Tan0017433 (gene) Snake gourd v1

Overview


Name	Tan0017433
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Cytochrome P450
Location	LG05: 36908940 .. 36909868 (+)
RNA-Seq Expression	Tan0017433
Synteny	Tan0017433

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTTGCCATCTATGCAAGTGTCCCAGAAAAGTAAAGGTAAATTAGAATTTTTTTTTTTGAAAGAAGTTTTAGTGAGAAAATTGAGGGAGGAAAATCTTAATTTAAGAAAAGTTATGAAGATATTTTTGAGATGAGCAGAACTACTTTGGCACAATCTTTATTTAACAAAAAAGTGTAGGTTCTATTACTTGCAGGAACGGATTCATCATCTGCCACCATAGAATGGGCAATGACTGAACTACTCAACAATCCGGAGGTGCTAAATAAAGCCATGGTCGAGTTGGACACAATAGTTGGTCAAGATCGTCTCATCAACGAAGTAGATCTCACTAGTCTCAATTATCTTCAAGCCATTGTTTTCGAGACACTTCGCTTGCACCCAGCTGCTCCGATGCTCCTCCCACATTATTCCTCCGACAACTGTACCGTCGCTGGCTATGACATTCCACGTGGCACAATGTTGTTGGTCAATGCATGGGCGATACATAGAGACCCGAAGCTTTGGGACGACCCAACGAGGTTTCGACCAGAGAGGTTTTTGGGATCAAGAAATGAGTGCCAATTGAATAAACTGATTGCTTTTGGGCTTGGAAGGAGGGCTTGTCCTGGTGCAATTATGGCTAAAAGTTTTGTCGGATTGACTTTAGGGCTATTGATTCAGTGTTATGAGTGGAAAAAGACTGGAGATGAAGAGATGGATATGACTGAAACTGGGGGTACTACTGCTCACAAAGTGAAGCCTTTGGAAGCCATGTGCAAAGCTCGCCCTATCATGAGCAAAGTTTTCTTCAAATTTCACCAGGGTTTTATATAACTTTTCTTCTTTGTTGTATATTAATAATAATGACACTTCTTTAGAATATGGGATGTGTTACCTTCCCCCCGAAAATAGATGGAATGTATTAAGAAGATTAGTTTACTTCTTTA

mRNA sequence

ATTTTTGCCATCTATGCAAGTGTCCCAGAAAAGTAAAGGTAAATTAGAATTTTTTTTTTTGAAAGAAGTTTTAGTGAGAAAATTGAGGGAGGAAAATCTTAATTTAAGAAAAGTTATGAAGATATTTTTGAGATGAGCAGAACTACTTTGGCACAATCTTTATTTAACAAAAAAGTGTAGGTTCTATTACTTGCAGGAACGGATTCATCATCTGCCACCATAGAATGGGCAATGACTGAACTACTCAACAATCCGGAGGTGCTAAATAAAGCCATGGTCGAGTTGGACACAATAGTTGGTCAAGATCGTCTCATCAACGAAGTAGATCTCACTAGTCTCAATTATCTTCAAGCCATTGTTTTCGAGACACTTCGCTTGCACCCAGCTGCTCCGATGCTCCTCCCACATTATTCCTCCGACAACTGTACCGTCGCTGGCTATGACATTCCACGTGGCACAATGTTGTTGGTCAATGCATGGGCGATACATAGAGACCCGAAGCTTTGGGACGACCCAACGAGGTTTCGACCAGAGAGGTTTTTGGGATCAAGAAATGAGTGCCAATTGAATAAACTGATTGCTTTTGGGCTTGGAAGGAGGGCTTGTCCTGGTGCAATTATGGCTAAAAGTTTTGTCGGATTGACTTTAGGGCTATTGATTCAGTGTTATGAGTGGAAAAAGACTGGAGATGAAGAGATGGATATGACTGAAACTGGGGGTACTACTGCTCACAAAGTGAAGCCTTTGGAAGCCATGTGCAAAGCTCGCCCTATCATGAGCAAAGTTTTCTTCAAATTTCACCAGGGTTTTATATAACTTTTCTTCTTTGTTGTATATTAATAATAATGACACTTCTTTAGAATATGGGATGTGTTACCTTCCCCCCGAAAATAGATGGAATGTATTAAGAAGATTAGTTTACTTCTTTA

Coding sequence (CDS)

ATGACTGAACTACTCAACAATCCGGAGGTGCTAAATAAAGCCATGGTCGAGTTGGACACAATAGTTGGTCAAGATCGTCTCATCAACGAAGTAGATCTCACTAGTCTCAATTATCTTCAAGCCATTGTTTTCGAGACACTTCGCTTGCACCCAGCTGCTCCGATGCTCCTCCCACATTATTCCTCCGACAACTGTACCGTCGCTGGCTATGACATTCCACGTGGCACAATGTTGTTGGTCAATGCATGGGCGATACATAGAGACCCGAAGCTTTGGGACGACCCAACGAGGTTTCGACCAGAGAGGTTTTTGGGATCAAGAAATGAGTGCCAATTGAATAAACTGATTGCTTTTGGGCTTGGAAGGAGGGCTTGTCCTGGTGCAATTATGGCTAAAAGTTTTGTCGGATTGACTTTAGGGCTATTGATTCAGTGTTATGAGTGGAAAAAGACTGGAGATGAAGAGATGGATATGACTGAAACTGGGGGTACTACTGCTCACAAAGTGAAGCCTTTGGAAGCCATGTGCAAAGCTCGCCCTATCATGAGCAAAGTTTTCTTCAAATTTCACCAGGGTTTTATATAA

Protein sequence

MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHYSSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGLGRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARPIMSKVFFKFHQGFI

Homology

[Close]

BLAST of Tan0017433 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 4.8e-60
Identity = 107/185 (57.84%), Postives = 138/185 (74.59%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           ++ LLN+P+V++KA  E+D  VG DRLI E DL+ L YL+ IV ETLRLHPA P+L+PH 
Sbjct: 319 LSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHM 378

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           +S++C +  YD+PRGT LLVNAWAIHRDP  WDDP  F+PERF     E +  KL+AFGL
Sbjct: 379 ASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERF---EKEEEAQKLLAFGL 438

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRRACPG+ +A+  VGL LG LIQC+EW++ G+ E+DM E  G T  K  PL+A+CKARP
Sbjct: 439 GRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARP 498

Query: 181 IMSKV 186
            + K+
Sbjct: 499 FLHKI 500

[Close]

BLAST of Tan0017433 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 8.2e-60
Identity = 109/185 (58.92%), Postives = 139/185 (75.14%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           M+ LLN+PE L KA  E++T VG +RLI E DL  L YL  I+ ET RL PAAPML+PH 
Sbjct: 312 MSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPAAPMLVPHE 371

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           SSD+C V GYD+P+GT+LLVNAWAIHRDP+ WD+PT F+PER  G   E + +KL+ FG+
Sbjct: 372 SSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGV--ELEPSKLMPFGM 431

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRR+CPG+ +A+  VGLTLG LIQC+EWK+ G+ ++DM E  G T  K +PLEA+CK R 
Sbjct: 432 GRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCKPRN 491

Query: 181 IMSKV 186
           I+ KV
Sbjct: 492 ILHKV 494

[Close]

BLAST of Tan0017433 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 2.0e-58
Identity = 104/179 (58.10%), Postives = 138/179 (77.09%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           M+ LLN+PE++ KA  ELDT +G DR ++E D++ L YLQ+IV+ETLRLH AAP+L+PH 
Sbjct: 315 MSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHL 374

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           SS++ ++ GY+IP+ T+L+VNAW IHRDP LW DPT F+PERF     E ++NKL++FGL
Sbjct: 375 SSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERF---EKEGEVNKLLSFGL 434

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKAR 180
           GRRACPG  +++   GLTLGLLIQC+EWK+ G+E++DM E  G TA K   L AMCK R
Sbjct: 435 GRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKKTSLNAMCKVR 490

[Close]

BLAST of Tan0017433 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 7.6e-58
Identity = 105/186 (56.45%), Postives = 140/186 (75.27%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           M+ LLN+PEVL KA  E+D  +G DRL+ E D+  L YLQ IV ETLRL+P APMLLPH 
Sbjct: 321 MSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHL 380

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           +S++C V GYD+PRGT++LVNAWAIHRDPKLW++P +F+PERF     + +  KL+ FG+
Sbjct: 381 ASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERF---EKKGEDKKLMPFGI 440

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTET-GGTTAHKVKPLEAMCKAR 180
           GRR+CPG+ +A+  V L LG L+QC+EW++  ++ +DM E+  GTT  K   L+AMCKAR
Sbjct: 441 GRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKAR 500

Query: 181 PIMSKV 186
           PI+ KV
Sbjct: 501 PIVHKV 503

[Close]

BLAST of Tan0017433 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.0e-57
Identity = 103/185 (55.68%), Postives = 139/185 (75.14%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           M+ELLN+PEVL KA  ELDT +G+++L++E DL+ L YLQ I+ ETLRLHP AP+LLPHY
Sbjct: 318 MSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHY 377

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           SS++CT+  +++P+ T++L N W IHRDPK W+D   F+PERF     E ++NK++AFGL
Sbjct: 378 SSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF---EKEEEVNKVMAFGL 437

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRRACPG  +A+  VG T+GLLIQC+EW++  +E++DM E  G T     PL AMCKA P
Sbjct: 438 GRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMKIPLRAMCKALP 497

Query: 181 IMSKV 186
           I + V
Sbjct: 498 IANDV 499

[Close]

BLAST of Tan0017433 vs. NCBI nr
Match: XP_022149478.1 (cytochrome P450 81D1-like [Momordica charantia])

HSP 1 Score: 302.4 bits (773), Expect = 2.9e-78
Identity = 146/188 (77.66%), Postives = 158/188 (84.04%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           MT+LLNNPEVL KA  ELD  VGQDR ++E DL +LN+LQAIV ETLRL PAAPMLL HY
Sbjct: 20  MTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIVSETLRLQPAAPMLLSHY 79

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGS-RNECQLNKLIAFG 120
           SS++CTVAGYDIPRGT LLVNAWAIHRDPKLWDDPTRFRPERFLGS  NE Q NK+IAFG
Sbjct: 80  SSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERFLGSAANESQCNKVIAFG 139

Query: 121 LGRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKAR 180
           LGRRACPG  MA  FVGLTLGLLIQCYEWKK G+EE+DM E GG T HK KPLE MCK R
Sbjct: 140 LGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGEGGGITIHKAKPLEVMCKPR 199

Query: 181 PIMSKVFF 188
           P+M K+ F
Sbjct: 200 PVMDKLQF 207

[Close]

BLAST of Tan0017433 vs. NCBI nr
Match: AEM42992.1 (cytochrome P450 [Siraitia grosvenorii])

HSP 1 Score: 298.1 bits (762), Expect = 5.4e-77
Identity = 142/185 (76.76%), Postives = 156/185 (84.32%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           M +LLNNPE+L KA  ELDT +GQDR ++E DL +L+YLQAIV ETLRLHPAAPMLL HY
Sbjct: 310 MAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAPMLLSHY 369

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           SS +CTVAGYDIPRGT LLVNAWAIHRDPKLWDDPT FRPERFLG+ NE Q  KLIAFGL
Sbjct: 370 SSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKKLIAFGL 429

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRR+CPG  MA  FVGLTLGLLIQCY+WKK GDE++DM E GG T HK KPLEAMCKARP
Sbjct: 430 GRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLEAMCKARP 489

Query: 181 IMSKV 186
            M K+
Sbjct: 490 AMYKL 494

[Close]

BLAST of Tan0017433 vs. NCBI nr
Match: XP_022149457.1 (cytochrome P450 81E8-like isoform X2 [Momordica charantia])

HSP 1 Score: 294.3 bits (752), Expect = 7.8e-76
Identity = 142/189 (75.13%), Postives = 155/189 (82.01%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           MT+LLNNPEVL KA  ELDT VGQDRL++E DL +L YLQAIVFETLRLHP APML+ HY
Sbjct: 323 MTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQAIVFETLRLHPPAPMLIAHY 382

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGS-RNECQLNKLIAFG 120
           SS +CTVAGY IP  T+LLVNAWAIHRDPKLWDDPT FRPERFLGS  NE   NKLIAFG
Sbjct: 383 SSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPERFLGSAANELLSNKLIAFG 442

Query: 121 LGRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKAR 180
           LGRRACPGA M + FVGLTL L+IQCYEWKK+G+E +DM E GG T  K KPLE MCKAR
Sbjct: 443 LGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGEGGGITILKAKPLEVMCKAR 502

Query: 181 PIMSKVFFK 189
           P+M KV  K
Sbjct: 503 PVMDKVHVK 511

[Close]

BLAST of Tan0017433 vs. NCBI nr
Match: XP_022149456.1 (cytochrome P450 81E8-like isoform X1 [Momordica charantia])

HSP 1 Score: 294.3 bits (752), Expect = 7.8e-76
Identity = 142/189 (75.13%), Postives = 155/189 (82.01%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           MT+LLNNPEVL KA  ELDT VGQDRL++E DL +L YLQAIVFETLRLHP APML+ HY
Sbjct: 326 MTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQAIVFETLRLHPPAPMLIAHY 385

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGS-RNECQLNKLIAFG 120
           SS +CTVAGY IP  T+LLVNAWAIHRDPKLWDDPT FRPERFLGS  NE   NKLIAFG
Sbjct: 386 SSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPERFLGSAANELLSNKLIAFG 445

Query: 121 LGRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKAR 180
           LGRRACPGA M + FVGLTL L+IQCYEWKK+G+E +DM E GG T  K KPLE MCKAR
Sbjct: 446 LGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGEGGGITILKAKPLEVMCKAR 505

Query: 181 PIMSKVFFK 189
           P+M KV  K
Sbjct: 506 PVMDKVHVK 514

[Close]

BLAST of Tan0017433 vs. NCBI nr
Match: XP_023512643.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 293.9 bits (751), Expect = 1.0e-75
Identity = 134/185 (72.43%), Postives = 157/185 (84.86%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           MT+LLNNPEV++KA  E+DT +G+DRL++E DLT+L+YLQAI+ ET RLHPAAPMLL HY
Sbjct: 309 MTQLLNNPEVISKAKAEIDTKIGRDRLVDEADLTNLHYLQAIISETFRLHPAAPMLLTHY 368

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           SSD+CTVAGYDIPRGTMLLVNAWAIHRDPKLWDDP RFRPERFLG  NE   NKLI FG+
Sbjct: 369 SSDDCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPMRFRPERFLGEGNELLSNKLIPFGM 428

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRRACPG  MA+  +GL+LGLLIQCY+WK  G +++DMTE GG T  KV+PLE +C+A P
Sbjct: 429 GRRACPGETMAQRLIGLSLGLLIQCYDWKNVGYQKVDMTEGGGITIQKVQPLETLCRALP 488

Query: 181 IMSKV 186
           IM KV
Sbjct: 489 IMDKV 493

[Close]

BLAST of Tan0017433 vs. ExPASy TrEMBL
Match: A0A6J1D6W7 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111017895 PE=3 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.4e-78
Identity = 146/188 (77.66%), Postives = 158/188 (84.04%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           MT+LLNNPEVL KA  ELD  VGQDR ++E DL +LN+LQAIV ETLRL PAAPMLL HY
Sbjct: 20  MTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIVSETLRLQPAAPMLLSHY 79

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGS-RNECQLNKLIAFG 120
           SS++CTVAGYDIPRGT LLVNAWAIHRDPKLWDDPTRFRPERFLGS  NE Q NK+IAFG
Sbjct: 80  SSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERFLGSAANESQCNKVIAFG 139

Query: 121 LGRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKAR 180
           LGRRACPG  MA  FVGLTLGLLIQCYEWKK G+EE+DM E GG T HK KPLE MCK R
Sbjct: 140 LGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGEGGGITIHKAKPLEVMCKPR 199

Query: 181 PIMSKVFF 188
           P+M K+ F
Sbjct: 200 PVMDKLQF 207

[Close]

BLAST of Tan0017433 vs. ExPASy TrEMBL
Match: K7NC01 (Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.6e-77
Identity = 142/185 (76.76%), Postives = 156/185 (84.32%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           M +LLNNPE+L KA  ELDT +GQDR ++E DL +L+YLQAIV ETLRLHPAAPMLL HY
Sbjct: 310 MAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIVSETLRLHPAAPMLLSHY 369

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           SS +CTVAGYDIPRGT LLVNAWAIHRDPKLWDDPT FRPERFLG+ NE Q  KLIAFGL
Sbjct: 370 SSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERFLGAANELQSKKLIAFGL 429

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRR+CPG  MA  FVGLTLGLLIQCY+WKK GDE++DM E GG T HK KPLEAMCKARP
Sbjct: 430 GRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGGGITIHKAKPLEAMCKARP 489

Query: 181 IMSKV 186
            M K+
Sbjct: 490 AMYKL 494

[Close]

BLAST of Tan0017433 vs. ExPASy TrEMBL
Match: A0A6J1D806 (cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.8e-76
Identity = 142/189 (75.13%), Postives = 155/189 (82.01%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           MT+LLNNPEVL KA  ELDT VGQDRL++E DL +L YLQAIVFETLRLHP APML+ HY
Sbjct: 323 MTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQAIVFETLRLHPPAPMLIAHY 382

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGS-RNECQLNKLIAFG 120
           SS +CTVAGY IP  T+LLVNAWAIHRDPKLWDDPT FRPERFLGS  NE   NKLIAFG
Sbjct: 383 SSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPERFLGSAANELLSNKLIAFG 442

Query: 121 LGRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKAR 180
           LGRRACPGA M + FVGLTL L+IQCYEWKK+G+E +DM E GG T  K KPLE MCKAR
Sbjct: 443 LGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGEGGGITILKAKPLEVMCKAR 502

Query: 181 PIMSKVFFK 189
           P+M KV  K
Sbjct: 503 PVMDKVHVK 511

[Close]

BLAST of Tan0017433 vs. ExPASy TrEMBL
Match: A0A6J1D8F1 (cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.8e-76
Identity = 142/189 (75.13%), Postives = 155/189 (82.01%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           MT+LLNNPEVL KA  ELDT VGQDRL++E DL +L YLQAIVFETLRLHP APML+ HY
Sbjct: 326 MTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQAIVFETLRLHPPAPMLIAHY 385

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGS-RNECQLNKLIAFG 120
           SS +CTVAGY IP  T+LLVNAWAIHRDPKLWDDPT FRPERFLGS  NE   NKLIAFG
Sbjct: 386 SSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPERFLGSAANELLSNKLIAFG 445

Query: 121 LGRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKAR 180
           LGRRACPGA M + FVGLTL L+IQCYEWKK+G+E +DM E GG T  K KPLE MCKAR
Sbjct: 446 LGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGEGGGITILKAKPLEVMCKAR 505

Query: 181 PIMSKVFFK 189
           P+M KV  K
Sbjct: 506 PVMDKVHVK 514

[Close]

BLAST of Tan0017433 vs. ExPASy TrEMBL
Match: A0A6J1J5B5 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.4e-75
Identity = 132/185 (71.35%), Postives = 157/185 (84.86%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           MT+LLNNPEV++KA  E+DT +G+DRL++E DL +L+YLQAI++ET RLHPAAPMLL HY
Sbjct: 309 MTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETFRLHPAAPMLLTHY 368

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           SSD+CTVAGYDIPRGTML+VNAWAIHRDPKLWDDP  FRPERFLG+ NE   NKL+ FG+
Sbjct: 369 SSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAGNELLSNKLVPFGM 428

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRRACPG  MA+  +GL+LGLLIQCYEWK  G +++DMTE GG T  KV+PLE MC+A P
Sbjct: 429 GRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQKVQPLETMCRALP 488

Query: 181 IMSKV 186
           IM KV
Sbjct: 489 IMDKV 493

[Close]

BLAST of Tan0017433 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 245.0 bits (624), Expect = 5.1e-65
Identity = 115/185 (62.16%), Postives = 143/185 (77.30%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           ++ LLNNPEVLNKA  E+D ++G DRL+ E D+ +L YLQ IV ETLRL+PAAPMLLPH 
Sbjct: 313 LSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHV 372

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           +S +C V GYD+PRGTMLL NAWAIHRDP LWDDPT F+PERF     E +  KL+ FGL
Sbjct: 373 ASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERF---EKEGEAKKLMPFGL 432

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRRACPG+ +A+  V L+LG LIQC+EW++ G+EE+DMTE  G T  K +PLEAMC+AR 
Sbjct: 433 GRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRARD 492

Query: 181 IMSKV 186
            + K+
Sbjct: 493 FVGKI 494

[Close]

BLAST of Tan0017433 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 237.7 bits (605), Expect = 8.1e-63
Identity = 113/186 (60.75%), Postives = 144/186 (77.42%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           ++ +LN+P+VLNKA  E+D  +G DRL++E D+++L YLQ IV ETLRL+PAAPMLLPH 
Sbjct: 312 LSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHV 371

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           +S++C VAGYD+PRGT+LL N WAIHRDP+LWDDP  F+PERF     E +  KL+ FGL
Sbjct: 372 ASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERF---EKEGEAQKLMPFGL 431

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRRACPG+ +A   + LTLG LIQC EW+K G EE+DM+E  G T  K KPLEAMC+ARP
Sbjct: 432 GRRACPGSGLAHRLINLTLGSLIQCLEWEKIG-EEVDMSEGKGVTMPKAKPLEAMCRARP 491

Query: 181 IMSKVF 187
            + K+F
Sbjct: 492 SVVKIF 493

[Close]

BLAST of Tan0017433 vs. TAIR 10
Match: AT1G66540.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 234.6 bits (597), Expect = 6.8e-62
Identity = 108/185 (58.38%), Postives = 139/185 (75.14%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           ++ LLNNPEVL K   E+D  +G DRL+ E D+ +L YLQ IV ETLRL+PA P+L+PH 
Sbjct: 200 LSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHI 259

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           SS++C V GYD+P GTMLLVN WAIHRDP+LWDDP  F+PERF     E + +KL+ FGL
Sbjct: 260 SSEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERF---EKEGETHKLLTFGL 319

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRRACPG+ +A+  V L+LG LIQC+EW++ G+EE+DMTE GG T  +  PL AMC+AR 
Sbjct: 320 GRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRARA 379

Query: 181 IMSKV 186
            + K+
Sbjct: 380 FVGKI 381

[Close]

BLAST of Tan0017433 vs. TAIR 10
Match: AT1G66540.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 234.6 bits (597), Expect = 6.8e-62
Identity = 108/185 (58.38%), Postives = 139/185 (75.14%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           ++ LLNNPEVL K   E+D  +G DRL+ E D+ +L YLQ IV ETLRL+PA P+L+PH 
Sbjct: 141 LSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHI 200

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           SS++C V GYD+P GTMLLVN WAIHRDP+LWDDP  F+PERF     E + +KL+ FGL
Sbjct: 201 SSEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERF---EKEGETHKLLTFGL 260

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRRACPG+ +A+  V L+LG LIQC+EW++ G+EE+DMTE GG T  +  PL AMC+AR 
Sbjct: 261 GRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRARA 320

Query: 181 IMSKV 186
            + K+
Sbjct: 321 FVGKI 322

[Close]

BLAST of Tan0017433 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 232.3 bits (591), Expect = 3.4e-61
Identity = 107/185 (57.84%), Postives = 138/185 (74.59%), Query Frame = 0


Query: 1   MTELLNNPEVLNKAMVELDTIVGQDRLINEVDLTSLNYLQAIVFETLRLHPAAPMLLPHY 60
           ++ LLN+P+V++KA  E+D  VG DRLI E DL+ L YL+ IV ETLRLHPA P+L+PH 
Sbjct: 319 LSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHM 378

Query: 61  SSDNCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPTRFRPERFLGSRNECQLNKLIAFGL 120
           +S++C +  YD+PRGT LLVNAWAIHRDP  WDDP  F+PERF     E +  KL+AFGL
Sbjct: 379 ASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERF---EKEEEAQKLLAFGL 438

Query: 121 GRRACPGAIMAKSFVGLTLGLLIQCYEWKKTGDEEMDMTETGGTTAHKVKPLEAMCKARP 180
           GRRACPG+ +A+  VGL LG LIQC+EW++ G+ E+DM E  G T  K  PL+A+CKARP
Sbjct: 439 GRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARP 498

Query: 181 IMSKV 186
            + K+
Sbjct: 499 FLHKI 500

The following BLAST results are available for this feature:

BLAST of Tan0017433 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FG65	4.8e-60	57.84	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
W8JMU7	8.2e-60	58.92	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ8	2.0e-58	58.10	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q9LHA1	7.6e-58	56.45	Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1	[more]
Q6WNQ9	1.0e-57	55.68	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]

BLAST of Tan0017433 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149478.1	2.9e-78	77.66	cytochrome P450 81D1-like [Momordica charantia]	[more]
AEM42992.1	5.4e-77	76.76	cytochrome P450 [Siraitia grosvenorii]	[more]
XP_022149457.1	7.8e-76	75.13	cytochrome P450 81E8-like isoform X2 [Momordica charantia]	[more]
XP_022149456.1	7.8e-76	75.13	cytochrome P450 81E8-like isoform X1 [Momordica charantia]	[more]
XP_023512643.1	1.0e-75	72.43	cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Tan0017433 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D6W7	1.4e-78	77.66	cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111017895 PE=3 SV...	[more]
K7NC01	2.6e-77	76.76	Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1	[more]
A0A6J1D806	3.8e-76	75.13	cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1D8F1	3.8e-76	75.13	cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1J5B5	1.4e-75	71.35	cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1	[more]

BLAST of Tan0017433 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37360.1	5.1e-65	62.16	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT4G37370.1	8.1e-63	60.75	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT1G66540.1	6.8e-62	58.38	Cytochrome P450 superfamily protein	[more]
AT1G66540.2	6.8e-62	58.38	Cytochrome P450 superfamily protein	[more]
AT5G36220.1	3.4e-61	57.84	cytochrome p450 81d1	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 116..125 score: 51.99 coord: 6..19 score: 34.92 coord: 41..52 score: 41.87
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..161 e-value: 2.4E-49 score: 168.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 115..125 score: 49.59 coord: 40..58 score: 37.71 coord: 125..148 score: 29.55 coord: 81..105 score: 42.63
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..181 e-value: 6.9E-58 score: 198.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..179
None	No IPR available	PANTHER	PTHR24298:SF683	CYTOCHROME P450 FAMILY PROTEIN	coord: 1..185
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 1..185
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 118..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0017433.1	Tan0017433.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen