Tan0018166 (gene) Snake gourd v1

Overview
NameTan0018166
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionchitinase-3-like protein 2
LocationLG08: 2904316 .. 2905850 (-)
RNA-Seq ExpressionTan0018166
SyntenyTan0018166
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAACAATAATTATGTGAATGCTGGCTATTGGTTCACCCACACCAACAACCACCTTCCCATTTCCGAAATCGATACAAATCTTTTCACTCATCTCTTTTGTGGTTATGTTGGTCTCAACTCCCAAACTTATGAACTCTCGATTTCCGATTCAGCAAAGCCCCTTATTGATCAATTTTCTCGAAAAATCAAAGAAAACAACTCCGAGATGAAAACTCTATTGTCCATTGGAGGAGATGATGCAAAAGCGGATAGATTCGCAACCATGGCTGCCAATTCCTCAAATCGTGCATCGTTTATTAGAAGCTTCATTTCTAAAGCAAGAGAGGGTAATTTTGATGGTGTGGATCTTCAGTGGTTGTACCCTTCTTCTGTGGAGGATATGACCAATTTTGAAGCTCTCGTTATTGAGTGTTACAATGGTGTTGTCAAAGAAGCTCAAAAATCTCATGAACCACGATTGATTCTTGTTGCTACAGTCTTCTATTCACCTTATATAAAGAACATTAAGTATCCAATTCAAGTAATTCGAAGAACTTTGGATTGGATTAACATAATTGCTTATGATATTTACACTCCCACTTCGTCGAGTTTGGAAACTGGACCATCCTCAGCATTCTACAATCCAAAAAGAAACTCCTTGTCTGGATACTTTGGTATGTTTTATAAGGTTTAAGTTAAGTATTCTAGATTCTATTGTTTTAATAGTGTTGGCAGTGTGGCCAAAGTCCCACATCGACTAGATAAGAGAAAAATCATGGGTATATAAGTTAGAGATATTATCTCTATTGGTATGAAGAAAAAACAAAGCCATGAAGGCATATGTACAAAGTGAACAATATCAATCTAATGTGGAGATATGTAGACGCAAAGAAGTTAAAATAACTACAAAAATGCCCTTGACTAAAATTTACTTAAATGCCCTTTTTTTTTTTAATTTTTTGTCACTTTCTCACAAATATTTTCATTATAATGCTCCTTAACCTTACTCAATAAGGGTTTAGTTATTAATCATGTTTATACATGATCCATATGAAATATTGTTTGTGTTGAATTATAATTTCAGGTATGAGAACATGGATTCAATTAGGTTTGCCTTCTAAAATGATGGTATTTGGACTTCCCTTTCATGGTTGGGCATGGAAACTTAAGAATTGGAAGAAACATGAGGTGTTTTCAGAAGCCCAAGGAGCAGCTAGAGGAGAAAATATATGCATGGAAGGGTTGATTGAGTATAGGAATATTAAGAAATTTATAGCTGATAACGATGCCAATAATGTTCTCATAGAACGAAAATTTGTAATTGGTTACACATATTGTGGCTCAACTTGGATTGCCTATGAAGATGAAGACACCATCAAATTCAAGATTTCAACACTTAAACGTGACTTCTCATTGAATGGTTACTTTGTTTTCAACATTGCAGCTGATGATGAAAATCATTCTCTTGCCAAGGCAGCCTCAAGAGAATGGAAAAAAGGCCATCCCGACTTTTGTTGTTGCTTATTTGATTTTCTATCTCTTTGTTATTAA

mRNA sequence

ATGGCCAACAATAATTATGTGAATGCTGGCTATTGGTTCACCCACACCAACAACCACCTTCCCATTTCCGAAATCGATACAAATCTTTTCACTCATCTCTTTTGTGGTTATGTTGGTCTCAACTCCCAAACTTATGAACTCTCGATTTCCGATTCAGCAAAGCCCCTTATTGATCAATTTTCTCGAAAAATCAAAGAAAACAACTCCGAGATGAAAACTCTATTGTCCATTGGAGGAGATGATGCAAAAGCGGATAGATTCGCAACCATGGCTGCCAATTCCTCAAATCGTGCATCGTTTATTAGAAGCTTCATTTCTAAAGCAAGAGAGGGTAATTTTGATGGTGTGGATCTTCAGTGGTTGTACCCTTCTTCTGTGGAGGATATGACCAATTTTGAAGCTCTCGTTATTGAGTGTTACAATGGTGTTGTCAAAGAAGCTCAAAAATCTCATGAACCACGATTGATTCTTGTTGCTACAGTCTTCTATTCACCTTATATAAAGAACATTAAGTATCCAATTCAAGTAATTCGAAGAACTTTGGATTGGATTAACATAATTGCTTATGATATTTACACTCCCACTTCGTCGAGTTTGGAAACTGGACCATCCTCAGCATTCTACAATCCAAAAAGAAACTCCTTGTCTGGATACTTTGGTATGAGAACATGGATTCAATTAGGTTTGCCTTCTAAAATGATGGTATTTGGACTTCCCTTTCATGGTTGGGCATGGAAACTTAAGAATTGGAAGAAACATGAGGTGTTTTCAGAAGCCCAAGGAGCAGCTAGAGGAGAAAATATATGCATGGAAGGGTTGATTGAGTATAGGAATATTAAGAAATTTATAGCTGATAACGATGCCAATAATGTTCTCATAGAACGAAAATTTGTAATTGGTTACACATATTGTGGCTCAACTTGGATTGCCTATGAAGATGAAGACACCATCAAATTCAAGATTTCAACACTTAAACGTGACTTCTCATTGAATGGTTACTTTGTTTTCAACATTGCAGCTGATGATGAAAATCATTCTCTTGCCAAGGCAGCCTCAAGAGAATGGAAAAAAGGCCATCCCGACTTTTGTTGTTGCTTATTTGATTTTCTATCTCTTTGTTATTAA

Coding sequence (CDS)

ATGGCCAACAATAATTATGTGAATGCTGGCTATTGGTTCACCCACACCAACAACCACCTTCCCATTTCCGAAATCGATACAAATCTTTTCACTCATCTCTTTTGTGGTTATGTTGGTCTCAACTCCCAAACTTATGAACTCTCGATTTCCGATTCAGCAAAGCCCCTTATTGATCAATTTTCTCGAAAAATCAAAGAAAACAACTCCGAGATGAAAACTCTATTGTCCATTGGAGGAGATGATGCAAAAGCGGATAGATTCGCAACCATGGCTGCCAATTCCTCAAATCGTGCATCGTTTATTAGAAGCTTCATTTCTAAAGCAAGAGAGGGTAATTTTGATGGTGTGGATCTTCAGTGGTTGTACCCTTCTTCTGTGGAGGATATGACCAATTTTGAAGCTCTCGTTATTGAGTGTTACAATGGTGTTGTCAAAGAAGCTCAAAAATCTCATGAACCACGATTGATTCTTGTTGCTACAGTCTTCTATTCACCTTATATAAAGAACATTAAGTATCCAATTCAAGTAATTCGAAGAACTTTGGATTGGATTAACATAATTGCTTATGATATTTACACTCCCACTTCGTCGAGTTTGGAAACTGGACCATCCTCAGCATTCTACAATCCAAAAAGAAACTCCTTGTCTGGATACTTTGGTATGAGAACATGGATTCAATTAGGTTTGCCTTCTAAAATGATGGTATTTGGACTTCCCTTTCATGGTTGGGCATGGAAACTTAAGAATTGGAAGAAACATGAGGTGTTTTCAGAAGCCCAAGGAGCAGCTAGAGGAGAAAATATATGCATGGAAGGGTTGATTGAGTATAGGAATATTAAGAAATTTATAGCTGATAACGATGCCAATAATGTTCTCATAGAACGAAAATTTGTAATTGGTTACACATATTGTGGCTCAACTTGGATTGCCTATGAAGATGAAGACACCATCAAATTCAAGATTTCAACACTTAAACGTGACTTCTCATTGAATGGTTACTTTGTTTTCAACATTGCAGCTGATGATGAAAATCATTCTCTTGCCAAGGCAGCCTCAAGAGAATGGAAAAAAGGCCATCCCGACTTTTGTTGTTGCTTATTTGATTTTCTATCTCTTTGTTATTAA

Protein sequence

MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQFSRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQWLYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRTLDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPFHGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIGYTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWKKGHPDFCCCLFDFLSLCY
Homology
BLAST of Tan0018166 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.7e-75
Identity = 151/350 (43.14%), Postives = 214/350 (61.14%), Query Frame = 0

Query: 7   VNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQFSRKIKE 66
           V A YWF    +  P+++ID++LFTHLFC +  LNSQT ++++S + +P    F++ ++ 
Sbjct: 28  VKASYWF--PASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 67  NNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQWLYPSSV 126
            N  +KTLLSIGG  A    +A+MA+N ++R SFI S I  AR   F G+DL W YPSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 127 EDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRTLDWINI 186
            +MTNF  L+ E  + VV EA  S +PRL+L A VFYS    ++ YP+  +  +LDW+N+
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYYSVLYPVSAVASSLDWVNL 207

Query: 187 IAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPFHGWAWK 246
           +AYD Y P  S + TGP +A ++P     SG  G R+WIQ GLP+K  V G P++G+AW+
Sbjct: 208 MAYDFYGPGWSRV-TGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVLGFPYYGYAWR 267

Query: 247 LKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIG-YTYCG 306
           L N   H  ++   GAA    I  +G I Y  I+KFI DN A  V      V+G Y Y G
Sbjct: 268 LTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGATTVY--NSTVVGDYCYAG 327

Query: 307 STWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREW 356
           + WI Y+D  +I  K+   K+   L GYF +++ ADD N  L++AAS+ W
Sbjct: 328 TNWIGYDDNQSIVTKVRYAKQR-GLLGYFSWHVGADD-NSGLSRAASQAW 366

BLAST of Tan0018166 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 200.7 bits (509), Expect = 3.0e-50
Identity = 125/354 (35.31%), Postives = 196/354 (55.37%), Query Frame = 0

Query: 7   VNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQFSRKIKE 66
           V A YW   ++     S IDTN FTH++  +V    +++ L I++S K    ++   I  
Sbjct: 55  VRAAYWPAWSD--FSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYD-GIHN 114

Query: 67  NNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQWLYPSSV 126
               + TLLSIGG    A  F+ MA++  NRASFI S I  AR+  F+G+DL W +P   
Sbjct: 115 IRPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDE 174

Query: 127 EDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNI-----KYPIQVIRRTL 186
           +DM+N   L+ E Y  +V EA  S + RL+L + V+++  I  I      YP++ IR+ L
Sbjct: 175 KDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIRKYL 234

Query: 187 DWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPFH 246
           DW + + +D     ++  ETG ++A Y+P  N +S  +G+ +WI  G+P++ +V GLP +
Sbjct: 235 DWASPMCFDYNGAWAN--ETGFNAALYDPNSN-ISTKYGIGSWIGSGVPAEKLVMGLPLY 294

Query: 247 GWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIGY 306
           G AW+LK+   H V ++A G A    +  +G ++Y  I  F  D  A  V+ +   V  Y
Sbjct: 295 GRAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDEILVFNKDTGA-KVVYDEVAVSFY 354

Query: 307 TYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREW 356
           +Y G+TWI Y+D  +I  K+    R   L GYF + I   D++ +++K AS  W
Sbjct: 355 SYSGTTWIGYDDGPSITKKVQ-FARSMGLKGYFFWAI-GKDKDWTISKQASNAW 395

BLAST of Tan0018166 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 3.1e-47
Identity = 121/362 (33.43%), Postives = 193/362 (53.31%), Query Frame = 0

Query: 4   NNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQFSRK 63
           N+ V + YW     +    S IDTN FTH+   ++     +++L I+ S       F + 
Sbjct: 37  NHGVRSAYW--PAGDDFSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKA 96

Query: 64  IKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQWLYP 123
           ++  +  +KTLLSIGG  + +  F+ +A+   NR  FI S I  AR+  FDGVDL W +P
Sbjct: 97  LRHRSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFP 156

Query: 124 SSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNI------KYPIQVI 183
            + +DM N   L  E YN +  EA+   +PRL+L + V+Y+  ++ I       YP Q I
Sbjct: 157 ETQQDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAI 216

Query: 184 RRTLDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFG 243
            + LDW + + +D +    ++  T  ++A Y+ K + +S  FG+ +WI+ G+  + +V G
Sbjct: 217 NKYLDWASPMCFDYHGTWDNN--TDFNAALYDSK-SEISTNFGLHSWIKSGVRPEKLVMG 276

Query: 244 LPFHGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKF 303
           L  +G AW+LK+   + V +EA G A       +G + Y  I KF   + A NV+ ++  
Sbjct: 277 LALYGRAWELKDPNVNGVGAEAVGPATD----TDGSMNYNEILKFNKQSGA-NVVYDKVA 336

Query: 304 VIGYTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWKK 360
           +  Y+Y G+TWI Y+D  +I  K+   K    L GYF F     D++ S++K AS  W  
Sbjct: 337 ISFYSYAGTTWIGYDDGPSITTKVRFAK-SLGLKGYF-FWALGKDKDWSISKQASNAW-- 384

BLAST of Tan0018166 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.9e-45
Identity = 109/350 (31.14%), Postives = 182/350 (52.00%), Query Frame = 0

Query: 11  YWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQFSRKIKENNSE 70
           YW    N   P + +DT+LFTH+F  ++  N+ TY+L IS S    ++ F++  K  +  
Sbjct: 39  YWL--ENPFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKTKSPP 98

Query: 71  MKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQWLYPSSVEDMT 130
             TL SIGG  + +   A +A++    A+FI S I  AR   FDG+DL W +P + ++M 
Sbjct: 99  AATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNTKEMN 158

Query: 131 NFEALVIECYNGVVKEAQKSHEPRLILVATVF----YSPYIKNIKYPIQVIRRTLDWINI 190
           +   ++ +    +  E   +  P L+L A V+    +S Y +   YP+  I   LDW+N+
Sbjct: 159 DLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLDWVNV 218

Query: 191 IAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPFHGWAWK 250
           ++Y++  P S+  +TG  S  ++PK N +S   G+ +WI  G+  + +V G+P +G +WK
Sbjct: 219 MSYELRGPRSN--KTGAPSGTFDPKSN-VSVVSGLLSWIHSGVVPEKLVMGMPLYGKSWK 278

Query: 251 LKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIGYTYCGS 310
           L++   H + + + G+  G N    GL+ Y  +  F     A  V  +      Y+Y GS
Sbjct: 279 LRDPNVHGIGAPSVGSGPGVN----GLMAYFQVLDFNRQKSA-KVEYDVDTASVYSYSGS 338

Query: 311 TWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWK 357
           TWI Y++  T+  K+    +   L GYF +    D  +  +A  AS+ WK
Sbjct: 339 TWIGYDNPFTVSIKVG-FAQALKLRGYFFWVAGLDTLDWKIATQASKAWK 377

BLAST of Tan0018166 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 1.9e-41
Identity = 110/354 (31.07%), Postives = 180/354 (50.85%), Query Frame = 0

Query: 11  YWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYEL----SISDSAKPLIDQFSRKIKE 70
           YW    N   P S IDT+LFTH+F  +V  N  TY+L      S +    +  F+   K 
Sbjct: 41  YWI--ENPLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTTFTNTFKT 100

Query: 71  NNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQWLYPSSV 130
               + TLLSIGG  + +  FA +A++ + RA+FI S I  AR   FDG+D  W +P++ 
Sbjct: 101 KTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFDWEFPTTT 160

Query: 131 EDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYI----KNIKYPIQVIRRTLD 190
           ++M +   L+ +    +  E   +  P L+L A V+++       +   YP+  I + LD
Sbjct: 161 KEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVDSINKNLD 220

Query: 191 WINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPFHG 250
           W+N+++YD+    S S  TG  S  ++ K N +S   G+ +WI+ G+  + +V G+P +G
Sbjct: 221 WVNVMSYDL--RGSGSNVTGAPSGMFDSKSN-VSVVSGLFSWIRGGVAPEKIVMGMPLYG 280

Query: 251 WAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIGYT 310
            +WKL++   H + +   G   G    ++G + Y  +  F     A  V+ +++    Y+
Sbjct: 281 KSWKLQDPNVHGIGAPNVGPGPG----VDGGMAYFQVVDFNKQMGA-KVVYDKETGSVYS 340

Query: 311 YCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWK 357
           Y GSTWI Y+D  T+  K+    +   L GYF F  A  D +      AS+ W+
Sbjct: 341 YSGSTWIGYDDPFTVSVKVG-FAQALKLGGYF-FWAAGYDTSDWKVSTASKAWR 382

BLAST of Tan0018166 vs. NCBI nr
Match: XP_023545460.1 (chitinase-3-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 607.8 bits (1566), Expect = 6.2e-170
Identity = 292/366 (79.78%), Postives = 326/366 (89.07%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQF 60
           MA NN VNAGYWFT TN HLPIS+IDTNL+THLFCGY  LNSQTYELS SDSAKPLI  F
Sbjct: 1   MAKNNIVNAGYWFTQTNAHLPISKIDTNLYTHLFCGYANLNSQTYELSFSDSAKPLIHHF 60

Query: 61  SRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQW 120
           S  +KENNS++KTLLSIGGDDA+A+ FA MAA SSNRASFI SFI KAREGNFDGVD+QW
Sbjct: 61  SSTVKENNSKVKTLLSIGGDDARAESFAAMAATSSNRASFIGSFICKAREGNFDGVDVQW 120

Query: 121 LYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRT 180
           +YPS VEDM+NFEAL+IEC NGVVKEA++S +PRLILVAT+FYSPYI +++YPIQVIRRT
Sbjct: 121 VYPSCVEDMSNFEALIIECRNGVVKEAKESRQPRLILVATLFYSPYINDVRYPIQVIRRT 180

Query: 181 LDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPF 240
           LDW+N+IAYDIYTPTSS+ ETGPSSAFYNP +NS SGYFG+RTWIQ GLPSKMMVFGLPF
Sbjct: 181 LDWVNVIAYDIYTPTSSTNETGPSSAFYNPNKNSSSGYFGIRTWIQQGLPSKMMVFGLPF 240

Query: 241 HGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIG 300
           HGWAW+L++ KKH+VFS AQG ARG+NI MEGLIEYRNIKKFIADNDANNV ++R FVIG
Sbjct: 241 HGWAWELESSKKHKVFSPAQGPARGQNISMEGLIEYRNIKKFIADNDANNVPMDRNFVIG 300

Query: 301 YTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWKKGHP 360
           YT  GSTWIAYEDEDTIKFKIS +K +  LNGYFV+NIAADDE+HSLAK ASREWKKG  
Sbjct: 301 YTNFGSTWIAYEDEDTIKFKISRVKDNLGLNGYFVWNIAADDEHHSLAKVASREWKKGQ- 360

Query: 361 DFCCCL 367
            FCCCL
Sbjct: 361 GFCCCL 365

BLAST of Tan0018166 vs. NCBI nr
Match: XP_022999353.1 (chitinase-3-like protein 2 [Cucurbita maxima])

HSP 1 Score: 599.7 bits (1545), Expect = 1.7e-167
Identity = 289/366 (78.96%), Postives = 321/366 (87.70%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQF 60
           MA NN VNAGYWFT TN H PIS+ID+NL+THLFCGY  LNSQTYELS SD+AKPLI  F
Sbjct: 1   MAKNNIVNAGYWFTQTNAHFPISKIDSNLYTHLFCGYANLNSQTYELSFSDTAKPLIHHF 60

Query: 61  SRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQW 120
           S  +KENNS++KTLLSIGGD A AD FA MAA+SSNRASFI SFI KAREGNFDGVD+QW
Sbjct: 61  SNAVKENNSKVKTLLSIGGDHASADSFAAMAASSSNRASFIGSFICKAREGNFDGVDVQW 120

Query: 121 LYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRT 180
           +YPS VEDM+NFEAL+IEC+NGVVKEA++S  PRLILVATVFYSPYI +++YPIQVIRR 
Sbjct: 121 VYPSCVEDMSNFEALIIECHNGVVKEAKESRHPRLILVATVFYSPYIHDVRYPIQVIRRA 180

Query: 181 LDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPF 240
           LDW+N+IA+DIYTPTSS  ETGPSSAFYNP+RNS SGYFG+RTWIQ GLPSKMMVFGLPF
Sbjct: 181 LDWVNVIAFDIYTPTSSPNETGPSSAFYNPERNSSSGYFGIRTWIQQGLPSKMMVFGLPF 240

Query: 241 HGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIG 300
           HGWAWKL++ KKH VFS AQG A G+NI MEGLIEYRNIKKFIADNDANNV ++R FVIG
Sbjct: 241 HGWAWKLESSKKHNVFSPAQGPAGGQNISMEGLIEYRNIKKFIADNDANNVPMDRSFVIG 300

Query: 301 YTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWKKGHP 360
           YT  GSTWIAYEDEDTIKFKIS +K +  LNGYFV+NIAADDE+HSLAK ASREWKKG  
Sbjct: 301 YTNFGSTWIAYEDEDTIKFKISRVKTNLGLNGYFVWNIAADDEHHSLAKLASREWKKGQ- 360

Query: 361 DFCCCL 367
            FCCCL
Sbjct: 361 GFCCCL 365

BLAST of Tan0018166 vs. NCBI nr
Match: KAG6599436.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 596.7 bits (1537), Expect = 1.4e-166
Identity = 287/366 (78.42%), Postives = 322/366 (87.98%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQF 60
           MA NN VNAGYWFT TN H PIS+IDTNL+THLFCGY  LNSQTYELS SDSAKPLI  F
Sbjct: 1   MAKNNIVNAGYWFTQTNAHFPISKIDTNLYTHLFCGYANLNSQTYELSFSDSAKPLIRHF 60

Query: 61  SRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQW 120
           S  +KENNS++KTLLSIGGD A A+ FA MAA SSNRASFI SFI KAREGNFDGVD+QW
Sbjct: 61  SNAVKENNSKVKTLLSIGGDHASAESFAAMAATSSNRASFIGSFICKAREGNFDGVDVQW 120

Query: 121 LYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRT 180
           LYPS  EDM+NFEAL+IEC+NGVV+EA++S +PRLILVATVFYSPYI +++YPIQVIR  
Sbjct: 121 LYPSCAEDMSNFEALIIECHNGVVQEAKESRQPRLILVATVFYSPYINDVRYPIQVIRGA 180

Query: 181 LDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPF 240
           LDW+N+IAYDIYTPTSS+ ETGPSSAFYNP+RNS SGYFG+RTWIQ GLPSKM+VFGLPF
Sbjct: 181 LDWVNVIAYDIYTPTSSTNETGPSSAFYNPERNSSSGYFGIRTWIQQGLPSKMIVFGLPF 240

Query: 241 HGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIG 300
           HGWAWKL++ KKH+VFS AQG ARG+NI MEGLIEY NIKKFIADNDANNV ++R FVIG
Sbjct: 241 HGWAWKLESSKKHKVFSPAQGPARGQNISMEGLIEYGNIKKFIADNDANNVPMDRNFVIG 300

Query: 301 YTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWKKGHP 360
           YT  GSTWIAYEDEDTIKFKIS++K +  L+GYFV+NIAADDE+HSLAK ASREWKKG  
Sbjct: 301 YTNFGSTWIAYEDEDTIKFKISSVKTNLGLSGYFVWNIAADDEHHSLAKLASREWKKGQ- 360

Query: 361 DFCCCL 367
            FCCCL
Sbjct: 361 GFCCCL 365

BLAST of Tan0018166 vs. NCBI nr
Match: KAG7030351.1 (Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 595.5 bits (1534), Expect = 3.2e-166
Identity = 287/366 (78.42%), Postives = 320/366 (87.43%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQF 60
           MA NN VNAGYWFT TN H PIS+IDTNL+THLFCGY  LNSQTYELS SDSAKPLI  F
Sbjct: 1   MAKNNIVNAGYWFTQTNAHFPISKIDTNLYTHLFCGYANLNSQTYELSFSDSAKPLIRHF 60

Query: 61  SRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQW 120
           S  +KENNS++KTLLSIGGD A A+ FA MAA SSNRASFI SFI KAREGNFDGVD+QW
Sbjct: 61  SNAVKENNSKVKTLLSIGGDHASAESFAAMAATSSNRASFIGSFICKAREGNFDGVDVQW 120

Query: 121 LYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRT 180
           LYPS  EDM+NFEAL+IEC+NGVV+EA++S +PRLILVATVFYSPYI +++YPIQVIR  
Sbjct: 121 LYPSCAEDMSNFEALIIECHNGVVQEAKESRQPRLILVATVFYSPYINDVRYPIQVIRGA 180

Query: 181 LDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPF 240
           LDW+N+IAYDIYTPTSS+ ETGPSSAFYNP+RNS SGYFG+RTWIQ GLPSKM+VFGLPF
Sbjct: 181 LDWVNVIAYDIYTPTSSTNETGPSSAFYNPERNSSSGYFGIRTWIQPGLPSKMIVFGLPF 240

Query: 241 HGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIG 300
           HGWAWKL++ KKH+VFS AQG ARG+NI MEGLIEY NIKKFIADNDANNV ++R FVIG
Sbjct: 241 HGWAWKLESSKKHKVFSPAQGPARGQNISMEGLIEYGNIKKFIADNDANNVPMDRSFVIG 300

Query: 301 YTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWKKGHP 360
           YT  GSTWIAYEDEDTIK KIS +K +  LNGYFV+NIAADDE+HSLAK ASREWKKG  
Sbjct: 301 YTNFGSTWIAYEDEDTIKLKISRVKTNLGLNGYFVWNIAADDEHHSLAKVASREWKKGQ- 360

Query: 361 DFCCCL 367
            FCCCL
Sbjct: 361 GFCCCL 365

BLAST of Tan0018166 vs. NCBI nr
Match: KAG6599363.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 570.9 bits (1470), Expect = 8.3e-159
Identity = 275/349 (78.80%), Postives = 307/349 (87.97%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQF 60
           MA NN VNAGYWFT TN H PIS+IDTNL+THLFCGY  LNSQTYELS SDSAKPLI  F
Sbjct: 1   MAKNNIVNAGYWFTQTNAHCPISKIDTNLYTHLFCGYANLNSQTYELSFSDSAKPLIRHF 60

Query: 61  SRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQW 120
           S  +KENNS++KTLLSIGGD A AD FA MAA SSNRASFI SFI KAREGNFDGVD+QW
Sbjct: 61  SNAVKENNSKVKTLLSIGGDHASADSFAAMAATSSNRASFIGSFICKAREGNFDGVDVQW 120

Query: 121 LYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRT 180
           LYPS  EDM+NFEAL+IEC+NGVV+EA++S +PRLILVATVFYSPYI +++YPIQVIR  
Sbjct: 121 LYPSCAEDMSNFEALIIECHNGVVQEAKESRQPRLILVATVFYSPYINDVRYPIQVIRGA 180

Query: 181 LDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPF 240
           LDW+N+IAYDIYTPTSS+ ETGPSSAFYNP+RNS SGYFG+RTWIQ GLPSKM+VFGLPF
Sbjct: 181 LDWVNVIAYDIYTPTSSTNETGPSSAFYNPERNSSSGYFGIRTWIQQGLPSKMIVFGLPF 240

Query: 241 HGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIG 300
           HGWAWKL++ KKH+VFS AQG ARG+NI MEGLIEY NIKKFIADNDANNV ++R FVIG
Sbjct: 241 HGWAWKLESSKKHKVFSPAQGPARGQNISMEGLIEYGNIKKFIADNDANNVPMDRSFVIG 300

Query: 301 YTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAK 350
           YT  GSTWIAYEDEDTIK KIS +K +  LNGYFV+NIAADDE+HSLAK
Sbjct: 301 YTNFGSTWIAYEDEDTIKLKISRVKTNLGLNGYFVWNIAADDEHHSLAK 349

BLAST of Tan0018166 vs. ExPASy TrEMBL
Match: A0A6J1KJH1 (chitinase-3-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111493752 PE=4 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 8.1e-168
Identity = 289/366 (78.96%), Postives = 321/366 (87.70%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQF 60
           MA NN VNAGYWFT TN H PIS+ID+NL+THLFCGY  LNSQTYELS SD+AKPLI  F
Sbjct: 1   MAKNNIVNAGYWFTQTNAHFPISKIDSNLYTHLFCGYANLNSQTYELSFSDTAKPLIHHF 60

Query: 61  SRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQW 120
           S  +KENNS++KTLLSIGGD A AD FA MAA+SSNRASFI SFI KAREGNFDGVD+QW
Sbjct: 61  SNAVKENNSKVKTLLSIGGDHASADSFAAMAASSSNRASFIGSFICKAREGNFDGVDVQW 120

Query: 121 LYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRT 180
           +YPS VEDM+NFEAL+IEC+NGVVKEA++S  PRLILVATVFYSPYI +++YPIQVIRR 
Sbjct: 121 VYPSCVEDMSNFEALIIECHNGVVKEAKESRHPRLILVATVFYSPYIHDVRYPIQVIRRA 180

Query: 181 LDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPF 240
           LDW+N+IA+DIYTPTSS  ETGPSSAFYNP+RNS SGYFG+RTWIQ GLPSKMMVFGLPF
Sbjct: 181 LDWVNVIAFDIYTPTSSPNETGPSSAFYNPERNSSSGYFGIRTWIQQGLPSKMMVFGLPF 240

Query: 241 HGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIG 300
           HGWAWKL++ KKH VFS AQG A G+NI MEGLIEYRNIKKFIADNDANNV ++R FVIG
Sbjct: 241 HGWAWKLESSKKHNVFSPAQGPAGGQNISMEGLIEYRNIKKFIADNDANNVPMDRSFVIG 300

Query: 301 YTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWKKGHP 360
           YT  GSTWIAYEDEDTIKFKIS +K +  LNGYFV+NIAADDE+HSLAK ASREWKKG  
Sbjct: 301 YTNFGSTWIAYEDEDTIKFKISRVKTNLGLNGYFVWNIAADDEHHSLAKLASREWKKGQ- 360

Query: 361 DFCCCL 367
            FCCCL
Sbjct: 361 GFCCCL 365

BLAST of Tan0018166 vs. ExPASy TrEMBL
Match: A0A6J1CQ34 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013117 PE=4 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 6.9e-151
Identity = 256/357 (71.71%), Postives = 309/357 (86.55%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQF 60
           MA NN+VNAGYWFTHT++ LP S ID NL+THLFCG+ GLNSQTYELSISDSAKPLI  F
Sbjct: 1   MACNNFVNAGYWFTHTSSQLPASRIDANLYTHLFCGHAGLNSQTYELSISDSAKPLIHHF 60

Query: 61  SRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQW 120
           S+ +KENNSE+KTLLSIGGDDAKA+RF  MAA+SS+RA+FIRS ISKAREGNFDG+DLQW
Sbjct: 61  SQTVKENNSELKTLLSIGGDDAKAERFGAMAAHSSHRAAFIRSSISKAREGNFDGLDLQW 120

Query: 121 LYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRT 180
           LYPSSVEDM NFEAL+IEC+  +++E+++S EPRLI VATVFYSP ++N+ YPIQ IRRT
Sbjct: 121 LYPSSVEDMANFEALIIECHRAIIRESRESKEPRLIFVATVFYSPRLENLTYPIQAIRRT 180

Query: 181 LDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPF 240
           LDW+N+IAYDI+TP  SS E GPSSAF+NP+R S SG FG+RTWI+LGLPSKMMVFG+PF
Sbjct: 181 LDWVNVIAYDIFTPILSSNEVGPSSAFFNPRRRSSSGDFGIRTWIRLGLPSKMMVFGVPF 240

Query: 241 HGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIG 300
           HGWAWKL       VF+ AQGAA+G++I MEGLIEYRN+KKFIA+NDANNV+++++F I 
Sbjct: 241 HGWAWKLNCSGNPRVFAPAQGAAQGQDISMEGLIEYRNVKKFIAENDANNVVLDQRFAIA 300

Query: 301 YTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWKK 358
           YT+C ST IAYEDEDTI FKIS +K++  + GYF++NI+ADDE+ SLA+AAS EW K
Sbjct: 301 YTHCDSTGIAYEDEDTISFKISRVKKNLEMLGYFLWNISADDEDQSLARAASTEWIK 357

BLAST of Tan0018166 vs. ExPASy TrEMBL
Match: A0A6J1CQ39 (oviduct-specific glycoprotein-like OS=Momordica charantia OX=3673 GN=LOC111013129 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.3e-144
Identity = 244/356 (68.54%), Postives = 298/356 (83.71%), Query Frame = 0

Query: 2   ANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQFS 61
           +N+ +VNAGYWFTHTN   P S+ID NLFTHLFC + GLNSQTYELSISDS K LI +FS
Sbjct: 3   SNDLFVNAGYWFTHTNTQFPASKIDANLFTHLFCAHAGLNSQTYELSISDSQKLLIHEFS 62

Query: 62  RKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQWL 121
           + +K+NN E+KTLLSIGGDDAKA+RFA MAA+  +RA+F+R+ I+KAREGNFDG+DLQWL
Sbjct: 63  KTVKQNNPELKTLLSIGGDDAKAERFAAMAASFFHRAAFVRTTIAKAREGNFDGLDLQWL 122

Query: 122 YPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRTL 181
           YPSSVEDM NFE L+IEC+N +++E  KS  P+LILVATVFYSP+++NI YP QV+ RTL
Sbjct: 123 YPSSVEDMANFETLIIECHNAIIRETTKSSLPKLILVATVFYSPHMENITYPTQVMIRTL 182

Query: 182 DWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPFH 241
           DW+N+IAYDI+TP SSS ETGPSSAF+NP+R  L G FG++ WI++GL SKMMVFG+PFH
Sbjct: 183 DWVNVIAYDIFTPASSSNETGPSSAFFNPRRRDLCGSFGIKIWIEVGLHSKMMVFGIPFH 242

Query: 242 GWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIGY 301
           GWAWKL + + H+VFS A GAA+GE+I MEGLIEYRN+KKFIA+ DANNV ++++F I Y
Sbjct: 243 GWAWKLNSSENHQVFSAAHGAAQGEDISMEGLIEYRNVKKFIAEKDANNVALDQRFAIAY 302

Query: 302 TYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREWKK 358
           TY  STWIAYEDE+TIK KIS++K D  L GYF  NIAADDEN SLA  AS +W+K
Sbjct: 303 TYSDSTWIAYEDEETIKLKISSVKMDLELLGYFASNIAADDENLSLANTASDQWRK 358

BLAST of Tan0018166 vs. ExPASy TrEMBL
Match: A0A0A0LL51 (Glyco_18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G008760 PE=4 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 8.8e-138
Identity = 248/369 (67.21%), Postives = 294/369 (79.67%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFC-GYVGLNSQTYELSISDSAKPLIDQ 60
           MA   +VN GYWFTHTN H PIS+IDTNLFTHLFC G V +N QTYELSIS+S KPLIDQ
Sbjct: 1   MAFKIFVNGGYWFTHTNTHFPISKIDTNLFTHLFCGGCVSINPQTYELSISNSTKPLIDQ 60

Query: 61  FSRKIKENNSEMKTLLSI--GGDDAKAD--RFATMAANSSNRASFIRSFISKAREGNFDG 120
           FS+ IKE N E+KTLLSI    DDAK D  RFA MA +SSNRA+FIRS ISKARE NFDG
Sbjct: 61  FSKAIKEKNHEVKTLLSINESEDDAKGDHGRFAAMARDSSNRATFIRSLISKARESNFDG 120

Query: 121 VDLQWLYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYI---KNIKY 180
           VDLQWLYPSS E+M NFEAL++ECYN  VKE ++ H+PRLILVATVFY PYI     + Y
Sbjct: 121 VDLQWLYPSSREEMANFEALILECYNDFVKEGKECHKPRLILVATVFYLPYIGKDDGVMY 180

Query: 181 PIQVIRRTLDWINIIAYDIYTPTSSSLETGPSSA-FYNPKRNSLSGYFGMRTWIQLGLPS 240
           PI+ +RRTLDW+N+IAYDI+TP SSS ETGPSSA F NP + +L GYFG+RTWI+ G+P+
Sbjct: 181 PIETMRRTLDWVNVIAYDIFTPISSSNETGPSSALFNNPSKATLGGYFGIRTWIEKGMPT 240

Query: 241 KMMVFGLPFHGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIAD-NDANN 300
           K M+FGLPFHGWAWKL+    H VFS AQG A+G+NI MEGLIEYRN+KKFI D N+  N
Sbjct: 241 KRMIFGLPFHGWAWKLERSYNHNVFSPAQGPAQGQNISMEGLIEYRNVKKFIVDNNNVTN 300

Query: 301 VLIERKFVIGYTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKA 360
           VLI+ K+ I YT+C +TWIAYE E+TI  KI+  K + ++ GYF  N+AADD++HSLAKA
Sbjct: 301 VLIDHKYPIAYTHCDNTWIAYESEETITAKIAKAKINLAMLGYFASNVAADDDHHSLAKA 360

BLAST of Tan0018166 vs. ExPASy TrEMBL
Match: A0A6J1CPE3 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111012956 PE=4 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 8.8e-106
Identity = 186/247 (75.30%), Postives = 217/247 (87.85%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQF 60
           MA   +VNAGYWFT TN+ LP S ID NL+ HLFC + GLNSQTYELSISDSAKPLI  F
Sbjct: 1   MACITFVNAGYWFTQTNSQLPASRIDANLYPHLFCAHAGLNSQTYELSISDSAKPLIHHF 60

Query: 61  SRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQW 120
           S+ +KENNSE+KTLLSIGGD+AKA+RF  MAANSS+RA+FIR+ ISKAREGNFDG+DLQW
Sbjct: 61  SQTVKENNSELKTLLSIGGDEAKAERFGAMAANSSHRAAFIRTSISKAREGNFDGLDLQW 120

Query: 121 LYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRT 180
           LYPSSVEDM NFEAL+IEC+  V++E++KS EPRLI VATVFYSP+++N+ YPIQV+ RT
Sbjct: 121 LYPSSVEDMANFEALIIECHRAVIRESRKSEEPRLIFVATVFYSPHLENLTYPIQVMIRT 180

Query: 181 LDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPF 240
           LDW+N+IAYDI+TPT SS E GPSSAF+NP+R S SG FG+RTWIQLGLPSKMMVFG+PF
Sbjct: 181 LDWVNVIAYDIFTPTLSSNEVGPSSAFFNPRRRSSSGDFGIRTWIQLGLPSKMMVFGVPF 240

Query: 241 HGWAWKL 248
           HGWAWKL
Sbjct: 241 HGWAWKL 247

BLAST of Tan0018166 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 283.9 bits (725), Expect = 1.9e-76
Identity = 151/350 (43.14%), Postives = 214/350 (61.14%), Query Frame = 0

Query: 7   VNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQFSRKIKE 66
           V A YWF    +  P+++ID++LFTHLFC +  LNSQT ++++S + +P    F++ ++ 
Sbjct: 28  VKASYWF--PASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 67  NNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQWLYPSSV 126
            N  +KTLLSIGG  A    +A+MA+N ++R SFI S I  AR   F G+DL W YPSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 127 EDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRTLDWINI 186
            +MTNF  L+ E  + VV EA  S +PRL+L A VFYS    ++ YP+  +  +LDW+N+
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYYSVLYPVSAVASSLDWVNL 207

Query: 187 IAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPFHGWAWK 246
           +AYD Y P  S + TGP +A ++P     SG  G R+WIQ GLP+K  V G P++G+AW+
Sbjct: 208 MAYDFYGPGWSRV-TGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVLGFPYYGYAWR 267

Query: 247 LKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIG-YTYCG 306
           L N   H  ++   GAA    I  +G I Y  I+KFI DN A  V      V+G Y Y G
Sbjct: 268 LTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGATTVY--NSTVVGDYCYAG 327

Query: 307 STWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAASREW 356
           + WI Y+D  +I  K+   K+   L GYF +++ ADD N  L++AAS+ W
Sbjct: 328 TNWIGYDDNQSIVTKVRYAKQR-GLLGYFSWHVGADD-NSGLSRAASQAW 366

BLAST of Tan0018166 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 253.1 bits (645), Expect = 3.6e-67
Identity = 138/346 (39.88%), Postives = 199/346 (57.51%), Query Frame = 0

Query: 7   VNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQFSRKIKE 66
           V A YWF  + +  P+++ID++LFTHLFC +  +N+ TY++ +S   KP    F++ ++ 
Sbjct: 26  VKATYWFAESES--PLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVRR 85

Query: 67  NNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQWLYPSSV 126
            N  +KTLLSIGGD      FA+MA+N ++R  FI S I  AR   F G+DL W YPS  
Sbjct: 86  RNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSIT 145

Query: 127 EDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRTLDWINI 186
            +M NF  L+ E    V  EA+ S +PRL+L A VFYS    ++ +P+  +  +LDW+N+
Sbjct: 146 TEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVFYSYSYYSVLHPVNAVADSLDWVNL 205

Query: 187 IAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPFHGWAWK 246
           +AYD Y   SS +   P +  Y+P     SG  G+R W Q GLP+K  V G P +G+AW 
Sbjct: 206 VAYDFYESGSSRVTCSP-APLYDPITTGPSGDAGVRAWTQAGLPAKKAVLGFPLYGYAWC 265

Query: 247 LKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIGYTYCGS 306
           L + K H  ++ + G A    I  +G I Y  I++FI DN A  ++     V  Y Y   
Sbjct: 266 LTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKA-TMVYNSNLVQNYCYAKK 325

Query: 307 TWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAAS 353
           TWI Y+D  +I  K+   K+   L GYF ++I ADD N  L++A S
Sbjct: 326 TWIGYDDNQSIVMKVKYAKQR-GLLGYFSWHIGADD-NSRLSRAGS 361

BLAST of Tan0018166 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 246.1 bits (627), Expect = 4.4e-65
Identity = 139/352 (39.49%), Postives = 207/352 (58.81%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQF 60
           M+ ++ V A YWF  T+   P ++ID++LFTHLFC +  L +++YE++I+   +     F
Sbjct: 1   MSESDVVKASYWFPATD--FPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAF 60

Query: 61  SRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQW 120
           +  +++ N  +KTLLSIGG +A  D FA+MA+N  +RASFI+S I+ AR   F G+DL W
Sbjct: 61  TETVQQRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDW 120

Query: 121 LYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRT 180
            YP + E+M +F  L+ E  + V  E+  S    LIL A V+YS   + + YP+  I  +
Sbjct: 121 EYPRNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAVYYSSNYQGVPYPVLAISNS 180

Query: 181 LDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNSLSGYFGMRTWIQLGLPSKMMVFGLPF 240
           LDWIN++AYD Y P  S++ TGP ++ Y P  +  SG  G+R W + GLP+K  V G P+
Sbjct: 181 LDWINLMAYDFYGPGWSTV-TGPPASLYLP-TDGRSGDSGVRDWTEAGLPAKKAVLGFPY 240

Query: 241 HGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVIG 300
           +GWAW L +   +   +   G A    I  +G I YR ++ +I DN A  V  +   V  
Sbjct: 241 YGWAWTLADPDVNGYDANTTGPA----ISDDGEISYRQLQTWIVDNGATKV-HDDMMVGD 300

Query: 301 YTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAAS 353
           Y Y G+TWI Y+ E +I  K+   K+   L GYF +++  DD N  L+ A S
Sbjct: 301 YCYAGTTWIGYDSEKSIVTKVIYAKQK-GLLGYFSWHVGGDD-NSELSSAGS 341

BLAST of Tan0018166 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 244.2 bits (622), Expect = 1.7e-64
Identity = 143/366 (39.07%), Postives = 211/366 (57.65%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNH--------LPISEIDTNLFTHLFCGYVGLNSQTYELSISDS 60
           +  N+ V A YWF    +          P S ID+ LFTHLFC +  ++S T+E++IS +
Sbjct: 8   LRTNSIVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAA 67

Query: 61  AKPLIDQFSRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGN 120
                  F+  +KE N++++TLLSIGG DA     A+MA+NS NR +FI S I  AR+ +
Sbjct: 68  NSYQFSSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKD 127

Query: 121 FDGVDLQWLYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKY 180
           F G+DL W YPS+  +MTNF  L+ E    VV+E+ K+++  L+L A V+YSP    ++Y
Sbjct: 128 FYGLDLAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQYDGVEY 187

Query: 181 PIQVIRRTLDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNS-LSGYFGMRTWI-QLGLP 240
           P++ I   LD++NI+AYD Y P  S +   P++ F++P   +  SG  G+R W+ +  LP
Sbjct: 188 PVKAIADNLDFVNIMAYDFYGPGWSPVTGPPAALFHDPSNPAGRSGNSGLRKWLDEAKLP 247

Query: 241 SKMMVFGLPFHGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANN 300
            K  V G P+ GWAW L++ + +   +   GAA    I  +G I Y  I+ +I DN A  
Sbjct: 248 PKKAVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIRNYIVDNGA-- 307

Query: 301 VLIERKFVIG-YTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAK 356
                  VIG Y Y G+TWI Y+D  +I +K+   K    L GYF +++ A D N  L++
Sbjct: 308 ATFHDPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFT-GLLGYFSWHVGA-DYNCGLSR 365

BLAST of Tan0018166 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 239.2 bits (609), Expect = 5.4e-63
Identity = 138/354 (38.98%), Postives = 204/354 (57.63%), Query Frame = 0

Query: 1   MANNNYVNAGYWFTHTNNHLPISEIDTNLFTHLFCGYVGLNSQTYELSISDSAKPLIDQF 60
           +  N+ V A YW     N  P   ID+  FTHLFC +  ++S T+E++IS +    +  F
Sbjct: 8   LRTNSIVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSF 67

Query: 61  SRKIKENNSEMKTLLSIGGDDAKADRFATMAANSSNRASFIRSFISKAREGNFDGVDLQW 120
           +  +K+ N++++TLLSIGG DA     A+MA+NS NR +FI S I  AR+ +F G+DL W
Sbjct: 68  THTVKDKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAW 127

Query: 121 LYPSSVEDMTNFEALVIECYNGVVKEAQKSHEPRLILVATVFYSPYIKNIKYPIQVIRRT 180
            YPS+  +M NF  LV E    VV+E+ ++++  L+L A V+YSP     +YP+Q I   
Sbjct: 128 EYPSNDVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDYYGEEYPVQAIADN 187

Query: 181 LDWINIIAYDIYTPTSSSLETGPSSAFYNPKRNS-LSGYFGMRTWIQLGLPSKMMVFGLP 240
           LD++NI+AYD Y P  S + TGP +A ++P   +  SG  G+  W++  LP+K  V G  
Sbjct: 188 LDFVNIMAYDFYGPGWSPV-TGPPAALFDPSNPAGRSGDSGLSKWLEAKLPAKKAVLGFS 247

Query: 241 FHGWAWKLKNWKKHEVFSEAQGAARGENICMEGLIEYRNIKKFIADNDANNVLIERKFVI 300
           + GWAW L++ + +   +   GAA    I  +G I Y  I+ +I DN A         VI
Sbjct: 248 YCGWAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDNGA--ATFHDPAVI 307

Query: 301 G-YTYCGSTWIAYEDEDTIKFKISTLKRDFSLNGYFVFNIAADDENHSLAKAAS 353
           G Y Y G+TWI Y+D  +I  K+   K    L GYF +++ A D N  L++A S
Sbjct: 308 GFYCYVGTTWIGYDDNQSIVSKVRYAKLK-GLLGYFSWHVGA-DYNCGLSRAGS 352

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818622.7e-7543.14Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1[more]
A0A1B1J8Z23.0e-5035.31Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2[more]
A0A072VEP03.1e-4733.43Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1[more]
A0A072UR654.9e-4531.14Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1[more]
U5N4E31.9e-4131.07Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023545460.16.2e-17079.78chitinase-3-like protein 2 [Cucurbita pepo subsp. pepo][more]
XP_022999353.11.7e-16778.96chitinase-3-like protein 2 [Cucurbita maxima][more]
KAG6599436.11.4e-16678.42Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7030351.13.2e-16678.42Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6599363.18.3e-15978.80Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1KJH18.1e-16878.96chitinase-3-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111493752 PE=4 SV=1[more]
A0A6J1CQ346.9e-15171.71chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013117 PE=4 SV=1[more]
A0A6J1CQ391.3e-14468.54oviduct-specific glycoprotein-like OS=Momordica charantia OX=3673 GN=LOC11101312... [more]
A0A0A0LL518.8e-13867.21Glyco_18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G008760 PE... [more]
A0A6J1CPE38.8e-10675.30chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111012956 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G19810.11.9e-7643.14Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19820.13.6e-6739.88Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19800.14.4e-6539.49Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19760.11.7e-6439.07Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19750.15.4e-6338.98Glycosyl hydrolase family protein with chitinase insertion domain [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011583Chitinase IISMARTSM006362g34coord: 6..341
e-value: 7.1E-53
score: 191.7
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 23..342
e-value: 2.5E-82
score: 278.9
NoneNo IPR availablePANTHERPTHR11177CHITINASEcoord: 4..356
NoneNo IPR availablePANTHERPTHR11177:SF351CHITINASE-LIKE PROTEIN-RELATEDcoord: 4..356
IPR029070Chitinase insertion domain superfamilyGENE3D3.10.50.10coord: 240..311
e-value: 2.5E-82
score: 278.9
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 9..341
e-value: 7.2E-57
score: 193.5
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 6..358
score: 45.438148
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 7..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0018166.1Tan0018166.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006032 chitin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0004568 chitinase activity
molecular_function GO:0008061 chitin binding