54.62%), Postives = 626/877 (71.38%), Query Frame = 0
Query: 14 CVCNCGITESFKLAVSDHHT-YGHKIKYQSGKMDEARMVARGESKVGFLKSSHRNLSKAY 73
C N GIT ++K + T +KIK S + R G+ L+S SK
Sbjct: 13 CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQS--YQFSKIC 72
Query: 74 ATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRSGFTVYVEMSSLQ 133
A S S+E ++ + KV +Y FRT+ G LV V VEK R +++ V +SSL+
Sbjct: 73 A-SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLE 132
Query: 134 LTSVD-GKPMLSWGVYRSDSAIVTP-NFQSSLPDGTIGTTATPFVNTSEGNFAVELEFDA 193
L+ D + ++ WGVYRSDS+ P +F++S D TT T FV +S + LEFD
Sbjct: 133 LSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDG 192
Query: 194 KHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSIS-GDGSINFS 253
K PFYLSF LK E+ +HR T FC+PVGF G+P PLGLS D S NFS
Sbjct: 193 KESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFS 252
Query: 254 IFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVSYGYRCKGSK 313
FS ++ +VVLCLY+DS ++KP LELDLDPY+NR+G++WHAS ++ +FV YGYRCK +
Sbjct: 253 FFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETA 312
Query: 314 FHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFDWDREVRPNL 373
+VE I++DPYA ++ S+ + ++LG + K P+FDW +V PN+
Sbjct: 313 HSKEDVDVEGEPIVLDPYATVVGKSV----------SQKYLGSLSKSPSFDWGEDVSPNI 372
Query: 374 PMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLIEPIFPFDEK 433
P+EKL VYRLNVK FT +SS+LP+++AGTFSG+ +K+ H TLG NAVL+EPIF F E+
Sbjct: 373 PLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQ 432
Query: 434 EGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTHTAVDGALQG 493
+GPYFP HFFSP +IYGPS SA+NSMK MVK+LH+ G+EV+LEVVFTHTA GAL+G
Sbjct: 433 KGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRG 492
Query: 494 IDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGH 553
IDDSSYY+ R +L+ KS LNCNYP+VQQL+L+SLRYWVTEFH+DGFCFINASSLLRG
Sbjct: 493 IDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGV 552
Query: 554 HGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNSKFCNDIR 613
HGE LSRPPLVEAIAFDPLL++TK+IAD WDP E + E RFPHWKRWAE+N+++C ++R
Sbjct: 553 HGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVR 612
Query: 614 DFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELS 673
+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN+GL LVD+VSFS +LASELS
Sbjct: 613 NFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELS 672
Query: 674 WNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGSVAFNDRRSF 733
WNCGEEG T+ + VL++RLKQIRNF+F+ ++SLGVPVL+MGDECG S+ GS R+ F
Sbjct: 673 WNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPF 732
Query: 734 DWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSPPQWEDPSCK 793
DW+LL + FGTQ TQFI+F++S RA+R D++Q R+FLK ENI WY N+Q+ P+WEDP+ K
Sbjct: 733 DWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASK 792
Query: 794 FLAVILR--ADKEENGSITEDDKPRS-DIFMAFNASDQSESVALPEPSEGTSWFRLIDTA 853
FLA+ ++ +++EE S+ E ++P+S D+F+ FNASD ESV LP +G+ W RL+DTA
Sbjct: 793 FLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTA 852
Query: 854 LSFPGFFSSDGEPV----PATGSATYEMQAHSCALFE 880
L FPGFFS +GE V P YEM+ +SC LFE
Sbjct: 853 LPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of Tan0019462 vs. TAIR 10
Match:
AT4G09020.1 (isoamylase 3 )
HSP 1 Score: 383.3 bits (983), Expect = 5.4e-106
Identity = 251/705 (35.60%), Postives = 360/705 (51.06%), Query Frame = 0
Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSKSEKPVLELDLDPYINRSG 290
G SPLG+S D INF++FS A SV LCL D + ++EL LDP +N++G
Sbjct: 88 GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147
Query: 291 NIWHASFES-ALNFVSYGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLG 350
+ WH E LN V YGYR G + S +L+DPYAK++ + G
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207
Query: 351 STSRFLGQV-CKVPAFDWDREVR-PNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSG 410
++F G + FDW + + PN+P + L +Y +NV+ FT D+SS + I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267
Query: 411 LTKKLLHFITLGVNAVLIEPIFPFDEKE--------------GPYFPSHFFSPTNIYGPS 470
+K+ H LG+NAV + P+F FDE E Y +FF+P + Y
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327
Query: 471 LGS-ISAINSMKEMVKELHANGVEVMLEVVFTHT-------AVDGALQGIDDSSYYFV-- 530
G I A KEMVK LH+ G+EV+L+VV+ HT + +GID+ YY +
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387
Query: 531 -NRVVNLEG-KSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGHHGELLSR 590
N+++N G + LNCN+P+V +L+LDSLR+WVTE+H+DGF F AS L R G LS
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447
Query: 591 PPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNSKFCNDIRDFFRGE- 650
PPL+ AIA D +LS+ KIIA+ WD L +FP+W RWAE N + +D+R F +G+
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGLYL-VGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507
Query: 651 GLVSSLATRLCGSGDVFS-DGRGPAFSFNFIARNAGLPLVDLVSFS-----------SSK 710
G+ S ATR+ GS D++ + R P NF+ + G L DLVS++ +
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567
Query: 711 LASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECG-------QS 770
SWNCG EG T +A + R +Q++NF L +S G P++ MGDE G S
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627
Query: 771 SGGSVAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGN 830
G + N+ + + D K ++ I F S + + NFL I W+ +
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHE-----NFLTQGEITWHED 687
Query: 831 NQSPPQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEG 882
N W++ KFLA L D DI++AFNA D +P+P G
Sbjct: 688 N-----WDNSESKFLAFTL-----------HDGIGGRDIYVAFNAHDYFVKALIPQPPPG 747
BLAST of Tan0019462 vs. TAIR 10
Match:
AT2G39930.1 (isoamylase 1 )
HSP 1 Score: 336.7 bits (862), Expect = 5.8e-92
Identity = 229/690 (33.19%), Postives = 338/690 (48.99%), Query Frame = 0
Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCL--YNDSKSEKPVLELDLDPYINRSGNIW 290
G PSP G ++ DG +NFS++S+ + S +CL +D + K E+ LDP NR+G++W
Sbjct: 72 GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131
Query: 291 HASFESALNFVSYGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSR 350
H + YGYR G + S IL+DPYAK A I + LG
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAK---AIISRDEFGVLGPDDN 191
Query: 351 FLGQV-CKVPA----FDWDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGL 410
Q+ C VP FDW+ ++ LP + L +Y ++V+ FT +SS++ + GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251
Query: 411 TKKLLHFITLGVNAVLIEPIFPFDEKE---------------GPYFPSHFFSPTNIYGPS 470
+KL H LG+N + + P F+E E Y FFSP Y +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311
Query: 471 LGSI---SAINSMKEMVKELHANGVEVMLEVVFTHTAVDG------ALQGIDDSSYYFV- 530
+ AIN K +VKE H G+EV+++VV HTA + +G+D+S YY +
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371
Query: 531 --NRVVNLEG-KSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGH------ 590
N G + NCN+P+V+Q +LD LRYWVTE H+DGF F S + R
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431
Query: 591 -------HGELL------SRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKR 650
G+LL S PP+++ I+ DP+L K+IA+ WD + FPHW
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWD-AGGLYQVGMFPHWGI 491
Query: 651 WAEVNSKFCNDIRDFFRG-EGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVD 710
W+E N KF + +R F +G +G + A LCGS +++ GR P S NFI + G L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551
Query: 711 LVSFSS-SKLAS----------ELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGV 770
LV++++ + LA+ SWNCGEEG ++ V R +Q+RNF L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611
Query: 771 PVLHMGDECGQSSGGS----VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLY 830
P+++MGDE G + GG+ N F WD K + +F L FR + L
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRDECESL- 671
Query: 831 QSRNFLKGENIDWYGNNQSPPQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFN 851
+F + + W+G P W + S +F+A L D + +I++AFN
Sbjct: 672 GLNDFPTAKRLQWHGLAPEIPNWSETS-RFVAFSL------------VDSVKKEIYVAFN 731
BLAST of Tan0019462 vs. TAIR 10
Match:
AT3G20440.2 (Alpha amylase family protein )
HSP 1 Score: 58.2 bits (139), Expect = 4.0e-08
Identity = 54/202 (26.73%), Postives = 91/202 (45.05%), Query Frame = 0
Query: 358 AFDWDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLL-HFITLGVN 417
A+ W + +P +P E L +Y + V S P TF TKK+L H G N
Sbjct: 384 AYKW-KYSKPKVP-ESLRIYECH-----VGISGSEPK--VSTFEEFTKKVLPHVKRAGYN 443
Query: 418 AV-LIEPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLE 477
A+ LI D Y ++FF+ ++ YG + K +V E H G+ V L+
Sbjct: 444 AIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTP-------DDFKRLVDEAHGLGLLVFLD 503
Query: 478 VVFTHTAVD-----GALQGIDDSSYYFVNRVVNLE-GKSALNCNYPIVQQLLLDSLRYWV 537
+V ++ A D G +D +++ R + G V L+ +L +W+
Sbjct: 504 IVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWI 563
Query: 538 TEFHIDGFCFINASSLLRGHHG 552
TE+ +DG+ F + +S++ H+G
Sbjct: 564 TEYQVDGYQFHSLASMIYTHNG 569
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q8L735 | 1.6e-275 | 54.62 | Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2 | [more] |
| Q6AU80 | 1.6e-219 | 52.23 | Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=... | [more] |
| Q9M0S5 | 7.7e-105 | 35.60 | Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2 | [more] |
| B9G434 | 5.5e-103 | 34.93 | Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=... | [more] |
| D0TZF0 | 4.2e-95 | 33.29 | Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_038898155.1 | 0.0e+00 | 87.01 | isoamylase 2, chloroplastic [Benincasa hispida] | [more] |
| XP_022150231.1 | 0.0e+00 | 84.63 | isoamylase 2, chloroplastic [Momordica charantia] | [more] |
| XP_008451396.1 | 0.0e+00 | 84.62 | PREDICTED: isoamylase 2, chloroplastic [Cucumis melo] | [more] |
| XP_022953509.1 | 0.0e+00 | 84.86 | isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata] >XP_022953510.1 isoa... | [more] |
| KAA0057598.1 | 0.0e+00 | 84.50 | isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo v... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DAX4 | 0.0e+00 | 84.63 | isoamylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018448 PE=4 ... | [more] |
| A0A1S3BRF8 | 0.0e+00 | 84.62 | isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1 | [more] |
| A0A6J1GPU3 | 0.0e+00 | 84.86 | isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11145... | [more] |
| A0A5A7UR47 | 0.0e+00 | 84.50 | Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 P... | [more] |
| A0A0A0K551 | 0.0e+00 | 83.50 | Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 S... | [more] |
