Tan0019304 (gene) Snake gourd v1

Overview


Name	Tan0019304
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	LG07: 6248401 .. 6249140 (-)
RNA-Seq Expression	Tan0019304
Synteny	Tan0019304

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAAAGCCAAAACCAACCAAAGTAGTTCCAAGAGTGATATGGAAAGTGGATTTAGCAAACAAACTCTCTTCTTCTTTCTTGTTGTTGTTGCTATTGGTCCCTACAAAAACACAACCTCAGCAACAAGAGACCTTATCCCCAAAGCCACTACCCAGTTCATCAAAACCTCATGCACAGCAACCACCTACCCTAAACTTTGCTTCACATCCCTCTCAGCTCATGCAAACAAGATCCAAACGAACCCGGAGCTGCTGACGAAAACCGCCATCGACGTAGCCCTTTCGTCGACCGAAACGACGTCGAGCACGGTGGAGAAGGTGCTGAAAGGCAAAGGGCAGCTGAGCCCCAGAGAGTTGGGGGCTGTGAAAGATTGTGTGGAGGAGGTGAGAGACTCCATGGATGAGATGAAGAAGTCAGTTAGTGAAATGAAGAAGCTCAAAGGAGGTGTTGATTTCGAGATGACAATGAGCAATATTCAGACGTGGATAAGCGCAGCATTGACAGATGAAGATGCTTGCATGGAGGGCTTTGGTGGGAAGTCCATGGATGGCGATGTGAAGGTTTTTGTGAGACAACATATTCAGAAGATTGCTCATTTGACTAGCAATGCATTGGCTTTGGTTAACAGATTTGCTGATCTTCATGCCTAGTTTTTTTATCTATAAATCATATTTGAGCAAAAGGGTGTTTTTAATTATGTTTAATTAGTCTTAATTATTGTACAATAATGTGTAATCTTA

mRNA sequence

CCAAAGCCAAAACCAACCAAAGTAGTTCCAAGAGTGATATGGAAAGTGGATTTAGCAAACAAACTCTCTTCTTCTTTCTTGTTGTTGTTGCTATTGGTCCCTACAAAAACACAACCTCAGCAACAAGAGACCTTATCCCCAAAGCCACTACCCAGTTCATCAAAACCTCATGCACAGCAACCACCTACCCTAAACTTTGCTTCACATCCCTCTCAGCTCATGCAAACAAGATCCAAACGAACCCGGAGCTGCTGACGAAAACCGCCATCGACGTAGCCCTTTCGTCGACCGAAACGACGTCGAGCACGGTGGAGAAGGTGCTGAAAGGCAAAGGGCAGCTGAGCCCCAGAGAGTTGGGGGCTGTGAAAGATTGTGTGGAGGAGGTGAGAGACTCCATGGATGAGATGAAGAAGTCAGTTAGTGAAATGAAGAAGCTCAAAGGAGGTGTTGATTTCGAGATGACAATGAGCAATATTCAGACGTGGATAAGCGCAGCATTGACAGATGAAGATGCTTGCATGGAGGGCTTTGGTGGGAAGTCCATGGATGGCGATGTGAAGGTTTTTGTGAGACAACATATTCAGAAGATTGCTCATTTGACTAGCAATGCATTGGCTTTGGTTAACAGATTTGCTGATCTTCATGCCTAGTTTTTTTATCTATAAATCATATTTGAGCAAAAGGGTGTTTTTAATTATGTTTAATTAGTCTTAATTATTGTACAATAATGTGTAATCTTA

Coding sequence (CDS)

ATGGAAAGTGGATTTAGCAAACAAACTCTCTTCTTCTTTCTTGTTGTTGTTGCTATTGGTCCCTACAAAAACACAACCTCAGCAACAAGAGACCTTATCCCCAAAGCCACTACCCAGTTCATCAAAACCTCATGCACAGCAACCACCTACCCTAAACTTTGCTTCACATCCCTCTCAGCTCATGCAAACAAGATCCAAACGAACCCGGAGCTGCTGACGAAAACCGCCATCGACGTAGCCCTTTCGTCGACCGAAACGACGTCGAGCACGGTGGAGAAGGTGCTGAAAGGCAAAGGGCAGCTGAGCCCCAGAGAGTTGGGGGCTGTGAAAGATTGTGTGGAGGAGGTGAGAGACTCCATGGATGAGATGAAGAAGTCAGTTAGTGAAATGAAGAAGCTCAAAGGAGGTGTTGATTTCGAGATGACAATGAGCAATATTCAGACGTGGATAAGCGCAGCATTGACAGATGAAGATGCTTGCATGGAGGGCTTTGGTGGGAAGTCCATGGATGGCGATGTGAAGGTTTTTGTGAGACAACATATTCAGAAGATTGCTCATTTGACTAGCAATGCATTGGCTTTGGTTAACAGATTTGCTGATCTTCATGCCTAG

Protein sequence

MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSAHANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSMDEMKKSVSEMKKLKGGVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQHIQKIAHLTSNALALVNRFADLHA

Homology

[Close]

BLAST of Tan0019304 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.7e-39
Identity = 95/199 (47.74%), Postives = 124/199 (62.31%), Query Frame = 0


Query: 6   SKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSAHANKI 65
           SK TL F  ++V +        A     P    QFIKTSCT TTYP +C  SLSA+A  I
Sbjct: 4   SKSTLVFSALLVIL--------AAASAAP--ANQFIKTSCTLTTYPAVCEQSLSAYAKTI 63

Query: 66  QTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSMDEMKK 125
           Q NP+ L  TA+ V+L+ T+   + ++++ K KG L  R+  A+ DC+EEV DS+D + +
Sbjct: 64  QNNPQELASTALQVSLTRTQQAQTFMKRLNKFKG-LKARQYAAIHDCLEEVEDSLDRVSR 123

Query: 126 SVSEMKKLK--GGVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQHIQK 185
           S  EMK L    G DF   MSN++TW+SAALTDE  CM+GF GK MDG +K  VR  +  
Sbjct: 124 SCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVA 183

Query: 186 IAHLTSNALALVNRFADLH 203
           +A +TSNALALVN FA  H
Sbjct: 184 VARVTSNALALVNNFAAKH 191

[Close]

BLAST of Tan0019304 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.1e-34
Identity = 77/168 (45.83%), Postives = 115/168 (68.45%), Query Frame = 0


Query: 38  TQFIKTSCTATTYPKLCFTSLSAHANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKG 97
           T+FIK SC  T+YP  CF SLS++A++I+  P  L +TA+ V+++  ++  + V ++   
Sbjct: 35  TEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDY 94

Query: 98  KGQLSPRELGAVKDCVEEVRDSMDEMKKSVSEMKKLK---GGVDFEMTMSNIQTWISAAL 157
           KG ++ R+  AV DC+EE+ D++D +  S+ E+K L+    G DF   +SN++TW SAAL
Sbjct: 95  KG-ITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAAL 154

Query: 158 TDEDACMEGFGGKSMDGDVKVFVRQHIQKIAHLTSNALALVNRFADLH 203
           TDE ACM+GFGGK+M G++K  +R HI  +A  TSNALAL+N FA  H
Sbjct: 155 TDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFASKH 201

[Close]

BLAST of Tan0019304 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 7.5e-32
Identity = 88/195 (45.13%), Postives = 118/195 (60.51%), Query Frame = 0


Query: 9   TLFFFLVVVAIGPYKNTTSATRDLI-PKATTQFIKTSCTATTYPKLCFTSLSAHANKIQT 68
           TLF FL+  AI     ++SA R  I  K    FI+ SC ATTYP +C  SL+ +AN IQT
Sbjct: 8   TLFLFLLSTAI--LTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQT 67

Query: 69  NPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSMDEMKKSV 128
           +P  L +TA++V ++  ++T   V + L     L  RE+ AVKDC+EE+ D++D +  S+
Sbjct: 68  SPRRLAETALNVTVTQAQSTKVFVWR-LGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSI 127

Query: 129 SEMK---KLKGGVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQHIQKI 188
            E+K     KG   F   MSN QTW SAALT+ + C +GF G+ MDG VK  VR  I  +
Sbjct: 128 HEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNL 187

Query: 189 AHLTSNALALVNRFA 200
              TSNALAL+N FA
Sbjct: 188 GRGTSNALALINAFA 199

[Close]

BLAST of Tan0019304 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.7e-29
Identity = 78/192 (40.62%), Postives = 120/192 (62.50%), Query Frame = 0


Query: 13  FLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSAHANKIQ-TNPEL 72
           FLV++       ++SAT +     + QFI +SC  T YP LC  +LSA+A KI+  N + 
Sbjct: 8   FLVILLSITILQSSSATPN--RSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQD 67

Query: 73  LTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSMDEMKKSVSEMK 132
           L +TA+ ++L+  ++ +  V K+ K   +   RE  A+KDC+E + +S+D + +SV E+ 
Sbjct: 68  LAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELA 127

Query: 133 KLKGGV---DFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQHIQKIAHLT 192
           +    V   DF   MSN+QTW+SAALTDE  C++GF  ++M G VK  +R  +  +A +T
Sbjct: 128 RAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVT 187

Query: 193 SNALALVNRFAD 201
           SNALALVN+FA+
Sbjct: 188 SNALALVNQFAE 197

[Close]

BLAST of Tan0019304 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.7e-29
Identity = 71/162 (43.83%), Postives = 109/162 (67.28%), Query Frame = 0


Query: 40  FIKTSCTATTYPKLCFTSLSAHANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKG 99
           +IKTSC  T Y  +C+ SLS +A+ I++NP+ L   A+++ LSS ++ S  V+ +  G G
Sbjct: 147 YIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHG-G 206

Query: 100 QLSPRELGAVKDCVEEVRDSMDEMKKSVSEMKKL--KGGVDFEMTMSNIQTWISAALTDE 159
            L+  E+ AV DCVEE+ DS+  ++ S+ E+  +  K    FEM MS+++TW+SAALT++
Sbjct: 207 GLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTND 266

Query: 160 DACMEGFGGKSMDGDVKVFVRQHIQKIAHLTSNALALVNRFA 200
           D CM+GF    +   VK  VR+H+ ++A LTSNALAL+N +A
Sbjct: 267 DTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of Tan0019304 vs. NCBI nr
Match: KAG6605706.1 (hypothetical protein SDJN03_03023, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 323.9 bits (829), Expect = 9.6e-85
Identity = 168/204 (82.35%), Postives = 184/204 (90.20%), Query Frame = 0


Query: 1   MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSA 60
           MESG+SKQ L FF+++ AI P KN+T+AT DL+PKATTQFIK SCTATTYPKLCFTSLSA
Sbjct: 1   MESGYSKQILIFFVLLAAIDPNKNSTTATTDLVPKATTQFIKASCTATTYPKLCFTSLSA 60

Query: 61  HANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSM 120
           HANKIQTNPELLTKTAIDVAL STETTSSTV  +LK KGQLS RE+GAV DCVEE+RDSM
Sbjct: 61  HANKIQTNPELLTKTAIDVALLSTETTSSTVANLLKSKGQLSRREVGAVTDCVEELRDSM 120

Query: 121 DEMKKSVSEMKKLKG--GVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVR 180
           D +K+SVSEMKKLKG  G DFEMTMSNIQTWISAALTDEDACMEGFGG+SMDG VK FV+
Sbjct: 121 DRLKRSVSEMKKLKGGDGFDFEMTMSNIQTWISAALTDEDACMEGFGGRSMDGYVKAFVK 180

Query: 181 QHIQKIAHLTSNALALVNRFADLH 203
           QHI+ IAHLTSNALALVN+FADLH
Sbjct: 181 QHIEMIAHLTSNALALVNKFADLH 204

[Close]

BLAST of Tan0019304 vs. NCBI nr
Match: XP_023533934.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo] >XP_023533935.1 21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 323.9 bits (829), Expect = 9.6e-85
Identity = 169/204 (82.84%), Postives = 184/204 (90.20%), Query Frame = 0


Query: 1   MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSA 60
           MESGFSKQ L FF+++ AI P KN+T+AT DLIPKATTQFIK SCTATTYPKLCFTSLSA
Sbjct: 1   MESGFSKQILIFFVLLAAIDPNKNSTTATTDLIPKATTQFIKASCTATTYPKLCFTSLSA 60

Query: 61  HANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSM 120
           HANKI+TNPELLTKTAIDVAL STETTSSTV  +LK KGQLS RE+GAV DCVEE+RDSM
Sbjct: 61  HANKIRTNPELLTKTAIDVALLSTETTSSTVANLLKSKGQLSRREVGAVTDCVEELRDSM 120

Query: 121 DEMKKSVSEMKKLKG--GVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVR 180
           D +K+SVSEMKKLKG  G DFEMTMSNIQTWISAALTDEDACMEGFGG+SMDG VK FV+
Sbjct: 121 DRLKRSVSEMKKLKGGDGFDFEMTMSNIQTWISAALTDEDACMEGFGGRSMDGYVKAFVK 180

Query: 181 QHIQKIAHLTSNALALVNRFADLH 203
           QHI+ IAHLTSNALALVN+FADLH
Sbjct: 181 QHIEMIAHLTSNALALVNKFADLH 204

[Close]

BLAST of Tan0019304 vs. NCBI nr
Match: XP_022958522.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 322.8 bits (826), Expect = 2.1e-84
Identity = 168/204 (82.35%), Postives = 183/204 (89.71%), Query Frame = 0


Query: 1   MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSA 60
           MESG+SKQ   FF+++ AI P KN+T+AT DLIPKATTQFIK SCTATTYPKLCFTSLSA
Sbjct: 1   MESGYSKQIFIFFVLLAAIDPNKNSTTATTDLIPKATTQFIKASCTATTYPKLCFTSLSA 60

Query: 61  HANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSM 120
           HANKIQTNPELLTKTAIDVAL STETTSSTV  +LK KGQLS RE+GAV DCVEE+RDSM
Sbjct: 61  HANKIQTNPELLTKTAIDVALLSTETTSSTVANLLKSKGQLSRREVGAVTDCVEELRDSM 120

Query: 121 DEMKKSVSEMKKLKG--GVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVR 180
           D +K+SVSEMKKLKG  G DFEMTMSNIQTWISAALTDEDACMEGFGG+SMDG VK FV+
Sbjct: 121 DRLKRSVSEMKKLKGGDGFDFEMTMSNIQTWISAALTDEDACMEGFGGRSMDGYVKAFVK 180

Query: 181 QHIQKIAHLTSNALALVNRFADLH 203
           QHI+ IAHLTSNALALVN+FADLH
Sbjct: 181 QHIEMIAHLTSNALALVNKFADLH 204

[Close]

BLAST of Tan0019304 vs. NCBI nr
Match: XP_022996077.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 317.8 bits (813), Expect = 6.9e-83
Identity = 166/204 (81.37%), Postives = 181/204 (88.73%), Query Frame = 0


Query: 1   MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSA 60
           MESG+SKQ L FF+++ AI P KN+T+AT DLIPKAT QFIK SCTATTYPKLCFTSLSA
Sbjct: 1   MESGYSKQILIFFVLLAAIDPNKNSTTATTDLIPKATAQFIKASCTATTYPKLCFTSLSA 60

Query: 61  HANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSM 120
           HANKIQTNPELL KTAIDVAL STETTSSTV  +LK KGQLS RE+GAV DCVEE+RDSM
Sbjct: 61  HANKIQTNPELLMKTAIDVALLSTETTSSTVANLLKSKGQLSRREVGAVTDCVEELRDSM 120

Query: 121 DEMKKSVSEMKKLKG--GVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVR 180
           D +K+SVSEMKKLKG  G DFEMTMSNIQTWISAALTDEDAC EGFGG+SMDG VK FV+
Sbjct: 121 DRLKRSVSEMKKLKGGDGFDFEMTMSNIQTWISAALTDEDACTEGFGGRSMDGYVKAFVK 180

Query: 181 QHIQKIAHLTSNALALVNRFADLH 203
           QHI+ IAHLTSNALALVN+FADLH
Sbjct: 181 QHIEMIAHLTSNALALVNKFADLH 204

[Close]

BLAST of Tan0019304 vs. NCBI nr
Match: EOY22919.1 (DC1.2-like, putative [Theobroma cacao])

HSP 1 Score: 204.1 bits (518), Expect = 1.1e-48
Identity = 104/202 (51.49%), Postives = 151/202 (74.75%), Query Frame = 0


Query: 1   MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSA 60
           ME  F   +  F L+++AI  Y N +SA R + P+++ +FI+TSC++TTYPKLCF +LS 
Sbjct: 1   MEGTFCSHSFKFLLILLAINSYINFSSAGRQIAPQSSAEFIRTSCSSTTYPKLCFETLST 60

Query: 61  HANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSM 120
           HA+ IQT+P+LL   A++V LS+TE+TS+ +  + K  G L PRE  A++DCVEE+ DS+
Sbjct: 61  HASLIQTSPQLLAHAALNVTLSTTESTSAMMVTLSKSHG-LKPREAEAMQDCVEELSDSI 120

Query: 121 DEMKKSVSEMKKLKGGVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQH 180
           DE++KS+SEM ++KG  +F + ++++QTW+SAALTDE  C +GF G +M+G+VK  VR  
Sbjct: 121 DELRKSISEMGQVKGS-NFGLMINDVQTWVSAALTDESTCSDGFQGNNMNGNVKTAVRTK 180

Query: 181 IQKIAHLTSNALALVNRFADLH 203
           I +IAHLTSNALAL+N +A LH
Sbjct: 181 ILRIAHLTSNALALINNYASLH 200

[Close]

BLAST of Tan0019304 vs. ExPASy TrEMBL
Match: A0A6J1H224 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111459728 PE=4 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.0e-84
Identity = 168/204 (82.35%), Postives = 183/204 (89.71%), Query Frame = 0


Query: 1   MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSA 60
           MESG+SKQ   FF+++ AI P KN+T+AT DLIPKATTQFIK SCTATTYPKLCFTSLSA
Sbjct: 1   MESGYSKQIFIFFVLLAAIDPNKNSTTATTDLIPKATTQFIKASCTATTYPKLCFTSLSA 60

Query: 61  HANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSM 120
           HANKIQTNPELLTKTAIDVAL STETTSSTV  +LK KGQLS RE+GAV DCVEE+RDSM
Sbjct: 61  HANKIQTNPELLTKTAIDVALLSTETTSSTVANLLKSKGQLSRREVGAVTDCVEELRDSM 120

Query: 121 DEMKKSVSEMKKLKG--GVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVR 180
           D +K+SVSEMKKLKG  G DFEMTMSNIQTWISAALTDEDACMEGFGG+SMDG VK FV+
Sbjct: 121 DRLKRSVSEMKKLKGGDGFDFEMTMSNIQTWISAALTDEDACMEGFGGRSMDGYVKAFVK 180

Query: 181 QHIQKIAHLTSNALALVNRFADLH 203
           QHI+ IAHLTSNALALVN+FADLH
Sbjct: 181 QHIEMIAHLTSNALALVNKFADLH 204

[Close]

BLAST of Tan0019304 vs. ExPASy TrEMBL
Match: A0A6J1K0X4 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111491395 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.3e-83
Identity = 166/204 (81.37%), Postives = 181/204 (88.73%), Query Frame = 0


Query: 1   MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSA 60
           MESG+SKQ L FF+++ AI P KN+T+AT DLIPKAT QFIK SCTATTYPKLCFTSLSA
Sbjct: 1   MESGYSKQILIFFVLLAAIDPNKNSTTATTDLIPKATAQFIKASCTATTYPKLCFTSLSA 60

Query: 61  HANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSM 120
           HANKIQTNPELL KTAIDVAL STETTSSTV  +LK KGQLS RE+GAV DCVEE+RDSM
Sbjct: 61  HANKIQTNPELLMKTAIDVALLSTETTSSTVANLLKSKGQLSRREVGAVTDCVEELRDSM 120

Query: 121 DEMKKSVSEMKKLKG--GVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVR 180
           D +K+SVSEMKKLKG  G DFEMTMSNIQTWISAALTDEDAC EGFGG+SMDG VK FV+
Sbjct: 121 DRLKRSVSEMKKLKGGDGFDFEMTMSNIQTWISAALTDEDACTEGFGGRSMDGYVKAFVK 180

Query: 181 QHIQKIAHLTSNALALVNRFADLH 203
           QHI+ IAHLTSNALALVN+FADLH
Sbjct: 181 QHIEMIAHLTSNALALVNKFADLH 204

[Close]

BLAST of Tan0019304 vs. ExPASy TrEMBL
Match: A0A061G793 (DC1.2-like, putative OS=Theobroma cacao OX=3641 GN=TCM_014946 PE=4 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 5.4e-49
Identity = 104/202 (51.49%), Postives = 151/202 (74.75%), Query Frame = 0


Query: 1   MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSA 60
           ME  F   +  F L+++AI  Y N +SA R + P+++ +FI+TSC++TTYPKLCF +LS 
Sbjct: 1   MEGTFCSHSFKFLLILLAINSYINFSSAGRQIAPQSSAEFIRTSCSSTTYPKLCFETLST 60

Query: 61  HANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSM 120
           HA+ IQT+P+LL   A++V LS+TE+TS+ +  + K  G L PRE  A++DCVEE+ DS+
Sbjct: 61  HASLIQTSPQLLAHAALNVTLSTTESTSAMMVTLSKSHG-LKPREAEAMQDCVEELSDSI 120

Query: 121 DEMKKSVSEMKKLKGGVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQH 180
           DE++KS+SEM ++KG  +F + ++++QTW+SAALTDE  C +GF G +M+G+VK  VR  
Sbjct: 121 DELRKSISEMGQVKGS-NFGLMINDVQTWVSAALTDESTCSDGFQGNNMNGNVKTAVRTK 180

Query: 181 IQKIAHLTSNALALVNRFADLH 203
           I +IAHLTSNALAL+N +A LH
Sbjct: 181 ILRIAHLTSNALALINNYASLH 200

[Close]

BLAST of Tan0019304 vs. ExPASy TrEMBL
Match: A0A7J8LJ63 (PMEI domain-containing protein OS=Gossypium lobatum OX=34289 GN=Golob_023299 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 9.2e-49
Identity = 105/202 (51.98%), Postives = 149/202 (73.76%), Query Frame = 0


Query: 1   MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSA 60
           M S FS  +  F L++VA     N +SA R + PK + +FI+TSC++TTYPKLC+ SL  
Sbjct: 1   MASAFSYDSFGFLLILVAFSSLINISSAGRQIAPKTSIEFIRTSCSSTTYPKLCYESLLT 60

Query: 61  HANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSM 120
            A+ IQT+P+L+   A++V LS+ ++TS+ + K+ K +G L P E+GA+KDCVEE+ D++
Sbjct: 61  QASMIQTSPQLIAHAALNVTLSTAKSTSALMLKLAKSQG-LKPTEVGAMKDCVEELEDTI 120

Query: 121 DEMKKSVSEMKKLKGGVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQH 180
           DE++KS+SEM +LKG  +F + +++IQTW+SAALTDE+ C +GFGGK+M+G VK  VR  
Sbjct: 121 DELRKSISEMGQLKGS-NFGLMINDIQTWVSAALTDENTCADGFGGKNMNGSVKTAVRSK 180

Query: 181 IQKIAHLTSNALALVNRFADLH 203
           I  IAHLTSNALALVN +A  H
Sbjct: 181 IVTIAHLTSNALALVNSYASFH 200

[Close]

BLAST of Tan0019304 vs. ExPASy TrEMBL
Match: A0A6P5YC43 (21 kDa protein OS=Durio zibethinus OX=66656 GN=LOC111290822 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 9.2e-49
Identity = 106/202 (52.48%), Postives = 150/202 (74.26%), Query Frame = 0


Query: 1   MESGFSKQTLFFFLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSA 60
           M S  S  +  F L+++A+  Y N +SA R + P+  T+FI+TSCT+T+YPKLCF SLS 
Sbjct: 1   MASLCSDHSFKFLLILLALNSYINFSSAGRQIAPQTNTEFIRTSCTSTSYPKLCFESLST 60

Query: 61  HANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSM 120
           HA+ IQT+P+LL   A++V LSS ++TS+ +  + K  G L PRE+ A++DCVEE+ D++
Sbjct: 61  HASLIQTSPQLLAHAALNVTLSSAKSTSAMMVTLSKSHG-LQPREVEAMQDCVEELSDTI 120

Query: 121 DEMKKSVSEMKKLKGGVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQH 180
           DE++KS+SEM ++ G  +F + +++IQTW+SAALTDE  C +GFGGK+M+G+VK  VR  
Sbjct: 121 DELRKSISEMDQINGS-NFGLMINDIQTWVSAALTDESTCSDGFGGKNMNGNVKTAVRTK 180

Query: 181 IQKIAHLTSNALALVNRFADLH 203
           I  IAHLTSNALALVN +A LH
Sbjct: 181 IVTIAHLTSNALALVNNYASLH 200

[Close]

BLAST of Tan0019304 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 177.9 bits (450), Expect = 8.0e-45
Identity = 91/190 (47.89%), Postives = 139/190 (73.16%), Query Frame = 0


Query: 13  FLVVVAIGPYKNTTSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSAHANKIQTNPELL 72
           FL+++A+    +TT+ T        T+F+K+SCT TTYP+LCF+SLS HA+ IQT+P+L+
Sbjct: 17  FLIIIAMLKLVHTTTTT-TTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLM 76

Query: 73  TKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSMDEMKKSVSEMKK 132
              A+++ L+S + TS+ + ++     +L P+E+ A++DCVEE+ D+++E++KS+ EM +
Sbjct: 77  AHAALNITLASAKVTSAMMVRL--SNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQ 136

Query: 133 LKGGVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQHIQKIAHLTSNAL 192
           L G  ++E+ +S+IQTW+SAALTD + C +GF G+ MDG VKV VR  I  IAHLTSNAL
Sbjct: 137 LSGS-NYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNAL 196

Query: 193 ALVNRFADLH 203
           AL+N FA +H
Sbjct: 197 ALINHFASIH 202

[Close]

BLAST of Tan0019304 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.1e-37
Identity = 92/199 (46.23%), Postives = 124/199 (62.31%), Query Frame = 0


Query: 6   SKQTLFFFLVVVAIGPYKNT--TSATRDLIPKATTQFIKTSCTATTYPKLCFTSLSAHAN 65
           +KQ LF  L +  +   + T  T+A++    K    FI++SC  TTYP LC  SLS +AN
Sbjct: 2   AKQYLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYAN 61

Query: 66  KIQTNPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSMDEM 125
            IQT+P+ L +TAI V LS  ++T   V ++ + KG L  RE+ A+KDCVEE+ D++D +
Sbjct: 62  DIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKG-LKKREVEAIKDCVEEMNDTVDRL 121

Query: 126 KKSVSEMKKLKGGVD---FEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQH 185
            KSV E+K      D   F   MSN QTW SAALTDE+ C +GF G+ MDG +K  VR  
Sbjct: 122 TKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRAR 181

Query: 186 IQKIAHLTSNALALVNRFA 200
           I  + H TSNAL+L+N FA
Sbjct: 182 IMNVGHETSNALSLINAFA 199

[Close]

BLAST of Tan0019304 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 1.5e-35
Identity = 77/168 (45.83%), Postives = 115/168 (68.45%), Query Frame = 0


Query: 38  TQFIKTSCTATTYPKLCFTSLSAHANKIQTNPELLTKTAIDVALSSTETTSSTVEKVLKG 97
           T+FIK SC  T+YP  CF SLS++A++I+  P  L +TA+ V+++  ++  + V ++   
Sbjct: 35  TEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDY 94

Query: 98  KGQLSPRELGAVKDCVEEVRDSMDEMKKSVSEMKKLK---GGVDFEMTMSNIQTWISAAL 157
           KG ++ R+  AV DC+EE+ D++D +  S+ E+K L+    G DF   +SN++TW SAAL
Sbjct: 95  KG-ITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAAL 154

Query: 158 TDEDACMEGFGGKSMDGDVKVFVRQHIQKIAHLTSNALALVNRFADLH 203
           TDE ACM+GFGGK+M G++K  +R HI  +A  TSNALAL+N FA  H
Sbjct: 155 TDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFASKH 201

[Close]

BLAST of Tan0019304 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 138.7 bits (348), Expect = 5.3e-33
Identity = 88/195 (45.13%), Postives = 118/195 (60.51%), Query Frame = 0


Query: 9   TLFFFLVVVAIGPYKNTTSATRDLI-PKATTQFIKTSCTATTYPKLCFTSLSAHANKIQT 68
           TLF FL+  AI     ++SA R  I  K    FI+ SC ATTYP +C  SL+ +AN IQT
Sbjct: 8   TLFLFLLSTAI--LTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQT 67

Query: 69  NPELLTKTAIDVALSSTETTSSTVEKVLKGKGQLSPRELGAVKDCVEEVRDSMDEMKKSV 128
           +P  L +TA++V ++  ++T   V + L     L  RE+ AVKDC+EE+ D++D +  S+
Sbjct: 68  SPRRLAETALNVTVTQAQSTKVFVWR-LGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSI 127

Query: 129 SEMK---KLKGGVDFEMTMSNIQTWISAALTDEDACMEGFGGKSMDGDVKVFVRQHIQKI 188
            E+K     KG   F   MSN QTW SAALT+ + C +GF G+ MDG VK  VR  I  +
Sbjct: 128 HEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNL 187

Query: 189 AHLTSNALALVNRFA 200
              TSNALAL+N FA
Sbjct: 188 GRGTSNALALINAFA 199

[Close]

BLAST of Tan0019304 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 131.7 bits (330), Expect = 6.5e-31
Identity = 76/173 (43.93%), Postives = 112/173 (64.74%), Query Frame = 0


Query: 34  PKATTQFIKTSCTATTYPKLCFTSLSAHANKIQTNPELLTKTAIDVALSSTETTSSTVEK 93
           P ++  FI +SC  T Y  LC   L+A A+KI+ N   L +TA+ V L   ++T+  V K
Sbjct: 33  PSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTALAVTLVRVQSTTIYVGK 92

Query: 94  VLKGKGQLSPRELGAVKDCVEEVRDSMDEMKKSVSEMKKL-KGGVD---FEMTMSNIQTW 153
           + K + ++  RE  AVKDCVE + D ++ + +S+ E+K++ + G D   F   +SN++TW
Sbjct: 93  LTKAR-RIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSGRDRDEFLWRLSNVETW 152

Query: 154 ISAALTDEDACMEGFGGKSMDGDVKVFVRQHIQKIAHLTSNALALVNRFADLH 203
           +SAALTDE  C++GF GK MDG VK  +R+ +  +A +TSNALALVNRFA  H
Sbjct: 153 VSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNALALVNRFAARH 204

The following BLAST results are available for this feature:

BLAST of Tan0019304 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	3.7e-39	47.74	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB37	2.1e-34	45.83	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9STY5	7.5e-32	45.13	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SI72	2.7e-29	40.63	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SI74	2.7e-29	43.83	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]

BLAST of Tan0019304 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6605706.1	9.6e-85	82.35	hypothetical protein SDJN03_03023, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023533934.1	9.6e-85	82.84	21 kDa protein-like [Cucurbita pepo subsp. pepo] >XP_023533935.1 21 kDa protein-...	[more]
XP_022958522.1	2.1e-84	82.35	21 kDa protein-like [Cucurbita moschata]	[more]
XP_022996077.1	6.9e-83	81.37	21 kDa protein-like [Cucurbita maxima]	[more]
EOY22919.1	1.1e-48	51.49	DC1.2-like, putative [Theobroma cacao]	[more]

BLAST of Tan0019304 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H224	1.0e-84	82.35	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111459728 PE=4 SV=1	[more]
A0A6J1K0X4	3.3e-83	81.37	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111491395 PE=4 SV=1	[more]
A0A061G793	5.4e-49	51.49	DC1.2-like, putative OS=Theobroma cacao OX=3641 GN=TCM_014946 PE=4 SV=1	[more]
A0A7J8LJ63	9.2e-49	51.98	PMEI domain-containing protein OS=Gossypium lobatum OX=34289 GN=Golob_023299 PE=...	[more]
A0A6P5YC43	9.2e-49	52.48	21 kDa protein OS=Durio zibethinus OX=66656 GN=LOC111290822 PE=4 SV=1	[more]

BLAST of Tan0019304 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	8.0e-45	47.89	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	2.1e-37	46.23	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	1.5e-35	45.83	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	5.3e-33	45.13	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	6.5e-31	43.93	pectin methylesterase inhibitor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 113..133
None	No IPR available	PANTHER	PTHR31080:SF96	21 KDA PROTEIN-LIKE	coord: 13..200
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 13..200
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 43..199 e-value: 4.52199E-44 score: 142.577
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 36..194 e-value: 5.2E-47 score: 172.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..196 e-value: 3.3E-35 score: 119.0
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 40..194 e-value: 4.5E-35 score: 121.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 35..198 e-value: 6.0E-40 score: 138.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 39..198

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0019304.1	Tan0019304.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0004857	enzyme inhibitor activity