Tan0018335 (gene) Snake gourd v1

Overview
NameTan0018335
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionacidic mammalian chitinase-like
LocationLG08: 2894977 .. 2896740 (+)
RNA-Seq ExpressionTan0018335
SyntenyTan0018335
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCAAATTACAAAGTTCGTGAATGCTGGTTATTGGCTTGCCAGTACTGATGAGACACTCCCAGCTGCAAATATCGATGCTACACTTTTTAGCCATTTATTTGTTGGATATGTTGGTGTCAACTCCCAAACTTATGAAGTCACTATAGATCCTTCACAAGCTATCTTAATCAATCAATTTTCTTCAACTGTGAGACTGCGTAATCCTGGGGTCAAAACTCTTTTGTCCATTGCAGGAGGCAATGATGTATTTGCTAAAGTTGCTGCAGAGCCTGCAAATCGTGATGCCTTCATCAAATCCTCCATTGATGTAGCCAGAGCAGGAGAATTTGATGGCCTTGATCTTCAATGGCTATACCCATCTTCTCAAGATGAAATGGACAACTTTGAAACTCTGCTTAGTTTTTGGCACAAAGCTATTGTAGATGATGCTATCAATTTAAGCCTTCCACAGTTACTTCTTGTTGCCACGGTCTCCAACGTACCCTATCTTCAACACAACATTCAATATCCAGTTGCTGCAATCAAACAAAACTTAGATTGGGTGAACGTCCTTTCTTATGACTTTTACACCCCAACTTTTTCAAGTAAGACTACAGGACCATCCTCAGCATTCAACAATCCAAAGGCTAATGCTCTATCAGCACATTTTGGTATTCGATCTTGGACCCAAAGTCTTAACAATCTGCCTCATAAAAAAATTGTATTTGGAATTCCCTTCCATGGTTGGGCTTGGAAACTTGCCAACTCTCACCAACATGACGTGTTTTCTAAAGCCGATGGAGCAGCAAGTGGAGAATATATTTCTCCAAACGGTCAAATCTACTACTCTAATGTGAAGCAGTTTATAGATGATAACCAAGCTACAAATGTAAACAAGGACCCTAGGTTTTTAATTGCCTACACTTATTCTGGCACAACCTGGATTGCATATGAAAGTGAATATACCATTGCAGACAAGATTTCATTGGCAAAGTCTGACAACGTGCTTGGTTACTTTGCTTTCAACATCGCAGCTGATGATGTTGTTAACACTCTTGCTAATGCTGCCAAATCCAATTGGTGATATATTCGCCACCACCCTTCATATAGCCACCACTTTTACTCATTTCCCAACCTTATGTTGAAACATCAAATAAAATGCATGATCATGTGTGCTAGTGTGTCTTTCTAAACTATGTTGTTGATGGTTGTTTGTGTCTATATTATTTAAAGTTGTTCTTTTTATTATCTTCTACCCTTGTATGGGTTTTATTTTCAACCATCTAATAAAATTGCTAGTGTGTTTTTACCAACTTCTCTCTTTTTCCACCTCAAAATATGTTATTTCCTCTCTTTCAAATTGCCAGTGTTGCTAATTTAACATGGTTTGAGGAGCTTCTGACGAATTACACAATTCATACATCAATCAATAGAGCAAATATATATTTCCATCCTCTAAATAAAGATTTTTTTTTATTATTTGAGGAAGACTTGATCAATATAATTCAGCCGACAATTTAATACTCGACTGGATATATATGAGATAATTTATGAACATAACTTTTGTTCATATTGAATGGTTATAAGGGACTCTCAGACTATATTGAATAATTTTTTCTTATAATGGATTAATTATAATAGTCGTAAGTTGTCAAATCTGGATAAGATAGAAGTATGGTACGCACGTAGTTGTTTTTAGAATTGGTTAATATATTTATATTATTTAGTTTTATGCTAATAGATTATTTACGTAAGATTTTTCAATCAGTGGGATGTTTGA

mRNA sequence

ATGGCTCAAATTACAAAGTTCGTGAATGCTGGTTATTGGCTTGCCAGTACTGATGAGACACTCCCAGCTGCAAATATCGATGCTACACTTTTTAGCCATTTATTTGTTGGATATGTTGGTGTCAACTCCCAAACTTATGAAGTCACTATAGATCCTTCACAAGCTATCTTAATCAATCAATTTTCTTCAACTGTGAGACTGCGTAATCCTGGGGTCAAAACTCTTTTGTCCATTGCAGGAGGCAATGATGTATTTGCTAAAGTTGCTGCAGAGCCTGCAAATCGTGATGCCTTCATCAAATCCTCCATTGATGTAGCCAGAGCAGGAGAATTTGATGGCCTTGATCTTCAATGGCTATACCCATCTTCTCAAGATGAAATGGACAACTTTGAAACTCTGCTTAGTTTTTGGCACAAAGCTATTGTAGATGATGCTATCAATTTAAGCCTTCCACAGTTACTTCTTGTTGCCACGGTCTCCAACGTACCCTATCTTCAACACAACATTCAATATCCAGTTGCTGCAATCAAACAAAACTTAGATTGGGTGAACGTCCTTTCTTATGACTTTTACACCCCAACTTTTTCAACACATTTTGGTATTCGATCTTGGACCCAAAGTCTTAACAATCTGCCTCATAAAAAAATTGTATTTGGAATTCCCTTCCATGGTTGGGCTTGGAAACTTGCCAACTCTCACCAACATGACGTGTTTTCTAAAGCCGATGGAGCAGCAAGTGGAGAATATATTTCTCCAAACGGTCAAATCTACTACTCTAATGTGAAGCAGTTTATAGATGATAACCAAGCTACAAATGTAAACAAGGACCCTAGGTTTTTAATTGCCTACACTTATTCTGGCACAACCTGGATTGCATATGAAAGTGAATATACCATTGCAGACAAGATTTCATTGGCAAAGTCTGACAACGTGCTTGGTTACTTTGCTTTCAACATCGCAGCTGATGATGTTTGGGATGTTTGA

Coding sequence (CDS)

ATGGCTCAAATTACAAAGTTCGTGAATGCTGGTTATTGGCTTGCCAGTACTGATGAGACACTCCCAGCTGCAAATATCGATGCTACACTTTTTAGCCATTTATTTGTTGGATATGTTGGTGTCAACTCCCAAACTTATGAAGTCACTATAGATCCTTCACAAGCTATCTTAATCAATCAATTTTCTTCAACTGTGAGACTGCGTAATCCTGGGGTCAAAACTCTTTTGTCCATTGCAGGAGGCAATGATGTATTTGCTAAAGTTGCTGCAGAGCCTGCAAATCGTGATGCCTTCATCAAATCCTCCATTGATGTAGCCAGAGCAGGAGAATTTGATGGCCTTGATCTTCAATGGCTATACCCATCTTCTCAAGATGAAATGGACAACTTTGAAACTCTGCTTAGTTTTTGGCACAAAGCTATTGTAGATGATGCTATCAATTTAAGCCTTCCACAGTTACTTCTTGTTGCCACGGTCTCCAACGTACCCTATCTTCAACACAACATTCAATATCCAGTTGCTGCAATCAAACAAAACTTAGATTGGGTGAACGTCCTTTCTTATGACTTTTACACCCCAACTTTTTCAACACATTTTGGTATTCGATCTTGGACCCAAAGTCTTAACAATCTGCCTCATAAAAAAATTGTATTTGGAATTCCCTTCCATGGTTGGGCTTGGAAACTTGCCAACTCTCACCAACATGACGTGTTTTCTAAAGCCGATGGAGCAGCAAGTGGAGAATATATTTCTCCAAACGGTCAAATCTACTACTCTAATGTGAAGCAGTTTATAGATGATAACCAAGCTACAAATGTAAACAAGGACCCTAGGTTTTTAATTGCCTACACTTATTCTGGCACAACCTGGATTGCATATGAAAGTGAATATACCATTGCAGACAAGATTTCATTGGCAAAGTCTGACAACGTGCTTGGTTACTTTGCTTTCAACATCGCAGCTGATGATGTTTGGGATGTTTGA

Protein sequence

MAQITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQFSSTVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYPSSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLDWVNVLSYDFYTPTFSTHFGIRSWTQSLNNLPHKKIVFGIPFHGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIAYTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADDVWDV
Homology
BLAST of Tan0018335 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.6e-66
Identity = 142/339 (41.89%), Postives = 200/339 (59.00%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQFSSTVRL 67
           V A YW  +++   P  +ID++LF+HLF  +  +NSQT +VT+  +     + F+ TV+ 
Sbjct: 28  VKASYWFPASE--FPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 68  RNPGVKTLLSIAGG---NDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYPSSQ 127
           RNP VKTLLSI GG      +A +A+ P +R +FI SSI VAR+  F GLDL W YPSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 128 DEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATV--SNVPYLQHNIQYPVAAIKQNLDW 187
            EM NF TLL  W  A+V +A +   P+LLL A V  SN  Y   ++ YPV+A+  +LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 188 VNVLSYDFYTPTFST------------------HFGIRSWTQSLNNLPHKKIVFGIPFHG 247
           VN+++YDFY P +S                     G RSW Q+   LP KK V G P++G
Sbjct: 208 VNLMAYDFYGPGWSRVTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPYYG 267

Query: 248 WAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIAYT 307
           +AW+L N++ H  ++   GAA    ISP+G I Y  +++FI DN AT V  +   +  Y 
Sbjct: 268 YAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGATTV-YNSTVVGDYC 327

Query: 308 YSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y+GT WI Y+   +I  K+  AK   +LGYF++++ ADD
Sbjct: 328 YAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADD 354

BLAST of Tan0018335 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.5e-45
Identity = 115/337 (34.12%), Postives = 177/337 (52.52%), Query Frame = 0

Query: 12  YWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQFSSTVRLRNPG 71
           YWL   +   P   +D +LF+H+F  ++  N+ TY++ I  SQ + +N F+ T + ++P 
Sbjct: 39  YWL--ENPFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKTKSPP 98

Query: 72  VKTLLSIAG---GNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYPSSQDEMD 131
             TL SI G    + + A +A++P    AFI S+IDVAR   FDG+DL W +P +  EM+
Sbjct: 99  AATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNTKEMN 158

Query: 132 NFETLLSFWHKAIVDDAINLSLPQLLLVATV---SNVPYLQHNIQYPVAAIKQNLDWVNV 191
           +   +L  W KAI D+      P LLL A V    N         YPV +I +NLDWVNV
Sbjct: 159 DLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLDWVNV 218

Query: 192 LSYDFYTP----------TF------STHFGIRSWTQSLNNLPHKKIVFGIPFHGWAWKL 251
           +SY+   P          TF      S   G+ SW  S   +  +K+V G+P +G +WKL
Sbjct: 219 MSYELRGPRSNKTGAPSGTFDPKSNVSVVSGLLSWIHS--GVVPEKLVMGMPLYGKSWKL 278

Query: 252 ANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIAYTYSGTT 311
            + + H + + + G+  G     NG + Y  V  F +  ++  V  D      Y+YSG+T
Sbjct: 279 RDPNVHGIGAPSVGSGPG----VNGLMAYFQVLDF-NRQKSAKVEYDVDTASVYSYSGST 338

Query: 312 WIAYESEYTIADKISLAKSDNVLGYFAFNIAADDVWD 327
           WI Y++ +T++ K+  A++  + GYF F +A  D  D
Sbjct: 339 WIGYDNPFTVSIKVGFAQALKLRGYF-FWVAGLDTLD 365

BLAST of Tan0018335 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 176.8 bits (447), Expect = 4.0e-43
Identity = 115/343 (33.53%), Postives = 179/343 (52.19%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQFSSTVRL 67
           V A YW A +D     ++ID   F+H++  +V    +++ + I  S      ++     +
Sbjct: 55  VRAAYWPAWSD--FSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDGIHNI 114

Query: 68  RNPGVKTLLSIAGGND---VFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYPSSQ 127
           R P V TLLSI GG +   +F+++A+   NR +FI S+I VAR  EF+GLDL W +P  +
Sbjct: 115 R-PRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDE 174

Query: 128 DEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATV---SNVPYLQHNIQ-YPVAAIKQNL 187
            +M N   LL  W+KA+V +A      +LLL + V   S +  + +  + YPV AI++ L
Sbjct: 175 KDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIRKYL 234

Query: 188 DWVNVLSYDF--------------YTP--TFSTHFGIRSWTQSLNNLPHKKIVFGIPFHG 247
           DW + + +D+              Y P    ST +GI SW  S   +P +K+V G+P +G
Sbjct: 235 DWASPMCFDYNGAWANETGFNAALYDPNSNISTKYGIGSWIGS--GVPAEKLVMGLPLYG 294

Query: 248 WAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIAYT 307
            AW+L + + H V +KA G A    +  +G + Y  +  F  D  A  V  D   +  Y+
Sbjct: 295 RAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDEILVFNKDTGA-KVVYDEVAVSFYS 354

Query: 308 YSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADDVWDV 328
           YSGTTWI Y+   +I  K+  A+S  + GYF + I  D  W +
Sbjct: 355 YSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDWTI 387

BLAST of Tan0018335 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 169.1 bits (427), Expect = 8.4e-41
Identity = 109/337 (32.34%), Postives = 168/337 (49.85%), Query Frame = 0

Query: 5   TKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQ----AILINQ 64
           T  V   YW+   +   P ++ID +LF+H+F  +V  N  TY++  +       A  +  
Sbjct: 34  TTRVKGIYWI--ENPLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTT 93

Query: 65  FSSTVRLRNPGVKTLLSIAG---GNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQ 124
           F++T + + P + TLLSI G    + +FA +A++P  R  FI S+I VAR   FDG+D  
Sbjct: 94  FTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFD 153

Query: 125 WLYPSSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATV---SNVPYLQHNIQYPVA 184
           W +P++  EM++   LL  W +AI D+  + S P LLL A V    N         YPV 
Sbjct: 154 WEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVD 213

Query: 185 AIKQNLDWVNVLSYDF----------------YTPTFSTHFGIRSWTQSLNNLPHKKIVF 244
           +I +NLDWVNV+SYD                      S   G+ SW +    +  +KIV 
Sbjct: 214 SINKNLDWVNVMSYDLRGSGSNVTGAPSGMFDSKSNVSVVSGLFSWIR--GGVAPEKIVM 273

Query: 245 GIPFHGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPR 304
           G+P +G +WKL + + H + +   G   G     +G + Y  V  F +      V  D  
Sbjct: 274 GMPLYGKSWKLQDPNVHGIGAPNVGPGPG----VDGGMAYFQVVDF-NKQMGAKVVYDKE 333

Query: 305 FLIAYTYSGTTWIAYESEYTIADKISLAKSDNVLGYF 316
               Y+YSG+TWI Y+  +T++ K+  A++  + GYF
Sbjct: 334 TGSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYF 361

BLAST of Tan0018335 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 4.2e-40
Identity = 107/344 (31.10%), Postives = 178/344 (51.74%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQFSSTVRL 67
           V + YW A  D     + ID   F+H+ + ++     ++++ I  S       F   +R 
Sbjct: 40  VRSAYWPAGDD--FSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 68  RNPGVKTLLSIAGG---NDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYPSSQ 127
           R+P VKTLLSI GG   + +F+++A+   NR+ FI S+I+VAR   FDG+DL W +P +Q
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 128 DEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATV---SNVPYL-QHNIQ-YPVAAIKQN 187
            +M N   L   W+ A+  +A     P+LLL + V   S V  + +H  + YP  AI + 
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKY 219

Query: 188 LDWVNVLSYDFY----------------TPTFSTHFGIRSWTQSLNNLPHKKIVFGIPFH 247
           LDW + + +D++                    ST+FG+ SW +S   +  +K+V G+  +
Sbjct: 220 LDWASPMCFDYHGTWDNNTDFNAALYDSKSEISTNFGLHSWIKS--GVRPEKLVMGLALY 279

Query: 248 GWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIAY 307
           G AW+L + + + V ++A G A+      +G + Y+ + +F +     NV  D   +  Y
Sbjct: 280 GRAWELKDPNVNGVGAEAVGPAT----DTDGSMNYNEILKF-NKQSGANVVYDKVAISFY 339

Query: 308 TYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADDVWDV 328
           +Y+GTTWI Y+   +I  K+  AKS  + GYF + +  D  W +
Sbjct: 340 SYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKDWSI 374

BLAST of Tan0018335 vs. NCBI nr
Match: XP_022999580.1 (chitotriosidase-1-like [Cucurbita maxima])

HSP 1 Score: 501.5 bits (1290), Expect = 5.4e-138
Identity = 244/341 (71.55%), Postives = 280/341 (82.11%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQF 61
           A +T+FVNAGYWLASTD TLPAANIDATLF+HLF GY  +NS TY+VT+DPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYP 121
           SSTV+L NPGVKTLLSIAG   VFA +A +P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLD 181
           SSQD+M NF+TL+  WH+A+VDDA ++SLPQLLLVATVSN+PYLQ+NIQYP  AI QNLD
Sbjct: 125 SSQDQMANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLD 184

Query: 182 WVNVLSYDFYTP-------------------TFSTHFGIRSWTQSLNNLPHKKIVFGIPF 241
           WVN+LSYDFYTP                     S +FGIRSW QSLNNL HKKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIA 301
           HGWAWKLANSHQH +F+KADGAASGEYI P+GQI+YS+V++FI+DNQA   +KD  +L+A
Sbjct: 245 HGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y Y  TTWI +ESEYT+ADKI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADD 345

BLAST of Tan0018335 vs. NCBI nr
Match: XP_022946077.1 (acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mammalian chitinase-like [Cucurbita moschata])

HSP 1 Score: 497.3 bits (1279), Expect = 1.0e-136
Identity = 243/341 (71.26%), Postives = 278/341 (81.52%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQF 61
           A +T+FVNAGYWLASTD+ LPAANIDATLF+HLF GY  +NS TY+VT+DPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYP 121
           SSTV+L NP VKTLLSIA    VFA +AA+P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLD 181
           SSQD+MDNF+TL+  WH+A+VDDA +LSLPQLLLVATVSN+PYLQ NIQYP  AI Q LD
Sbjct: 125 SSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLD 184

Query: 182 WVNVLSYDFYTP-------------------TFSTHFGIRSWTQSLNNLPHKKIVFGIPF 241
           WVN+LSYDFYTP                     S +FGIRSW QSL+NLPHKKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIA 301
           HGWAWKL NSHQH +F+KADGAA+GEYI P+GQI+YS+V++FI+DNQA   +KD  +L+A
Sbjct: 245 HGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y Y  TTWI YESEYTIADKI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADD 345

BLAST of Tan0018335 vs. NCBI nr
Match: KAG6599362.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1 Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 497.3 bits (1279), Expect = 1.0e-136
Identity = 243/341 (71.26%), Postives = 278/341 (81.52%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQF 61
           A +T+FVNAGYWLASTD+ LPAANIDATLF+HLF GY  +NS TY+VTIDPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILIDQF 64

Query: 62  SSTVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYP 121
           SSTV+L NP VKTLLSIA    +FA +AA+P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLD 181
           SSQD+MDNF+TL+  WH+A+VDDA +LSLPQLLLVATVSN+PYLQ NIQYP  AI Q LD
Sbjct: 125 SSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLD 184

Query: 182 WVNVLSYDFYTP-------------------TFSTHFGIRSWTQSLNNLPHKKIVFGIPF 241
           WVN+LSYDFYTP                     S +FGIRSW QSL+NLPHKKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIA 301
           HGWAWKL NSHQH +F+KADGAA+GEYI P+GQI+YS+V++FI+DNQA   +KD  +L+A
Sbjct: 245 HGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y Y  TTWI YESEYTIADKI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADD 345

BLAST of Tan0018335 vs. NCBI nr
Match: XP_023521369.1 (acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 496.9 bits (1278), Expect = 1.3e-136
Identity = 243/341 (71.26%), Postives = 277/341 (81.23%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQF 61
           A +T+FVNAGYWLASTD+ LPAANIDATLF+HLF GY  +NS TY+VTIDPSQ IL+NQF
Sbjct: 11  ANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQF 70

Query: 62  SSTVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYP 121
           S TVRL NP VKTLLSIA    VFA +AA+P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 71  SPTVRLSNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 130

Query: 122 SSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLD 181
           SSQ+EM NF+TL+  WH+A+VDDA +LSLPQLLLVATVSN+PYLQ NIQYP  AI Q LD
Sbjct: 131 SSQEEMGNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLD 190

Query: 182 WVNVLSYDFYTP-------------------TFSTHFGIRSWTQSLNNLPHKKIVFGIPF 241
           WVN+LSYDFYTP                     S +FGIRSW QSL+NLPHKKIVFGIPF
Sbjct: 191 WVNLLSYDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPF 250

Query: 242 HGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIA 301
           HGWAWKL NSHQH +F+KADGAA+GEYI P+GQI+YS+V++FI+DNQA   +KD  +L+A
Sbjct: 251 HGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 310

Query: 302 YTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           YTY  TTWI YES+YTIADKI+LAK+D VLGYFAFNIAADD
Sbjct: 311 YTYFETTWITYESDYTIADKIALAKADKVLGYFAFNIAADD 351

BLAST of Tan0018335 vs. NCBI nr
Match: XP_022999577.1 (chitotriosidase-1-like [Cucurbita maxima])

HSP 1 Score: 495.7 bits (1275), Expect = 3.0e-136
Identity = 242/341 (70.97%), Postives = 279/341 (81.82%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQF 61
           A +T+FVNAGYWLASTD TLPAANIDATLF+HLF GY  +NS TY+VT+DPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYP 121
           SSTV+L NP VKTLLSIAG   VFA +AA+P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLD 181
           SSQD+M NF+TL+  WH+A+VDDA ++SLPQLLLVATVSN+PYLQ+NIQYP  AI Q LD
Sbjct: 125 SSQDQMANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLD 184

Query: 182 WVNVLSYDFYTP-------------------TFSTHFGIRSWTQSLNNLPHKKIVFGIPF 241
           WVN+LSYDFYTP                     S +FGIRSW QSL+NL HKKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIA 301
           HGWAWKLANSHQH +F+KADGAASGEYI P+GQI+YS+V++FI+DNQA   +KD  +L+A
Sbjct: 245 HGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y Y  TTWI +ESEYT+ADKI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADD 345

BLAST of Tan0018335 vs. ExPASy TrEMBL
Match: A0A6J1KFT3 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.6e-138
Identity = 244/341 (71.55%), Postives = 280/341 (82.11%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQF 61
           A +T+FVNAGYWLASTD TLPAANIDATLF+HLF GY  +NS TY+VT+DPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYP 121
           SSTV+L NPGVKTLLSIAG   VFA +A +P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLD 181
           SSQD+M NF+TL+  WH+A+VDDA ++SLPQLLLVATVSN+PYLQ+NIQYP  AI QNLD
Sbjct: 125 SSQDQMANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLD 184

Query: 182 WVNVLSYDFYTP-------------------TFSTHFGIRSWTQSLNNLPHKKIVFGIPF 241
           WVN+LSYDFYTP                     S +FGIRSW QSLNNL HKKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIA 301
           HGWAWKLANSHQH +F+KADGAASGEYI P+GQI+YS+V++FI+DNQA   +KD  +L+A
Sbjct: 245 HGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y Y  TTWI +ESEYT+ADKI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADD 345

BLAST of Tan0018335 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 5.0e-137
Identity = 243/341 (71.26%), Postives = 278/341 (81.52%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQF 61
           A +T+FVNAGYWLASTD+ LPAANIDATLF+HLF GY  +NS TY+VT+DPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYP 121
           SSTV+L NP VKTLLSIA    VFA +AA+P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLD 181
           SSQD+MDNF+TL+  WH+A+VDDA +LSLPQLLLVATVSN+PYLQ NIQYP  AI Q LD
Sbjct: 125 SSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLD 184

Query: 182 WVNVLSYDFYTP-------------------TFSTHFGIRSWTQSLNNLPHKKIVFGIPF 241
           WVN+LSYDFYTP                     S +FGIRSW QSL+NLPHKKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIA 301
           HGWAWKL NSHQH +F+KADGAA+GEYI P+GQI+YS+V++FI+DNQA   +KD  +L+A
Sbjct: 245 HGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y Y  TTWI YESEYTIADKI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADD 345

BLAST of Tan0018335 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.4e-136
Identity = 242/341 (70.97%), Postives = 279/341 (81.82%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQF 61
           A +T+FVNAGYWLASTD TLPAANIDATLF+HLF GY  +NS TY+VT+DPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYP 121
           SSTV+L NP VKTLLSIAG   VFA +AA+P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLD 181
           SSQD+M NF+TL+  WH+A+VDDA ++SLPQLLLVATVSN+PYLQ+NIQYP  AI Q LD
Sbjct: 125 SSQDQMANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLD 184

Query: 182 WVNVLSYDFYTP-------------------TFSTHFGIRSWTQSLNNLPHKKIVFGIPF 241
           WVN+LSYDFYTP                     S +FGIRSW QSL+NL HKKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIA 301
           HGWAWKLANSHQH +F+KADGAASGEYI P+GQI+YS+V++FI+DNQA   +KD  +L+A
Sbjct: 245 HGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y Y  TTWI +ESEYT+ADKI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADD 345

BLAST of Tan0018335 vs. ExPASy TrEMBL
Match: A0A6J1G4H5 (chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 8.2e-132
Identity = 237/341 (69.50%), Postives = 272/341 (79.77%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQF 61
           A +TKFVNAGYWLAST++ L AANIDATLF+HLFVGY  ++ +T++VT+DPSQ ILINQF
Sbjct: 5   ANVTKFVNAGYWLASTNDRLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQF 64

Query: 62  SSTVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYP 121
           SSTV+L NP VKTLLSIA    VFA +AA P NRD FIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLD 181
           SSQ EM NF+TL+  WH+A+VDDA  +SLPQLLLVATVSN+PYLQ N++YP  AI Q LD
Sbjct: 125 SSQYEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLD 184

Query: 182 WVNVLSYDFYTP-------------------TFSTHFGIRSWTQSLNNLPHKKIVFGIPF 241
           WVN+LSYDFYTP                     S +FGIRSW QSL+NLPHKKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIA 301
           HGWAWKL NSHQH  F+KADGAASGEYI P+GQI+YS+V++FI+DNQA   +KD  +L+A
Sbjct: 245 HGWAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y Y  TTWI YESEYTIADKI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADD 345

BLAST of Tan0018335 vs. ExPASy TrEMBL
Match: A0A6J1G4J9 (acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 6.9e-131
Identity = 235/339 (69.32%), Postives = 270/339 (79.65%), Query Frame = 0

Query: 4   ITKFVNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQFSS 63
           + +FVNAGYWLA TD+TL AANIDATLF+HLFVGY  ++ +T++VT+DPSQ ILINQFSS
Sbjct: 1   MAEFVNAGYWLAGTDDTLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQFSS 60

Query: 64  TVRLRNPGVKTLLSIAGGNDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYPSS 123
           TV+L NP VKTLLSIA    VFA +AA P NRD FIKSSI  AR G FDGLDLQWLYPSS
Sbjct: 61  TVKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYPSS 120

Query: 124 QDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLDWV 183
           Q EM NF+TL+  WH+A+VDDA  +SLPQLLLVATVSN+PYLQ N++YP  AI Q LDWV
Sbjct: 121 QYEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLDWV 180

Query: 184 NVLSYDFYTP-------------------TFSTHFGIRSWTQSLNNLPHKKIVFGIPFHG 243
           N+LSYDFYTP                     S +FGIRSW QSL+NLPHKKIVFGIPFHG
Sbjct: 181 NLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHG 240

Query: 244 WAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIAYT 303
           WAWKL NSHQH  F+KADGAASGEYI P+GQI+YS+V++FI+DNQA   +KD  +L+AY 
Sbjct: 241 WAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 300

Query: 304 YSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y  TTWI YESEYTIADKI+LAK+D VLGYFAFNIAADD
Sbjct: 301 YYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADD 339

BLAST of Tan0018335 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 253.8 bits (647), Expect = 1.8e-67
Identity = 142/339 (41.89%), Postives = 200/339 (59.00%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQFSSTVRL 67
           V A YW  +++   P  +ID++LF+HLF  +  +NSQT +VT+  +     + F+ TV+ 
Sbjct: 28  VKASYWFPASE--FPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 68  RNPGVKTLLSIAGG---NDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYPSSQ 127
           RNP VKTLLSI GG      +A +A+ P +R +FI SSI VAR+  F GLDL W YPSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 128 DEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATV--SNVPYLQHNIQYPVAAIKQNLDW 187
            EM NF TLL  W  A+V +A +   P+LLL A V  SN  Y   ++ YPV+A+  +LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 188 VNVLSYDFYTPTFST------------------HFGIRSWTQSLNNLPHKKIVFGIPFHG 247
           VN+++YDFY P +S                     G RSW Q+   LP KK V G P++G
Sbjct: 208 VNLMAYDFYGPGWSRVTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPYYG 267

Query: 248 WAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIAYT 307
           +AW+L N++ H  ++   GAA    ISP+G I Y  +++FI DN AT V  +   +  Y 
Sbjct: 268 YAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGATTV-YNSTVVGDYC 327

Query: 308 YSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y+GT WI Y+   +I  K+  AK   +LGYF++++ ADD
Sbjct: 328 YAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADD 354

BLAST of Tan0018335 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 242.3 bits (617), Expect = 5.5e-64
Identity = 139/339 (41.00%), Postives = 199/339 (58.70%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQFSSTVRL 67
           V A YW  +TD   PA +ID++LF+HLF  +  + +++YE+TI        + F+ TV+ 
Sbjct: 7   VKASYWFPATD--FPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTETVQQ 66

Query: 68  RNPGVKTLLSIAGGN---DVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYPSSQ 127
           RNP VKTLLSI GGN   D FA +A+ P +R +FI+S+I VAR+  F GLDL W YP ++
Sbjct: 67  RNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYPRNE 126

Query: 128 DEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQ---YPVAAIKQNLD 187
           +EM +F  LL  W  A+  ++ +     L+L A V    Y   N Q   YPV AI  +LD
Sbjct: 127 EEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLAISNSLD 186

Query: 188 WVNVLSYDFYTPTFST-----------------HFGIRSWTQSLNNLPHKKIVFGIPFHG 247
           W+N+++YDFY P +ST                   G+R WT++   LP KK V G P++G
Sbjct: 187 WINLMAYDFYGPGWSTVTGPPASLYLPTDGRSGDSGVRDWTEA--GLPAKKAVLGFPYYG 246

Query: 248 WAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIAYT 307
           WAW LA+    DV +  D   +G  IS +G+I Y  ++ +I DN AT V+ D   +  Y 
Sbjct: 247 WAWTLADP---DV-NGYDANTTGPAISDDGEISYRQLQTWIVDNGATKVH-DDMMVGDYC 306

Query: 308 YSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
           Y+GTTWI Y+SE +I  K+  AK   +LGYF++++  DD
Sbjct: 307 YAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDD 332

BLAST of Tan0018335 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 238.4 bits (607), Expect = 8.0e-63
Identity = 136/337 (40.36%), Postives = 192/337 (56.97%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQAILINQFSSTVRL 67
           V A YW A ++   P A ID++LF+HLF  +  +N+ TY+V +        + F+ TVR 
Sbjct: 26  VKATYWFAESES--PLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVRR 85

Query: 68  RNPGVKTLLSIAGG---NDVFAKVAAEPANRDAFIKSSIDVARAGEFDGLDLQWLYPSSQ 127
           RNP VKTLLSI G    N  FA +A+ P +R  FI SSI +AR+  F GLDL W YPS  
Sbjct: 86  RNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSIT 145

Query: 128 DEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVAAIKQNLDWVN 187
            EMDNF  LL  W  A+  +A +   P+LLL A V    Y  +++ +PV A+  +LDWVN
Sbjct: 146 TEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVNAVADSLDWVN 205

Query: 188 VLSYDFY------------------TPTFSTHFGIRSWTQSLNNLPHKKIVFGIPFHGWA 247
           +++YDFY                  T   S   G+R+WTQ+   LP KK V G P +G+A
Sbjct: 206 LVAYDFYESGSSRVTCSPAPLYDPITTGPSGDAGVRAWTQA--GLPAKKAVLGFPLYGYA 265

Query: 248 WKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQATNVNKDPRFLIAYTYS 307
           W L ++  H+ ++ + G A    ISP+G I Y  +++FI DN+AT V  +   +  Y Y+
Sbjct: 266 WCLTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKATMV-YNSNLVQNYCYA 325

Query: 308 GTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAADD 324
             TWI Y+   +I  K+  AK   +LGYF+++I ADD
Sbjct: 326 KKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADD 352

BLAST of Tan0018335 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 232.6 bits (592), Expect = 4.4e-61
Identity = 137/351 (39.03%), Postives = 194/351 (55.27%), Query Frame = 0

Query: 1   MAQITKF------VNAGYWLASTDETLPAANIDATLFSHLFVGYVGVNSQTYEVTIDPSQ 60
           MA  T+F      V A YW+   +   PA NID+T F+HLF  +  V+S T+EVTI  + 
Sbjct: 1   MADQTQFLRTNSIVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAAN 60

Query: 61  AILINQFSSTVRLRNPGVKTLLSIAGGND----VFAKVAAEPANRDAFIKSSIDVARAGE 120
           +  ++ F+ TV+ +N  V+TLLSI GG D    V A +A+   NR AFI SSID+AR  +
Sbjct: 61  SCQVSSFTHTVKDKNTDVQTLLSI-GGKDADKAVLASMASNSKNRKAFIDSSIDIARKKD 120

Query: 121 FDGLDLQWLYPSSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQ 180
           F GLDL W YPS+  EM NF  L+  W  A+V+++   +   LLL A V   P   +  +
Sbjct: 121 FYGLDLAWEYPSNDVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPD-YYGEE 180

Query: 181 YPVAAIKQNLDWVNVLSYDFYTPTFST-------------------HFGIRSWTQSLNNL 240
           YPV AI  NLD+VN+++YDFY P +S                      G+  W ++   L
Sbjct: 181 YPVQAIADNLDFVNIMAYDFYGPGWSPVTGPPAALFDPSNPAGRSGDSGLSKWLEA--KL 240

Query: 241 PHKKIVFGIPFHGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVKQFIDDNQAT 300
           P KK V G  + GWAW L ++  +   +  DGAA    IS +G I Y+ ++ +I DN A 
Sbjct: 241 PAKKAVLGFSYCGWAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDNGAA 300

Query: 301 NVNKDPRFLIAYTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAAD 323
             + DP  +  Y Y GTTWI Y+   +I  K+  AK   +LGYF++++ AD
Sbjct: 301 TFH-DPAVIGFYCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

BLAST of Tan0018335 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 230.3 bits (586), Expect = 2.2e-60
Identity = 141/360 (39.17%), Postives = 197/360 (54.72%), Query Frame = 0

Query: 1   MAQITKF------VNAGYWL----ASTDETL----PAANIDATLFSHLFVGYVGVNSQTY 60
           MA  T+F      V A YW     + + E L    P++ ID+TLF+HLF  +  V+S T+
Sbjct: 1   MADQTQFLRTNSIVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTH 60

Query: 61  EVTIDPSQAILINQFSSTVRLRNPGVKTLLSIAGGND----VFAKVAAEPANRDAFIKSS 120
           EVTI  + +   + F+ TV+ +N  V+TLLSI GG D    V A +A+   NR AFI SS
Sbjct: 61  EVTISAANSYQFSSFTETVKEKNTDVQTLLSI-GGKDADKAVLASMASNSKNRKAFIDSS 120

Query: 121 IDVARAGEFDGLDLQWLYPSSQDEMDNFETLLSFWHKAIVDDAINLSLPQLLLVATVSNV 180
           ID+AR  +F GLDL W YPS+  EM NF  LL  W  A+V+++   +   LLL A V   
Sbjct: 121 IDIARKKDFYGLDLAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYS 180

Query: 181 PYLQHNIQYPVAAIKQNLDWVNVLSYDFYTPTFST--------------------HFGIR 240
           P     ++YPV AI  NLD+VN+++YDFY P +S                     + G+R
Sbjct: 181 PQYD-GVEYPVKAIADNLDFVNIMAYDFYGPGWSPVTGPPAALFHDPSNPAGRSGNSGLR 240

Query: 241 SWTQSLNNLPHKKIVFGIPFHGWAWKLANSHQHDVFSKADGAASGEYISPNGQIYYSNVK 300
            W      LP KK V G P+ GWAW L ++  +   +  DGAA    ISP+G I Y+ ++
Sbjct: 241 KWLDEA-KLPPKKAVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIR 300

Query: 301 QFIDDNQATNVNKDPRFLIAYTYSGTTWIAYESEYTIADKISLAKSDNVLGYFAFNIAAD 323
            +I DN A   + DP  +  Y Y G TWI Y+   +I  K+  AK   +LGYF++++ AD
Sbjct: 301 NYIVDNGAATFH-DPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGAD 352

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818622.6e-6641.89Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1[more]
A0A072UR651.5e-4534.12Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1[more]
A0A1B1J8Z24.0e-4333.53Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2[more]
U5N4E38.4e-4132.34Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2[more]
A0A072VEP04.2e-4031.10Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022999580.15.4e-13871.55chitotriosidase-1-like [Cucurbita maxima][more]
XP_022946077.11.0e-13671.26acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mamm... [more]
KAG6599362.11.0e-13671.26Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1... [more]
XP_023521369.11.3e-13671.26acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo][more]
XP_022999577.13.0e-13670.97chitotriosidase-1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1KFT32.6e-13871.55chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1[more]
A0A6J1G2P45.0e-13771.26acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE... [more]
A0A6J1KB821.4e-13670.97chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1[more]
A0A6J1G4H58.2e-13269.50chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 ... [more]
A0A6J1G4J96.9e-13169.32acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G19810.11.8e-6741.89Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19800.15.5e-6441.00Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19820.18.0e-6340.36Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19750.14.4e-6139.03Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19760.12.2e-6039.17Glycosyl hydrolase family protein with chitinase insertion domain [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011583Chitinase IISMARTSM006362g34coord: 7..322
e-value: 7.8E-55
score: 198.2
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 24..323
e-value: 1.8E-68
score: 233.4
NoneNo IPR availablePANTHERPTHR11177CHITINASEcoord: 5..324
NoneNo IPR availablePANTHERPTHR11177:SF351CHITINASE-LIKE PROTEIN-RELATEDcoord: 5..324
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 10..322
e-value: 7.7E-53
score: 180.2
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 7..327
score: 49.084518
IPR029070Chitinase insertion domain superfamilyGENE3D3.10.50.10coord: 222..293
e-value: 1.8E-68
score: 233.4
IPR029070Chitinase insertion domain superfamilySUPERFAMILY54556Chitinase insertion domaincoord: 224..293
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 10..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0018335.1Tan0018335.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0008061 chitin binding
molecular_function GO:0016787 hydrolase activity