Tan0019322 (gene) Snake gourd v1

Overview


Name	Tan0019322
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Receptor-like cytosolic serine/threonine-protein kinase RBK1
Location	LG01: 14949309 .. 14953802 (+)
RNA-Seq Expression	Tan0019322
Synteny	Tan0019322

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTTTGTTGGCTTCCTCTGTTCTTCAAAGAATAACCACCTCCTAAAGATATACCCACCCCTTCTTTCTCTTTCATTTTCCTTTTTATTTTTATTTTTATTTTTCTTTTTTATCTCTCTCTCTCTTTTCAATTCTCTGCGAGAGGGAGCTTCCATTTCTGGTCTCCAGATGTAACGGAGGTTCCTTCCCTTTTTTCGTTTGATTTCGACTCATTCTTTTCCAGGACGGAATTCATCTCTCTCTTCTTCTTCTTCTTTTTCTTCTTCTTCTTAATTTCATTCTTCTTGCTGCCTAATTCCAAAACCATGACTGCCAAAGGTAGATGGAATTTCATTTCATTTCATTATCGATTTCTTTTCAATCTTCTTTTAATTCATACATTGCCCTTCTCACCCTCTTGTCGGTAGAGAGGTGTTTTTTGTTTGATTTCTATGTTGTTTTCTTGTCTCCTTCCTGATTTTTGTTTTCCCTCTCTGTTTTTGTTTTTCTCTCTAACCGAATGACTGATTCAGATAATACTACAATTCTAATTTGTTCATCGCATTTCTTTCTTTCTTTCTTTTTTTTTCCTTTTATTGCAGTGGAGACTAAAGATCAAACAGATCAAATTCCAGAAACGGAAATGCAAACGGCACTTGGTAAATTCTGTTATCAAATTGTGTGTGTGTGTGTGTGTTTTTTTTGTTTTTCTCTCGCTAGGTTAGAGAATCGAAATTGAATTTGTAGAATCTCAATTTTGAATCGGTGCTTGGATTGGTGAAAAATCAGGGGAGCCTGGAAAAGGATTAGTAAAGGAAATGGAAGAGACGAAGGGAGAAGGGGAGATGAAAACTAAGGGCGACGAGGAATATCCAAGCGAAGAAATCTCGCCGAGGGGTGTGTTTGAGGTCCCGGTTACGGGCACGGATTCGGACCACAGTGAAACCAGCACCGGCGCCGTCGGTGATGGTGATGGTGACGGTGACGGTGGTGACGGTGGAGATGGAGGGAGCCCAACGGCGGCGGAGAAGTCGCTGTTACCGGCGGTTCTTCTCCAAGAGGCCGGTGGAATTCCATGGAAGACGATGATTGGATCGATAAAGAAGAAATCGGTTTGGAGATTATCGACATTTCCATTACTGGCTGCCAGCTATGATATTTCGAGGAAGAATTTCAAGAGGAAATTGGCTCGGATTCGAAGCGCCGATGATGCCATCAGTAGCGATGATATTCCCTTCTGCAAACCTTCTTGGAGAAACTATGGCTTCTCGGAGCTCGCCACTGCTACCAACAATTTCAGCCCTGGTATCAAACTTCCAAATATTCATCTTTTTTCTTCTTTTATTAAGTTCACGTTCTATACAAAATGTTCTTAAATTCACACGAAATTGAATTTTAAAATAGGTATAATATGTCATGCACAAAATTAAAATTTTAAACATCTATAACACTATTTTTAAGTTTAGAAATTTATTAAAAATACAGAGGATGAATTAGACACGGACACCGTAAATTTTTGGTCTAAATTGTAATTTAACTTTTTTTTCCCCTTCTTTTTCTAACTATTTTATCCCTAATCGTTGAATCATATAATATTGTTTCTGTTTTTTCTATTTTCCCGCGAGTCATGGCAGATTGTCGTTGGCTTGTTCTAATTTTTCTGACAAGTTTTTTAGTTCCATGATATCTGTCCCATATCGGAGATTTGAGTGAGTGAGACCCACCCTAGACTTTCTTTTTTCTCTTTCACTAAAATATTCAATAATTTATTCATTTAAATTCTGTGTTTAGGGATGATTTAATACGAAATTGGAGTATAGGATCTTGATTACAAATCAATAGTGACAGTTACTTGTTGGGTTTCGTTCAAATTTTTGTTCTTGTTGATTTTTTTTTTTTTTTTTTAAAAAAATGACAAGTGGAGGTATTTTATGTAGTTATCGATTTTTTTACTCGGTGTTTCTTATCATCTATCAAACTTTTTTTTTTTTGTCAAGGTTAAGAAAACCAAATATACCATGAGAAAAATTCTTGTGAATCAGTGATTTGGTAGGTCTCGTGCCCACAAAGGAAGAGCTTTTAAGAATGCATTAAAAATTTTGAAGAAAATGGGATTTTGCAACCTTAATTATTCGACTCTTGGTTCTAAGTCTCTTTCAGAATCCCAATATGTAAGAAGAAACGTTATAGTGAGTGAATTTTGAATTCATAGGCTTTCAAACGCTTGCGATTATATTAACTGTTTTTCATAGTCTTTCATAGGTATTTTTCTATTAGAAGTTTGAATAATTTATATAGGTAGGTTGAATAGATATAATAATTAATTAGTAAATTTAGTTGGTATTTTAACTGAAACTTGGTCAGCAGCCTGTCATAATATGTAGCTTTGAAAGTCAATGGCGTCGCGGGACTTTTCATTGAGTAGTCAATTTCAATTCATAAATATAAAAGTTCAGAAGTTAGGCCCCTTCTTTTTTCAGTATTTGCAGCCTTTCATAATCAACTAAAACCCAACCACCTTTCACATTGAATTAGACATTTGTGTTTGGGTTGCCTTTATGGTTCAAAATTTCTAATCATATGATTGAAACTGTGTTCAGAAAACTTGCTTGGAAAAGGAGGGCAAGCAGAGGTTTACAAAGGTTGCTTATCCGATGGCCAAGTTGTGGCGGTTAAGAGACTTATGAAAAAGGAGAAGGAAAACGAGGAAAGAACTGCTGATTTTTTATCAGAACTTGGAATTATCGCTCATATAAATCACCCGAATGCTGCACGGTTGCTCGGGTTCGGAATTGATGGTGGTCTCTACCTGGTCCTTGAATACATTCCTTATGGCAGCTTTGCCTCTGCCCTTTTCGGTTCGTCACTCTCCCATAAGTATCATATTGGATCTCATTAATATTTGAATGAACCAATTAGCCCTCTTCTAGCAATGCCCATTTCAACTCTAGTAGTGAGTTGAAATAAACACACATTCGAAAAGCACGGTTTGAATCGCATTGGATGAGAAAACAGAGTTTTTTTTTTTTTAAAACGAATCCGAATTACAATGATATTGAAGGAAAAGATGGATATGTTTGGCTGATGAACGTAATACTTTCGATCAGGTGCGGAATCTCTGGAATGGAAGATAAGATTTAAGGTGGCTGTTGGTGTAGCAGAAGGACTGAACTATCTACATCGTGAATGTCCAAGGCGCATCATTCACAGAGATATCAAAGCCTCTAACATCTTGCTCGCTGAAAACTATGATCCATTGGTAATGTTTTTAGCTGAAGCATTGAGTTTCAGACATTTTTTTCTATTATAATGAATGGTTTTTCTTTCCTGGCGTGCAGATCTCTGATTTTGGATTAGCAAAATGGCTGCCTGAAAATTGGGCTCACCATGTCGTCTTCCCCATCGAGGGTACATTCGGGTGAGTTATACCGACTCATTGAATCGATTCGATTCGATTTGATTTTAACAATCGTATCAAGAACTATGAGATGACTCCTAACACATCTTTAAATCGCCTCAGATATTTGGCTCCAGAGTACTTTATGCATGGAATCGTTGACGAGAAGACTGATGTTTTCGCTTATGGTGTTTTGTTGCTTGAGCTCATAACTGGGCGCCGTGCAGTTGATTCGAGTCGACAAAGCCTTGTGATTTGGGTATGATTTGTAGCTCCATAGCTTAAGTGGTATCCATACAAATTCTTATCATCTTTTAGTATCTGAAGTTAGTCTTGATGAATAATTCCTAGGATGTAGTAACTGCTGCTGCTATTTGTTGTATGCTTAGAAAAGGTTTTGTGTTTTTTGCAGGCAAAGCCATTACTTGATAACAACAGCTTCAAGGAATTGACAGATCCAAAATTGGGAGATGACTACGATCAAACTGAAATGAGACGAACCATGTTAGCAGCTTCAATGTGCATCAACCATTCATCACCGATACGTCCACATATGAACCGGGTAAGGGACCGTGTCTCTTCGATCTCCATTGTTCTCACTTTGTGTGTCTGTTTTGGGTGACTTCAGTGTTGTGGGAGTGTAGGTAGTACAGCTGCTGAAGGGAGAAGATGGACCATTAGATCAATTAAAACAGAAATCAATGGACGGGGATCGATCGCTGCTTTTGGAAGCCTGCGACCTCGAAGATTACACGTGTAATTCGAACTACCTCGACGACCTCAGTCGTCACAGGGAGCTCGTCCTGGAGTGAAGTGTTATGTTTGTGTGCTTCTGCTATTCCCAGAGAAAAACTTGCTTGTTTGTGTTCAAAAGTTATATAAAATGATGCCTATGCAATTGTGTCAAAGGCTGATATAATGTTGTTGTTCACACAAATCAGCTGTGTCCCTTTCTATGGCTGTGCCTGTGTTATATACAAATACAACCTTGAAAGGCTCTTCTTTTTGTCAAAAATATAGGGATGATAGAAGGGCCATTTATATATATAAATATACATTTATTTATTTGCATTTTCCGAGGCTAAAAAGATACACGGAAATTGTATATTGCATTATAA

mRNA sequence

GGTTTTGTTGGCTTCCTCTGTTCTTCAAAGAATAACCACCTCCTAAAGATATACCCACCCCTTCTTTCTCTTTCATTTTCCTTTTTATTTTTATTTTTATTTTTCTTTTTTATCTCTCTCTCTCTTTTCAATTCTCTGCGAGAGGGAGCTTCCATTTCTGGTCTCCAGATGTAACGGAGGTTCCTTCCCTTTTTTCGTTTGATTTCGACTCATTCTTTTCCAGGACGGAATTCATCTCTCTCTTCTTCTTCTTCTTTTTCTTCTTCTTCTTAATTTCATTCTTCTTGCTGCCTAATTCCAAAACCATGACTGCCAAAGTGGAGACTAAAGATCAAACAGATCAAATTCCAGAAACGGAAATGCAAACGGCACTTGGGGAGCCTGGAAAAGGATTAGTAAAGGAAATGGAAGAGACGAAGGGAGAAGGGGAGATGAAAACTAAGGGCGACGAGGAATATCCAAGCGAAGAAATCTCGCCGAGGGGTGTGTTTGAGGTCCCGGTTACGGGCACGGATTCGGACCACAGTGAAACCAGCACCGGCGCCGTCGGTGATGGTGATGGTGACGGTGACGGTGGTGACGGTGGAGATGGAGGGAGCCCAACGGCGGCGGAGAAGTCGCTGTTACCGGCGGTTCTTCTCCAAGAGGCCGGTGGAATTCCATGGAAGACGATGATTGGATCGATAAAGAAGAAATCGGTTTGGAGATTATCGACATTTCCATTACTGGCTGCCAGCTATGATATTTCGAGGAAGAATTTCAAGAGGAAATTGGCTCGGATTCGAAGCGCCGATGATGCCATCAGTAGCGATGATATTCCCTTCTGCAAACCTTCTTGGAGAAACTATGGCTTCTCGGAGCTCGCCACTGCTACCAACAATTTCAGCCCTGAAAACTTGCTTGGAAAAGGAGGGCAAGCAGAGGTTTACAAAGGTTGCTTATCCGATGGCCAAGTTGTGGCGGTTAAGAGACTTATGAAAAAGGAGAAGGAAAACGAGGAAAGAACTGCTGATTTTTTATCAGAACTTGGAATTATCGCTCATATAAATCACCCGAATGCTGCACGGTTGCTCGGGTTCGGAATTGATGGTGGTCTCTACCTGGTCCTTGAATACATTCCTTATGGCAGCTTTGCCTCTGCCCTTTTCGGTGCGGAATCTCTGGAATGGAAGATAAGATTTAAGGTGGCTGTTGGTGTAGCAGAAGGACTGAACTATCTACATCGTGAATGTCCAAGGCGCATCATTCACAGAGATATCAAAGCCTCTAACATCTTGCTCGCTGAAAACTATGATCCATTGATCTCTGATTTTGGATTAGCAAAATGGCTGCCTGAAAATTGGGCTCACCATGTCGTCTTCCCCATCGAGGGTACATTCGGATATTTGGCTCCAGAGTACTTTATGCATGGAATCGTTGACGAGAAGACTGATGTTTTCGCTTATGGTGTTTTGTTGCTTGAGCTCATAACTGGGCGCCGTGCAGTTGATTCGAGTCGACAAAGCCTTGTGATTTGGGCAAAGCCATTACTTGATAACAACAGCTTCAAGGAATTGACAGATCCAAAATTGGGAGATGACTACGATCAAACTGAAATGAGACGAACCATGTTAGCAGCTTCAATGTGCATCAACCATTCATCACCGATACGTCCACATATGAACCGGGTAGTACAGCTGCTGAAGGGAGAAGATGGACCATTAGATCAATTAAAACAGAAATCAATGGACGGGGATCGATCGCTGCTTTTGGAAGCCTGCGACCTCGAAGATTACACGTGTAATTCGAACTACCTCGACGACCTCAGTCGTCACAGGGAGCTCGTCCTGGAGTGAAGTGTTATGTTTGTGTGCTTCTGCTATTCCCAGAGAAAAACTTGCTTGTTTGTGTTCAAAAGTTATATAAAATGATGCCTATGCAATTGTGTCAAAGGCTGATATAATGTTGTTGTTCACACAAATCAGCTGTGTCCCTTTCTATGGCTGTGCCTGTGTTATATACAAATACAACCTTGAAAGGCTCTTCTTTTTGTCAAAAATATAGGGATGATAGAAGGGCCATTTATATATATAAATATACATTTATTTATTTGCATTTTCCGAGGCTAAAAAGATACACGGAAATTGTATATTGCATTATAA

Coding sequence (CDS)

ATGACTGCCAAAGTGGAGACTAAAGATCAAACAGATCAAATTCCAGAAACGGAAATGCAAACGGCACTTGGGGAGCCTGGAAAAGGATTAGTAAAGGAAATGGAAGAGACGAAGGGAGAAGGGGAGATGAAAACTAAGGGCGACGAGGAATATCCAAGCGAAGAAATCTCGCCGAGGGGTGTGTTTGAGGTCCCGGTTACGGGCACGGATTCGGACCACAGTGAAACCAGCACCGGCGCCGTCGGTGATGGTGATGGTGACGGTGACGGTGGTGACGGTGGAGATGGAGGGAGCCCAACGGCGGCGGAGAAGTCGCTGTTACCGGCGGTTCTTCTCCAAGAGGCCGGTGGAATTCCATGGAAGACGATGATTGGATCGATAAAGAAGAAATCGGTTTGGAGATTATCGACATTTCCATTACTGGCTGCCAGCTATGATATTTCGAGGAAGAATTTCAAGAGGAAATTGGCTCGGATTCGAAGCGCCGATGATGCCATCAGTAGCGATGATATTCCCTTCTGCAAACCTTCTTGGAGAAACTATGGCTTCTCGGAGCTCGCCACTGCTACCAACAATTTCAGCCCTGAAAACTTGCTTGGAAAAGGAGGGCAAGCAGAGGTTTACAAAGGTTGCTTATCCGATGGCCAAGTTGTGGCGGTTAAGAGACTTATGAAAAAGGAGAAGGAAAACGAGGAAAGAACTGCTGATTTTTTATCAGAACTTGGAATTATCGCTCATATAAATCACCCGAATGCTGCACGGTTGCTCGGGTTCGGAATTGATGGTGGTCTCTACCTGGTCCTTGAATACATTCCTTATGGCAGCTTTGCCTCTGCCCTTTTCGGTGCGGAATCTCTGGAATGGAAGATAAGATTTAAGGTGGCTGTTGGTGTAGCAGAAGGACTGAACTATCTACATCGTGAATGTCCAAGGCGCATCATTCACAGAGATATCAAAGCCTCTAACATCTTGCTCGCTGAAAACTATGATCCATTGATCTCTGATTTTGGATTAGCAAAATGGCTGCCTGAAAATTGGGCTCACCATGTCGTCTTCCCCATCGAGGGTACATTCGGATATTTGGCTCCAGAGTACTTTATGCATGGAATCGTTGACGAGAAGACTGATGTTTTCGCTTATGGTGTTTTGTTGCTTGAGCTCATAACTGGGCGCCGTGCAGTTGATTCGAGTCGACAAAGCCTTGTGATTTGGGCAAAGCCATTACTTGATAACAACAGCTTCAAGGAATTGACAGATCCAAAATTGGGAGATGACTACGATCAAACTGAAATGAGACGAACCATGTTAGCAGCTTCAATGTGCATCAACCATTCATCACCGATACGTCCACATATGAACCGGGTAGTACAGCTGCTGAAGGGAGAAGATGGACCATTAGATCAATTAAAACAGAAATCAATGGACGGGGATCGATCGCTGCTTTTGGAAGCCTGCGACCTCGAAGATTACACGTGTAATTCGAACTACCTCGACGACCTCAGTCGTCACAGGGAGCTCGTCCTGGAGTGA

Protein sequence

MTAKVETKDQTDQIPETEMQTALGEPGKGLVKEMEETKGEGEMKTKGDEEYPSEEISPRGVFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGDGGSPTAAEKSLLPAVLLQEAGGIPWKTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWRNYGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRSLLLEACDLEDYTCNSNYLDDLSRHRELVLE

Homology

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BLAST of Tan0019322 vs. ExPASy Swiss-Prot
Match: Q8H1D6 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thaliana OX=3702 GN=RBK1 PE=1 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 3.0e-146
Identity = 268/476 (56.30%), Postives = 350/476 (73.53%), Query Frame = 0


Query: 36  ETKGEGEMKTKGDEEYPSEEISPRGVFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGD 95
           E+K   E++   ++    +  SPRGV  +    +DSD+S +S  +              D
Sbjct: 10  ESKNHQEVELHRNDLGLEDSSSPRGVLGMV---SDSDNSSSSCSSC----------SSDD 69

Query: 96  GGSPTAAEKSLLPAVLLQEAGGIPWKTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRK 155
             S T++  S     +     G+ W  MI SIKKKS+ R S  PLL ASY+++RKN +RK
Sbjct: 70  KSSSTSSPFSNTTKTVSSSHHGLQWNKMIESIKKKSMRRFSVIPLL-ASYELTRKNLRRK 129

Query: 156 LARIRSADDAISSDDIPFCKPSWRNYGFSELATATNNFSPENLLGKGGQAEVYKGCLSDG 215
             ++  ++ A + +     KPSWRN+ + ELA AT+ F+PEN++GKGG AEVYKG L +G
Sbjct: 130 QPKLTPSESAFTCEAFFMAKPSWRNFTYEELAVATDYFNPENMIGKGGHAEVYKGVLING 189

Query: 216 QVVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGS 275
           + VA+K+LM   KE EER +DFLSELGIIAH+NHPNAARL GF  D GL+ VLEY PYGS
Sbjct: 190 ETVAIKKLMSHAKEEEERVSDFLSELGIIAHVNHPNAARLRGFSSDRGLHFVLEYAPYGS 249

