Tan0018013 (gene) Snake gourd v1

Overview


Name	Tan0018013
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	calumenin-B
Location	LG04: 3637167 .. 3640340 (+)
RNA-Seq Expression	Tan0018013
Synteny	Tan0018013

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CATACTCCCAAAGCACACTGTGAACACAAGTTTTCTTTTCGCCATTGTTGATCAAACTCGAAGAAGCTCTGCATTTTCTCAAACAAAAACCAATCTTCCACAATGGGGAGACTCTCGATTTTAATCTACATATCAATCTTTTCCCTTCTTCTTCTCCTCATCTATCACACCCCAACTCAACACCTCCGCCACCGCCGCCTCCGCCTCCGCTCCAATTTCACCTTCAATCCCTCTCTCCACCGCCAACAACACGACCACCATATCCCCTTCGACCCTCTCATCGCCGACATCCAGCTCCGCCGCGAGGTCGAGGAACGGCAGAAGCATTATTCCCTTCAACATTCCCACTTCGACGACTCTGCTTCGGCCGCCGAGTCCCATTCCGAGTGGGAAGAATTCATCAACGACGAGGACAGGTTCAATGTCACCGAAAGATTGGCCTCCCTTTTCCCCAAAATCGATGTCAATCCTCGTGATGGGTTTGTCTCTGCAGAGGAATTGACTCAGTGGAACTTGCGGCAGGCCGAGAATGATGCTCTGCATCGGACTGAAAGGGAATTTCAAGTTCACGATGAGGATCGTGATGGGTTTGTTTCCTTTTCTGAGTACGAGCCCCCCAGTTAGGTTATGACTGCCGGTAGATGTTCTTGATCTTTCTTGCTACTTTTTCAATGGGATTTTACTGTTGTTTGTTGGCCTTTGCAAATTAATGTGTTGTTGTTTGGTGAATTTATATTGTAGTATCTTTTGTCGATTACTTTGGCAATTATAGTGTACTATTTTATATGATATGTTTGTTTCCCAATGGCATTTATGATTTACTTTGCCAGTTTTTGAAGTAGAAAGTTACCTTTTGGCTAAGAGAAATATGGATATTGTTTGTGTATATGCTTCTGTGAGATATTATATATCAACACTCTTTGAGTTCTCTTATGTAGCTTCTAAAGGGAATATTTAGCCCATATAGTTTATGAAGTTTGGGTTGAGACATTTGAGTTTGTGTAGTATGGTACAAAGAAGTTAAAACTCGGGTAATTTCTTTTCCTTTTAAGTGTAGCATGTTTGATATGTGAGTTTATGATTTTTTTTAAAGTATAACAAGTGTTTGATTCATGGGTTTATGACTTTCTTCTGTTGGGAAAAGGTGCAATCCGTGTTTGATTTGTGAGTTCTTGACGTATAATCGTCTACCATATAATATAAAATGTAGGTTTCACAAGCTTAGATTTTTGTTATTTATATTTATGTTGACCCTAAGAATAACAATTCCTTAGTTTGGAATAAGCAAATCTATAAACTAAATATAGGCTTGGTTTCTAAACTTGTTACACAAGCCAAACGCCCTAAATGGCTTCTTCTTGTTCTCATAGAGGGTTTTAAAGATTCCAGCAGTGTTGTTTATGTTACATATTTTGATATTTAATTTCCAATGTGTTCATCAGATAATGGTTCTTTCGGCCATGATCTTGACTGGTGGAATGAGGAGCACTTCAATGCATCAGATGCAGATGAGGATGGGCTTTTGGACATAATTGAATTCAATGAGTAAGCTTCTAAAACTTCACCTAGTCTCTTTTTATCCCACCATCAATCATCATTTGAAAACGATTAGAAAAAGAAGTTTGTATTTCATCAATTTCTCTTACTCGATACTTAAGTGGTCCGTGATCTACTCGTTTTCAGCTTCTTGTATCCTGCTGACAGCAAGAATCCAAAGGTGCTTCTATGGCTGTGTGCAGATGTAGTAAGGTAGATTCCTTTATGCAGTTTCAAAAACCTTTGGTTCATACTTAAATTTGGCCTTCTGCAGTTTGTATTTGTACTTAACTGTTGCTGTAATTTCACAAAGAAGCTTATTACCAAGATGGTTAAACATAGTGAACAAAATACAGCAAGAAAGGCCTTAGAATTACCAGATTGTAATACTTTTTCCAATCTTCTCGGTTTCGAAACCATCTGTTGTGTTTGAAGTTAGAATTGATTCAGTCTCACATCATCATTCCGCTGACCACTTGCTTTATAATGTCTCCTCTTAGGGAAAAAGATGAAGACAAAGATGGAAAGCTCAACTTCAGTGAGTTTTTCCATGGAATCTTCGATTTAGTAAGAAGATACGATGAAGGTTACGATTTGTCGAATCAGGATGACGATGCTACGGAGGCACTGGCAACAAAGATCTTCACTGAGCTTGACAAAGACTTTGATGGGTAAAACATTTTCCCTACAGATACCTTTTTGCTGCAAATGATTCATTCTCTAAACAGTATATGTTGCTCTGCTTCTTTACAGGTACCTTTCAGCAACAGAACTGCTGCCTATAATTGGAAAAATCCATCCATCAGAGGCTTACTATGCAAAACAACAAGCAGAATACATCATCTCTCAGGTACTTTTGGTTGATAAAACTTGCCTGCTGCTTTTATTGTTGTTGTTTCATAAATATCTTACAGTCCAAAGGGGTTAAGCAATTCATAAAATCTTCTGTGATTGTGCCGTTTTTCGACTGAGAGCGAGTTTACTTCTATTTTTAGTAGTAAGAACCAATTCTGCTGGATTACTCTCAATTTGTTATTGGAACTCGAAATGTGGATAATGCATCATCAACCATATACATGTGTGAATGAGTTGATCTTTTACTAGATTGCATAGTTTGGGATGGATGGAAATTTCTACATAACAAAAATTGGTTTCTAGTAGTTGCTTATATGCAACGGGTTCTTAATTTACTTTCAACTAATCGGGGATCACTTACCAACCTAAGCATAGTTTAACTGATTAAAACATATATCTTCGACTAAGAGGTCGGAGGTTTGAATCTCTTCACCCTCACATTTTGTTGTACTAAAAAAATCCCGTCGGGATCAATGTAATTTAACATGGGGGAAATATGGCAAAAGGATCGAAAGGGTTATAAACTTGTATTAATCTTCACATACTCATTCTTAGGATCACGAGATGAACAACTTCGATCGTTTTGAAGGTCTTATTTCCTGTTCTTTTGTACACAAACACTGAAAAGCAGATTCTTGGTGTTGTTAATTGCAGGTAGATAGTGATGATGATGGACTTCTTACCTTGAAGGATATGATTGAGAACCCATCTGTGTTCTATAGCGCGGTTTATAGCGAAGATGAAATGGATTATTATCCAGATCACCACGACGAATTTCGTTAG

mRNA sequence

CATACTCCCAAAGCACACTGTGAACACAAGTTTTCTTTTCGCCATTGTTGATCAAACTCGAAGAAGCTCTGCATTTTCTCAAACAAAAACCAATCTTCCACAATGGGGAGACTCTCGATTTTAATCTACATATCAATCTTTTCCCTTCTTCTTCTCCTCATCTATCACACCCCAACTCAACACCTCCGCCACCGCCGCCTCCGCCTCCGCTCCAATTTCACCTTCAATCCCTCTCTCCACCGCCAACAACACGACCACCATATCCCCTTCGACCCTCTCATCGCCGACATCCAGCTCCGCCGCGAGGTCGAGGAACGGCAGAAGCATTATTCCCTTCAACATTCCCACTTCGACGACTCTGCTTCGGCCGCCGAGTCCCATTCCGAGTGGGAAGAATTCATCAACGACGAGGACAGGTTCAATGTCACCGAAAGATTGGCCTCCCTTTTCCCCAAAATCGATGTCAATCCTCGTGATGGGTTTGTCTCTGCAGAGGAATTGACTCAGTGGAACTTGCGGCAGGCCGAGAATGATGCTCTGCATCGGACTGAAAGGGAATTTCAAGTTCACGATGAGGATCGTGATGGGTTTGTTTCCTTTTCTGAGTACGAGCCCCCCAATAATGGTTCTTTCGGCCATGATCTTGACTGGTGGAATGAGGAGCACTTCAATGCATCAGATGCAGATGAGGATGGGCTTTTGGACATAATTGAATTCAATGACTTCTTGTATCCTGCTGACAGCAAGAATCCAAAGGTGCTTCTATGGCTGTGTGCAGATGTAGTAAGGGAAAAAGATGAAGACAAAGATGGAAAGCTCAACTTCAGTGAGTTTTTCCATGGAATCTTCGATTTAGTAAGAAGATACGATGAAGGTTACGATTTGTCGAATCAGGATGACGATGCTACGGAGGCACTGGCAACAAAGATCTTCACTGAGCTTGACAAAGACTTTGATGGGTACCTTTCAGCAACAGAACTGCTGCCTATAATTGGAAAAATCCATCCATCAGAGGCTTACTATGCAAAACAACAAGCAGAATACATCATCTCTCAGGTAGATAGTGATGATGATGGACTTCTTACCTTGAAGGATATGATTGAGAACCCATCTGTGTTCTATAGCGCGGTTTATAGCGAAGATGAAATGGATTATTATCCAGATCACCACGACGAATTTCGTTAG

Coding sequence (CDS)

ATGGGGAGACTCTCGATTTTAATCTACATATCAATCTTTTCCCTTCTTCTTCTCCTCATCTATCACACCCCAACTCAACACCTCCGCCACCGCCGCCTCCGCCTCCGCTCCAATTTCACCTTCAATCCCTCTCTCCACCGCCAACAACACGACCACCATATCCCCTTCGACCCTCTCATCGCCGACATCCAGCTCCGCCGCGAGGTCGAGGAACGGCAGAAGCATTATTCCCTTCAACATTCCCACTTCGACGACTCTGCTTCGGCCGCCGAGTCCCATTCCGAGTGGGAAGAATTCATCAACGACGAGGACAGGTTCAATGTCACCGAAAGATTGGCCTCCCTTTTCCCCAAAATCGATGTCAATCCTCGTGATGGGTTTGTCTCTGCAGAGGAATTGACTCAGTGGAACTTGCGGCAGGCCGAGAATGATGCTCTGCATCGGACTGAAAGGGAATTTCAAGTTCACGATGAGGATCGTGATGGGTTTGTTTCCTTTTCTGAGTACGAGCCCCCCAATAATGGTTCTTTCGGCCATGATCTTGACTGGTGGAATGAGGAGCACTTCAATGCATCAGATGCAGATGAGGATGGGCTTTTGGACATAATTGAATTCAATGACTTCTTGTATCCTGCTGACAGCAAGAATCCAAAGGTGCTTCTATGGCTGTGTGCAGATGTAGTAAGGGAAAAAGATGAAGACAAAGATGGAAAGCTCAACTTCAGTGAGTTTTTCCATGGAATCTTCGATTTAGTAAGAAGATACGATGAAGGTTACGATTTGTCGAATCAGGATGACGATGCTACGGAGGCACTGGCAACAAAGATCTTCACTGAGCTTGACAAAGACTTTGATGGGTACCTTTCAGCAACAGAACTGCTGCCTATAATTGGAAAAATCCATCCATCAGAGGCTTACTATGCAAAACAACAAGCAGAATACATCATCTCTCAGGTAGATAGTGATGATGATGGACTTCTTACCTTGAAGGATATGATTGAGAACCCATCTGTGTTCTATAGCGCGGTTTATAGCGAAGATGAAATGGATTATTATCCAGATCACCACGACGAATTTCGTTAG

Protein sequence

MGRLSILIYISIFSLLLLLIYHTPTQHLRHRRLRLRSNFTFNPSLHRQQHDHHIPFDPLIADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPKIDVNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPPNNGSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDEDKDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELLPIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDYYPDHHDEFR

Homology

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BLAST of Tan0018013 vs. ExPASy Swiss-Prot
Match: Q66JA6 (45 kDa calcium-binding protein OS=Xenopus laevis OX=8355 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.0e-15
Identity = 80/292 (27.40%), Postives = 134/292 (45.89%), Query Frame = 0


Query: 95  EWEEFINDEDRFNVTERLASLFPKIDVNPRDGFVSAEELTQWNLRQAE---NDALHRTER 154
           E EEF  D +      +LA++F K+D N  D  +SA E+ +W + + E    +A++  + 
Sbjct: 84  ELEEFDEDSEPRRNRRKLAAIFAKVDRN-EDKQISASEMQRWIMEKTEEHFQEAVNENKL 143

Query: 155 EFQVHDEDRDGFVSFSEYEPPNNGSFGHD-----------------------LDWWNEEH 214
            F+  D D DG VS+ EY+     S G +                       LD   +  
Sbjct: 144 HFRAVDPDGDGHVSWDEYKIKFLASKGFNEKEVAEKLKNNEDLKIDEETQEVLDNLKDRW 203

Query: 215 FNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDEDKDGKLNFSEFFHGI 274
           F A +   D LL+  EF  FL+P  S+   +L ++  +++R+ D+D D KL  SEF    
Sbjct: 204 FQADNPPPDQLLNEEEFLSFLHPEHSRG--MLKFMVKEIIRDLDQDGDKKLTLSEFISLP 263

Query: 275 FDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELLPIIGKIHPSEAYYA 334
              V    E     + DDD       +    +D + DG ++  EL      + P   Y A
Sbjct: 264 VGTV----ENQQAQDIDDDWVRDRKKEYEEVIDANHDGIVTMEELEEY---MDPMNEYNA 323

Query: 335 KQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDE-MDYYPDHHDEF 360
             +A+ +I+  D + D LL+L+++++     YS  ++  + MDY  + H+EF
Sbjct: 324 LNEAKQMIAVADENQDHLLSLEEILK-----YSEYFTGSKLMDYARNVHEEF 360

[Close]

BLAST of Tan0018013 vs. ExPASy Swiss-Prot
Match: Q5BKL9 (45 kDa calcium-binding protein OS=Xenopus tropicalis OX=8364 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.1e-14
Identity = 79/292 (27.05%), Postives = 133/292 (45.55%), Query Frame = 0


Query: 95  EWEEFINDEDRFNVTERLASLFPKIDVNPRDGFVSAEELTQWNLRQAE---NDALHRTER 154
           E EEF  D +      +LA++F K+D N  D  +SA E+ +W + + E    +A++  + 
Sbjct: 84  EIEEFDEDSEPRRNRRKLAAIFAKVDRN-EDKQISANEMQRWIMEKTEEHFQEAVNENKL 143

Query: 155 EFQVHDEDRDGFVSFSEYEPPNNGSFGHD-----------------------LDWWNEEH 214
            F+  D D DG VS+ EY+     S G +                       LD   +  
Sbjct: 144 HFRAVDPDGDGHVSWDEYKIKFLASKGFNEKEVAEKLKNNEDLKIDEETQEVLDNLKDRW 203

Query: 215 FNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDEDKDGKLNFSEFFHGI 274
           F A +   D LL+  EF  FL+P  S+   +L ++  +++R+ D+D D KL  SEF    
Sbjct: 204 FQADNPPADQLLNEEEFLSFLHPEHSQG--MLKFMVKEIIRDLDQDGDKKLTLSEFISLP 263

Query: 275 FDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELLPIIGKIHPSEAYYA 334
              V    E     + DDD       +    +D + DG ++  EL      + P   Y A
Sbjct: 264 VGTV----ENQQAQDIDDDWVRDRKKEYEEVIDANHDGIVTMEELEEY---MDPMNEYNA 323

Query: 335 KQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDE-MDYYPDHHDEF 360
             +A+ +I+  D + D  L+L+++++     YS  ++  + MDY  + H+EF
Sbjct: 324 LNEAKQMIAVADENQDHHLSLEEILK-----YSEYFTGSKLMDYARNVHEEF 360

[Close]

BLAST of Tan0018013 vs. ExPASy Swiss-Prot
Match: Q7ZUC2 (45 kDa calcium-binding protein OS=Danio rerio OX=7955 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.3e-13
Identity = 76/292 (26.03%), Postives = 133/292 (45.55%), Query Frame = 0


Query: 95  EWEEFINDEDRFNVTERLASLFPKIDVNPRDGFVSAEELTQWNLRQAE---NDALHRTER 154
           E EEF  D +     ++L  +F K+D+N +D  VSA+E+ +W + + E    +A+   + 
Sbjct: 80  EMEEFEEDSEPRRNRKKLIEIFTKVDIN-KDRSVSAKEMQRWIMEKTEEHFQEAVRENKL 139

Query: 155 EFQVHDEDRDGFVSFSEY-----------------EPPNNGSFGHD------LDWWNEEH 214
            F+  D D DG V++ EY                 +  NN     D      L+   +  
Sbjct: 140 SFRAVDPDGDGLVTWDEYRVKFLASKGLNEKEVAEKIKNNEELKVDEETQEVLESLKDRW 199

Query: 215 FNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDEDKDGKLNFSEFFHGI 274
           F A +   D LL+  EF  FL+P  S+   +L ++  ++VR+ D+D DGKL  +EF    
Sbjct: 200 FQADNPPADQLLNEEEFLSFLHPEHSRG--MLRYMVKEIVRDLDQDGDGKLTLAEF---- 259

Query: 275 FDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELLPIIGKIHPSEAYYA 334
             L     E     + DDD       +    +D + D  ++  EL      + P   + A
Sbjct: 260 ISLPMGTVENQQAQDIDDDWVRERKKEFEEVIDANHDTIVTMEELEEY---MDPMNEHNA 319

Query: 335 KQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDE-MDYYPDHHDEF 360
             +A+ +I+  D + +  L L+++++     YS  ++  + MDY  + H+EF
Sbjct: 320 LNEAKQMIAVADENQNHNLELEEILK-----YSEYFTGSKLMDYARNVHEEF 356

[Close]

BLAST of Tan0018013 vs. ExPASy Swiss-Prot
Match: Q5ZKE5 (45 kDa calcium-binding protein OS=Gallus gallus OX=9031 GN=SDF4 PE=2 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 6.2e-13
Identity = 75/292 (25.68%), Postives = 133/292 (45.55%), Query Frame = 0


Query: 95  EWEEFINDEDRFNVTERLASLFPKIDVNPRDGFVSAEELTQWNLRQAE---NDALHRTER 154
           E EEF  D +     ++L  +F K+D++  D  +SA+E+ +W + + +    +A+   + 
Sbjct: 80  EMEEFEEDSEPRKNRKKLMVIFSKVDID-NDKKISAKEMQRWIMEKTDEHFQEAVEENKM 139

Query: 155 EFQVHDEDRDGFVSFSEY-----------------EPPNNGSFGHD------LDWWNEEH 214
            F+  D D DG VS+ EY                 +  NN     D      LD   +  
Sbjct: 140 HFRAVDPDGDGHVSWDEYKIKFLASKGLNEKEIAEKIKNNEELKIDEETQEVLDNLKDRW 199

Query: 215 FNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDEDKDGKLNFSEFFHGI 274
           + A +   D LL+  EF  FL+P  S+   +L ++  +++R+ D+D D KL  SEF    
Sbjct: 200 YQADNPPPDMLLNEEEFLSFLHPEHSRG--MLKFMVKEIIRDLDQDGDKKLTLSEFISLP 259

Query: 275 FDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELLPIIGKIHPSEAYYA 334
              V    E     + DDD  +    +    +D + DG ++  EL      + P   Y A
Sbjct: 260 VGTV----ENQQAQDIDDDWVKDRRKEFEDVIDANHDGIVTMEELEEY---MDPMNEYNA 319

Query: 335 KQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDE-MDYYPDHHDEF 360
             +A+ +I+  D + +  L L+++++     YS  ++  + MDY  + H+EF
Sbjct: 320 LNEAKQMIAVADENQNHHLELEEILK-----YSEYFTGSKLMDYARNVHEEF 356

[Close]

BLAST of Tan0018013 vs. ExPASy Swiss-Prot
Match: B5X4E0 (Calumenin-B OS=Salmo salar OX=8030 GN=calub PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 6.2e-13
Identity = 81/312 (25.96%), Postives = 138/312 (44.23%), Query Frame = 0


Query: 82  HFDDSASAAESHSEWEEFINDEDRF---------------NVTERLASLFPKIDVNPRDG 141
           H DD  S+ + H + E F  D + F                  ERL  L  +ID   +DG
Sbjct: 30  HHDDPLSSRD-HEDAENFDYDHEAFLGQDEAKTFDQLTPEESKERLGMLVERID-EDKDG 89

Query: 142 FVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPPNNGSFGHDLD---- 201
           +VS EE+ +W     +       +R+++ HD + DG VS+ EY+   N ++G+ LD    
Sbjct: 90  YVSVEEMKKWIKHSQKRWIYDDVDRQWKGHDHNGDGLVSWEEYK---NATYGYILDDPDP 149

Query: 202 ----------WWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKD 261
                       +E  F  SD D D   +  EF  FL+P +  + K ++ L  + + + D
Sbjct: 150 EDGFSYRQMISRDERRFKMSDLDADLKANKEEFTAFLHPEEYDHMKDIVVL--ETMEDID 209

Query: 262 EDKDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATE----ALATKIFTEL-DKDFDGY 321
           ++ DG ++  E+               D+ NQ+ D +E        + FTE  D + DG 
Sbjct: 210 KNGDGFIDLEEYIG-------------DMYNQEGDPSEPEWVRTEREQFTEFRDTNKDGR 269

Query: 322 LSATELLPIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSED 360
           +   E       I PS+  +A+ +A++++ + D+D DG LT  +++E   +F  +     
Sbjct: 270 MDKEETK---DWILPSDYDHAEAEAKHLVYESDNDKDGKLTKAEIVEKYDLFVGS--QAT 316

[Close]

BLAST of Tan0018013 vs. NCBI nr
Match: XP_038892509.1 (calumenin-B-like [Benincasa hispida] >XP_038892510.1 calumenin-B-like [Benincasa hispida])

HSP 1 Score: 548.1 bits (1411), Expect = 5.6e-152
Identity = 284/368 (77.17%), Postives = 315/368 (85.60%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQ--HLRHRRLRLRSNFTFNPSLHRQQHDHHIPFDP 60
           MG+L+ILIYISIFSLLL+LI HTPT+   LRHRRLRLRSNFTFNP LHRQQHDH+IPFDP
Sbjct: 1   MGKLAILIYISIFSLLLILISHTPTRRLRLRHRRLRLRSNFTFNPLLHRQQHDHYIPFDP 60

Query: 61  LIADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPK 120
           LIADIQLRRE +E QKH SLQHS      +AA+SH EWEEFINDEDRFNVTERL  LFPK
Sbjct: 61  LIADIQLRREDDEWQKH-SLQHS------AAADSHPEWEEFINDEDRFNVTERLFWLFPK 120

Query: 121 IDVNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPP------ 180
           IDV+P DGFVSAEELT+WNL+QA N+ALHRTE+EFQ HD++RDGFVSF+EYEPP      
Sbjct: 121 IDVDPSDGFVSAEELTRWNLQQAMNEALHRTEKEFQSHDDNRDGFVSFAEYEPPTWVLAA 180

Query: 181 NNGSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKD 240
            N SFG+D+ WWNEEHFNASDAD DG+LDI EFNDFL+PADSK+PK+LLWLCADVVRE+D
Sbjct: 181 GNDSFGYDIGWWNEEHFNASDADGDGVLDINEFNDFLHPADSKSPKLLLWLCADVVRERD 240

Query: 241 EDKDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATE 300
            DKDGKLNFSEFF  IFDLVRR DE Y   N DDD  EA A K+F+ LDK+ DGYLS TE
Sbjct: 241 NDKDGKLNFSEFFPRIFDLVRRVDEDYSSLNWDDDELEARAKKMFSNLDKNSDGYLSTTE 300

Query: 301 LLPIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDYY 360
           LLPIIGKIHP EAYYAKQQAEYIIS  DSD+DGLLTL DMI+NP VFYS+V+SEDEMDYY
Sbjct: 301 LLPIIGKIHPPEAYYAKQQAEYIISLADSDNDGLLTLNDMIDNPFVFYSSVFSEDEMDYY 360

[Close]

BLAST of Tan0018013 vs. NCBI nr
Match: XP_008445899.1 (PREDICTED: calumenin-B [Cucumis melo] >KAA0034085.1 calumenin-B [Cucumis melo var. makuwa])

HSP 1 Score: 538.5 bits (1386), Expect = 4.4e-149
Identity = 276/366 (75.41%), Postives = 312/366 (85.25%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQHLRHRRLRLRSNFTFNPSLHRQQHDHHIPFDPLI 60
           MG+ S+LIYISIFSLLLLLI  TPT   RHRRLRLRSNFTFNP LHRQQHDH+I FDPLI
Sbjct: 1   MGKFSVLIYISIFSLLLLLISQTPTTVPRHRRLRLRSNFTFNPPLHRQQHDHYISFDPLI 60

Query: 61  ADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPKID 120
           +DI+LRRE +E QK  SL      ++ +A +SH EWEEFINDEDRFNVTERL  +FPKID
Sbjct: 61  SDIELRREDDEWQKQ-SL------ENLAAHDSHPEWEEFINDEDRFNVTERLFFIFPKID 120

Query: 121 VNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPPN------N 180
           V+P DGFVSAEELT+WNL+QA N+ALHRTEREFQ HDE+RDGFVSF+EYEPP+      N
Sbjct: 121 VDPSDGFVSAEELTRWNLQQAMNEALHRTEREFQSHDENRDGFVSFAEYEPPSWVLAAGN 180

Query: 181 GSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDED 240
            SFG+D+ WWNEEHFNASDAD DG+LD+ EFNDFL+PADSK+PK+LLWLCADVVRE+D D
Sbjct: 181 SSFGYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSPKLLLWLCADVVRERDND 240

Query: 241 KDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELL 300
           KDGKLNFSEFF  I DL+RR DE Y+ SN +D+  EALA K+F ELDKD DG+LS +ELL
Sbjct: 241 KDGKLNFSEFFPKILDLIRRVDEDYNSSNWEDEEPEALARKMFLELDKDSDGFLSTSELL 300

Query: 301 PIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDYYPD 360
           PIIGKIHPSEAYYAKQQAEYIISQ DSDDDGLLTL DMIENP VFYS+V+SEDEMDYYP 
Sbjct: 301 PIIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPFVFYSSVFSEDEMDYYP- 358

[Close]

BLAST of Tan0018013 vs. NCBI nr
Match: XP_011655504.1 (calumenin-B [Cucumis sativus] >XP_031741815.1 calumenin-B [Cucumis sativus] >KGN51567.1 hypothetical protein Csa_009120 [Cucumis sativus])

HSP 1 Score: 529.3 bits (1362), Expect = 2.7e-146
Identity = 271/366 (74.04%), Postives = 310/366 (84.70%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQHLRHRRLRLRSNFTFNPSLHRQQHDHHIPFDPLI 60
           MG+ S+LIYISIFSLLL+LI  TPT   RHRRLRLRSNFTFNP L R QHDH+I FDPLI
Sbjct: 1   MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI 60

Query: 61  ADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPKID 120
           +DI+LRRE +E  K  SL+      + +A +SH EWEEFINDEDRFNVTERL  +FPKID
Sbjct: 61  SDIELRREDDEWHKQ-SLK------NLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKID 120

Query: 121 VNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPPN------N 180
           V+P DGFVSAEELT+WNL+QA N+AL+RTEREFQ HD++RDGFVSF+EYEPP+      N
Sbjct: 121 VDPSDGFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGN 180

Query: 181 GSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDED 240
           GSFG+D+ WWNEEHFNASDAD DG+LD+ EFNDFL+PADSK+ K+LLWLCADVVRE+D D
Sbjct: 181 GSFGYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDND 240

Query: 241 KDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELL 300
           KDGKLNFSEFF  + DLVRR DE Y+ SN +D+  EALA K+F ELDKD DGYLS TE+L
Sbjct: 241 KDGKLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKKMFLELDKDSDGYLSTTEML 300

Query: 301 PIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDYYPD 360
           PIIGKIHPSEAYYAKQQAEYIISQ DSDDDGLLTL DMIENPSVFYS+V++EDEMDYYP 
Sbjct: 301 PIIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYP- 358

[Close]

BLAST of Tan0018013 vs. NCBI nr
Match: XP_022999248.1 (reticulocalbin-2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 518.5 bits (1334), Expect = 4.7e-143
Identity = 270/368 (73.37%), Postives = 303/368 (82.34%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQHL--RHRRLRLRSNFTFNPSLHRQQHDHHIPFDP 60
           MG+LSI IYISIFSLLL+L +HTP++ +  RHRRLRLRSNFTFN  LHRQQ DH+ PFDP
Sbjct: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60

Query: 61  LIADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPK 120
           LIA+IQLRRE EE Q H SL  S  D    AA+SH EWEEFINDEDRFNVTERL+SLFP 
Sbjct: 61  LIANIQLRREDEEWQNH-SLGPSQSD----AADSHPEWEEFINDEDRFNVTERLSSLFPN 120

Query: 121 IDVNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPPN----- 180
           IDVNP DGF+S EELT+WN +QA N ALHRTEREF+ HDEDRDGFVSF+EYEPP+     
Sbjct: 121 IDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAA 180

Query: 181 -NGSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKD 240
            N S G+D+ WWNEEHFNASDAD +G LD+ EFNDFL+PADS+NPK+LLWLC DVVRE+D
Sbjct: 181 GNSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERD 240

Query: 241 EDKDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATE 300
             KDGKL+FSEF   IFDLVRR DE Y   + DDD  EA A K+F ELDKD DGYLSATE
Sbjct: 241 NSKDGKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATE 300

Query: 301 LLPIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDYY 360
           LLP+IGKIHPSEAYYAKQQAEYIISQ DSD+D LLTL DMI+NP VFYS+V+SE EMDYY
Sbjct: 301 LLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYY 360

[Close]

BLAST of Tan0018013 vs. NCBI nr
Match: XP_022999241.1 (reticulocalbin-2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 518.1 bits (1333), Expect = 6.2e-143
Identity = 270/369 (73.17%), Postives = 303/369 (82.11%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQHL--RHRRLRLRSNFTFNPSLHRQQHDHHIPFDP 60
           MG+LSI IYISIFSLLL+L +HTP++ +  RHRRLRLRSNFTFN  LHRQQ DH+ PFDP
Sbjct: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60

Query: 61  LIADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPK 120
           LIA+IQLRRE EE Q H SL  S  D    AA+SH EWEEFINDEDRFNVTERL+SLFP 
Sbjct: 61  LIANIQLRREDEEWQNH-SLGPSQSD----AADSHPEWEEFINDEDRFNVTERLSSLFPN 120

Query: 121 IDVNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPP------ 180
           IDVNP DGF+S EELT+WN +QA N ALHRTEREF+ HDEDRDGFVSF+EYEPP      
Sbjct: 121 IDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAA 180

Query: 181 -NNGSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREK 240
            +N S G+D+ WWNEEHFNASDAD +G LD+ EFNDFL+PADS+NPK+LLWLC DVVRE+
Sbjct: 181 GSNSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRER 240

Query: 241 DEDKDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSAT 300
           D  KDGKL+FSEF   IFDLVRR DE Y   + DDD  EA A K+F ELDKD DGYLSAT
Sbjct: 241 DNSKDGKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSAT 300

Query: 301 ELLPIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDY 360
           ELLP+IGKIHPSEAYYAKQQAEYIISQ DSD+D LLTL DMI+NP VFYS+V+SE EMDY
Sbjct: 301 ELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDY 360

[Close]

BLAST of Tan0018013 vs. ExPASy TrEMBL
Match: A0A5A7SY06 (Calumenin-B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00730 PE=4 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.1e-149
Identity = 276/366 (75.41%), Postives = 312/366 (85.25%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQHLRHRRLRLRSNFTFNPSLHRQQHDHHIPFDPLI 60
           MG+ S+LIYISIFSLLLLLI  TPT   RHRRLRLRSNFTFNP LHRQQHDH+I FDPLI
Sbjct: 1   MGKFSVLIYISIFSLLLLLISQTPTTVPRHRRLRLRSNFTFNPPLHRQQHDHYISFDPLI 60

Query: 61  ADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPKID 120
           +DI+LRRE +E QK  SL      ++ +A +SH EWEEFINDEDRFNVTERL  +FPKID
Sbjct: 61  SDIELRREDDEWQKQ-SL------ENLAAHDSHPEWEEFINDEDRFNVTERLFFIFPKID 120

Query: 121 VNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPPN------N 180
           V+P DGFVSAEELT+WNL+QA N+ALHRTEREFQ HDE+RDGFVSF+EYEPP+      N
Sbjct: 121 VDPSDGFVSAEELTRWNLQQAMNEALHRTEREFQSHDENRDGFVSFAEYEPPSWVLAAGN 180

Query: 181 GSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDED 240
            SFG+D+ WWNEEHFNASDAD DG+LD+ EFNDFL+PADSK+PK+LLWLCADVVRE+D D
Sbjct: 181 SSFGYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSPKLLLWLCADVVRERDND 240

Query: 241 KDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELL 300
           KDGKLNFSEFF  I DL+RR DE Y+ SN +D+  EALA K+F ELDKD DG+LS +ELL
Sbjct: 241 KDGKLNFSEFFPKILDLIRRVDEDYNSSNWEDEEPEALARKMFLELDKDSDGFLSTSELL 300

Query: 301 PIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDYYPD 360
           PIIGKIHPSEAYYAKQQAEYIISQ DSDDDGLLTL DMIENP VFYS+V+SEDEMDYYP 
Sbjct: 301 PIIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPFVFYSSVFSEDEMDYYP- 358

[Close]

BLAST of Tan0018013 vs. ExPASy TrEMBL
Match: A0A1S3BDS4 (calumenin-B OS=Cucumis melo OX=3656 GN=LOC103488782 PE=4 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.1e-149
Identity = 276/366 (75.41%), Postives = 312/366 (85.25%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQHLRHRRLRLRSNFTFNPSLHRQQHDHHIPFDPLI 60
           MG+ S+LIYISIFSLLLLLI  TPT   RHRRLRLRSNFTFNP LHRQQHDH+I FDPLI
Sbjct: 1   MGKFSVLIYISIFSLLLLLISQTPTTVPRHRRLRLRSNFTFNPPLHRQQHDHYISFDPLI 60

Query: 61  ADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPKID 120
           +DI+LRRE +E QK  SL      ++ +A +SH EWEEFINDEDRFNVTERL  +FPKID
Sbjct: 61  SDIELRREDDEWQKQ-SL------ENLAAHDSHPEWEEFINDEDRFNVTERLFFIFPKID 120

Query: 121 VNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPPN------N 180
           V+P DGFVSAEELT+WNL+QA N+ALHRTEREFQ HDE+RDGFVSF+EYEPP+      N
Sbjct: 121 VDPSDGFVSAEELTRWNLQQAMNEALHRTEREFQSHDENRDGFVSFAEYEPPSWVLAAGN 180

Query: 181 GSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDED 240
            SFG+D+ WWNEEHFNASDAD DG+LD+ EFNDFL+PADSK+PK+LLWLCADVVRE+D D
Sbjct: 181 SSFGYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSPKLLLWLCADVVRERDND 240

Query: 241 KDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELL 300
           KDGKLNFSEFF  I DL+RR DE Y+ SN +D+  EALA K+F ELDKD DG+LS +ELL
Sbjct: 241 KDGKLNFSEFFPKILDLIRRVDEDYNSSNWEDEEPEALARKMFLELDKDSDGFLSTSELL 300

Query: 301 PIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDYYPD 360
           PIIGKIHPSEAYYAKQQAEYIISQ DSDDDGLLTL DMIENP VFYS+V+SEDEMDYYP 
Sbjct: 301 PIIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPFVFYSSVFSEDEMDYYP- 358

[Close]

BLAST of Tan0018013 vs. ExPASy TrEMBL
Match: A0A0A0KT37 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G579050 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.3e-146
Identity = 271/366 (74.04%), Postives = 310/366 (84.70%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQHLRHRRLRLRSNFTFNPSLHRQQHDHHIPFDPLI 60
           MG+ S+LIYISIFSLLL+LI  TPT   RHRRLRLRSNFTFNP L R QHDH+I FDPLI
Sbjct: 1   MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI 60

Query: 61  ADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPKID 120
           +DI+LRRE +E  K  SL+      + +A +SH EWEEFINDEDRFNVTERL  +FPKID
Sbjct: 61  SDIELRREDDEWHKQ-SLK------NLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKID 120

Query: 121 VNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPPN------N 180
           V+P DGFVSAEELT+WNL+QA N+AL+RTEREFQ HD++RDGFVSF+EYEPP+      N
Sbjct: 121 VDPSDGFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGN 180

Query: 181 GSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDED 240
           GSFG+D+ WWNEEHFNASDAD DG+LD+ EFNDFL+PADSK+ K+LLWLCADVVRE+D D
Sbjct: 181 GSFGYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDND 240

Query: 241 KDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELL 300
           KDGKLNFSEFF  + DLVRR DE Y+ SN +D+  EALA K+F ELDKD DGYLS TE+L
Sbjct: 241 KDGKLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKKMFLELDKDSDGYLSTTEML 300

Query: 301 PIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDYYPD 360
           PIIGKIHPSEAYYAKQQAEYIISQ DSDDDGLLTL DMIENPSVFYS+V++EDEMDYYP 
Sbjct: 301 PIIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYP- 358

[Close]

BLAST of Tan0018013 vs. ExPASy TrEMBL
Match: A0A6J1KJ28 (reticulocalbin-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.3e-143
Identity = 270/368 (73.37%), Postives = 303/368 (82.34%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQHL--RHRRLRLRSNFTFNPSLHRQQHDHHIPFDP 60
           MG+LSI IYISIFSLLL+L +HTP++ +  RHRRLRLRSNFTFN  LHRQQ DH+ PFDP
Sbjct: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60

Query: 61  LIADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPK 120
           LIA+IQLRRE EE Q H SL  S  D    AA+SH EWEEFINDEDRFNVTERL+SLFP 
Sbjct: 61  LIANIQLRREDEEWQNH-SLGPSQSD----AADSHPEWEEFINDEDRFNVTERLSSLFPN 120

Query: 121 IDVNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPPN----- 180
           IDVNP DGF+S EELT+WN +QA N ALHRTEREF+ HDEDRDGFVSF+EYEPP+     
Sbjct: 121 IDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAA 180

Query: 181 -NGSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKD 240
            N S G+D+ WWNEEHFNASDAD +G LD+ EFNDFL+PADS+NPK+LLWLC DVVRE+D
Sbjct: 181 GNSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERD 240

Query: 241 EDKDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATE 300
             KDGKL+FSEF   IFDLVRR DE Y   + DDD  EA A K+F ELDKD DGYLSATE
Sbjct: 241 NSKDGKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATE 300

Query: 301 LLPIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDYY 360
           LLP+IGKIHPSEAYYAKQQAEYIISQ DSD+D LLTL DMI+NP VFYS+V+SE EMDYY
Sbjct: 301 LLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYY 360

[Close]

BLAST of Tan0018013 vs. ExPASy TrEMBL
Match: A0A6J1KCI7 (reticulocalbin-2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 3.0e-143
Identity = 270/369 (73.17%), Postives = 303/369 (82.11%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQHL--RHRRLRLRSNFTFNPSLHRQQHDHHIPFDP 60
           MG+LSI IYISIFSLLL+L +HTP++ +  RHRRLRLRSNFTFN  LHRQQ DH+ PFDP
Sbjct: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60

Query: 61  LIADIQLRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLASLFPK 120
           LIA+IQLRRE EE Q H SL  S  D    AA+SH EWEEFINDEDRFNVTERL+SLFP 
Sbjct: 61  LIANIQLRREDEEWQNH-SLGPSQSD----AADSHPEWEEFINDEDRFNVTERLSSLFPN 120

Query: 121 IDVNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPP------ 180
           IDVNP DGF+S EELT+WN +QA N ALHRTEREF+ HDEDRDGFVSF+EYEPP      
Sbjct: 121 IDVNPTDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAA 180

Query: 181 -NNGSFGHDLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREK 240
            +N S G+D+ WWNEEHFNASDAD +G LD+ EFNDFL+PADS+NPK+LLWLC DVVRE+
Sbjct: 181 GSNSSSGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRER 240

Query: 241 DEDKDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSAT 300
           D  KDGKL+FSEF   IFDLVRR DE Y   + DDD  EA A K+F ELDKD DGYLSAT
Sbjct: 241 DNSKDGKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSAT 300

Query: 301 ELLPIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSEDEMDY 360
           ELLP+IGKIHPSEAYYAKQQAEYIISQ DSD+D LLTL DMI+NP VFYS+V+SE EMDY
Sbjct: 301 ELLPVIGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDY 360

[Close]

BLAST of Tan0018013 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 390.2 bits (1001), Expect = 1.8e-108
Identity = 202/397 (50.88%), Postives = 277/397 (69.77%), Query Frame = 0


Query: 1   MGRLSILIYISIFSLLLLLIYHTPTQ----------HLRHRRLRLRSNFTFNPSLHRQQH 60
           M + S+++YI++  L+L L+ ++P +          H +H RL+LRS+F F P+ H    
Sbjct: 1   MSKASVILYITVGILVLFLVSYSPKKKGDHDHHHGGHNQHHRLKLRSSFNFKPTRH---- 60

Query: 61  DHHIPFDPLIADIQLRREVEERQKHYSLQHSHFD--------------DSASAAESHSEW 120
              +PFDPL+AD++ RRE +E ++ Y ++HSH +              + A   ES  EW
Sbjct: 61  -DPVPFDPLVADMERRREDKEWERQY-IEHSHPELVSHSQKETTGGGHEHAPGHESQPEW 120

Query: 121 EEF------INDEDRFNVTERLASLFPKIDVNPRDGFVSAEELTQWNLRQAENDALHRTE 180
           EEF      +NDE++FNVT+RL  LFPKIDV+P DGF++  ELT+W ++ +  + +HRT+
Sbjct: 121 EEFMDAEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQ 180

Query: 181 REFQVHDEDRDGFVSFSEYEPP------NNGSFGHDLDWWNEEHFNASDADEDGLLDIIE 240
           R+  VHD ++DGF+SFSEYEPP      +N SFG+D+ WW EEHFNASDA+ DGLL++ E
Sbjct: 181 RDLDVHDRNKDGFISFSEYEPPSWVRKSDNNSFGYDMGWWKEEHFNASDANGDGLLNLTE 240

Query: 241 FNDFLYPADSKNPKVLLWLCADVVREKDEDKDGKLNFSEFFHGIFDLVRRYDE-GYDLSN 300
           FNDFL+PAD+KNPK+LLWLC + VRE+D DKDGK++F EFFHG+FD VR Y+E  ++ ++
Sbjct: 241 FNDFLHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTH 300

Query: 301 QDDDATEALATKIFTELDKDFDGYLSATELLPIIGKIHPSEAYYAKQQAEYIISQVDSDD 360
              D  E  A ++F++LDK+ DGYLS  ELLPII KIHP+E YYAKQQA+YIISQ DSD 
Sbjct: 301 PYHDLPEGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDK 360

[Close]

BLAST of Tan0018013 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 186.8 bits (473), Expect = 3.0e-47
Identity = 111/313 (35.46%), Postives = 171/313 (54.63%), Query Frame = 0


Query: 56  FDPLIADIQ-LRREVEERQKHYSLQHSHFDDSASAAESHSEWEEFINDEDRFNVTERLAS 115
           FDPL+  I+ L  E E   K            A+  E    +EE+   E R N T R+  
Sbjct: 48  FDPLVTRIERLSHEKEAGTKTV---------EAAKEEKDDMFEEYFAQERRLNTTMRIKF 107

Query: 116 LFPKIDVNPRDGFVSAEELTQWNLRQAENDALHRTEREFQVHDEDRDGFVSFSEYEPP-- 175
           LFP +D +PRDGFVS +EL  W ++Q E++ ++RT +E ++ D+D+DG ++F EY P   
Sbjct: 108 LFPLLDASPRDGFVSLKELQTWMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYLPQFS 167

Query: 176 ----NNGSFGH-DLDWWNEEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADV 235
                    GH +  WW E+ F  SD D +G LDI EFN+FL+P DS+N     W+  + 
Sbjct: 168 KQDIEKNEKGHGEAGWWMEQ-FKNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWVLKER 227

Query: 236 VREKDEDKDGKLNFSEFFHGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGY 295
           +   D + DGKL + EF    +++ + + +      +++D        +F E+D+D D +
Sbjct: 228 MTGMDTNGDGKLEYKEFVKNAYEMYKEFAK----FEKEEDENVPTPQLLFAEMDRDKDRF 287

Query: 296 LSATELLPIIGKIHPSEAYYAKQQAEYIISQVDSDDDGLLTLKDMIENPSVFYSAVYSE- 355
           L A EL PI+  + P E  YAK  + ++  + D D DG L+L++M+ +  VFY AV+ E 
Sbjct: 288 LVADELRPILQYLQPGEMSYAKFYSTFLCHEADEDKDGKLSLEEMLHHEDVFYKAVHHED 344

Query: 356 -DEMDYYPDHHDE 359
            D+ DY+   HDE
Sbjct: 348 LDDEDYF--DHDE 344

[Close]

BLAST of Tan0018013 vs. TAIR 10
Match: AT4G14640.1 (calmodulin 8 )

HSP 1 Score: 48.1 bits (113), Expect = 1.7e-05
Identity = 40/149 (26.85%), Postives = 69/149 (46.31%), Query Frame = 0


Query: 186 EEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDEDKDGKLNFSEFF 245
           +E F   D D DG + + E    +   D    +  L    D++ E D D +G + F+EF 
Sbjct: 15  KEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQEL---HDIITEIDSDSNGTIEFAEFL 74

Query: 246 HGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELLPIIGKIHPSEA 305
           + +   +           Q+ DA E L  + F   DKD +GY+SA+EL  ++  I+  E 
Sbjct: 75  NLMAKKL-----------QESDAEEEL-KEAFKVFDKDQNGYISASELSHVM--INLGEK 134

Query: 306 YYAKQQAEYIISQVDSDDDGLLTLKDMIE 335
               ++ E +I + D D DG +   + ++
Sbjct: 135 -LTDEEVEQMIKEADLDGDGQVNYDEFVK 145

[Close]

BLAST of Tan0018013 vs. TAIR 10
Match: AT3G22930.1 (calmodulin-like 11 )

HSP 1 Score: 45.4 bits (106), Expect = 1.1e-04
Identity = 40/148 (27.03%), Postives = 68/148 (45.95%), Query Frame = 0


Query: 186 EEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDEDKDGKLNFSEFF 245
           +E F   D D DG +   E    +   D    +  L    D++ E D D +G + FSEF 
Sbjct: 37  KEAFCLFDKDGDGCITADELATVIRSLDQNPTEQEL---QDMITEIDSDGNGTIEFSEFL 96

Query: 246 HGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELLPIIGKIHPSEA 305
           + + + +           Q+ DA E L  + F   DKD +GY+SA+EL  ++  I+  E 
Sbjct: 97  NLMANQL-----------QETDADEEL-KEAFKVFDKDQNGYISASELRHVM--INLGEK 156

Query: 306 YYAKQQAEYIISQVDSDDDGLLTLKDMI 334
               ++ + +I + D D DG +   + +
Sbjct: 157 -LTDEEVDQMIKEADLDGDGQVNYDEFV 166

[Close]

BLAST of Tan0018013 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 44.3 bits (103), Expect = 2.4e-04
Identity = 35/149 (23.49%), Postives = 66/149 (44.30%), Query Frame = 0


Query: 186 EEHFNASDADEDGLLDIIEFNDFLYPADSKNPKVLLWLCADVVREKDEDKDGKLNFSEFF 245
           +E F+  D D DG +   E    +        +  L    D++ E D D +G ++F EF 
Sbjct: 14  KEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAEL---QDMINEVDADGNGTIDFPEFL 73

Query: 246 HGIFDLVRRYDEGYDLSNQDDDATEALATKIFTELDKDFDGYLSATELLPIIGKIHPSEA 305
           + +   ++           D D+ E L  + F   DKD +G++SA EL  ++  +     
Sbjct: 74  NLMAKKMK-----------DTDSEEEL-KEAFRVFDKDQNGFISAAELRHVMTNLGEK-- 133

Query: 306 YYAKQQAEYIISQVDSDDDGLLTLKDMIE 335
               ++ E +I + D D DG +  ++ ++
Sbjct: 134 -LTDEEVEEMIREADVDGDGQINYEEFVK 144

The following BLAST results are available for this feature:

BLAST of Tan0018013 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q66JA6	1.0e-15	27.40	45 kDa calcium-binding protein OS=Xenopus laevis OX=8355 GN=sdf4 PE=2 SV=1	[more]
Q5BKL9	1.1e-14	27.05	45 kDa calcium-binding protein OS=Xenopus tropicalis OX=8364 GN=sdf4 PE=2 SV=1	[more]
Q7ZUC2	1.3e-13	26.03	45 kDa calcium-binding protein OS=Danio rerio OX=7955 GN=sdf4 PE=2 SV=1	[more]
Q5ZKE5	6.2e-13	25.68	45 kDa calcium-binding protein OS=Gallus gallus OX=9031 GN=SDF4 PE=2 SV=2	[more]
B5X4E0	6.2e-13	25.96	Calumenin-B OS=Salmo salar OX=8030 GN=calub PE=2 SV=1	[more]

BLAST of Tan0018013 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892509.1	5.6e-152	77.17	calumenin-B-like [Benincasa hispida] >XP_038892510.1 calumenin-B-like [Benincasa...	[more]
XP_008445899.1	4.4e-149	75.41	PREDICTED: calumenin-B [Cucumis melo] >KAA0034085.1 calumenin-B [Cucumis melo va...	[more]
XP_011655504.1	2.7e-146	74.04	calumenin-B [Cucumis sativus] >XP_031741815.1 calumenin-B [Cucumis sativus] >KGN...	[more]
XP_022999248.1	4.7e-143	73.37	reticulocalbin-2-like isoform X2 [Cucurbita maxima]	[more]
XP_022999241.1	6.2e-143	73.17	reticulocalbin-2-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Tan0018013 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SY06	2.1e-149	75.41	Calumenin-B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00730 PE=...	[more]
A0A1S3BDS4	2.1e-149	75.41	calumenin-B OS=Cucumis melo OX=3656 GN=LOC103488782 PE=4 SV=1	[more]
A0A0A0KT37	1.3e-146	74.04	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G579050 PE=4 SV=1	[more]
A0A6J1KJ28	2.3e-143	73.37	reticulocalbin-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=...	[more]
A0A6J1KCI7	3.0e-143	73.17	reticulocalbin-2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=...	[more]

BLAST of Tan0018013 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08580.1	1.8e-108	50.88	Calcium-binding EF hand family protein	[more]
AT4G27790.1	3.0e-47	35.46	Calcium-binding EF hand family protein	[more]
AT4G14640.1	1.7e-05	26.85	calmodulin 8	[more]
AT3G22930.1	1.1e-04	27.03	calmodulin-like 11	[more]
AT1G66410.1	2.4e-04	23.49	calmodulin 4	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 223..251 e-value: 2.7 score: 14.3 coord: 272..300 e-value: 4.5 score: 12.5 coord: 148..181 e-value: 150.0 score: 0.2
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 153..169 e-value: 0.15 score: 11.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 271..333 e-value: 6.4E-8 score: 32.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 268..303 score: 10.105932
None	No IPR available	GENE3D	1.10.238.10		coord: 49..177 e-value: 2.5E-8 score: 36.0
None	No IPR available	GENE3D	1.10.238.10		coord: 178..249 e-value: 3.5E-6 score: 29.1
None	No IPR available	GENE3D	1.10.238.10		coord: 250..337 e-value: 2.5E-10 score: 42.4
None	No IPR available	PANTHER	PTHR10827	RETICULOCALBIN	coord: 6..360
None	No IPR available	PANTHER	PTHR10827:SF83	BNAC09G47400D PROTEIN	coord: 6..360
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 157..169
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 232..244
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 193..205
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 320..332
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 187..339
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 109..219

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0018013.1	Tan0018013.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding