Tan0019036 (gene) Snake gourd v1

Overview
NameTan0019036
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionvesicle-associated protein 2-2-like isoform X2
LocationLG09: 32686352 .. 32692256 (+)
RNA-Seq ExpressionTan0019036
SyntenyTan0019036
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTATTTATACTCTAAATAATTCCAAAACAAAAACTTCATTTCCTCGTCGTTTCACTGTCTCTTCTTCGTCCACAACACGGACAGGTTTCATTCTCTTTCCGAATCGCTTCTATTCTTCTTCACTTTTCTTTATCCCTTTTCCGATCTCTTTCACGCCTTTGGATTTATATGATGAAACTCTGTTCGCTGCTTTTTCTTTTCCCCTTTTTTTAATCAGAATATTCCATAACTTTGTGCTTTACAATGGACTGGACTGTGTTTTGTTCGCATGGCGTTCCAAATACTGACCCAGTTCATCCACTCATCCACCCTTTTCTTCTTCTTTATTCAACTTTTTGAGTTGATCGGTTGAATTCTGGTGATTGAGCGAACTCATTGTGTGATCATTCTAGAAAATTTCTTGTTGATCGCGAGTTTTTATACATTCAACTGTATAGAGCTGAATTACGAACATTACAATAGTTTAGTCTGAAAGTGATTTTAGATTTTTTAGTCATTGTTTATTGTAGAAATCCTTAGGGTTTTTTATTTTTTTTAAAAAAATATTTGATGGGGTTCGAATAATTTAATTCGCTTGTTTGTTCGCATATGGATGCACGCCTTTTGTTATTTTGTTTATTCTCGGCTTCAATGATTAAGTTCGATTTGTGCCTTTTCTTTACACAGAATACACTACTCCCCAAAAGTCTCTGTTTTGATCTTTCTTGTCTTTTGTAATTCGATTGTGCAGGGAAATTGTTTGATTCAATAGCGGAGTGAACTGCTTTTCAATTTGAGTTAAGTATGGATATGAGGCTTCTGGAAGTTCATCCTCAGGAATTAAAGTTCATTGGTAAGTCTACAGAATGAATCAATTTTTCTCTTTAAAATTTTGATTTGTTGTTTTCAATGGGCTGTTTGCCGATTATCTCTGGAAACATCACTGGAAATTAGTTGCTGCTCTCTTTCCTTCCTATGAACCGTTTGATCATTTGTGTCAAAAGAAAAACGATTTTCTTGTTTGGATGTTTTTAAGTACTTTATAGCTTTTGAATTTATGTTTTATTCTCTCATTTGTACTCTGTATGCTAGTGATCCCAGCTGAAGGGTGAGTTTGGGATTTCTAGAATGGTAAGGAAGTGCCTTTGGGTAAATAACAATCTTTTTCAAATTTTTGTTGGTATTTTGGTTGTATAGGACAACATGTTTTTATAAATTCAAAAGACACTTAAAAAGTACTTTTAGAAGCACTTAAATGACTTTTGGATGTAGTGCTTTTAGGCAGAACACATTTTTGTAAGCACTAAAAAGGTTTGCTTTTGAAGTATTATAGCACACTTTTCCAATAATTTTAAAAGCACTTTTGACTTAATCAGTCCCCGTAAATTTTAAAAAGTATTTTTAATTAGTTAAAAGTATTTACACTCTTCTAAAAGTGATATTAAACTCAACTTTAGTGCTTTTCCTAAAAGTACTTATTAAAAGTTATCCCAAGCTCACTTTTGTGCGATCAGTATGAAATGCATTGATGTATAAGATTATCTATGATGTCTGCTCTGGTATTTAATTACTAAGCTATATCAAATGAATTGTCATGTCGTGTCTGTTTTTCTAAATATATTTTCTGATTTGCTACTAGGACATTGATTAGTTTAGATTTTTTTTTTTTTTTATGAACAGGGTTTTGTATGTTTCCATTTCGTTTTGCATTCACTTCCTTTTCCAAATCTGAACTAATATCGTTCATATTTTCCTTCTATTGCCTCCAGTTGAACTAAATAAACATAGCTCGTGCTCAATTCAACTAACAAATAATACGCTCGGGCATGTTGCTTTTAAGGTATGTATCAATCATTCTCTGTCTCTGTCTCTTTTTCTTCTCCCTCTCTTTTACTTTTTTTTAAAAAAAAATAAAATTCTATTAAACTGATTTACAACACATTATGTAGAGATAACATGTTTAGTTGCATGCAAGATAATGGACAGAGTTTCTTTTTTAGGTTAAGACCACATCCCCCAAGAATTACTATGTCCGACCAAAGGTCGGAATCATCGAGCCAAACTCAACTCGTGAATTCACAGGTATTTACTTCTGACATCTAGTTAAGGCTTTATCTTTTCTTTCATTTTTGTCCAGGAGGGGAAGGGGGGATGTATGCATGGTTATTTATTTATTGATGTTGAAACATTGAAGTCATCAGTCTTCTTCCATCCTAAGTAGCTTTAATGTGGCTTGATTGAGGCTATATTCTTCTTCTCCTTCTAGCATCTCTGCTTGATTAGCTTTATCATTATTTTTTCTATGCTTCCGTTTCATGTCATAGTCTATTTGATGTACTTCTATATTTGTTCTACTTATCATTATGAAACTGTAAAACTCTTTTTTTCATTGTTTCCATCTTAAGGTAGTATGGCCTGTCTAGTTTGACATGCCTAAGATCTTTAGTTCAATAATTATATTGATCATTTTGCAATGGAATTAATTGTTCATGATGAAATACTTGATGTGTTGATAATTTTCTAACTGATATTTTGCATCCTTATCTTCTATTTTTCTTTCTTATCTCAATCCAACTACTTTGACATCAGTGATTGGCTCAAGTGCTTGCAATAATCATCGCTATCCTTTGTCTTATCATACTGCTCACATTCTTTGATCATCCCTTTCTCTGTTTCTTTTTAAGTTCTGCACTTGTCTAAATTTTTCATGCTTTCAAAATTTTATATAAATGTTCTTAATGTAGTTAGGATGCAAGCACAAAAGATGGCTCCACCAGATGTGCTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGAGGACATAACCTCTAACTTGGTAAATGCTGTTAGTACTATTTATGTTATATAATTGGCTATCTATTATGGCATTTAACATTTTTATTGTTTTTTTTTAGTTTGCCAAAGATGGTGGGAAGTACATAGATGAACAGAAGTTGGGTGTAACCGTTGTTAGCCCATCCAATTCCCCAGCGTTTTCCCCAATTGATGAAGCATTAAAGGCAGAAGTGGCTCTTGGAGTTCCAGAACCAAATTATCTATGTACAGCAGTAGAGGAGATTGTAGACCAGGAACCTTCAAACAAAAACGATGAAATGTCTAATGGAGTTGATATGCTGCCTCCAAAATGCATGGTTAAAATTTCTCAACACATGTCCTTTCTTTTTGCTTGGTGTTCAAATTGCATGTACTTTTATCCTGAGTGGAGTAGAATACTTAGATTAGCATTCATGATAGAGTGTTTTGAGAATGCATTGGAGGCTTAGAGCATGAAAACTGCATAACGCATTCAACTCTAGCACATCACAATAGCTAAATGTTTTGTTGAATCTGTGCTGAATTGAATATCTCGTCCAATCTAGTTGTAACCACTTTTCTTTGCTTATCGAACTACAAAATTTCGGCTGTCCTATGCATTGTACCAGGTTCCTGGTGCTCCTATGGAGCCTAGAGATGTCAATGAAGAGGAGCCTGAACCACCAAGTTTAGCAGACGACTTCACAGATGGCAATGAGGAGGAACTGGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAACTGAGTGAACTCGAATTAAAACTTTGTCAGGTCAATCTTCACAAAATATAGCTCATGATGAAGTGGAAAAACTGTCCAAAAACCATATTTTACTGTTAATTGAAAAGGTTCTGCTAAATGGTAATGTGAATTCATTAACTTTCTTTACAGGCTCAAAATGCCATTTCGAAGCTAAAGGAGGAGAAGAAAATAAGCATCCAAGAAACCAAATTCTTGCAGGAGAATTTTGTAATTTCCTATACCATTTTATTTTCTCTCGAATGTATACTTTCTTATGTTACATCACAAGGCCGATTTCTTTTATTGTTGCCTAGGGGTGTTCAAAAAACCCAATAACCCGACCAACCCGACCAACCCAAACCAACCCGACAGGTTGGGTTGGGTTATGAATTTATTTGATTTGGGTTGGGTTCAAATAAATTAAAATGTATGAGTTGGGTTGGTTCGTGAGTTCACCTAAAATTAATTTGGGTTGACCCGACCAACCCGAACTTATTATTAATTTTAAAAATTATCTTTTTACCTACCATACAACTATATATACATGTATTTAATTTATTTTTATTTAATTTCATAATTATTTATTATTTAATTGCTCTTAAAATACATTTTCAAGTCGTTAGGGTGTAATTTTTCATAATATAAATCAAAACTAAGTGGCTAATTTTAATTGAAAAACAAAGAGTTTTTGTTTTAAATGATGACGATTTATTTAAATGTTTCTTGGGATAATTGTAAATTTGTAATAGAATATTTATGTTTTATGATTGTTATTTAGTATAAAGACATTTGGAGTTTTTTAATTCTTGAATTTTTTATATTTATATTAATTTTTTATTTTTTAATTTTTATTTTTTATTTTAAAAAAAAGATAAAAAAAAAGTTAACCCGATGACCCAACCCAACCCAACCCGAAAATGTTGGGTTGGGTTACCCTCATGAATAGGGTTATTTGGGTTGAAAGAATGACCAACCCGAAAAATCGGGTTGGTCCAAAAAACTAGCATGACCCGACCCAACCCGGCTTATATACACCCCTATGTTGCCTATGATATAACATGAAACGAGATCTGACGACAGTTTATAATTTTGAAACTGTCAGTCAGCTGAAGTCCAAAAATAGTTTTTTCTGTCAATATCCTTAGATCCCTAAACGAACTTTCCTCCAAGTTTTCTAAATTTAATCCATGAACTTTGATAGTGATATCTAGTACGACCTTACTTGAACAGGATCTGTTCTATAGGTTGTATAATTGTGTCGTTGATAGTGATATAGTTAGTCCCTAATTATACCTCTAAACTTTGAATTATGGATTATGACGCAGTGGTTAATGAATAAATGTTGGCTGCTTTACCAGAATATGGCAGACAAATGATTAAGGGAGTGGATAGTAGGAGAAAATAAGGCGTCATGGTCTATTAAAGAGATTTGCTGATACATTGCTTCAACTATTAGACATATTGCTTTATTTTTTCTCGGTGTGTAAGGGGTCATGTCAAATCAGCCCATGTTTTTTTTCCCTCATGGGTATGCCAATATCATCATTTAGTTAATGTTGTTATTAGAAACTACATAGATTACAGTTTCAAAAGTTCAATTCAGATACATAATTGAAAATTTTTAAAATTCAAGGACTGAAAAGAAGATTGAACTTTGAAATATTGTACCAATACCTGTTACGTCCCGAGGATGGTCGGTTCCAGCCGATACCAAACTATCTAACCATTGGCTCCTTTGCCCCAACCAAAGTCCAAGAATGCGGAGCCATTTTTTGTTGGATTCTTGTTAAATTGAACATCTGATGAGCAATATGATTATATGTTCTAGTTTTATTGCTGCAAATTTGTTGAACTATGCTTTGCTTTGTAACTCAAATTCATGAGGGCTGTGCATTTTTTTTTTGTTTGAATTTTTGCAGTCAGAGTTGAGGAAGACAGAGCTCAAAAGTGTTCAAGTTGGGTTTCCTGTTCTATATCTTTACATGGTGGCACTTGTATGTACTTTTCTTGGACGCCTCTTGAGGTAATAAAGATATTTAGTGTTATTATGTGAGTTTCATCTACGGCATAATTTCAAAGGAGGAATTGGTATATAGCTTGAAGTTAGAACAATTTTATGGGTTCTATTCCTCTCGTAGGGTGATCGAGTGTGTAATTCTCTCCTTATATAAGTGGGGTTCGATACTGTTGCTGTGAATTTGAGCATAACTTAAGTAGTTTGATATTAATGCCTTTTCCAACTTGAGTATAGCGTAAGTGGCAAGGCATTAATGTCTCTCATAATGAATGTTCTATGTAATATTGTAAATAATAACAATAAAA

mRNA sequence

ATTTATTTATACTCTAAATAATTCCAAAACAAAAACTTCATTTCCTCGTCGTTTCACTGTCTCTTCTTCGTCCACAACACGGACAGGGAAATTGTTTGATTCAATAGCGGAGTGAACTGCTTTTCAATTTGAGTTAAGTATGGATATGAGGCTTCTGGAAGTTCATCCTCAGGAATTAAAGTTCATTGTTGAACTAAATAAACATAGCTCGTGCTCAATTCAACTAACAAATAATACGCTCGGGCATGTTGCTTTTAAGGTTAAGACCACATCCCCCAAGAATTACTATGTCCGACCAAAGGTCGGAATCATCGAGCCAAACTCAACTCGTGAATTCACAGTTAGGATGCAAGCACAAAAGATGGCTCCACCAGATGTGCTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGAGGACATAACCTCTAACTTGTTTGCCAAAGATGGTGGGAAGTACATAGATGAACAGAAGTTGGGTGTAACCGTTGTTAGCCCATCCAATTCCCCAGCGTTTTCCCCAATTGATGAAGCATTAAAGGCAGAAGTGGCTCTTGGAGTTCCAGAACCAAATTATCTATGTACAGCAGTAGAGGAGATTGTAGACCAGGAACCTTCAAACAAAAACGATGAAATGTCTAATGGAGTTGATATGCTGCCTCCAAAATGCATGGTTCCTGGTGCTCCTATGGAGCCTAGAGATGTCAATGAAGAGGAGCCTGAACCACCAAGTTTAGCAGACGACTTCACAGATGGCAATGAGGAGGAACTGGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAACTGAGTGAACTCGAATTAAAACTTTGTCAGGCTCAAAATGCCATTTCGAAGCTAAAGGAGGAGAAGAAAATAAGCATCCAAGAAACCAAATTCTTGCAGGAGAATTTTTCAGAGTTGAGGAAGACAGAGCTCAAAAGTGTTCAAGTTGGGTTTCCTGTTCTATATCTTTACATGGTGGCACTTGTATGTACTTTTCTTGGACGCCTCTTGAGGTAATAAAGATATTTAGTGTTATTATGTGAGTTTCATCTACGGCATAATTTCAAAGGAGGAATTGGTATATAGCTTGAAGTTAGAACAATTTTATGGGTTCTATTCCTCTCGTAGGGTGATCGAGTGTGTAATTCTCTCCTTATATAAGTGGGGTTCGATACTGTTGCTGTGAATTTGAGCATAACTTAAGTAGTTTGATATTAATGCCTTTTCCAACTTGAGTATAGCGTAAGTGGCAAGGCATTAATGTCTCTCATAATGAATGTTCTATGTAATATTGTAAATAATAACAATAAAA

Coding sequence (CDS)

ATGGATATGAGGCTTCTGGAAGTTCATCCTCAGGAATTAAAGTTCATTGTTGAACTAAATAAACATAGCTCGTGCTCAATTCAACTAACAAATAATACGCTCGGGCATGTTGCTTTTAAGGTTAAGACCACATCCCCCAAGAATTACTATGTCCGACCAAAGGTCGGAATCATCGAGCCAAACTCAACTCGTGAATTCACAGTTAGGATGCAAGCACAAAAGATGGCTCCACCAGATGTGCTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGAGGACATAACCTCTAACTTGTTTGCCAAAGATGGTGGGAAGTACATAGATGAACAGAAGTTGGGTGTAACCGTTGTTAGCCCATCCAATTCCCCAGCGTTTTCCCCAATTGATGAAGCATTAAAGGCAGAAGTGGCTCTTGGAGTTCCAGAACCAAATTATCTATGTACAGCAGTAGAGGAGATTGTAGACCAGGAACCTTCAAACAAAAACGATGAAATGTCTAATGGAGTTGATATGCTGCCTCCAAAATGCATGGTTCCTGGTGCTCCTATGGAGCCTAGAGATGTCAATGAAGAGGAGCCTGAACCACCAAGTTTAGCAGACGACTTCACAGATGGCAATGAGGAGGAACTGGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAACTGAGTGAACTCGAATTAAAACTTTGTCAGGCTCAAAATGCCATTTCGAAGCTAAAGGAGGAGAAGAAAATAAGCATCCAAGAAACCAAATTCTTGCAGGAGAATTTTTCAGAGTTGAGGAAGACAGAGCTCAAAAGTGTTCAAGTTGGGTTTCCTGTTCTATATCTTTACATGGTGGCACTTGTATGTACTTTTCTTGGACGCCTCTTGAGGTAA

Protein sequence

MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEPNSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKLGVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDMLPPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLLR
Homology
BLAST of Tan0019036 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-49
Identity = 149/385 (38.70%), Postives = 200/385 (51.95%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           M+M LL++ P+ L+F V+L K +SC +QLTN T  +VAFKVKTTSPK Y VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
            ST EFTV MQA K  PPD++CKDKFLIQST V A+TTDEDIT+++F+K  GK+I+E KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVE---------EIVDQEPSNKND 180
            VT+V PS+SP  SPI+   +  V       + L +  E         EIV +     +D
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 EM---SNGVDMLPPKCMVP----------------------------------------- 240
           E+    N  ++  PK                                             
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 ----------GAPME-----PRDVN-------EEEPEPPSLAD----DFTDGNE----EE 300
                      APM+     PRD         + EP+     D      TDG      +E
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

Query: 301 LEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKKISIQETKFLQENFSELR-KTELKS 302
           L+++K +EE+KLK+  LE KL QA + ISKL EE+ IS Q  + LQ   +ELR K  +K 
Sbjct: 301 LKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAELRTKKIVKE 360

BLAST of Tan0019036 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.8e-29
Identity = 97/297 (32.66%), Postives = 138/297 (46.46%), Query Frame = 0

Query: 5   LLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEPNSTR 64
           LL V P +L+F  EL K  SCS+ LTN T  +VAFKVKTT+PK Y VRP  G++ P ST 
Sbjct: 22  LLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRSTC 81

Query: 65  EFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKLGVTV 124
           E  V MQAQK AP D+ CKDKFL+Q  +     T +++T  +F+K+ G  ++E KL VT 
Sbjct: 82  EVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVTY 141

Query: 125 VSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDMLPPKC 184
           V+P   P  SP+ E                                              
Sbjct: 142 VAPPRPP--SPVHEG--------------------------------------------- 201

Query: 185 MVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQAQNA 244
                       +EE   P +   D  +G+  E    + + + K    E      +A+  
Sbjct: 202 ------------SEEGSSPRASVSD--NGHGSEFSFERFIVDNKAGHQE---NTSEARAL 254

Query: 245 ISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLLR 302
           I+KL EEK+ +IQ    LQ    +LR+   KS   G P +Y+ +V L+   LG +++
Sbjct: 262 ITKLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIMK 254

BLAST of Tan0019036 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.3e-29
Identity = 61/127 (48.03%), Postives = 87/127 (68.50%), Query Frame = 0

Query: 4   RLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEPNST 63
           +L+ + P ELKF+ EL K S C +++ N T  +VAFKVKTTSPK Y+VRP  G+I+P  +
Sbjct: 8   QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDS 67

Query: 64  REFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKLGVT 123
               V +QAQ+  PPD+ CKDKFL+QST+VP  T  +++  + F KD GK + E KL V+
Sbjct: 68  CIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVS 127

Query: 124 VVSPSNS 131
            ++PS +
Sbjct: 128 YITPSTT 134

BLAST of Tan0019036 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 8.8e-29
Identity = 102/297 (34.34%), Postives = 141/297 (47.47%), Query Frame = 0

Query: 5   LLEVHPQELKFIVELNKHSSCSIQLTNNTLGH-VAFKVKTTSPKNYYVRPKVGIIEPNST 64
           L+ +HP ELKF  EL K SSCS+QLTN T    VAFKVKTT+P+ Y VRP  G++ P  +
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 65  REFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKLGVT 124
              TV MQAQK AP D+ CKDKFL+Q+ VV   TT +++ + +F K+ G+ I++ KL V 
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 125 VVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDMLPPK 184
            + P+N P  SP+ E        G  E N    ++ +I  Q                   
Sbjct: 126 YI-PANPP--SPVPE--------GSEEGNSPMASLNDIASQS------------------ 185

Query: 185 CMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQAQN 244
                                SL DD +   EE  E                 K  +A +
Sbjct: 186 --------------------ASLFDDVSRTFEETSE-----------------KSSEAWS 236

Query: 245 AISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLL 301
            ISKL EEK  + Q+++ L+     LRK   K    G  +L + +V L+   +G LL
Sbjct: 246 MISKLTEEKTSATQQSQKLRLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIGYLL 236

BLAST of Tan0019036 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-28
Identity = 93/301 (30.90%), Postives = 136/301 (45.18%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           M   LL + P +L+F  EL K  SCS+ L N T  +VAFKVKTT+PK Y VRP  G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
            S+ E  V MQAQK AP D+ CKDKFL+Q  V     T +D+T  +F+K+ G  ++E KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
            V  V+P   P  SP+ E                               ++E S+     
Sbjct: 121 RVVYVAPPRPP--SPVREG------------------------------SEEGSS----- 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
                 P A +       +    P  + D  D  +   E                     
Sbjct: 181 ------PRASVSDNGNASDFTAAPRFSADRVDAQDNSSE--------------------- 237

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLL 300
           A+  ++KL EEK  ++Q    LQ+   +LR+   +S   G P +Y+ +V L+   LG ++
Sbjct: 241 ARALVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYIM 237

Query: 301 R 302
           +
Sbjct: 301 K 237

BLAST of Tan0019036 vs. NCBI nr
Match: XP_022968769.1 (vesicle-associated protein 2-2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 453.4 bits (1165), Expect = 1.6e-123
Identity = 245/301 (81.40%), Postives = 263/301 (87.38%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           MDMRLLE+HPQELKFI E NKHS CSIQLTNN L HVAFKVKTTSP  Y+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLEHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
           NSTREFTVRMQAQK+A PD+LCKD+FLIQSTVVPADTTDEDITS+LFAKDGGKYI+EQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
           GVT+VSPS       I +ALK E ALGV  PN L TAVEEIVDQEPS+++  MSNGVD L
Sbjct: 121 GVTLVSPS-------ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSL 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
           PPK MVPG   +PRDV EEEPEP SLA+D  DG+ E LE+IK LEELKLKLSELELKLC+
Sbjct: 181 PPKHMVPGNGTDPRDVGEEEPEPASLANDVIDGDLENLEIIKDLEELKLKLSELELKLCE 240

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLL 300
           AQNAISKLKEEK+ISIQE KFLQENFSELRKTELK VQVGFPVLYLYMVALVCTFLGRLL
Sbjct: 241 AQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRLL 294

Query: 301 R 302
           R
Sbjct: 301 R 294

BLAST of Tan0019036 vs. NCBI nr
Match: XP_023545303.1 (vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545304.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545305.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545306.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545307.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545308.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.0 bits (1164), Expect = 2.0e-123
Identity = 244/301 (81.06%), Postives = 262/301 (87.04%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           MDMRLLE+HPQELKFI E NKHS CSIQLTNN L HVAFKVKTTSP  Y+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
           NSTREFTVRMQAQK+A PD+LCKD+FLIQSTVVPADTTDEDITS+LFAKDGGKYI+EQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
           GVT+VSPS       I +ALK E ALGV  PN L TAVEEIVDQEPS+++  MSNGVD L
Sbjct: 121 GVTLVSPS-------ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSL 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
           PPK MVPG   +PRDV EEEPEP SLA+D  DG+ E LEMIK LEELKLKLSELELKLC+
Sbjct: 181 PPKHMVPGNGTDPRDVGEEEPEPASLANDVVDGDLENLEMIKDLEELKLKLSELELKLCE 240

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLL 300
           AQN+ISKLKEEK+ISIQE KFLQENFSELRKTELK VQVGFPVLYLYMVALVCTFLGR L
Sbjct: 241 AQNSISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRFL 294

Query: 301 R 302
           R
Sbjct: 301 R 294

BLAST of Tan0019036 vs. NCBI nr
Match: XP_023545310.1 (vesicle-associated protein 2-2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.0 bits (1164), Expect = 2.0e-123
Identity = 244/301 (81.06%), Postives = 262/301 (87.04%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           MDMRLLE+HPQELKFI E NKHS CSIQLTNN L HVAFKVKTTSP  Y+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
           NSTREFTVRMQAQK+A PD+LCKD+FLIQSTVVPADTTDEDITS+LFAKDGGKYI+EQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
           GVT+VSPS       I +ALK E ALGV  PN L TAVEEIVDQEPS+++  MSNGVD L
Sbjct: 121 GVTLVSPS-------ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSL 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
           PPK MVPG   +PRDV EEEPEP SLA+D  DG+ E LEMIK LEELKLKLSELELKLC+
Sbjct: 181 PPKHMVPGNGTDPRDVGEEEPEPASLANDVVDGDLENLEMIKDLEELKLKLSELELKLCE 240

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLL 300
           AQN+ISKLKEEK+ISIQE KFLQENFSELRKTELK VQVGFPVLYLYMVALVCTFLGR L
Sbjct: 241 AQNSISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRFL 294

Query: 301 R 302
           R
Sbjct: 301 R 294

BLAST of Tan0019036 vs. NCBI nr
Match: KAG7023965.1 (Vesicle-associated protein 2-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 452.6 bits (1163), Expect = 2.7e-123
Identity = 244/301 (81.06%), Postives = 262/301 (87.04%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           MDMRLLE+HPQELKFI E NKHS CSIQLTNN L HVAFKVKTTSP  Y+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
           NSTREFTVRMQAQK+A PD+LCKD+FLIQSTVVPADTTDEDITS+LFAKDGGKYI+EQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
           GVT+VSPS       I +ALK E ALGV  PN L TAVEEIVDQEPS+++  +SNGVD L
Sbjct: 121 GVTLVSPS-------ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVVSNGVDSL 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
           PPK MVPG   +PRDV EEEPEP SLA+D  DG+ E LEMIK LEELKLKLSELELKLC+
Sbjct: 181 PPKHMVPGNGTDPRDVGEEEPEPASLANDVVDGDLENLEMIKDLEELKLKLSELELKLCE 240

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLL 300
           AQNAISKLKEEK+ISIQE KFLQENFSELRKTELK VQVGFPVLYLYMVALVCTFLGR L
Sbjct: 241 AQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRFL 294

Query: 301 R 302
           R
Sbjct: 301 R 294

BLAST of Tan0019036 vs. NCBI nr
Match: XP_022961473.1 (vesicle-associated protein 2-1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 451.8 bits (1161), Expect = 4.5e-123
Identity = 244/301 (81.06%), Postives = 261/301 (86.71%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           MDMRLLE+HPQELKFI E NKHS CSIQLTNN L HVAFKVKTTSP  Y+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
           NSTREFTVRMQAQK+A PD+LCKD+FLIQSTVVPADTTDEDITS+LFAKDGGKYI+EQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
           GVT++SPS       I +ALK E ALGV  PN L TAVEEIVDQEPS+++  MSNGVD L
Sbjct: 121 GVTLISPS-------ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSL 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
           PPK MVPG    PRDV EEEPEP SLA+D  DG+ E LEMIK LEELKLKLSELELKLC+
Sbjct: 181 PPKHMVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCE 240

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLL 300
           AQNAISKLKEEK+ISIQE KFLQENFSELRKTELK VQVGFPVLYLYMVALVCTFLGR L
Sbjct: 241 AQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRFL 294

Query: 301 R 302
           R
Sbjct: 301 R 294

BLAST of Tan0019036 vs. ExPASy TrEMBL
Match: A0A6J1I0M1 (vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467905 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 7.6e-124
Identity = 245/301 (81.40%), Postives = 263/301 (87.38%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           MDMRLLE+HPQELKFI E NKHS CSIQLTNN L HVAFKVKTTSP  Y+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLEHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
           NSTREFTVRMQAQK+A PD+LCKD+FLIQSTVVPADTTDEDITS+LFAKDGGKYI+EQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
           GVT+VSPS       I +ALK E ALGV  PN L TAVEEIVDQEPS+++  MSNGVD L
Sbjct: 121 GVTLVSPS-------ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSL 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
           PPK MVPG   +PRDV EEEPEP SLA+D  DG+ E LE+IK LEELKLKLSELELKLC+
Sbjct: 181 PPKHMVPGNGTDPRDVGEEEPEPASLANDVIDGDLENLEIIKDLEELKLKLSELELKLCE 240

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLL 300
           AQNAISKLKEEK+ISIQE KFLQENFSELRKTELK VQVGFPVLYLYMVALVCTFLGRLL
Sbjct: 241 AQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRLL 294

Query: 301 R 302
           R
Sbjct: 301 R 294

BLAST of Tan0019036 vs. ExPASy TrEMBL
Match: A0A6J1HAA2 (vesicle-associated protein 2-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462041 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.2e-123
Identity = 244/301 (81.06%), Postives = 261/301 (86.71%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           MDMRLLE+HPQELKFI E NKHS CSIQLTNN L HVAFKVKTTSP  Y+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
           NSTREFTVRMQAQK+A PD+LCKD+FLIQSTVVPADTTDEDITS+LFAKDGGKYI+EQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
           GVT++SPS       I +ALK E ALGV  PN L TAVEEIVDQEPS+++  MSNGVD L
Sbjct: 121 GVTLISPS-------ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSL 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
           PPK MVPG    PRDV EEEPEP SLA+D  DG+ E LEMIK LEELKLKLSELELKLC+
Sbjct: 181 PPKHMVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCE 240

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLL 300
           AQNAISKLKEEK+ISIQE KFLQENFSELRKTELK VQVGFPVLYLYMVALVCTFLGR L
Sbjct: 241 AQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRFL 294

Query: 301 R 302
           R
Sbjct: 301 R 294

BLAST of Tan0019036 vs. ExPASy TrEMBL
Match: A0A6J1HBX8 (vesicle-associated protein 2-1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462041 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.2e-123
Identity = 244/301 (81.06%), Postives = 261/301 (86.71%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           MDMRLLE+HPQELKFI E NKHS CSIQLTNN L HVAFKVKTTSP  Y+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
           NSTREFTVRMQAQK+A PD+LCKD+FLIQSTVVPADTTDEDITS+LFAKDGGKYI+EQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
           GVT++SPS       I +ALK E ALGV  PN L TAVEEIVDQEPS+++  MSNGVD L
Sbjct: 121 GVTLISPS-------ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSL 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
           PPK MVPG    PRDV EEEPEP SLA+D  DG+ E LEMIK LEELKLKLSELELKLC+
Sbjct: 181 PPKHMVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCE 240

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLL 300
           AQNAISKLKEEK+ISIQE KFLQENFSELRKTELK VQVGFPVLYLYMVALVCTFLGR L
Sbjct: 241 AQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRFL 294

Query: 301 R 302
           R
Sbjct: 301 R 294

BLAST of Tan0019036 vs. ExPASy TrEMBL
Match: A0A6J1DMN1 (vesicle-associated protein 1-2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021837 PE=4 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 5.4e-122
Identity = 237/302 (78.48%), Postives = 269/302 (89.07%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           M MRLLE+HPQELKF+ ELNKHSSCSIQLTNNTL HVAFKVKTTSPKNY+VRPKVGIIEP
Sbjct: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
            STREFTVRMQAQ+MAPPD+LCKDKFLIQSTVV ADTTDEDITS+LFAKD GKY++E+KL
Sbjct: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
           GVT+VSPS+    SPIDEALK E  +GV EPN LCTAVE+ +D+E SNK+DEM N  D L
Sbjct: 121 GVTLVSPSS----SPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVGDSL 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
           PPK MVP   MEPRDV + EPEP SLADDF +G+EE+L+MIK +EEL+LKL+ELELKLC+
Sbjct: 181 PPKSMVPDNVMEPRDVGKGEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCE 240

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTEL-KSVQVGFPVLYLYMVALVCTFLGRL 300
           A+ AISKLKEEK+ISIQET+FLQ+NFSELRK+EL K VQVGFP+LYLYMVAL+CTFLGRL
Sbjct: 241 AKVAISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRL 298

Query: 301 LR 302
           LR
Sbjct: 301 LR 298

BLAST of Tan0019036 vs. ExPASy TrEMBL
Match: A0A6J1DP93 (vesicle-associated protein 1-2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021837 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.5e-116
Identity = 231/302 (76.49%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           M MRLLE+HPQELKF+ ELNKHSSCSIQLTNNTL HVAFKVKTTSPKNY+VRPKVGIIEP
Sbjct: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
            STREFTVRMQAQ+MAPPD+LCKDKFLIQSTVV ADTTDEDITS+LFAKD GKY++E+KL
Sbjct: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDML 180
           GVT+VSPS+    SPIDEALK E  +GV EPN LCTAVE+ +D+E SNK+DEM N     
Sbjct: 121 GVTLVSPSS----SPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFN----- 180

Query: 181 PPKCMVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQ 240
                VP   MEPRDV + EPEP SLADDF +G+EE+L+MIK +EEL+LKL+ELELKLC+
Sbjct: 181 -----VPDNVMEPRDVGKGEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCE 240

Query: 241 AQNAISKLKEEKKISIQETKFLQENFSELRKTEL-KSVQVGFPVLYLYMVALVCTFLGRL 300
           A+ AISKLKEEK+ISIQET+FLQ+NFSELRK+EL K VQVGFP+LYLYMVAL+CTFLGRL
Sbjct: 241 AKVAISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRL 288

Query: 301 LR 302
           LR
Sbjct: 301 LR 288

BLAST of Tan0019036 vs. TAIR 10
Match: AT1G08820.1 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-50
Identity = 149/385 (38.70%), Postives = 200/385 (51.95%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           M+M LL++ P+ L+F V+L K +SC +QLTN T  +VAFKVKTTSPK Y VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
            ST EFTV MQA K  PPD++CKDKFLIQST V A+TTDEDIT+++F+K  GK+I+E KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVE---------EIVDQEPSNKND 180
            VT+V PS+SP  SPI+   +  V       + L +  E         EIV +     +D
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 EM---SNGVDMLPPKCMVP----------------------------------------- 240
           E+    N  ++  PK                                             
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 ----------GAPME-----PRDVN-------EEEPEPPSLAD----DFTDGNE----EE 300
                      APM+     PRD         + EP+     D      TDG      +E
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

Query: 301 LEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKKISIQETKFLQENFSELR-KTELKS 302
           L+++K +EE+KLK+  LE KL QA + ISKL EE+ IS Q  + LQ   +ELR K  +K 
Sbjct: 301 LKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAELRTKKIVKE 360

BLAST of Tan0019036 vs. TAIR 10
Match: AT1G08820.2 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-50
Identity = 149/385 (38.70%), Postives = 200/385 (51.95%), Query Frame = 0

Query: 1   MDMRLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEP 60
           M+M LL++ P+ L+F V+L K +SC +QLTN T  +VAFKVKTTSPK Y VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKL 120
            ST EFTV MQA K  PPD++CKDKFLIQST V A+TTDEDIT+++F+K  GK+I+E KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTVVSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVE---------EIVDQEPSNKND 180
            VT+V PS+SP  SPI+   +  V       + L +  E         EIV +     +D
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 EM---SNGVDMLPPKCMVP----------------------------------------- 240
           E+    N  ++  PK                                             
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 ----------GAPME-----PRDVN-------EEEPEPPSLAD----DFTDGNE----EE 300
                      APM+     PRD         + EP+     D      TDG      +E
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

Query: 301 LEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKKISIQETKFLQENFSELR-KTELKS 302
           L+++K +EE+KLK+  LE KL QA + ISKL EE+ IS Q  + LQ   +ELR K  +K 
Sbjct: 301 LKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAELRTKKIVKE 360

BLAST of Tan0019036 vs. TAIR 10
Match: AT3G60600.1 (vesicle associated protein )

HSP 1 Score: 131.3 bits (329), Expect = 1.3e-30
Identity = 97/297 (32.66%), Postives = 138/297 (46.46%), Query Frame = 0

Query: 5   LLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEPNSTR 64
           LL V P +L+F  EL K  SCS+ LTN T  +VAFKVKTT+PK Y VRP  G++ P ST 
Sbjct: 22  LLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRSTC 81

Query: 65  EFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKLGVTV 124
           E  V MQAQK AP D+ CKDKFL+Q  +     T +++T  +F+K+ G  ++E KL VT 
Sbjct: 82  EVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVTY 141

Query: 125 VSPSNSPAFSPIDEALKAEVALGVPEPNYLCTAVEEIVDQEPSNKNDEMSNGVDMLPPKC 184
           V+P   P  SP+ E                                              
Sbjct: 142 VAPPRPP--SPVHEG--------------------------------------------- 201

Query: 185 MVPGAPMEPRDVNEEEPEPPSLADDFTDGNEEELEMIKHLEELKLKLSELELKLCQAQNA 244
                       +EE   P +   D  +G+  E    + + + K    E      +A+  
Sbjct: 202 ------------SEEGSSPRASVSD--NGHGSEFSFERFIVDNKAGHQE---NTSEARAL 254

Query: 245 ISKLKEEKKISIQETKFLQENFSELRKTELKSVQVGFPVLYLYMVALVCTFLGRLLR 302
           I+KL EEK+ +IQ    LQ    +LR+   KS   G P +Y+ +V L+   LG +++
Sbjct: 262 ITKLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIMK 254

BLAST of Tan0019036 vs. TAIR 10
Match: AT5G47180.1 (Plant VAMP (vesicle-associated membrane protein) family protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.7e-30
Identity = 61/127 (48.03%), Postives = 87/127 (68.50%), Query Frame = 0

Query: 4   RLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEPNST 63
           +L+ + P ELKF+ EL K S C +++ N T  +VAFKVKTTSPK Y+VRP  G+I+P  +
Sbjct: 8   QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDS 67

Query: 64  REFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKLGVT 123
               V +QAQ+  PPD+ CKDKFL+QST+VP  T  +++  + F KD GK + E KL V+
Sbjct: 68  CIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVS 127

Query: 124 VVSPSNS 131
            ++PS +
Sbjct: 128 YITPSTT 134

BLAST of Tan0019036 vs. TAIR 10
Match: AT5G47180.2 (Plant VAMP (vesicle-associated membrane protein) family protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.7e-30
Identity = 61/127 (48.03%), Postives = 87/127 (68.50%), Query Frame = 0

Query: 4   RLLEVHPQELKFIVELNKHSSCSIQLTNNTLGHVAFKVKTTSPKNYYVRPKVGIIEPNST 63
           +L+ + P ELKF+ EL K S C +++ N T  +VAFKVKTTSPK Y+VRP  G+I+P  +
Sbjct: 8   QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDS 67

Query: 64  REFTVRMQAQKMAPPDVLCKDKFLIQSTVVPADTTDEDITSNLFAKDGGKYIDEQKLGVT 123
               V +QAQ+  PPD+ CKDKFL+QST+VP  T  +++  + F KD GK + E KL V+
Sbjct: 68  CIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVS 127

Query: 124 VVSPSNS 131
            ++PS +
Sbjct: 128 YITPSTT 134

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9DHD71.6e-4938.70Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Q8VZ951.8e-2932.66Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q9LVU12.3e-2948.03Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Q84WW58.8e-2934.34Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q9SHC82.0e-2830.90Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_022968769.11.6e-12381.40vesicle-associated protein 2-2-like isoform X2 [Cucurbita maxima][more]
XP_023545303.12.0e-12381.06vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_... [more]
XP_023545310.12.0e-12381.06vesicle-associated protein 2-2-like isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG7023965.12.7e-12381.06Vesicle-associated protein 2-2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022961473.14.5e-12381.06vesicle-associated protein 2-1-like isoform X3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1I0M17.6e-12481.40vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1HAA22.2e-12381.06vesicle-associated protein 2-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HBX82.2e-12381.06vesicle-associated protein 2-1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1DMN15.4e-12278.48vesicle-associated protein 1-2-like isoform X1 OS=Momordica charantia OX=3673 GN... [more]
A0A6J1DP931.5e-11676.49vesicle-associated protein 1-2-like isoform X2 OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
AT1G08820.11.1e-5038.70vamp/synaptobrevin-associated protein 27-2 [more]
AT1G08820.21.1e-5038.70vamp/synaptobrevin-associated protein 27-2 [more]
AT3G60600.11.3e-3032.66vesicle associated protein [more]
AT5G47180.11.7e-3048.03Plant VAMP (vesicle-associated membrane protein) family protein [more]
AT5G47180.21.7e-3048.03Plant VAMP (vesicle-associated membrane protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 217..265
NoneNo IPR availablePANTHERPTHR10809:SF45VESICLE-ASSOCIATED PROTEIN 2-2coord: 1..267
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 6..109
e-value: 1.8E-27
score: 95.3
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 5..125
score: 28.438299
IPR016763Vesicle-associated membrane-protein-associated proteinPIRSFPIRSF019693VAMP_assoc_protcoord: 1..285
e-value: 8.8E-45
score: 151.8
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..267
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 1..124
e-value: 8.2E-38
score: 131.1
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 5..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0019036.2Tan0019036.2mRNA
Tan0019036.5Tan0019036.5mRNA
Tan0019036.1Tan0019036.1mRNA
Tan0019036.3Tan0019036.3mRNA
Tan0019036.4Tan0019036.4mRNA
Tan0019036.6Tan0019036.6mRNA
Tan0019036.7Tan0019036.7mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane