Tan0018867 (gene) Snake gourd v1

BLAST of Tan0018867 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 5.3e-28
Identity = 85/258 (32.95%), Postives = 128/258 (49.61%), Query Frame = 0

Query: 31  TTHLVGLDGLCLDI-SPFVTNYISTVLSPCERTKESQLWTIIKDGTIRPKDIRYCLVPQS 90
           T ++VG DGLC+D+ + + T+     L PC  T+ +Q WT   D TIR   +  C+    
Sbjct: 309 TRNIVGRDGLCVDVRNGYDTDGTPLQLWPC-GTQRNQRWTFDSDDTIR--SMGKCMTANG 368

Query: 91  NMIGTDAILEECDKGRRSDLNIKWTKMKDGTIRHDESGLVLTG-----------KSNKDA 150
              G++ ++  C     ++  IKW    DG+I +  SGLV+T            + N  A
Sbjct: 369 LNNGSNIVIFNCSTA--AENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLEDNIYA 428

Query: 151 PSQSWEATEVFSPTVANIMWLENLCLQSREAVDWHVSLSECNEKDNNQRWAFYGDGTIRQ 210
            SQ W  T    P VA+I+  + +CLQS    +  V + +C      Q+WA YGD TIR 
Sbjct: 429 ASQGWTVTNNVKPIVASIVGYKEMCLQS-NGENNGVWMEDCEATSLQQQWALYGDRTIRV 488

Query: 211 NENRNYCLTSSDRNVGSLVVVSKCGDKPQQRWSLNAEDKTINHPNTNLVMDATRYIGLSP 270
           N  R  C+T++  N   L+++ KC   P QRW  N++   +N P +  VMD  R   +S 
Sbjct: 489 NSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVN-PKSRHVMD-VRASNVSL 548

Query: 271 LPPVIFVDKRDGSSSQQW 277
              +IF     G+ +QQW
Sbjct: 549 REIIIF--PATGNPNQQW 556

BLAST of Tan0018867 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 8.5e-26
Identity = 91/265 (34.34%), Postives = 129/265 (48.68%), Query Frame = 0

Query: 27  VIGGTTHLVGLDGLCLDISPFVTNYIS---TVLSPCERTKESQLWTIIKDGTIRPKDIRY 86
           V+  T  + G DGLC+D+     +YI      L PC   + +QLWT   DGTIR   +  
Sbjct: 318 VVEVTRRISGWDGLCVDVR--YGHYIDGNPVQLRPC-GNECNQLWTFRTDGTIR--WLGK 377

Query: 87  CLVPQSNMIGTDAILEECDKGRRSDLNIKWTKMKDGTIRHDESGLVLTG----------- 146
           CL   S++     ++ +C+         KW    DGTI +  SGLVLT            
Sbjct: 378 CLTASSSV-----MIYDCNTVPPE--ATKWVVSIDGTITNPHSGLVLTAPQAAEGTALSL 437

Query: 147 KSNKDAPSQSWEATEVFSPTVANIMWLENLCLQSREAVDWHVSLSECNEKDNNQRWAFYG 206
           ++N  A  Q W   +V  P V  I+  + +CL+     ++ V L +C      Q WA YG
Sbjct: 438 ENNIHAARQGWTVGDV-EPLVTFIVGYKQMCLRENGENNF-VWLEDCVLNRVQQEWALYG 497

Query: 207 DGTIRQNENRNYCLTSSDRNVGSLVVVSKCGDKPQQRWSLNAEDKTINHPNTNLVMD-AT 266
           DGTIR N NR+ C+TS D     L+V+ KC     QRW  N  + TI++PN  L+MD A 
Sbjct: 498 DGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNT-NGTISNPNAKLLMDVAQ 557

Query: 267 RYIGLSPLPPVIFVDKRDGSSSQQW 277
           R + L      I + +  G+ +QQW
Sbjct: 558 RDVSLRK----IILYRPTGNPNQQW 563

BLAST of Tan0018867 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.5e-25
Identity = 92/268 (34.33%), Postives = 128/268 (47.76%), Query Frame = 0

Query: 27  VIGGTTHLVGLDGLCLDISPFVTNYI---STVLSPCERTKESQLWTIIKDGTIRPKDIRY 86
           V+  T  + G DGLC+D+     +YI   +  L PC   + +QLWT   DGTIR   +  
Sbjct: 318 VVEVTRRISGWDGLCVDVRD--GHYIDGNTVQLGPC-GNECNQLWTFRTDGTIR--WLGK 377

Query: 87  CLVPQSNMIGTDAILEECDKGRRSDLNIKWTKMKDGTIRHDESGLVLTG----------- 146
           CL   S++     ++ +C+         KW    DGTI +  SGLVLT            
Sbjct: 378 CLTTSSSV-----MIYDCNTVPPE--ATKWVVSTDGTITNPRSGLVLTAPQAAEGTALSL 437

Query: 147 KSNKDAPSQSWEATEVFSPTVANIMWLENLCLQSREAVDWHVSLSECNEKDNNQRWAFYG 206
           ++N  A  Q W   +V  P V  I+  + +CL +    +  V L +C      Q WA YG
Sbjct: 438 ENNIHAARQGWTVGDV-EPLVTFIVGYKQMCL-TENGENNFVWLEDCVLNRVEQEWALYG 497

Query: 207 DGTIRQNENRNYCLTSSDRNVGSLVVVSKCGDKPQQRWSLNAEDKTINHPNTNLVMD-AT 266
           DGTIR N NR+ C+TS D     L+V+ KC     QRW  N  + TI++PN  LVMD A 
Sbjct: 498 DGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNT-NGTISNPNAKLVMDVAQ 557

Query: 267 RYIGLSPL---PPVIFVDKRDGSSSQQW 277
             + L  +   PP        G+ +QQW
Sbjct: 558 SNVSLRKIILYPPT-------GNPNQQW 563

BLAST of Tan0018867 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-23
Identity = 75/264 (28.41%), Postives = 123/264 (46.59%), Query Frame = 0

Query: 31  TTHLVGLDGLCLDISPFVTNYIS-TVLSPCERTKESQLWTIIKDGTIRPKDIRYCLVPQS 90
           TT + G D LC+D++  +T+  S  +L PC + + +Q WT   DGT+R   +  CL   +
Sbjct: 264 TTRISGRDALCVDVAGALTSDGSRLILYPCGQ-QVNQKWTFHSDGTVR--SLGKCLATNN 323

Query: 91  NMIGTDAILEECDKGRRSDLNIKWTKMKDGTI---RHDESGLV---------LTGKSNKD 150
           +  G   ++ +C K    D  I W     GTI    +++  L          LT + N  
Sbjct: 324 SKFGNLVVIYDCSKLAAED--ISWDVSVGGTIMNPNYEDLALTSNKATRSTNLTMEVNTY 383

Query: 151 APSQSWEATEVFSPTVANIMWLENLCLQSREAVDWHVSLSECNEKDNNQRWAFYGDGTIR 210
           + SQ W       P + +I+ L+++CL++ +  + ++ L EC      Q WA Y DGTIR
Sbjct: 384 SASQGWRVGNYVQPIIGSIVGLDDMCLEATDG-NTNMWLEECVPNQREQSWALYSDGTIR 443

Query: 211 QNENRNYCLTSSDRNVGS--LVVVSKCGDKPQQRWSLNAEDKTINHPNTNLVMDATRY-I 270
            ++NR  C+T+S     +  ++ +  C     QRW   A+       N  L MD  R  +
Sbjct: 444 VDDNRELCVTASSSTYDNWKVITILNCDGSNNQRWVFLADGSISTPGNQRLAMDVARSDV 503

Query: 271 GLSPLPPVIFVDKRDGSSSQQWTI 279
            L      I + +  G  +QQW +
Sbjct: 504 DLKK----IILHRPHGDLNQQWVL 517

BLAST of Tan0018867 vs. ExPASy Swiss-Prot
Match: P82683 (Beta-galactoside-specific lectin 3 OS=Viscum album OX=3972 PE=1 SV=2)

HSP 1 Score: 109.4 bits (272), Expect = 6.7e-23
Identity = 80/260 (30.77%), Postives = 117/260 (45.00%), Query Frame = 0

Query: 31  TTHLVGLDGLCLDISPFVTNYISTV-LSPCE-RTKESQLWTIIKDGTIRPKDIRYCLVPQ 90
           T  +VG DGLC+D+     +  + + LSPC+  T  +QLWTI +DGTIR      CL   
Sbjct: 317 TVRIVGRDGLCVDVRDGKFHNGNPIQLSPCKSNTDPNQLWTIRRDGTIRSNG--RCLTTY 376

Query: 91  SNMIGTDAILEECDKGRRSDLNIKWTKMKDGTIRHDESGLVLTGKSNKDAPS-------- 150
               G   ++ +C+   R      W    +GTI +  S LVL   S     +        
Sbjct: 377 GYTAGVYVMIFDCNTAVRE--ATLWQIWGNGTIINPRSNLVLGAASGSSGTTLTVQTQVY 436

Query: 151 ---QSWEATEVFSPTVANIMWLENLCLQSREAVDWHVSLSECNEKDNNQRWAFYGDGTIR 210
              Q W A    +P    I    +LC+++  A  W   +  C     NQ WA YGDG+IR
Sbjct: 437 SLGQGWLAGNDTAPREVTIYGFRDLCMEANGASVW---VETCGSSTENQNWALYGDGSIR 496

Query: 211 QNENRNYCLTSSDRNVGSLV-VVSKCGDKPQQRWSLNAEDKTINHPNTNLVMDATRYIGL 270
             +N++ CLT    +V +++ +VS       QRW    E  TI + N  LVMD  +    
Sbjct: 497 PKQNQDQCLTCQGDSVATVINIVSCSAGSSGQRWVFTNEG-TILNLNNGLVMDVAQ---S 556

Query: 271 SPLPPVIFVDKRDGSSSQQW 277
           +P    I +    G+ +Q W
Sbjct: 557 NPSLRRIIIYPATGNPNQMW 565

BLAST of Tan0018867 vs. NCBI nr
Match: XP_022941585.1 (nigrin b-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 341.7 bits (875), Expect = 6.1e-90
Identity = 174/288 (60.42%), Postives = 210/288 (72.92%), Query Frame = 0

Query: 2   REIVLCIVVAFSLTTHTAMAAAGYGVIGGTTHLVGLDGLCLDISPFVTNYISTVLSPCER 61
           R+++LC+VVAFSL TH   AA GYG  GG THLVG DGLCLD    +  Y  T LS CE+
Sbjct: 5   RQVLLCMVVAFSLATHIG-AALGYGAFGGRTHLVGRDGLCLDTMLLLGYYPPTHLSQCEQ 64

Query: 62  TKESQLWTIIKDGTIRPKDIRYCLVPQS---NMIGTDAILEECDKGRRSDLNIKWTKMKD 121
            K SQLW+I++DGTIR  D+R+CLVP+S     +G + +  +C K  R D+  KWTK KD
Sbjct: 65  RKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDM--KWTKNKD 124

Query: 122 GTIRHDESGLVLTGKS--------NKDAPSQSWEATEVFSPTVANIMWLENLCLQSREAV 181
           GTI HDESGLVLT K         N++ PSQSWEATE F P VANI WL+NLCL+S E  
Sbjct: 125 GTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEGE 184

Query: 182 DWHVSLSECNEKDNNQRWAFYGDGTIRQNENRNYCLTSSDRNVGSLVVVSKCGDKPQQRW 241
           + +V+L+EC+  D NQRWA YGDGTIRQNE R+ CLTSS+RN G++VVVS+C DKPQQRW
Sbjct: 185 NSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKPQQRW 244

Query: 242 SLNAEDKTINHPNTNLVMDATRYIGLSPLPPVIFVDKRDGSSSQQWTI 279
            L AED TINHPNTNLVMD  R + L  LPP+I VDK DG++SQ+WTI
Sbjct: 245 GL-AEDSTINHPNTNLVMDVRREVPL--LPPMIVVDKHDGAASQRWTI 286

BLAST of Tan0018867 vs. NCBI nr
Match: XP_022941584.1 (nigrin b-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 340.9 bits (873), Expect = 1.0e-89
Identity = 174/290 (60.00%), Postives = 211/290 (72.76%), Query Frame = 0

Query: 1   MREIVLCIVVAFSLTTHTAMAAA-GYGVIGGTTHLVGLDGLCLDISPFVTNYISTVLSPC 60
           M +++ CI +AFSL THTA+AAA GYG  G  THLVG DGLCLD    +  Y  T LS C
Sbjct: 4   MTQVLFCIALAFSLATHTAIAAASGYGSFGSRTHLVGRDGLCLDTMLLLGYYPPTHLSQC 63

Query: 61  ERTKESQLWTIIKDGTIRPKDIRYCLVPQS---NMIGTDAILEECDKGRRSDLNIKWTKM 120
           E+ K SQLW+I++DGTIR  D+R+CLVP+S     +G + +  +C K  R D+  KWTK 
Sbjct: 64  EQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDM--KWTKN 123

Query: 121 KDGTIRHDESGLVLTGKS--------NKDAPSQSWEATEVFSPTVANIMWLENLCLQSRE 180
           KDGTI HDESGLVLT K         N++ PSQSWEATE F P VANI WL+NLCL+S E
Sbjct: 124 KDGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIE 183

Query: 181 AVDWHVSLSECNEKDNNQRWAFYGDGTIRQNENRNYCLTSSDRNVGSLVVVSKCGDKPQQ 240
             + +V+L+EC+  D NQRWA YGDGTIRQNE R+ CLTSS+RN G++VVVS+C DKPQQ
Sbjct: 184 GENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKPQQ 243

Query: 241 RWSLNAEDKTINHPNTNLVMDATRYIGLSPLPPVIFVDKRDGSSSQQWTI 279
           RW L AED TINHPNTNLVMD  R + L  LPP+I VDK DG++SQ+WTI
Sbjct: 244 RWGL-AEDSTINHPNTNLVMDVRREVPL--LPPMIVVDKHDGAASQRWTI 288

BLAST of Tan0018867 vs. NCBI nr
Match: XP_022982216.1 (ribosome-inactivating protein SNAI-like [Cucurbita maxima])

HSP 1 Score: 333.2 bits (853), Expect = 2.2e-87
Identity = 173/289 (59.86%), Postives = 207/289 (71.63%), Query Frame = 0

Query: 1   MREIVLCIVVAFSLTTHTAMAAAGYGVIGGTTHLVGLDGLCLDISPFVTNYISTVLSPCE 60
           MR+++LCI +AFSL      A + YG  GG THLVG DGLCLD       Y  T LS C+
Sbjct: 4   MRQVLLCIALAFSL---AIAAPSEYGAFGGRTHLVGRDGLCLDTMLLSGYYPQTDLSQCQ 63

Query: 61  RTKESQLWTIIKDGTIRPKDIRYCLVPQSNMI---GTDAILEECDKGRRSDLNIKWTKMK 120
             K SQLWTI++DGTIR  D+RYCLVP++  I   G + ++ +C K  R D+  KWTK K
Sbjct: 64  PRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEVRHDM--KWTKKK 123

Query: 121 DGTIRHDESGLVLTGK--------SNKDAPSQSWEATEVFSPTVANIMWLENLCLQSREA 180
           DGTI HDESGLVLT K        +N+DAPSQSWEATE   P VANI WL+NLCL+SRE 
Sbjct: 124 DGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLCLKSREG 183

Query: 181 VDWHVSLSECNEKDNNQRWAFYGDGTIRQNENRNYCLTSSDRNVGSLVVVSKCGDKPQQR 240
            + +V+L+EC+  D NQRWA YGDGTIRQNE R+ CLTSS+RN G+LVVVS+C DKPQQR
Sbjct: 184 ENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSRCADKPQQR 243

Query: 241 WSLNAEDKTINHPNTNLVMDATRYIGLSPLPPVIFVDKRDGSSSQQWTI 279
           W L AED TINHPNTNLVMD  R + L  LPP+I VDK DG+ SQ+W+I
Sbjct: 244 WGL-AEDNTINHPNTNLVMDVHREVPL--LPPMIVVDKHDGAPSQRWSI 284

BLAST of Tan0018867 vs. NCBI nr
Match: KAG6600358.1 (hypothetical protein SDJN03_05591, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 332.0 bits (850), Expect = 4.9e-87
Identity = 172/290 (59.31%), Postives = 211/290 (72.76%), Query Frame = 0

Query: 1   MREIVLCIVVAFSLTTHTAMAAA-GYGVIGGTTHLVGLDGLCLDISPFVTNYISTVLSPC 60
           M +++ CI +AFSL THTA+AAA GYG  G  THLVG DGLCL+   F   Y  T LS C
Sbjct: 4   MTQVLFCIALAFSLATHTAIAAASGYGSFGSRTHLVGRDGLCLNTLSFFGYYTPTDLSLC 63

Query: 61  ERTKESQLWTIIKDGTIRPKDIRYCLVPQS---NMIGTDAILEECDKGRRSDLNIKWTKM 120
           E+ K SQLW+I++DGT+R  + R+CLVP+S    ++G + +  +C K  R D+  KWTK 
Sbjct: 64  EQWKSSQLWSILEDGTMRISN-RFCLVPKSQSTEVVGANVVAMDCSKEIRHDM--KWTKN 123

Query: 121 KDGTIRHDESGLVLTGKS--------NKDAPSQSWEATEVFSPTVANIMWLENLCLQSRE 180
           KDGTI HDESGLVLT K         N++ PSQSWEATE F P VANI WL+NLCL+S E
Sbjct: 124 KDGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFYPMVANIKWLDNLCLKSIE 183

Query: 181 AVDWHVSLSECNEKDNNQRWAFYGDGTIRQNENRNYCLTSSDRNVGSLVVVSKCGDKPQQ 240
             + +V+L+EC+  D NQRWA YGDGTIRQNE R+ CLTSS+RN G++VVVS+C DKPQQ
Sbjct: 184 GENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKPQQ 243

Query: 241 RWSLNAEDKTINHPNTNLVMDATRYIGLSPLPPVIFVDKRDGSSSQQWTI 279
           RW L AED TINHPNTNLVMD  R + L  LPP+I VDK DG++SQ+WTI
Sbjct: 244 RWGL-AEDNTINHPNTNLVMDVRREVPL--LPPMIVVDKHDGAASQRWTI 287

BLAST of Tan0018867 vs. NCBI nr
Match: XP_023551012.1 (nigrin b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 332.0 bits (850), Expect = 4.9e-87
Identity = 170/290 (58.62%), Postives = 208/290 (71.72%), Query Frame = 0

Query: 1   MREIVLCIVVAFSLTTHTAMAAA-GYGVIGGTTHLVGLDGLCLDISPFVTNYISTVLSPC 60
           M +++ CI +AFSL THTA+AAA GYG  G  +HLVG DGLCL+       Y  T LS C
Sbjct: 4   MTQVLFCIALAFSLATHTAIAAASGYGSFGARSHLVGRDGLCLNALLLFGYYTPTDLSLC 63

Query: 61  ERTKESQLWTIIKDGTIRPKDIRYCLVPQS---NMIGTDAILEECDKGRRSDLNIKWTKM 120
           E+ K +QLW+I++DGTIR  D+R+CLVP+S     IG + +  +C +  R D+  KWTK 
Sbjct: 64  EQRKSNQLWSILEDGTIRISDVRFCLVPKSQSTEFIGANVVAMDCSREIRHDM--KWTKN 123

Query: 121 KDGTIRHDESGLVLTGKS--------NKDAPSQSWEATEVFSPTVANIMWLENLCLQSRE 180
           KDGTI HDESGLVLT K         N++ PSQSWEATE F+P VANI WL+NLCL+S E
Sbjct: 124 KDGTIHHDESGLVLTAKPGKTVTVEVNENLPSQSWEATETFNPMVANIKWLDNLCLKSIE 183

Query: 181 AVDWHVSLSECNEKDNNQRWAFYGDGTIRQNENRNYCLTSSDRNVGSLVVVSKCGDKPQQ 240
             + +V+L EC+  D NQRWA YGDGTIRQNE R+ CLTS +RN G++VVVS+C DKPQQ
Sbjct: 184 GENSYVTLKECSRDDKNQRWALYGDGTIRQNEYRHLCLTSFERNYGAMVVVSRCADKPQQ 243

Query: 241 RWSLNAEDKTINHPNTNLVMDATRYIGLSPLPPVIFVDKRDGSSSQQWTI 279
           RW L AED TINHPNTNLVMD  R + L  LPP+I V+K DG+ SQQWTI
Sbjct: 244 RWGL-AEDNTINHPNTNLVMDVRREVPL--LPPMIVVEKHDGAPSQQWTI 288

BLAST of Tan0018867 vs. ExPASy TrEMBL
Match: A0A6J1FU34 (nigrin b-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 3.0e-90
Identity = 174/288 (60.42%), Postives = 210/288 (72.92%), Query Frame = 0

Query: 2   REIVLCIVVAFSLTTHTAMAAAGYGVIGGTTHLVGLDGLCLDISPFVTNYISTVLSPCER 61
           R+++LC+VVAFSL TH   AA GYG  GG THLVG DGLCLD    +  Y  T LS CE+
Sbjct: 5   RQVLLCMVVAFSLATHIG-AALGYGAFGGRTHLVGRDGLCLDTMLLLGYYPPTHLSQCEQ 64

Query: 62  TKESQLWTIIKDGTIRPKDIRYCLVPQS---NMIGTDAILEECDKGRRSDLNIKWTKMKD 121
            K SQLW+I++DGTIR  D+R+CLVP+S     +G + +  +C K  R D+  KWTK KD
Sbjct: 65  RKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDM--KWTKNKD 124

Query: 122 GTIRHDESGLVLTGKS--------NKDAPSQSWEATEVFSPTVANIMWLENLCLQSREAV 181
           GTI HDESGLVLT K         N++ PSQSWEATE F P VANI WL+NLCL+S E  
Sbjct: 125 GTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEGE 184

Query: 182 DWHVSLSECNEKDNNQRWAFYGDGTIRQNENRNYCLTSSDRNVGSLVVVSKCGDKPQQRW 241
           + +V+L+EC+  D NQRWA YGDGTIRQNE R+ CLTSS+RN G++VVVS+C DKPQQRW
Sbjct: 185 NSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKPQQRW 244

Query: 242 SLNAEDKTINHPNTNLVMDATRYIGLSPLPPVIFVDKRDGSSSQQWTI 279
            L AED TINHPNTNLVMD  R + L  LPP+I VDK DG++SQ+WTI
Sbjct: 245 GL-AEDSTINHPNTNLVMDVRREVPL--LPPMIVVDKHDGAASQRWTI 286

BLAST of Tan0018867 vs. ExPASy TrEMBL
Match: A0A6J1FNV0 (nigrin b-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=3 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 5.1e-90
Identity = 174/290 (60.00%), Postives = 211/290 (72.76%), Query Frame = 0

Query: 1   MREIVLCIVVAFSLTTHTAMAAA-GYGVIGGTTHLVGLDGLCLDISPFVTNYISTVLSPC 60
           M +++ CI +AFSL THTA+AAA GYG  G  THLVG DGLCLD    +  Y  T LS C
Sbjct: 4   MTQVLFCIALAFSLATHTAIAAASGYGSFGSRTHLVGRDGLCLDTMLLLGYYPPTHLSQC 63

Query: 61  ERTKESQLWTIIKDGTIRPKDIRYCLVPQS---NMIGTDAILEECDKGRRSDLNIKWTKM 120
           E+ K SQLW+I++DGTIR  D+R+CLVP+S     +G + +  +C K  R D+  KWTK 
Sbjct: 64  EQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDM--KWTKN 123

Query: 121 KDGTIRHDESGLVLTGKS--------NKDAPSQSWEATEVFSPTVANIMWLENLCLQSRE 180
           KDGTI HDESGLVLT K         N++ PSQSWEATE F P VANI WL+NLCL+S E
Sbjct: 124 KDGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIE 183

Query: 181 AVDWHVSLSECNEKDNNQRWAFYGDGTIRQNENRNYCLTSSDRNVGSLVVVSKCGDKPQQ 240
             + +V+L+EC+  D NQRWA YGDGTIRQNE R+ CLTSS+RN G++VVVS+C DKPQQ
Sbjct: 184 GENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKPQQ 243

Query: 241 RWSLNAEDKTINHPNTNLVMDATRYIGLSPLPPVIFVDKRDGSSSQQWTI 279
           RW L AED TINHPNTNLVMD  R + L  LPP+I VDK DG++SQ+WTI
Sbjct: 244 RWGL-AEDSTINHPNTNLVMDVRREVPL--LPPMIVVDKHDGAASQRWTI 288

BLAST of Tan0018867 vs. ExPASy TrEMBL
Match: A0A6J1IYQ6 (ribosome-inactivating protein SNAI-like OS=Cucurbita maxima OX=3661 GN=LOC111481109 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 1.1e-87
Identity = 173/289 (59.86%), Postives = 207/289 (71.63%), Query Frame = 0

Query: 1   MREIVLCIVVAFSLTTHTAMAAAGYGVIGGTTHLVGLDGLCLDISPFVTNYISTVLSPCE 60
           MR+++LCI +AFSL      A + YG  GG THLVG DGLCLD       Y  T LS C+
Sbjct: 4   MRQVLLCIALAFSL---AIAAPSEYGAFGGRTHLVGRDGLCLDTMLLSGYYPQTDLSQCQ 63

Query: 61  RTKESQLWTIIKDGTIRPKDIRYCLVPQSNMI---GTDAILEECDKGRRSDLNIKWTKMK 120
             K SQLWTI++DGTIR  D+RYCLVP++  I   G + ++ +C K  R D+  KWTK K
Sbjct: 64  PRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEVRHDM--KWTKKK 123

Query: 121 DGTIRHDESGLVLTGK--------SNKDAPSQSWEATEVFSPTVANIMWLENLCLQSREA 180
           DGTI HDESGLVLT K        +N+DAPSQSWEATE   P VANI WL+NLCL+SRE 
Sbjct: 124 DGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLCLKSREG 183

Query: 181 VDWHVSLSECNEKDNNQRWAFYGDGTIRQNENRNYCLTSSDRNVGSLVVVSKCGDKPQQR 240
            + +V+L+EC+  D NQRWA YGDGTIRQNE R+ CLTSS+RN G+LVVVS+C DKPQQR
Sbjct: 184 ENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSRCADKPQQR 243

Query: 241 WSLNAEDKTINHPNTNLVMDATRYIGLSPLPPVIFVDKRDGSSSQQWTI 279
           W L AED TINHPNTNLVMD  R + L  LPP+I VDK DG+ SQ+W+I
Sbjct: 244 WGL-AEDNTINHPNTNLVMDVHREVPL--LPPMIVVDKHDGAPSQRWSI 284

BLAST of Tan0018867 vs. ExPASy TrEMBL
Match: A0A5A7T854 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001540 PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 2.4e-76
Identity = 159/297 (53.54%), Postives = 204/297 (68.69%), Query Frame = 0

Query: 1   MREIVLCIVVAFSLTTHTAMAAAG-------YGVIGGTTHLVGLDGLCLDISP--FVTNY 60
           M+EIVL I+VAFSLTTH AMA          YG + G THLVG DGLCL++SP  +  N 
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPGATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  ISTVLSPC-ERTKESQLWTIIKDGTIRPKDIRYCLVPQSNMIG--TDAILEECDKGRRSD 120
             T LSPC E  K++QLWT++ DGTIRP + ++CLV    ++G    A++ EC K   S+
Sbjct: 61  FPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADV-LVGVINKAVVRECAK--ESN 120

Query: 121 LNIKWTKMKDGTIRHDESGLVLTG-------KSNKDAPSQSWEATEVFSPTVANIMWLEN 180
            N +WT  KDGTI H ESG+VLTG       +SNK+ PSQSWEATE  +P +ANI WL N
Sbjct: 121 SNKRWTHKKDGTITHVESGMVLTGDSDYVTLQSNKNTPSQSWEATESSTPMIANIEWLNN 180

Query: 181 LCLQSREAVDWHVSLSECNEKDNNQRWAFYGDGTIRQNENRNYCLTSSDRNVGSLVVVSK 240
           LCLQS +  + HV L+ CN ++ +QRWA YGDGTIRQN NRNYCLT SD  +G  +V+SK
Sbjct: 181 LCLQSTDDSN-HVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLT-SDEYLGRFIVMSK 240

Query: 241 CGDKPQQRWSLNAEDKTINHPNTNLVMDATRYIGLSPLPPVIFVDKRDGSSSQQWTI 279
           C DKPQQRW+L A+D TI+HPNT++VMD   ++        +  ++RDGS+SQ+WTI
Sbjct: 241 CEDKPQQRWNLGAKDSTIDHPNTDMVMDVL-FVPYYDFSSAVVTNRRDGSASQRWTI 291

BLAST of Tan0018867 vs. ExPASy TrEMBL
Match: A0A5D3BL95 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00260 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 3.2e-76
Identity = 159/297 (53.54%), Postives = 204/297 (68.69%), Query Frame = 0

Query: 1   MREIVLCIVVAFSLTTHTAMA-------AAGYGVIGGTTHLVGLDGLCLDISP--FVTNY 60
           M+EIVL I+VAFSLTTH AMA          YG + G THLVG DGLCL++SP  +  N 
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPYNYGYGGEYGGVPGATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  ISTVLSPC-ERTKESQLWTIIKDGTIRPKDIRYCLVPQSNMIG--TDAILEECDKGRRSD 120
             T LSPC E  K++QLWT++ DGTIRP + ++CLV    ++G    A++ EC K   S+
Sbjct: 61  FPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADV-LVGVINKAVVRECAK--ESN 120

Query: 121 LNIKWTKMKDGTIRHDESGLVLTG-------KSNKDAPSQSWEATEVFSPTVANIMWLEN 180
            N +WT  KDGTI H ESG+VLTG       +SNK+ PSQSWEATE  +P +ANI WL N
Sbjct: 121 SNKRWTHKKDGTITHVESGMVLTGDSDYVTLQSNKNTPSQSWEATESSTPMIANIEWLNN 180

Query: 181 LCLQSREAVDWHVSLSECNEKDNNQRWAFYGDGTIRQNENRNYCLTSSDRNVGSLVVVSK 240
           LCLQS +  + HV L+ CN ++ +QRWA YGDGTIRQN NRNYCLT SD  +G  +V+SK
Sbjct: 181 LCLQSTDDSN-HVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLT-SDEYLGRFIVMSK 240

Query: 241 CGDKPQQRWSLNAEDKTINHPNTNLVMDATRYIGLSPLPPVIFVDKRDGSSSQQWTI 279
           C DKPQQRW+L A+D TI+HPNT++VMD   ++        +  ++RDGS+SQ+WTI
Sbjct: 241 CEDKPQQRWNLGAKDSTIDHPNTDMVMDVL-FVPYYDFSSAVVTNRRDGSASQRWTI 291