Tan0018891 (gene) Snake gourd v1

Overview


Name	Tan0018891
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	pectinesterase inhibitor-like
Location	LG06: 14451516 .. 14452064 (-)
RNA-Seq Expression	Tan0018891
Synteny	Tan0018891

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAATTCCATTATTATTGCAGTCTCTTCTCTTCTAGCTTTCGTCCTTTCACTTCTTTTATCCACAATTGTCGTTCCCATGCATGCAGCTTCCTCAAATGACGTTGTTTCTACCATCTGCCCAAAAACTAGAAATCCATCTTTTTGCTCCAATGTCTTGAATTCTGCTCGTACCACGGACCTAAAAGGACTAGCCATTTTCACTCTCAACCTCGCCCACACAAGGGCCGCAAAAAACCGCCTCCTCGTGCGGTCCCTAGCATTGAACGCGACTGATCCAAGGCTTAAGGAGTGGTACGCCTCTTGTGCTGAAAACTACGACAATGTTGTCAGTGACATTGACGATGCAAAGAACTACTTGGCCTCCGGTGACTACGATGGCGTCAACATTCAGGCTTCTGGAGCCATGACGGAGGTCGATGACTGTCAGGACAACTTCACACAACCGCCAATGGACCCATCGGCATTGTCCACCAATGCTAAAGCTCTGGAAGATATTTGTAGTATTATATTGGTTATATCTAATCTTCTTCTTGGACATGCATAA

mRNA sequence

ATGGTGAATTCCATTATTATTGCAGTCTCTTCTCTTCTAGCTTTCGTCCTTTCACTTCTTTTATCCACAATTGTCGTTCCCATGCATGCAGCTTCCTCAAATGACGTTGTTTCTACCATCTGCCCAAAAACTAGAAATCCATCTTTTTGCTCCAATGTCTTGAATTCTGCTCGTACCACGGACCTAAAAGGACTAGCCATTTTCACTCTCAACCTCGCCCACACAAGGGCCGCAAAAAACCGCCTCCTCGTGCGGTCCCTAGCATTGAACGCGACTGATCCAAGGCTTAAGGAGTGGTACGCCTCTTGTGCTGAAAACTACGACAATGTTGTCAGTGACATTGACGATGCAAAGAACTACTTGGCCTCCGGTGACTACGATGGCGTCAACATTCAGGCTTCTGGAGCCATGACGGAGGTCGATGACTGTCAGGACAACTTCACACAACCGCCAATGGACCCATCGGCATTGTCCACCAATGCTAAAGCTCTGGAAGATATTTGTAGTATTATATTGGTTATATCTAATCTTCTTCTTGGACATGCATAA

Coding sequence (CDS)

ATGGTGAATTCCATTATTATTGCAGTCTCTTCTCTTCTAGCTTTCGTCCTTTCACTTCTTTTATCCACAATTGTCGTTCCCATGCATGCAGCTTCCTCAAATGACGTTGTTTCTACCATCTGCCCAAAAACTAGAAATCCATCTTTTTGCTCCAATGTCTTGAATTCTGCTCGTACCACGGACCTAAAAGGACTAGCCATTTTCACTCTCAACCTCGCCCACACAAGGGCCGCAAAAAACCGCCTCCTCGTGCGGTCCCTAGCATTGAACGCGACTGATCCAAGGCTTAAGGAGTGGTACGCCTCTTGTGCTGAAAACTACGACAATGTTGTCAGTGACATTGACGATGCAAAGAACTACTTGGCCTCCGGTGACTACGATGGCGTCAACATTCAGGCTTCTGGAGCCATGACGGAGGTCGATGACTGTCAGGACAACTTCACACAACCGCCAATGGACCCATCGGCATTGTCCACCAATGCTAAAGCTCTGGAAGATATTTGTAGTATTATATTGGTTATATCTAATCTTCTTCTTGGACATGCATAA

Protein sequence

MVNSIIIAVSSLLAFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTTDLKGLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLASGDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLLLGHA

Homology

[Close]

BLAST of Tan0018891 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 124.8 bits (312), Expect = 1.0e-27
Identity = 73/173 (42.20%), Postives = 101/173 (58.38%), Query Frame = 0


Query: 15  FVLSLLLSTIVVPMHAASS----NDVVSTICPKTRNPSFCSNVLNS---ARTTDLKGLAI 74
           FV  LL+   + P+    S    N ++S ICPKTRNPS C   L S   + + DLKGL  
Sbjct: 11  FVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLKGLGQ 70

Query: 75  FTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLASGDYD 134
           F++++A   A +   ++ SL   ATDP+LK  Y +C+ENY + +  +  AK +L SGDY+
Sbjct: 71  FSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTSGDYN 130

Query: 135 GVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLLLG 181
            +NI AS A      C+D+F  PP  P+ L      LED+C I+LVISNLL G
Sbjct: 131 SLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLLPG 183

[Close]

BLAST of Tan0018891 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 3.8e-19
Identity = 59/166 (35.54%), Postives = 100/166 (60.24%), Query Frame = 0


Query: 14  AFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSA-RTTDLKGLAIFTLNL 73
           AF+ SL+   ++   +A +S++ +STIC KT NPSFC   LN+   + +L+ LA  TL+ 
Sbjct: 9   AFLSSLMFLLLIGSSYAITSSE-MSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDS 68

Query: 74  AHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLASGDYDGVNIQ 133
              RA +    ++S+     DPR K  Y SC + Y++ + ++++A  +LASGD  G+N++
Sbjct: 69  TQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMK 128

Query: 134 ASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLL 179
            S A+   D C D+  +     S++  N+K ++++C I LVISN+L
Sbjct: 129 VSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Tan0018891 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 6.6e-19
Identity = 64/175 (36.57%), Postives = 101/175 (57.71%), Query Frame = 0


Query: 7   IAVSSLLAFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTT---DLK 66
           + +SSL+ FV++  L+  V  + A         IC K +N SFC++ + S   T   DL+
Sbjct: 9   VLMSSLMFFVMTGSLNAQVADIKA---------ICGKAKNQSFCTSYMKSNPKTSGADLQ 68

Query: 67  GLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLAS 126
            LA  T   A T A++    ++SL   AT+P +K+ Y SC ++Y + +S ++DAK  LAS
Sbjct: 69  TLANITFGSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLAS 128

Query: 127 GDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLL 179
           GD  G+NI+ S AM     C+ +     +DPSA+  N+   ++IC I+LVISN++
Sbjct: 129 GDGKGLNIKVSAAMEGPSTCEQDMADFKVDPSAVK-NSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Tan0018891 vs. NCBI nr
Match: XP_038896411.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 248.4 bits (633), Expect = 4.6e-62
Identity = 131/180 (72.78%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 1   MVNSIIIAVSSLLAFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTT 60
           M NSII+  SSLL FVLSLL   +VVPMHAAS NDVVSTIC +TRNP FCSN+L SARTT
Sbjct: 1   MANSIILPASSLLPFVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTT 60

Query: 61  DLKGLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNY 120
           DLKGL  FT+NLAHT+A ++R L + LA  A DP+ KE YASCA++YD+  SD++DAKNY
Sbjct: 61  DLKGLITFTINLAHTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNY 120

Query: 121 LASGDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLLLG 180
           LA+GDYDGVNIQASGAMTE DDCQDNFTQ PMD S LS + KALEDICSIILVI+N+LLG
Sbjct: 121 LATGDYDGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANILLG 180

[Close]

BLAST of Tan0018891 vs. NCBI nr
Match: XP_038896410.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 246.1 bits (627), Expect = 2.3e-61
Identity = 133/180 (73.89%), Postives = 147/180 (81.67%), Query Frame = 0


Query: 1   MVNSIIIAVSSLLAFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTT 60
           M NSII+  SSLL FVLSLL    VVPMHAAS N+VVS IC +T NP FCSN+L SARTT
Sbjct: 1   MANSIILTASSLLPFVLSLLFFHHVVPMHAASQNNVVSIICQRTGNPPFCSNLLKSARTT 60

Query: 61  DLKGLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNY 120
           DLKGLA FTLNLA T A K+R L + LAL A DP+LKE YASCAE+YD+   D+DDAKNY
Sbjct: 61  DLKGLATFTLNLARTNATKSRALAQFLALKAADPKLKERYASCAEHYDDAAGDLDDAKNY 120

Query: 121 LASGDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLLLG 180
           LA+GDY+GVNIQASGAMTE DDCQDNFTQ PMD S LS N KALEDICSIILVI+N+LLG
Sbjct: 121 LATGDYNGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKNGKALEDICSIILVIANILLG 180

[Close]

BLAST of Tan0018891 vs. NCBI nr
Match: XP_023549346.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 229.6 bits (584), Expect = 2.2e-56
Identity = 125/182 (68.68%), Postives = 145/182 (79.67%), Query Frame = 0


Query: 1   MVNSIIIAVSSLLAFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTT 60
           M +S  +AVSSLLAFVLSLL    VVPMHAAS +DVVSTIC KTRNPSFC N L SA TT
Sbjct: 1   MASSSFLAVSSLLAFVLSLLFFNGVVPMHAASPDDVVSTICKKTRNPSFCFNALKSAGTT 60

Query: 61  DLKGLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNY 120
           DLKGLA FTLNLAH +A ++  L +SLA  ATDP+LKE Y++CAE YD+   DI+D K+ 
Sbjct: 61  DLKGLATFTLNLAHDKAVESHALAQSLASKATDPKLKERYSTCAELYDDATDDIEDGKSN 120

Query: 121 LASGDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLLLG 180
           L  GDY+GVNI+AS AMTE DDC D+FTQPP DPSALS N +A+E+ICSIILVI+NLLLG
Sbjct: 121 LREGDYNGVNIKASAAMTEADDCLDSFTQPPKDPSALSGNGEAMENICSIILVIANLLLG 180

Query: 181 HA 183
            A
Sbjct: 181 RA 182

[Close]

BLAST of Tan0018891 vs. NCBI nr
Match: XP_022949197.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 225.7 bits (574), Expect = 3.2e-55
Identity = 114/158 (72.15%), Postives = 130/158 (82.28%), Query Frame = 0


Query: 25  VVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTTDLKGLAIFTLNLAHTRAAKNRLLV 84
           +VPMHAAS ND+VSTIC KTRNPSFC NVL SA TTDLKGLA FTLNLAH +A ++  L 
Sbjct: 1   MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALA 60

Query: 85  RSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLASGDYDGVNIQASGAMTEVDDCQ 144
           +SLA  A DP+LKE YA+CAE YD+ V DI+D KNYL  GDY+GVNI+AS AMTE +DC 
Sbjct: 61  QSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCL 120

Query: 145 DNFTQPPMDPSALSTNAKALEDICSIILVISNLLLGHA 183
           D+FTQPP DPSALS N K +EDICSIILVI+NLLLGHA
Sbjct: 121 DSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 158

[Close]

BLAST of Tan0018891 vs. NCBI nr
Match: XP_023523299.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 225.3 bits (573), Expect = 4.2e-55
Identity = 122/182 (67.03%), Postives = 142/182 (78.02%), Query Frame = 0


Query: 1   MVNSIIIAVSSLLAFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTT 60
           M +S  +AVSS+   VLS++    +VPMHAAS ND+VSTIC KTRNPSFC NVLNSA TT
Sbjct: 1   MASSSFLAVSSI---VLSVMFFNGMVPMHAASENDIVSTICKKTRNPSFCFNVLNSAGTT 60

Query: 61  DLKGLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNY 120
           DLKGLA FTLNLAH +AA++R+L +SLA  ATDP+LKE YA+CAE+YD+ V DI+D KNY
Sbjct: 61  DLKGLATFTLNLAHDKAAQSRVLAQSLASKATDPKLKERYATCAEHYDDAVDDIEDGKNY 120

Query: 121 LASGDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLLLG 180
           L  GDY+ VN  AS AMTE  DC D+F QPP DPS L  N KA+EDICSIILVI NLLLG
Sbjct: 121 LGKGDYNSVNTMASAAMTEAGDCLDSFMQPPKDPSTLFDNGKAVEDICSIILVIGNLLLG 179

Query: 181 HA 183
            A
Sbjct: 181 RA 179

[Close]

BLAST of Tan0018891 vs. ExPASy TrEMBL
Match: A0A6J1GBD1 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111452619 PE=4 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 1.6e-55
Identity = 114/158 (72.15%), Postives = 130/158 (82.28%), Query Frame = 0


Query: 25  VVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTTDLKGLAIFTLNLAHTRAAKNRLLV 84
           +VPMHAAS ND+VSTIC KTRNPSFC NVL SA TTDLKGLA FTLNLAH +A ++  L 
Sbjct: 1   MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALA 60

Query: 85  RSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLASGDYDGVNIQASGAMTEVDDCQ 144
           +SLA  A DP+LKE YA+CAE YD+ V DI+D KNYL  GDY+GVNI+AS AMTE +DC 
Sbjct: 61  QSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCL 120

Query: 145 DNFTQPPMDPSALSTNAKALEDICSIILVISNLLLGHA 183
           D+FTQPP DPSALS N K +EDICSIILVI+NLLLGHA
Sbjct: 121 DSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGHA 158

[Close]

BLAST of Tan0018891 vs. ExPASy TrEMBL
Match: A0A6J1EVZ9 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111438395 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 9.4e-53
Identity = 117/182 (64.29%), Postives = 137/182 (75.27%), Query Frame = 0


Query: 1   MVNSIIIAVSSLLAFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTT 60
           M +S  +AVSS+   VLS+L    +VPMH A  ND+VSTIC KTRNPSFC NVLNSA TT
Sbjct: 1   MASSSFLAVSSI---VLSVLFFNGMVPMHVAFENDIVSTICKKTRNPSFCFNVLNSAGTT 60

Query: 61  DLKGLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNY 120
           DLKGLA FTLNLA+ +AA++R+L +SL   ATDP+ KE YA+C E+YD+ V DI+D KNY
Sbjct: 61  DLKGLATFTLNLAYNKAAQSRVLAQSLVSKATDPKFKERYATCVEHYDDAVDDIEDGKNY 120

Query: 121 LASGDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLLLG 180
           L  GDY+ VN  AS  MTE  DC D+F QPP DPSALS N K +EDICSIILVI NLLLG
Sbjct: 121 LGKGDYNSVNTMASAVMTEASDCLDSFMQPPKDPSALSDNRKVVEDICSIILVIGNLLLG 179

Query: 181 HA 183
            A
Sbjct: 181 RA 179

[Close]

BLAST of Tan0018891 vs. ExPASy TrEMBL
Match: A0A6J1F473 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440170 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 9.4e-53
Identity = 119/179 (66.48%), Postives = 137/179 (76.54%), Query Frame = 0


Query: 7   IAVSSLLA---FVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTTDLK 66
           +A SS LA    VL ++    + PMHAAS +D+VSTIC KTRNPSFC NVL SA TTDLK
Sbjct: 1   MASSSFLAVFRIVLLVMFFNGMAPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 67  GLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLAS 126
           GLA FTLNLAH + A+ R L  SLA  ATDP+LKE YA+CAE YD+   DI+D KN L  
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKATDPKLKERYATCAEQYDDADDDIEDGKNDLGE 120

Query: 127 GDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLLLGHA 183
           GDY+GVNI+AS AMTEV DC D+FTQPP DPSALS+N K +EDICSIILVI+NLLLG A
Sbjct: 121 GDYNGVNIKASAAMTEVGDCLDSFTQPPKDPSALSSNGKTVEDICSIILVIANLLLGRA 179

[Close]

BLAST of Tan0018891 vs. ExPASy TrEMBL
Match: A0A6J1EZI7 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440672 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 2.1e-52
Identity = 119/179 (66.48%), Postives = 135/179 (75.42%), Query Frame = 0


Query: 7   IAVSSLLA---FVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTTDLK 66
           +A SS LA    VL +L    + PMHAAS +D+VSTIC KTRNPSFC NVL SA TTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMAPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 67  GLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLAS 126
           GLA FTLNLAH + A+ R L  SLA   TDP+LKE YA+CAE YD+   DI+D KN L  
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKETDPKLKERYATCAEQYDDAADDIEDGKNDLGE 120

Query: 127 GDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLLLGHA 183
           GDY+GVNI+AS AMTEV DC D+FTQPP DPSALS N K +EDICSIILVI+NLLLG A
Sbjct: 121 GDYNGVNIKASAAMTEVGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGRA 179

[Close]

BLAST of Tan0018891 vs. ExPASy TrEMBL
Match: A0A6J1GPK1 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111456292 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 2.1e-52
Identity = 122/182 (67.03%), Postives = 139/182 (76.37%), Query Frame = 0


Query: 1   MVNSIIIAVSSLLAFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTT 60
           M +S  I VSSLLAFVLSLL S  VVPMHAA +N    TIC KTRNPS C NVL SA TT
Sbjct: 1   MTSSSFIVVSSLLAFVLSLLFSNGVVPMHAAFTNGAPFTICKKTRNPSLCFNVLKSAGTT 60

Query: 61  DLKGLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNY 120
           +LK LA FTLNLA+ +AA++  L +SLA  A DPRLKE YA+C E+Y+NVV  I+ AK+ 
Sbjct: 61  NLKHLATFTLNLANNKAAQSCALAQSLASKAVDPRLKELYATCVEHYNNVVVVIEGAKSN 120

Query: 121 LASGDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLLLG 180
           L  GDY+GVNIQAS AMTE  DC DNFTQPP DPS LS N KA+EDICSI+LVI+NLLLG
Sbjct: 121 LGKGDYNGVNIQASRAMTEASDCLDNFTQPPKDPSTLSGNGKAVEDICSIVLVIANLLLG 180

Query: 181 HA 183
            A
Sbjct: 181 RA 182

[Close]

BLAST of Tan0018891 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 96.3 bits (238), Expect = 2.7e-20
Identity = 59/166 (35.54%), Postives = 100/166 (60.24%), Query Frame = 0


Query: 14  AFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSA-RTTDLKGLAIFTLNL 73
           AF+ SL+   ++   +A +S++ +STIC KT NPSFC   LN+   + +L+ LA  TL+ 
Sbjct: 9   AFLSSLMFLLLIGSSYAITSSE-MSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDS 68

Query: 74  AHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLASGDYDGVNIQ 133
              RA +    ++S+     DPR K  Y SC + Y++ + ++++A  +LASGD  G+N++
Sbjct: 69  TQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMK 128

Query: 134 ASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLL 179
            S A+   D C D+  +     S++  N+K ++++C I LVISN+L
Sbjct: 129 VSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Tan0018891 vs. TAIR 10
Match: AT3G17220.1 (pectin methylesterase inhibitor 2 )

HSP 1 Score: 95.5 bits (236), Expect = 4.7e-20
Identity = 64/175 (36.57%), Postives = 101/175 (57.71%), Query Frame = 0


Query: 7   IAVSSLLAFVLSLLLSTIVVPMHAASSNDVVSTICPKTRNPSFCSNVLNSARTT---DLK 66
           + +SSL+ FV++  L+  V  + A         IC K +N SFC++ + S   T   DL+
Sbjct: 9   VLMSSLMFFVMTGSLNAQVADIKA---------ICGKAKNQSFCTSYMKSNPKTSGADLQ 68

Query: 67  GLAIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLAS 126
            LA  T   A T A++    ++SL   AT+P +K+ Y SC ++Y + +S ++DAK  LAS
Sbjct: 69  TLANITFGSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLAS 128

Query: 127 GDYDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVISNLL 179
           GD  G+NI+ S AM     C+ +     +DPSA+  N+   ++IC I+LVISN++
Sbjct: 129 GDGKGLNIKVSAAMEGPSTCEQDMADFKVDPSAVK-NSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Tan0018891 vs. TAIR 10
Match: AT2G47340.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 7.0e-08
Identity = 27/80 (33.75%), Postives = 47/80 (58.75%), Query Frame = 0


Query: 96  LKEWYASCAENYDNVVSDIDDAKNYLASGDYDGVNIQASGAMTEVDDCQDNFTQPPMDPS 155
           +KE  + C ENYD+++S ++DAK  +  GDY  ++   S A+++V  C DNF   P   S
Sbjct: 197 VKEAMSVCVENYDSLISGLEDAKMAMEEGDYGRLDSVLSAAISDVSTCSDNFVDIPDVDS 256

Query: 156 ALSTNAKALEDICSIILVIS 176
             ++    ++ +CS +L +S
Sbjct: 257 PTASLDDLMKKLCSNVLAMS 276

[Close]

BLAST of Tan0018891 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 5.9e-07
Identity = 43/144 (29.86%), Postives = 67/144 (46.53%), Query Frame = 0


Query: 37  VSTICPKTRNPSFCSNVLNSARTTDLK--GLAIFTLNLAHTRAAKNRLLVRSLALNATDP 96
           + TIC KT NP  C + ++   T  LK    ++  L +  +  A    +     ++A+D 
Sbjct: 130 IKTICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAMAIVEKVDASD- 189

Query: 97  RLKEWYASCAENYDNVVSDIDDAKNYLASGDYDGVNIQASGAMTEVDDCQDNFTQPPMDP 156
                   C E YD+ V +++DA N + S D   VN   S AMT+   C D F +   +P
Sbjct: 190 --------CQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESG-EP 249

Query: 157 SALSTNAKALEDICSIILVISNLL 179
           + L+  A  L  + S  L IS L+
Sbjct: 250 NPLAYVADKLTKMVSNCLAISTLI 263

[Close]

BLAST of Tan0018891 vs. TAIR 10
Match: AT4G03945.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 5.0e-06
Identity = 49/169 (28.99%), Postives = 75/169 (44.38%), Query Frame = 0


Query: 11  SLLAF-VLSLLLSTIVVPMHAASSNDVVSTICPKTR-NPSFCSNVLNS---ARTTDLKGL 70
           SL  F VL L + +     +   + D + TIC K   N S+C  VLN+       D   L
Sbjct: 10  SLACFLVLLLFVDSSYARFNTLVTKDQIHTICTKQDINSSYCFQVLNANPEIARLDFPSL 69

Query: 71  AIFTLNLAHTRAAKNRLLVRSLALNATDPRLKEWYASCAENYDNVVSDIDDAKNYLASGD 130
             F LN    +   ++L    L+   T P ++  Y  C E Y+N     D+A  YLA+ D
Sbjct: 70  FKFVLNY-QAQNISDKLKQFKLSGGYT-PGVESKYNLCIEFYENSFDRRDEALRYLAAKD 129

Query: 131 YDGVNIQASGAMTEVDDCQDNFTQPPMDPSALSTNAKALEDICSIILVI 175
           Y  VN    G    + +C D+ +     P    T +  ++++  I+LVI
Sbjct: 130 YGSVNTMVGGTAINMFNCADDLSTMKPVPQFFVTESNLIKELSKILLVI 176

The following BLAST results are available for this feature:

BLAST of Tan0018891 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	1.0e-27	42.20	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LNF2	3.8e-19	35.54	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]
Q9LUV1	6.6e-19	36.57	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

BLAST of Tan0018891 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896411.1	4.6e-62	72.78	pectinesterase inhibitor-like [Benincasa hispida]	[more]
XP_038896410.1	2.3e-61	73.89	pectinesterase inhibitor-like [Benincasa hispida]	[more]
XP_023549346.1	2.2e-56	68.68	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
XP_022949197.1	3.2e-55	72.15	pectinesterase inhibitor-like [Cucurbita moschata]	[more]
XP_023523299.1	4.2e-55	67.03	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Tan0018891 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBD1	1.6e-55	72.15	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111452619 PE=4...	[more]
A0A6J1EVZ9	9.4e-53	64.29	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111438395 PE=4...	[more]
A0A6J1F473	9.4e-53	66.48	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440170 PE=4...	[more]
A0A6J1EZI7	2.1e-52	66.48	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440672 PE=4...	[more]
A0A6J1GPK1	2.1e-52	67.03	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111456292 PE=4...	[more]

BLAST of Tan0018891 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48020.1	2.7e-20	35.54	pectin methylesterase inhibitor 1	[more]
AT3G17220.1	4.7e-20	36.57	pectin methylesterase inhibitor 2	[more]
AT2G47340.1	7.0e-08	33.75	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G36659.1	5.9e-07	29.86	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G03945.1	5.0e-06	28.99	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 32..174 e-value: 4.0E-26 score: 102.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 11..178 e-value: 9.4E-34 score: 114.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 36..174 e-value: 1.2E-20 score: 74.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 31..178 e-value: 3.2E-36 score: 126.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 34..179
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 8..178
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 8..178
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 34..178 e-value: 2.67638E-46 score: 147.184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0018891.1	Tan0018891.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity