Tan0018230 (gene) Snake gourd v1

BLAST of Tan0018230 vs. ExPASy Swiss-Prot
Match: Q944G5 (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 646.7 bits (1667), Expect = 2.4e-184
Identity = 329/598 (55.02%), Postives = 424/598 (70.90%), Query Frame = 0

Query: 4   NEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNITAT 63
           N D V +   ++ K+ YRG K MPF++GNETFEKLG  G  SNLLVYLT VF++K+ TA 
Sbjct: 46  NTDVVDSFEEEQRKIVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAA 105

Query: 64  TIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLG-----------------MG 123
           TI+N F G+ NF T   AFLCDTYFGRYKTL  A IA FLG                  G
Sbjct: 106 TIINAFSGTINFGTFIAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACG 165

Query: 124 TGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWY 183
                  P+  Q+ FLL GLG +++GAGGIRPCNLAFGADQFNP + SGK GINSFFNWY
Sbjct: 166 NKISCEGPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWY 225

Query: 184 YFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEPHGSPFT 243
           +FTFTFA +ISLT +VY+Q+ VSW  GL IP  LMFL+C +FF G  +YVK++  GSP  
Sbjct: 226 FFTFTFAQIISLTAVVYIQSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLA 285

Query: 244 SVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIITEEDKLN 303
            + RV+ AA KKR L      W +L+N++PSN  N+ L YT QF FLDKAAI+T E+KLN
Sbjct: 286 GIARVIAAAIKKRGLKPVKQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAAIMTPEEKLN 345

Query: 304 SDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQSDRRLII 363
           SDG+A+DPW LC+ QQVEEVKC+VRVIPIW A+ IY++A T Q TY VFQALQSDRRL  
Sbjct: 346 SDGTASDPWKLCTLQQVEEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGS 405

Query: 364 FGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGITLLQKM 423
            G           F+IPAA+Y +F M G+T++I FYDR+LVPSLRR+T  E GI+LLQ++
Sbjct: 406 GG-----------FRIPAATYVVFLMTGMTVFIIFYDRVLVPSLRRVTGLETGISLLQRI 465

Query: 424 GFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQLTLIGLS 483
           G G   AI+++ VS F+E++RRN ALT+P L    A R G ISSMSALWL+PQLTL G++
Sbjct: 466 GAGFTFAIMSLLVSGFIEERRRNFALTKPTL--GMAPRTGEISSMSALWLIPQLTLAGIA 525

Query: 484 EAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTA----GHWLP 543
           EAF  I Q+EFYYK+FPENM+S  GS+ ++G  +S+YL+ F+++ VH+ TA    G+WL 
Sbjct: 526 EAFAAIGQMEFYYKQFPENMKSFAGSIFYVGAGVSSYLASFLISTVHRTTAHSPSGNWLA 585

Query: 544 EDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTVL 581
           EDLN+ +LDYFYF+++ L VVN+ YF++ ++WY+YKG     + E++  + E ++  L
Sbjct: 586 EDLNKAKLDYFYFMLTGLMVVNMAYFLLMARWYRYKGGNDEDITEIETNEEETKQQQL 630

BLAST of Tan0018230 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 7.6e-178
Identity = 321/578 (55.54%), Postives = 406/578 (70.24%), Query Frame = 0

Query: 6   DNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNITATTI 65
           D+V  +  ++ KV YRG K MPF++GNETFEKLG  G  SNLLVYLT VF++K+ITA TI
Sbjct: 30  DSVEEDVQNQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATI 89

Query: 66  MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLG-----------------MGTG 125
           +N F G+ NF T   AFLCDTYFGRYKTL  A IA FLG                  GT 
Sbjct: 90  INAFSGTINFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTA 149

Query: 126 GCLV--EPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWY 185
              +   P+  Q+AFLL GLG +++GAGGIRPCNLAFGADQFNP + SGK GI+SFFNWY
Sbjct: 150 ADSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWY 209

Query: 186 YFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEPHGSPFT 245
           +FTFTFA ++SLT++VYVQ+ VSW  GL IPA LMFL+C +FF G  +YVK++  GSP  
Sbjct: 210 FFTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLA 269

Query: 246 SVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIITEEDKLN 305
            + +V+  A KKR L      W +L+NY P    NS L YT QF FLDKAAI+T EDKL 
Sbjct: 270 GIAQVIAVAIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAAILTPEDKLQ 329

Query: 306 SDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQSDRRLII 365
            DG  ADPW LC+ QQVEEVKC+VRV+PIW A+ IY++  TQQ TY VFQALQSDRRL  
Sbjct: 330 PDGKPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGS 389

Query: 366 FGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGITLLQKM 425
            G           F IPAA+Y +F M G+T++I  YDR+LVP++RRIT  + GITLLQ++
Sbjct: 390 GG-----------FVIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRI 449

Query: 426 GFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQLTLIGLS 485
           G G+  A  ++ V+ FVE++RR  ALT+P L    A R+G ISSMSA+WL+PQL+L G++
Sbjct: 450 GTGIFFATASLVVAGFVEERRRTFALTKPTL--GMAPRKGEISSMSAMWLIPQLSLAGVA 509

Query: 486 EAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLT----AGHWLP 545
           EAF  I Q+EFYYK+FPENMRS  GS+ ++G  +S+YL  F++  VH+ T     G+WL 
Sbjct: 510 EAFAAIGQMEFYYKQFPENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLA 569

Query: 546 EDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGS 561
           EDLN+GRLD FYF+++ +  VN  YF+V S+WY+YKGS
Sbjct: 570 EDLNKGRLDLFYFMIAGILAVNFAYFLVMSRWYRYKGS 594

BLAST of Tan0018230 vs. ExPASy Swiss-Prot
Match: Q9M9V7 (Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 1.2e-175
Identity = 323/601 (53.74%), Postives = 424/601 (70.55%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           +E+ E N+  +  DE K+ YRG K MPF++GNETFEKLG  G SSNL++YLT VF+MK+I
Sbjct: 3   VEKTEKNIAED--DESKIIYRGWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSI 62

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLG------------MGTG 120
           TA  ++NI+ G++NF T+  AFLCD+YFGRYKTL +A IA FLG            +   
Sbjct: 63  TAAKVVNIYGGTSNFGTIVAAFLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPA 122

Query: 121 GCLVE-------PTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
            C  E       P+  Q+ FL   + L++IGAGGIRPCNL FGADQF+P T  GK GI S
Sbjct: 123 QCAKEIGSVCNGPSIGQIMFLAGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIES 182

Query: 181 FFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEPH 240
           FFNWY+FTFTFA M+SLT+IVYVQ+ VSW  GLAIPA LM L C +FF GS +YVK++  
Sbjct: 183 FFNWYFFTFTFAQMVSLTLIVYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKAS 242

Query: 241 GSPFTSVMRVLVAAFKKRRL-PLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIIT 300
           GSP  S+ RV+V A KKRRL P+  ++   L+NY+ S+  NS L +T QF FLDK+AI T
Sbjct: 243 GSPIHSITRVIVVAIKKRRLKPVGPNE---LYNYIASDFKNSKLGHTEQFRFLDKSAIQT 302

Query: 301 EEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQS 360
           ++DKLN DGS  D W LCS QQVEEVKC++RV+P+W +A ++++A  QQ TY +FQ+LQS
Sbjct: 303 QDDKLNKDGSPVDAWKLCSMQQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQS 362

Query: 361 DRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGI 420
           DRRL              +F+IPA SYT+F M+G+TI+IP YDR+LVP LR+ T ++GGI
Sbjct: 363 DRRL-----------GPGSFQIPAGSYTVFLMLGMTIFIPIYDRVLVPFLRKYTGRDGGI 422

Query: 421 TLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQL 480
           T LQ++G G+ + I +M VSA VEQ RR +ALT+P L    A R+GAISSMS +WL+PQL
Sbjct: 423 TQLQRVGAGLFLCITSMMVSAIVEQYRRKVALTKPTL--GLAPRKGAISSMSGMWLIPQL 482

Query: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLT---- 540
            L+G+++A   + Q+EFYYK+FPENMRS  GSL + G+ L++YLS F+++ VH  T    
Sbjct: 483 VLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYYCGIGLASYLSTFLLSAVHDTTEGFS 542

Query: 541 AGHWLPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFE 578
            G WLPEDLN+GRL+YFYFLV+ +  +NL YF++ S WY+YK   +   D MD    EF+
Sbjct: 543 GGSWLPEDLNKGRLEYFYFLVAGMMTLNLAYFLLVSHWYRYKDVVAKDKD-MDKTSAEFD 584

BLAST of Tan0018230 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.1e-128
Identity = 254/573 (44.33%), Postives = 363/573 (63.35%), Query Frame = 0

Query: 13  LDEPKVNYR--GVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNITATTIMNIFH 72
           LD  KV  +  G +A+ F++GNET E+LG+ G+ +N +VYLT VFH++ + A  ++NI+ 
Sbjct: 43  LDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWS 102

Query: 73  GSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGMGT------------GGCLVE----- 132
           G TN + L GA++ DTY GR+KT+ +AS A+ LG+ T              C  +     
Sbjct: 103 GFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSC 162

Query: 133 --PTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFTFT 192
             P   Q+  LL GL  + +G+GGIRPC++ FG DQF+  T  G  G+ SFFNWYY TFT
Sbjct: 163 GGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFT 222

Query: 193 FAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEPHGSPFTSVMRV 252
             ++I+ T++VY+Q +VSW  G +IP  LM L+  +FF G   YV ++P GS F+ + +V
Sbjct: 223 VVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQV 282

Query: 253 LVAAFKKRRLPLPDHQWPSLFNYMPS--NSINSNLPYTHQFSFLDKAAIITEEDKLNSDG 312
           +VAA KKR+L LP     ++  Y P+  +S+ S L  ++QF  LDKAA++ E D L  +G
Sbjct: 283 IVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEGD-LTPEG 342

Query: 313 SAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQSDRRLIIFGN 372
             AD W LCS Q+VEEVKCL+R++PIW+A II   A T Q T+ V QAL+ DR L     
Sbjct: 343 PPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNL----- 402

Query: 373 TTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGITLLQKMGFG 432
                     F+IPA S ++ +++ + I++PFYDR+ VP +RRIT  + GITLLQ++G G
Sbjct: 403 -------GPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTG 462

Query: 433 MLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQLTLIGLSEAF 492
           ++ AI +M V+  VE+ RR  ++         AG    ++ MS  WL PQL L+GL EAF
Sbjct: 463 IVFAIFSMIVAGIVERMRRIRSIN--------AGDPTGMTPMSVFWLSPQLILMGLCEAF 522

Query: 493 TVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGH----WLPEDL 552
            +I Q+EF+  +FPE+MRSI  SL  L  A S+YLS F+VTVVH+ + GH    WL ++L
Sbjct: 523 NIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNL 582

Query: 553 NQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYK 559
           N G+LDYFY+L++ L VVNL YF   ++ Y+YK
Sbjct: 583 NAGKLDYFYYLIAVLGVVNLVYFWYCARGYRYK 594

BLAST of Tan0018230 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 432.2 bits (1110), Expect = 9.4e-120
Identity = 248/568 (43.66%), Postives = 345/568 (60.74%), Query Frame = 0

Query: 16  PKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNITATTIMNIFHGSTNF 75
           P+    G +A+ F++GNET EKLG+ G+S+N ++YL +VFHM+ + A  +  ++ G TNF
Sbjct: 11  PEKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNF 70

Query: 76  STLFGAFLCDTYFGRYKTLGYASIASFLGMGT---GGCLV----------------EPTP 135
           + L GA + D Y GR+KT+ YAS+ S LG+ T     CL                 +P  
Sbjct: 71  APLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNK 130

Query: 136 WQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFTFTFAMMI 195
            QL  L  GLG + IG+GGIRPC++ FG DQF+  T  G  G+ SFFNWYY T T  ++ 
Sbjct: 131 LQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIF 190

Query: 196 SLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEPHGSPFTSVMRVLVAAF 255
           S T++VY+QT VSW  G +IP  LM  +  LFF+G   YV ++P GS F+ + RV+VAA 
Sbjct: 191 SHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAAR 250

Query: 256 KKR--RLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIITEEDKLNSDGSAADP 315
           KKR  ++ L D      +       + S LP T QF FLDKAA+I + D L S+G  A+ 
Sbjct: 251 KKRDLKISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDGD-LTSEGVPANK 310

Query: 316 WTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQSDRRLIIFGNTTSST 375
           W LCS Q+VEEVKCL+RV+P+W+A II  VA T Q T++VFQA + DR +          
Sbjct: 311 WRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHM---------- 370

Query: 376 TTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGITLLQKMGFGMLIAI 435
               +F+IPAAS T+ + I + IW+P Y+ +LVP L R+  ++  +TLLQ+MG G++ AI
Sbjct: 371 --GPHFEIPAASITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAI 430

Query: 436 LTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQLTLIGLSEAFTVIAQ 495
           L+MF + FVE  RR               R   ++ MS  WL   L L+GL E+F  I  
Sbjct: 431 LSMFTAGFVEGVRRT--------------RATEMTQMSVFWLALPLILMGLCESFNFIGL 490

Query: 496 VEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQL--TAGH--WLPEDLNQGRL 555
           +EF+  +FPE+MRSI  SL  L  A +NYLS  +VT VH++  T  H  WL +DL++G+L
Sbjct: 491 IEFFNSQFPEHMRSIANSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKL 548

Query: 556 DYFYFLVSALEVVNLGYFVVFSKWYKYK 559
           DYFY+L++ L VVNL YF   +  Y+YK
Sbjct: 551 DYFYYLIAVLGVVNLVYFWYCAHRYQYK 548

BLAST of Tan0018230 vs. NCBI nr
Match: XP_038878529.1 (protein NRT1/ PTR FAMILY 2.10-like [Benincasa hispida])

HSP 1 Score: 1010.0 bits (2610), Expect = 8.4e-291
Identity = 516/601 (85.86%), Postives = 542/601 (90.18%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           MERN ++VH  NLDEPKVNY+GVKAMPFVVGNETFEKLGTTG SSNLLVYLTDVFHMKNI
Sbjct: 1   MERNNEDVH--NLDEPKVNYKGVKAMPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKNI 60

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGM--------------- 120
           TATT+MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIAS LGM               
Sbjct: 61  TATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLLGMVVLTLTATIEKLHPP 120

Query: 121 -----GTGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGIN 180
                  GG  +EPTPWQ+AFLLFGLGL++IGAGGIRPCNLAFGADQFNPNTASGKLGIN
Sbjct: 121 DCGKGSAGGPCLEPTPWQIAFLLFGLGLLVIGAGGIRPCNLAFGADQFNPNTASGKLGIN 180

Query: 181 SFFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEP 240
           SFFNWYYFTFTFAMMISLTIIVYVQTEVSW WGLAIPAFLMFLSCALFFMGSWIYVKLEP
Sbjct: 181 SFFNWYYFTFTFAMMISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP 240

Query: 241 HGSPFTSVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIIT 300
            GSPFTSVMRVL+AAFKKR+LPLPD QWPSLFN++P NSINS LP T QFSFLDKAAIIT
Sbjct: 241 DGSPFTSVMRVLMAAFKKRQLPLPDQQWPSLFNHIPPNSINSKLPCTDQFSFLDKAAIIT 300

Query: 301 EEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQS 360
            EDK  SDGSAADPWTLCSTQQVEEVKCLVRVIPIW AAIIYHVATTQQQTYVVFQALQS
Sbjct: 301 PEDKFKSDGSAADPWTLCSTQQVEEVKCLVRVIPIWGAAIIYHVATTQQQTYVVFQALQS 360

Query: 361 DRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGI 420
           DRRL  FGNT       H FKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITA+EGGI
Sbjct: 361 DRRL-FFGNT-------HYFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAREGGI 420

Query: 421 TLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQL 480
           TLLQKMG GM IAI+TMF+SA VEQKRRNLALTQP LC+E  GRRG++SSMSALWLVPQL
Sbjct: 421 TLLQKMGLGMGIAIVTMFISALVEQKRRNLALTQP-LCEE-TGRRGSVSSMSALWLVPQL 480

Query: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGHW 540
           TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSF+GLALSNYLSGFMVTVVH+++AG+W
Sbjct: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHRVSAGNW 540

Query: 541 LPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTVL 582
           LPEDLN+GRLDYFYFLVS LE VNLGYFV+ SKWYKYKGSGSHGVDEMDFGKTEFEKTV+
Sbjct: 541 LPEDLNEGRLDYFYFLVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVV 589

BLAST of Tan0018230 vs. NCBI nr
Match: XP_004138297.1 (protein NRT1/ PTR FAMILY 2.10 [Cucumis sativus] >KGN63648.1 hypothetical protein Csa_014382 [Cucumis sativus])

HSP 1 Score: 1010.0 bits (2610), Expect = 8.4e-291
Identity = 516/601 (85.86%), Postives = 540/601 (89.85%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           MERN ++V  NNLDEP VNYRGVKAMPFVVGNETFEKLGTTG SSNLLVYLTDVFHMKNI
Sbjct: 1   MERNNEDV--NNLDEPNVNYRGVKAMPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKNI 60

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGM--------------- 120
           TATT+MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIAS +GM               
Sbjct: 61  TATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPP 120

Query: 121 -----GTGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGIN 180
                  GG  +EPT WQ+AFLLFGLGL++IGAGGIRPCNLAFGADQFNPNTASGKLGIN
Sbjct: 121 DCGKGSAGGACLEPTSWQIAFLLFGLGLLVIGAGGIRPCNLAFGADQFNPNTASGKLGIN 180

Query: 181 SFFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEP 240
           SFFNWYYFTFTFAMMISLTIIVYVQTEVSW WGLAIPAFLMFLSCALFFMGSWIYVKLEP
Sbjct: 181 SFFNWYYFTFTFAMMISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP 240

Query: 241 HGSPFTSVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIIT 300
            GSPFTSVMRVL+AA KKR+LPLPD QWPSLFN++PSNSINS LPYT+QFSF DKAAIIT
Sbjct: 241 DGSPFTSVMRVLMAALKKRQLPLPDQQWPSLFNHIPSNSINSKLPYTNQFSFFDKAAIIT 300

Query: 301 EEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQS 360
            EDK  SDGSAADPWTLCSTQQVEEVKCLVRVIPIW AAIIYHVATTQQQTY VFQALQS
Sbjct: 301 SEDKFKSDGSAADPWTLCSTQQVEEVKCLVRVIPIWGAAIIYHVATTQQQTYAVFQALQS 360

Query: 361 DRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGI 420
           DRRL  FGNT       H FKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITA+EGGI
Sbjct: 361 DRRL-FFGNT-------HYFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAREGGI 420

Query: 421 TLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQL 480
           TLLQKMGFGM IAI+TMF+SA VEQKRRNLALTQP LC+E  GRRG+ISSMSALWLVPQL
Sbjct: 421 TLLQKMGFGMGIAIITMFISALVEQKRRNLALTQP-LCEE-TGRRGSISSMSALWLVPQL 480

Query: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGHW 540
           TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSF+GLALSNYLSGFMVTVVH+LTAG W
Sbjct: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHRLTAGKW 540

Query: 541 LPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTVL 582
           LPEDLN+GRLDYFYFLVS LE VNLGYF++ SKWYKYKGSGSHGVDEMDFGKTEFEKTV+
Sbjct: 541 LPEDLNKGRLDYFYFLVSGLEAVNLGYFLLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVV 589

BLAST of Tan0018230 vs. NCBI nr
Match: XP_022921698.1 (protein NRT1/ PTR FAMILY 2.10-like [Cucurbita moschata])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.8e-289
Identity = 512/602 (85.05%), Postives = 538/602 (89.37%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           MERN ++VH  NLDE KVNY+GVKA+PFVVGNETFEKLGTTG SSNLLVYLTDVFHMK I
Sbjct: 1   MERNNEDVH--NLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTI 60

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGM--------------- 120
           TATT+MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIAS +GM               
Sbjct: 61  TATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPP 120

Query: 121 ------GTGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGI 180
                  TGG  +EPTPWQ+AFLLFGLGL+IIGAGGIRPCNLAFGADQFNPNTASGKLGI
Sbjct: 121 DCGKGSATGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGI 180

Query: 181 NSFFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLE 240
           NSFFNWYYFTFTFAM+ISLTIIVYVQTEVSW WGLAIPAFLMFLSCALFFMGSWIYVKLE
Sbjct: 181 NSFFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLE 240

Query: 241 PHGSPFTSVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAII 300
           P GSPFTSVMRVLVAA KKRRLP PD QWPSLFN++PSNSINS LPYT QFSFL+KAAII
Sbjct: 241 PEGSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAII 300

Query: 301 TEEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQ 360
           T EDK  SDGSA DPWTLCSTQQVEEVKCLVRVIPIW AAI+YHVATTQQQTYVVFQALQ
Sbjct: 301 TPEDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQ 360

Query: 361 SDRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGG 420
           SDRRL  FGNT       H FKIPAASYTIFTMIGLTIWIPFYDRI+VPSLRRITA+EGG
Sbjct: 361 SDRRL-FFGNT-------HYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITAREGG 420

Query: 421 ITLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQ 480
           ITLLQKMG GM+IAI+TMF+SA VEQKRRNLALTQP LC+E  GRRGAISSMSALWLVPQ
Sbjct: 421 ITLLQKMGIGMVIAIVTMFISALVEQKRRNLALTQP-LCEE-TGRRGAISSMSALWLVPQ 480

Query: 481 LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGH 540
           LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGS SF+GLALSNYLSGFMVTVVH LT G+
Sbjct: 481 LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSFSFVGLALSNYLSGFMVTVVHSLTTGN 540

Query: 541 WLPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTV 582
           WLPEDLN+GRLDYFYF+VS LE VNLGYFV+ SKWYKYKGSGSHGVDEMDFGKTEFEKTV
Sbjct: 541 WLPEDLNEGRLDYFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTV 590

BLAST of Tan0018230 vs. NCBI nr
Match: XP_023515678.1 (protein NRT1/ PTR FAMILY 2.10-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.9e-288
Identity = 510/602 (84.72%), Postives = 537/602 (89.20%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           MERN +++H  NLDE KVNY+GVKA+PFVVGNETFEKLGTTG SSNLLVYLTDVFHMK I
Sbjct: 1   MERNNEDIH--NLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTI 60

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGM--------------- 120
           TATT+MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIAS +GM               
Sbjct: 61  TATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPP 120

Query: 121 ------GTGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGI 180
                   GG  +EPTPWQ+AFLLFGLGL+IIGAGGIRPCNLAFGADQFNPNTASGKLGI
Sbjct: 121 DCGKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGI 180

Query: 181 NSFFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLE 240
           NSFFNWYYFTFTFAM+ISLTIIVYVQTEVSW WGLAIPAFLMFLSCALFFMGSWIYVKLE
Sbjct: 181 NSFFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLE 240

Query: 241 PHGSPFTSVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAII 300
           P GSPFTSVMRVLVAA KKRRLP PD QWPSLFN++PSNSINS LPYT QFSFL+KAAII
Sbjct: 241 PEGSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAII 300

Query: 301 TEEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQ 360
           T EDK  SDGSA DPWTLCSTQQVEEVKCLVRVIPIW AAI+YHVATTQQQTYVVFQALQ
Sbjct: 301 TPEDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQ 360

Query: 361 SDRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGG 420
           SDRRL  FGNT       H FKIPAASYTIFTMIGLTIWIPFYDRI+VPSLRRITA+EGG
Sbjct: 361 SDRRL-FFGNT-------HYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITAREGG 420

Query: 421 ITLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQ 480
           ITLLQKMG GM+IAI+TMF+SA VEQKRRNLALTQP LC+E  GRRGAISSMSALWLVPQ
Sbjct: 421 ITLLQKMGIGMVIAIVTMFISALVEQKRRNLALTQP-LCEE-TGRRGAISSMSALWLVPQ 480

Query: 481 LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGH 540
           LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGS SF+GLALSNYLSGFMVTVVH LT G+
Sbjct: 481 LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSFSFVGLALSNYLSGFMVTVVHSLTTGN 540

Query: 541 WLPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTV 582
           WLPEDLN+GRLDYFYF+VS LE VNLGYFV+ SKWYKYKGSGSHGVDEMDFGKTEFEKTV
Sbjct: 541 WLPEDLNEGRLDYFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTV 590

BLAST of Tan0018230 vs. NCBI nr
Match: KAG7023179.1 (Protein NRT1/ PTR FAMILY 2.11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.9e-288
Identity = 510/602 (84.72%), Postives = 535/602 (88.87%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           MERN ++VH  NLDE KVNY+GVKA+PFVVGNETFEKLGTTG SSNLLVYLTDVFHMK I
Sbjct: 1   MERNNEDVH--NLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTI 60

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGM--------------- 120
           TATT+MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIAS +GM               
Sbjct: 61  TATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPP 120

Query: 121 ------GTGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGI 180
                   GG  +EPTPWQ+AFLLFGLGL+IIGAGGIRPCNLAFGADQFNPNTASGKLGI
Sbjct: 121 DCGKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGI 180

Query: 181 NSFFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLE 240
           NSFFNWYYFTFTFAM+ISLTIIVYVQTEVSW WGLAIPAFLMFLSCALFFMGSWIYVKLE
Sbjct: 181 NSFFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLE 240

Query: 241 PHGSPFTSVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAII 300
           P GSPFTSVMRVLVAA KKRRLP PD QWPSLFN++PSNSINS LPYT QFSFL+KAAII
Sbjct: 241 PEGSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAII 300

Query: 301 TEEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQ 360
           T EDK  SDGSA DPWTLCSTQQVEEVKCLVRVIPIW AAI+YHVATTQQQTYVVFQALQ
Sbjct: 301 TPEDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQ 360

Query: 361 SDRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGG 420
           SDRRL  FGNT       H FKIPAASYTIFTMIGLTIWIPFYDRI+VPSLRRITA+EGG
Sbjct: 361 SDRRL-FFGNT-------HYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITAREGG 420

Query: 421 ITLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQ 480
           ITLLQKMG GM+IAI+TMF+SA VEQKRRNLALTQPL   E  GRRGAISSMSALWLVPQ
Sbjct: 421 ITLLQKMGIGMVIAIVTMFISALVEQKRRNLALTQPL--SEETGRRGAISSMSALWLVPQ 480

Query: 481 LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGH 540
           LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGS SF+GLALSNYLSGFMVTVVH LT G+
Sbjct: 481 LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSFSFVGLALSNYLSGFMVTVVHSLTTGN 540

Query: 541 WLPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTV 582
           WLPEDLN+GRLDYFYF+VS LE VNLGYFV+ SKWYKYKGSGSHGVDEMDFGKTEFEKTV
Sbjct: 541 WLPEDLNEGRLDYFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTV 590

BLAST of Tan0018230 vs. ExPASy TrEMBL
Match: A0A0A0LPB6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G008540 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 4.0e-291
Identity = 516/601 (85.86%), Postives = 540/601 (89.85%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           MERN ++V  NNLDEP VNYRGVKAMPFVVGNETFEKLGTTG SSNLLVYLTDVFHMKNI
Sbjct: 1   MERNNEDV--NNLDEPNVNYRGVKAMPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKNI 60

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGM--------------- 120
           TATT+MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIAS +GM               
Sbjct: 61  TATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPP 120

Query: 121 -----GTGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGIN 180
                  GG  +EPT WQ+AFLLFGLGL++IGAGGIRPCNLAFGADQFNPNTASGKLGIN
Sbjct: 121 DCGKGSAGGACLEPTSWQIAFLLFGLGLLVIGAGGIRPCNLAFGADQFNPNTASGKLGIN 180

Query: 181 SFFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEP 240
           SFFNWYYFTFTFAMMISLTIIVYVQTEVSW WGLAIPAFLMFLSCALFFMGSWIYVKLEP
Sbjct: 181 SFFNWYYFTFTFAMMISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP 240

Query: 241 HGSPFTSVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIIT 300
            GSPFTSVMRVL+AA KKR+LPLPD QWPSLFN++PSNSINS LPYT+QFSF DKAAIIT
Sbjct: 241 DGSPFTSVMRVLMAALKKRQLPLPDQQWPSLFNHIPSNSINSKLPYTNQFSFFDKAAIIT 300

Query: 301 EEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQS 360
            EDK  SDGSAADPWTLCSTQQVEEVKCLVRVIPIW AAIIYHVATTQQQTY VFQALQS
Sbjct: 301 SEDKFKSDGSAADPWTLCSTQQVEEVKCLVRVIPIWGAAIIYHVATTQQQTYAVFQALQS 360

Query: 361 DRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGI 420
           DRRL  FGNT       H FKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITA+EGGI
Sbjct: 361 DRRL-FFGNT-------HYFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAREGGI 420

Query: 421 TLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQL 480
           TLLQKMGFGM IAI+TMF+SA VEQKRRNLALTQP LC+E  GRRG+ISSMSALWLVPQL
Sbjct: 421 TLLQKMGFGMGIAIITMFISALVEQKRRNLALTQP-LCEE-TGRRGSISSMSALWLVPQL 480

Query: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGHW 540
           TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSF+GLALSNYLSGFMVTVVH+LTAG W
Sbjct: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHRLTAGKW 540

Query: 541 LPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTVL 582
           LPEDLN+GRLDYFYFLVS LE VNLGYF++ SKWYKYKGSGSHGVDEMDFGKTEFEKTV+
Sbjct: 541 LPEDLNKGRLDYFYFLVSGLEAVNLGYFLLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVV 589

BLAST of Tan0018230 vs. ExPASy TrEMBL
Match: A0A6J1E4K9 (protein NRT1/ PTR FAMILY 2.10-like OS=Cucurbita moschata OX=3662 GN=LOC111429868 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.8e-289
Identity = 512/602 (85.05%), Postives = 538/602 (89.37%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           MERN ++VH  NLDE KVNY+GVKA+PFVVGNETFEKLGTTG SSNLLVYLTDVFHMK I
Sbjct: 1   MERNNEDVH--NLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTI 60

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGM--------------- 120
           TATT+MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIAS +GM               
Sbjct: 61  TATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPP 120

Query: 121 ------GTGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGI 180
                  TGG  +EPTPWQ+AFLLFGLGL+IIGAGGIRPCNLAFGADQFNPNTASGKLGI
Sbjct: 121 DCGKGSATGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGI 180

Query: 181 NSFFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLE 240
           NSFFNWYYFTFTFAM+ISLTIIVYVQTEVSW WGLAIPAFLMFLSCALFFMGSWIYVKLE
Sbjct: 181 NSFFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLE 240

Query: 241 PHGSPFTSVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAII 300
           P GSPFTSVMRVLVAA KKRRLP PD QWPSLFN++PSNSINS LPYT QFSFL+KAAII
Sbjct: 241 PEGSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAII 300

Query: 301 TEEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQ 360
           T EDK  SDGSA DPWTLCSTQQVEEVKCLVRVIPIW AAI+YHVATTQQQTYVVFQALQ
Sbjct: 301 TPEDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQ 360

Query: 361 SDRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGG 420
           SDRRL  FGNT       H FKIPAASYTIFTMIGLTIWIPFYDRI+VPSLRRITA+EGG
Sbjct: 361 SDRRL-FFGNT-------HYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITAREGG 420

Query: 421 ITLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQ 480
           ITLLQKMG GM+IAI+TMF+SA VEQKRRNLALTQP LC+E  GRRGAISSMSALWLVPQ
Sbjct: 421 ITLLQKMGIGMVIAIVTMFISALVEQKRRNLALTQP-LCEE-TGRRGAISSMSALWLVPQ 480

Query: 481 LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGH 540
           LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGS SF+GLALSNYLSGFMVTVVH LT G+
Sbjct: 481 LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSFSFVGLALSNYLSGFMVTVVHSLTTGN 540

Query: 541 WLPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTV 582
           WLPEDLN+GRLDYFYF+VS LE VNLGYFV+ SKWYKYKGSGSHGVDEMDFGKTEFEKTV
Sbjct: 541 WLPEDLNEGRLDYFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTV 590

BLAST of Tan0018230 vs. ExPASy TrEMBL
Match: A0A5A7UPX7 (Protein NRT1/ PTR FAMILY 2.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002260 PE=3 SV=1)

HSP 1 Score: 1000.7 bits (2586), Expect = 2.5e-288
Identity = 509/601 (84.69%), Postives = 536/601 (89.18%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           MERN ++VH  NLDEPKVNY+GVKA+PFVVGNETFEKLGTTG SSNLLVYLTDVFHMKNI
Sbjct: 1   MERNNEDVH--NLDEPKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKNI 60

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGM--------------- 120
           TATT+MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIAS +GM               
Sbjct: 61  TATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPP 120

Query: 121 -----GTGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGIN 180
                  GG  +EPTPWQ+AFLLFGLGL++IGAGGIRPCNLAFGADQFNPNT SGKLGIN
Sbjct: 121 DCGKGSAGGACLEPTPWQIAFLLFGLGLLVIGAGGIRPCNLAFGADQFNPNTVSGKLGIN 180

Query: 181 SFFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEP 240
           SFFNWYYFTF FAMMISLTIIVYVQTEVSW WGLAIPAFLMFLSCALFFMGSWIYVKLEP
Sbjct: 181 SFFNWYYFTFKFAMMISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP 240

Query: 241 HGSPFTSVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIIT 300
            GSPFTSV+RVL+AA KKR+LPLPD QW SLFN++PSNSINS LPYT+Q SFLDKAAIIT
Sbjct: 241 DGSPFTSVLRVLMAALKKRQLPLPDQQWSSLFNHIPSNSINSKLPYTNQLSFLDKAAIIT 300

Query: 301 EEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQS 360
            +DK  SDGSAADPWTLCSTQQVEEVKCLVRVIPIW AAIIYHVATTQQQTY VFQALQS
Sbjct: 301 SDDKFKSDGSAADPWTLCSTQQVEEVKCLVRVIPIWCAAIIYHVATTQQQTYAVFQALQS 360

Query: 361 DRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGI 420
           DRRL  FGNT       H FKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITA+EGGI
Sbjct: 361 DRRL-FFGNT-------HYFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAREGGI 420

Query: 421 TLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQL 480
           TLLQKMG G+ IAI+TMF+SA VEQKRRNLALTQPL   E  GRRG+ISSMSALWLVPQL
Sbjct: 421 TLLQKMGLGLGIAIITMFISALVEQKRRNLALTQPL--YEERGRRGSISSMSALWLVPQL 480

Query: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGHW 540
           TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSF+GLALSNYLSGFMVTVVH+LTAG W
Sbjct: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHRLTAGKW 540

Query: 541 LPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTVL 582
           LPEDLN+GRLDYFYFLVS LE VNLGYFV+ SKWYKYKGSGSHGVDEMDFGKTEFEKTV+
Sbjct: 541 LPEDLNEGRLDYFYFLVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVV 589

BLAST of Tan0018230 vs. ExPASy TrEMBL
Match: A0A6J1JCU2 (protein NRT1/ PTR FAMILY 2.10-like OS=Cucurbita maxima OX=3661 GN=LOC111485624 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.5e-288
Identity = 510/602 (84.72%), Postives = 537/602 (89.20%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           MERN ++VH  NLDE KVNY+GVKA+PFVVGNETFEKLGTTG SSNLLVYLTDVFHMK I
Sbjct: 1   MERNNEDVH--NLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTI 60

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGM--------------- 120
           TATT+MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIAS +GM               
Sbjct: 61  TATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPP 120

Query: 121 ------GTGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGI 180
                   GG  +EPTPWQ+AFLLFGLGL+IIGAGGIRPCNLAFGADQFNPNTASGKLGI
Sbjct: 121 DCGKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGI 180

Query: 181 NSFFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLE 240
           NSFFNWYYFTFTFAM+ISLTIIVYVQTEVSW WGLAIPAFLMFLSCALFFMGSWIYVKLE
Sbjct: 181 NSFFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLE 240

Query: 241 PHGSPFTSVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAII 300
           P GSPFTSVMRVLVAA KKRRLP PD QWPSLFN++PSNSINS LPYT QFSFL+KAAII
Sbjct: 241 PQGSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAII 300

Query: 301 TEEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQ 360
           T EDK  SDGSA DPWTLCSTQQVEEVKCLVRVIPIW AAI+YHVATTQQQTYVVFQALQ
Sbjct: 301 TPEDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQ 360

Query: 361 SDRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGG 420
           SDRRL  FGNT       H FKIPAASYTIFTMIGLTIWIPFYDRI+VPSLRRIT++EGG
Sbjct: 361 SDRRL-FFGNT-------HYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGG 420

Query: 421 ITLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQ 480
           ITLLQKMG GM+IAI+TMF+SA VEQKRRNLALTQP LC+E  GRR AISSMSALWLVPQ
Sbjct: 421 ITLLQKMGIGMVIAIVTMFISALVEQKRRNLALTQP-LCEE-TGRRAAISSMSALWLVPQ 480

Query: 481 LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGH 540
           LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSF+GLALSNYLSGFMVTVVH LT G+
Sbjct: 481 LTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGN 540

Query: 541 WLPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTV 582
           WLPEDLN+GRLDYFYF+VS LE VNLGYFV+ SKWYKYKGSGSHGVDEMDFGKTEFEKTV
Sbjct: 541 WLPEDLNEGRLDYFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTV 590

BLAST of Tan0018230 vs. ExPASy TrEMBL
Match: A0A5D3BIU4 (Protein NRT1/ PTR FAMILY 2.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002340 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.5e-288
Identity = 509/601 (84.69%), Postives = 536/601 (89.18%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           MERN ++V   NLDEPKVNY+GVKA+PFVVGNETFEKLGTTG SSNLLVYLTDVFHMKNI
Sbjct: 1   MERNNEDV--RNLDEPKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKNI 60

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGM--------------- 120
           TATT+MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIAS +GM               
Sbjct: 61  TATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPP 120

Query: 121 -----GTGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGIN 180
                  GG  +EPTPWQ+AFLLFGLGL++IGAGGIRPCNLAFGADQFNPNT SGKLGIN
Sbjct: 121 DCGKGSAGGACLEPTPWQIAFLLFGLGLLVIGAGGIRPCNLAFGADQFNPNTVSGKLGIN 180

Query: 181 SFFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEP 240
           SFFNWYYFTFTFAMMISLTIIVYVQTEVSW WGLAIPAFLMFLSCALFFMGSWIYVKLEP
Sbjct: 181 SFFNWYYFTFTFAMMISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP 240

Query: 241 HGSPFTSVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIIT 300
            GSPFTSV+RVL+AA KKR+LPLPD QW SLFN++PSNSINS LPYT+Q SFLDKAAIIT
Sbjct: 241 DGSPFTSVLRVLMAALKKRQLPLPDQQWSSLFNHIPSNSINSKLPYTNQLSFLDKAAIIT 300

Query: 301 EEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQS 360
            +DK  SDGSAADPWTLCSTQQVEEVKCLVRVIPIW AAIIYHVATTQQQTY VFQALQS
Sbjct: 301 SDDKFKSDGSAADPWTLCSTQQVEEVKCLVRVIPIWCAAIIYHVATTQQQTYAVFQALQS 360

Query: 361 DRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGI 420
           DRRL  FGNT       H FKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITA+EGGI
Sbjct: 361 DRRL-FFGNT-------HYFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAREGGI 420

Query: 421 TLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQL 480
           TLLQKMG G+ IAI+TMF+SA VEQKRRNLALTQPL   E  GRRG+ISSMSALWLVPQL
Sbjct: 421 TLLQKMGLGLGIAIITMFISALVEQKRRNLALTQPL--YEERGRRGSISSMSALWLVPQL 480

Query: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGHW 540
           TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSF+GLALSNYLSGFMVTVVH+LTAG W
Sbjct: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHRLTAGKW 540

Query: 541 LPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTVL 582
           LPEDLN+GRLDYFYFLVS LE VNLGYFV+ SKWYKYKGSGSHGVDEMDFGKTEFEKTV+
Sbjct: 541 LPEDLNEGRLDYFYFLVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVV 589

BLAST of Tan0018230 vs. TAIR 10
Match: AT3G47960.1 (Major facilitator superfamily protein )

HSP 1 Score: 646.7 bits (1667), Expect = 1.7e-185
Identity = 329/598 (55.02%), Postives = 424/598 (70.90%), Query Frame = 0

Query: 4   NEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNITAT 63
           N D V +   ++ K+ YRG K MPF++GNETFEKLG  G  SNLLVYLT VF++K+ TA 
Sbjct: 46  NTDVVDSFEEEQRKIVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAA 105

Query: 64  TIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLG-----------------MG 123
           TI+N F G+ NF T   AFLCDTYFGRYKTL  A IA FLG                  G
Sbjct: 106 TIINAFSGTINFGTFIAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACG 165

Query: 124 TGGCLVEPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWY 183
                  P+  Q+ FLL GLG +++GAGGIRPCNLAFGADQFNP + SGK GINSFFNWY
Sbjct: 166 NKISCEGPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWY 225

Query: 184 YFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEPHGSPFT 243
           +FTFTFA +ISLT +VY+Q+ VSW  GL IP  LMFL+C +FF G  +YVK++  GSP  
Sbjct: 226 FFTFTFAQIISLTAVVYIQSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLA 285

Query: 244 SVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIITEEDKLN 303
            + RV+ AA KKR L      W +L+N++PSN  N+ L YT QF FLDKAAI+T E+KLN
Sbjct: 286 GIARVIAAAIKKRGLKPVKQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAAIMTPEEKLN 345

Query: 304 SDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQSDRRLII 363
           SDG+A+DPW LC+ QQVEEVKC+VRVIPIW A+ IY++A T Q TY VFQALQSDRRL  
Sbjct: 346 SDGTASDPWKLCTLQQVEEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGS 405

Query: 364 FGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGITLLQKM 423
            G           F+IPAA+Y +F M G+T++I FYDR+LVPSLRR+T  E GI+LLQ++
Sbjct: 406 GG-----------FRIPAATYVVFLMTGMTVFIIFYDRVLVPSLRRVTGLETGISLLQRI 465

Query: 424 GFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQLTLIGLS 483
           G G   AI+++ VS F+E++RRN ALT+P L    A R G ISSMSALWL+PQLTL G++
Sbjct: 466 GAGFTFAIMSLLVSGFIEERRRNFALTKPTL--GMAPRTGEISSMSALWLIPQLTLAGIA 525

Query: 484 EAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTA----GHWLP 543
           EAF  I Q+EFYYK+FPENM+S  GS+ ++G  +S+YL+ F+++ VH+ TA    G+WL 
Sbjct: 526 EAFAAIGQMEFYYKQFPENMKSFAGSIFYVGAGVSSYLASFLISTVHRTTAHSPSGNWLA 585

Query: 544 EDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFEKTVL 581
           EDLN+ +LDYFYF+++ L VVN+ YF++ ++WY+YKG     + E++  + E ++  L
Sbjct: 586 EDLNKAKLDYFYFMLTGLMVVNMAYFLLMARWYRYKGGNDEDITEIETNEEETKQQQL 630

BLAST of Tan0018230 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 625.2 bits (1611), Expect = 5.4e-179
Identity = 321/578 (55.54%), Postives = 406/578 (70.24%), Query Frame = 0

Query: 6   DNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNITATTI 65
           D+V  +  ++ KV YRG K MPF++GNETFEKLG  G  SNLLVYLT VF++K+ITA TI
Sbjct: 30  DSVEEDVQNQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATI 89

Query: 66  MNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLG-----------------MGTG 125
           +N F G+ NF T   AFLCDTYFGRYKTL  A IA FLG                  GT 
Sbjct: 90  INAFSGTINFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTA 149

Query: 126 GCLV--EPTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWY 185
              +   P+  Q+AFLL GLG +++GAGGIRPCNLAFGADQFNP + SGK GI+SFFNWY
Sbjct: 150 ADSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWY 209

Query: 186 YFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEPHGSPFT 245
           +FTFTFA ++SLT++VYVQ+ VSW  GL IPA LMFL+C +FF G  +YVK++  GSP  
Sbjct: 210 FFTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLA 269

Query: 246 SVMRVLVAAFKKRRLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIITEEDKLN 305
            + +V+  A KKR L      W +L+NY P    NS L YT QF FLDKAAI+T EDKL 
Sbjct: 270 GIAQVIAVAIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAAILTPEDKLQ 329

Query: 306 SDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQSDRRLII 365
            DG  ADPW LC+ QQVEEVKC+VRV+PIW A+ IY++  TQQ TY VFQALQSDRRL  
Sbjct: 330 PDGKPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGS 389

Query: 366 FGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGITLLQKM 425
            G           F IPAA+Y +F M G+T++I  YDR+LVP++RRIT  + GITLLQ++
Sbjct: 390 GG-----------FVIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRI 449

Query: 426 GFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQLTLIGLS 485
           G G+  A  ++ V+ FVE++RR  ALT+P L    A R+G ISSMSA+WL+PQL+L G++
Sbjct: 450 GTGIFFATASLVVAGFVEERRRTFALTKPTL--GMAPRKGEISSMSAMWLIPQLSLAGVA 509

Query: 486 EAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLT----AGHWLP 545
           EAF  I Q+EFYYK+FPENMRS  GS+ ++G  +S+YL  F++  VH+ T     G+WL 
Sbjct: 510 EAFAAIGQMEFYYKQFPENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLA 569

Query: 546 EDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGS 561
           EDLN+GRLD FYF+++ +  VN  YF+V S+WY+YKGS
Sbjct: 570 EDLNKGRLDLFYFMIAGILAVNFAYFLVMSRWYRYKGS 594

BLAST of Tan0018230 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 617.8 bits (1592), Expect = 8.6e-177
Identity = 323/601 (53.74%), Postives = 424/601 (70.55%), Query Frame = 0

Query: 1   MERNEDNVHNNNLDEPKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNI 60
           +E+ E N+  +  DE K+ YRG K MPF++GNETFEKLG  G SSNL++YLT VF+MK+I
Sbjct: 3   VEKTEKNIAED--DESKIIYRGWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSI 62

Query: 61  TATTIMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLG------------MGTG 120
           TA  ++NI+ G++NF T+  AFLCD+YFGRYKTL +A IA FLG            +   
Sbjct: 63  TAAKVVNIYGGTSNFGTIVAAFLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPA 122

Query: 121 GCLVE-------PTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
            C  E       P+  Q+ FL   + L++IGAGGIRPCNL FGADQF+P T  GK GI S
Sbjct: 123 QCAKEIGSVCNGPSIGQIMFLAGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIES 182

Query: 181 FFNWYYFTFTFAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEPH 240
           FFNWY+FTFTFA M+SLT+IVYVQ+ VSW  GLAIPA LM L C +FF GS +YVK++  
Sbjct: 183 FFNWYFFTFTFAQMVSLTLIVYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKAS 242

Query: 241 GSPFTSVMRVLVAAFKKRRL-PLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIIT 300
           GSP  S+ RV+V A KKRRL P+  ++   L+NY+ S+  NS L +T QF FLDK+AI T
Sbjct: 243 GSPIHSITRVIVVAIKKRRLKPVGPNE---LYNYIASDFKNSKLGHTEQFRFLDKSAIQT 302

Query: 301 EEDKLNSDGSAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQS 360
           ++DKLN DGS  D W LCS QQVEEVKC++RV+P+W +A ++++A  QQ TY +FQ+LQS
Sbjct: 303 QDDKLNKDGSPVDAWKLCSMQQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQS 362

Query: 361 DRRLIIFGNTTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGI 420
           DRRL              +F+IPA SYT+F M+G+TI+IP YDR+LVP LR+ T ++GGI
Sbjct: 363 DRRL-----------GPGSFQIPAGSYTVFLMLGMTIFIPIYDRVLVPFLRKYTGRDGGI 422

Query: 421 TLLQKMGFGMLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQL 480
           T LQ++G G+ + I +M VSA VEQ RR +ALT+P L    A R+GAISSMS +WL+PQL
Sbjct: 423 TQLQRVGAGLFLCITSMMVSAIVEQYRRKVALTKPTL--GLAPRKGAISSMSGMWLIPQL 482

Query: 481 TLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLT---- 540
            L+G+++A   + Q+EFYYK+FPENMRS  GSL + G+ L++YLS F+++ VH  T    
Sbjct: 483 VLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYYCGIGLASYLSTFLLSAVHDTTEGFS 542

Query: 541 AGHWLPEDLNQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYKGSGSHGVDEMDFGKTEFE 578
            G WLPEDLN+GRL+YFYFLV+ +  +NL YF++ S WY+YK   +   D MD    EF+
Sbjct: 543 GGSWLPEDLNKGRLEYFYFLVAGMMTLNLAYFLLVSHWYRYKDVVAKDKD-MDKTSAEFD 584

BLAST of Tan0018230 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 461.8 bits (1187), Expect = 7.9e-130
Identity = 254/573 (44.33%), Postives = 363/573 (63.35%), Query Frame = 0

Query: 13  LDEPKVNYR--GVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNITATTIMNIFH 72
           LD  KV  +  G +A+ F++GNET E+LG+ G+ +N +VYLT VFH++ + A  ++NI+ 
Sbjct: 43  LDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWS 102

Query: 73  GSTNFSTLFGAFLCDTYFGRYKTLGYASIASFLGMGT------------GGCLVE----- 132
           G TN + L GA++ DTY GR+KT+ +AS A+ LG+ T              C  +     
Sbjct: 103 GFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSC 162

Query: 133 --PTPWQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFTFT 192
             P   Q+  LL GL  + +G+GGIRPC++ FG DQF+  T  G  G+ SFFNWYY TFT
Sbjct: 163 GGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFT 222

Query: 193 FAMMISLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEPHGSPFTSVMRV 252
             ++I+ T++VY+Q +VSW  G +IP  LM L+  +FF G   YV ++P GS F+ + +V
Sbjct: 223 VVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQV 282

Query: 253 LVAAFKKRRLPLPDHQWPSLFNYMPS--NSINSNLPYTHQFSFLDKAAIITEEDKLNSDG 312
           +VAA KKR+L LP     ++  Y P+  +S+ S L  ++QF  LDKAA++ E D L  +G
Sbjct: 283 IVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEGD-LTPEG 342

Query: 313 SAADPWTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQSDRRLIIFGN 372
             AD W LCS Q+VEEVKCL+R++PIW+A II   A T Q T+ V QAL+ DR L     
Sbjct: 343 PPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNL----- 402

Query: 373 TTSSTTTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGITLLQKMGFG 432
                     F+IPA S ++ +++ + I++PFYDR+ VP +RRIT  + GITLLQ++G G
Sbjct: 403 -------GPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTG 462

Query: 433 MLIAILTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQLTLIGLSEAF 492
           ++ AI +M V+  VE+ RR  ++         AG    ++ MS  WL PQL L+GL EAF
Sbjct: 463 IVFAIFSMIVAGIVERMRRIRSIN--------AGDPTGMTPMSVFWLSPQLILMGLCEAF 522

Query: 493 TVIAQVEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQLTAGH----WLPEDL 552
            +I Q+EF+  +FPE+MRSI  SL  L  A S+YLS F+VTVVH+ + GH    WL ++L
Sbjct: 523 NIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNL 582

Query: 553 NQGRLDYFYFLVSALEVVNLGYFVVFSKWYKYK 559
           N G+LDYFY+L++ L VVNL YF   ++ Y+YK
Sbjct: 583 NAGKLDYFYYLIAVLGVVNLVYFWYCARGYRYK 594

BLAST of Tan0018230 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 432.2 bits (1110), Expect = 6.7e-121
Identity = 248/568 (43.66%), Postives = 345/568 (60.74%), Query Frame = 0

Query: 16  PKVNYRGVKAMPFVVGNETFEKLGTTGISSNLLVYLTDVFHMKNITATTIMNIFHGSTNF 75
           P+    G +A+ F++GNET EKLG+ G+S+N ++YL +VFHM+ + A  +  ++ G TNF
Sbjct: 11  PEKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNF 70

Query: 76  STLFGAFLCDTYFGRYKTLGYASIASFLGMGT---GGCLV----------------EPTP 135
           + L GA + D Y GR+KT+ YAS+ S LG+ T     CL                 +P  
Sbjct: 71  APLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNK 130

Query: 136 WQLAFLLFGLGLVIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFTFTFAMMI 195
            QL  L  GLG + IG+GGIRPC++ FG DQF+  T  G  G+ SFFNWYY T T  ++ 
Sbjct: 131 LQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIF 190

Query: 196 SLTIIVYVQTEVSWGWGLAIPAFLMFLSCALFFMGSWIYVKLEPHGSPFTSVMRVLVAAF 255
           S T++VY+QT VSW  G +IP  LM  +  LFF+G   YV ++P GS F+ + RV+VAA 
Sbjct: 191 SHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAAR 250

Query: 256 KKR--RLPLPDHQWPSLFNYMPSNSINSNLPYTHQFSFLDKAAIITEEDKLNSDGSAADP 315
           KKR  ++ L D      +       + S LP T QF FLDKAA+I + D L S+G  A+ 
Sbjct: 251 KKRDLKISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDGD-LTSEGVPANK 310

Query: 316 WTLCSTQQVEEVKCLVRVIPIWAAAIIYHVATTQQQTYVVFQALQSDRRLIIFGNTTSST 375
           W LCS Q+VEEVKCL+RV+P+W+A II  VA T Q T++VFQA + DR +          
Sbjct: 311 WRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHM---------- 370

Query: 376 TTIHNFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAKEGGITLLQKMGFGMLIAI 435
               +F+IPAAS T+ + I + IW+P Y+ +LVP L R+  ++  +TLLQ+MG G++ AI
Sbjct: 371 --GPHFEIPAASITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAI 430

Query: 436 LTMFVSAFVEQKRRNLALTQPLLCQEAAGRRGAISSMSALWLVPQLTLIGLSEAFTVIAQ 495
           L+MF + FVE  RR               R   ++ MS  WL   L L+GL E+F  I  
Sbjct: 431 LSMFTAGFVEGVRRT--------------RATEMTQMSVFWLALPLILMGLCESFNFIGL 490

Query: 496 VEFYYKEFPENMRSIGGSLSFLGLALSNYLSGFMVTVVHQL--TAGH--WLPEDLNQGRL 555
           +EF+  +FPE+MRSI  SL  L  A +NYLS  +VT VH++  T  H  WL +DL++G+L
Sbjct: 491 IEFFNSQFPEHMRSIANSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKL 548

Query: 556 DYFYFLVSALEVVNLGYFVVFSKWYKYK 559
           DYFY+L++ L VVNL YF   +  Y+YK
Sbjct: 551 DYFYYLIAVLGVVNLVYFWYCAHRYQYK 548