Tan0019099 (gene) Snake gourd v1

Overview
NameTan0019099
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionchitotriosidase-1-like
LocationLG08: 2832335 .. 2833903 (+)
RNA-Seq ExpressionTan0019099
SyntenyTan0019099
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCAAGCCATCATGTGCATGCTGGTTACTGGTTCACCAACACAAATTTCCAGCTTCCAGCTGCAAATATTGATGCTAATTATTTCACGCATCTCTTTTGTGGTCATGCTGGTATCGACGCTCAAACTTATGAAGTCATCATCGATCCTATGGAAGATGACTTAATTTATGAATTTACTCCGACCGTTAGACTAAGCAATCATAAGGTGAAAACCCTATTATCCATTGGAGGAGAGAAAGATGTGTTTGCTAAAATGGCTAGCGAGCCTACAAATCGTGATGCCTTTATCGAATCCTCCATTTCAATGGCAAGAGCGGGTGAATTTGATGGTCTCGATCTTCAATGGTTTTTTCCATCTTCTCAAGATGAAATGGATGAGTTTGAAAATGTCCTTTCTGAATGGCATAAAGCTGTTATCAAAGAAGCTAAAGATTACCATAGAACAAAATTGGTGCTTGTTGCCACAGTCTCCAACATACCTTATCTACAACACAATATTCAATATCCAATTGATACAATCATACAGACTTTGGATTGGGTTAATTTGATTTCTTATGATTTTTACACCCCTACTTCTTCATCCAACTTTACTGGACCATCCTCAGCCTTCTGCAACCCAAGGGCCAACTCTTTGTCTGTCAATTCCGGTATGAATTAATTAAAAGTTAAATATATGTTTTAGTCCTTAATGTTTCTTTTAAATTAATCGTTTAAAATTGATCGATGATAAATTTACATTAGTCTGTCGGTTCTTGACGAGTTGACAAAATTTTAGAGAAAGAGTTAAGGTAGAAAAGAAGTTTCTAATTCTGAAATTTTAAAATTTCTTCTCTTAAATGATTTAATTTCTTCTATCATAGTATGTAAGAGTATATCATTAGCTAATTTATTCCTAAAACGAAAAGAGGTATAATTAAAAGAGGCATAATTTTAGCTATACAATAAACATAAGAATATCATTTAAATTGTTTTTTTTTTTAAAACAAAAAGGGCTCTAGTATATATATGTTGAAAAAACACTCAAAACAGTAAAACCCAAAACATAAATTTAGCCTCAATTAAATAACACCACACTGCACTTTCCTTTTATTTCACTTTGCATTTATACATATATGCTTAATATTGATTATTGGGTTGAATTGATTGGGCAGGAATTGAGGCATGGATTAAATGCTATCCTCATTTGCCTTCTAGAAAAATTGTATTTGGAATTCCTTTTCATGGTTGGGCATGGAAACTTGCCAACGGTCATCAACATCAAGTATTTTCGAAAGCAAATGGACCAGCCATAGGAGACAATATTTCTTCTGATGGCCGAATCGACTACTACAATATTATAAAGTTTATAGAGGATAATCAAGCGGAAAATGTTAGAAAGGACGAGAGCTATGTAATTGGGTACACATTTTATGATTCAACATGGATTGCCTACGAAAATGAAGCCACCATACCAGAGAAGATTAGACTTGCAAAGTCACACCATGTGCATGGTTATTTTGCTTGGAACATTGCAGCCGATGATGATGTTAACACTCTTGCTAATGCAGCTCAAAGTAGTTGGTGA

mRNA sequence

ATGTCCTCAAGCCATCATGTGCATGCTGGTTACTGGTTCACCAACACAAATTTCCAGCTTCCAGCTGCAAATATTGATGCTAATTATTTCACGCATCTCTTTTGTGGTCATGCTGGTATCGACGCTCAAACTTATGAAGTCATCATCGATCCTATGGAAGATGACTTAATTTATGAATTTACTCCGACCGTTAGACTAAGCAATCATAAGGTGAAAACCCTATTATCCATTGGAGGAGAGAAAGATGTGTTTGCTAAAATGGCTAGCGAGCCTACAAATCGTGATGCCTTTATCGAATCCTCCATTTCAATGGCAAGAGCGGGTGAATTTGATGGTCTCGATCTTCAATGGTTTTTTCCATCTTCTCAAGATGAAATGGATGAGTTTGAAAATGTCCTTTCTGAATGGCATAAAGCTGTTATCAAAGAAGCTAAAGATTACCATAGAACAAAATTGGTGCTTGTTGCCACAGTCTCCAACATACCTTATCTACAACACAATATTCAATATCCAATTGATACAATCATACAGACTTTGGATTGGGTTAATTTGATTTCTTATGATTTTTACACCCCTACTTCTTCATCCAACTTTACTGGACCATCCTCAGCCTTCTGCAACCCAAGGGCCAACTCTTTGTCTGTCAATTCCGGAATTGAGGCATGGATTAAATGCTATCCTCATTTGCCTTCTAGAAAAATTGTATTTGGAATTCCTTTTCATGGTTGGGCATGGAAACTTGCCAACGGTCATCAACATCAAGTATTTTCGAAAGCAAATGGACCAGCCATAGGAGACAATATTTCTTCTGATGGCCGAATCGACTACTACAATATTATAAAGTTTATAGAGGATAATCAAGCGGAAAATGTTAGAAAGGACGAGAGCTATGTAATTGGGTACACATTTTATGATTCAACATGGATTGCCTACGAAAATGAAGCCACCATACCAGAGAAGATTAGACTTGCAAAGTCACACCATGTGCATGGTTATTTTGCTTGGAACATTGCAGCCGATGATGATGTTAACACTCTTGCTAATGCAGCTCAAAGTAGTTGGTGA

Coding sequence (CDS)

ATGTCCTCAAGCCATCATGTGCATGCTGGTTACTGGTTCACCAACACAAATTTCCAGCTTCCAGCTGCAAATATTGATGCTAATTATTTCACGCATCTCTTTTGTGGTCATGCTGGTATCGACGCTCAAACTTATGAAGTCATCATCGATCCTATGGAAGATGACTTAATTTATGAATTTACTCCGACCGTTAGACTAAGCAATCATAAGGTGAAAACCCTATTATCCATTGGAGGAGAGAAAGATGTGTTTGCTAAAATGGCTAGCGAGCCTACAAATCGTGATGCCTTTATCGAATCCTCCATTTCAATGGCAAGAGCGGGTGAATTTGATGGTCTCGATCTTCAATGGTTTTTTCCATCTTCTCAAGATGAAATGGATGAGTTTGAAAATGTCCTTTCTGAATGGCATAAAGCTGTTATCAAAGAAGCTAAAGATTACCATAGAACAAAATTGGTGCTTGTTGCCACAGTCTCCAACATACCTTATCTACAACACAATATTCAATATCCAATTGATACAATCATACAGACTTTGGATTGGGTTAATTTGATTTCTTATGATTTTTACACCCCTACTTCTTCATCCAACTTTACTGGACCATCCTCAGCCTTCTGCAACCCAAGGGCCAACTCTTTGTCTGTCAATTCCGGAATTGAGGCATGGATTAAATGCTATCCTCATTTGCCTTCTAGAAAAATTGTATTTGGAATTCCTTTTCATGGTTGGGCATGGAAACTTGCCAACGGTCATCAACATCAAGTATTTTCGAAAGCAAATGGACCAGCCATAGGAGACAATATTTCTTCTGATGGCCGAATCGACTACTACAATATTATAAAGTTTATAGAGGATAATCAAGCGGAAAATGTTAGAAAGGACGAGAGCTATGTAATTGGGTACACATTTTATGATTCAACATGGATTGCCTACGAAAATGAAGCCACCATACCAGAGAAGATTAGACTTGCAAAGTCACACCATGTGCATGGTTATTTTGCTTGGAACATTGCAGCCGATGATGATGTTAACACTCTTGCTAATGCAGCTCAAAGTAGTTGGTGA

Protein sequence

MSSSHHVHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRLSNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLISYDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW
Homology
BLAST of Tan0019099 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.7e-68
Identity = 148/354 (41.81%), Postives = 208/354 (58.76%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V A YWF  + F  P  +ID++ FTHLFC  A +++QT +V +          FT TV+ 
Sbjct: 28  VKASYWFPASEF--PVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 67  SNHKVKTLLSIGG---EKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQ 126
            N  VKTLLSIGG   +K  +A MAS PT+R +FI+SSI +AR+  F GLDL W +PSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 127 DEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATV--SNIPYLQHNIQYPIDTIIQTLDW 186
            EM  F  +L EW  AV+ EA    + +L+L A V  SN  Y   ++ YP+  +  +LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 187 VNLISYDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFH 246
           VNL++YDFY P   S  TGP +A  +P     S ++G  +WI+    LP++K V G P++
Sbjct: 208 VNLMAYDFYGP-GWSRVTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPYY 267

Query: 247 GWAWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIG- 306
           G+AW+L N + H  ++    P  G  IS DG I Y  I KFI DN A  V    S V+G 
Sbjct: 268 GYAWRLTNANSHSYYA----PTTGAAISPDGSIGYGQIRKFIVDNGATTVY--NSTVVGD 327

Query: 307 YTFYDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           Y +  + WI Y++  +I  K+R AK   + GYF+W++ ADD+ + L+ AA  +W
Sbjct: 328 YCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

BLAST of Tan0019099 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 7.2e-46
Identity = 122/365 (33.42%), Postives = 198/365 (54.25%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPT--- 66
           V A YW   ++F    ++ID NYFTH+           Y   + P  +    E T +   
Sbjct: 55  VRAAYWPAWSDFS--TSSIDTNYFTHI-----------YYAFVQPAPESFNLEITESYKK 114

Query: 67  -------VRLSNHKVKTLLSIGG---EKDVFAKMASEPTNRDAFIESSISMARAGEFDGL 126
                  +     +V TLLSIGG      +F++MAS   NR +FI S+I +AR  EF+GL
Sbjct: 115 WAPKYDGIHNIRPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGL 174

Query: 127 DLQWFFPSSQDEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATV---SNIPYLQHNIQ- 186
           DL W +P  + +M     +L EW+KA++ EA    +++L+L + V   S I  + +  + 
Sbjct: 175 DLDWEWPGDEKDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRS 234

Query: 187 YPIDTIIQTLDWVNLISYDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHL 246
           YP+  I + LDW + + +D+    + +N TG ++A  +P +N +S   GI +WI     +
Sbjct: 235 YPVRAIRKYLDWASPMCFDY--NGAWANETGFNAALYDPNSN-ISTKYGIGSWIG--SGV 294

Query: 247 PSRKIVFGIPFHGWAWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAE 306
           P+ K+V G+P +G AW+L + + H V +KA GPA    + +DG +DY  I+ F +D  A+
Sbjct: 295 PAEKLVMGLPLYGRAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDEILVFNKDTGAK 354

Query: 307 NVRKDESYVIGYTFYDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANA 355
            V  DE  V  Y++  +TWI Y++  +I +K++ A+S  + GYF W I  D D  T++  
Sbjct: 355 -VVYDEVAVSFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDW-TISKQ 395

BLAST of Tan0019099 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.4e-44
Identity = 121/365 (33.15%), Postives = 189/365 (51.78%), Query Frame = 0

Query: 2   SSSHHVHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDD------ 61
           SS+  V   YW  N  F  P ++ID + FTH+F      +  TY+  ++  E+D      
Sbjct: 32  SSTTRVKGIYWIENPLF--PPSSIDTSLFTHIFYAFVSPNKFTYK--LEEEEEDSTTVAT 91

Query: 62  LIYEFTPTVRLSNHKVKTLLSIGG---EKDVFAKMASEPTNRDAFIESSISMARAGEFDG 121
            +  FT T +     + TLLSIGG      +FA +AS+PT R  FI S+I +AR   FDG
Sbjct: 92  SLTTFTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDG 151

Query: 122 LDLQWFFPSSQDEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATV---SNIPYLQHNIQ 181
           +D  W FP++  EM++   +L +W +A+  E     R  L+L A V    N         
Sbjct: 152 IDFDWEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRM 211

Query: 182 YPIDTIIQTLDWVNLISYDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHL 241
           YP+D+I + LDWVN++SYD     S SN TG  S   + ++N +SV SG+ +WI+    +
Sbjct: 212 YPVDSINKNLDWVNVMSYDL--RGSGSNVTGAPSGMFDSKSN-VSVVSGLFSWIR--GGV 271

Query: 242 PSRKIVFGIPFHGWAWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAE 301
              KIV G+P +G +WKL + + H +      P +G     DG + Y+ ++ F +   A+
Sbjct: 272 APEKIVMGMPLYGKSWKLQDPNVHGI----GAPNVGPGPGVDGGMAYFQVVDFNKQMGAK 331

Query: 302 NVRKDESYVIGYTFYDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANA 355
            V   E+  + Y++  STWI Y++  T+  K+  A++  + GYF W  A  D  +   + 
Sbjct: 332 VVYDKETGSV-YSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYFFW-AAGYDTSDWKVST 381

BLAST of Tan0019099 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.1e-43
Identity = 116/359 (32.31%), Postives = 179/359 (49.86%), Query Frame = 0

Query: 2   SSSHHVHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFT 61
           SS   V   YW  N  F  P   +D + FTH+F      +  TY++ I   +   +  FT
Sbjct: 30  SSITRVKGIYWLENPFF--PPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFT 89

Query: 62  PTVRLSNHKVKTLLSIGG---EKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWF 121
            T +  +    TL SIGG      + A +AS+P    AFI S+I +AR   FDG+DL W 
Sbjct: 90  KTFKTKSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWE 149

Query: 122 FPSSQDEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATV---SNIPYLQHNIQYPIDTI 181
           FP +  EM++   +L +W KA+  E     R  L+L A V    N         YP+++I
Sbjct: 150 FPKNTKEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSI 209

Query: 182 IQTLDWVNLISYDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIV 241
            + LDWVN++SY+   P   SN TG  S   +P++N +SV SG+ +WI  +  +   K+V
Sbjct: 210 NENLDWVNVMSYELRGP--RSNKTGAPSGTFDPKSN-VSVVSGLLSWI--HSGVVPEKLV 269

Query: 242 FGIPFHGWAWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDE 301
            G+P +G +WKL + + H +      P++G     +G + Y+ ++ F     A+ V  D 
Sbjct: 270 MGMPLYGKSWKLRDPNVHGI----GAPSVGSGPGVNGLMAYFQVLDFNRQKSAK-VEYDV 329

Query: 302 SYVIGYTFYDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
                Y++  STWI Y+N  T+  K+  A++  + GYF W    D     +A  A  +W
Sbjct: 330 DTASVYSYSGSTWIGYDNPFTVSIKVGFAQALKLRGYFFWVAGLDTLDWKIATQASKAW 376

BLAST of Tan0019099 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 9.1e-41
Identity = 113/360 (31.39%), Postives = 193/360 (53.61%), Query Frame = 0

Query: 4   SHHVHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPT 63
           +H V + YW    +F    + ID NYFTH+       +  ++++ I          F   
Sbjct: 37  NHGVRSAYWPAGDDFS--PSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKA 96

Query: 64  VRLSNHKVKTLLSIGG---EKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFP 123
           +R  +  VKTLLSIGG      +F+++AS   NR+ FI S+I +AR   FDG+DL W FP
Sbjct: 97  LRHRSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFP 156

Query: 124 SSQDEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATV---SNIPYL-QHNIQ-YPIDTI 183
            +Q +M     +  EW+ A+  EAK   + +L+L + V   S +  + +H  + YP   I
Sbjct: 157 ETQQDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAI 216

Query: 184 IQTLDWVNLISYDFY-TPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKI 243
            + LDW + + +D++ T  ++++F    +A      + +S N G+ +WIK    +   K+
Sbjct: 217 NKYLDWASPMCFDYHGTWDNNTDF----NAALYDSKSEISTNFGLHSWIK--SGVRPEKL 276

Query: 244 VFGIPFHGWAWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKD 303
           V G+  +G AW+L + + + V ++A GPA      +DG ++Y  I+KF + + A NV  D
Sbjct: 277 VMGLALYGRAWELKDPNVNGVGAEAVGPA----TDTDGSMNYNEILKFNKQSGA-NVVYD 336

Query: 304 ESYVIGYTFYDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           +  +  Y++  +TWI Y++  +I  K+R AKS  + GYF W +  D D  +++  A ++W
Sbjct: 337 KVAISFYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKDW-SISKQASNAW 382

BLAST of Tan0019099 vs. NCBI nr
Match: XP_022143191.1 (chitotriosidase-1-like [Momordica charantia])

HSP 1 Score: 504.6 bits (1298), Expect = 6.9e-139
Identity = 244/349 (69.91%), Postives = 290/349 (83.09%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V A YWF +TN +LP+ANIDAN+FTHLFCG+A ++ QTYEV ID  E  +I  FT TV L
Sbjct: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSL 65

Query: 67  SNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEM 126
           SN  VKTLLSI G+  V AKMASEP NR+ FI+SSI  A+ G FDGLDLQW +PSSQ+EM
Sbjct: 66  SNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQEEM 125

Query: 127 DEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLIS 186
           + FE++L EWH AV+++A++YHR +L+LVA VSN+P + HNIQYPIDTIIQTLDWVNL S
Sbjct: 126 ENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNLFS 185

Query: 187 YDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWK 246
           YDFYTPTSS   TGPSSA  NP+ +SLSVNSGIEAWIKCYP+LPS++IVFGIPFHGWAWK
Sbjct: 186 YDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWAWK 245

Query: 247 LANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYDS 306
           LA+  QH VFS+A+G AIG +ISSDG+I Y NI KFIE N+A+N++ D  YVIGYT++D+
Sbjct: 246 LADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTYFDT 305

Query: 307 TWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQ-SSW 355
           TWIAYENE TIPEK+RLAKS HV GYFA NIAADDDVNTLANAA+ +SW
Sbjct: 306 TWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVNTLANAAKANSW 354

BLAST of Tan0019099 vs. NCBI nr
Match: KAG7030350.1 (Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 438.7 bits (1127), Expect = 4.7e-119
Identity = 211/348 (60.63%), Postives = 268/348 (77.01%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V+AGYW  +T+ +LPAANIDA  FTHLF G+A +++ TY+V IDP    LI +F+ TV+L
Sbjct: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSSTVKL 70

Query: 67  SNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEM 126
           SN +VKTLLSI  +  +FA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQD+M
Sbjct: 71  SNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 127 DEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLIS 186
           D F+ ++  WH+AV+ +A      +L+LVATVSN+PYLQ NIQYP D I Q LDWVNL+S
Sbjct: 131 DNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 190

Query: 187 YDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWK 246
           YDFYTP SS   TGPSSAF NP+AN+LS N GI +W++   +LP +KIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGIPFHGWAWK 250

Query: 247 LANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYDS 306
           L N HQH +F+KA+G A G+ I   G+I Y ++ KFIEDNQA    KD+SY++ Y +Y++
Sbjct: 251 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYET 310

Query: 307 TWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           TWI YE+E TI +KI LAK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 311 TWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Tan0019099 vs. NCBI nr
Match: XP_022946077.1 (acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mammalian chitinase-like [Cucurbita moschata])

HSP 1 Score: 438.0 bits (1125), Expect = 8.0e-119
Identity = 211/348 (60.63%), Postives = 268/348 (77.01%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V+AGYW  +T+ +LPAANIDA  FTHLF G+A +++ TY+V +DP +  LI +F+ TV+L
Sbjct: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSSTVKL 70

Query: 67  SNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEM 126
           SN +VKTLLSI  +  VFA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQD+M
Sbjct: 71  SNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 127 DEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLIS 186
           D F+ ++  WH+AV+ +A      +L+LVATVSN+PYLQ NIQYP D I Q LDWVNL+S
Sbjct: 131 DNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 190

Query: 187 YDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWK 246
           YDFYTP SS   TGPSSAF NP+AN+LS N GI +W +   +LP +KIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 250

Query: 247 LANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYDS 306
           L N HQH +F+KA+G A G+ I   G+I Y ++ KFIEDNQA    KD+SY++ Y +Y++
Sbjct: 251 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYET 310

Query: 307 TWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           TWI YE+E TI +KI LAK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 311 TWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Tan0019099 vs. NCBI nr
Match: KAG6599362.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1 Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 438.0 bits (1125), Expect = 8.0e-119
Identity = 211/348 (60.63%), Postives = 268/348 (77.01%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V+AGYW  +T+ +LPAANIDA  FTHLF G+A +++ TY+V IDP +  LI +F+ TV+L
Sbjct: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILIDQFSSTVKL 70

Query: 67  SNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEM 126
           SN +VKTLLSI  +  +FA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQD+M
Sbjct: 71  SNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 127 DEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLIS 186
           D F+ ++  WH+AV+ +A      +L+LVATVSN+PYLQ NIQYP D I Q LDWVNL+S
Sbjct: 131 DNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 190

Query: 187 YDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWK 246
           YDFYTP SS   TGPSSAF NP+AN+LS N GI +W +   +LP +KIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 250

Query: 247 LANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYDS 306
           L N HQH +F+KA+G A G+ I   G+I Y ++ KFIEDNQA    KD+SY++ Y +Y++
Sbjct: 251 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYET 310

Query: 307 TWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           TWI YE+E TI +KI LAK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 311 TWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Tan0019099 vs. NCBI nr
Match: XP_023545402.1 (acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 437.2 bits (1123), Expect = 1.4e-118
Identity = 210/348 (60.34%), Postives = 268/348 (77.01%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V+AGYW  +T+ +LPAANIDA  FTHLF G+A +++ TY+V IDP +  L+ +F+PTVRL
Sbjct: 2   VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSPTVRL 61

Query: 67  SNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEM 126
           SN  VKTLLSI  +  VFA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQ+EM
Sbjct: 62  SNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQEEM 121

Query: 127 DEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLIS 186
             F+ ++  WH+AV+ +A      +L+LVATVSN+PYLQ NIQYP D I Q LDWVNL+S
Sbjct: 122 GNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 181

Query: 187 YDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWK 246
           YDFYTP S+   TGPSSAF NP+AN+LS N GI +W +   +LP +KIVFGIPFHGWAWK
Sbjct: 182 YDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 241

Query: 247 LANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYDS 306
           L N HQH +F+KA+G A G+ I   G+I Y ++ KFIEDNQA    KD+SY++ YT++++
Sbjct: 242 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYTYFET 301

Query: 307 TWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           TWI YE++ TI +KI LAK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 302 TWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 349

BLAST of Tan0019099 vs. ExPASy TrEMBL
Match: A0A6J1CPI6 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013120 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.4e-139
Identity = 244/349 (69.91%), Postives = 290/349 (83.09%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V A YWF +TN +LP+ANIDAN+FTHLFCG+A ++ QTYEV ID  E  +I  FT TV L
Sbjct: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSL 65

Query: 67  SNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEM 126
           SN  VKTLLSI G+  V AKMASEP NR+ FI+SSI  A+ G FDGLDLQW +PSSQ+EM
Sbjct: 66  SNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQEEM 125

Query: 127 DEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLIS 186
           + FE++L EWH AV+++A++YHR +L+LVA VSN+P + HNIQYPIDTIIQTLDWVNL S
Sbjct: 126 ENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNLFS 185

Query: 187 YDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWK 246
           YDFYTPTSS   TGPSSA  NP+ +SLSVNSGIEAWIKCYP+LPS++IVFGIPFHGWAWK
Sbjct: 186 YDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWAWK 245

Query: 247 LANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYDS 306
           LA+  QH VFS+A+G AIG +ISSDG+I Y NI KFIE N+A+N++ D  YVIGYT++D+
Sbjct: 246 LADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTYFDT 305

Query: 307 TWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQ-SSW 355
           TWIAYENE TIPEK+RLAKS HV GYFA NIAADDDVNTLANAA+ +SW
Sbjct: 306 TWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVNTLANAAKANSW 354

BLAST of Tan0019099 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 3.9e-119
Identity = 211/348 (60.63%), Postives = 268/348 (77.01%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V+AGYW  +T+ +LPAANIDA  FTHLF G+A +++ TY+V +DP +  LI +F+ TV+L
Sbjct: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSSTVKL 70

Query: 67  SNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEM 126
           SN +VKTLLSI  +  VFA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQD+M
Sbjct: 71  SNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 127 DEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLIS 186
           D F+ ++  WH+AV+ +A      +L+LVATVSN+PYLQ NIQYP D I Q LDWVNL+S
Sbjct: 131 DNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 190

Query: 187 YDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWK 246
           YDFYTP SS   TGPSSAF NP+AN+LS N GI +W +   +LP +KIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 250

Query: 247 LANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYDS 306
           L N HQH +F+KA+G A G+ I   G+I Y ++ KFIEDNQA    KD+SY++ Y +Y++
Sbjct: 251 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYET 310

Query: 307 TWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           TWI YE+E TI +KI LAK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 311 TWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Tan0019099 vs. ExPASy TrEMBL
Match: A0A6J1DYG4 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 4.3e-118
Identity = 211/349 (60.46%), Postives = 264/349 (75.64%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V+ GYW ++TN QLP +NIDA  FTHLFCG A ID  +YEV +   +  LI +F+ TVR+
Sbjct: 11  VNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQFSSTVRM 70

Query: 67  SNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEM 126
           SN  VKTLLSI G++DVFAKMASEPT RD FI+SSI +A  G+F+GLDLQW +PSS++EM
Sbjct: 71  SNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSEEEM 130

Query: 127 DEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLIS 186
             FE +L  W +A + +AK YHR + +LVA+VSN+PY++ +I YP++ I Q LDWVNLIS
Sbjct: 131 QNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLDWVNLIS 190

Query: 187 YDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWK 246
           YDFYTP SS + TGPSSA  N  A SLS N GI+AWI+   +LP ++IVFGIPFHGWAWK
Sbjct: 191 YDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPFHGWAWK 250

Query: 247 LANGHQHQVFSKANGPAIGDN-ISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYD 306
           LAN  +H VFSKA GPA GD  IS +G+ID  N+ +FI+ N A+ V+ D  Y I Y +  
Sbjct: 251 LANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRYSISYAYAG 310

Query: 307 STWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           +TWIAYE+E TIP+KI  AKS  V GYFAWNIAADD+VNTLANAAQ++W
Sbjct: 311 TTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNW 359

BLAST of Tan0019099 vs. ExPASy TrEMBL
Match: A0A6J1KFT3 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 1.2e-117
Identity = 208/348 (59.77%), Postives = 266/348 (76.44%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V+AGYW  +T+  LPAANIDA  FTHLF G+A +++ TY+V +DP +  LI +F+ TV+L
Sbjct: 11  VNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSSTVKL 70

Query: 67  SNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEM 126
           SN  VKTLLSI G+  VFA MA +PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQD+M
Sbjct: 71  SNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 127 DEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLIS 186
             F+ ++  WH+AV+ +A      +L+LVATVSN+PYLQ+NIQYP D I Q LDWVNL+S
Sbjct: 131 ANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLDWVNLLS 190

Query: 187 YDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWK 246
           YDFYTP S  + TGPSSAF NP+AN+LS N GI +W +   +L  +KIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGIPFHGWAWK 250

Query: 247 LANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYDS 306
           LAN HQH +F+KA+G A G+ I   G+I Y ++ KFIEDNQA    KD+SY++ Y +Y++
Sbjct: 251 LANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYET 310

Query: 307 TWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           TWI +E+E T+ +KI LAK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 311 TWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Tan0019099 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.6e-117
Identity = 208/348 (59.77%), Postives = 266/348 (76.44%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V+AGYW  +T+  LPAANIDA  FTHLF G+A +++ TY+V +DP +  LI +F+ TV+L
Sbjct: 11  VNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSSTVKL 70

Query: 67  SNHKVKTLLSIGGEKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQDEM 126
           SN  VKTLLSI G+  VFA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQD+M
Sbjct: 71  SNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 127 DEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVNLIS 186
             F+ ++  WH+AV+ +A      +L+LVATVSN+PYLQ+NIQYP D I Q LDWVNL+S
Sbjct: 131 ANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLDWVNLLS 190

Query: 187 YDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGWAWK 246
           YDFYTP S    TGPSSAF NP+AN+LS N GI +W +   +L  +KIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPFHGWAWK 250

Query: 247 LANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTFYDS 306
           LAN HQH +F+KA+G A G+ I   G+I Y ++ KFIEDNQA    KD+SY++ Y +Y++
Sbjct: 251 LANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYET 310

Query: 307 TWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           TWI +E+E T+ +KI LAK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 311 TWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Tan0019099 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 259.2 bits (661), Expect = 4.7e-69
Identity = 148/354 (41.81%), Postives = 208/354 (58.76%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V A YWF  + F  P  +ID++ FTHLFC  A +++QT +V +          FT TV+ 
Sbjct: 28  VKASYWFPASEF--PVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 67  SNHKVKTLLSIGG---EKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQ 126
            N  VKTLLSIGG   +K  +A MAS PT+R +FI+SSI +AR+  F GLDL W +PSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 127 DEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATV--SNIPYLQHNIQYPIDTIIQTLDW 186
            EM  F  +L EW  AV+ EA    + +L+L A V  SN  Y   ++ YP+  +  +LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 187 VNLISYDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFH 246
           VNL++YDFY P   S  TGP +A  +P     S ++G  +WI+    LP++K V G P++
Sbjct: 208 VNLMAYDFYGP-GWSRVTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPYY 267

Query: 247 GWAWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIG- 306
           G+AW+L N + H  ++    P  G  IS DG I Y  I KFI DN A  V    S V+G 
Sbjct: 268 GYAWRLTNANSHSYYA----PTTGAAISPDGSIGYGQIRKFIVDNGATTVY--NSTVVGD 327

Query: 307 YTFYDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSSW 355
           Y +  + WI Y++  +I  K+R AK   + GYF+W++ ADD+ + L+ AA  +W
Sbjct: 328 YCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

BLAST of Tan0019099 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 248.8 bits (634), Expect = 6.4e-66
Identity = 144/348 (41.38%), Postives = 203/348 (58.33%), Query Frame = 0

Query: 1   MSSSHHVHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEF 60
           MS S  V A YWF  T+F  PA +ID++ FTHLFC  A ++A++YE+ I        + F
Sbjct: 1   MSESDVVKASYWFPATDF--PATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAF 60

Query: 61  TPTVRLSNHKVKTLLSIGG---EKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQW 120
           T TV+  N  VKTLLSIGG   +KD FA MAS P +R +FI+S+I++AR+  F GLDL W
Sbjct: 61  TETVQQRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDW 120

Query: 121 FFPSSQDEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQ---YPIDT 180
            +P +++EM +F  +L EW  AV  E+     T L+L A V    Y   N Q   YP+  
Sbjct: 121 EYPRNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLA 180

Query: 181 IIQTLDWVNLISYDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKI 240
           I  +LDW+NL++YDFY P   S  TGP ++   P  +  S +SG+  W +    LP++K 
Sbjct: 181 ISNSLDWINLMAYDFYGP-GWSTVTGPPASLYLP-TDGRSGDSGVRDWTEA--GLPAKKA 240

Query: 241 VFGIPFHGWAWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKD 300
           V G P++GWAW LA+   +   +   GPAI D    DG I Y  +  +I DN A  V  D
Sbjct: 241 VLGFPYYGWAWTLADPDVNGYDANTTGPAISD----DGEISYRQLQTWIVDNGATKVH-D 300

Query: 301 ESYVIGYTFYDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDD 343
           +  V  Y +  +TWI Y++E +I  K+  AK   + GYF+W++  DD+
Sbjct: 301 DMMVGDYCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDN 333

BLAST of Tan0019099 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 247.3 bits (630), Expect = 1.9e-65
Identity = 139/339 (41.00%), Postives = 196/339 (57.82%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V A YWF  +  + P A ID++ FTHLFC  A I+  TY+VI+          FT TVR 
Sbjct: 26  VKATYWFAES--ESPLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVRR 85

Query: 67  SNHKVKTLLSIGGE---KDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQ 126
            N  VKTLLSIGG+      FA MAS PT+R  FI SSI +AR+  F GLDL W +PS  
Sbjct: 86  RNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSIT 145

Query: 127 DEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVN 186
            EMD F  +L EW  AV  EA+   + +L+L A V    Y  +++ +P++ +  +LDWVN
Sbjct: 146 TEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVNAVADSLDWVN 205

Query: 187 LISYDFYTPTSSSNFTGPSSAFCNPRANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHGW 246
           L++YDFY   SS     P+  + +P     S ++G+ AW +    LP++K V G P +G+
Sbjct: 206 LVAYDFYESGSSRVTCSPAPLY-DPITTGPSGDAGVRAWTQA--GLPAKKAVLGFPLYGY 265

Query: 247 AWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYTF 306
           AW L +   H  ++ ++GPA    IS DG I Y  I +FI DN+A  V  + + V  Y +
Sbjct: 266 AWCLTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKATMV-YNSNLVQNYCY 325

Query: 307 YDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDD 343
              TWI Y++  +I  K++ AK   + GYF+W+I ADD+
Sbjct: 326 AKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDN 353

BLAST of Tan0019099 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 229.6 bits (584), Expect = 4.0e-60
Identity = 134/338 (39.64%), Postives = 188/338 (55.62%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQLPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIYEFTPTVRL 66
           V A YW        PA NID+  FTHLFC  A +D+ T+EV I       +  FT TV+ 
Sbjct: 14  VKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTVKD 73

Query: 67  SNHKVKTLLSIGG---EKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDLQWFFPSSQ 126
            N  V+TLLSIGG   +K V A MAS   NR AFI+SSI +AR  +F GLDL W +PS+ 
Sbjct: 74  KNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPSND 133

Query: 127 DEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTIIQTLDWVN 186
            EM  F  ++ EW  AV++E+   ++  L+L A V   P   +  +YP+  I   LD+VN
Sbjct: 134 VEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPD-YYGEEYPVQAIADNLDFVN 193

Query: 187 LISYDFYTPTSSSNFTGPSSAFCNP-RANSLSVNSGIEAWIKCYPHLPSRKIVFGIPFHG 246
           +++YDFY P   S  TGP +A  +P      S +SG+  W++    LP++K V G  + G
Sbjct: 194 IMAYDFYGP-GWSPVTGPPAALFDPSNPAGRSGDSGLSKWLEA--KLPAKKAVLGFSYCG 253

Query: 247 WAWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRKDESYVIGYT 306
           WAW L +   +   +  +G A    ISSDG I Y  I  +I DN A     D + +  Y 
Sbjct: 254 WAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDNGAATFH-DPAVIGFYC 313

Query: 307 FYDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAAD 341
           +  +TWI Y++  +I  K+R AK   + GYF+W++ AD
Sbjct: 314 YVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

BLAST of Tan0019099 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 227.6 bits (579), Expect = 1.5e-59
Identity = 139/361 (38.50%), Postives = 196/361 (54.29%), Query Frame = 0

Query: 7   VHAGYWFTNTNFQ--------LPAANIDANYFTHLFCGHAGIDAQTYEVIIDPMEDDLIY 66
           V A YWF +   Q         P++ ID+  FTHLFC  A +D+ T+EV I         
Sbjct: 14  VKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAANSYQFS 73

Query: 67  EFTPTVRLSNHKVKTLLSIGG---EKDVFAKMASEPTNRDAFIESSISMARAGEFDGLDL 126
            FT TV+  N  V+TLLSIGG   +K V A MAS   NR AFI+SSI +AR  +F GLDL
Sbjct: 74  SFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDL 133

Query: 127 QWFFPSSQDEMDEFENVLSEWHKAVIKEAKDYHRTKLVLVATVSNIPYLQHNIQYPIDTI 186
            W +PS+  EM  F  +L EW  AV++E+   ++  L+L A V   P     ++YP+  I
Sbjct: 134 AWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQYD-GVEYPVKAI 193

Query: 187 IQTLDWVNLISYDFYTPTSSSNFTGPSSAFCNPRAN--SLSVNSGIEAWIKCYPHLPSRK 246
              LD+VN+++YDFY P   S  TGP +A  +  +N    S NSG+  W+     LP +K
Sbjct: 194 ADNLDFVNIMAYDFYGP-GWSPVTGPPAALFHDPSNPAGRSGNSGLRKWLD-EAKLPPKK 253

Query: 247 IVFGIPFHGWAWKLANGHQHQVFSKANGPAIGDNISSDGRIDYYNIIKFIEDNQAENVRK 306
            V G P+ GWAW L +   +   +  +G A    IS DG I Y  I  +I DN A     
Sbjct: 254 AVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIRNYIVDNGAATFH- 313

Query: 307 DESYVIGYTFYDSTWIAYENEATIPEKIRLAKSHHVHGYFAWNIAADDDVNTLANAAQSS 355
           D + +  Y +  +TWI Y++  +I  K++ AK   + GYF+W++ AD +   L+ AA  +
Sbjct: 314 DPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGADYNCG-LSRAASIA 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818626.7e-6841.81Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1[more]
A0A1B1J8Z27.2e-4633.42Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2[more]
U5N4E31.4e-4433.15Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2[more]
A0A072UR651.1e-4332.31Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1[more]
A0A072VEP09.1e-4131.39Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022143191.16.9e-13969.91chitotriosidase-1-like [Momordica charantia][more]
KAG7030350.14.7e-11960.63Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022946077.18.0e-11960.63acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mamm... [more]
KAG6599362.18.0e-11960.63Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1... [more]
XP_023545402.11.4e-11860.34acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1CPI63.4e-13969.91chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013120 PE=4 SV=1[more]
A0A6J1G2P43.9e-11960.63acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE... [more]
A0A6J1DYG44.3e-11860.46chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 S... [more]
A0A6J1KFT31.2e-11759.77chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1[more]
A0A6J1KB821.6e-11759.77chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G19810.14.7e-6941.81Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19800.16.4e-6641.38Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19820.11.9e-6541.00Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19750.14.0e-6039.64Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19760.11.5e-5938.50Glycosyl hydrolase family protein with chitinase insertion domain [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011583Chitinase IISMARTSM006362g34coord: 6..340
e-value: 1.8E-66
score: 236.9
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 9..340
e-value: 6.3E-60
score: 203.5
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 6..354
score: 46.298531
IPR029070Chitinase insertion domain superfamilyGENE3D3.10.50.10coord: 240..311
e-value: 7.9E-81
score: 273.9
IPR029070Chitinase insertion domain superfamilySUPERFAMILY54556Chitinase insertion domaincoord: 241..311
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 23..341
e-value: 7.9E-81
score: 273.9
NoneNo IPR availablePANTHERPTHR11177CHITINASEcoord: 4..348
NoneNo IPR availablePANTHERPTHR11177:SF351CHITINASE-LIKE PROTEIN-RELATEDcoord: 4..348
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 8..345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0019099.1Tan0019099.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0008061 chitin binding
molecular_function GO:0016787 hydrolase activity