Query: 276 FASALFGAES-LEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENYDPLIS 335
            AS LFG+E  LEWKIR+KVA+G+A+GL+YLH  CPRRIIHRDIKASNILL  +Y+  IS
Sbjct: 250 LASMLFGSEECLEWKIRYKVALGIADGLSYLHNACPRRIIHRDIKASNILLNHDYEAQIS 309

Query: 336 DFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAV 395
           DFGLAKWLPENW HHVVFPIEGTFGYLAPEYFMHGIVDEK DVFA+GVLLLE+IT RRAV
Sbjct: 310 DFGLAKWLPENWPHHVVFPIEGTFGYLAPEYFMHGIVDEKIDVFAFGVLLLEIITSRRAV 369

Query: 396 D-SSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMN 455
           D +SRQS+V WAKP L+ NS +++ DP+LG+ ++ TEM+R ML ASMC++H + +RP M 
Sbjct: 370 DTASRQSIVAWAKPFLEKNSMEDIVDPRLGNMFNPTEMQRVMLTASMCVHHIAAMRPDMT 429

Query: 456 RVVQLLKGEDGPLDQLKQKSMDGDRSLLLEACDLEDYTCNSNYLDDLSRHRELVLE 510
           R+VQLL+GEDGP  +L+QK+  G+R++ + ACDL+D+T +S+YL++L RHR+L++E
Sbjct: 430 RLVQLLRGEDGPA-ELQQKA--GERTMSVNACDLQDHT-SSSYLNELRRHRQLLME 467

[Close]

BLAST of Tan0019322 vs. ExPASy Swiss-Prot
Match: Q8RXC8 (Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thaliana OX=3702 GN=RBK2 PE=1 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 3.0e-101
Identity = 201/396 (50.76%), Postives = 265/396 (66.92%), Query Frame = 0


Query: 120 WKTMIGSIKKKSVWRLSTF-PLLAASYDISRKNFKRKLARIRSADDAISS---DDIPFCK 179
           W       K++S+ RLS+F PL  A+  I ++N        +SAD  ++     DI   +
Sbjct: 74  WGKFFKLWKRRSMKRLSSFPPLSGAAPPIIKQN--------KSADPNMNGMVLHDIYDFQ 133

Query: 180 PSWRNYGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTA 239
            S +N+  S++  AT+NFSPEN++G+GG A+VY+G L +G+++AVKRL K     +E+TA
Sbjct: 134 SSLQNFSISDIEIATDNFSPENIIGRGGYADVYQGILPEGKLIAVKRLTKGTP--DEQTA 193

Query: 240 DFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAE--SLEWKIRFK 299
           +FLSELGIIAH++HPN A+ +G  I+GG++LV    P GS  S L G     L W  R+ 
Sbjct: 194 EFLSELGIIAHVDHPNTAKFIGCCIEGGMHLVFRLSPLGSLGSLLHGPSKYKLTWSRRYN 253

Query: 300 VAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFP 359
           VA+G A+GL YLH  C RRIIHRDIKA NILL E++ P I DFGLAKWLP+   HH V  
Sbjct: 254 VALGTADGLVYLHEGCQRRIIHRDIKADNILLTEDFQPQICDFGLAKWLPKQLTHHNVSK 313

Query: 360 IEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNS 419
            EGTFGY APEYFMHGIVDEKTDVFA+GVLLLELITG  A+D S+QSLV+WAKPLL+  +
Sbjct: 314 FEGTFGYFAPEYFMHGIVDEKTDVFAFGVLLLELITGHPALDESQQSLVLWAKPLLERKA 373

Query: 420 FKELTDPKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKG-EDGPLDQLKQK 479
            KEL DP LGD+Y++ E+ R    AS+CI+ SS +RP M++VV+LL G ED  +   + K
Sbjct: 374 IKELVDPSLGDEYNREELIRLTSTASLCIDQSSLLRPRMSQVVELLLGHEDVVMTPREAK 433

Query: 480 SMDGDRSLLLEACDLEDYTCNSNYLDDLSRHRELVL 509
                R+   E  D  +Y  ++ YL DL R RE+ L
Sbjct: 434 IKMMQRTYSEELLDSVEYN-STKYLGDLDRIREVAL 458

[Close]

BLAST of Tan0019322 vs. ExPASy Swiss-Prot
Match: Q5XF57 (Probable receptor-like serine/threonine-protein kinase At5g57670 OS=Arabidopsis thaliana OX=3702 GN=At5g57670 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 7.8e-86
Identity = 149/290 (51.38%), Postives = 210/290 (72.41%), Query Frame = 0


Query: 172 PFCKPSWRNYGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENE 231
           P  +P  + + ++E++ ATN+F   N++G GG +EVY+G L DG+ +AVKRL  KE  + 
Sbjct: 246 PKPQPLIQCFTYNEISKATNDFHQGNIVGIGGYSEVYRGDLWDGRRIAVKRL-AKESGDM 305

Query: 232 ERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAE--SLEWK 291
            +  +FL+ELGII+H++HPN A LLG  ++ GLYLV  +   G+  SAL   E  SL+W 
Sbjct: 306 NKEKEFLTELGIISHVSHPNTALLLGCCVEKGLYLVFRFSENGTLYSALHENENGSLDWP 365

Query: 292 IRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHH 351
           +R+K+AVGVA GL+YLH+ C  RIIHRDIK+SN+LL  +Y+P I+DFGLAKWLP  W HH
Sbjct: 366 VRYKIAVGVARGLHYLHKRCNHRIIHRDIKSSNVLLGPDYEPQITDFGLAKWLPNKWTHH 425

Query: 352 VVFPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLL 411
            V P+EGTFGYLAPE  M G +DEKTD++A+G+LLLE+ITGRR V+ +++ +++WAKP +
Sbjct: 426 AVIPVEGTFGYLAPESLMQGTIDEKTDIYAFGILLLEIITGRRPVNPTQKHILLWAKPAM 485

Query: 412 DNNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLL 460
           +  +  EL DPKL D YD  +M + +L AS C+  S  +RP M +V++LL
Sbjct: 486 ETGNTSELVDPKLQDKYDDQQMNKLVLTASHCVQQSPILRPTMTQVLELL 534

[Close]

BLAST of Tan0019322 vs. ExPASy Swiss-Prot
Match: Q9LV48 (Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 GN=PERK1 PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 5.4e-63
Identity = 140/339 (41.30%), Postives = 208/339 (61.36%), Query Frame = 0


Query: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLSE 240
           + + EL+ ATN FS  NLLG+GG   V+KG L  G+ VAVK+L     + E    +F +E
Sbjct: 268 FTYEELSRATNGFSEANLLGQGGFGYVHKGILPSGKEVAVKQLKAGSGQGER---EFQAE 327

Query: 241 LGIIAHINHPNAARLLGFGIDG-GLYLVLEYIPYGSFASALF--GAESLEWKIRFKVAVG 300
           + II+ ++H +   L+G+ + G    LV E++P  +    L   G  ++EW  R K+A+G
Sbjct: 328 VEIISRVHHRHLVSLIGYCMAGVQRLLVYEFVPNNNLEFHLHGKGRPTMEWSTRLKIALG 387

Query: 301 VAEGLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGT 360
            A+GL+YLH +C  +IIHRDIKASNIL+   ++  ++DFGLAK +  +   HV   + GT
Sbjct: 388 SAKGLSYLHEDCNPKIIHRDIKASNILIDFKFEAKVADFGLAK-IASDTNTHVSTRVMGT 447

Query: 361 FGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSR----QSLVIWAKPLLDNNS 420
           FGYLAPEY   G + EK+DVF++GV+LLELITGRR VD++      SLV WA+PLL+  S
Sbjct: 448 FGYLAPEYAASGKLTEKSDVFSFGVVLLELITGRRPVDANNVYVDDSLVDWARPLLNRAS 507

Query: 421 ----FKELTDPKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQL 480
               F+ L D K+G++YD+ EM R +  A+ C+ HS+  RP M+++V+ L+G +  L  L
Sbjct: 508 EEGDFEGLADSKMGNEYDREEMARMVACAAACVRHSARRRPRMSQIVRALEG-NVSLSDL 567

Query: 481 KQKSMDGDRSLLLEACDLEDYTCNSNYLDDLSRHRELVL 509
            +    G  ++        DY   S Y DD+ + R++ L
Sbjct: 568 NEGMRPGHSNVYSSYGGSTDYD-TSQYNDDMIKFRKMAL 600

[Close]

BLAST of Tan0019322 vs. ExPASy Swiss-Prot
Match: Q9LK03 (Proline-rich receptor-like protein kinase PERK2 OS=Arabidopsis thaliana OX=3702 GN=PERK2 PE=2 SV=3)

HSP 1 Score: 242.7 bits (618), Expect = 9.3e-63
Identity = 139/340 (40.88%), Postives = 210/340 (61.76%), Query Frame = 0


Query: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLSE 240
           + + EL+ ATN FS  NLLG+GG   V+KG L +G+ VAVK+L +   + E    +F +E
Sbjct: 342 FNYEELSRATNGFSEANLLGQGGFGYVFKGMLRNGKEVAVKQLKEGSSQGER---EFQAE 401

Query: 241 LGIIAHINHPNAARLLGFGI-DGGLYLVLEYIPYGSFASALF--GAESLEWKIRFKVAVG 300
           +GII+ ++H +   L+G+ I D    LV E++P  +    L   G  ++EW  R K+AVG
Sbjct: 402 VGIISRVHHRHLVALVGYCIADAQRLLVYEFVPNNTLEFHLHGKGRPTMEWSSRLKIAVG 461

Query: 301 VAEGLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGT 360
            A+GL+YLH  C  +IIHRDIKASNIL+   ++  ++DFGLAK +  +   HV   + GT
Sbjct: 462 SAKGLSYLHENCNPKIIHRDIKASNILIDFKFEAKVADFGLAK-IASDTNTHVSTRVMGT 521

Query: 361 FGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSR----QSLVIWAKPLLDN-- 420
           FGYLAPEY   G + EK+DVF++GV+LLELITGRR +D +      SLV WA+PLL+   
Sbjct: 522 FGYLAPEYASSGKLTEKSDVFSFGVVLLELITGRRPIDVNNVHADNSLVDWARPLLNQVS 581

Query: 421 --NSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQL 480
              +F+ + D KL ++YD+ EM R +  A+ C+  ++P RP M++V ++L+G   P D L
Sbjct: 582 ELGNFEVVVDKKLNNEYDKEEMARMVACAAACVRSTAPRRPRMDQVARVLEGNISPSD-L 641

Query: 481 KQKSMDGDRSLLLEACDLEDYTCNSNYLDDLSRHRELVLE 510
            Q    G  ++   +    DY  +S   + +++ R++ LE
Sbjct: 642 NQGITPGHSNVYGSSGGSTDYD-SSQDNEGMNKFRKVGLE 675

[Close]

BLAST of Tan0019322 vs. NCBI nr
Match: KAA0060424.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucumis melo var. makuwa])

HSP 1 Score: 847.8 bits (2189), Expect = 4.8e-242
Identity = 429/510 (84.12%), Postives = 460/510 (90.20%), Query Frame = 0


Query: 1   MTAKVETKDQTDQIPETEMQTALGEPGKGLVKEMEET-KGEGEMKTKGDEEYPSEEISPR 60
           MT +VE KDQTDQIP+ EM+  +GE GK   KEMEET +GE  MK KG+EE+  +E SPR
Sbjct: 1   MTVQVEIKDQTDQIPQPEME--IGEIGK-RKKEMEETEEGEEGMKVKGEEEFVCDENSPR 60

Query: 61  GVFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGDGGSPTAAEKSLLPAVLLQEAGGIP 120
           GVFE+P+ GTDSD SE+S+G  GD +           G+ +  EK+LLPA LLQE GGIP
Sbjct: 61  GVFEIPIVGTDSDQSESSSGGGGDVE-----------GTLSVPEKALLPAALLQEVGGIP 120

Query: 121 WKTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWR 180
           WK MIG IKKKSVWR ST PLLAASY+ISRKNFKRKLARIRSAD+ I+SDDIPFCKPSWR
Sbjct: 121 WKAMIGCIKKKSVWRFSTIPLLAASYEISRKNFKRKLARIRSADEGITSDDIPFCKPSWR 180

Query: 181 NYGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLS 240
           NYGFSEL+ ATNNFSPENLLGKGGQAEVYKGCLSDGQ+VAVKRLMKKEKENEERTADFLS
Sbjct: 181 NYGFSELSAATNNFSPENLLGKGGQAEVYKGCLSDGQIVAVKRLMKKEKENEERTADFLS 240

Query: 241 ELGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAESLEWKIRFKVAVGVA 300
           ELGIIAHINHPNAARLLGFGI+ GLYLVLE+IP+GSFASALFGAESLEW IRFKVA+GVA
Sbjct: 241 ELGIIAHINHPNAARLLGFGIESGLYLVLEFIPHGSFASALFGAESLEWNIRFKVALGVA 300

Query: 301 EGLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFG 360
           EGLNYLH ECPRRIIHRDIKASNILLAENY+PLISDFGLAKWLPENWAHHVVFPIEGTFG
Sbjct: 301 EGLNYLHCECPRRIIHRDIKASNILLAENYEPLISDFGLAKWLPENWAHHVVFPIEGTFG 360

Query: 361 YLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTD 420
           YLAPEYFMHGIVDEKTDVFA+GVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTD
Sbjct: 361 YLAPEYFMHGIVDEKTDVFAFGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTD 420

Query: 421 PKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRS 480
           PKLGDDYDQTEMRRTMLAASMCINHSS +RPHMNRVVQLLKGEDGPLDQLK KSMDGDRS
Sbjct: 421 PKLGDDYDQTEMRRTMLAASMCINHSSSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRS 480

Query: 481 LLLEACDLEDYTCNSNYLDDLSRHRELVLE 510
            LLEACDLEDYT N++YL+DLSRHRELVLE
Sbjct: 481 FLLEACDLEDYTNNTSYLNDLSRHRELVLE 496

[Close]

BLAST of Tan0019322 vs. NCBI nr
Match: KAG6577338.1 (Receptor-like cytosolic serine/threonine-protein kinase RBK1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 847.4 bits (2188), Expect = 6.3e-242
Identity = 423/508 (83.27%), Postives = 456/508 (89.76%), Query Frame = 0


Query: 1   MTAKVETKDQTDQIPETEMQTALGEPGKGLVKEMEETKGEGEMKTKGDEEYPSEEISPRG 60
           MT  VE K+Q ++  E EM+    E    L KEM+E  GEG MK KG E YP +E SPRG
Sbjct: 1   MTVPVEIKNQLEKFLEMEMEM---EMEMELGKEMDEMGGEGGMKIKGVERYPCDENSPRG 60

Query: 61  VFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGDGGSPTAAEKSLLPAVLLQEAGGIPW 120
           VFE+P+ GTDSD SE+S+G          GGDG  GGSPTAAEK+L+PA LLQE GGIPW
Sbjct: 61  VFEIPIMGTDSDQSESSSGG---------GGDGEAGGSPTAAEKALIPAALLQEIGGIPW 120

Query: 121 KTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWRN 180
           +TMIGSIKKK++WR STFPLLAA Y++SRKNFKRKLARIRSAD+ I+SDDIPFCKPSWRN
Sbjct: 121 RTMIGSIKKKTIWRFSTFPLLAAGYELSRKNFKRKLARIRSADEGITSDDIPFCKPSWRN 180

Query: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLSE 240
           YGFSELATATNNFSPENLLGKGGQAEVYKGC +DGQVVAVKRLMKKEKENEERTADFLSE
Sbjct: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCFADGQVVAVKRLMKKEKENEERTADFLSE 240

Query: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAESLEWKIRFKVAVGVAE 300
           LGIIAHINHPNAARLLGFGIDGGLYLVLE++PYGSFAS+LFGAESL WKIRFKVAVGVAE
Sbjct: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEFVPYGSFASSLFGAESLPWKIRFKVAVGVAE 300

Query: 301 GLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360
           GLNYLHRECPRRIIHRDIKASNILL ENY+PLISDFGLAKWLPENWAHHVVFPIEGTFGY
Sbjct: 301 GLNYLHRECPRRIIHRDIKASNILLDENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360

Query: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDP 420
           LAPEYFMHGIVDEKTDVFAYGVLLLELITGR AVDSSRQSLVIWAKPLLD++SFKELTDP
Sbjct: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRHAVDSSRQSLVIWAKPLLDSSSFKELTDP 420

Query: 421 KLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRSL 480
           KLGDDYDQTEM+RTMLAASMCINH+S +RPHMNRVVQLLKGEDGP+DQLKQKS++GDRSL
Sbjct: 421 KLGDDYDQTEMKRTMLAASMCINHTSSLRPHMNRVVQLLKGEDGPVDQLKQKSLNGDRSL 480

Query: 481 LLEACDLEDYTCNSNYLDDLSRHRELVL 509
           LLEACDLEDYT NS YL DL+RHREL L
Sbjct: 481 LLEACDLEDYTINSTYLSDLTRHRELAL 496

[Close]

BLAST of Tan0019322 vs. NCBI nr
Match: XP_023552649.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 846.3 bits (2185), Expect = 1.4e-241
Identity = 422/508 (83.07%), Postives = 455/508 (89.57%), Query Frame = 0


Query: 1   MTAKVETKDQTDQIPETEMQTALGEPGKGLVKEMEETKGEGEMKTKGDEEYPSEEISPRG 60
           MT  VE K+Q ++  E EM+  +      L KEMEE  GEG MK KG E YP +E SPRG
Sbjct: 1   MTVPVEIKNQLEKFLEMEMEMEM-----ELGKEMEEMGGEGGMKIKGVEGYPCDENSPRG 60

Query: 61  VFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGDGGSPTAAEKSLLPAVLLQEAGGIPW 120
           VFE+P+ GTDSD SE+S+G          GGDG  GGSPTAAEK+L+PA LLQE GGIPW
Sbjct: 61  VFEIPIMGTDSDQSESSSGG---------GGDGDAGGSPTAAEKALIPAALLQEIGGIPW 120

Query: 121 KTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWRN 180
           +TMIGSIKKK++WR STFPLLAA Y++SRKNFKRKLARIRSAD+ I+SDDIPFCKPSWRN
Sbjct: 121 RTMIGSIKKKTIWRFSTFPLLAAGYELSRKNFKRKLARIRSADEGITSDDIPFCKPSWRN 180

Query: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLSE 240
           YGFSELA ATNNFSPENLLGKGGQAEVYKGC +DGQVVAVKRLMKKEKENEERTADFLSE
Sbjct: 181 YGFSELAIATNNFSPENLLGKGGQAEVYKGCFADGQVVAVKRLMKKEKENEERTADFLSE 240

Query: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAESLEWKIRFKVAVGVAE 300
           LGIIAHINHPNAARLLGFGIDGGLYLVLE++PYGSFAS+LFGAESL WKIRFKVAVGVAE
Sbjct: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEFVPYGSFASSLFGAESLPWKIRFKVAVGVAE 300

Query: 301 GLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360
           GLNYLHRECPRRIIHRDIKASNILL ENY+PLISDFGLAKWLPENWAHHVVFPIEGTFGY
Sbjct: 301 GLNYLHRECPRRIIHRDIKASNILLDENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360

Query: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDP 420
           LAPEYFMHGIVDEKTDVFAYGVLLLELITGR AVDSSRQSLVIWAKPLLD++SFKELTDP
Sbjct: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRHAVDSSRQSLVIWAKPLLDSSSFKELTDP 420

Query: 421 KLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRSL 480
           KLGDDYDQTEM+RTMLAASMCINH+S +RPHMNRVVQLLKGEDGP+DQLKQKS++GDRSL
Sbjct: 421 KLGDDYDQTEMKRTMLAASMCINHTSSLRPHMNRVVQLLKGEDGPVDQLKQKSLNGDRSL 480

Query: 481 LLEACDLEDYTCNSNYLDDLSRHRELVL 509
           LLEACDLEDYT NS YL DL+RHREL L
Sbjct: 481 LLEACDLEDYTINSTYLKDLTRHRELAL 494

[Close]

BLAST of Tan0019322 vs. NCBI nr
Match: XP_022929500.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 845.9 bits (2184), Expect = 1.8e-241
Identity = 422/508 (83.07%), Postives = 454/508 (89.37%), Query Frame = 0


Query: 1   MTAKVETKDQTDQIPETEMQTALGEPGKGLVKEMEETKGEGEMKTKGDEEYPSEEISPRG 60
           MT  VE K+Q ++  E EM+  +      L KEMEE  GEG MK KG E YP +E SPRG
Sbjct: 1   MTVPVEIKNQLEKFLEMEMEMEM-----ELGKEMEEMGGEGGMKIKGVERYPCDENSPRG 60

Query: 61  VFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGDGGSPTAAEKSLLPAVLLQEAGGIPW 120
           VFE+P+ GTDSD SE+           G GGDG  GGSPTAAEK+L+PA LLQE GGIPW
Sbjct: 61  VFEIPIMGTDSDQSES-----------GGGGDGEAGGSPTAAEKALIPAALLQEIGGIPW 120

Query: 121 KTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWRN 180
           +TMIGSIKKK++WR STFPLLAA Y++SRKNFKRKLARIRSAD+ I+SDDIPFCKPSWRN
Sbjct: 121 RTMIGSIKKKTIWRFSTFPLLAAGYELSRKNFKRKLARIRSADEGITSDDIPFCKPSWRN 180

Query: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLSE 240
           YGFSELATATNNFSPENLLGKGGQAEVYKGC +DGQVVAVKRLMKKEKENEERTADFLSE
Sbjct: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCFADGQVVAVKRLMKKEKENEERTADFLSE 240

Query: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAESLEWKIRFKVAVGVAE 300
           LGIIAHINHPNAARLLGFGIDGGLYLVLE++PYGSFAS+LFGAESL WKIRFKVAVGVAE
Sbjct: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEFVPYGSFASSLFGAESLPWKIRFKVAVGVAE 300

Query: 301 GLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360
           GLNYLHRECPRRIIHRDIKASNILL ENY+PLISDFGLAKWLPENWAHHVVFPIEGTFGY
Sbjct: 301 GLNYLHRECPRRIIHRDIKASNILLDENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360

Query: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDP 420
           LAPEYFMHGIVDEKTDVFAYGVLLLELITGR AVDSSRQSLVIWAKPLLD++SFKELTDP
Sbjct: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRHAVDSSRQSLVIWAKPLLDSSSFKELTDP 420

Query: 421 KLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRSL 480
           KLGDDYDQTEM+RTMLAASMCINH+S +RPHMNRVVQLLKGEDGP+DQLKQKS++GDRSL
Sbjct: 421 KLGDDYDQTEMKRTMLAASMCINHTSSLRPHMNRVVQLLKGEDGPVDQLKQKSLNGDRSL 480

Query: 481 LLEACDLEDYTCNSNYLDDLSRHRELVL 509
           LLEACDLEDYT NS YL DL+RHREL L
Sbjct: 481 LLEACDLEDYTINSTYLSDLTRHRELAL 492

[Close]

BLAST of Tan0019322 vs. NCBI nr
Match: XP_022984864.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 842.8 bits (2176), Expect = 1.5e-240
Identity = 423/508 (83.27%), Postives = 452/508 (88.98%), Query Frame = 0


Query: 1   MTAKVETKDQTDQIPETEMQTALGEPGKGLVKEMEETKGEGEMKTKGDEEYPSEEISPRG 60
           MT  VE K+Q ++  E EM+    E    L KEMEE  GEG MK KG E YP  E SPRG
Sbjct: 1   MTVPVEIKNQLEKFLEMEMEI---EMEMELGKEMEEMGGEGGMKIKGLEGYPCVENSPRG 60

Query: 61  VFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGDGGSPTAAEKSLLPAVLLQEAGGIPW 120
           VFE+P+ GTDSD SE+S+G  GDGD          GGSPTA EK+L+PA LLQE GGIPW
Sbjct: 61  VFEIPIMGTDSDQSESSSGGSGDGDA---------GGSPTAVEKALIPAALLQEIGGIPW 120

Query: 121 KTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWRN 180
           KTMIGSIKKK++WR STFPLLAA Y++SRKNFKRKLARIRSAD+ I+SDDIPFCKPSWRN
Sbjct: 121 KTMIGSIKKKTIWRFSTFPLLAAGYELSRKNFKRKLARIRSADEGITSDDIPFCKPSWRN 180

Query: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLSE 240
           YGFSELA ATNNFSPENLLGKGGQAEVYKGC  DGQVVAVKRLMKKEKENEERTADFLSE
Sbjct: 181 YGFSELAIATNNFSPENLLGKGGQAEVYKGCFIDGQVVAVKRLMKKEKENEERTADFLSE 240

Query: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAESLEWKIRFKVAVGVAE 300
           LGIIAHINHPNAARLLGFGIDGGLYLVLE++PYGSFAS+LFGAESL WKIRFKVAVGVAE
Sbjct: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEFVPYGSFASSLFGAESLPWKIRFKVAVGVAE 300

Query: 301 GLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360
           GLNYLHRECPRRIIHRDIKASNILL ENY+PLISDFGLAKWLPENWAHHVVFPIEGTFGY
Sbjct: 301 GLNYLHRECPRRIIHRDIKASNILLDENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360

Query: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDP 420
           LAPEYFMHGIVDEKTDVFAYGVLLLELITGR AVDSSRQSLVIWAKPLLD++SFKELTDP
Sbjct: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRHAVDSSRQSLVIWAKPLLDSSSFKELTDP 420

Query: 421 KLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRSL 480
           KLGDDYDQTEM+RTMLAASMCINH+S +RPHMNRVVQLLKGEDGP+DQLKQKS++GDRSL
Sbjct: 421 KLGDDYDQTEMKRTMLAASMCINHTSSLRPHMNRVVQLLKGEDGPVDQLKQKSLNGDRSL 480

Query: 481 LLEACDLEDYTCNSNYLDDLSRHRELVL 509
           LLEACDLEDYT NS YL DL+RHREL L
Sbjct: 481 LLEACDLEDYTINSTYLSDLTRHRELAL 496

[Close]

BLAST of Tan0019322 vs. ExPASy TrEMBL
Match: A0A5A7UZG2 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G002440 PE=4 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 2.3e-242
Identity = 429/510 (84.12%), Postives = 460/510 (90.20%), Query Frame = 0


Query: 1   MTAKVETKDQTDQIPETEMQTALGEPGKGLVKEMEET-KGEGEMKTKGDEEYPSEEISPR 60
           MT +VE KDQTDQIP+ EM+  +GE GK   KEMEET +GE  MK KG+EE+  +E SPR
Sbjct: 1   MTVQVEIKDQTDQIPQPEME--IGEIGK-RKKEMEETEEGEEGMKVKGEEEFVCDENSPR 60

Query: 61  GVFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGDGGSPTAAEKSLLPAVLLQEAGGIP 120
           GVFE+P+ GTDSD SE+S+G  GD +           G+ +  EK+LLPA LLQE GGIP
Sbjct: 61  GVFEIPIVGTDSDQSESSSGGGGDVE-----------GTLSVPEKALLPAALLQEVGGIP 120

Query: 121 WKTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWR 180
           WK MIG IKKKSVWR ST PLLAASY+ISRKNFKRKLARIRSAD+ I+SDDIPFCKPSWR
Sbjct: 121 WKAMIGCIKKKSVWRFSTIPLLAASYEISRKNFKRKLARIRSADEGITSDDIPFCKPSWR 180

Query: 181 NYGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLS 240
           NYGFSEL+ ATNNFSPENLLGKGGQAEVYKGCLSDGQ+VAVKRLMKKEKENEERTADFLS
Sbjct: 181 NYGFSELSAATNNFSPENLLGKGGQAEVYKGCLSDGQIVAVKRLMKKEKENEERTADFLS 240

Query: 241 ELGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAESLEWKIRFKVAVGVA 300
           ELGIIAHINHPNAARLLGFGI+ GLYLVLE+IP+GSFASALFGAESLEW IRFKVA+GVA
Sbjct: 241 ELGIIAHINHPNAARLLGFGIESGLYLVLEFIPHGSFASALFGAESLEWNIRFKVALGVA 300

Query: 301 EGLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFG 360
           EGLNYLH ECPRRIIHRDIKASNILLAENY+PLISDFGLAKWLPENWAHHVVFPIEGTFG
Sbjct: 301 EGLNYLHCECPRRIIHRDIKASNILLAENYEPLISDFGLAKWLPENWAHHVVFPIEGTFG 360

Query: 361 YLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTD 420
           YLAPEYFMHGIVDEKTDVFA+GVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTD
Sbjct: 361 YLAPEYFMHGIVDEKTDVFAFGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTD 420

Query: 421 PKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRS 480
           PKLGDDYDQTEMRRTMLAASMCINHSS +RPHMNRVVQLLKGEDGPLDQLK KSMDGDRS
Sbjct: 421 PKLGDDYDQTEMRRTMLAASMCINHSSSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRS 480

Query: 481 LLLEACDLEDYTCNSNYLDDLSRHRELVLE 510
            LLEACDLEDYT N++YL+DLSRHRELVLE
Sbjct: 481 FLLEACDLEDYTNNTSYLNDLSRHRELVLE 496

[Close]

BLAST of Tan0019322 vs. ExPASy TrEMBL
Match: A0A6J1EUK1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436050 PE=4 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 8.8e-242
Identity = 422/508 (83.07%), Postives = 454/508 (89.37%), Query Frame = 0


Query: 1   MTAKVETKDQTDQIPETEMQTALGEPGKGLVKEMEETKGEGEMKTKGDEEYPSEEISPRG 60
           MT  VE K+Q ++  E EM+  +      L KEMEE  GEG MK KG E YP +E SPRG
Sbjct: 1   MTVPVEIKNQLEKFLEMEMEMEM-----ELGKEMEEMGGEGGMKIKGVERYPCDENSPRG 60

Query: 61  VFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGDGGSPTAAEKSLLPAVLLQEAGGIPW 120
           VFE+P+ GTDSD SE+           G GGDG  GGSPTAAEK+L+PA LLQE GGIPW
Sbjct: 61  VFEIPIMGTDSDQSES-----------GGGGDGEAGGSPTAAEKALIPAALLQEIGGIPW 120

Query: 121 KTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWRN 180
           +TMIGSIKKK++WR STFPLLAA Y++SRKNFKRKLARIRSAD+ I+SDDIPFCKPSWRN
Sbjct: 121 RTMIGSIKKKTIWRFSTFPLLAAGYELSRKNFKRKLARIRSADEGITSDDIPFCKPSWRN 180

Query: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLSE 240
           YGFSELATATNNFSPENLLGKGGQAEVYKGC +DGQVVAVKRLMKKEKENEERTADFLSE
Sbjct: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCFADGQVVAVKRLMKKEKENEERTADFLSE 240

Query: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAESLEWKIRFKVAVGVAE 300
           LGIIAHINHPNAARLLGFGIDGGLYLVLE++PYGSFAS+LFGAESL WKIRFKVAVGVAE
Sbjct: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEFVPYGSFASSLFGAESLPWKIRFKVAVGVAE 300

Query: 301 GLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360
           GLNYLHRECPRRIIHRDIKASNILL ENY+PLISDFGLAKWLPENWAHHVVFPIEGTFGY
Sbjct: 301 GLNYLHRECPRRIIHRDIKASNILLDENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360

Query: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDP 420
           LAPEYFMHGIVDEKTDVFAYGVLLLELITGR AVDSSRQSLVIWAKPLLD++SFKELTDP
Sbjct: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRHAVDSSRQSLVIWAKPLLDSSSFKELTDP 420

Query: 421 KLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRSL 480
           KLGDDYDQTEM+RTMLAASMCINH+S +RPHMNRVVQLLKGEDGP+DQLKQKS++GDRSL
Sbjct: 421 KLGDDYDQTEMKRTMLAASMCINHTSSLRPHMNRVVQLLKGEDGPVDQLKQKSLNGDRSL 480

Query: 481 LLEACDLEDYTCNSNYLDDLSRHRELVL 509
           LLEACDLEDYT NS YL DL+RHREL L
Sbjct: 481 LLEACDLEDYTINSTYLSDLTRHRELAL 492

[Close]

BLAST of Tan0019322 vs. ExPASy TrEMBL
Match: A0A6J1JBQ7 (receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483013 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 7.5e-241
Identity = 423/508 (83.27%), Postives = 452/508 (88.98%), Query Frame = 0


Query: 1   MTAKVETKDQTDQIPETEMQTALGEPGKGLVKEMEETKGEGEMKTKGDEEYPSEEISPRG 60
           MT  VE K+Q ++  E EM+    E    L KEMEE  GEG MK KG E YP  E SPRG
Sbjct: 1   MTVPVEIKNQLEKFLEMEMEI---EMEMELGKEMEEMGGEGGMKIKGLEGYPCVENSPRG 60

Query: 61  VFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGDGGSPTAAEKSLLPAVLLQEAGGIPW 120
           VFE+P+ GTDSD SE+S+G  GDGD          GGSPTA EK+L+PA LLQE GGIPW
Sbjct: 61  VFEIPIMGTDSDQSESSSGGSGDGDA---------GGSPTAVEKALIPAALLQEIGGIPW 120

Query: 121 KTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWRN 180
           KTMIGSIKKK++WR STFPLLAA Y++SRKNFKRKLARIRSAD+ I+SDDIPFCKPSWRN
Sbjct: 121 KTMIGSIKKKTIWRFSTFPLLAAGYELSRKNFKRKLARIRSADEGITSDDIPFCKPSWRN 180

Query: 181 YGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLSE 240
           YGFSELA ATNNFSPENLLGKGGQAEVYKGC  DGQVVAVKRLMKKEKENEERTADFLSE
Sbjct: 181 YGFSELAIATNNFSPENLLGKGGQAEVYKGCFIDGQVVAVKRLMKKEKENEERTADFLSE 240

Query: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAESLEWKIRFKVAVGVAE 300
           LGIIAHINHPNAARLLGFGIDGGLYLVLE++PYGSFAS+LFGAESL WKIRFKVAVGVAE
Sbjct: 241 LGIIAHINHPNAARLLGFGIDGGLYLVLEFVPYGSFASSLFGAESLPWKIRFKVAVGVAE 300

Query: 301 GLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360
           GLNYLHRECPRRIIHRDIKASNILL ENY+PLISDFGLAKWLPENWAHHVVFPIEGTFGY
Sbjct: 301 GLNYLHRECPRRIIHRDIKASNILLDENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGY 360

Query: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDP 420
           LAPEYFMHGIVDEKTDVFAYGVLLLELITGR AVDSSRQSLVIWAKPLLD++SFKELTDP
Sbjct: 361 LAPEYFMHGIVDEKTDVFAYGVLLLELITGRHAVDSSRQSLVIWAKPLLDSSSFKELTDP 420

Query: 421 KLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRSL 480
           KLGDDYDQTEM+RTMLAASMCINH+S +RPHMNRVVQLLKGEDGP+DQLKQKS++GDRSL
Sbjct: 421 KLGDDYDQTEMKRTMLAASMCINHTSSLRPHMNRVVQLLKGEDGPVDQLKQKSLNGDRSL 480

Query: 481 LLEACDLEDYTCNSNYLDDLSRHRELVL 509
           LLEACDLEDYT NS YL DL+RHREL L
Sbjct: 481 LLEACDLEDYTINSTYLSDLTRHRELAL 496

[Close]

BLAST of Tan0019322 vs. ExPASy TrEMBL
Match: A0A0A0L2T4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G114500 PE=4 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.2e-240
Identity = 427/510 (83.73%), Postives = 461/510 (90.39%), Query Frame = 0


Query: 1   MTAKVETKDQTDQIPETEMQTALGEPGKGLVKEMEET-KGEGEMKTKGDEEYPSEEISPR 60
           MT +VE +DQTDQI + EM+  +GE GK   KEMEET +GE  MK KG+EE+  +E SPR
Sbjct: 1   MTVQVEIEDQTDQILQPEME--IGEIGK-RKKEMEETEEGEEGMKVKGEEEFVCDENSPR 60

Query: 61  GVFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGDGGSPTAAEKSLLPAVLLQEAGGIP 120
           GVFE+P+ GTDSD SE+S+     GDG G+ G+    G+ T AEK+LLP  LLQE GGIP
Sbjct: 61  GVFEIPIVGTDSDQSESSS-----GDGGGEDGE----GTLTVAEKALLPGALLQEVGGIP 120

Query: 121 WKTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWR 180
           WK MIG IKKKSVWR ST PLLAASY+ISRKNFKRKLARIRSAD+ I+ DDIPFCKPSWR
Sbjct: 121 WKAMIGCIKKKSVWRFSTIPLLAASYEISRKNFKRKLARIRSADEGITGDDIPFCKPSWR 180

Query: 181 NYGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLS 240
           NYGF+EL+ ATNNFSPENLLGKGGQAEVYKGCLSDGQ+VAVKRLMKKEKENEERTADFLS
Sbjct: 181 NYGFAELSAATNNFSPENLLGKGGQAEVYKGCLSDGQIVAVKRLMKKEKENEERTADFLS 240

Query: 241 ELGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAESLEWKIRFKVAVGVA 300
           ELGIIAHINHPNAARLLGFGI+ GLYLVLE+IPYGSFASALFGAE LEW IRFKVA+GVA
Sbjct: 241 ELGIIAHINHPNAARLLGFGIESGLYLVLEFIPYGSFASALFGAEPLEWNIRFKVALGVA 300

Query: 301 EGLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFG 360
           EGLNYLHRECPRRIIHRDIKASNILLAENY+PLISDFGLAKWLPENWAHHVVFPIEGTFG
Sbjct: 301 EGLNYLHRECPRRIIHRDIKASNILLAENYEPLISDFGLAKWLPENWAHHVVFPIEGTFG 360

Query: 361 YLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTD 420
           YLAPEYFMHGIVDE+TDVFA+GVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTD
Sbjct: 361 YLAPEYFMHGIVDERTDVFAFGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTD 420

Query: 421 PKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRS 480
           PKLGD+YDQTEM RTMLAASMCINHSS +RPHMNRVVQLLKGEDGPLDQLK KSMDGDRS
Sbjct: 421 PKLGDNYDQTEMGRTMLAASMCINHSSSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRS 480

Query: 481 LLLEACDLEDYTCNSNYLDDLSRHRELVLE 510
           LLLEACDLEDYT NS+YL+DL+RHRELVLE
Sbjct: 481 LLLEACDLEDYTNNSSYLNDLNRHRELVLE 498

[Close]

BLAST of Tan0019322 vs. ExPASy TrEMBL
Match: A0A6J1ENB3 (receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436050 PE=4 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.4e-239
Identity = 412/477 (86.37%), Postives = 439/477 (92.03%), Query Frame = 0


Query: 32  KEMEETKGEGEMKTKGDEEYPSEEISPRGVFEVPVTGTDSDHSETSTGAVGDGDGDGDGG 91
           KEMEE  GEG MK KG E YP +E SPRGVFE+P+ GTDSD SE+           G GG
Sbjct: 6   KEMEEMGGEGGMKIKGVERYPCDENSPRGVFEIPIMGTDSDQSES-----------GGGG 65

Query: 92  DGGDGGSPTAAEKSLLPAVLLQEAGGIPWKTMIGSIKKKSVWRLSTFPLLAASYDISRKN 151
           DG  GGSPTAAEK+L+PA LLQE GGIPW+TMIGSIKKK++WR STFPLLAA Y++SRKN
Sbjct: 66  DGEAGGSPTAAEKALIPAALLQEIGGIPWRTMIGSIKKKTIWRFSTFPLLAAGYELSRKN 125

Query: 152 FKRKLARIRSADDAISSDDIPFCKPSWRNYGFSELATATNNFSPENLLGKGGQAEVYKGC 211
           FKRKLARIRSAD+ I+SDDIPFCKPSWRNYGFSELATATNNFSPENLLGKGGQAEVYKGC
Sbjct: 126 FKRKLARIRSADEGITSDDIPFCKPSWRNYGFSELATATNNFSPENLLGKGGQAEVYKGC 185

Query: 212 LSDGQVVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEYI 271
            +DGQVVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLE++
Sbjct: 186 FADGQVVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEFV 245

Query: 272 PYGSFASALFGAESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENYDP 331
           PYGSFAS+LFGAESL WKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILL ENY+P
Sbjct: 246 PYGSFASSLFGAESLPWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLDENYEP 305

Query: 332 LISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGR 391
           LISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGR
Sbjct: 306 LISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGR 365

Query: 392 RAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSPIRPH 451
            AVDSSRQSLVIWAKPLLD++SFKELTDPKLGDDYDQTEM+RTMLAASMCINH+S +RPH
Sbjct: 366 HAVDSSRQSLVIWAKPLLDSSSFKELTDPKLGDDYDQTEMKRTMLAASMCINHTSSLRPH 425

Query: 452 MNRVVQLLKGEDGPLDQLKQKSMDGDRSLLLEACDLEDYTCNSNYLDDLSRHRELVL 509
           MNRVVQLLKGEDGP+DQLKQKS++GDRSLLLEACDLEDYT NS YL DL+RHREL L
Sbjct: 426 MNRVVQLLKGEDGPVDQLKQKSLNGDRSLLLEACDLEDYTINSTYLSDLTRHRELAL 471

[Close]

BLAST of Tan0019322 vs. TAIR 10
Match: AT5G10520.1 (ROP binding protein kinases 1 )

HSP 1 Score: 520.0 bits (1338), Expect = 2.1e-147
Identity = 268/476 (56.30%), Postives = 350/476 (73.53%), Query Frame = 0


Query: 36  ETKGEGEMKTKGDEEYPSEEISPRGVFEVPVTGTDSDHSETSTGAVGDGDGDGDGGDGGD 95
           E+K   E++   ++    +  SPRGV  +    +DSD+S +S  +              D
Sbjct: 10  ESKNHQEVELHRNDLGLEDSSSPRGVLGMV---SDSDNSSSSCSSC----------SSDD 69

Query: 96  GGSPTAAEKSLLPAVLLQEAGGIPWKTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRK 155
             S T++  S     +     G+ W  MI SIKKKS+ R S  PLL ASY+++RKN +RK
Sbjct: 70  KSSSTSSPFSNTTKTVSSSHHGLQWNKMIESIKKKSMRRFSVIPLL-ASYELTRKNLRRK 129

Query: 156 LARIRSADDAISSDDIPFCKPSWRNYGFSELATATNNFSPENLLGKGGQAEVYKGCLSDG 215
             ++  ++ A + +     KPSWRN+ + ELA AT+ F+PEN++GKGG AEVYKG L +G
Sbjct: 130 QPKLTPSESAFTCEAFFMAKPSWRNFTYEELAVATDYFNPENMIGKGGHAEVYKGVLING 189

Query: 216 QVVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGS 275
           + VA+K+LM   KE EER +DFLSELGIIAH+NHPNAARL GF  D GL+ VLEY PYGS
Sbjct: 190 ETVAIKKLMSHAKEEEERVSDFLSELGIIAHVNHPNAARLRGFSSDRGLHFVLEYAPYGS 249

Query: 276 FASALFGAES-LEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENYDPLIS 335
            AS LFG+E  LEWKIR+KVA+G+A+GL+YLH  CPRRIIHRDIKASNILL  +Y+  IS
Sbjct: 250 LASMLFGSEECLEWKIRYKVALGIADGLSYLHNACPRRIIHRDIKASNILLNHDYEAQIS 309

Query: 336 DFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAV 395
           DFGLAKWLPENW HHVVFPIEGTFGYLAPEYFMHGIVDEK DVFA+GVLLLE+IT RRAV
Sbjct: 310 DFGLAKWLPENWPHHVVFPIEGTFGYLAPEYFMHGIVDEKIDVFAFGVLLLEIITSRRAV 369

Query: 396 D-SSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMN 455
           D +SRQS+V WAKP L+ NS +++ DP+LG+ ++ TEM+R ML ASMC++H + +RP M 
Sbjct: 370 DTASRQSIVAWAKPFLEKNSMEDIVDPRLGNMFNPTEMQRVMLTASMCVHHIAAMRPDMT 429

Query: 456 RVVQLLKGEDGPLDQLKQKSMDGDRSLLLEACDLEDYTCNSNYLDDLSRHRELVLE 510
           R+VQLL+GEDGP  +L+QK+  G+R++ + ACDL+D+T +S+YL++L RHR+L++E
Sbjct: 430 RLVQLLRGEDGPA-ELQQKA--GERTMSVNACDLQDHT-SSSYLNELRRHRQLLME 467

[Close]

BLAST of Tan0019322 vs. TAIR 10
Match: AT5G65530.1 (Protein kinase superfamily protein )

HSP 1 Score: 511.9 bits (1317), Expect = 5.8e-145
Identity = 272/482 (56.43%), Postives = 350/482 (72.61%), Query Frame = 0


Query: 31  VKEMEETKGEGEMKTK-GDEEYPSEEISPRGVFEVPVTGTDSDHSETSTGAVGDGDGDGD 90
           V+EME+ K +G  +T+    +   E+ SPR V E+PV  +DSD+S + +    D      
Sbjct: 3   VEEMEK-KIKGSQETELHKNDLSIEDPSPRCVLEIPVMSSDSDNSSSCSSCSPD------ 62

Query: 91  GGDGGDGGSPTAAEKSLLPAVLLQEAGGIPWKTMIGSIKKKSVWRLSTFPLLAASYDISR 150
                          S L       +    W  MI SIKKKS+ R S  PLL ASY+++R
Sbjct: 63  ------------KSSSPLSTTPPNVSSFHQWNKMIESIKKKSIRRFSVIPLL-ASYELTR 122

Query: 151 KNFKRKLARIRSADDAISSDDIPFCKPSWRNYGFSELATATNNFSPENLLGKGGQAEVYK 210
           K  + KL+     D     D I   KPSWRN+ F EL  AT+NF+PEN++GKGG AEVYK
Sbjct: 123 KKKQPKLSPCSEND--FDCDQILVAKPSWRNFTFDELVAATDNFNPENMIGKGGHAEVYK 182

Query: 211 GCLSDGQVVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLE 270
           G L DG+ VA+K+L +  KE EER +DFLSELGIIAH+NHPNAARL GF  D GL+ VLE
Sbjct: 183 GVLPDGETVAIKKLTRHAKEVEERVSDFLSELGIIAHVNHPNAARLRGFSCDRGLHFVLE 242

Query: 271 YIPYGSFASALFGAES-LEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAEN 330
           Y  +GS AS LFG+E  L+WK R+KVA+G+A+GL+YLH +CPRRIIHRDIKASNILL+++
Sbjct: 243 YSSHGSLASLLFGSEECLDWKKRYKVAMGIADGLSYLHNDCPRRIIHRDIKASNILLSQD 302

Query: 331 YDPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELI 390
           Y+  ISDFGLAKWLPE+W HH+VFPIEGTFGYLAPEYFMHGIVDEKTDVFA+GVLLLE+I
Sbjct: 303 YEAQISDFGLAKWLPEHWPHHIVFPIEGTFGYLAPEYFMHGIVDEKTDVFAFGVLLLEII 362

Query: 391 TGRRAVDS-SRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSP 450
           TGRRAVD+ SRQS+V+WAKPLL+ N+ +E+ DP+LG+D+D+TEM+R M  ASMCI+H S 
Sbjct: 363 TGRRAVDTDSRQSIVMWAKPLLEKNNMEEIVDPQLGNDFDETEMKRVMQTASMCIHHVST 422

Query: 451 IRPHMNRVVQLLKGEDGPLDQLKQKSMDGDRSLLLEACDLEDYTCNSNYLDDLSRHRELV 510
           +RP MNR+VQLL+G+    DQL ++   G R++ L+ CDL D+T +S+YL+DL+RHR+L+
Sbjct: 423 MRPDMNRLVQLLRGD----DQLAEQKPGGARTVSLDDCDL-DHT-SSSYLNDLTRHRQLL 456

[Close]

BLAST of Tan0019322 vs. TAIR 10
Match: AT5G18910.1 (Protein kinase superfamily protein )

HSP 1 Score: 410.2 bits (1053), Expect = 2.4e-114
Identity = 202/383 (52.74%), Postives = 275/383 (71.80%), Query Frame = 0


Query: 128 KKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWRNYGFSELA 187
           K  S    +TF  L     ++R+  KR    +     A+ +D + + KPSWRN+   ++ 
Sbjct: 127 KGSSAMPFNTFTPLKGVPKLTRRKSKRIRDNMVPVIPALDTDHLFYFKPSWRNFSLRDIQ 186

Query: 188 TATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFLSELGIIAHI 247
           TATN++S ENL+G+GG AEVYKG ++DGQ+VA+K+L +     EE T D+LSELGII H+
Sbjct: 187 TATNDYSRENLIGEGGYAEVYKGQMADGQIVAIKKLTRGSA--EEMTMDYLSELGIIVHV 246

Query: 248 NHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGA-ESLEWKIRFKVAVGVAEGLNYLH 307
           +HPN A+L+G+ ++GG++LVLE  P GS AS L+ A E L W +R+KVA+G AEGL YLH
Sbjct: 247 DHPNIAKLIGYCVEGGMHLVLELSPNGSLASLLYEAKEKLNWSMRYKVAMGTAEGLYYLH 306

Query: 308 RECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYF 367
             C RRIIH+DIKASNILL +N++  ISDFGLAKWLP+ W HH V  +EGTFGYL PE+F
Sbjct: 307 EGCQRRIIHKDIKASNILLTQNFEAQISDFGLAKWLPDQWTHHTVSKVEGTFGYLPPEFF 366

Query: 368 MHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDDY 427
           MHGIVDEKTDV+AYGVLLLELITGR+A+DSS+ S+V+WAKPL+  N  K+L DP L DDY
Sbjct: 367 MHGIVDEKTDVYAYGVLLLELITGRQALDSSQHSIVMWAKPLIKENKIKQLVDPILEDDY 426

Query: 428 DQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKGEDGPLDQLKQKSMDG-DRSLLLEAC 487
           D  E+ R +  AS+CI+ +S  RP M++VV++L+G+   LD+L+++      R+   E  
Sbjct: 427 DVEELDRLVFIASLCIHQTSMNRPQMSQVVEILRGDKCSLDKLRERENSKLQRTYSEELL 486

Query: 488 DLEDYTCNSNYLDDLSRHRELVL 509
           D E+Y  ++ YL+D++RH E VL
Sbjct: 487 DNEEYN-STRYLNDINRHMETVL 506

[Close]

BLAST of Tan0019322 vs. TAIR 10
Match: AT5G35960.1 (Protein kinase family protein )

HSP 1 Score: 379.4 bits (973), Expect = 4.5e-105
Identity = 190/344 (55.23%), Postives = 252/344 (73.26%), Query Frame = 0


Query: 120 WKTMIGSIKKKSVWRLSTFPLLAASYDISRKNFKRKLARIRSADDAISSDDIPFCKPSWR 179
           ++ M+  ++ KS   L    +   +  + + +F+R  +   +     SS+D  F   S R
Sbjct: 62  FRKMVQLLRSKSKKSLENVKIPFHNNGVIKSSFRRCSSMRENL--RFSSNDSHFLLHSPR 121

Query: 180 N-YGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTADFL 239
             + FS+L +ATNNFS ENL+GKGG AEVYKG L +GQ+VA+KRLM+    +EE   DFL
Sbjct: 122 RIFTFSDLKSATNNFSLENLIGKGGYAEVYKGMLPNGQMVAIKRLMR--GNSEEIIVDFL 181

Query: 240 SELGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGA-ESLEWKIRFKVAVG 299
           SE+GI+AH+NHPN A+LLG+G++GG++LVLE  P+GS AS L+ + E ++W IR+K+A+G
Sbjct: 182 SEMGIMAHVNHPNIAKLLGYGVEGGMHLVLELSPHGSLASMLYSSKEKMKWSIRYKIALG 241

Query: 300 VAEGLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFPIEGT 359
           VAEGL YLHR C RRIIHRDIKA+NILL  ++ P I DFGLAKWLPENW HH+V   EGT
Sbjct: 242 VAEGLVYLHRGCHRRIIHRDIKAANILLTHDFSPQICDFGLAKWLPENWTHHIVSKFEGT 301

Query: 360 FGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKEL 419
           FGYLAPEY  HGIVDEKTDVFA GVLLLEL+TGRRA+D S+QSLV+WAKPL+  N  +EL
Sbjct: 302 FGYLAPEYLTHGIVDEKTDVFALGVLLLELVTGRRALDYSKQSLVLWAKPLMKKNKIREL 361

Query: 420 TDPKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKG 462
            DP L  +Y+  +++  +LAA++ I  SS  RP M++VV++LKG
Sbjct: 362 IDPSLAGEYEWRQIKLVLLAAALSIQQSSIERPEMSQVVEILKG 401

[Close]

BLAST of Tan0019322 vs. TAIR 10
Match: AT3G05140.1 (ROP binding protein kinases 2 )

HSP 1 Score: 370.5 bits (950), Expect = 2.1e-102
Identity = 201/396 (50.76%), Postives = 265/396 (66.92%), Query Frame = 0


Query: 120 WKTMIGSIKKKSVWRLSTF-PLLAASYDISRKNFKRKLARIRSADDAISS---DDIPFCK 179
           W       K++S+ RLS+F PL  A+  I ++N        +SAD  ++     DI   +
Sbjct: 74  WGKFFKLWKRRSMKRLSSFPPLSGAAPPIIKQN--------KSADPNMNGMVLHDIYDFQ 133

Query: 180 PSWRNYGFSELATATNNFSPENLLGKGGQAEVYKGCLSDGQVVAVKRLMKKEKENEERTA 239
            S +N+  S++  AT+NFSPEN++G+GG A+VY+G L +G+++AVKRL K     +E+TA
Sbjct: 134 SSLQNFSISDIEIATDNFSPENIIGRGGYADVYQGILPEGKLIAVKRLTKGTP--DEQTA 193

Query: 240 DFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEYIPYGSFASALFGAE--SLEWKIRFK 299
           +FLSELGIIAH++HPN A+ +G  I+GG++LV    P GS  S L G     L W  R+ 
Sbjct: 194 EFLSELGIIAHVDHPNTAKFIGCCIEGGMHLVFRLSPLGSLGSLLHGPSKYKLTWSRRYN 253

Query: 300 VAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENYDPLISDFGLAKWLPENWAHHVVFP 359
           VA+G A+GL YLH  C RRIIHRDIKA NILL E++ P I DFGLAKWLP+   HH V  
Sbjct: 254 VALGTADGLVYLHEGCQRRIIHRDIKADNILLTEDFQPQICDFGLAKWLPKQLTHHNVSK 313

Query: 360 IEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNS 419
            EGTFGY APEYFMHGIVDEKTDVFA+GVLLLELITG  A+D S+QSLV+WAKPLL+  +
Sbjct: 314 FEGTFGYFAPEYFMHGIVDEKTDVFAFGVLLLELITGHPALDESQQSLVLWAKPLLERKA 373

Query: 420 FKELTDPKLGDDYDQTEMRRTMLAASMCINHSSPIRPHMNRVVQLLKG-EDGPLDQLKQK 479
            KEL DP LGD+Y++ E+ R    AS+CI+ SS +RP M++VV+LL G ED  +   + K
Sbjct: 374 IKELVDPSLGDEYNREELIRLTSTASLCIDQSSLLRPRMSQVVELLLGHEDVVMTPREAK 433

Query: 480 SMDGDRSLLLEACDLEDYTCNSNYLDDLSRHRELVL 509
                R+   E  D  +Y  ++ YL DL R RE+ L
Sbjct: 434 IKMMQRTYSEELLDSVEYN-STKYLGDLDRIREVAL 458

The following BLAST results are available for this feature:

BLAST of Tan0019322 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H1D6	3.0e-146	56.30	Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thal...	[more]
Q8RXC8	3.0e-101	50.76	Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thal...	[more]
Q5XF57	7.8e-86	51.38	Probable receptor-like serine/threonine-protein kinase At5g57670 OS=Arabidopsis ...	[more]
Q9LV48	5.4e-63	41.30	Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9LK03	9.3e-63	40.88	Proline-rich receptor-like protein kinase PERK2 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Tan0019322 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0060424.1	4.8e-242	84.12	receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucumis melo var. ...	[more]
KAG6577338.1	6.3e-242	83.27	Receptor-like cytosolic serine/threonine-protein kinase RBK1, partial [Cucurbita...	[more]
XP_023552649.1	1.4e-241	83.07	receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 [Cucurbi...	[more]
XP_022929500.1	1.8e-241	83.07	receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 [Cucurbi...	[more]
XP_022984864.1	1.5e-240	83.27	receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 [Cucurbi...	[more]

BLAST of Tan0019322 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UZG2	2.3e-242	84.12	Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo var...	[more]
A0A6J1EUK1	8.8e-242	83.07	receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 OS=Cucur...	[more]
A0A6J1JBQ7	7.5e-241	83.27	receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X1 OS=Cucur...	[more]
A0A0A0L2T4	2.2e-240	83.73	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G114...	[more]
A0A6J1ENB3	2.4e-239	86.37	receptor-like cytosolic serine/threonine-protein kinase RBK1 isoform X2 OS=Cucur...	[more]

BLAST of Tan0019322 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10520.1	2.1e-147	56.30	ROP binding protein kinases 1	[more]
AT5G65530.1	5.8e-145	56.43	Protein kinase superfamily protein	[more]
AT5G18910.1	2.4e-114	52.74	Protein kinase superfamily protein	[more]
AT5G35960.1	4.5e-105	55.23	Protein kinase family protein	[more]
AT3G05140.1	2.1e-102	50.76	ROP binding protein kinases 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 244..264
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 178..293 e-value: 2.0E-24 score: 87.5
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 296..489 e-value: 2.3E-51 score: 176.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 57..79
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 57..125
None	No IPR available	PANTHER	PTHR47987	OS08G0249100 PROTEIN	coord: 63..478
None	No IPR available	PANTHER	PTHR47987:SF7	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 63..478
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 216..482 e-value: 1.8E-19 score: 80.7
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 219..422 e-value: 2.5E-39 score: 135.3
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 216..496 score: 32.951313
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 336..348
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 191..480

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0019322.1	Tan0019322.1	mRNA
Tan0019322.2	Tan0019322.2	mRNA
Tan0019322.3	Tan0019322.3	mRNA
Tan0019322.4	Tan0019322.4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity