Tan0017851 (gene) Snake gourd v1

Overview
NameTan0017851
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPectin lyase-like superfamily protein
LocationLG04: 12224654 .. 12230306 (+)
RNA-Seq ExpressionTan0017851
SyntenyTan0017851
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAATTCCATTTCAACCCCGACCACAAACTGGTAGAGCAGAGAGAGGAAAGAAGAAAATCGCCGTAGTGGGCACCGACCGCCTCATCGAGTGGCCCACAGCGTGCGACGTCGCTAACCAGCTTCGTAGATAGATTCTAATGGAGGTTTCCAGTTCCATCAACGTGCGACCCATTTTCTGTTTCTTCTTCAAACCCCTTCTTTATACAACTTTCCAGGTATTCAACTATATAAACTGCCCCATTATTTCATGCTCTGTTCCTTTTCCCTCTCCTAACACTATCAAGTGGATTTTATTTTATTCATTTATCTTTCTAGATTCTGTTCTTCTATCTCTTTTTAATTTTATTGGGGTTTCTCATAGGCCACTGATCCAGTAACCCTTTTGCTTGGTGGTTTTGATTTTTTATGTTTGATGTGATTTTTGGGGTGCCATTTGTTTTTTTATACTTTTGTGTATTTTATGGCTTTCATGAATCTGCATTTTGGTTCTTTTTGAGTCAAGTCGAGTGGTTAGAAGTTCTTCTTTAAACTGTTCTGCTATTGGGTTTGATTGTATCTACTCCAAGAAGTGGATATATGAGTTGATCTAATTGAATCTTAAATTTAAATTTTTACTTGGAATAGAAATTGTATCCAAGTTTAAAGAAGAATGAATTTGATTGAAAGTAGTACTAAATGTGAATTACATGAATTTGAAAGTAGTATTAAATGTGAATTACTTTTTCTATAAAATTTCATGGGAAAAGCATTAATTAAGTGCTCTTAACATATCTTAAATGTTTCGAATGAGTTTGAAAGGTCTTTTGGTGTTTTAAGTAAATATCAATCTTTTCTAAACATTTTCTTTTTGAATAAAACACTAAGGATCGAAATCACTCTTGTTATACTATACCTGTTTTTTAATTATTTTGGGCATAACAAAAGTCATTTTGACCATTTCGAAATCACTCTTCCAAACATGTCCTTTTAAATCTTTCCCTTAAAAGCATTTTAAGAATATAAATAATACTTTTCAATTAACTAAAACACTTTCTTACTCTTACGAAAGTAATGTGAGTTTTTCCTAACAACTGGTTTCTTTTACCATGCTTTTTACTGGTTGAGAACATTTTTTTAAAAAATGTATTCTTAAGAATTTTAGGAACCTAAACTACGTTAAATAGTTGGGGTCTTTTTCAAAAAGTGAAGTAATACTATATGTGCGTGTGTGTATATATATATCCAAAATTATAATTCAAAAGATGGACGGTCCATGTCAAAAGAAAAAAAAAAAACAGAATCTCTTGCAATTTTAAGAATCTTTTCCTAACTCAAAGGGATAAATTTTATTTAAAGCCAACAAGTGCCATTATCATTATTATCATTATCTTTTTTGTAAGATTGATTCATCTGTATTTTGGAAGACTTACTCTTGAAAGCATTATCCATTCGCTTTGTGCCGTGGTAGCTTTCATAATTTGGCTGATTCATAATTTTATACACATCATACAAACAAATAAAGGTGCTACAAATAATTACAACTTTCACTAATTATAATACGATTCACTTGCTTAAGATGGAGTTTATTTTATGTTTATTCTTTTTGAGCTGGCTGCTGAGTTGACTGCCATTTGATGCCAATAGGTAATTTCTTTTGGGAAAATGGGATACTTTTTTTGAATATGACAGGCCTTTAATTCTCTATATAGTCTGAAGACTTCACTACCCTGTGGAATAGAACTTGGCTTAAATTAAGTCTCTAGTGTTGAAGTAAACAAAGAGTGCTTTTGGAGATGAACAAAAGAAGCAAGAATTTAACTCTTTCATTGTTTCTGTTTTTTCTGCTCTCTTTGCTGTCTTCTTCAGTGGTTACAGTGGAGGCAAGGAAGAACCATCACAACAAGAAGGGCAAGCATCAGAAGCAAAAGGGTGTTGGTGATGGCAATGGTTCAGGCCCTGCACCAGCTCCATTTCCTGATTTCAATCAAACTCAATCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAACGGAGTTTCTGATGATTCCAAGGTAAAAATTGTCCAGCACAACCTTACAAAACAGATACAGAGAGAGAGAGAGAGAGAGAGTTAAAAATAGTGACTAATGGACTGATTTGAACTAATAAAAACCATCCTATGCAGGCACTTCTAGCAGCATGGAAAGCTGCATGCAAGATTACTGGAGCTACGTTGCAAATACCTTCACAATATAAGTTTCTCATCAAACCAATAACTCTAAATGGCCCTTGTATGCCTCAGCTTGTTCTTCAGGTTTGCCTTATTTTCTTCTCTTTTCAGGAATTTGGCTTATTTGAGAACGTAACAAAAAAAAAACAAAAAAAAAAAATCTTTGACTTGTCATGTAACAGATTGTTTTTTAAAAATAAATAAGTTAGTGCTGCTTAGAGTTCAAAAGATAAGATAATTAATAATTTGATGGGAAGTGCAATTCCAAAGCAGATTGATGGAGCTGTATTGGCTCCTCCAAAAGTGAGTTCGTGGTCCAAATCCAGCTTGTTTCAATGGCTGAATTTCAAATGGCTACATAATTTCACCATTCAAGGCTCTGGTACTGTGGATGGCGAGGGCTTTAATTGGTGGACTCTCTCCACATTCTACAACAATAACAAGGTTAGTTCTTTTTTTTTTTTAATAATTCATTAACTTATGCTTATTACATTTAAGAGAGTGATTTTAGCTCAACCATTTGTTGTTTTTCTTGTTTCTGCAGAAGAAATTGAAGCACATTCCAGACATGAAGCCTACTGTAAACTTTTCTCACTCTTTCTCTCTCTTTTTGTTTAGATTTACAGCTTTTTTCTGAATTTTGATTGGGATTTTTTTAATACTTTGTTCATAAATGACAGGCTTTAAGATTTTATTCCAGTTACAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAATGCCATCTGAAATTTGACAACTCCGGCACCGTCAAAGTCGACAATATCACCATTTCTTCGCCGGAGAACAGCCCGAACACCGACGGCATTCACCTGCAGAACACACAAGATGTCGAAATTCAACACTCCAACATTGGCTGTGGTAGGAACCGAGCCAATTTACAACCCCTTTTTTATAATATTTTATTGTCTTTTCTAAAGTCAACCTTTTCACTGGATCTTTTCTGGTCTGTTTTATAATTTTGAGGACACAATAAAATGAGTTTTTAAGTAGAAAATTAAACTTAATTGTCTAGCAAAATGTCTAGATTATTGTTAGTCCAAGAAAATTCTAGATCTTATAATTTTTTCCTTCGGAAATTAAATGCATATCTTTTATATCAAACTATCTATCACGCAATGTCTACTATATTAAATTTTCTCTCTACCATTAAAGCAATATTGTGAGGTAAAAATAACTCCATAGGGTTAAAAAAGTATCTCCATTTAACAATATTTTGAGGAAAATATTTGGGTGCAGCTTGCTGCTCACTTCATGACCACATGAAAAAAAAAATGGTTTTTTTTTTTAAACAGAAAAAATTTGTAGATGAAAATAGAGCTGTAGAATACTTGGATGCAGCAATTTTGCTCGTATTTTGATTGCAATAGACATAAAGCTACACCACAAAGAAACTGGACTACACACTTGAACCAACAAACTGGATTACACAACTTATTTTAAGATTTGTTATTTTGGCAGGTGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCATATTCATCATATCAATTGTGGCCCTGGACATGGCATAAGGTAAATAAATCCTCATCAATTTCACTTTTTCATCAAGGCAATTCCTGTGGAGAATATTATTTATGGCCGTGATTATAAATTAACCATTTTGTTCGACTTTTCTTAAACATTATTATATTCAGCACTAAAGGTTAATATCTTGCCTATAATTACAGCTTGGGTGGACTTGGGAAAGATAAAGGTGTTGCATGTGTCTCCAATATTGTTGTCGAAAACATCTCAATTCAAAACACTCTTTCAGGAGTGAGAATCAAGACCTGGCAGGTAAAAAAAAGGTTAAACTCTAAAATTTACTCCTTGAATTATGGTGGCTGACAGGCTGTGTTATTTATTTCTTAAAACTTTTAAAAATTTCAATTGTGTATCAACATTAAATTTATTATTTTATGTAATGTTTCTGTGAGTACTTTTAAATTTTTAATGGAACGATCATTATAACACACCTATGTTGAATAAGTATAGATTGATTTGATACAATGCAGTCGAAGAAAGTAAATTTGTGGTCAAAATAAAGTGGCACGGTTTACACAAGTGATTTCATACATCACTTAAAAAAGAGAGTATAAATATGGTATACATATCTTTTTATTTTGTCCAACGATCCACTTTCTTTTGCCATTTGCACATCTAATAAAGCTGAAACCGTCAACATTTATTTAATAAACATGTCATGTTATCATTTGTTCTGCATAGTATAGGAATTACGTAGAATCACACCCCACTAATATATATATATATATATATATTTTTAATATGAACAGGGAGGAGTTGGGTCAGTGAAGAATGTATCATTCTCCAACATCCAAGTTTCTGATGTAAAGGTGCCAATTATGATAGACCAATACTATTGCGACAAGTACACATGCAAGAACCAAACAGGAGCAGTTGCAATTTCAGGTATAACATACGATCAAATCATAGGAAGTTACACAGTGCAGCCTATTCACTTAGCCTGCAGCAACCAAGTCCCATGTATAGACGTTGATCTCATAGACATCCAGCTGAATCCATCTTCAGCATCCAGAGGCTTTCAAAACTCTCTGTGCTGGAACTCTTATGGTAAATCTCAAGCTCCCCTTGTTCCTTCTAGCATAGACTACTGCTTGAGAAGGGGCAGTGGCGCTGTTAGAAGAATAGCAAGGTCCCATGATCATGTCTGCTTCTAAATGCTCTCAACTTTTCTCTGTTTTCTTTCTCTAGCTTCCCTTCTGCAGATTTTTTTTGGAAAATGTAGGAAGGTGATGATTGTGAAAGCATTTATGGCTTATCGTGTTTGGCGTTGACATATTGTAGTCAGAAATCATATATGAAATCCATTAGTATCTCATTTCCCAATTAGTCCAAGCAAAGTGATCAGAATTTCCCCTACCCATCTGTCAAAAAAGTTTCTTTTTTACTTTTTCAGGTAAGTTTTTGCCTTTTTGGAGTTGAAATGAGTTAAACTGCTGGGGGCTGGTGGGGTCCTACTCTGTTCTGTTCTGTTCTATTCTATTCTGTTCTCTTTGCCTTTGGTAAATTGGTTGGTGGAGAAGAAAGAAAGGGCTATAAGAGAGAGTTTAATGGCGCTGATAGTGGAGGAAGGCTTGTGAGGAATCAATTGCATGCGCGACATGGCTTTTGTTCTTTTTACCTTTGTAATTAATATCAAATTTTACAGGCTTTTATGGCTTCTTCAGCTGCTTATATTTTCGTTCTTAAGCGTTGGCCTCCACTTATACTGACCTTTACACGCACTCTGTTAGTGCTCTGTTGTCTTCTAGTTGTATCTAACCGAGAAGTTCTAGTACCTTCCATTTGGGGTTGGGAAGAACCAAAAGGGAAGAAGGCTGTCTCTTTCTTTATAATAATTTCTGGGTAAAATACATTTTTCGGTCCTTTTCAACTTAA

mRNA sequence

CCAAATTCCATTTCAACCCCGACCACAAACTGGTAGAGCAGAGAGAGGAAAGAAGAAAATCGCCGTAGTGGGCACCGACCGCCTCATCGAGTGGCCCACAGCGTGCGACGTCGCTAACCAGCTTCGTAGATAGATTCTAATGGAGGTTTCCAGTTCCATCAACGTGCGACCCATTTTCTGTTTCTTCTTCAAACCCCTTCTTTATACAACTTTCCAGTGGTTACAGTGGAGGCAAGGAAGAACCATCACAACAAGAAGGGCAAGCATCAGAAGCAAAAGGGTGTTGGTGATGGCAATGGTTCAGGCCCTGCACCAGCTCCATTTCCTGATTTCAATCAAACTCAATCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAACGGAGTTTCTGATGATTCCAAGGCACTTCTAGCAGCATGGAAAGCTGCATGCAAGATTACTGGAGCTACGTTGCAAATACCTTCACAATATAAGTTTCTCATCAAACCAATAACTCTAAATGGCCCTTGTATGCCTCAGCTTGTTCTTCAGATTGATGGAGCTGTATTGGCTCCTCCAAAAGTGAGTTCGTGGTCCAAATCCAGCTTGTTTCAATGGCTGAATTTCAAATGGCTACATAATTTCACCATTCAAGGCTCTGGTACTGTGGATGGCGAGGGCTTTAATTGGTGGACTCTCTCCACATTCTACAACAATAACAAGAAGAAATTGAAGCACATTCCAGACATGAAGCCTACTGCTTTAAGATTTTATTCCAGTTACAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAATGCCATCTGAAATTTGACAACTCCGGCACCGTCAAAGTCGACAATATCACCATTTCTTCGCCGGAGAACAGCCCGAACACCGACGGCATTCACCTGCAGAACACACAAGATGTCGAAATTCAACACTCCAACATTGGCTGTGGTGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCATATTCATCATATCAATTGTGGCCCTGGACATGGCATAAGCTTGGGTGGACTTGGGAAAGATAAAGGTGTTGCATGTGTCTCCAATATTGTTGTCGAAAACATCTCAATTCAAAACACTCTTTCAGGAGTGAGAATCAAGACCTGGCAGGGAGGAGTTGGGTCAGTGAAGAATGTATCATTCTCCAACATCCAAGTTTCTGATGTAAAGGTGCCAATTATGATAGACCAATACTATTGCGACAAGTACACATGCAAGAACCAAACAGGAGCAGTTGCAATTTCAGGTATAACATACGATCAAATCATAGGAAGTTACACAGTGCAGCCTATTCACTTAGCCTGCAGCAACCAAGTCCCATGTATAGACGTTGATCTCATAGACATCCAGCTGAATCCATCTTCAGCATCCAGAGGCTTTCAAAACTCTCTGTGCTGGAACTCTTATGGTAAATCTCAAGCTCCCCTTGTTCCTTCTAGCATAGACTACTGCTTGAGAAGGGGCAGTGGCGCTGTTAGAAGAATAGCAAGGTCCCATGATCATGTCTGCTTCTAAATGCTCTCAACTTTTCTCTGTTTTCTTTCTCTAGCTTCCCTTCTGCAGATTTTTTTTGGAAAATGTAGGAAGGTGATGATTGTGAAAGCATTTATGGCTTATCGTGTTTGGCGTTGACATATTGTAGTCAGAAATCATATATGAAATCCATTAGTATCTCATTTCCCAATTAGTCCAAGCAAAGTGATCAGAATTTCCCCTACCCATCTGTCAAAAAAGTTTCTTTTTTACTTTTTCAGGTAAGTTTTTGCCTTTTTGGAGTTGAAATGAGTTAAACTGCTGGGGGCTGGTGGGGTCCTACTCTGTTCTGTTCTGTTCTATTCTATTCTGTTCTCTTTGCCTTTGGTAAATTGGTTGGTGGAGAAGAAAGAAAGGGCTATAAGAGAGAGTTTAATGGCGCTGATAGTGGAGGAAGGCTTGTGAGGAATCAATTGCATGCGCGACATGGCTTTTGTTCTTTTTACCTTTGTAATTAATATCAAATTTTACAGGCTTTTATGGCTTCTTCAGCTGCTTATATTTTCGTTCTTAAGCGTTGGCCTCCACTTATACTGACCTTTACACGCACTCTGTTAGTGCTCTGTTGTCTTCTAGTTGTATCTAACCGAGAAGTTCTAGTACCTTCCATTTGGGGTTGGGAAGAACCAAAAGGGAAGAAGGCTGTCTCTTTCTTTATAATAATTTCTGGGTAAAATACATTTTTCGGTCCTTTTCAACTTAA

Coding sequence (CDS)

ATGCCTCAGCTTGTTCTTCAGATTGATGGAGCTGTATTGGCTCCTCCAAAAGTGAGTTCGTGGTCCAAATCCAGCTTGTTTCAATGGCTGAATTTCAAATGGCTACATAATTTCACCATTCAAGGCTCTGGTACTGTGGATGGCGAGGGCTTTAATTGGTGGACTCTCTCCACATTCTACAACAATAACAAGAAGAAATTGAAGCACATTCCAGACATGAAGCCTACTGCTTTAAGATTTTATTCCAGTTACAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAATGCCATCTGAAATTTGACAACTCCGGCACCGTCAAAGTCGACAATATCACCATTTCTTCGCCGGAGAACAGCCCGAACACCGACGGCATTCACCTGCAGAACACACAAGATGTCGAAATTCAACACTCCAACATTGGCTGTGGTGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCATATTCATCATATCAATTGTGGCCCTGGACATGGCATAAGCTTGGGTGGACTTGGGAAAGATAAAGGTGTTGCATGTGTCTCCAATATTGTTGTCGAAAACATCTCAATTCAAAACACTCTTTCAGGAGTGAGAATCAAGACCTGGCAGGGAGGAGTTGGGTCAGTGAAGAATGTATCATTCTCCAACATCCAAGTTTCTGATGTAAAGGTGCCAATTATGATAGACCAATACTATTGCGACAAGTACACATGCAAGAACCAAACAGGAGCAGTTGCAATTTCAGGTATAACATACGATCAAATCATAGGAAGTTACACAGTGCAGCCTATTCACTTAGCCTGCAGCAACCAAGTCCCATGTATAGACGTTGATCTCATAGACATCCAGCTGAATCCATCTTCAGCATCCAGAGGCTTTCAAAACTCTCTGTGCTGGAACTCTTATGGTAAATCTCAAGCTCCCCTTGTTCCTTCTAGCATAGACTACTGCTTGAGAAGGGGCAGTGGCGCTGTTAGAAGAATAGCAAGGTCCCATGATCATGTCTGCTTCTAA

Protein sequence

MPQLVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWTLSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSSASRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF
Homology
BLAST of Tan0017851 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 4.8e-90
Identity = 186/462 (40.26%), Postives = 256/462 (55.41%), Query Frame = 0

Query: 12  LFLFFLLSLLSSSVVTVEARKNHHNKKGKH-------------QKQKGVGDGNGSGPAPA 71
           + + +++S +     T  AR  +H  KG+H              +     D   S P P+
Sbjct: 7   ILVIYIISTVLFGHFTNVARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPS 66

Query: 72  PFPDFNQTQS-TIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKP 131
           P P    + S  +F++ SFGA G+G  DD+ A   AWKAAC +    +  P    F I  
Sbjct: 67  PDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITS 126

Query: 132 ITLNGPCMPQLVLQIDGAVLAPPKVSSW-SKSSLFQWLNFKWLHNFTIQGSGTVDGEGFN 191
              +GPC P LV Q+DG ++ P     W  K +  QWL F  L  FT  G GTV+G G  
Sbjct: 127 TIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQK 186

Query: 192 WWTLSTF-YNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKV 251
           WW L    +          P   PT +RF+ S N+ V+ + I NSPQ H+KFD    V +
Sbjct: 187 WWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLI 246

Query: 252 DNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHG 311
           + I ISSP+ SPNTDGIHL NT+ V I +S +  GDDC+SI TGCS++ I  + CGP HG
Sbjct: 247 NEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHG 306

Query: 312 ISLGGLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVP 371
           IS+G LG     ACVSNI V N  I+++ +G+R+KTWQGG GSV N+ F NIQ+ +V   
Sbjct: 307 ISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNC 366

Query: 372 IMIDQYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQ--PIHLACSNQVPCIDVDLIDIQ 431
           I++DQYYC    C+N+T AV +  + Y  I G+Y V+  PIH ACS+ V C ++ + +++
Sbjct: 367 IIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVE 426

Query: 432 LNPSSASRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAV 456
           L P        +  CWN+YGK +   +P  ID CL  GS  V
Sbjct: 427 LLPEEGEL-VDDPFCWNAYGKQETLTIP-PID-CLLDGSPVV 465

BLAST of Tan0017851 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.0e-80
Identity = 153/375 (40.80%), Postives = 224/375 (59.73%), Query Frame = 0

Query: 70  IFNILSFGAKGNGVSDDSKALLAAWKAACKITGAT-LQIPSQYKFLIKPITLNGPCMPQL 129
           + ++ +FGAKGNGV+DD+KA   AWK AC     T + +P  Y  L++PI L+GPC  +L
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARL 111

Query: 130 VLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWTLSTFYNNNK 189
            LQI G ++AP     W   +  +WL F  L   T++G GTV+G G  WW  S  +N++ 
Sbjct: 112 TLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN 171

Query: 190 KKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPN 249
                     PTAL F+   N+ V ++ +I+S Q H+   +   V +  + + +P  SPN
Sbjct: 172 PCRG-----APTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPN 231

Query: 250 TDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKGVA 309
           TDGIH+  ++ + I ++ +  GDDC+SI    + I I +I CGPGHGIS+G LGK K   
Sbjct: 232 TDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWE 291

Query: 310 CVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD-KYT 369
            V +I V+   I +T +GVRIKTWQGG G V  + F NI++++V  PI+IDQYYCD +  
Sbjct: 292 EVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKP 351

Query: 370 CKNQTGAVAISGITYDQIIG-SYTVQPIHLACSNQVPCIDVDLIDIQLNPSSASRGFQNS 429
           C NQT A++I  I++  + G S + + I ++CS+  PC ++ L DI L PS+   GF  S
Sbjct: 352 CANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGD-GFTES 411

Query: 430 LCWNSYGKSQAPLVP 442
            CW +YG S   + P
Sbjct: 412 FCWEAYGSSSGQVYP 420

BLAST of Tan0017851 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 4.4e-75
Identity = 150/397 (37.78%), Postives = 228/397 (57.43%), Query Frame = 0

Query: 70  IFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPITLNGPCMPQLV 129
           IFN+  +GA G+G  D ++A   AW+AACK   A L +P   KF++  +  NGPC P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 130 LQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWTLSTFYNNNKK 189
            ++DG + A    +SW  + +  WL F  L  FT+ G G +DG+G  WW     + N ++
Sbjct: 118 FKVDGIIAAYQNPASWKNNRI--WLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGRE 177

Query: 190 KLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNT 249
                   +PTA++F  S  + ++ + ++NSP+ HL F N   VK+  I+I++P +SPNT
Sbjct: 178 ICNDRD--RPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSPNT 237

Query: 250 DGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKGVAC 309
           DGI +  +++  +Q + IG GDDCV+I TG SNI I  + CGPGHGIS+G LG++   A 
Sbjct: 238 DGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAE 297

Query: 310 VSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYT-C 369
           VS + V      +T +G+RIKTWQGG G   ++ + N+++ + + PI+I+Q+YC   + C
Sbjct: 298 VSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASAC 357

Query: 370 KNQTGAVAISGITYDQIIG-SYTVQPIHLACSNQVPCIDVDLIDIQLNPSSASRGFQNSL 429
           +NQ  AV I  +TY  I G S T   I L CS+ +PC D+ L DI L  +S      + L
Sbjct: 358 QNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLKLTSGK--IASCL 417

Query: 430 CWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH 465
             N+ G     ++P+    C      A R+ ++SH H
Sbjct: 418 NDNANGYFSGHVIPA----CKNLSPSAKRKESKSHKH 444

BLAST of Tan0017851 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.8e-74
Identity = 139/353 (39.38%), Postives = 209/353 (59.21%), Query Frame = 0

Query: 68  STIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPITLNGPCMPQ 127
           +T+FN+  +GA G+G  D ++A    W AACK   A L +P+  KF +  +   GPC P 
Sbjct: 56  ATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPH 115

Query: 128 LVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWTLSTFYNNN 187
           L  ++DG ++A P  + W  S +  WL F  L +F + G+G +DG+G  WW       N 
Sbjct: 116 LSFKVDGTIVAQPDPARWKNSKI--WLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKVVNG 175

Query: 188 KKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSP 247
           +         +PTA++   S +VTV+++T++NSP+ HL F     VK+  + I +P +SP
Sbjct: 176 RTVCN--DRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSP 235

Query: 248 NTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKGV 307
           NTDGI +  ++   I+   IG GDDC++I TG SNI I  + CGPGHGIS+G LG+D   
Sbjct: 236 NTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSR 295

Query: 308 ACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYT 367
           A VS++ V      +T +G+RIKTWQGG G    +++ N+++ + + PI+I+Q+YC   +
Sbjct: 296 AEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSAS 355

Query: 368 -CKNQTGAVAISGITYDQIIG-SYTVQPIHLACSNQVPCIDVDLIDIQLNPSS 419
            C+NQ  AV I G+TY  I G S T   I L CS+ VPC  + L ++ L  +S
Sbjct: 356 ACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLTS 404

BLAST of Tan0017851 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.8e-73
Identity = 146/365 (40.00%), Postives = 216/365 (59.18%), Query Frame = 0

Query: 68  STIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPITLNGPCMPQ 127
           S   N+  FGAKG+G  DD+KA   AWKAAC  T + + +  +  +L++PI+ +GPC   
Sbjct: 87  SKTVNVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSG 146

Query: 128 LVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWTLSTFYNNN 187
           L +QI G + A    S + K     WL F  + N  ++G GT++G G  WW       N+
Sbjct: 147 LTMQIYGTIEASDDRSDYRKDGR-HWLVFDSVQNLRVEGGGTINGNGKIWW------QNS 206

Query: 188 KKKLKHIP-DMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENS 247
            K  K +P    PTAL FY S +V V+++ I N+ Q H+ FDN   V+  N+ +++PENS
Sbjct: 207 CKTNKALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENS 266

Query: 248 PNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKG 307
           PNTDGIH+  TQ++ I    IG GDDC+SI  G   + ++ I CGPGHGIS+G LG    
Sbjct: 267 PNTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNS 326

Query: 308 VACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDK- 367
            A VS++VV    +  T +GVRIKTWQGG GS  N+ F N+++ +V+ PI+IDQ YCD+ 
Sbjct: 327 EAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQD 386

Query: 368 YTCKNQTGAVAISGITYDQIIGSYTVQ-PIHLACSNQVPC--IDVDLIDIQLNPSSASRG 427
             C+ Q+ AV +  + Y  I G+      I   CS + PC  I ++ +D+++   +A++ 
Sbjct: 387 KPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKA 443

BLAST of Tan0017851 vs. NCBI nr
Match: XP_022966725.1 (polygalacturonase At1g48100 [Cucurbita maxima])

HSP 1 Score: 856.7 bits (2212), Expect = 9.5e-245
Identity = 421/468 (89.96%), Postives = 437/468 (93.38%), Query Frame = 0

Query: 1   MNKRSKNLTLSLFLFFLLSLL-SSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAP 60
           MNK SK LTL     FL+SLL SSSVV +EARK+HHNKK KHQKQ+G G G+GS PAPAP
Sbjct: 1   MNKGSKILTL-----FLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  FPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPIT 120
            PD+NQTQSTIFNILSFGAKGNGV DDSKAL+AAW AACKI GATLQIP  YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPIT 120

Query: 121 LNGPCMPQLVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWT 180
           LNGPCMPQLVLQIDG VLAPPKVSSWSKSSLFQWLNFKWLHNFTI GSGTVDG+GFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWT 180

Query: 181 LSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNIT 240
           +ST Y NNKKK KH+PDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VKVDNIT
Sbjct: 181 VSTLY-NNKKKGKHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNIT 240

Query: 241 ISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG 300
           ISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG
Sbjct: 241 ISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG 300

Query: 301 GLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360
           GLGKDK VACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID
Sbjct: 301 GLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360

Query: 361 QYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSSA 420
           QYYCDKY CKNQTGAVAISGI YDQIIGSYTVQPIHLACS+Q+PCIDVDLI IQLNPSS 
Sbjct: 361 QYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSG 420

Query: 421 SRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF 468
           SRGFQ++LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH C+
Sbjct: 421 SRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

BLAST of Tan0017851 vs. NCBI nr
Match: XP_023542214.1 (polygalacturonase At1g48100 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 855.1 bits (2208), Expect = 2.8e-244
Identity = 421/468 (89.96%), Postives = 436/468 (93.16%), Query Frame = 0

Query: 1   MNKRSKNLTLSLFLFFLLSLL-SSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAP 60
           MNK SK LTL     FL+SLL SSSVV +EARK+HHNKK KHQKQKG   G+GS PAPAP
Sbjct: 1   MNKGSKILTL-----FLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGAKGSGSSPAPAP 60

Query: 61  FPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPIT 120
            PD+NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKI GATLQIPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWT 180
           LNGPCMPQLVLQIDG VLAPPKVSSWSKSSLFQWLNFKWLHNFTI GSGTVDG+GFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWT 180

Query: 181 LSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNIT 240
           +ST Y NNKKK KH+PDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKVDNIT
Sbjct: 181 VSTLY-NNKKKGKHVPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVDNIT 240

Query: 241 ISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG 300
           ISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLG
Sbjct: 241 ISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLG 300

Query: 301 GLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360
           GLGKDK  ACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID
Sbjct: 301 GLGKDKSAACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360

Query: 361 QYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSSA 420
           QYYCDKY CKNQTGAVAISGI YDQIIGSYTVQPIHLACS+Q+PCIDVDLI IQLNPSS 
Sbjct: 361 QYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSG 420

Query: 421 SRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF 468
           SRGFQ++LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH C+
Sbjct: 421 SRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

BLAST of Tan0017851 vs. NCBI nr
Match: XP_022944899.1 (polygalacturonase At1g48100 isoform X4 [Cucurbita moschata])

HSP 1 Score: 854.4 bits (2206), Expect = 4.7e-244
Identity = 421/468 (89.96%), Postives = 437/468 (93.38%), Query Frame = 0

Query: 1   MNKRSKNLTLSLFLFFLLSLL-SSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAP 60
           MNK SK LTL     FL+SLL SSSVV +EARK+HHNKK KHQKQ+G G G+GS PAPAP
Sbjct: 1   MNKGSKILTL-----FLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  FPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPIT 120
            PD+NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKI GATLQIPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWT 180
           LNGPCMPQLVLQIDG VLAPPKVSSWSKSSLFQWLNFKWLHNFTI G GTVDG+GFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWT 180

Query: 181 LSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNIT 240
           +ST Y NNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NIT
Sbjct: 181 VSTLY-NNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNIT 240

Query: 241 ISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG 300
           ISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLG
Sbjct: 241 ISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLG 300

Query: 301 GLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360
           GLGKDK VACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID
Sbjct: 301 GLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360

Query: 361 QYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSSA 420
           QYYCDKY CKNQTGAVAISGI YDQIIGSYTVQPIHLACS+Q+PCIDVDLI IQLNPSS 
Sbjct: 361 QYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSG 420

Query: 421 SRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF 468
           SRGFQ++LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH C+
Sbjct: 421 SRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

BLAST of Tan0017851 vs. NCBI nr
Match: XP_023542213.1 (polygalacturonase At1g48100 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.5 bits (2196), Expect = 6.8e-243
Identity = 421/469 (89.77%), Postives = 436/469 (92.96%), Query Frame = 0

Query: 1   MNKRSKNLTLSLFLFFLLSLL-SSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAP 60
           MNK SK LTL     FL+SLL SSSVV +EARK+HHNKK KHQKQKG   G+GS PAPAP
Sbjct: 1   MNKGSKILTL-----FLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGAKGSGSSPAPAP 60

Query: 61  FPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPIT 120
            PD+NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKI GATLQIPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVL-QIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWW 180
           LNGPCMPQLVL QIDG VLAPPKVSSWSKSSLFQWLNFKWLHNFTI GSGTVDG+GFNWW
Sbjct: 121 LNGPCMPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWW 180

Query: 181 TLSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNI 240
           T+ST Y NNKKK KH+PDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKVDNI
Sbjct: 181 TVSTLY-NNKKKGKHVPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVDNI 240

Query: 241 TISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISL 300
           TISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISL
Sbjct: 241 TISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISL 300

Query: 301 GGLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMI 360
           GGLGKDK  ACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMI
Sbjct: 301 GGLGKDKSAACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMI 360

Query: 361 DQYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSS 420
           DQYYCDKY CKNQTGAVAISGI YDQIIGSYTVQPIHLACS+Q+PCIDVDLI IQLNPSS
Sbjct: 361 DQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSS 420

Query: 421 ASRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF 468
            SRGFQ++LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH C+
Sbjct: 421 GSRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 463

BLAST of Tan0017851 vs. NCBI nr
Match: XP_022944898.1 (polygalacturonase At1g48100 isoform X3 [Cucurbita moschata])

HSP 1 Score: 849.7 bits (2194), Expect = 1.2e-242
Identity = 421/469 (89.77%), Postives = 437/469 (93.18%), Query Frame = 0

Query: 1   MNKRSKNLTLSLFLFFLLSLL-SSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAP 60
           MNK SK LTL     FL+SLL SSSVV +EARK+HHNKK KHQKQ+G G G+GS PAPAP
Sbjct: 1   MNKGSKILTL-----FLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  FPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPIT 120
            PD+NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKI GATLQIPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVL-QIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWW 180
           LNGPCMPQLVL QIDG VLAPPKVSSWSKSSLFQWLNFKWLHNFTI G GTVDG+GFNWW
Sbjct: 121 LNGPCMPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWW 180

Query: 181 TLSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNI 240
           T+ST Y NNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NI
Sbjct: 181 TVSTLY-NNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNI 240

Query: 241 TISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISL 300
           TISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISL
Sbjct: 241 TISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISL 300

Query: 301 GGLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMI 360
           GGLGKDK VACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMI
Sbjct: 301 GGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMI 360

Query: 361 DQYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSS 420
           DQYYCDKY CKNQTGAVAISGI YDQIIGSYTVQPIHLACS+Q+PCIDVDLI IQLNPSS
Sbjct: 361 DQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSS 420

Query: 421 ASRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF 468
            SRGFQ++LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH C+
Sbjct: 421 GSRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 463

BLAST of Tan0017851 vs. ExPASy TrEMBL
Match: A0A6J1HSE8 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.6e-245
Identity = 421/468 (89.96%), Postives = 437/468 (93.38%), Query Frame = 0

Query: 1   MNKRSKNLTLSLFLFFLLSLL-SSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAP 60
           MNK SK LTL     FL+SLL SSSVV +EARK+HHNKK KHQKQ+G G G+GS PAPAP
Sbjct: 1   MNKGSKILTL-----FLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  FPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPIT 120
            PD+NQTQSTIFNILSFGAKGNGV DDSKAL+AAW AACKI GATLQIP  YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPIT 120

Query: 121 LNGPCMPQLVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWT 180
           LNGPCMPQLVLQIDG VLAPPKVSSWSKSSLFQWLNFKWLHNFTI GSGTVDG+GFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWT 180

Query: 181 LSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNIT 240
           +ST Y NNKKK KH+PDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VKVDNIT
Sbjct: 181 VSTLY-NNKKKGKHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNIT 240

Query: 241 ISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG 300
           ISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG
Sbjct: 241 ISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG 300

Query: 301 GLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360
           GLGKDK VACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID
Sbjct: 301 GLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360

Query: 361 QYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSSA 420
           QYYCDKY CKNQTGAVAISGI YDQIIGSYTVQPIHLACS+Q+PCIDVDLI IQLNPSS 
Sbjct: 361 QYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSG 420

Query: 421 SRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF 468
           SRGFQ++LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH C+
Sbjct: 421 SRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

BLAST of Tan0017851 vs. ExPASy TrEMBL
Match: A0A6J1FZF6 (polygalacturonase At1g48100 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.3e-244
Identity = 421/468 (89.96%), Postives = 437/468 (93.38%), Query Frame = 0

Query: 1   MNKRSKNLTLSLFLFFLLSLL-SSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAP 60
           MNK SK LTL     FL+SLL SSSVV +EARK+HHNKK KHQKQ+G G G+GS PAPAP
Sbjct: 1   MNKGSKILTL-----FLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  FPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPIT 120
            PD+NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKI GATLQIPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWT 180
           LNGPCMPQLVLQIDG VLAPPKVSSWSKSSLFQWLNFKWLHNFTI G GTVDG+GFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWT 180

Query: 181 LSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNIT 240
           +ST Y NNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NIT
Sbjct: 181 VSTLY-NNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNIT 240

Query: 241 ISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG 300
           ISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLG
Sbjct: 241 ISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLG 300

Query: 301 GLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360
           GLGKDK VACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID
Sbjct: 301 GLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 360

Query: 361 QYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSSA 420
           QYYCDKY CKNQTGAVAISGI YDQIIGSYTVQPIHLACS+Q+PCIDVDLI IQLNPSS 
Sbjct: 361 QYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSG 420

Query: 421 SRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF 468
           SRGFQ++LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH C+
Sbjct: 421 SRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

BLAST of Tan0017851 vs. ExPASy TrEMBL
Match: A0A6J1FZB5 (polygalacturonase At1g48100 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 5.6e-243
Identity = 421/469 (89.77%), Postives = 437/469 (93.18%), Query Frame = 0

Query: 1   MNKRSKNLTLSLFLFFLLSLL-SSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAP 60
           MNK SK LTL     FL+SLL SSSVV +EARK+HHNKK KHQKQ+G G G+GS PAPAP
Sbjct: 1   MNKGSKILTL-----FLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  FPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPIT 120
            PD+NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKI GATLQIPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVL-QIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWW 180
           LNGPCMPQLVL QIDG VLAPPKVSSWSKSSLFQWLNFKWLHNFTI G GTVDG+GFNWW
Sbjct: 121 LNGPCMPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWW 180

Query: 181 TLSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNI 240
           T+ST Y NNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NI
Sbjct: 181 TVSTLY-NNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNI 240

Query: 241 TISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISL 300
           TISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISL
Sbjct: 241 TISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISL 300

Query: 301 GGLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMI 360
           GGLGKDK VACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMI
Sbjct: 301 GGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMI 360

Query: 361 DQYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSS 420
           DQYYCDKY CKNQTGAVAISGI YDQIIGSYTVQPIHLACS+Q+PCIDVDLI IQLNPSS
Sbjct: 361 DQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSS 420

Query: 421 ASRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF 468
            SRGFQ++LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH C+
Sbjct: 421 GSRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 463

BLAST of Tan0017851 vs. ExPASy TrEMBL
Match: A0A6J1FZF8 (polygalacturonase At1g48100 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 8.1e-242
Identity = 421/479 (87.89%), Postives = 437/479 (91.23%), Query Frame = 0

Query: 1   MNKRSKNLTLSLFLFFLLSLL-SSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAP 60
           MNK SK LTL     FL+SLL SSSVV +EARK+HHNKK KHQKQ+G G G+GS PAPAP
Sbjct: 1   MNKGSKILTL-----FLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  FPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPIT 120
            PD+NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKI GATLQIPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWT 180
           LNGPCMPQLVLQIDG VLAPPKVSSWSKSSLFQWLNFKWLHNFTI G GTVDG+GFNWWT
Sbjct: 121 LNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWT 180

Query: 181 LSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNIT 240
           +ST Y NNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NIT
Sbjct: 181 VSTLY-NNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNIT 240

Query: 241 ISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGI--- 300
           ISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGI   
Sbjct: 241 ISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISTR 300

Query: 301 --------SLGGLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQ 360
                   SLGGLGKDK VACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQ
Sbjct: 301 GLFLAYNYSLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQ 360

Query: 361 VSDVKVPIMIDQYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVD 420
           VSDVKVPIMIDQYYCDKY CKNQTGAVAISGI YDQIIGSYTVQPIHLACS+Q+PCIDVD
Sbjct: 361 VSDVKVPIMIDQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVD 420

Query: 421 LIDIQLNPSSASRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF 468
           LI IQLNPSS SRGFQ++LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH C+
Sbjct: 421 LIGIQLNPSSGSRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 473

BLAST of Tan0017851 vs. ExPASy TrEMBL
Match: A0A6J1FZB4 (polygalacturonase At1g48100 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.0e-240
Identity = 421/480 (87.71%), Postives = 437/480 (91.04%), Query Frame = 0

Query: 1   MNKRSKNLTLSLFLFFLLSLL-SSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAP 60
           MNK SK LTL     FL+SLL SSSVV +EARK+HHNKK KHQKQ+G G G+GS PAPAP
Sbjct: 1   MNKGSKILTL-----FLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAP 60

Query: 61  FPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPIT 120
            PD+NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKI GATLQIPS YKFLIKPIT
Sbjct: 61  LPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPIT 120

Query: 121 LNGPCMPQLVL-QIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWW 180
           LNGPCMPQLVL QIDG VLAPPKVSSWSKSSLFQWLNFKWLHNFTI G GTVDG+GFNWW
Sbjct: 121 LNGPCMPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWW 180

Query: 181 TLSTFYNNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNI 240
           T+ST Y NNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NI
Sbjct: 181 TVSTLY-NNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNI 240

Query: 241 TISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGI-- 300
           TISSPE+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGI  
Sbjct: 241 TISSPESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGIST 300

Query: 301 ---------SLGGLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNI 360
                    SLGGLGKDK VACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNI
Sbjct: 301 RGLFLAYNYSLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNI 360

Query: 361 QVSDVKVPIMIDQYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDV 420
           QVSDVKVPIMIDQYYCDKY CKNQTGAVAISGI YDQIIGSYTVQPIHLACS+Q+PCIDV
Sbjct: 361 QVSDVKVPIMIDQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDV 420

Query: 421 DLIDIQLNPSSASRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCF 468
           DLI IQLNPSS SRGFQ++LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH C+
Sbjct: 421 DLIGIQLNPSSGSRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 474

BLAST of Tan0017851 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 617.8 bits (1592), Expect = 6.9e-177
Identity = 298/465 (64.09%), Postives = 370/465 (79.57%), Query Frame = 0

Query: 10  LSLFLFFLLSLLSSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAPFPDFNQTQST 69
           L++ L  ++   + S V+VE RK HH KK K + ++   +     PAPAP+P  N+    
Sbjct: 10  LAMLLGVIIVAAAISTVSVEGRK-HHVKKIKPKHRRHSKNTPTGSPAPAPYPSTNE---G 69

Query: 70  IFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPITLNGPCMPQLV 129
           +FNI S+GAKG+GVSDDSKAL+ AWKAACK+ G  ++IP+  +FL+K +TL GPC  + V
Sbjct: 70  VFNIFSYGAKGDGVSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVKAVTLQGPCKEETV 129

Query: 130 LQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWTLSTFYNNNKK 189
           +QI+G ++AP K+ SW  SSLFQWLNFKW+ + TIQGSGT++G G+NWW L T+    + 
Sbjct: 130 VQIEGILVAPEKIGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWNLDTYQTQTRN 189

Query: 190 KLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNT 249
             K+IP MKPTALRFYSS NVTVRDI+I+NSP CHLKFD+S  VKV+NITISSPENSPNT
Sbjct: 190 --KYIPPMKPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNITISSPENSPNT 249

Query: 250 DGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKGVAC 309
           DGIHLQNT++VEIQHSNI CGDDCVSIQTG SN+HIHHINCGPGHGIS+GGLGKDK VAC
Sbjct: 250 DGIHLQNTRNVEIQHSNIACGDDCVSIQTGSSNVHIHHINCGPGHGISIGGLGKDKSVAC 309

Query: 310 VSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYTCK 369
           VS+I+VE+ISIQNTL+GVRIKTWQGG+G VKN++FSNIQV DVKVPI+IDQYYCDK  CK
Sbjct: 310 VSDIIVEDISIQNTLAGVRIKTWQGGLGVVKNLTFSNIQVKDVKVPIVIDQYYCDKSKCK 369

Query: 370 NQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSSASRGF-----Q 429
           NQT AV+ISG+ Y+ I+GS+TVQP+ +ACSN VPC+DVDL+DI+L PS   RG      Q
Sbjct: 370 NQTRAVSISGVKYNNIVGSFTVQPVRIACSNNVPCMDVDLMDIRLRPSGGIRGLQTHQQQ 429

Query: 430 NSLCWNSYGKSQAPLVPSSIDYCLRR---GSGAVRRIARSHDHVC 467
            +LCWNSYGK+Q PLVPSSI YCLR+   G    ++++RS+D +C
Sbjct: 430 QALCWNSYGKTQGPLVPSSIGYCLRKSNIGGYYSQKVSRSYDKIC 468

BLAST of Tan0017851 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 433.0 bits (1112), Expect = 3.2e-121
Identity = 218/467 (46.68%), Postives = 295/467 (63.17%), Query Frame = 0

Query: 33  NHHNKKGKHQKQK-----GVGDGNG---SGP---APAPFPDFNQTQSTIFNILSFGAKGN 92
           NHH+K     K K        D N    SGP    P P P   Q  S +FN++ FGAKG+
Sbjct: 72  NHHHKSKPKPKPKLKTPPKAHDNNSPVVSGPPKVQPPPLPP--QKGSQVFNVMDFGAKGD 131

Query: 93  GVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPITLNGP-CMPQLVLQIDGAVLAPP 152
           G  DD+KA  AAW AACKI  + + +P +Y +L+ PI+ +GP C   +V Q+DG ++AP 
Sbjct: 132 GKCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVGPISFSGPYCQANIVFQLDGTIIAPT 191

Query: 153 KVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWTLSTFYNNNKKKL--------- 212
              +W K  L  W++F  L    +QG G +DG G  WW   + + ++  KL         
Sbjct: 192 DSKTWGK-GLMWWIDFTKLKGIKVQGKGVIDGRGSGWWQQDSPFIDSDTKLIVPLNNSAN 251

Query: 213 ------------KHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNIT 272
                       + +P +KPTALRF  S+ V V  ITI NSPQCHLKFD+   V V +I 
Sbjct: 252 QNPPMPIRSELDERMPSIKPTALRFSGSFGVEVTGITIQNSPQCHLKFDDCVGVVVHDIA 311

Query: 273 ISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLG 332
           +SSP +SPNTDGIHLQNT+DV I  + + CGDDC+SIQTGCSN+ +H++NCGPGHGIS+G
Sbjct: 312 VSSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCISIQTGCSNVFVHNVNCGPGHGISIG 371

Query: 333 GLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMID 392
            LGK+   ACVSNI V ++++ NT++GVRIKTWQGGVGSVK + FSNIQ++ V++PI I+
Sbjct: 372 SLGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQGGVGSVKGIIFSNIQLNQVQIPITIN 431

Query: 393 QYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSSA 452
           Q+YCD   CKNQT AVA+ G+TY++I G+YTV+P+H ACS+  PC+DV L  I+L P   
Sbjct: 432 QFYCDHSKCKNQTSAVAVEGVTYERIKGTYTVKPVHFACSDNFPCVDVQLSSIELKPVQE 491

Query: 453 SRGFQNSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVC 467
                ++ CW ++G+   P +P  ID CL+ G     ++   HD VC
Sbjct: 492 KYRMYDAYCWQTFGELNTPTLP-PID-CLKIGKPPRNKVQSDHD-VC 532

BLAST of Tan0017851 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 432.6 bits (1111), Expect = 4.1e-121
Identity = 212/434 (48.85%), Postives = 283/434 (65.21%), Query Frame = 0

Query: 55  PAPAPFPDFNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFL 114
           P P P P      S  FN+L FGAKG+G+SDD++A  AAW +ACK+  +T+ IP  Y FL
Sbjct: 107 PPPTPLPLQPVEDSQQFNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFL 166

Query: 115 IKPITLNGP-CMPQLVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGE 174
           + PI+ +GP C   +V Q++G ++AP    SW    L  W+ F  L   TIQG+G +DG 
Sbjct: 167 VGPISFSGPYCQANIVFQLEGMIVAPTDTESWG-GGLMWWIEFTKLSGITIQGNGVIDGR 226

Query: 175 GFNWWT---LSTFYNNNKKKL---------------------KHIPDMKPTALRFYSSYN 234
           G  WW    LS +  ++  KL                       +P +KPTALRFY S +
Sbjct: 227 GTVWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSID 286

Query: 235 VTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGC 294
           VTV  ITI NSPQCHLKFDN   V V ++ +SSP +SPNTDGIHLQNT+DV I  + + C
Sbjct: 287 VTVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLAC 346

Query: 295 GDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKGVACVSNIVVENISIQNTLSGVRI 354
           GDDC+SIQTGCSN+++H++NCGPGHGIS+G LGKD   ACVSNI V ++ + NT++GVRI
Sbjct: 347 GDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRI 406

Query: 355 KTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYTCKNQTGAVAISGITYDQIIGSY 414
           KTWQGG+GSVK + FSNIQ+++V++PI+IDQ+YCD   C N T AV++ G+TY++I G+Y
Sbjct: 407 KTWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTY 466

Query: 415 TVQPIHLACSNQVPCIDVDLIDIQLNPSSASRGFQNSLCWNSYGKSQAPLVPSSIDYCLR 464
           TV+P+H ACS+  PCIDV L  I+L P        +  CW ++G+  +  VP  ID CL+
Sbjct: 467 TVKPVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELNSATVP-PID-CLQ 526

BLAST of Tan0017851 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 402.9 bits (1034), Expect = 3.5e-112
Identity = 207/433 (47.81%), Postives = 273/433 (63.05%), Query Frame = 0

Query: 10  LSLFLFFLLSLLSSSVVTVEARKNHHNKKGKHQKQKGVGDGNGSGPAPAPFPDFNQTQST 69
           LSL    L+SL SS   T +AR + + K  +    + + +G  SG               
Sbjct: 9   LSLNFLLLISLFSSRFGTCDARYSVYWKGNR----RSIAEGGSSG--------------- 68

Query: 70  IFNILSFGAKGNGVSDDSKALLAAWKAACKITGATLQIPSQYKFLIKPITLNG-PCMPQL 129
             N+L  GAKG+G SDD+KA   AW+ ACK+  +TL +PS   FL+ P++  G  C  ++
Sbjct: 69  TINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKECKEKI 128

Query: 130 VLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSGTVDGEGFNWWTLSTFYNNNK 189
           V Q++G ++AP   S+W  S L QW+ FK L   TI+G G +DG G  WW        N 
Sbjct: 129 VFQLEGKIIAPTSASAWG-SGLLQWIEFKALQGITIKGKGIIDGRGSVWW--------ND 188

Query: 190 KKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPN 249
                +P  KPTALRFY S  VTV  ITI NSPQ HLKFDN  +++V + T SSP +SPN
Sbjct: 189 MMGTKMPRTKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTSSPGDSPN 248

Query: 250 TDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKGVA 309
           TDGIHLQN+QD  I  S + CGDDC+SIQTGCSNI+IH ++CGPGHGIS+GGLGKD   A
Sbjct: 249 TDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGGLGKDNTKA 308

Query: 310 CVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYTC 369
           CVSNI V ++++  T +GVRIK+WQGG GSVK V FSNIQVS+V  PI+IDQYYCD   C
Sbjct: 309 CVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQYYCDGGGC 368

Query: 370 KNQTGAVAISGITYDQIIGSYTVQPIHLACSNQVPCIDVDLIDIQLNPSSASRGFQNSLC 429
            N+T AVA+S I Y  I G+YT +P+  ACS+ +PC  + L  I+L P++      +  C
Sbjct: 369 HNETSAVAVSNINYINIKGTYTKEPVRFACSDSLPCTGISLSTIELKPATGKASSLDPFC 413

Query: 430 WNSYGKSQAPLVP 442
           W ++G+ +   +P
Sbjct: 429 WKAHGELKTKTLP 413

BLAST of Tan0017851 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-97
Identity = 181/403 (44.91%), Postives = 246/403 (61.04%), Query Frame = 0

Query: 55  PAPAPFPDFNQTQS---TIFNILSFGAKGNGVSDDSKALLAAWKAACKITG---ATLQIP 114
           P+PAP P +N   +   T+F++ SFGA G+  +DD+ A   AW AAC  TG   A L +P
Sbjct: 29  PSPAPNPAYNDNDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLLVP 88

Query: 115 SQYKFLIKPITLNGPCMPQLVLQIDGAVLAPPKVSSWSKSSLFQWLNFKWLHNFTIQGSG 174
             + FL+KP T NGPC   LVLQIDG +++P    SW  +   QW+ F  ++  +IQGSG
Sbjct: 89  YTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQRQWMMFYRVNGLSIQGSG 148

Query: 175 TVDGEGFNWWTLSTFYNNNKKKLKHI-PDMKPTALRFYSSYNVTVRDITIINSPQCHLKF 234
            ++G G  WW L    +          P   P A+R + S  V ++ I  +NS Q H++F
Sbjct: 149 VINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFHVRF 208

Query: 235 DNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHH 294
           DN   V VD++ I +P +SPNTDGIH++NT +V+I++S I  GDDC+SI  GC N+ I +
Sbjct: 209 DNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVDIKN 268

Query: 295 INCGPGHGISLGGLGKDKGVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNI 354
           + CGP HGIS+G LG     A VSNI V N +I N+ +GVRIKTWQGG GSV  + FSNI
Sbjct: 269 VTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVFSNI 328

Query: 355 QVSDVKVPIMIDQYYCDKYTCKNQTGAVAISGITYDQIIGSYTVQ--PIHLACSNQVPCI 414
            + +V+ PIMIDQYYC    C NQT AV IS + Y  I G+Y ++  PIH  CS+ VPC 
Sbjct: 329 LMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSVPCT 388

Query: 415 DVDLIDIQLNPSSASRGFQNSLCWNSYGKSQAPLVPSSIDYCL 449
           ++ L ++ L PS      +N  CWN+YG  +  + P    YCL
Sbjct: 389 NLTLTEVDLFPSKGQH-LENPFCWNAYGSMKITVPPV---YCL 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949Z14.8e-9040.26Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ52.0e-8040.80Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432124.4e-7537.78Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E72.8e-7439.38Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P353361.8e-7340.00Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022966725.19.5e-24589.96polygalacturonase At1g48100 [Cucurbita maxima][more]
XP_023542214.12.8e-24489.96polygalacturonase At1g48100 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022944899.14.7e-24489.96polygalacturonase At1g48100 isoform X4 [Cucurbita moschata][more]
XP_023542213.16.8e-24389.77polygalacturonase At1g48100 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022944898.11.2e-24289.77polygalacturonase At1g48100 isoform X3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1HSE84.6e-24589.96polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=... [more]
A0A6J1FZF62.3e-24489.96polygalacturonase At1g48100 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1FZB55.6e-24389.77polygalacturonase At1g48100 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1FZF88.1e-24287.89polygalacturonase At1g48100 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1FZB42.0e-24087.71polygalacturonase At1g48100 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
AT3G26610.16.9e-17764.09Pectin lyase-like superfamily protein [more]
AT1G10640.13.2e-12146.68Pectin lyase-like superfamily protein [more]
AT1G60590.14.1e-12148.85Pectin lyase-like superfamily protein [more]
AT5G14650.13.5e-11247.81Pectin lyase-like superfamily protein [more]
AT1G56710.11.1e-9744.91Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 133..154
e-value: 4000.0
score: 2.9
coord: 83..132
e-value: 46.0
score: 12.9
coord: 186..207
e-value: 15.0
score: 14.5
coord: 215..236
e-value: 2400.0
score: 4.5
coord: 156..176
e-value: 7800.0
score: 0.9
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 18..297
e-value: 1.8E-75
score: 254.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 2..326
e-value: 8.0E-94
score: 316.9
NoneNo IPR availablePANTHERPTHR31375:SF92PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 1..331
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 1..331
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 4..320

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0017851.2Tan0017851.2mRNA
Tan0017851.1Tan0017851.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity