Tan0018673 (gene) Snake gourd v1

BLAST of Tan0018673 vs. ExPASy Swiss-Prot
Match: P17569 (Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 1169.8 bits (3025), Expect = 0.0e+00
Identity = 567/899 (63.07%), Postives = 691/899 (76.86%), Query Frame = 0

Query: 14  PASNSNFPVQRCNSFRSSSFKFKRAAEKSFSVDDDDSSFDR------DDENINYFRDLIR 73
           P   ++ PV+ CN F +S+  +        SV   +++ +       +DEN N FRDLI 
Sbjct: 28  PNHRADSPVRGCN-FPNSNVDYNNNRPLKSSVKIQEAAAEEMEDSCSEDENENEFRDLIV 87

Query: 74  KGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDRLDATRLHHPGPTP- 133
           KG  E+EPSILD RDEGTAD+ I RN  MVRLTGKHPFNSEPPL+RL    +HH   TP 
Sbjct: 88  KGNRELEPSILDHRDEGTADNWIERNASMVRLTGKHPFNSEPPLNRL----MHHGFITPV 147

Query: 134 -------------------------------------LCSQPRSLEFPVTIMCSGNRRKE 193
                                                L ++ R  EFP T++C+GNRRKE
Sbjct: 148 PLHYVRNHGVVPKAKWADWTVEVCGLVKRPAKFTMDQLLNEFRFREFPATLVCAGNRRKE 207

Query: 194 QNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGGDDLPGGGGC 253
           QNM KQ+IGF+WGAA  STS+WR VPL ++LKRCGI SR++GAL +  EG +DLPGGGG 
Sbjct: 208 QNMVKQSIGFNWGAAGVSTSVWRRVPLCDLLKRCGILSRKKGALNVCFEGAEDLPGGGGS 267

Query: 254 SYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWLKRIIVTN 313
            YGTSI++E+AMDP+RDII+AYMQNG+ + PDHGFPVRMI+PGF GGRMVKWLKRIIVT 
Sbjct: 268 KYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVRMIIPGFIGGRMVKWLKRIIVTT 327

Query: 314 RESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVITTPSHDERLPINSQ 373
           +ES+NYYHF DNRVLPSHVD+++A+AE WWYKPE+++ E+NINSVITTP H+E LPIN+ 
Sbjct: 328 KESENYYHFKDNRVLPSHVDADVANAEAWWYKPEHIINELNINSVITTPCHEEILPINAW 387

Query: 374 TTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYWCWIFWSVEV 433
           TTQ  YTLRGY+YSGGGKKVTRV+VT+D GETWQ+C L++ EK NKYGKYWCW FWS+EV
Sbjct: 388 TTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWQVCTLDHPEKANKYGKYWCWCFWSLEV 447

Query: 434 EIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRH 493
           E++DL  +KEIAVRAWDET+NTQPE L WNLMGMMNN W+R+K N+CKP KGEIGIVF H
Sbjct: 448 EVLDLLSAKEIAVRAWDETHNTQPEKLIWNLMGMMNNCWFRVKTNMCKPHKGEIGIVFEH 507

Query: 494 PTLPGNQSGGWKEKDK-------NNPTAAGSPSQPTNNNTTTTSKTFSMSEIKTHNSPES 553
           PT PGNQSGGW ++++       +N T   S S P  N   T S T+++SE+K HNSP+S
Sbjct: 508 PTQPGNQSGGWMDRERHLEISTESNQTLKKSVSTPFMN---TASNTYTLSEVKKHNSPQS 567

Query: 554 AWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGDLLI 613
           AWI+VHGHVYDCT FLK HPGG+DSILINAGTDCTEEFDAIHSDKA KMLEDY+IG+L+ 
Sbjct: 568 AWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDKAKKMLEDYRIGELIT 627

Query: 614 QNDNKEMASSS--------------TPAQD------VALVPREKIPVKLISKTSISHNTR 673
                + +S+S               P ++      VAL P EKIP KLISKTSISH+ R
Sbjct: 628 TGYASDSSSNSPNNSTHGASNFSHLAPIREAPVSRRVALAPNEKIPCKLISKTSISHDVR 687

Query: 674 IFRFALP-SKDQILGLPVGKHIFLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNV 733
           +FRFALP  +DQ LGLPVGKHIF+CATVDGKLCMRAYTP+SS+DE+G+F+LVVK+YFK V
Sbjct: 688 VFRFALPGGQDQALGLPVGKHIFICATVDGKLCMRAYTPTSSIDEMGFFELVVKVYFKGV 747

Query: 734 HPQFPNGGLMSQFLDSLPLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTG 793
           HP+FPNGG+MSQ+LDS+ +GST+DVKGPLGHIEY GRGNF VHGKP+FA++LAM+AGGTG
Sbjct: 748 HPKFPNGGIMSQYLDSMEVGSTLDVKGPLGHIEYTGRGNFMVHGKPRFARRLAMLAGGTG 807

Query: 794 ITPIYQVVQAILKDPADETEMFLVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVG 841
           ITPIYQVVQAILKDP DETEM++VYANRTE+DILLR+ELDTWAK+ +RLK+WYVV++ + 
Sbjct: 808 ITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLRDELDTWAKKNQRLKVWYVVQESIR 867

BLAST of Tan0018673 vs. ExPASy Swiss-Prot
Match: P39870 (Inducible nitrate reductase [NADH] 2 OS=Glycine max OX=3847 GN=INR2 PE=2 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 558/852 (65.49%), Postives = 661/852 (77.58%), Query Frame = 0

Query: 49  DSSFDRDDENINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSE 108
           DSS D +++     +DLIRKG  E+E SI DPRD+GT+D  I RN  +VRLTGKHPFNSE
Sbjct: 42  DSSSDEEEDTTLNLKDLIRKGTTEVESSIFDPRDDGTSDHWIQRNSSLVRLTGKHPFNSE 101

Query: 109 PPLDR-----------LDATRLHHPGP-----------TPLCSQPR------------SL 168
           PPL R           L   R H P P           T L ++P             S 
Sbjct: 102 PPLPRLMHHGFITPVPLHYVRNHGPVPRARWEDWTVEVTGLVTRPTCFTMEQLLHDFPSR 161

Query: 169 EFPVTIMCSGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALY 228
           EFP T++C+GNRRKEQNM KQ+IGF+WGAA  STS+WRGVPLR +LK CGI++R +GAL+
Sbjct: 162 EFPATLVCAGNRRKEQNMVKQSIGFNWGAAAISTSVWRGVPLRTLLKSCGIYTRTKGALH 221

Query: 229 IWMEGGDDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFT 288
           +  EG +DLPGGGG  YGTSI RE+A+DPSRDII+AYMQNG+P+ PDHGFPVRMI+PGF 
Sbjct: 222 VCFEGAEDLPGGGGSKYGTSILREVALDPSRDIILAYMQNGEPLSPDHGFPVRMIIPGFI 281

Query: 289 GGRMVKWLKRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSV 348
           GGRMVKWLKRIIVT  +S NYYH+ DNRVLPSHVD+ELA+A+ WWYKP+Y++ E+NINSV
Sbjct: 282 GGRMVKWLKRIIVTTDQSQNYYHYKDNRVLPSHVDAELANAQAWWYKPDYIINELNINSV 341

Query: 349 ITTPSHDERLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPN 408
           ITTP H+E LPINS TTQ  Y +RGYAYSGGG+KVTRV+VTLDGGETWQ+C L+  EKPN
Sbjct: 342 ITTPCHEEILPINSWTTQMPYFIRGYAYSGGGRKVTRVEVTLDGGETWQVCTLDCPEKPN 401

Query: 409 KYGKYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKIN 468
           KYGKYWCW  WSVEVE++DL G++EIAVRAWDE  NTQPE L WN+MGMMNN W+R+K N
Sbjct: 402 KYGKYWCWCLWSVEVEVLDLLGAREIAVRAWDEALNTQPEKLIWNVMGMMNNCWFRVKTN 461

Query: 469 ICKPQKGEIGIVFRHPTLPGNQSGGWKEKDK-------NNPTAAGSPSQPTNNNTTTTSK 528
           +C+P KGEIGIVF HPT PGNQSGGW  K+K       +NPT   S S P  N   TTSK
Sbjct: 462 VCRPHKGEIGIVFEHPTQPGNQSGGWMAKEKHLEKSSESNPTLKKSVSSPFMN---TTSK 521

Query: 529 TFSMSEIKTHNSPESAWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDK 588
           T++MSE++ HN+ +SAWI+VHGHVYDCT FLK HPGG DSILINAGTDCTEEF+AIHSDK
Sbjct: 522 TYTMSEVRRHNNADSAWIIVHGHVYDCTRFLKDHPGGTDSILINAGTDCTEEFEAIHSDK 581

Query: 589 AIKMLEDYKIGDLLIQNDNKEMASSS-------------------TPAQDVALVPREKIP 648
           A +MLEDY+IG+L     N + +SS+                   TP + VAL PREKIP
Sbjct: 582 AKQMLEDYRIGELTTTCYNSDSSSSNPSVHGSSDTIPLTPIKEVITPMRSVALNPREKIP 641

Query: 649 VKLISKTSISHNTRIFRFALPSKDQILGLPVGKHIFLCATVDGKLCMRAYTPSSSVDEVG 708
            KLISKTSISH+ R+FRFALPS D ++GLPVGKHIFLCATVD KLCMRAYTP+SSV EVG
Sbjct: 642 CKLISKTSISHDVRLFRFALPSDDLLMGLPVGKHIFLCATVDEKLCMRAYTPTSSVHEVG 701

Query: 709 YFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPLGSTVDVKGPLGHIEYMGRGNFTVHGKPK 768
           YFDLVVK+YFK VHP+FP GG+MSQ LDSLP+GS +DVKGPLGHI Y GRGNF VHGKP+
Sbjct: 702 YFDLVVKVYFKGVHPKFPTGGIMSQHLDSLPIGSVLDVKGPLGHIVYTGRGNFLVHGKPR 761

Query: 769 FAKKLAMVAGGTGITPIYQVVQAILKDPADETEMFLVYANRTEEDILLREELDTWAKETE 828
           FA +LAM+AGGTGITPIYQVV+AILKDP D TEM +VYANRTE+DILL+EELD WAK+ +
Sbjct: 762 FATRLAMLAGGTGITPIYQVVRAILKDPEDCTEMHVVYANRTEDDILLKEELDEWAKKYD 821

Query: 829 RLKIWYVVKDGVGEGWPYSVGYITEGVLREHVPMGSPETLALVCGPPPMIELTVKPILKS 841
           RLK+WYV++  + EGW YSVG+ITE +L EH+P  SP+TLAL CGPPPMI+  V+P L+ 
Sbjct: 822 RLKVWYVIQASIREGWEYSVGFITESILTEHIPNASPDTLALTCGPPPMIQFAVQPNLEK 881

BLAST of Tan0018673 vs. ExPASy Swiss-Prot
Match: P27783 (Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1)

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 573/906 (63.25%), Postives = 688/906 (75.94%), Query Frame = 0

Query: 1   MAAAVDHKTL-DLEPASNSNF----PVQRCNSFRSSSFKFKR----AAEKSFSVDDDDSS 60
           MAA+V+++     EPA N       PV   NS RS S    R    + +K  +  +D SS
Sbjct: 1   MAASVENRRFTHHEPAVNGLVRTFKPVP--NSHRSDSPDLGRQIPSSPKKQVATGEDSSS 60

Query: 61  FDRDDENINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPL 120
              +DEN N +++LI+KG  E+EPSILDPRDE TAD+ + RN  MVRLTGKHPFNSE PL
Sbjct: 61  ---EDENENDYKELIQKGNGELEPSILDPRDEATADNWVERNATMVRLTGKHPFNSEAPL 120

Query: 121 DRL------DATRLHH---PGPTP-------------------------LCSQPRSLEFP 180
            RL          LH+    GP P                         L ++ RS EFP
Sbjct: 121 TRLMHHGFITPAPLHYVRNHGPVPKARWEDWSVEVCGLVKRPARFTMDRLVTEFRSREFP 180

Query: 181 VTIMCSGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWM 240
           VT++C+GNRRKEQNM K+TIGF+WGAA  STS+WRGVPLR+VLKRCGIFSR RGA  +  
Sbjct: 181 VTLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFSRGRGAFNVCF 240

Query: 241 EGGDDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGR 300
           EG +DLPGGGG  YGTS++ EMAMDP+RDII+ YMQNG+ + PDHGFPVRMI+PGF GGR
Sbjct: 241 EGAEDLPGGGGSKYGTSVKYEMAMDPARDIILGYMQNGERLSPDHGFPVRMIIPGFIGGR 300

Query: 301 MVKWLKRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVITT 360
           MVKWLKRIIVT +ESDNYYH+ DNRVLPSHVD+++A AE WWYKPE+++ E+NINSVITT
Sbjct: 301 MVKWLKRIIVTTKESDNYYHYNDNRVLPSHVDADVAKAEAWWYKPEHIINELNINSVITT 360

Query: 361 PSHDERLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYG 420
           P H+E LPINS TTQ  YTLRGYAYSGGG+KVTRV++T++GGE W++C L++ EKPNKYG
Sbjct: 361 PCHEEILPINSWTTQRPYTLRGYAYSGGGRKVTRVEITMNGGEKWRVCALDHPEKPNKYG 420

Query: 421 KYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICK 480
           KYWCW FWS+EVE++DL G+KEIAVRAWDE +NTQPE L WN+MGMMNN W+R+K N+CK
Sbjct: 421 KYWCWCFWSLEVEVLDLLGAKEIAVRAWDEAHNTQPEKLIWNVMGMMNNCWFRVKTNVCK 480

Query: 481 PQKGEIGIVFRHPTLPGNQSGGWKEKDKN-------NPTAAGSPSQPTNNNTTTTSKTFS 540
              GEIGI F HPT+PGN+SGGW  ++KN       N +   S S P  N   T+SK FS
Sbjct: 481 AHMGEIGIAFEHPTVPGNESGGWMAREKNLETSSDANQSLKKSVSSPFMN---TSSKMFS 540

Query: 541 MSEIKTHNSPESAWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIK 600
           MSE+K HNS ESAWI+VHGH+YDCT FLK HPGGADSILINAGTDCTEEFDAIHSDKA K
Sbjct: 541 MSEVKKHNSAESAWIIVHGHIYDCTHFLKDHPGGADSILINAGTDCTEEFDAIHSDKAKK 600

Query: 601 MLEDYKIGDLLI-------QNDNKEMASSST---------PAQDVALVPREKIPVKLISK 660
           MLEDY+IG+L+         N     AS+++         P ++VAL+P  KIP KL+ K
Sbjct: 601 MLEDYRIGELITTGYVSDSPNSTVHGASNTSHLAPIKEIAPLRNVALIPGAKIPTKLVYK 660

Query: 661 TSISHNTRIFRFALPSKDQILGLPVGKHIFLCATVDGKLCMRAYTPSSSVDEVGYFDLVV 720
            S+SH+ R+FR ALPS DQ+LGLPVGKH+FLCAT+D KLCMRAYTP+S++DEVGY DLVV
Sbjct: 661 KSLSHDVRLFRLALPSDDQVLGLPVGKHVFLCATIDDKLCMRAYTPTSTIDEVGYLDLVV 720

Query: 721 KIYFKNVHPQFPNGGLMSQFLDSLPLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLA 780
           KIYFKN +P+FPNGGLMSQ LDSLP+GS + VKGPLGH+EY GRGNF VHG+PKFAK+LA
Sbjct: 721 KIYFKNSNPRFPNGGLMSQHLDSLPIGSVLHVKGPLGHVEYTGRGNFLVHGEPKFAKRLA 780

Query: 781 MVAGGTGITPIYQVVQAILKDPADETEMFLVYANRTEEDILLREELDTWAKETERLKIWY 840
           MVAGGTGITPIYQV+QAILKDP DETEMF+VYANRTE+DILLREELD WAK+ E+LK+WY
Sbjct: 781 MVAGGTGITPIYQVIQAILKDPEDETEMFVVYANRTEDDILLREELDDWAKKHEKLKVWY 840

BLAST of Tan0018673 vs. ExPASy Swiss-Prot
Match: P08509 (Nitrate reductase [NADH] 2 OS=Nicotiana tabacum OX=4097 GN=NIA2 PE=2 SV=2)

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 563/904 (62.28%), Postives = 685/904 (75.77%), Query Frame = 0

Query: 1   MAAAVDHKTLD-LEPASNSNF------PVQRCNSFRSSSFKFKRAAEKSFSVDDDDSSFD 60
           MAA+V+++    LE   + +F      PV+ CN    +S  F++    +  +D   S  D
Sbjct: 1   MAASVENRQFSHLEAGLSRSFKPRSDSPVRGCNFPSPNSTNFQKKPNSTIYLDYSSSEDD 60

Query: 61  RDDENINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDR 120
            DD+  N +  +I+KG  E+EPS+ D RDEGTAD+ I RN  M+RLTGKHPFNSEPPL+R
Sbjct: 61  DDDDEKNEYLQMIKKGNSELEPSVHDTRDEGTADNWIERNFSMIRLTGKHPFNSEPPLNR 120

Query: 121 -----------LDATRLHHPGP-----------TPLCSQPRSL------------EFPVT 180
                      L   R H P P           T L  +P               E PVT
Sbjct: 121 LMHHGFITPVPLHYVRNHGPVPKGTWDDWTVEVTGLVKRPMKFTMDQLVNEFPCRELPVT 180

Query: 181 IMCSGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEG 240
           ++C+GNRRKEQNM KQTIGF+WGAA  ST++WRGVPLR +LKRCG+FS+ +GAL +  EG
Sbjct: 181 LVCAGNRRKEQNMVKQTIGFNWGAAAVSTTIWRGVPLRALLKRCGVFSKNKGALNVCFEG 240

Query: 241 GDDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMV 300
            D LPGGGG  YGTSI++E AMDP+RDII+AYMQNG+ + PDHGFPVRMI+PGF GGRMV
Sbjct: 241 ADVLPGGGGSKYGTSIKKEFAMDPARDIIVAYMQNGEKLAPDHGFPVRMIIPGFIGGRMV 300

Query: 301 KWLKRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVITTPS 360
           KW+KRIIVT +ESD+YYHF DNRVLP HVD+ELA+ E WWYKPEY++ E+NINSVITTP 
Sbjct: 301 KWIKRIIVTTQESDSYYHFKDNRVLPPHVDAELANTEAWWYKPEYIINELNINSVITTPC 360

Query: 361 HDERLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKY 420
           H+E LPIN+ TTQ  YTLRGY+YSGGGKKVTRV+VTLDGGETWQ+  L++ EKP KYGKY
Sbjct: 361 HEEILPINAWTTQRPYTLRGYSYSGGGKKVTRVEVTLDGGETWQVSTLDHPEKPTKYGKY 420

Query: 421 WCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQ 480
           WCW FWS+EVE++DL  +KEIAVRAWDET NTQPE L WN+MGMMNN W+R+K+N+CKP 
Sbjct: 421 WCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKLIWNVMGMMNNCWFRVKMNVCKPH 480

Query: 481 KGEIGIVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNNNTT----TTSKTFSMSEIK 540
           KGEIGIVF HPT PGNQSGGW  K+++   +A +P     + +T    T SK +SMSE++
Sbjct: 481 KGEIGIVFEHPTQPGNQSGGWMAKERHLEISAEAPQTLKKSISTPFMNTASKMYSMSEVR 540

Query: 541 THNSPESAWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDY 600
            H+S +SAWI+VHGH+YD T FLK HPGG DSILINAGTDCTEEFDAIHSDKA K+LED+
Sbjct: 541 KHSSADSAWIIVHGHIYDATRFLKDHPGGTDSILINAGTDCTEEFDAIHSDKAKKLLEDF 600

Query: 601 KIGDLLIQN------DNKEMASSS-----------TPAQ-DVALVPREKIPVKLISKTSI 660
           +IG+L+          N    SSS            PAQ  VAL+PREKIP KLI K SI
Sbjct: 601 RIGELITTGYTSDSPGNSVHGSSSFSSFLAPIKELVPAQRSVALIPREKIPCKLIDKQSI 660

Query: 661 SHNTRIFRFALPSKDQILGLPVGKHIFLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIY 720
           SH+ R FRFALPS+DQ+LGLPVGKHIFLCA +D KLCMRAYTP+S++DEVGYF+LVVKIY
Sbjct: 661 SHDVRKFRFALPSEDQVLGLPVGKHIFLCAVIDDKLCMRAYTPTSTIDEVGYFELVVKIY 720

Query: 721 FKNVHPQFPNGGLMSQFLDSLPLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVA 780
           FK +HP+FPNGG MSQ+LDS+PLGS +DVKGPLGHIEY G+GNF VHGK KFAKKLAM+A
Sbjct: 721 FKGIHPKFPNGGQMSQYLDSMPLGSFLDVKGPLGHIEYQGKGNFLVHGKQKFAKKLAMIA 780

Query: 781 GGTGITPIYQVVQAILKDPADETEMFLVYANRTEEDILLREELDTWA-KETERLKIWYVV 840
           GGTGITP+YQV+QAILKDP D+TEM++VYANRTE+DILL+EELD+WA K  ER+K+WYVV
Sbjct: 781 GGTGITPVYQVMQAILKDPEDDTEMYVVYANRTEDDILLKEELDSWAEKIPERVKVWYVV 840

BLAST of Tan0018673 vs. ExPASy Swiss-Prot
Match: P36859 (Nitrate reductase [NADH] OS=Petunia hybrida OX=4102 GN=NIA PE=2 SV=1)

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 572/909 (62.93%), Postives = 685/909 (75.36%), Query Frame = 0

Query: 1   MAAAVDHKTLD-LEPA---------SNSNFPVQRCN-SFRSSSFKFKRAAEKSFSVDDDD 60
           MAA+V+++    LEP            S+ PV+ CN    +    F++    +  +D   
Sbjct: 1   MAASVENRQFSHLEPGLSGVVRSFKPRSDSPVRGCNFPLNNELTNFQKKPNTTIYLDCSS 60

Query: 61  SSFDRDDENINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEP 120
           S  D DD++ N +  +IRKGK E+EPS+ D RDEGTAD+ I RN  M+RLTGKHPFNSEP
Sbjct: 61  SEDDDDDDDKNEYLQMIRKGKLEVEPSVHDIRDEGTADNWIERNNSMIRLTGKHPFNSEP 120

Query: 121 PLDR-----------LDATRLHHPGP-----------TPLCSQPR------------SLE 180
           PL R           L   R H P P           T L  +P             S E
Sbjct: 121 PLARLMHHGFITPVPLHYVRNHGPVPKGMWDDWTVEVTGLVKRPMKFTMEQLVNEFPSRE 180

Query: 181 FPVTIMCSGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYI 240
            PVT++C+GNRRKEQNM KQTIGF+WGAA  ST++WRGVPLR +LKRCGI+SR +GAL I
Sbjct: 181 LPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTVWRGVPLRAILKRCGIYSRTKGALNI 240

Query: 241 WMEGGDDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTG 300
             EG D LPGGGG  YGTSI++E AMDPSRDIIIAYMQNG+ + PDHGFP+RMI+PGF G
Sbjct: 241 CFEGADVLPGGGGSKYGTSIKKEFAMDPSRDIIIAYMQNGEKLTPDHGFPLRMIIPGFIG 300

Query: 301 GRMVKWLKRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVI 360
           GRMVKWLKRIIVT +ES++YYH+ DNRVLP HVD+ELA+AE WWYKPEY++ E+NINSVI
Sbjct: 301 GRMVKWLKRIIVTTQESESYYHYKDNRVLPPHVDAELANAEAWWYKPEYIINELNINSVI 360

Query: 361 TTPSHDERLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNK 420
           TTP H+E LPINS TTQ  YTLRGY+YSGGGKKVTRV+VT+DGGETW +C +++ EKPNK
Sbjct: 361 TTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWNVCTVDHPEKPNK 420

Query: 421 YGKYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINI 480
           YGKYWCW FWS+EVE++DL  +KEIAVRAWDET NTQPE L WN+MGMMNN W+R+K N+
Sbjct: 421 YGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKLIWNVMGMMNNCWFRVKTNV 480

Query: 481 CKPQKGEIGIVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNNNTT----TTSKTFSM 540
           CKP KGEIGIVF HPT PGN SGGW  K+++   +A +P     + +T    T SK +SM
Sbjct: 481 CKPHKGEIGIVFEHPTQPGNLSGGWMAKERHLEISAEAPPTLKKSISTPFMNTASKMYSM 540

Query: 541 SEIKTHNSPESAWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKM 600
           SE+K HNS +SAWI+VHGHVYD T FLK HPGG DSILINAGTDCTEEFDAIHSDKA K+
Sbjct: 541 SEVKKHNSADSAWIIVHGHVYDATRFLKDHPGGIDSILINAGTDCTEEFDAIHSDKAKKL 600

Query: 601 LEDYKIGDLLIQN-------DNKEMASSS-----------TPA-QDVALVPREKIPVKLI 660
           LED++IG+L+          +N    SSS            PA + VAL+PREKIP KL+
Sbjct: 601 LEDFRIGELITTGYTSDSSPNNSVHGSSSFSGFLAPIKELAPAVRSVALIPREKIPCKLV 660

Query: 661 SKTSISHNTRIFRFALPSKDQILGLPVGKHIFLCATVDGKLCMRAYTPSSSVDEVGYFDL 720
            K SISH+ R FRFALPS+DQ+LGLPVGKHIFLCA +D KLCMRAYTP+S+VDEVGYF+L
Sbjct: 661 DKKSISHDVRKFRFALPSEDQVLGLPVGKHIFLCAIIDDKLCMRAYTPTSTVDEVGYFEL 720

Query: 721 VVKIYFKNVHPQFPNGGLMSQFLDSLPLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKK 780
           VVKIYFK + P+FPNGG MSQ+LDSLPLG+ VDVKGPLGHIEY GRGNF VHGK KFAKK
Sbjct: 721 VVKIYFKGIVPKFPNGGQMSQYLDSLPLGAFVDVKGPLGHIEYQGRGNFLVHGKRKFAKK 780

Query: 781 LAMVAGGTGITPIYQVVQAILKDPADETEMFLVYANRTEEDILLREELDTWA-KETERLK 840
           LAM+AGGTGITP+YQV+QAILKDP DETEM +VYANRTE+DILL++ELD+WA K  ER+K
Sbjct: 781 LAMLAGGTGITPVYQVMQAILKDPEDETEMHVVYANRTEDDILLKDELDSWAVKLPERVK 840

BLAST of Tan0018673 vs. NCBI nr
Match: XP_038875756.1 (nitrate reductase [NADH]-like [Benincasa hispida])

HSP 1 Score: 1530.4 bits (3961), Expect = 0.0e+00
Identity = 754/877 (85.97%), Postives = 784/877 (89.40%), Query Frame = 0

Query: 1   MAAAVDHKTLDLEPASNSNFPVQRCNSFRSSSFKFKRAAEKSFSVDDDDSSFDRDDENIN 60
           MAA V+ K+LDLEP SN NFPVQRCNSFRSSSFK KR AEK   VDDDDSSFDRDDENI+
Sbjct: 1   MAATVNPKSLDLEPVSNGNFPVQRCNSFRSSSFKLKRHAEKPLIVDDDDSSFDRDDENIH 60

Query: 61  YFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDR------- 120
           YFRDLIRKGKEEIE SILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPL+R       
Sbjct: 61  YFRDLIRKGKEEIEASILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLERLMRHGFI 120

Query: 121 ----LDATRLHHPGP-----------TPLCSQP------------RSLEFPVTIMCSGNR 180
               L   R H P P           T L   P            RSLEFPVTIMCSGNR
Sbjct: 121 TPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLLNDFRSLEFPVTIMCSGNR 180

Query: 181 RKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGGDDLPGG 240
           RKEQNM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEG DDLPGG
Sbjct: 181 RKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGADDLPGG 240

Query: 241 GGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWLKRII 300
           GGCSYGTSIRREMAMDPSRDIIIAY QNGDP+PPDHGFPVRMIVPGFTGGRMVKWLKRII
Sbjct: 241 GGCSYGTSIRREMAMDPSRDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRII 300

Query: 301 VTNRESDNYYHFMDNRVLPSHV-DSELAHAEGWWYKPEYVVYEMNINSVITTPSHDERLP 360
           VTNRESDNYYHFMDNRVLPSHV DSELAHAEGWWYKPEYV+YEMNINSVITTPSHDE LP
Sbjct: 301 VTNRESDNYYHFMDNRVLPSHVADSELAHAEGWWYKPEYVIYEMNINSVITTPSHDETLP 360

Query: 361 INSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYWCWIFW 420
           INSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETW+ICNLEY+EKPNKYGKYWCWIFW
Sbjct: 361 INSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYYEKPNKYGKYWCWIFW 420

Query: 421 SVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIGI 480
           S+EVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIGI
Sbjct: 421 SIEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIGI 480

Query: 481 VFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNN-NTTTTSKTFSMSEIKTHNSPESAW 540
           VFRHPTL GNQSGGWKEKDK  PT     SQP+NN  TTTT+ TFSMSE+KTHNS ESAW
Sbjct: 481 VFRHPTLAGNQSGGWKEKDKPIPT--NYSSQPSNNKTTTTTTTTFSMSEVKTHNSLESAW 540

Query: 541 IVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGDLLIQN 600
           IVVH HVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKML+DYKIGDLLI N
Sbjct: 541 IVVHNHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLQDYKIGDLLILN 600

Query: 601 DNKEMASSSTP-AQDVALVPREKIPVKLISKTSISHNTRIFRFALPSKDQILGLPVGKHI 660
           DNK+  SS  P  + VALVPREKIPVKLISK SISHNTRIFRF+L S+DQ LGLPVGKHI
Sbjct: 601 DNKDDTSSPKPITKHVALVPREKIPVKLISKISISHNTRIFRFSLSSEDQTLGLPVGKHI 660

Query: 661 FLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPLGST 720
           FLCA +DGKLCMRAYTPSS+VD++GYFDLVVKIYFKN+HP FPNGGLMSQ+LDSLPL S 
Sbjct: 661 FLCANIDGKLCMRAYTPSSAVDQMGYFDLVVKIYFKNIHPDFPNGGLMSQYLDSLPLSSI 720

Query: 721 VDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADETEMF 780
           VD+KGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP DETEMF
Sbjct: 721 VDIKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMF 780

Query: 781 LVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLREHVPMG 840
           +VYANRTEEDILLREELDTWAKE +RLKIWYVVK+GV EGWPYSVGYI E VLREH+PMG
Sbjct: 781 VVYANRTEEDILLREELDTWAKENQRLKIWYVVKEGVKEGWPYSVGYIRECVLREHIPMG 840

BLAST of Tan0018673 vs. NCBI nr
Match: XP_023551317.1 (nitrate reductase [NADH]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1501.1 bits (3885), Expect = 0.0e+00
Identity = 731/880 (83.07%), Postives = 779/880 (88.52%), Query Frame = 0

Query: 1   MAAAVDHKTLDLEPASNSNFPVQRCNSFRSSSFKFKRAAEKSFSV----DDDDSSFDRDD 60
           MAA VDHKTLD+EP +N NF  QRCNSFRS SFK KR  E + ++    DDDDSSFDR D
Sbjct: 1   MAATVDHKTLDMEPPTNGNFLAQRCNSFRSPSFKIKRHPEITLTINDDDDDDDSSFDRHD 60

Query: 61  ENINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDRL-- 120
           +N +YFRDLIRKGKEEIEPS+LDPRDEGTAD+GI+RNP M+RLTGKHPFNSEPPLDRL  
Sbjct: 61  DNNDYFRDLIRKGKEEIEPSVLDPRDEGTADNGIIRNPSMIRLTGKHPFNSEPPLDRLMR 120

Query: 121 ----DATRLHH---PGPTP-------------------------LCSQPRSLEFPVTIMC 180
                   LH+    GP P                         L +  RS+EFPVTI C
Sbjct: 121 HGFITPAPLHYVRNHGPVPKADSSNWTIEIIGLLENPTTFTLQQLVNDFRSIEFPVTITC 180

Query: 181 SGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGGDD 240
           SGNRRKEQNM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSR RGALY+WMEG DD
Sbjct: 181 SGNRRKEQNMFKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRHRGALYVWMEGADD 240

Query: 241 LPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWL 300
           LPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWL
Sbjct: 241 LPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWL 300

Query: 301 KRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVITTPSHDE 360
           KRIIVTNRESDN+YHFMDNRVLPSHVDSELA+AEGWWYKPEYV+YEMNINSVITTPSHDE
Sbjct: 301 KRIIVTNRESDNHYHFMDNRVLPSHVDSELANAEGWWYKPEYVIYEMNINSVITTPSHDE 360

Query: 361 RLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYWCW 420
            L INSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETW+ICNLEYHEKPNKYGKYWCW
Sbjct: 361 MLSINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCW 420

Query: 421 IFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGE 480
           IFWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKPQ GE
Sbjct: 421 IFWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKPQNGE 480

Query: 481 IGIVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNN-NTTTTSKTFSMSEIKTHNSPE 540
           +GIVFRHPTLPGNQSGGWKEKDK NPT   S S+PTN+ N TT +KTF MSE+KTHN+PE
Sbjct: 481 LGIVFRHPTLPGNQSGGWKEKDKINPTDPPS-SKPTNDKNKTTAAKTFPMSEVKTHNTPE 540

Query: 541 SAWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGDLL 600
           S+WIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAI MLEDY IGDLL
Sbjct: 541 SSWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLEDYMIGDLL 600

Query: 601 IQNDNKEMASSSTPA-QDVALVPREKIPVKLISKTSISHNTRIFRFALPSKDQILGLPVG 660
            +ND++E  SSS    Q VALVP +KIPVKLISK SISHNTRIFRF L SKDQ+LGLPVG
Sbjct: 601 TENDSEEKTSSSEAVEQHVALVPGKKIPVKLISKISISHNTRIFRFGLSSKDQVLGLPVG 660

Query: 661 KHIFLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPL 720
           KHIFLCA ++GKLCMRAYTPSSSVDE+G+FDLVVK+YFKN+HP FPNGGLMSQ+LDSLPL
Sbjct: 661 KHIFLCANIEGKLCMRAYTPSSSVDEMGFFDLVVKVYFKNMHPDFPNGGLMSQYLDSLPL 720

Query: 721 GSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADET 780
           GS VDVKGPLGHIEY+GRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP DET
Sbjct: 721 GSIVDVKGPLGHIEYLGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDET 780

Query: 781 EMFLVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLREHV 840
           EM++VYANRTEEDILLREELDTWAKE ERLKIWYVVKDGV EGWPYSVGYI E VLREH+
Sbjct: 781 EMYVVYANRTEEDILLREELDTWAKENERLKIWYVVKDGVKEGWPYSVGYIKESVLREHI 840

BLAST of Tan0018673 vs. NCBI nr
Match: KAG6578997.1 (hypothetical protein SDJN03_23445, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1497.3 bits (3875), Expect = 0.0e+00
Identity = 731/882 (82.88%), Postives = 778/882 (88.21%), Query Frame = 0

Query: 1   MAAAVDHKTLDLEPASNSNFPVQRCNSFRSSSFKFKRAAEKSFSV------DDDDSSFDR 60
           MAA VDHKTLD+EP +N +F  QRCNSFRS SFK KR  E + ++      DDDDSSFDR
Sbjct: 1   MAAVVDHKTLDMEPPTNGSFLAQRCNSFRSPSFKIKRQPEITLTIDDDDDDDDDDSSFDR 60

Query: 61  DDENINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDR- 120
            D+N +YFRDLIRKGKEEIEPSILDPRDEGTAD+GI+RNP M+RLTGKHPFNSEPPLDR 
Sbjct: 61  HDDNNDYFRDLIRKGKEEIEPSILDPRDEGTADNGILRNPSMIRLTGKHPFNSEPPLDRL 120

Query: 121 ----------LDATRLHHPGP-----------TPLCSQP------------RSLEFPVTI 180
                     L   R H P P           T L   P            RS+EFPVTI
Sbjct: 121 MRHGFITPAPLHYVRNHGPVPKADSSNWTIEITGLLENPTTFTLHQLVNDFRSIEFPVTI 180

Query: 181 MCSGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGG 240
            CSGNRRKEQNM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSR RGALY+WMEG 
Sbjct: 181 TCSGNRRKEQNMFKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRHRGALYVWMEGA 240

Query: 241 DDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVK 300
           DDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVR+IVPGFTGGRMVK
Sbjct: 241 DDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRLIVPGFTGGRMVK 300

Query: 301 WLKRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVITTPSH 360
           WLKRIIVTNRESDN+YHFMDNRVLPSHVDSELA+AEGWWYKPEYV+YEMNINSVITTPSH
Sbjct: 301 WLKRIIVTNRESDNHYHFMDNRVLPSHVDSELANAEGWWYKPEYVIYEMNINSVITTPSH 360

Query: 361 DERLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYW 420
           DE L I SQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETW+ICNLEYHEKPNKYGKYW
Sbjct: 361 DEMLSIKSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYW 420

Query: 421 CWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQK 480
           CWIFWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKPQ 
Sbjct: 421 CWIFWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKPQN 480

Query: 481 GEIGIVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNN-NTTTTSKTFSMSEIKTHNS 540
           GE+GIVFRHPTLPGNQSGGWKEKDK NPT   S S+PTN+ N TT SKTF+MSE+KTHN+
Sbjct: 481 GELGIVFRHPTLPGNQSGGWKEKDKINPTDPSS-SKPTNDKNKTTASKTFAMSEVKTHNT 540

Query: 541 PESAWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGD 600
           PES+WIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAI ML+DY IGD
Sbjct: 541 PESSWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLQDYIIGD 600

Query: 601 LLIQNDNKEMASSSTPA-QDVALVPREKIPVKLISKTSISHNTRIFRFALPSKDQILGLP 660
           LL +NDN+E  SSS P  Q VALVP +KIPVKLISK SIS NTRIFRF L SKDQ+LGLP
Sbjct: 601 LLTENDNEENTSSSEPVEQHVALVPGKKIPVKLISKISISQNTRIFRFGLSSKDQVLGLP 660

Query: 661 VGKHIFLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSL 720
           VGKHIFLCA ++GKLCMRAYTPSSSVDE+G+FDLVVK+YFKNVHP FPNGGLMSQ+LDSL
Sbjct: 661 VGKHIFLCAIIEGKLCMRAYTPSSSVDEMGFFDLVVKVYFKNVHPDFPNGGLMSQYLDSL 720

Query: 721 PLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPAD 780
           PLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP D
Sbjct: 721 PLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPED 780

Query: 781 ETEMFLVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLRE 840
           +TEM++VYANRT EDILLREELDTWAKE ERLKIWYVVK+GV EGWPYSVGYI E VLRE
Sbjct: 781 KTEMYVVYANRTVEDILLREELDTWAKENERLKIWYVVKEGVKEGWPYSVGYIKESVLRE 840

BLAST of Tan0018673 vs. NCBI nr
Match: XP_022938867.1 (nitrate reductase [NADH]-like [Cucurbita moschata])

HSP 1 Score: 1495.7 bits (3871), Expect = 0.0e+00
Identity = 732/880 (83.18%), Postives = 777/880 (88.30%), Query Frame = 0

Query: 1   MAAAVDHKTLDLEPASNSNFPVQRCNSFRSSSFKFKRAAEKSFSV----DDDDSSFDRDD 60
           MAA VDHKTLD+EP +N +F  QRCNSFRS SFK KR  E + ++    DDDDSSFDR D
Sbjct: 1   MAAVVDHKTLDMEPPTNGSFLAQRCNSFRSPSFKIKRQPEITLTIDDDDDDDDSSFDRHD 60

Query: 61  ENINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDR--- 120
           +N +YFRDLIRKGKEEIE SILDPRDEGTAD+GI+RNP M+RLTGKHPFNSEPPLDR   
Sbjct: 61  DNNDYFRDLIRKGKEEIELSILDPRDEGTADNGILRNPSMIRLTGKHPFNSEPPLDRLMR 120

Query: 121 --------LDATRLHHPGP-----------TPLCSQP------------RSLEFPVTIMC 180
                   L   R H P P           T L   P            RS+EFPVTI C
Sbjct: 121 HGFITPAPLHYVRNHGPVPKADSSNWTIEITGLLENPTTFTLHQLVNDFRSIEFPVTITC 180

Query: 181 SGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGGDD 240
           SGNRRKEQNM KQTIGFHWGAAVTSTSLWRGV LREVLKRCGIFSR RGALY+WMEG DD
Sbjct: 181 SGNRRKEQNMFKQTIGFHWGAAVTSTSLWRGVSLREVLKRCGIFSRHRGALYVWMEGADD 240

Query: 241 LPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWL 300
           LPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWL
Sbjct: 241 LPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWL 300

Query: 301 KRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVITTPSHDE 360
           KRIIVTNRESDN+YHFMDNRVLPSHVDSELA+AEGWWYKPEYV+YEMNINSVITTPSHDE
Sbjct: 301 KRIIVTNRESDNHYHFMDNRVLPSHVDSELANAEGWWYKPEYVIYEMNINSVITTPSHDE 360

Query: 361 RLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYWCW 420
            L INSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETW+ICNLEYHEKPNKYGKYWCW
Sbjct: 361 MLSINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCW 420

Query: 421 IFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGE 480
           IFWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKPQ GE
Sbjct: 421 IFWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKPQNGE 480

Query: 481 IGIVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNN-NTTTTSKTFSMSEIKTHNSPE 540
           +GIVFRHPTLPGNQSGGWKEKDK NPT   S S+PTN+ N TT SKTFS SE+KTHN+PE
Sbjct: 481 LGIVFRHPTLPGNQSGGWKEKDKINPTDPPS-SKPTNDKNKTTASKTFSRSEVKTHNTPE 540

Query: 541 SAWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGDLL 600
           S+WIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAI ML+DY IGDLL
Sbjct: 541 SSWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLQDYIIGDLL 600

Query: 601 IQNDNKEMASSSTPA-QDVALVPREKIPVKLISKTSISHNTRIFRFALPSKDQILGLPVG 660
            +NDN+E  SSS    Q VALVP +KIPVKLISK SISHNTRIFRF L SKDQ+LGLPVG
Sbjct: 601 TENDNEEKTSSSEAVEQHVALVPGKKIPVKLISKISISHNTRIFRFGLSSKDQVLGLPVG 660

Query: 661 KHIFLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPL 720
           KHIFLCA ++GKLCMRAYTPSSSVDE+G+FDLVVK+YFKNVHP FPNGGLMSQ+LDSLPL
Sbjct: 661 KHIFLCAIIEGKLCMRAYTPSSSVDEMGFFDLVVKVYFKNVHPDFPNGGLMSQYLDSLPL 720

Query: 721 GSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADET 780
           GSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP D+T
Sbjct: 721 GSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPQDKT 780

Query: 781 EMFLVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLREHV 840
           EM++VYANRTEEDILLREELDTWAKE ERLKIWYVVK+GV EGWPYSVGYI E VLREH+
Sbjct: 781 EMYVVYANRTEEDILLREELDTWAKENERLKIWYVVKEGVKEGWPYSVGYIKESVLREHI 840

BLAST of Tan0018673 vs. NCBI nr
Match: NP_001267688.1 (nitrate reductase [NAD(P)H]-like [Cucumis sativus] >AFH74423.1 cucumber nitrate reductase 3 [Cucumis sativus] >KGN50981.1 hypothetical protein Csa_008642 [Cucumis sativus])

HSP 1 Score: 1494.9 bits (3869), Expect = 0.0e+00
Identity = 734/877 (83.69%), Postives = 777/877 (88.60%), Query Frame = 0

Query: 1   MAAAVDHKTLDLEPASNS-NFPVQRCNSFRSSSFKFKRAAEKSFSVDDDDSSFDRDDENI 60
           MA AV+HKTLDLEP S++ NFPVQRCNSFRS+SFKFKR AEK   +DDDDSSFDRD+ENI
Sbjct: 1   MATAVNHKTLDLEPVSSTGNFPVQRCNSFRSNSFKFKRQAEKPLLIDDDDSSFDRDEENI 60

Query: 61  NYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDR------ 120
           +YFRDLIRKGKEEIEPSILDPRD+GTADSGIMRNPCMVRLTGKHPFNSEPPLD       
Sbjct: 61  DYFRDLIRKGKEEIEPSILDPRDQGTADSGIMRNPCMVRLTGKHPFNSEPPLDHLMRHGF 120

Query: 121 -----LDATRLHHPGP-----------TPLCSQP------------RSLEFPVTIMCSGN 180
                L   R H P P           T L   P            RSLEFPVTIMCSGN
Sbjct: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNNFRSLEFPVTIMCSGN 180

Query: 181 RRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGGDDLPG 240
           RRKEQNM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALY+WMEG D+LPG
Sbjct: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYVWMEGADNLPG 240

Query: 241 GGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWLKRI 300
           GGGCSYGTSIRREMAMDPSRDIIIAY QNGDP+PPDHGFPVRMIVPGFTGGRMVKWLKRI
Sbjct: 241 GGGCSYGTSIRREMAMDPSRDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300

Query: 301 IVTNRESDNYYHFMDNRVLPSHVD-SELAHAEGWWYKPEYVVYEMNINSVITTPSHDERL 360
           IVTNRESDNYYHFMDNRVLPSHVD SELAHAEGWWYKPEYV+YEMNINSVITTPSH+E L
Sbjct: 301 IVTNRESDNYYHFMDNRVLPSHVDSSELAHAEGWWYKPEYVIYEMNINSVITTPSHNETL 360

Query: 361 PINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYWCWIF 420
            INS+TT STYTLRGYAYSGGGKKVTRVQVTLDGGETW+ICNLEYHEKPNKYGKYWCWIF
Sbjct: 361 LINSETTLSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIF 420

Query: 421 WSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIG 480
           WSV+VEI+DLFGSK+IAVRAWDET NTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIG
Sbjct: 421 WSVDVEILDLFGSKQIAVRAWDETTNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIG 480

Query: 481 IVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNNNTTTTSKTFSMSEIKTHNSPESAW 540
           IVFRHPT+ GN +GGW+EKDK NPT   SPSQ  +N +TT   TFSMSE+KTHNS ESAW
Sbjct: 481 IVFRHPTVAGNNAGGWQEKDKPNPTDE-SPSQQKDNKSTT---TFSMSEVKTHNSSESAW 540

Query: 541 IVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGDLLIQN 600
           IVV  HVYDCTPFLK HPGGADSILINAGTDCTEEF+AIHSDKAIKMLEDYKIGDLLI N
Sbjct: 541 IVVQNHVYDCTPFLKDHPGGADSILINAGTDCTEEFEAIHSDKAIKMLEDYKIGDLLILN 600

Query: 601 DNKEMASS-STPAQDVALVPREKIPVKLISKTSISHNTRIFRFALPSKDQILGLPVGKHI 660
           DNK+  SS     Q VAL+PREKIPVKLISKT+ISHNTRIFRF L S+DQ LGLPVGKHI
Sbjct: 601 DNKDATSSPKAVTQHVALIPREKIPVKLISKTTISHNTRIFRFGLSSEDQRLGLPVGKHI 660

Query: 661 FLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPLGST 720
           FLCA VDGKLCMRAYTPSS+VD+ GYFDLVVKIYFKNVHP FPNGGLMSQ+LDSLPLGS 
Sbjct: 661 FLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSM 720

Query: 721 VDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADETEMF 780
           V+VKGPLGHIEYMGRGNF V+GKP+ AKKLAMVAGGTGITPIYQVVQAILKDP DETEMF
Sbjct: 721 VEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMF 780

Query: 781 LVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLREHVPMG 840
           +VYANR+EEDILLREEL+TWAKE ERLKIWYVVKDGV EGWPYSVGYI E +LREH+PMG
Sbjct: 781 VVYANRSEEDILLREELETWAKENERLKIWYVVKDGVQEGWPYSVGYIREDILREHIPMG 840

BLAST of Tan0018673 vs. ExPASy TrEMBL
Match: A0A6J1FF98 (Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444943 PE=3 SV=1)

HSP 1 Score: 1495.7 bits (3871), Expect = 0.0e+00
Identity = 732/880 (83.18%), Postives = 777/880 (88.30%), Query Frame = 0

Query: 1   MAAAVDHKTLDLEPASNSNFPVQRCNSFRSSSFKFKRAAEKSFSV----DDDDSSFDRDD 60
           MAA VDHKTLD+EP +N +F  QRCNSFRS SFK KR  E + ++    DDDDSSFDR D
Sbjct: 1   MAAVVDHKTLDMEPPTNGSFLAQRCNSFRSPSFKIKRQPEITLTIDDDDDDDDSSFDRHD 60

Query: 61  ENINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDR--- 120
           +N +YFRDLIRKGKEEIE SILDPRDEGTAD+GI+RNP M+RLTGKHPFNSEPPLDR   
Sbjct: 61  DNNDYFRDLIRKGKEEIELSILDPRDEGTADNGILRNPSMIRLTGKHPFNSEPPLDRLMR 120

Query: 121 --------LDATRLHHPGP-----------TPLCSQP------------RSLEFPVTIMC 180
                   L   R H P P           T L   P            RS+EFPVTI C
Sbjct: 121 HGFITPAPLHYVRNHGPVPKADSSNWTIEITGLLENPTTFTLHQLVNDFRSIEFPVTITC 180

Query: 181 SGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGGDD 240
           SGNRRKEQNM KQTIGFHWGAAVTSTSLWRGV LREVLKRCGIFSR RGALY+WMEG DD
Sbjct: 181 SGNRRKEQNMFKQTIGFHWGAAVTSTSLWRGVSLREVLKRCGIFSRHRGALYVWMEGADD 240

Query: 241 LPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWL 300
           LPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWL
Sbjct: 241 LPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWL 300

Query: 301 KRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVITTPSHDE 360
           KRIIVTNRESDN+YHFMDNRVLPSHVDSELA+AEGWWYKPEYV+YEMNINSVITTPSHDE
Sbjct: 301 KRIIVTNRESDNHYHFMDNRVLPSHVDSELANAEGWWYKPEYVIYEMNINSVITTPSHDE 360

Query: 361 RLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYWCW 420
            L INSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETW+ICNLEYHEKPNKYGKYWCW
Sbjct: 361 MLSINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCW 420

Query: 421 IFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGE 480
           IFWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKPQ GE
Sbjct: 421 IFWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKPQNGE 480

Query: 481 IGIVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNN-NTTTTSKTFSMSEIKTHNSPE 540
           +GIVFRHPTLPGNQSGGWKEKDK NPT   S S+PTN+ N TT SKTFS SE+KTHN+PE
Sbjct: 481 LGIVFRHPTLPGNQSGGWKEKDKINPTDPPS-SKPTNDKNKTTASKTFSRSEVKTHNTPE 540

Query: 541 SAWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGDLL 600
           S+WIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAI ML+DY IGDLL
Sbjct: 541 SSWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLQDYIIGDLL 600

Query: 601 IQNDNKEMASSSTPA-QDVALVPREKIPVKLISKTSISHNTRIFRFALPSKDQILGLPVG 660
            +NDN+E  SSS    Q VALVP +KIPVKLISK SISHNTRIFRF L SKDQ+LGLPVG
Sbjct: 601 TENDNEEKTSSSEAVEQHVALVPGKKIPVKLISKISISHNTRIFRFGLSSKDQVLGLPVG 660

Query: 661 KHIFLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPL 720
           KHIFLCA ++GKLCMRAYTPSSSVDE+G+FDLVVK+YFKNVHP FPNGGLMSQ+LDSLPL
Sbjct: 661 KHIFLCAIIEGKLCMRAYTPSSSVDEMGFFDLVVKVYFKNVHPDFPNGGLMSQYLDSLPL 720

Query: 721 GSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADET 780
           GSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP D+T
Sbjct: 721 GSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPQDKT 780

Query: 781 EMFLVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLREHV 840
           EM++VYANRTEEDILLREELDTWAKE ERLKIWYVVK+GV EGWPYSVGYI E VLREH+
Sbjct: 781 EMYVVYANRTEEDILLREELDTWAKENERLKIWYVVKEGVKEGWPYSVGYIKESVLREHI 840

BLAST of Tan0018673 vs. ExPASy TrEMBL
Match: I0CCB0 (Nitrate reductase OS=Cucumis sativus OX=3659 GN=NR3 PE=2 SV=1)

HSP 1 Score: 1494.9 bits (3869), Expect = 0.0e+00
Identity = 734/877 (83.69%), Postives = 777/877 (88.60%), Query Frame = 0

Query: 1   MAAAVDHKTLDLEPASNS-NFPVQRCNSFRSSSFKFKRAAEKSFSVDDDDSSFDRDDENI 60
           MA AV+HKTLDLEP S++ NFPVQRCNSFRS+SFKFKR AEK   +DDDDSSFDRD+ENI
Sbjct: 1   MATAVNHKTLDLEPVSSTGNFPVQRCNSFRSNSFKFKRQAEKPLLIDDDDSSFDRDEENI 60

Query: 61  NYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDR------ 120
           +YFRDLIRKGKEEIEPSILDPRD+GTADSGIMRNPCMVRLTGKHPFNSEPPLD       
Sbjct: 61  DYFRDLIRKGKEEIEPSILDPRDQGTADSGIMRNPCMVRLTGKHPFNSEPPLDHLMRHGF 120

Query: 121 -----LDATRLHHPGP-----------TPLCSQP------------RSLEFPVTIMCSGN 180
                L   R H P P           T L   P            RSLEFPVTIMCSGN
Sbjct: 121 ITPAPLHYVRNHGPVPKTDWSSWTIDVTGLVENPTSFTLDQLVNNFRSLEFPVTIMCSGN 180

Query: 181 RRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGGDDLPG 240
           RRKEQNM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALY+WMEG D+LPG
Sbjct: 181 RRKEQNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYVWMEGADNLPG 240

Query: 241 GGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWLKRI 300
           GGGCSYGTSIRREMAMDPSRDIIIAY QNGDP+PPDHGFPVRMIVPGFTGGRMVKWLKRI
Sbjct: 241 GGGCSYGTSIRREMAMDPSRDIIIAYKQNGDPLPPDHGFPVRMIVPGFTGGRMVKWLKRI 300

Query: 301 IVTNRESDNYYHFMDNRVLPSHVD-SELAHAEGWWYKPEYVVYEMNINSVITTPSHDERL 360
           IVTNRESDNYYHFMDNRVLPSHVD SELAHAEGWWYKPEYV+YEMNINSVITTPSH+E L
Sbjct: 301 IVTNRESDNYYHFMDNRVLPSHVDSSELAHAEGWWYKPEYVIYEMNINSVITTPSHNETL 360

Query: 361 PINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYWCWIF 420
            INS+TT STYTLRGYAYSGGGKKVTRVQVTLDGGETW+ICNLEYHEKPNKYGKYWCWIF
Sbjct: 361 LINSETTLSTYTLRGYAYSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWIF 420

Query: 421 WSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIG 480
           WSV+VEI+DLFGSK+IAVRAWDET NTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIG
Sbjct: 421 WSVDVEILDLFGSKQIAVRAWDETTNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEIG 480

Query: 481 IVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNNNTTTTSKTFSMSEIKTHNSPESAW 540
           IVFRHPT+ GN +GGW+EKDK NPT   SPSQ  +N +TT   TFSMSE+KTHNS ESAW
Sbjct: 481 IVFRHPTVAGNNAGGWQEKDKPNPTDE-SPSQQKDNKSTT---TFSMSEVKTHNSSESAW 540

Query: 541 IVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGDLLIQN 600
           IVV  HVYDCTPFLK HPGGADSILINAGTDCTEEF+AIHSDKAIKMLEDYKIGDLLI N
Sbjct: 541 IVVQNHVYDCTPFLKDHPGGADSILINAGTDCTEEFEAIHSDKAIKMLEDYKIGDLLILN 600

Query: 601 DNKEMASS-STPAQDVALVPREKIPVKLISKTSISHNTRIFRFALPSKDQILGLPVGKHI 660
           DNK+  SS     Q VAL+PREKIPVKLISKT+ISHNTRIFRF L S+DQ LGLPVGKHI
Sbjct: 601 DNKDATSSPKAVTQHVALIPREKIPVKLISKTTISHNTRIFRFGLSSEDQRLGLPVGKHI 660

Query: 661 FLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPLGST 720
           FLCA VDGKLCMRAYTPSS+VD+ GYFDLVVKIYFKNVHP FPNGGLMSQ+LDSLPLGS 
Sbjct: 661 FLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSM 720

Query: 721 VDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADETEMF 780
           V+VKGPLGHIEYMGRGNF V+GKP+ AKKLAMVAGGTGITPIYQVVQAILKDP DETEMF
Sbjct: 721 VEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMF 780

Query: 781 LVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLREHVPMG 840
           +VYANR+EEDILLREEL+TWAKE ERLKIWYVVKDGV EGWPYSVGYI E +LREH+PMG
Sbjct: 781 VVYANRSEEDILLREELETWAKENERLKIWYVVKDGVQEGWPYSVGYIREDILREHIPMG 840

BLAST of Tan0018673 vs. ExPASy TrEMBL
Match: A0A6J1FFD6 (Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444945 PE=3 SV=1)

HSP 1 Score: 1488.8 bits (3853), Expect = 0.0e+00
Identity = 729/879 (82.94%), Postives = 776/879 (88.28%), Query Frame = 0

Query: 1   MAAAVDHKTLDLEPASNSNFPVQRCNSFRSSSFKFKRAAEKSFSV---DDDDSSFDRDDE 60
           MAA VD K LD+E ASN  FPVQRCNSFRSSSFK KR  E S S+   DDDDSSFDR+DE
Sbjct: 1   MAATVDRKALDVETASNGGFPVQRCNSFRSSSFKLKRKPEISLSIDDDDDDDSSFDRNDE 60

Query: 61  NINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDR---- 120
           N+NY+R+LIRKGKEEIEPSILDPRDEGTAD+G+MRNP ++RLTGKHP N+EPPLDR    
Sbjct: 61  NVNYYRELIRKGKEEIEPSILDPRDEGTADNGVMRNPSILRLTGKHPLNAEPPLDRLMRH 120

Query: 121 -------LDATRLHHPGP-----------TPLCSQP------------RSLEFPVTIMCS 180
                  L   R H P P           T L   P            RS+EFPVTIMCS
Sbjct: 121 GFITPAPLHYVRNHGPVPKVDSSNWTVEITGLVQNPTSYTLHQLLNDFRSIEFPVTIMCS 180

Query: 181 GNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGGDDL 240
            NRRKE NM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSR RGALYIWMEG DDL
Sbjct: 181 ANRRKELNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRHRGALYIWMEGADDL 240

Query: 241 PGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWLK 300
           PGGGGC+YGTSI REMAMDPSRDIIIAYMQNG+PIPPDHGFPVRMIVPGFTGGRMVKWLK
Sbjct: 241 PGGGGCTYGTSISREMAMDPSRDIIIAYMQNGEPIPPDHGFPVRMIVPGFTGGRMVKWLK 300

Query: 301 RIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVITTPSHDER 360
           RIIVTNRESDNYYHFMDNRVLPSHVDSELA+AEGWWYKPEYVVYEMNINSVITTPSHDE 
Sbjct: 301 RIIVTNRESDNYYHFMDNRVLPSHVDSELANAEGWWYKPEYVVYEMNINSVITTPSHDET 360

Query: 361 LPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYWCWI 420
           L I+S+ TQSTYTLRGYA+SGGGKKVTRVQVTLDGGETW+ICNLEYHEKPNKYGKYWCWI
Sbjct: 361 LLISSKKTQSTYTLRGYAFSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWI 420

Query: 421 FWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEI 480
           FWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKPQ GE+
Sbjct: 421 FWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKPQNGEL 480

Query: 481 GIVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNN-NTTTTSKTFSMSEIKTHNSPES 540
           GIVFRHPTLPG+QSGGWK KDK NPT   S S+PTN+ N TT SKTFSMSE+KTHN+PES
Sbjct: 481 GIVFRHPTLPGHQSGGWKGKDKINPTDPPS-SKPTNDKNKTTASKTFSMSEVKTHNTPES 540

Query: 541 AWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGDLLI 600
           +WIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAI MLEDY IGDLL 
Sbjct: 541 SWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLEDYIIGDLLT 600

Query: 601 QNDNKEMASSSTPA-QDVALVPREKIPVKLISKTSISHNTRIFRFALPSKDQILGLPVGK 660
           +NDN+E  SSS    Q VALVP +KIPVKLISK SISHNTRIFRF L SKDQ+LGLPVGK
Sbjct: 601 ENDNEEKTSSSEAVEQHVALVPGKKIPVKLISKISISHNTRIFRFGLSSKDQVLGLPVGK 660

Query: 661 HIFLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPLG 720
           HIFLCA ++GKLCMRAYTPSSSVDE+ +FDLVVK+YFKNVHP FPNGGLMSQ+LDSLPLG
Sbjct: 661 HIFLCAIIEGKLCMRAYTPSSSVDEMSFFDLVVKVYFKNVHPDFPNGGLMSQYLDSLPLG 720

Query: 721 STVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADETE 780
           STVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP D+TE
Sbjct: 721 STVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPQDKTE 780

Query: 781 MFLVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLREHVP 840
           M++VYANRTEEDILLREELDTWAKE ERLKIWYVVK+GV EGWPYSVGYI E VLREH+P
Sbjct: 781 MYVVYANRTEEDILLREELDTWAKENERLKIWYVVKEGVKEGWPYSVGYIKESVLREHIP 840

BLAST of Tan0018673 vs. ExPASy TrEMBL
Match: A0A6J1FK45 (Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444944 PE=3 SV=1)

HSP 1 Score: 1486.1 bits (3846), Expect = 0.0e+00
Identity = 728/879 (82.82%), Postives = 774/879 (88.05%), Query Frame = 0

Query: 1   MAAAVDHKTLDLEPASNSNFPVQRCNSFRSSSFKFKRAAEKSFSV---DDDDSSFDRDDE 60
           MAA VD K LD+E ASN  FPVQRCNSFRSSSFK KR  E S S+   DDDDSSFDR+DE
Sbjct: 1   MAATVDRKALDVETASNGGFPVQRCNSFRSSSFKLKRKPEISLSIDDDDDDDSSFDRNDE 60

Query: 61  NINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDR---- 120
           N+NY+R+LIRKGKEEIEPSILDPRDEGTAD+G+MRNP ++RLTGKHP N+EPPLDR    
Sbjct: 61  NVNYYRELIRKGKEEIEPSILDPRDEGTADNGVMRNPSILRLTGKHPLNAEPPLDRLMRH 120

Query: 121 -------LDATRLHHPGP-----------TPLCSQP------------RSLEFPVTIMCS 180
                  L   R H P P           T L   P            RS+EFPVTIMCS
Sbjct: 121 GFITPAPLHYVRNHGPVPKVDSSNWTVEITGLVQNPTSYTLHQLLNDFRSIEFPVTIMCS 180

Query: 181 GNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGGDDL 240
            NRRKE NM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSR RGALYIWMEG DDL
Sbjct: 181 ANRRKELNMVKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRHRGALYIWMEGADDL 240

Query: 241 PGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWLK 300
           PGGGGC+YGTSIRREMAMDPSRDIIIAYMQNG+ IPPDHGFPVRMIVPGFTGGRMVKWLK
Sbjct: 241 PGGGGCTYGTSIRREMAMDPSRDIIIAYMQNGEHIPPDHGFPVRMIVPGFTGGRMVKWLK 300

Query: 301 RIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVITTPSHDER 360
           RIIVTNRES+NYYHFMDNRVLPSHVDSELA+AEGWWYKPEYVVYEMNINSVITTPSHDE 
Sbjct: 301 RIIVTNRESNNYYHFMDNRVLPSHVDSELANAEGWWYKPEYVVYEMNINSVITTPSHDET 360

Query: 361 LPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYWCWI 420
           L I+S+ TQSTYTLRGYA+SGGGKKVTRVQVTLDGGETW+ICNLEYHEKPNKYGKYWCWI
Sbjct: 361 LLISSKKTQSTYTLRGYAFSGGGKKVTRVQVTLDGGETWKICNLEYHEKPNKYGKYWCWI 420

Query: 421 FWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGEI 480
           FWSVEVEIV+LFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSW+RIKIN+CKPQ GE+
Sbjct: 421 FWSVEVEIVELFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWFRIKINVCKPQNGEL 480

Query: 481 GIVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNN-NTTTTSKTFSMSEIKTHNSPES 540
           GIVFRHPTLPGNQSGGWKEKDK NPT   S S+PTN+ N TT SKTFSMSE+KTHN+PES
Sbjct: 481 GIVFRHPTLPGNQSGGWKEKDKINPTDPPS-SKPTNDKNKTTASKTFSMSEVKTHNTPES 540

Query: 541 AWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGDLLI 600
           +WIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAI MLEDY IGDLL 
Sbjct: 541 SWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAINMLEDYIIGDLLT 600

Query: 601 QNDNKEMASSSTPA-QDVALVPREKIPVKLISKTSISHNTRIFRFALPSKDQILGLPVGK 660
           +NDN+E  SSS    Q VALVP +KIPVKLISK SISHNTRIFRF L SKDQ+LGLPVGK
Sbjct: 601 ENDNEEKTSSSEAVEQHVALVPGKKIPVKLISKISISHNTRIFRFGLSSKDQVLGLPVGK 660

Query: 661 HIFLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPLG 720
           HIFLCA ++GKLCMRAYTPSSSVDE G+FDLVVK+YFKNVHP FPNGGLMSQ+LDSLPLG
Sbjct: 661 HIFLCAIIEGKLCMRAYTPSSSVDETGFFDLVVKVYFKNVHPDFPNGGLMSQYLDSLPLG 720

Query: 721 STVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADETE 780
           STVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDP D+TE
Sbjct: 721 STVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPEDKTE 780

Query: 781 MFLVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLREHVP 840
           M++VYANRTEEDILLREELDTWAKE ERLKIWYVVKDGV EGWPYSVG+I E +LREH+P
Sbjct: 781 MYVVYANRTEEDILLREELDTWAKENERLKIWYVVKDGVKEGWPYSVGHINESILREHIP 840

BLAST of Tan0018673 vs. ExPASy TrEMBL
Match: A0A6J1CIG4 (Nitrate reductase OS=Momordica charantia OX=3673 GN=LOC111011787 PE=3 SV=1)

HSP 1 Score: 1485.7 bits (3845), Expect = 0.0e+00
Identity = 726/883 (82.22%), Postives = 779/883 (88.22%), Query Frame = 0

Query: 1   MAAAVDHKTL---DLEPASNSNFPVQRCNSFRSSSFKFKRAAEKSFSVDDDDSSFDRDDE 60
           MAAAVDHKT    DLEPASN+ FPV RCNSFRS+SFK KR AEKS ++DD+DSSFD D +
Sbjct: 1   MAAAVDHKTPGFGDLEPASNAKFPVPRCNSFRSASFKLKRPAEKSLTIDDEDSSFDGDQD 60

Query: 61  NINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKHPFNSEPPLDR---- 120
             +Y+RDLIRKGK+EIEPSILDPRDEGTADSGIMRNP M+RLTGKHPFNSEPPLD     
Sbjct: 61  QNSYYRDLIRKGKDEIEPSILDPRDEGTADSGIMRNPSMIRLTGKHPFNSEPPLDHLMRH 120

Query: 121 -------LDATRLHHPGP-----------TPLCSQP------------RSLEFPVTIMCS 180
                  L   R H P P           T L   P            RS+EFPVTIMCS
Sbjct: 121 GFITPAPLHYVRNHGPVPKTDWASWTIDVTGLVQTPTSFTLDQLITGFRSVEFPVTIMCS 180

Query: 181 GNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYIWMEGGDDL 240
           GNRRKEQNM KQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALY+WMEGGDDL
Sbjct: 181 GNRRKEQNMMKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGIFSRQRGALYVWMEGGDDL 240

Query: 241 PGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTGGRMVKWLK 300
           PGGGGCSYGTSIRREMAMDPSRDIIIAY QNG+ +PPDHGFPVRMIVPGFTGGRMVKWLK
Sbjct: 241 PGGGGCSYGTSIRREMAMDPSRDIIIAYKQNGEDLPPDHGFPVRMIVPGFTGGRMVKWLK 300

Query: 301 RIIVTNRESDNYYHFMDNRVLPSHV-DSELAHAEGWWYKPEYVVYEMNINSVITTPSHDE 360
           RIIVTNRESDNYYHFMDNRVLPSHV DSELA+AEGWWYKPEYV+YEMNINSVITTPSHDE
Sbjct: 301 RIIVTNRESDNYYHFMDNRVLPSHVLDSELANAEGWWYKPEYVIYEMNINSVITTPSHDE 360

Query: 361 RLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQICNLEYHEKPNKYGKYWCW 420
            L IN+Q TQ+TYTLRGYA+SGGGKKVTRVQVTLDGGETW++CNLEYHEKPNKYGKYWCW
Sbjct: 361 TLTINAQATQTTYTLRGYAFSGGGKKVTRVQVTLDGGETWRMCNLEYHEKPNKYGKYWCW 420

Query: 421 IFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGE 480
           IFWSVEVEIVDLF +KEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGE
Sbjct: 421 IFWSVEVEIVDLFRAKEIAVRAWDETNNTQPENLNWNLMGMMNNSWYRIKINICKPQKGE 480

Query: 481 IGIVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTNNNTTTTSKTFSMSEIKTHNSPES 540
           IGIVFRHPTL GNQSGGWKEK+K       +P  PT  NT  +SKTFSMSE+KTHNSPES
Sbjct: 481 IGIVFRHPTLAGNQSGGWKEKEKEKNQTDAAPG-PT-KNTIPSSKTFSMSEVKTHNSPES 540

Query: 541 AWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTEEFDAIHSDKAIKMLEDYKIGDLLI 600
           AWIVVHGHVYDCTPFLK HPGGADSILINAGTDCTEEFDAIHSDKA  MLEDY+IGDLLI
Sbjct: 541 AWIVVHGHVYDCTPFLKDHPGGADSILINAGTDCTEEFDAIHSDKAKNMLEDYRIGDLLI 600

Query: 601 QNDN-----KEMASSSTPAQDVALVPREKIPVKLISKTSISHNTRIFRFALPSKDQILGL 660
           +N++     KE+ S+ TP++ VALVPREKIPVKL+SKTSISHNTR+FRFALP +DQILGL
Sbjct: 601 ENNSSPSPIKELTSAQTPSRHVALVPREKIPVKLVSKTSISHNTRVFRFALPCEDQILGL 660

Query: 661 PVGKHIFLCATVDGKLCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDS 720
           PVGKHIFLCATVDGKLCMRAYTPSS+VD+VGYFDLVVK+YFKNVHP FPNGGLMSQ+LDS
Sbjct: 661 PVGKHIFLCATVDGKLCMRAYTPSSAVDQVGYFDLVVKVYFKNVHPNFPNGGLMSQYLDS 720

Query: 721 LPLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPA 780
           LPLGS VDVKGPLGHIEYMGRGNF VHGKP FAKKLAMVAGGTGITPIYQVVQAILKDP 
Sbjct: 721 LPLGSVVDVKGPLGHIEYMGRGNFMVHGKPTFAKKLAMVAGGTGITPIYQVVQAILKDPE 780

Query: 781 DETEMFLVYANRTEEDILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLR 840
           DETEMF+VYANRTEEDILLREELD WA++ E+LKIW+VVK+G GEGWPYSVGYITEG+LR
Sbjct: 781 DETEMFVVYANRTEEDILLREELDAWAEKHEKLKIWHVVKEGGGEGWPYSVGYITEGILR 840

BLAST of Tan0018673 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 538/861 (62.49%), Postives = 665/861 (77.24%), Query Frame = 0

Query: 48  DDSSFDRDDE----NINYFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLTGKH 107
           DDSS D +DE    N+ Y+++L++K   ++EPSILDPRDE TADS I RN  M+RLTGKH
Sbjct: 65  DDSSSDDEDESHNRNVPYYKELVKKSNSDLEPSILDPRDESTADSWIQRNSSMLRLTGKH 124

Query: 108 PFNSEPPLDRLDATRLHHPGPTP------------------------------------- 167
           PFN+E PL RL    +HH   TP                                     
Sbjct: 125 PFNAEAPLPRL----MHHGFITPVPLHYVRNHGAVPKANWSDWSIEITGLVKRPAKFTME 184

Query: 168 -LCSQPRSLEFPVTIMCSGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGI 227
            L S+  S EFPVT++C+GNRRKEQNM KQTIGF+WG+A  STSLW+G+PL E+L+RCGI
Sbjct: 185 ELISEFPSREFPVTLVCAGNRRKEQNMVKQTIGFNWGSAGVSTSLWKGIPLSEILRRCGI 244

Query: 228 FSRQRGALYIWMEGGDDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFP 287
           +SR+ GAL +  EG +DLPGGGG  YGTSI++EMAMDP+RDII+AYMQNG+ + PDHGFP
Sbjct: 245 YSRRGGALNVCFEGAEDLPGGGGSKYGTSIKKEMAMDPARDIILAYMQNGELLTPDHGFP 304

Query: 288 VRMIVPGFTGGRMVKWLKRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYV 347
           VR+IVPGF GGRMVKWLKRIIVT +ESD+YYH+ DNRVLPS VD+ELA++E WWYKPEY+
Sbjct: 305 VRVIVPGFIGGRMVKWLKRIIVTPQESDSYYHYKDNRVLPSLVDAELANSEAWWYKPEYI 364

Query: 348 VYEMNINSVITTPSHDERLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETWQIC 407
           + E+NINSVITTP H E LPIN+ TTQ  YTL+GYAYSGGGKKVTRV+VTLDGG+TW +C
Sbjct: 365 INELNINSVITTPGHAEILPINAFTTQKPYTLKGYAYSGGGKKVTRVEVTLDGGDTWSVC 424

Query: 408 NLEYHEKPNKYGKYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWNLMGMMN 467
            L++ EKPNKYGK+WCW FWS++VE++DL  +K++AVRAWDE+ NTQP+ L WNLMGMMN
Sbjct: 425 ELDHQEKPNKYGKFWCWCFWSLDVEVLDLLSAKDVAVRAWDESFNTQPDKLIWNLMGMMN 484

Query: 468 NSWYRIKINICKPQKGEIGIVFRHPTLPGNQSGGWKEKDK-------NNPTAAGSPSQPT 527
           N W+RI+ N+CKP +GEIGIVF HPT PGNQSGGW  K++       +N T   S S P 
Sbjct: 485 NCWFRIRTNVCKPHRGEIGIVFEHPTRPGNQSGGWMAKERQLEISSESNNTLKKSVSSPF 544

Query: 528 NNNTTTTSKTFSMSEIKTHNSPESAWIVVHGHVYDCTPFLKAHPGGADSILINAGTDCTE 587
            N   T SK +S+SE++ HN+ +SAWI+VHGH+YDCT FLK HPGG DSILINAGTDCTE
Sbjct: 545 MN---TASKMYSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTE 604

Query: 588 EFDAIHSDKAIKMLEDYKIGDLLI----QNDNKEMASSS------------TPAQDVALV 647
           EF+AIHSDKA K+LEDY+IG+L+      + N  +  +S            TP +++ALV
Sbjct: 605 EFEAIHSDKAKKLLEDYRIGELITTGYDSSPNVSVHGASNFGPLLAPIKELTPQKNIALV 664

Query: 648 -PREKIPVKLISKTSISHNTRIFRFALPSKDQILGLPVGKHIFLCATVDGKLCMRAYTPS 707
            PREKIPV+LI KTSISH+ R FRFALPS+DQ LGLPVGKH+F+CA ++ KLC+RAYTP+
Sbjct: 665 NPREKIPVRLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCANINDKLCLRAYTPT 724

Query: 708 SSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPLGSTVDVKGPLGHIEYMGRGNF 767
           S++D VG+ DLVVK+YFK+VHP+FPNGGLMSQ LDSLP+GS +D+KGPLGHIEY G+GNF
Sbjct: 725 SAIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNF 784

Query: 768 TVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADETEMFLVYANRTEEDILLREELD 827
            V GKPKFAKKLAM+AGGTGITPIYQ++Q+IL DP DETEM++VYANRTE+DIL+REEL+
Sbjct: 785 LVSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREELE 844

Query: 828 TWA-KETERLKIWYVVKDGVGEGWPYSVGYITEGVLREHVPMG-SPETLALVCGPPPMIE 841
            WA K  ERLKIWYVV +   EGW YS G+ITE VLREH+P G   E+LAL CGPPPMI+
Sbjct: 845 GWASKHKERLKIWYVV-EIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMIQ 904

BLAST of Tan0018673 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 550/927 (59.33%), Postives = 684/927 (73.79%), Query Frame = 0

Query: 1   MAAAVDHKT-LDLEPASN-------------SNFPVQRCNSFRSSSFKFKRAAEKSFSVD 60
           MAA+VD++    LEP  N             S+ P    N   + +   K A      V 
Sbjct: 1   MAASVDNRQYARLEPGLNGVVRSYKPPVPGRSDSPKAHQNQTTNQTVFLKPA-----KVH 60

Query: 61  DDDSSFDRDDENIN------YFRDLIRKGKEEIEPSILDPRDEGTADSGIMRNPCMVRLT 120
           DDD     +DEN        Y++++IRK   E+EPS+LDPRDE TADS I RNP MVRLT
Sbjct: 61  DDDEDVSSEDENETHNSNAVYYKEMIRKSNAELEPSVLDPRDEYTADSWIERNPSMVRLT 120

Query: 121 GKHPFNSEPPLDRLDATRLHHPGPTP---------------------------------- 180
           GKHPFNSE PL+RL    +HH   TP                                  
Sbjct: 121 GKHPFNSEAPLNRL----MHHGFITPVPLHYVRNHGHVPKAQWAEWTVEVTGFVKRPMKF 180

Query: 181 ----LCSQPRSLEFPVTIMCSGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKR 240
               L S+    EF  T++C+GNRRKEQNM K++ GF+WG+A  STS+WRGVPL +VL+R
Sbjct: 181 TMDQLVSEFAYREFAATLVCAGNRRKEQNMVKKSKGFNWGSAGVSTSVWRGVPLCDVLRR 240

Query: 241 CGIFSRQRGALYIWMEGGDDLPGGGGCS---YGTSIRREMAMDPSRDIIIAYMQNGDPIP 300
           CGIFSR+ GAL +  EG +DLPGG G +   YGTSI++E AMDPSRDII+AYMQNG+ + 
Sbjct: 241 CGIFSRKGGALNVCFEGSEDLPGGAGTAGSKYGTSIKKEYAMDPSRDIILAYMQNGEYLT 300

Query: 301 PDHGFPVRMIVPGFTGGRMVKWLKRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWW 360
           PDHGFPVR+I+PGF GGRMVKWLKRIIVT +ESDN+YHF DNRVLPS VD+ELA  EGWW
Sbjct: 301 PDHGFPVRIIIPGFIGGRMVKWLKRIIVTTKESDNFYHFKDNRVLPSLVDAELADEEGWW 360

Query: 361 YKPEYVVYEMNINSVITTPSHDERLPINSQTTQSTYTLRGYAYSGGGKKVTRVQVTLDGG 420
           YKPEY++ E+NINSVITTP H+E LPIN+ TTQ  YTL+GYAYSGGGKKVTRV+VT+DGG
Sbjct: 361 YKPEYIINELNINSVITTPCHEEILPINAFTTQRPYTLKGYAYSGGGKKVTRVEVTVDGG 420

Query: 421 ETWQICNLEYHEKPNKYGKYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLNWN 480
           ETW +C L++ EKPNKYGK+WCW FWS+EVE++DL  +KEIAVRAWDET NTQPE + WN
Sbjct: 421 ETWNVCALDHQEKPNKYGKFWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKMIWN 480

Query: 481 LMGMMNNSWYRIKINICKPQKGEIGIVFRHPTLPGNQSGGWKEKDKNNPTAAGSPSQPTN 540
           LMGMMNN W+R+K N+CKP KGEIGIVF HPTLPGN+SGGW  K+++   +A +P     
Sbjct: 481 LMGMMNNCWFRVKTNVCKPHKGEIGIVFEHPTLPGNESGGWMAKERHLEKSADAPPSLKK 540

Query: 541 NNTT----TTSKTFSMSEIKTHNSPESAWIVVHGHVYDCTPFLKAHPGGADSILINAGTD 600
           + +T    TT+K +SMSE+K HNS +S WI+VHGH+YDCT FL  HPGG+DSILINAGTD
Sbjct: 541 SVSTPFMNTTAKMYSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTD 600

Query: 601 CTEEFDAIHSDKAIKMLEDYKIGDLLIQNDNKEMAS-------------------SSTPA 660
           CTEEF+AIHSDKA KMLEDY+IG+L+    + + +S                    +TP 
Sbjct: 601 CTEEFEAIHSDKAKKMLEDYRIGELITTGYSSDSSSPNNSVHGSSAVFSLLAPIGEATPV 660

Query: 661 QDVALV-PREKIPVKLISKTSISHNTRIFRFALPSKDQILGLPVGKHIFLCATVDGKLCM 720
           +++ALV PR K+PV+L+ KTSISH+ R FRFALP +D +LGLPVGKHIFLCAT++ KLC+
Sbjct: 661 RNLALVNPRAKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATINDKLCL 720

Query: 721 RAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPLGSTVDVKGPLGHIEY 780
           RAYTPSS+VD VGYF+LVVKIYF  VHP+FPNGGLMSQ+LDSLP+GST+++KGPLGH+EY
Sbjct: 721 RAYTPSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEY 780

Query: 781 MGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADETEMFLVYANRTEEDIL 840
           +G+G+FTVHGKPKFA KLAM+AGGTGITP+YQ++QAILKDP DETEM+++YANRTEEDIL
Sbjct: 781 LGKGSFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDIL 840

BLAST of Tan0018673 vs. TAIR 10
Match: AT3G01910.1 (sulfite oxidase )

HSP 1 Score: 167.9 bits (424), Expect = 3.4e-41
Identity = 114/398 (28.64%), Postives = 173/398 (43.47%), Query Frame = 0

Query: 92  RNPCMVRLTGKHPFNSEPPLDRLDAT----------RLHHPGP------------TPLCS 151
           R+P + ++  K PFN+EPP   L ++          R H P P            T L  
Sbjct: 16  RHPSL-KVNAKEPFNAEPPRSALVSSYVTPVDLFYKRNHGPIPIVDHLQSYSVTLTGLIQ 75

Query: 152 QPRSL-----------EFPVTIMCSGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLRE 211
            PR L               T+ C+GNRR   +  +   G  W  +    ++W G  L +
Sbjct: 76  NPRKLFIKDIRSLPKYNVTATLQCAGNRRTAMSKVRNVRGVGWDVSAIGNAVWGGAKLAD 135

Query: 212 VLKRCGI----FSRQRGALYIWMEGGDDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQN 271
           VL+  GI     S   GA ++     D      G  Y  SI    A +P  D+++AY  N
Sbjct: 136 VLELVGIPKLTASTNLGARHVEFVSVDRCKEENGGPYKASITLSQATNPEADVLLAYEMN 195

Query: 272 GDPIPPDHGFPVRMIVPGFTGGRMVKWLKRIIVTNRESDNYYHFMDNRVLPSHVDSELAH 331
           G+ +  DHGFP+R++VPG  G R VKWL  I V   ES  ++   D ++ P  V+ +   
Sbjct: 196 GETLNRDHGFPLRVVVPGVIGARSVKWLDSINVIAEESQGFFMQKDYKMFPPSVNWD--- 255

Query: 332 AEGWWYKPEYVVYEMNINSVITTPSHDERLPINS---------QTTQSTYTLRGYAYSGG 391
                          NIN     P  D   P+ S                +++GYA SGG
Sbjct: 256 ---------------NINWSSRRPQMD--FPVQSAICSVEDVQMVKPGKVSIKGYAVSGG 315

Query: 392 GKKVTRVQVTLDGGETW------QICNLEYHEKPNKYGKYWCWIFWSVEVEIVDLFGSKE 436
           G+ + RV ++LDGG+ W      Q    +Y  + +   K W W+ +      +D+  + E
Sbjct: 316 GRGIERVDISLDGGKNWVEASRTQEPGKQYISEHSSSDK-WAWVLFEA---TIDVSQTTE 375

BLAST of Tan0018673 vs. TAIR 10
Match: AT3G01910.2 (sulfite oxidase )

HSP 1 Score: 157.1 bits (396), Expect = 6.0e-38
Identity = 94/317 (29.65%), Postives = 146/317 (46.06%), Query Frame = 0

Query: 140 IMCSGNRRKEQNMAKQTIGFHWGAAVTSTSLWRGVPLREVLKRCGI----FSRQRGALYI 199
           + C+GNRR   +  +   G  W  +    ++W G  L +VL+  GI     S   GA ++
Sbjct: 1   MQCAGNRRTAMSKVRNVRGVGWDVSAIGNAVWGGAKLADVLELVGIPKLTASTNLGARHV 60

Query: 200 WMEGGDDLPGGGGCSYGTSIRREMAMDPSRDIIIAYMQNGDPIPPDHGFPVRMIVPGFTG 259
                D      G  Y  SI    A +P  D+++AY  NG+ +  DHGFP+R++VPG  G
Sbjct: 61  EFVSVDRCKEENGGPYKASITLSQATNPEADVLLAYEMNGETLNRDHGFPLRVVVPGVIG 120

Query: 260 GRMVKWLKRIIVTNRESDNYYHFMDNRVLPSHVDSELAHAEGWWYKPEYVVYEMNINSVI 319
            R VKWL  I V   ES  ++   D ++ P  V+ +                  NIN   
Sbjct: 121 ARSVKWLDSINVIAEESQGFFMQKDYKMFPPSVNWD------------------NINWSS 180

Query: 320 TTPSHDERLPINS---------QTTQSTYTLRGYAYSGGGKKVTRVQVTLDGGETW---- 379
             P  D   P+ S                +++GYA SGGG+ + RV ++LDGG+ W    
Sbjct: 181 RRPQMD--FPVQSAICSVEDVQMVKPGKVSIKGYAVSGGGRGIERVDISLDGGKNWVEAS 240

Query: 380 --QICNLEYHEKPNKYGKYWCWIFWSVEVEIVDLFGSKEIAVRAWDETNNTQPENLN--W 436
             Q    +Y  + +   K W W+ +      +D+  + E+  +A D   N QPEN+   W
Sbjct: 241 RTQEPGKQYISEHSSSDK-WAWVLFEA---TIDVSQTTEVIAKAVDSAANVQPENVESVW 293

BLAST of Tan0018673 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 156.8 bits (395), Expect = 7.9e-38
Identity = 102/274 (37.23%), Postives = 146/274 (53.28%), Query Frame = 0

Query: 580 ALVPREKIPVKLISKTSISHNTRIFRFAL-PSKDQILGLPVGKHIFLCATV------DGK 639
           AL P + +  KL     +SHNT++FRF+  PS +  LGL V   +   A +        K
Sbjct: 72  ALNPDKWLEFKLQDTARVSHNTQLFRFSFDPSAE--LGLHVASCLLTRAPLGYNAEGKTK 131

Query: 640 LCMRAYTPSSSVDEVGYFDLVVKIYFKNVHPQFPNGGLMSQFLDSLPLGSTVDVKGPLGH 699
             +R YTP S  +  GYFDL++K+Y           G MSQ   SL  G  ++VKGP+  
Sbjct: 132 YVIRPYTPISDPEAKGYFDLLIKVY---------PDGKMSQHFASLKPGDVLEVKGPVEK 191

Query: 700 IEYMGRGNFTVHGKPKFAKKLAMVAGGTGITPIYQVVQAILKDPADETEMFLVYANRTEE 759
            +Y           P   K + M+AGG+GITP+ QV+ AI+K+P D T++ L+YAN + +
Sbjct: 192 FKY----------SPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPD 251

Query: 760 DILLREELDTWAKETERLKIWYVVKDGVGEGWPYSVGYITEGVLREHVPMGSPETLALVC 819
           DILL+++LD        LKI+Y V D   + W   VGYI++ +  + +P+ + +TL LVC
Sbjct: 252 DILLKQKLDVLQANHPNLKIFYTV-DNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVC 311

Query: 820 GPPPMIEL------------TVKPILKSLEYDLE 835
           GPP M+E              VK ILK L Y  E
Sbjct: 312 GPPGMMEHISGGKAPDWSQGEVKGILKELGYTEE 323