Tan0018327 (gene) Snake gourd v1

Overview


Name	Tan0018327
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	galactolipase DONGLE, chloroplastic-like
Location	LG10: 12427477 .. 12429197 (+)
RNA-Seq Expression	Tan0018327
Synteny	Tan0018327

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGCGCAACTTTGAAGCTCAATCCAATTCCCAATCATGTCACATTTTCTGAAACAACTCGACCCATGTCTCATTCTTTTGGACAAGTCTCTCTCCCGAGAAGATCCGATAATGTTTCATCGTCGTCATCGTCGTCGTCACGACGGTCGGTTTCTTCTTCTTCGGTTTTAGTACCGTTAATTGATGTGTTTTCTTCTTCTACCGTCGCTGCCTCCGCCGCTGCCATGGTTCACAAGGTGGCCGGTTTGGCGCGGTTGTGGCGGCAGATTCATGGCTGCAATAACTGGGAAGATCTTGTGGAGCCTACACTTCACCCGCTTTTGCGTCGGGAAGTTATCCGGTACGGCGAGTTTGTGACGGCTTGTTATAAAGCTTTTGATCTTGATCCCAACTCAAAACGGTATTTGACCTGCAAATTTGGGAAGAAGAGTTTGTTGAAGGAAGTTGGATTGGAGAGTTCGGGTTATGAAGTGACTAAATACATCTATGCTACCCCACCGGATATCAACATTCCTCCCATCCAAAACTCCCCGCCGTCTTGTGGTCGGTGGATCGGCTACGTCGCTGTCTCTTCCGACGACACTAGTAAGAGACTTGGCCGACGTGACATCGTCATCACTTTCCGAGGTACCGTCACCAACCCCGAATGGATTGCCAATCTAATGAGCTCCCTCACGCCTGCCCGCCTCGACCCTCACAATCATCGACCTGATGTCAAGGTTTGTTTATCTAACCCTCAACTCAAAAGCTTAAATTTCTATTGATTTGCCTTCTAATTTAACCCCAAGACCCAATAAGTAACTATTTACTCTTAATTTGGAGAGAGAAAAAAGAATTGGAACTTTGAATTTTCGAGGAGATGATTTCCAATCAATTAGACCTTCCTCACCCTCCTCCATGAACTCCAATAAGTGAAAATAAAAATATTAGACATCTGGCTCTGATACTATGTTAAAATTCAAAAAATACAACTTTAAAACATGATGACTGACGTTAAAATTTTCACTTTTTCCAGGTCGAATCAGGGTTCCTAACGCTCTACACATGTGAGGAAACGAGCACGAAGTTCGGCCTCGAGAGCTGCCGGGAGCAGCTGCTCTCAGAAGTGTCAAGGCTATTAAACAAGTACAAAAATGAAGAAGTAAGCATAACCATGGCAGGGCACAGCATGGGGAGTGCATTAGCTCTTCTTCTAGCTTATGACATAGCTGAGTTAGGATTGAACAGAAGAACAAACAATGAAGTGGTTCCAGTTTCAGTGTTTTCCTTTGGAGGTCCAAGAGTAGGAAACTCAGGCTTCAAACAAAGATGTGAAGAATTGGGAGTCAAAGTGTTGAGAATTGTGAATGTGAATGATCCAATTACAAAGATGCCTGGTGTTTTATTCAATGAGAATTTTAGGGTTCTTGGAGGTCTCTTAAATGGAGGTGGAAATAATAGTTATGAACATGTTGGAGTTGAATTGCTTCTTGATTTCTTCAACATGCAAAACCCTTCTTGTGTTCATGATTTGGAGACTTACATAAGCTTGCTAAGATCTCCAAAAGATCAACATCAAGATCAAGATCAAGATGATGATGATGATGGGAGAAGAAAGTTTGTTAATGGTGGGGATTTTATCAACAAAGCTATGGAGTTTTTGTGTAGTAATGCTCAGAGCTTGAACATGTTTCCATGGAGGAATCCAGTGAATTACTTGAGTCAATCACAAAATTAG

mRNA sequence

ATGGCGGGCGCAACTTTGAAGCTCAATCCAATTCCCAATCATGTCACATTTTCTGAAACAACTCGACCCATGTCTCATTCTTTTGGACAAGTCTCTCTCCCGAGAAGATCCGATAATGTTTCATCGTCGTCATCGTCGTCGTCACGACGGTCGGTTTCTTCTTCTTCGGTTTTAGTACCGTTAATTGATGTGTTTTCTTCTTCTACCGTCGCTGCCTCCGCCGCTGCCATGGTTCACAAGGTGGCCGGTTTGGCGCGGTTGTGGCGGCAGATTCATGGCTGCAATAACTGGGAAGATCTTGTGGAGCCTACACTTCACCCGCTTTTGCGTCGGGAAGTTATCCGGTACGGCGAGTTTGTGACGGCTTGTTATAAAGCTTTTGATCTTGATCCCAACTCAAAACGGTATTTGACCTGCAAATTTGGGAAGAAGAGTTTGTTGAAGGAAGTTGGATTGGAGAGTTCGGGTTATGAAGTGACTAAATACATCTATGCTACCCCACCGGATATCAACATTCCTCCCATCCAAAACTCCCCGCCGTCTTGTGGTCGGTGGATCGGCTACGTCGCTGTCTCTTCCGACGACACTAGTAAGAGACTTGGCCGACGTGACATCGTCATCACTTTCCGAGGTACCGTCACCAACCCCGAATGGATTGCCAATCTAATGAGCTCCCTCACGCCTGCCCGCCTCGACCCTCACAATCATCGACCTGATGTCAAGGTCGAATCAGGGTTCCTAACGCTCTACACATGTGAGGAAACGAGCACGAAGTTCGGCCTCGAGAGCTGCCGGGAGCAGCTGCTCTCAGAAGTGTCAAGGCTATTAAACAAGTACAAAAATGAAGAAGTAAGCATAACCATGGCAGGGCACAGCATGGGGAGTGCATTAGCTCTTCTTCTAGCTTATGACATAGCTGAGTTAGGATTGAACAGAAGAACAAACAATGAAGTGGTTCCAGTTTCAGTGTTTTCCTTTGGAGGTCCAAGAGTAGGAAACTCAGGCTTCAAACAAAGATGTGAAGAATTGGGAGTCAAAGTGTTGAGAATTGTGAATGTGAATGATCCAATTACAAAGATGCCTGGTGTTTTATTCAATGAGAATTTTAGGGTTCTTGGAGGTCTCTTAAATGGAGGTGGAAATAATAGTTATGAACATGTTGGAGTTGAATTGCTTCTTGATTTCTTCAACATGCAAAACCCTTCTTGTGTTCATGATTTGGAGACTTACATAAGCTTGCTAAGATCTCCAAAAGATCAACATCAAGATCAAGATCAAGATGATGATGATGATGGGAGAAGAAAGTTTGTTAATGGTGGGGATTTTATCAACAAAGCTATGGAGTTTTTGTGTAGTAATGCTCAGAGCTTGAACATGTTTCCATGGAGGAATCCAGTGAATTACTTGAGTCAATCACAAAATTAG

Coding sequence (CDS)

ATGGCGGGCGCAACTTTGAAGCTCAATCCAATTCCCAATCATGTCACATTTTCTGAAACAACTCGACCCATGTCTCATTCTTTTGGACAAGTCTCTCTCCCGAGAAGATCCGATAATGTTTCATCGTCGTCATCGTCGTCGTCACGACGGTCGGTTTCTTCTTCTTCGGTTTTAGTACCGTTAATTGATGTGTTTTCTTCTTCTACCGTCGCTGCCTCCGCCGCTGCCATGGTTCACAAGGTGGCCGGTTTGGCGCGGTTGTGGCGGCAGATTCATGGCTGCAATAACTGGGAAGATCTTGTGGAGCCTACACTTCACCCGCTTTTGCGTCGGGAAGTTATCCGGTACGGCGAGTTTGTGACGGCTTGTTATAAAGCTTTTGATCTTGATCCCAACTCAAAACGGTATTTGACCTGCAAATTTGGGAAGAAGAGTTTGTTGAAGGAAGTTGGATTGGAGAGTTCGGGTTATGAAGTGACTAAATACATCTATGCTACCCCACCGGATATCAACATTCCTCCCATCCAAAACTCCCCGCCGTCTTGTGGTCGGTGGATCGGCTACGTCGCTGTCTCTTCCGACGACACTAGTAAGAGACTTGGCCGACGTGACATCGTCATCACTTTCCGAGGTACCGTCACCAACCCCGAATGGATTGCCAATCTAATGAGCTCCCTCACGCCTGCCCGCCTCGACCCTCACAATCATCGACCTGATGTCAAGGTCGAATCAGGGTTCCTAACGCTCTACACATGTGAGGAAACGAGCACGAAGTTCGGCCTCGAGAGCTGCCGGGAGCAGCTGCTCTCAGAAGTGTCAAGGCTATTAAACAAGTACAAAAATGAAGAAGTAAGCATAACCATGGCAGGGCACAGCATGGGGAGTGCATTAGCTCTTCTTCTAGCTTATGACATAGCTGAGTTAGGATTGAACAGAAGAACAAACAATGAAGTGGTTCCAGTTTCAGTGTTTTCCTTTGGAGGTCCAAGAGTAGGAAACTCAGGCTTCAAACAAAGATGTGAAGAATTGGGAGTCAAAGTGTTGAGAATTGTGAATGTGAATGATCCAATTACAAAGATGCCTGGTGTTTTATTCAATGAGAATTTTAGGGTTCTTGGAGGTCTCTTAAATGGAGGTGGAAATAATAGTTATGAACATGTTGGAGTTGAATTGCTTCTTGATTTCTTCAACATGCAAAACCCTTCTTGTGTTCATGATTTGGAGACTTACATAAGCTTGCTAAGATCTCCAAAAGATCAACATCAAGATCAAGATCAAGATGATGATGATGATGGGAGAAGAAAGTTTGTTAATGGTGGGGATTTTATCAACAAAGCTATGGAGTTTTTGTGTAGTAATGCTCAGAGCTTGAACATGTTTCCATGGAGGAATCCAGTGAATTACTTGAGTCAATCACAAAATTAG

Protein sequence

MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSDNVSSSSSSSSRRSVSSSSVLVPLIDVFSSSTVAASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHDLETYISLLRSPKDQHQDQDQDDDDDGRRKFVNGGDFINKAMEFLCSNAQSLNMFPWRNPVNYLSQSQN

Homology

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BLAST of Tan0018327 vs. ExPASy Swiss-Prot
Match: Q9SIN9 (Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL5 PE=1 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 5.5e-142
Identity = 278/488 (56.97%), Postives = 338/488 (69.26%), Query Frame = 0


Query: 9   NPIPNHVTFSETTRPMSHSF--GQVSLPRRSDNVSSSSSSSSRRSVSSSSVLVPLIDVFS 68
           NP   H  F     P    F    +SLP    N     +++S    SSSS+L P+I    
Sbjct: 6   NPNMKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSS---SSSSLLAPVI---L 65

Query: 69  SSTVAASAAAMVH-----------KVAGLARLWRQIHGCNNWEDLVEPTLHPLLRREVIR 128
           +S VA+S+   ++               L+R+WR+I GCNNW+DL+EP L+PLL++E+ R
Sbjct: 66  NSPVASSSPPPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDLIEP-LNPLLQQEITR 125

Query: 129 YGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLES-SGYEVTKYIYATPP-DINIP 188
           YG  V+ CYKAFDLDPNSKRYL CK+GK++LLKE  ++    Y+VTKYIYATP  +INI 
Sbjct: 126 YGNLVSTCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININIS 185

Query: 189 PIQNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPARLDP 248
           PIQN      RW+GYVA SSDD+ KRLGRRDIV+TFRGTVTNPEW+AN MSSLTPAR  P
Sbjct: 186 PIQNEMNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHP 245

Query: 249 HNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSM 308
           HN R DVKVESGFL+LYT +E+ +KFGLESCR+QLLSE+SRL+NKYK EE+SIT+AGHSM
Sbjct: 246 HNPRLDVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSM 305

Query: 309 GSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNV 368
           GS+LA LLAYDIAELGLNRR     +PV+VFSF GPRVGN  FK+RCEELGVKVLRI NV
Sbjct: 306 GSSLAQLLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNV 365

Query: 369 NDPITKMPGVLFNENFRVLGGLLNGGGNNS-YEHVGVELLLDFFNMQNPSCVHDLETYIS 428
           NDP+TK+PGVLFNENFRVLGG      + S Y HVGVEL LDFF++QN SCVHDL+TYI 
Sbjct: 366 NDPVTKLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYID 425

Query: 429 LLRSPKDQHQDQDQDDDDDGRRKFVNGGDFINKAMEFLCSNAQSL------NMFPWRNPV 475
           LL   +   +  D D+D+D            N A+EFL +N + +       M  W N V
Sbjct: 426 LLNQRRTNSRSVDSDEDEDSD----------NVALEFLKTNGEKMMFLKRQRMMYWSNAV 476

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BLAST of Tan0018327 vs. ExPASy Swiss-Prot
Match: Q9MA46 (Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGL PE=1 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.0e-140
Identity = 266/457 (58.21%), Postives = 334/457 (73.09%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSDNVSSSSSSSSRRSVSSSSVLVP 60
           MA      NPI +     + T    H+    S+ + +      SSS+    +SSS + +P
Sbjct: 1   MAAKVFTQNPIYSQSLVRDKTPQQKHNLDHFSISQHTSKRLVVSSSTMSPPISSSPLSLP 60

Query: 61  LIDVFSSSTVAASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLLRREVIRYGEFV 120
                SSS+ A   +        L+R+WR+I G NNWE+L+EP L P+L++E+ RYG  +
Sbjct: 61  ----SSSSSQAIPPSRAPAVTLPLSRVWREIQGSNNWENLIEP-LSPILQQEITRYGNLL 120

Query: 121 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGL-ESSGYEVTKYIYATPPDINIPPIQNSP 180
           +A YK FDL+PNSKRYL+CK+GKK+LLKE G+ +  GY+VTKYIYAT PDIN+ PI+N  
Sbjct: 121 SASYKGFDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYIYAT-PDINLNPIKNE- 180

Query: 181 PSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPARLDPHNHRPD 240
           P+  RWIGYVAVSSD++ KRLGRRDI++TFRGTVTN EW+ANL SSLTPARLDPHN RPD
Sbjct: 181 PNRARWIGYVAVSSDESVKRLGRRDILVTFRGTVTNHEWLANLKSSLTPARLDPHNPRPD 240

Query: 241 VKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALAL 300
           VKVESGFL LYT  E+ +KFGLESCREQLLSE+SRL+NK+K EE+SIT+AGHSMGS+LA 
Sbjct: 241 VKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKHKGEEISITLAGHSMGSSLAQ 300

Query: 301 LLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITK 360
           LLAYDIAELG+N+R + + VPV+VFSF GPRVGN GFK+RCEELGVKVLRI NVNDPITK
Sbjct: 301 LLAYDIAELGMNQRRDEKPVPVTVFSFAGPRVGNLGFKKRCEELGVKVLRITNVNDPITK 360

Query: 361 MPGVLFNENFRVLGGLLNGGGNNS-YEHVGVELLLDFFNMQNPSCVHDLETYISLLRSPK 420
           +PG LFNENFR LGG+     + S Y HVGVEL LDFF++QN SCVHDLETYI+L+  P+
Sbjct: 361 LPGFLFNENFRSLGGVYELPWSCSCYTHVGVELTLDFFDVQNISCVHDLETYITLVNRPR 420

Query: 421 DQHQDQDQDDDDDGRRKFVNGGDFINKAMEFLCSNAQ 456
                 ++D+          GG+F+N+  E + S  +
Sbjct: 421 CSKLAVNEDN---------FGGEFLNRTSELMFSKGR 441

[Close]

BLAST of Tan0018327 vs. ExPASy Swiss-Prot
Match: A2WY00 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=EG1 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 6.3e-114
Identity = 219/418 (52.39%), Postives = 284/418 (67.94%), Query Frame = 0


Query: 50  RSVSSSSVLVPLIDVFSSSTVAASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLL 109
           R+V+++   V + D   +      AA    +   +A +WR++ GC +WE ++EP  HP+L
Sbjct: 24  RAVAAAGGAVAVRDAVVAPVARRGAARKTAET--VAGMWREVQGCGDWEGMLEPAPHPVL 83

Query: 110 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 169
           R EV RYGE V ACYKAFDLDP S+RYL CK+G++ +L+EVG+  +GYEVT+YIYA   D
Sbjct: 84  RGEVARYGELVGACYKAFDLDPASRRYLNCKYGRERMLEEVGMGGAGYEVTRYIYAA-AD 143

Query: 170 INIPPIQNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPA 229
           +++P ++ S    GRWIGYVAVS+D+ S+RLGRRD++++FRGTVT  EW+ANLMSSL  A
Sbjct: 144 VSVPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAA 203

Query: 230 RLDPHNHRPDVKVESGFLTLYTCEETSTKF-GLESCREQLLSEVSRLLNKYK--NEEVSI 289
           RLDP + RPDVKVESGFL+LYT  + + +F G  SCREQLL EVSRL+  Y    E+VS+
Sbjct: 204 RLDPCDPRPDVKVESGFLSLYTSADKTCRFGGAGSCREQLLREVSRLVAAYSGGGEDVSV 263

Query: 290 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVK 349
           T+AGHSMGSALALL AYD+AELGLNR       PV+VFSFGGPRVGN+ FK RC+ELGVK
Sbjct: 264 TLAGHSMGSALALLSAYDLAELGLNR-----AAPVTVFSFGGPRVGNAAFKARCDELGVK 323

Query: 350 VLRIVNVNDPITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHD 409
            LR+ NV+DPITK+PGV  NE      G+L    ++ Y HVGVEL LDFF + + + VHD
Sbjct: 324 ALRVTNVHDPITKLPGVFLNE---ATAGVLRPWRHSCYTHVGVELPLDFFKVGDLASVHD 383

Query: 410 LETYISLLRSPKDQHQDQDQDDDDDGRRKFVNGGDFINKAMEFLCSNAQSLNMFPWRN 465
           L TYISLLR            D         + G  + K M+F+    +     PW +
Sbjct: 384 LATYISLLRGA----------DKKQPAAAAADAGGVLAKVMDFV-GRRRGGGALPWHD 419

[Close]

BLAST of Tan0018327 vs. ExPASy Swiss-Prot
Match: Q8S1D9 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=EG1 PE=1 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 6.3e-114
Identity = 219/418 (52.39%), Postives = 284/418 (67.94%), Query Frame = 0


Query: 50  RSVSSSSVLVPLIDVFSSSTVAASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLL 109
           R+V+++   V + D   +      AA    +   +A +WR++ GC +WE ++EP  HP+L
Sbjct: 24  RAVAAAGGAVAVRDAVVAPVARRGAARKTAET--VAGMWREVQGCGDWEGMLEPAPHPVL 83

Query: 110 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 169
           R EV RYGE V ACYKAFDLDP S+RYL CK+G++ +L+EVG+  +GYEVT+YIYA   D
Sbjct: 84  RGEVARYGELVGACYKAFDLDPASRRYLNCKYGRERMLEEVGMGGAGYEVTRYIYAA-AD 143

Query: 170 INIPPIQNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPA 229
           +++P ++ S    GRWIGYVAVS+D+ S+RLGRRD++++FRGTVT  EW+ANLMSSL  A
Sbjct: 144 VSVPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAA 203

Query: 230 RLDPHNHRPDVKVESGFLTLYTCEETSTKF-GLESCREQLLSEVSRLLNKYK--NEEVSI 289
           RLDP + RPDVKVESGFL+LYT  + + +F G  SCREQLL EVSRL+  Y    E+VS+
Sbjct: 204 RLDPCDPRPDVKVESGFLSLYTSADKTCRFGGAGSCREQLLREVSRLVAAYSGGGEDVSV 263

Query: 290 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVK 349
           T+AGHSMGSALALL AYD+AELGLNR       PV+VFSFGGPRVGN+ FK RC+ELGVK
Sbjct: 264 TLAGHSMGSALALLSAYDLAELGLNR-----AAPVTVFSFGGPRVGNAAFKARCDELGVK 323

Query: 350 VLRIVNVNDPITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHD 409
            LR+ NV+DPITK+PGV  NE      G+L    ++ Y HVGVEL LDFF + + + VHD
Sbjct: 324 ALRVTNVHDPITKLPGVFLNE---ATAGVLRPWRHSCYTHVGVELPLDFFKVGDLASVHD 383

Query: 410 LETYISLLRSPKDQHQDQDQDDDDDGRRKFVNGGDFINKAMEFLCSNAQSLNMFPWRN 465
           L TYISLLR            D         + G  + K M+F+    +     PW +
Sbjct: 384 LATYISLLRGA----------DKKQPAAAAADAGGVLAKVMDFV-GRRRGGGALPWHD 419

[Close]

BLAST of Tan0018327 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 288.1 bits (736), Expect = 1.8e-76
Identity = 175/400 (43.75%), Postives = 239/400 (59.75%), Query Frame = 0


Query: 88  WRQIHGCNNWEDLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 147
           WR+I G ++W  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +    
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 148 KEVGLESSGYEVTKYIYATPPDINIPPIQNSP------PSCGRWIGYVAVSSDDTSK-RL 207
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS D+TS+ RL
Sbjct: 165 DSLGMIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 208 GRRDIVITFRGTVTNPEWIANLMSSLTPARLDP-HNHRPDVKVESGFLTLYTCEETSTKF 267
           GRRDI I +RGTVT  EWIA+L   L P   +      P VKVESGFL LYT ++T+ KF
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKF 284

Query: 268 GLESCREQLLSEVSRLLNKYKNE---EVSITMAGHSMGSALALLLAYDIAELGLNRRTNN 327
              S REQ+L+EV RL+ ++ ++   ++SIT+ GHS+G ALA+L AYDIAE+ LNR    
Sbjct: 285 ARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKG 344

Query: 328 EVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVLGGLL 387
           +V+PV+V ++GGPRVGN  F++R EELGVKV+R+VNV+D + K PG+  NE+       +
Sbjct: 345 KVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKI 404

Query: 388 NGGGNNSYEHVGVELLLDFFNMQ------NPSCVHDLETYISLLRSPKDQHQDQDQDDDD 447
             G    Y HVG EL LD  N        + S  H+LE  + LL    D +  +      
Sbjct: 405 AEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL----DGYHGK------ 464

Query: 448 DGRRKFVNGG---DFINKAMEFLCSNAQSLNMFPWRNPVN 468
            G R  ++ G     +NKA +FL  + Q +  F WR   N
Sbjct: 465 -GERFVLSSGRDHALVNKASDFLKEHLQ-IPPF-WRQDAN 489

[Close]

BLAST of Tan0018327 vs. NCBI nr
Match: XP_004148168.2 (galactolipase DONGLE, chloroplastic [Cucumis sativus] >KGN57254.1 hypothetical protein Csa_009777 [Cucumis sativus])

HSP 1 Score: 852.4 bits (2201), Expect = 1.8e-243
Identity = 431/486 (88.68%), Postives = 451/486 (92.80%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSD-NVSSSSSSSS------RRSVS 60
           MA  TLKLN IPNHVTF ETTRPMSHSFGQVS+PR+SD NVSSSSSSSS      R SVS
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  SSSVLVPLIDVFSSSTVA----ASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLL 120
           SSSVLVPL+DVFSSS+ +    A+AAAMV KVA LARLWRQIHGCN+WEDLVEPTLHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPA 240
           INIPPIQNSPPSCGRWIGYVAVSSD+TSKRLGRRDI+ITFRGTVTNPEWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYT EE+S KFGLESCREQLLSEVSRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHDLET 420
           IVNVNDPITKMPGVLFNENFRV GGLLNGG NNSYEHVGVEL+LDFFNMQNPSCVHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRSPKDQHQDQDQDDDDDGRRK-FVNGGDFINKAMEFLCSNAQSLNMFPWRNPVNY 475
           YISLLR PK Q  + + +D+D GR K  +N G+FINKAMEFLCSNAQSLNMFPWRNP+NY
Sbjct: 421 YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNY 480

[Close]

BLAST of Tan0018327 vs. NCBI nr
Match: XP_008439078.1 (PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis melo] >KAA0067660.1 galactolipase DONGLE [Cucumis melo var. makuwa] >TYK23664.1 galactolipase DONGLE [Cucumis melo var. makuwa])

HSP 1 Score: 840.9 bits (2171), Expect = 5.5e-240
Identity = 428/492 (86.99%), Postives = 451/492 (91.67%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSD-NVSSSSSSSS-------RRSV 60
           MA  TLKLN IPNHVTF ETTRPMSHSFGQVS+PR+SD NVSSSSSSSS       R SV
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  SSSSVLVPLIDVFSSSTVA------ASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLH 120
           SSSSVLVPL+DVFSSS+ +      A+AAAMV KVA LARLWRQIHGCN+WEDLVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSL 240
           PPDINIPPIQNSPPSCGRWIGYVAVSSD+TSKRLGRRDI+ITFRGTVT+PEWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYT EE++ KFGLESCREQLLSEVSR+LNKYK EEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFR  GGLLNGG NNSYEHVGVEL+LDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRSPK---DQHQDQDQDDDDDGRRKF-VNGGDFINKAMEFLCSNAQSLNMFPW 475
           LETYISLLR PK   D+ QD+D   D +GRRK  +N G+FINKA EFLCSNAQSLNMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

[Close]

BLAST of Tan0018327 vs. NCBI nr
Match: XP_038886126.1 (galactolipase DONGLE, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 839.7 bits (2168), Expect = 1.2e-239
Identity = 424/478 (88.70%), Postives = 442/478 (92.47%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSDNVSSSSSSSSRRSVSSSSVLVP 60
           MA  TLKLN IPNHVTF ETTRPMSHSFGQV +P + +NV S SSSS R SVSSS VLVP
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVLIPIKPNNVLSLSSSSRRLSVSSSLVLVP 60

Query: 61  LIDVFSSSTVAASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLLRREVIRYGEFV 120
           L+DVFSSS   A+AAAMVHKVA LA LWRQIHGCN+WEDL+EPTLHPLLRRE+IRYGEFV
Sbjct: 61  LMDVFSSSCSTAAAAAMVHKVASLAHLWRQIHGCNDWEDLMEPTLHPLLRREIIRYGEFV 120

Query: 121 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPP 180
           TACYKAFDLDPNSKRYLTCKFGKK+LL+EVGLESSGYEVTKYIYATPPDINI PIQNSPP
Sbjct: 121 TACYKAFDLDPNSKRYLTCKFGKKNLLREVGLESSGYEVTKYIYATPPDINISPIQNSPP 180

Query: 181 SCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPARLDPHNHRPDV 240
           SCGRWIGYVAVSSD+TSKRLGRRDIVITFRGTVTNPEWIANLMSSLT ARLDPHNHRPDV
Sbjct: 181 SCGRWIGYVAVSSDETSKRLGRRDIVITFRGTVTNPEWIANLMSSLTLARLDPHNHRPDV 240

Query: 241 KVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALALL 300
           KVESGFLTLYT EETS KFGLESCREQLLSEVSRLLNKYK EEVSITMAGHSMGSALALL
Sbjct: 241 KVESGFLTLYTSEETSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMAGHSMGSALALL 300

Query: 301 LAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKM 360
           LAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RCEELGVKVLRIVNVNDPITKM
Sbjct: 301 LAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLRIVNVNDPITKM 360

Query: 361 PGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHDLETYISLLR-SPK- 420
           PGVLFNENFRV GGLLNGGGNNSYEHVGVEL+LDFFNMQNPSCVHDLETYISLLR  PK 
Sbjct: 361 PGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLRCDPKQ 420

Query: 421 -DQHQDQDQDDDDDGRRK-FVNGGDFINKAMEFLCSNAQSLNMFPWRNPVNYLSQSQN 475
            + HQD D DD ++GRRK  +N G+FINKAMEFLCSNAQ LNMFPWR PVNYLSQSQN
Sbjct: 421 EEDHQDNDDDDHNNGRRKIIINSGEFINKAMEFLCSNAQILNMFPWRIPVNYLSQSQN 478

[Close]

BLAST of Tan0018327 vs. NCBI nr
Match: XP_022979567.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 786.2 bits (2029), Expect = 1.6e-223
Identity = 404/475 (85.05%), Postives = 425/475 (89.47%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSDNVSSSSSSSSRRSVSSSSVLVP 60
           MA  TLKLN   NHVTFSETTRPMSHSFGQVSLPR+SD+VSS+S  S   S SSSSVLVP
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPMSHSFGQVSLPRKSDHVSSTSRRSVSSS-SSSSVLVP 60

Query: 61  LIDVFSSSTVAASAA-AMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLLRREVIRYGEF 120
           LID+FSSS+ +ASAA AMVHK+AGLARLWRQIHGCN+WE LVEPTLHPLLRREVIRYGEF
Sbjct: 61  LIDMFSSSSSSASAAVAMVHKMAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEF 120

Query: 121 VTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSP 180
           VTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSP
Sbjct: 121 VTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSP 180

Query: 181 PSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPARLDPHNHRPD 240
           PSCGRWIGYVAVSSD+TSKRLGRRD+VITFRGTVTN EWIANLMSSLTPARLDPHNHRPD
Sbjct: 181 PSCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPD 240

Query: 241 VKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALAL 300
           VKVESGFLTLYTCEETSTKFGLESCREQLLSE+SRLLNKYK+EEVSITMAGHSMGSALAL
Sbjct: 241 VKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALAL 300

Query: 301 LLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITK 360
           LLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR+VNVNDPITK
Sbjct: 301 LLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITK 360

Query: 361 MPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHDLETYISLLRSPKD 420
           +PGV               GG   YEHVGVEL+LDFFNMQNPSCVHDLETYISLLR PK+
Sbjct: 361 IPGVF--------------GG---YEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKE 420

Query: 421 QHQDQDQDDDDDGRRKFVNGGDFINKAMEFLCSNAQSLNMFPWRNPVNYLSQSQN 475
           +HQ  D D   +GRRK +NGGDFINKAMEFL     S NM PWRNP+NYLSQSQN
Sbjct: 421 EHQKHDDDHHHNGRRKVINGGDFINKAMEFL-----SFNMLPWRNPMNYLSQSQN 452

[Close]

BLAST of Tan0018327 vs. NCBI nr
Match: XP_022956251.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 782.3 bits (2019), Expect = 2.3e-222
Identity = 404/481 (83.99%), Postives = 427/481 (88.77%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSDNVSSSSSSSSRRSVSSSSVLVP 60
           MA  TLKLN   NHVTFSETTRP+SHSFGQVSLPR+SD+VSS+S  S   S SSSSVLVP
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSS-SSSSVLVP 60

Query: 61  LIDVFSSSTVAAS-----AAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLLRREVIR 120
           LID+FSSS+ ++S     A AMVHKVAGLARLWRQIHGCN+WE LVEPTLHPLLRREVIR
Sbjct: 61  LIDMFSSSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIR 120

Query: 121 YGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPI 180
           YGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPI
Sbjct: 121 YGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPI 180

Query: 181 QNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPARLDPHN 240
           QNSPPSCGRWIGYVAVSSD+TS+RLGRRD+VITFRGTVTN EWIANLMSSLTPARLDPHN
Sbjct: 181 QNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHN 240

Query: 241 HRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGS 300
           HRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSE+SRLLNKYK+EEVSITMAGHSMGS
Sbjct: 241 HRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGS 300

Query: 301 ALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVND 360
           ALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR+VNVND
Sbjct: 301 ALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVND 360

Query: 361 PITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHDLETYISLLR 420
           PITKMPGV               GG   YEHVGVEL+LDFFNMQNPSCVHDLETYISLLR
Sbjct: 361 PITKMPGVF--------------GG---YEHVGVELVLDFFNMQNPSCVHDLETYISLLR 420

Query: 421 SPKDQHQDQDQDD--DDDGRRKFVNGGDFINKAMEFLCSNAQSLNMFPWRNPVNYLSQSQ 475
            PK++HQ+ D DD    +GRRK +NGGDFINKAMEFL     S NMFPWRNP+NYLSQSQ
Sbjct: 421 YPKEEHQEHDDDDHHHHNGRRKAINGGDFINKAMEFL-----SFNMFPWRNPMNYLSQSQ 458

[Close]

BLAST of Tan0018327 vs. ExPASy TrEMBL
Match: A0A0A0L8A7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174570 PE=4 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.8e-244
Identity = 431/486 (88.68%), Postives = 451/486 (92.80%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSD-NVSSSSSSSS------RRSVS 60
           MA  TLKLN IPNHVTF ETTRPMSHSFGQVS+PR+SD NVSSSSSSSS      R SVS
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  SSSVLVPLIDVFSSSTVA----ASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLL 120
           SSSVLVPL+DVFSSS+ +    A+AAAMV KVA LARLWRQIHGCN+WEDLVEPTLHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPA 240
           INIPPIQNSPPSCGRWIGYVAVSSD+TSKRLGRRDI+ITFRGTVTNPEWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYT EE+S KFGLESCREQLLSEVSRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHDLET 420
           IVNVNDPITKMPGVLFNENFRV GGLLNGG NNSYEHVGVEL+LDFFNMQNPSCVHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRSPKDQHQDQDQDDDDDGRRK-FVNGGDFINKAMEFLCSNAQSLNMFPWRNPVNY 475
           YISLLR PK Q  + + +D+D GR K  +N G+FINKAMEFLCSNAQSLNMFPWRNP+NY
Sbjct: 421 YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNY 480

[Close]

BLAST of Tan0018327 vs. ExPASy TrEMBL
Match: A0A5A7VKE2 (Galactolipase DONGLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00300 PE=4 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.7e-240
Identity = 428/492 (86.99%), Postives = 451/492 (91.67%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSD-NVSSSSSSSS-------RRSV 60
           MA  TLKLN IPNHVTF ETTRPMSHSFGQVS+PR+SD NVSSSSSSSS       R SV
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  SSSSVLVPLIDVFSSSTVA------ASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLH 120
           SSSSVLVPL+DVFSSS+ +      A+AAAMV KVA LARLWRQIHGCN+WEDLVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSL 240
           PPDINIPPIQNSPPSCGRWIGYVAVSSD+TSKRLGRRDI+ITFRGTVT+PEWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYT EE++ KFGLESCREQLLSEVSR+LNKYK EEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFR  GGLLNGG NNSYEHVGVEL+LDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRSPK---DQHQDQDQDDDDDGRRKF-VNGGDFINKAMEFLCSNAQSLNMFPW 475
           LETYISLLR PK   D+ QD+D   D +GRRK  +N G+FINKA EFLCSNAQSLNMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

[Close]

BLAST of Tan0018327 vs. ExPASy TrEMBL
Match: A0A1S3AXV1 (galactolipase DONGLE, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483973 PE=4 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.7e-240
Identity = 428/492 (86.99%), Postives = 451/492 (91.67%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSD-NVSSSSSSSS-------RRSV 60
           MA  TLKLN IPNHVTF ETTRPMSHSFGQVS+PR+SD NVSSSSSSSS       R SV
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  SSSSVLVPLIDVFSSSTVA------ASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLH 120
           SSSSVLVPL+DVFSSS+ +      A+AAAMV KVA LARLWRQIHGCN+WEDLVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSL 240
           PPDINIPPIQNSPPSCGRWIGYVAVSSD+TSKRLGRRDI+ITFRGTVT+PEWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYT EE++ KFGLESCREQLLSEVSR+LNKYK EEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFK+RC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFR  GGLLNGG NNSYEHVGVEL+LDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRSPK---DQHQDQDQDDDDDGRRKF-VNGGDFINKAMEFLCSNAQSLNMFPW 475
           LETYISLLR PK   D+ QD+D   D +GRRK  +N G+FINKA EFLCSNAQSLNMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

[Close]

BLAST of Tan0018327 vs. ExPASy TrEMBL
Match: A0A6J1IP19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479244 PE=4 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 7.7e-224
Identity = 404/475 (85.05%), Postives = 425/475 (89.47%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSDNVSSSSSSSSRRSVSSSSVLVP 60
           MA  TLKLN   NHVTFSETTRPMSHSFGQVSLPR+SD+VSS+S  S   S SSSSVLVP
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPMSHSFGQVSLPRKSDHVSSTSRRSVSSS-SSSSVLVP 60

Query: 61  LIDVFSSSTVAASAA-AMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLLRREVIRYGEF 120
           LID+FSSS+ +ASAA AMVHK+AGLARLWRQIHGCN+WE LVEPTLHPLLRREVIRYGEF
Sbjct: 61  LIDMFSSSSSSASAAVAMVHKMAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGEF 120

Query: 121 VTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSP 180
           VTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSP
Sbjct: 121 VTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSP 180

Query: 181 PSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPARLDPHNHRPD 240
           PSCGRWIGYVAVSSD+TSKRLGRRD+VITFRGTVTN EWIANLMSSLTPARLDPHNHRPD
Sbjct: 181 PSCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRPD 240

Query: 241 VKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALAL 300
           VKVESGFLTLYTCEETSTKFGLESCREQLLSE+SRLLNKYK+EEVSITMAGHSMGSALAL
Sbjct: 241 VKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALAL 300

Query: 301 LLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITK 360
           LLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR+VNVNDPITK
Sbjct: 301 LLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPITK 360

Query: 361 MPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHDLETYISLLRSPKD 420
           +PGV               GG   YEHVGVEL+LDFFNMQNPSCVHDLETYISLLR PK+
Sbjct: 361 IPGVF--------------GG---YEHVGVELVLDFFNMQNPSCVHDLETYISLLRYPKE 420

Query: 421 QHQDQDQDDDDDGRRKFVNGGDFINKAMEFLCSNAQSLNMFPWRNPVNYLSQSQN 475
           +HQ  D D   +GRRK +NGGDFINKAMEFL     S NM PWRNP+NYLSQSQN
Sbjct: 421 EHQKHDDDHHHNGRRKVINGGDFINKAMEFL-----SFNMLPWRNPMNYLSQSQN 452

[Close]

BLAST of Tan0018327 vs. ExPASy TrEMBL
Match: A0A6J1GW19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458003 PE=4 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.1e-222
Identity = 404/481 (83.99%), Postives = 427/481 (88.77%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSDNVSSSSSSSSRRSVSSSSVLVP 60
           MA  TLKLN   NHVTFSETTRP+SHSFGQVSLPR+SD+VSS+S  S   S SSSSVLVP
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSDHVSSTSRRSVSSS-SSSSVLVP 60

Query: 61  LIDVFSSSTVAAS-----AAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLLRREVIR 120
           LID+FSSS+ ++S     A AMVHKVAGLARLWRQIHGCN+WE LVEPTLHPLLRREVIR
Sbjct: 61  LIDMFSSSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIR 120

Query: 121 YGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPI 180
           YGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPI
Sbjct: 121 YGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPI 180

Query: 181 QNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPARLDPHN 240
           QNSPPSCGRWIGYVAVSSD+TS+RLGRRD+VITFRGTVTN EWIANLMSSLTPARLDPHN
Sbjct: 181 QNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHN 240

Query: 241 HRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGS 300
           HRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSE+SRLLNKYK+EEVSITMAGHSMGS
Sbjct: 241 HRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGS 300

Query: 301 ALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVND 360
           ALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR+VNVND
Sbjct: 301 ALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVND 360

Query: 361 PITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQNPSCVHDLETYISLLR 420
           PITKMPGV               GG   YEHVGVEL+LDFFNMQNPSCVHDLETYISLLR
Sbjct: 361 PITKMPGVF--------------GG---YEHVGVELVLDFFNMQNPSCVHDLETYISLLR 420

Query: 421 SPKDQHQDQDQDD--DDDGRRKFVNGGDFINKAMEFLCSNAQSLNMFPWRNPVNYLSQSQ 475
            PK++HQ+ D DD    +GRRK +NGGDFINKAMEFL     S NMFPWRNP+NYLSQSQ
Sbjct: 421 YPKEEHQEHDDDDHHHHNGRRKAINGGDFINKAMEFL-----SFNMFPWRNPMNYLSQSQ 458

[Close]

BLAST of Tan0018327 vs. TAIR 10
Match: AT2G31690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 505.8 bits (1301), Expect = 3.9e-143
Identity = 278/488 (56.97%), Postives = 338/488 (69.26%), Query Frame = 0


Query: 9   NPIPNHVTFSETTRPMSHSF--GQVSLPRRSDNVSSSSSSSSRRSVSSSSVLVPLIDVFS 68
           NP   H  F     P    F    +SLP    N     +++S    SSSS+L P+I    
Sbjct: 6   NPNMKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSS---SSSSLLAPVI---L 65

Query: 69  SSTVAASAAAMVH-----------KVAGLARLWRQIHGCNNWEDLVEPTLHPLLRREVIR 128
           +S VA+S+   ++               L+R+WR+I GCNNW+DL+EP L+PLL++E+ R
Sbjct: 66  NSPVASSSPPPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDLIEP-LNPLLQQEITR 125

Query: 129 YGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLES-SGYEVTKYIYATPP-DINIP 188
           YG  V+ CYKAFDLDPNSKRYL CK+GK++LLKE  ++    Y+VTKYIYATP  +INI 
Sbjct: 126 YGNLVSTCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININIS 185

Query: 189 PIQNSPPSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPARLDP 248
           PIQN      RW+GYVA SSDD+ KRLGRRDIV+TFRGTVTNPEW+AN MSSLTPAR  P
Sbjct: 186 PIQNEMNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHP 245

Query: 249 HNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSM 308
           HN R DVKVESGFL+LYT +E+ +KFGLESCR+QLLSE+SRL+NKYK EE+SIT+AGHSM
Sbjct: 246 HNPRLDVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSM 305

Query: 309 GSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNV 368
           GS+LA LLAYDIAELGLNRR     +PV+VFSF GPRVGN  FK+RCEELGVKVLRI NV
Sbjct: 306 GSSLAQLLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNV 365

Query: 369 NDPITKMPGVLFNENFRVLGGLLNGGGNNS-YEHVGVELLLDFFNMQNPSCVHDLETYIS 428
           NDP+TK+PGVLFNENFRVLGG      + S Y HVGVEL LDFF++QN SCVHDL+TYI 
Sbjct: 366 NDPVTKLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYID 425

Query: 429 LLRSPKDQHQDQDQDDDDDGRRKFVNGGDFINKAMEFLCSNAQSL------NMFPWRNPV 475
           LL   +   +  D D+D+D            N A+EFL +N + +       M  W N V
Sbjct: 426 LLNQRRTNSRSVDSDEDEDSD----------NVALEFLKTNGEKMMFLKRQRMMYWSNAV 476

[Close]

BLAST of Tan0018327 vs. TAIR 10
Match: AT1G05800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 500.0 bits (1286), Expect = 2.1e-141
Identity = 266/457 (58.21%), Postives = 334/457 (73.09%), Query Frame = 0


Query: 1   MAGATLKLNPIPNHVTFSETTRPMSHSFGQVSLPRRSDNVSSSSSSSSRRSVSSSSVLVP 60
           MA      NPI +     + T    H+    S+ + +      SSS+    +SSS + +P
Sbjct: 1   MAAKVFTQNPIYSQSLVRDKTPQQKHNLDHFSISQHTSKRLVVSSSTMSPPISSSPLSLP 60

Query: 61  LIDVFSSSTVAASAAAMVHKVAGLARLWRQIHGCNNWEDLVEPTLHPLLRREVIRYGEFV 120
                SSS+ A   +        L+R+WR+I G NNWE+L+EP L P+L++E+ RYG  +
Sbjct: 61  ----SSSSSQAIPPSRAPAVTLPLSRVWREIQGSNNWENLIEP-LSPILQQEITRYGNLL 120

Query: 121 TACYKAFDLDPNSKRYLTCKFGKKSLLKEVGL-ESSGYEVTKYIYATPPDINIPPIQNSP 180
           +A YK FDL+PNSKRYL+CK+GKK+LLKE G+ +  GY+VTKYIYAT PDIN+ PI+N  
Sbjct: 121 SASYKGFDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYIYAT-PDINLNPIKNE- 180

Query: 181 PSCGRWIGYVAVSSDDTSKRLGRRDIVITFRGTVTNPEWIANLMSSLTPARLDPHNHRPD 240
           P+  RWIGYVAVSSD++ KRLGRRDI++TFRGTVTN EW+ANL SSLTPARLDPHN RPD
Sbjct: 181 PNRARWIGYVAVSSDESVKRLGRRDILVTFRGTVTNHEWLANLKSSLTPARLDPHNPRPD 240

Query: 241 VKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALAL 300
           VKVESGFL LYT  E+ +KFGLESCREQLLSE+SRL+NK+K EE+SIT+AGHSMGS+LA 
Sbjct: 241 VKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKHKGEEISITLAGHSMGSSLAQ 300

Query: 301 LLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITK 360
           LLAYDIAELG+N+R + + VPV+VFSF GPRVGN GFK+RCEELGVKVLRI NVNDPITK
Sbjct: 301 LLAYDIAELGMNQRRDEKPVPVTVFSFAGPRVGNLGFKKRCEELGVKVLRITNVNDPITK 360

Query: 361 MPGVLFNENFRVLGGLLNGGGNNS-YEHVGVELLLDFFNMQNPSCVHDLETYISLLRSPK 420
           +PG LFNENFR LGG+     + S Y HVGVEL LDFF++QN SCVHDLETYI+L+  P+
Sbjct: 361 LPGFLFNENFRSLGGVYELPWSCSCYTHVGVELTLDFFDVQNISCVHDLETYITLVNRPR 420

Query: 421 DQHQDQDQDDDDDGRRKFVNGGDFINKAMEFLCSNAQ 456
                 ++D+          GG+F+N+  E + S  +
Sbjct: 421 CSKLAVNEDN---------FGGEFLNRTSELMFSKGR 441

[Close]

BLAST of Tan0018327 vs. TAIR 10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 1.3e-77
Identity = 175/400 (43.75%), Postives = 239/400 (59.75%), Query Frame = 0


Query: 88  WRQIHGCNNWEDLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 147
           WR+I G ++W  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +    
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 148 KEVGLESSGYEVTKYIYATPPDINIPPIQNSP------PSCGRWIGYVAVSSDDTSK-RL 207
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS D+TS+ RL
Sbjct: 165 DSLGMIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 208 GRRDIVITFRGTVTNPEWIANLMSSLTPARLDP-HNHRPDVKVESGFLTLYTCEETSTKF 267
           GRRDI I +RGTVT  EWIA+L   L P   +      P VKVESGFL LYT ++T+ KF
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKF 284

Query: 268 GLESCREQLLSEVSRLLNKYKNE---EVSITMAGHSMGSALALLLAYDIAELGLNRRTNN 327
              S REQ+L+EV RL+ ++ ++   ++SIT+ GHS+G ALA+L AYDIAE+ LNR    
Sbjct: 285 ARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKG 344

Query: 328 EVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVLGGLL 387
           +V+PV+V ++GGPRVGN  F++R EELGVKV+R+VNV+D + K PG+  NE+       +
Sbjct: 345 KVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKI 404

Query: 388 NGGGNNSYEHVGVELLLDFFNMQ------NPSCVHDLETYISLLRSPKDQHQDQDQDDDD 447
             G    Y HVG EL LD  N        + S  H+LE  + LL    D +  +      
Sbjct: 405 AEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL----DGYHGK------ 464

Query: 448 DGRRKFVNGG---DFINKAMEFLCSNAQSLNMFPWRNPVN 468
            G R  ++ G     +NKA +FL  + Q +  F WR   N
Sbjct: 465 -GERFVLSSGRDHALVNKASDFLKEHLQ-IPPF-WRQDAN 489

[Close]

BLAST of Tan0018327 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.7e-77
Identity = 161/344 (46.80%), Postives = 217/344 (63.08%), Query Frame = 0


Query: 88  WRQIHGCNNWEDLVEPTLHPLLRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 147
           WR+I G ++W  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +    
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 148 KEVGLESSGYEVTKYIYATPPDINIPPIQNSP------PSCGRWIGYVAVSSDDTSK-RL 207
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS D+TS+ RL
Sbjct: 165 DSLGMIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 208 GRRDIVITFRGTVTNPEWIANLMSSLTPARLDP-HNHRPDVKVESGFLTLYTCEETSTKF 267
           GRRDI I +RGTVT  EWIA+L   L P   +      P VKVESGFL LYT ++T+ KF
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKF 284

Query: 268 GLESCREQLLSEVSRLLNKYKNE---EVSITMAGHSMGSALALLLAYDIAELGLNRRTNN 327
              S REQ+L+EV RL+ ++ ++   ++SIT+ GHS+G ALA+L AYDIAE+ LNR    
Sbjct: 285 ARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKG 344

Query: 328 EVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVLGGLL 387
           +V+PV+V ++GGPRVGN  F++R EELGVKV+R+VNV+D + K PG+  NE+       +
Sbjct: 345 KVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKI 404

Query: 388 NGGGNNSYEHVGVELLLDFFNMQ------NPSCVHDLETYISLL 415
             G    Y HVG EL LD  N        + S  H+LE  + LL
Sbjct: 405 AEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL 446

[Close]

BLAST of Tan0018327 vs. TAIR 10
Match: AT1G06800.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 280.8 bits (717), Expect = 2.0e-75
Identity = 178/443 (40.18%), Postives = 249/443 (56.21%), Query Frame = 0


Query: 4   ATLKLNPIPNHVT-----FSETTRPMSHSFGQVSLPRRSDNVSSSSSSSSRRSVSSSSVL 63
           AT+  + +  H T     +S + RP       ++ P RS    + S +    S+S+    
Sbjct: 2   ATIPSHNLRPHTTNQRTQYSLSFRPHFSRSTLITFPARSSPARAMSRTDEEASISTR--- 61

Query: 64  VPLIDVFSSSTVAASAAAMVHKVAGLAR-------LWRQIHGCNNWEDLVEPTLHPLLRR 123
                     +   + A  + +  G A+        WR+I G ++W  L++P + P+LR 
Sbjct: 62  ------LEQESYGLTTAEDIRRRDGEAKESKRLRDTWRKIQGEDDWAGLMDP-MDPVLRS 121

Query: 124 EVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDIN 183
           E+IRYGE   ACY AFD DP S+   +C+F ++ L   +G+  SGYEV +Y+YAT  +IN
Sbjct: 122 ELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFDSLGIIDSGYEVARYLYAT-SNIN 181

Query: 184 IPPIQNSP------PSCGRWIGYVAVSSDD--TSKRLGRRDIVITFRGTVTNPEWIANLM 243
           +P   +             W+GYVAVS D+  T  RLGRRDI I +RGTVT  EWIA+L 
Sbjct: 182 LPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLGRRDIAIAWRGTVTRLEWIADLK 241

Query: 244 SSLTPARLDPHN-HRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEVSRLLNKY--- 303
             L P   +      P VK ESGFL LYT ++TS  F   S REQ+L+EV RL+ +Y   
Sbjct: 242 DFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFSKFSAREQVLTEVKRLVERYGDE 301

Query: 304 KNEEVSITMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKQR 363
           + EE+SIT+ GHS+G ALA+L AYD+AE+G+NR    +V+PV+ F++GGPRVGN  FK+R
Sbjct: 302 EGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGKVIPVTAFTYGGPRVGNIRFKER 361

Query: 364 CEELGVKVLRIVNVNDPITKMPGVLFNENFRVLGGLLNGGGNNSYEHVGVELLLDFFNMQ 415
            E+LGVKVLR+VN +D + K PG+  NE        L GG    Y HVG  L LD  + +
Sbjct: 362 IEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLAGGLPWCYSHVGEMLPLD--HQK 421

The following BLAST results are available for this feature:

BLAST of Tan0018327 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIN9	5.5e-142	56.97	Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL5...	[more]
Q9MA46	3.0e-140	58.21	Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGL PE=1 ...	[more]
A2WY00	6.3e-114	52.39	Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica ...	[more]
Q8S1D9	6.3e-114	52.39	Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonic...	[more]
Q3EBR6	1.8e-76	43.75	Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3...	[more]

BLAST of Tan0018327 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148168.2	1.8e-243	88.68	galactolipase DONGLE, chloroplastic [Cucumis sativus] >KGN57254.1 hypothetical p...	[more]
XP_008439078.1	5.5e-240	86.99	PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis melo] >KAA0067660.1...	[more]
XP_038886126.1	1.2e-239	88.70	galactolipase DONGLE, chloroplastic-like [Benincasa hispida]	[more]
XP_022979567.1	1.6e-223	85.05	phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita maxima]	[more]
XP_022956251.1	2.3e-222	83.99	phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of Tan0018327 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8A7	8.8e-244	88.68	Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174570 PE...	[more]
A0A5A7VKE2	2.7e-240	86.99	Galactolipase DONGLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577...	[more]
A0A1S3AXV1	2.7e-240	86.99	galactolipase DONGLE, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483973...	[more]
A0A6J1IP19	7.7e-224	85.05	phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1GW19	1.1e-222	83.99	phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of Tan0018327 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G31690.1	3.9e-143	56.97	alpha/beta-Hydrolases superfamily protein	[more]
AT1G05800.1	2.1e-141	58.21	alpha/beta-Hydrolases superfamily protein	[more]
AT2G30550.2	1.3e-77	43.75	alpha/beta-Hydrolases superfamily protein	[more]
AT2G30550.1	1.7e-77	46.80	alpha/beta-Hydrolases superfamily protein	[more]
AT1G06800.2	2.0e-75	40.18	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 473..474
None	No IPR available	COILS	Coil	Coil	coord: 265..285
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 416..435
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 418..435
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 15..50
None	No IPR available	PANTHER	PTHR31403:SF4	PHOSPHOLIPASE A1-IALPHA2, CHLOROPLASTIC	coord: 21..464
None	No IPR available	PANTHER	PTHR31403	PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC	coord: 21..464
None	No IPR available	CDD	cd00519	Lipase_3	coord: 150..415 e-value: 1.44452E-49 score: 167.268
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 206..363 e-value: 4.4E-38 score: 130.4
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 87..460 e-value: 5.6E-99 score: 334.0
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 168..406

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0018327.1	Tan0018327.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009695	jasmonic acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0008970	phospholipase A1 activity