Tan0017311 (gene) Snake gourd v1

Overview


Name	Tan0017311
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily
Location	LG05: 67983143 .. 67984203 (-)
RNA-Seq Expression	Tan0017311
Synteny	Tan0017311

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTTCATTGTTTCCGTTGGCAGCATTTTTTTTAATGCTATTGTATCGTTAGACGGAAGCGGAAACTTCAAAAGTATTGGAGCTGCTATAGCAGCTGCACCAAACAATAGCAAGTCCAGGTTTGGAATATATAAGGAGCAGGTTGAAGTTGAGAGTCAAAAGACTTTCATTGCATTAATAGGTGAAAATGCAGCCACCACCGTCATAGTTGATAATAGGAGCAATGGCACTGGTTTTGGAACTTCTTACACTGCCACTTTCGGTAAGCATGAGTTATACCCAACTTTTTCTTTTATCTTCTTCTTTCAATCCCTTCATTTCAATATTAATTATTATATGTTGAACTTTTTGACATTTCATTTGCAGTTGTGAAGGGTAACAACTTTATGGCACAATCCATAACATTTAAAAATTCTGCTGGACCTGAAAATGGCCAAGCAGTTGCCCTTCTTAACAACGGTGCCAACTATGCGGTGTACTACAAATGCGTATTTCTTGGTTTTCAGGACACACTTTTCGTGAATGGAGACAATTTATTTTTCAAAAACTGTGAAATCTATGGAACGGTAGATTTCATATTTGGGAATGGGCGCGCTTTGTATCAAGATTGTAAGATATATGCAAGATTAGCCAATACTACTATTACCATTACTGCACAATCTAAAGAATTCCAAAGCCAAAGAAGTGGCTTCACCTTCCAAAATTGCAGTGTAATGGCCTCTCCTGAATTTGGATCAAGAAAACGTCTCGATTTTGAAGTTTATCTTGGTCGACCTTGGAGAGATTATTCAACTGTAATTTTCATGGAGTCATTTTTGGATTTTGTGGTCAATCCAGCGGGATGGTCACCTTGGTCGACGGGAAGACCTTTAAATTTATTATTTTATGCAGAATATAACAATAGAGGCCCAGGAGCTGATTTAACTAGAAGAGTCAAATGGCCAGGTTTCCATGTAATAAGTAATCCAGAAGAAGCCACTAATTTTACGGTGAAATTGTTCATTAATGGATACACATGGTTACCAGAAACCGGTGTGCCATTTAGGCCTACCTTATAG

mRNA sequence

ATGAACTTCATTGTTTCCGTTGGCAGCATTTTTTTTAATGCTATTGTATCGTTAGACGGAAGCGGAAACTTCAAAAGTATTGGAGCTGCTATAGCAGCTGCACCAAACAATAGCAAGTCCAGGTTTGGAATATATAAGGAGCAGGTTGAAGTTGAGAGTCAAAAGACTTTCATTGCATTAATAGGTGAAAATGCAGCCACCACCGTCATAGTTGATAATAGGAGCAATGGCACTGGTTTTGGAACTTCTTACACTGCCACTTTCGTTGTGAAGGGTAACAACTTTATGGCACAATCCATAACATTTAAAAATTCTGCTGGACCTGAAAATGGCCAAGCAGTTGCCCTTCTTAACAACGGTGCCAACTATGCGGTGTACTACAAATGCGTATTTCTTGGTTTTCAGGACACACTTTTCGTGAATGGAGACAATTTATTTTTCAAAAACTGTGAAATCTATGGAACGGTAGATTTCATATTTGGGAATGGGCGCGCTTTGTATCAAGATTGTAAGATATATGCAAGATTAGCCAATACTACTATTACCATTACTGCACAATCTAAAGAATTCCAAAGCCAAAGAAGTGGCTTCACCTTCCAAAATTGCAGTGTAATGGCCTCTCCTGAATTTGGATCAAGAAAACGTCTCGATTTTGAAGTTTATCTTGGTCGACCTTGGAGAGATTATTCAACTGTAATTTTCATGGAGTCATTTTTGGATTTTGTGGTCAATCCAGCGGGATGGTCACCTTGGTCGACGGGAAGACCTTTAAATTTATTATTTTATGCAGAATATAACAATAGAGGCCCAGGAGCTGATTTAACTAGAAGAGTCAAATGGCCAGGTTTCCATGTAATAAGTAATCCAGAAGAAGCCACTAATTTTACGGTGAAATTGTTCATTAATGGATACACATGGTTACCAGAAACCGGTGTGCCATTTAGGCCTACCTTATAG

Coding sequence (CDS)

ATGAACTTCATTGTTTCCGTTGGCAGCATTTTTTTTAATGCTATTGTATCGTTAGACGGAAGCGGAAACTTCAAAAGTATTGGAGCTGCTATAGCAGCTGCACCAAACAATAGCAAGTCCAGGTTTGGAATATATAAGGAGCAGGTTGAAGTTGAGAGTCAAAAGACTTTCATTGCATTAATAGGTGAAAATGCAGCCACCACCGTCATAGTTGATAATAGGAGCAATGGCACTGGTTTTGGAACTTCTTACACTGCCACTTTCGTTGTGAAGGGTAACAACTTTATGGCACAATCCATAACATTTAAAAATTCTGCTGGACCTGAAAATGGCCAAGCAGTTGCCCTTCTTAACAACGGTGCCAACTATGCGGTGTACTACAAATGCGTATTTCTTGGTTTTCAGGACACACTTTTCGTGAATGGAGACAATTTATTTTTCAAAAACTGTGAAATCTATGGAACGGTAGATTTCATATTTGGGAATGGGCGCGCTTTGTATCAAGATTGTAAGATATATGCAAGATTAGCCAATACTACTATTACCATTACTGCACAATCTAAAGAATTCCAAAGCCAAAGAAGTGGCTTCACCTTCCAAAATTGCAGTGTAATGGCCTCTCCTGAATTTGGATCAAGAAAACGTCTCGATTTTGAAGTTTATCTTGGTCGACCTTGGAGAGATTATTCAACTGTAATTTTCATGGAGTCATTTTTGGATTTTGTGGTCAATCCAGCGGGATGGTCACCTTGGTCGACGGGAAGACCTTTAAATTTATTATTTTATGCAGAATATAACAATAGAGGCCCAGGAGCTGATTTAACTAGAAGAGTCAAATGGCCAGGTTTCCATGTAATAAGTAATCCAGAAGAAGCCACTAATTTTACGGTGAAATTGTTCATTAATGGATACACATGGTTACCAGAAACCGGTGTGCCATTTAGGCCTACCTTATAG

Protein sequence

MNFIVSVGSIFFNAIVSLDGSGNFKSIGAAIAAAPNNSKSRFGIYKEQVEVESQKTFIALIGENAATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAVYYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARLANTTITITAQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVNPAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFINGYTWLPETGVPFRPTL

Homology

[Close]

BLAST of Tan0017311 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.6e-74
Identity = 141/311 (45.34%), Postives = 197/311 (63.34%), Query Frame = 0


Query: 12  FNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENA 71
           +N  V++DG+GNF +I  A+ AAPN S++RF      G Y E VE+  +KT I  IG+  
Sbjct: 220 YNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGI 279

Query: 72  ATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAV 131
             TVI  NRS   G+ T  T T  VKG  ++A+ I+F NSAGP   QAVA   +G++++ 
Sbjct: 280 GKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVA-FRSGSDHSA 339

Query: 132 YYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARLANT--TITI 191
           +Y+C F G+QDTL+V+    F++ C+IYGT+DFIFGN   ++Q+  +YAR  N    I  
Sbjct: 340 FYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAF 399

Query: 192 TAQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVV 251
           TAQS+    Q +G +  NC ++A+P+    K  +F+ YLGRPWR YS  + ++SF+D ++
Sbjct: 400 TAQSRNQSDQPTGISILNCRILAAPDLIPVKE-NFKAYLGRPWRKYSRTVIIKSFIDDLI 459

Query: 252 NPAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFING 311
           +PAGW        L  L+Y EY N GPGA++ +RV WPGF  I N  EAT FTV  FI+G
Sbjct: 460 HPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDG 519

Query: 312 YTWLPETGVPF 315
            TWL  TG+PF
Sbjct: 520 STWLNSTGIPF 528

[Close]

BLAST of Tan0017311 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.1e-72
Identity = 137/309 (44.34%), Postives = 197/309 (63.75%), Query Frame = 0


Query: 13  NAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENAA 72
           N +V+ DGSGN K+I  A+AAA     SR+      G Y E +EV+ +   I  +G+   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 73  TTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAVY 132
            T+I  ++S G G  T  +AT  V G+NF+A+ IT +N+AGP N QAVA L +G++ +V+
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVA-LRSGSDLSVF 321

Query: 133 YKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARL-ANTTITITA 192
           Y+C F G+QDTL+V+    F++ C+IYGTVDFIFGN   + Q+C I+AR   N T T+TA
Sbjct: 322 YRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTA 381

Query: 193 QSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVNP 252
           Q +   +Q +G    NC V A+ +     +   + +LGRPW+ YS  +++++FLD ++NP
Sbjct: 382 QGRTDPNQSTGIIIHNCRVTAASDL-KPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINP 441

Query: 253 AGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFINGYT 312
           AGW  WS    LN L+YAEY N GPG+    RVKW G+HV+++P + + FTV  FI G +
Sbjct: 442 AGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNS 501

Query: 313 WLPETGVPF 315
           WLP T VPF
Sbjct: 502 WLPATNVPF 506

[Close]

BLAST of Tan0017311 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 9.9e-71
Identity = 136/311 (43.73%), Postives = 198/311 (63.67%), Query Frame = 0


Query: 16  VSLDGSGNFKSIGAAIAAAPNNSKS---------RFGIYKEQVEVESQKTFIALIGENAA 75
           VS DG+GNF +I AA+AAAPNN+             GIY+E + +   K ++ +IG+   
Sbjct: 267 VSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGIN 326

Query: 76  TTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAVY 135
            TV+  NRS   G+ T  +ATF V   NF+A +ITF+N+AGPE  QAVA L +GA+++++
Sbjct: 327 QTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVA-LRSGADFSIF 386

Query: 136 YKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYAR--LANTTITIT 195
           Y C F  +QDTL+ +    F++ C++YGTVDFIFGN   ++Q+C +Y R  + N    IT
Sbjct: 387 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 446

Query: 196 AQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVN 255
           AQ +   +Q +G + QNC++  + +  S      + YLGRPW++YS  ++M+S++D  V 
Sbjct: 447 AQGRSDPNQNTGTSIQNCTIKPADDLVS-SNYTVKTYLGRPWKEYSRTVYMQSYIDGFVE 506

Query: 256 PAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVK-LFING 315
           P GW  W+    L+ L+YAEYNN GPG++ T RV WPG+HVI N  +A NFTV  LFI  
Sbjct: 507 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI-NSTDAANFTVTGLFIEA 566

[Close]

BLAST of Tan0017311 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-70
Identity = 134/309 (43.37%), Postives = 194/309 (62.78%), Query Frame = 0


Query: 15  IVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENAATT 74
           +V+ DG+GNF +I  AI+ APN S  R       G+Y E +++   KT I LIG+ +  T
Sbjct: 239 VVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVT 298

Query: 75  VIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAVYYK 134
            I  NRS G G+ T  +AT  V G  F+A+ I   N+AGPE  QAVAL  N A++   Y+
Sbjct: 299 FITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVN-ADFVALYR 358

Query: 135 CVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARL--ANTTITITAQ 194
           CV  G+QDTL+ +    F++ C+IYGT+D+IFGN   ++Q C I ++L        ITAQ
Sbjct: 359 CVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQ 418

Query: 195 SKEFQSQRSGFTFQNCSVMASPE-FGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVNP 254
           S++ Q + +G + QNCS++AS + F S  ++  + YLGRPWR++S  + MES++D  ++ 
Sbjct: 419 SRDTQDEDTGISMQNCSILASEDLFNSSNKV--KSYLGRPWREFSRTVVMESYIDEFIDG 478

Query: 255 AGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFINGYT 314
           +GWS W+ G  L+ L+Y EYNN GPG++  +RV WPGFH++   E+A NFT   FI G  
Sbjct: 479 SGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGY-EDAFNFTATEFITGDG 538

[Close]

BLAST of Tan0017311 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 3.7e-70
Identity = 132/310 (42.58%), Postives = 199/310 (64.19%), Query Frame = 0


Query: 13  NAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENAA 72
           N +V+ +G+GN+ +IG AI+AAPN+S++RF      G Y E +E+  +KT I  IG+   
Sbjct: 207 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 266

Query: 73  TTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAVY 132
            TVI  NRS   G+   ++AT  V+G+ F+A+ ++F N AGPE  QAVA L + ++ + Y
Sbjct: 267 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVA-LRSSSDLSAY 326

Query: 133 YKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARLANTT--ITIT 192
           Y+C F  +QDT++V+    F++ C+IYGTVDFIFG+   ++Q+C +YAR  N    I  T
Sbjct: 327 YRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYT 386

Query: 193 AQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVN 252
           AQ +E   + +G +  +  ++A+P+     + +F+ YLGRPW+ YS  + M+SF+D +V+
Sbjct: 387 AQGRENSREPTGISIISSRILAAPDL-IPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVD 446

Query: 253 PAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFINGY 312
           PAGW  W     L  L+Y EY N GPG+++T RV+WPGF  I   EEA+ F+V  FI+G 
Sbjct: 447 PAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGN 506

Query: 313 TWLPETGVPF 315
            WL  T +PF
Sbjct: 507 KWLNSTRIPF 514

[Close]

BLAST of Tan0017311 vs. NCBI nr
Match: KAG6584281.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 435.3 bits (1118), Expect = 4.6e-118
Identity = 218/325 (67.08%), Postives = 256/325 (78.77%), Query Frame = 0


Query: 3   FIVSVGSIFFNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKT 62
           F  +   I +NA VSLDG G++ +IGAAIAAAPNNS SRF      G+Y E +++ES KT
Sbjct: 13  FCTTSSCISYNATVSLDGKGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKT 72

Query: 63  FIALIGENAATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNS--AGPENGQAV 122
           FI L G++A+TT IV NRS  TGFGT+ +ATFVV  N+F+A SITF NS  AGP++GQAV
Sbjct: 73  FITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAV 132

Query: 123 ALLN-NGANYAVYYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIY 182
           ALLN N  NY V+YKCVFLG+QDTLF+NG  LFFK+CEIYGTVDFIFGN   ++QDC+IY
Sbjct: 133 ALLNSNNENYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIY 192

Query: 183 ARLANTTITITAQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVI 242
           ARL N T+T+TAQSKE++ Q SGF+FQNCSVMASPE G     +  V+LGRPWRDYSTVI
Sbjct: 193 ARLLNNTVTVTAQSKEYKRQLSGFSFQNCSVMASPELGPVN--ETIVFLGRPWRDYSTVI 252

Query: 243 FMESFLDFVVNPAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEAT 302
           FMESFLD VVNPAGWS WS    L LLFYAEY NRGPGA+L+ RVKWPGFH I N +EAT
Sbjct: 253 FMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYGNRGPGANLSGRVKWPGFHAIENRKEAT 312

Query: 303 NFTVKLFINGYTWLPETGVPFRPTL 319
            F+V+ FING TWLPETGVP+RP L
Sbjct: 313 RFSVRQFINGTTWLPETGVPYRPDL 335

[Close]

BLAST of Tan0017311 vs. NCBI nr
Match: KAG6584282.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 433.7 bits (1114), Expect = 1.4e-117
Identity = 219/325 (67.38%), Postives = 253/325 (77.85%), Query Frame = 0


Query: 3   FIVSVGSIFFNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKT 62
           F  +   I +NA VSLDG G++ +IGAAIAAAPNNS SRF      G+Y E + +ES KT
Sbjct: 13  FCTTSSCISYNATVSLDGKGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIRIESSKT 72

Query: 63  FIALIGENAATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNS--AGPENGQAV 122
           FI L G++A+TT IV NRS  TGFGT+ +ATFVV  N+F+A SITF NS  AGP +GQAV
Sbjct: 73  FITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPNHGQAV 132

Query: 123 ALLN-NGANYAVYYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIY 182
           ALLN N  NY V+YKCVFLG+QDTLF+NG  LFFK+CEIYGTVDFIFGN   ++QDC+IY
Sbjct: 133 ALLNSNNVNYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIY 192

Query: 183 ARLANTTITITAQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVI 242
           ARL N T+T+TAQSKE Q Q SGF+FQNCSVMASPE G     +  V+LGRPWRDYSTVI
Sbjct: 193 ARLLNNTVTVTAQSKENQLQLSGFSFQNCSVMASPELGPVN--ETIVFLGRPWRDYSTVI 252

Query: 243 FMESFLDFVVNPAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEAT 302
           FMESFLD VVNPAGWS WS    L LLFYAEY NRGPGA+L+ RVKWPGFH I N +EAT
Sbjct: 253 FMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYGNRGPGANLSGRVKWPGFHAIENRKEAT 312

Query: 303 NFTVKLFINGYTWLPETGVPFRPTL 319
            F+V+ FING TWLPETGVP+RP L
Sbjct: 313 RFSVRQFINGTTWLPETGVPYRPDL 335

[Close]

BLAST of Tan0017311 vs. NCBI nr
Match: KAG7019877.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 416.0 bits (1068), Expect = 2.9e-112
Identity = 207/307 (67.43%), Postives = 244/307 (79.48%), Query Frame = 0


Query: 21  SGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENAATTVIVDNR 80
           +G++ +IGAAIAAAPNNS SRF      G+Y E +++ES KTFI L G++A+TT IV NR
Sbjct: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145

Query: 81  SNGTGFGTSYTATFVVKGNNFMAQSITFKNS--AGPENGQAVALLN-NGANYAVYYKCVF 140
           S  TGFGT+ +ATFVV  N+F+A SITF NS  AGP++GQAVALLN N  NY V+YKCVF
Sbjct: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205

Query: 141 LGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARLANTTITITAQSKEFQ 200
           LG+QDTLF+NG  LFFK+CEIYGTVDFIFGN   ++QDC+IYARL N T+T+TAQSK+  
Sbjct: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNI 265

Query: 201 SQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVNPAGWSPW 260
            Q SGF+FQNCSVM SPE G     +  V+LGRPWRDYSTVIFMESFLD VVNPAGWS W
Sbjct: 266 KQLSGFSFQNCSVMVSPELGPVN--ETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEW 325

Query: 261 STGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFINGYTWLPETG 319
           S    L LLFYAEY+NRGPGA+L+ RVKWPGFH I N +EAT F+V+ FING TWLPETG
Sbjct: 326 SNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETG 385

[Close]

BLAST of Tan0017311 vs. NCBI nr
Match: XP_022137308.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 405.6 bits (1041), Expect = 3.9e-109
Identity = 205/322 (63.66%), Postives = 245/322 (76.09%), Query Frame = 0


Query: 3   FIVSVGSIFFNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKT 62
           F+V  G+  FNA VSLDGSG++K+I  AIAAAP+NS +RF      G Y E VE+ S KT
Sbjct: 19  FVVGHGNTVFNATVSLDGSGDYKTIKDAIAAAPDNSITRFYIHVMAGTYNEHVEIPSTKT 78

Query: 63  FIALIGENAATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVAL 122
           F+ALIG++A+TT+IVDNRSN  GF T ++ATF VKG NFMA+SITFKNSAGPENGQAVAL
Sbjct: 79  FVALIGDDASTTIIVDNRSNRIGFKTFHSATFNVKGRNFMAKSITFKNSAGPENGQAVAL 138

Query: 123 LNNGANYAVYYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARL 182
           LN+ A+  VYY C+FLGFQDTL+V GD                        +DCKIY  L
Sbjct: 139 LND-AHQTVYYNCIFLGFQDTLYVRGD------------------------KDCKIYVGL 198

Query: 183 ANTTITITAQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFME 242
           +NTT+TITAQSKE +S+ SGF+FQNCSV  SPE G RK  +F+VYLGRP RDYSTVIFM+
Sbjct: 199 SNTTVTITAQSKELESEHSGFSFQNCSVTTSPELGPRKDQNFDVYLGRPSRDYSTVIFMQ 258

Query: 243 SFLDFVVNPAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFT 302
           SFLD VVNP GW  WS G PLNLLFYAE+NNRGPGAD+++R+KW G+H+I+NP EAT +T
Sbjct: 259 SFLDTVVNPKGWLEWSLGSPLNLLFYAEFNNRGPGADISKRIKWQGYHLINNPLEATKYT 315

Query: 303 VKLFINGYTWLPETGVPFRPTL 319
           VK FI+G TWLPET VPFRP+L
Sbjct: 319 VKDFIDGTTWLPETNVPFRPSL 315

[Close]

BLAST of Tan0017311 vs. NCBI nr
Match: XP_022150332.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 391.7 bits (1005), Expect = 5.9e-105
Identity = 196/313 (62.62%), Postives = 231/313 (73.80%), Query Frame = 0


Query: 12  FNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENA 71
           FNA VS DGSGNF  +  AIAAAPN S +RF      GIYKE VEV  QKTFIALIGE+A
Sbjct: 29  FNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYIHVKPGIYKEHVEVPVQKTFIALIGEDA 88

Query: 72  ATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAV 131
           +TT+IVDNRSNG+GF T  TAT +VKG NFMAQ +TF+NSA  + GQA+A+++  A +  
Sbjct: 89  STTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFENSAEQKEGQAIAVMDQ-AKHTA 148

Query: 132 YYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARLANTTITITA 191
           YYKCVFL +QDTL+      FFK C+IYG +DFIFGNG  ++QDC IYAR+  T I +TA
Sbjct: 149 YYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGLVVFQDCNIYARIPGTHIIVTA 208

Query: 192 QSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVNP 251
           QSK     +SGF FQNC+V  SPE  S K  +  ++LGRPWRDYS VIFMESFLD VV P
Sbjct: 209 QSKNAVEDQSGFIFQNCNVTVSPEIASSKD-NVTMFLGRPWRDYSMVIFMESFLDRVVQP 268

Query: 252 AGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFINGYT 311
            GW  W  GRP+NLLFYAEYNNRGPGAD ++RV WPG+HV+ N  E T FTV+ FING  
Sbjct: 269 KGWLEW-PGRPVNLLFYAEYNNRGPGADTSQRVDWPGYHVLHNAMEVTKFTVENFINGTK 328

Query: 312 WLPETGVPFRPTL 319
           WLPETG+PFR  L
Sbjct: 329 WLPETGIPFRAGL 338

[Close]

BLAST of Tan0017311 vs. ExPASy TrEMBL
Match: A0A6J1C9Z6 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008805 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.9e-109
Identity = 205/322 (63.66%), Postives = 245/322 (76.09%), Query Frame = 0


Query: 3   FIVSVGSIFFNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKT 62
           F+V  G+  FNA VSLDGSG++K+I  AIAAAP+NS +RF      G Y E VE+ S KT
Sbjct: 19  FVVGHGNTVFNATVSLDGSGDYKTIKDAIAAAPDNSITRFYIHVMAGTYNEHVEIPSTKT 78

Query: 63  FIALIGENAATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVAL 122
           F+ALIG++A+TT+IVDNRSN  GF T ++ATF VKG NFMA+SITFKNSAGPENGQAVAL
Sbjct: 79  FVALIGDDASTTIIVDNRSNRIGFKTFHSATFNVKGRNFMAKSITFKNSAGPENGQAVAL 138

Query: 123 LNNGANYAVYYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARL 182
           LN+ A+  VYY C+FLGFQDTL+V GD                        +DCKIY  L
Sbjct: 139 LND-AHQTVYYNCIFLGFQDTLYVRGD------------------------KDCKIYVGL 198

Query: 183 ANTTITITAQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFME 242
           +NTT+TITAQSKE +S+ SGF+FQNCSV  SPE G RK  +F+VYLGRP RDYSTVIFM+
Sbjct: 199 SNTTVTITAQSKELESEHSGFSFQNCSVTTSPELGPRKDQNFDVYLGRPSRDYSTVIFMQ 258

Query: 243 SFLDFVVNPAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFT 302
           SFLD VVNP GW  WS G PLNLLFYAE+NNRGPGAD+++R+KW G+H+I+NP EAT +T
Sbjct: 259 SFLDTVVNPKGWLEWSLGSPLNLLFYAEFNNRGPGADISKRIKWQGYHLINNPLEATKYT 315

Query: 303 VKLFINGYTWLPETGVPFRPTL 319
           VK FI+G TWLPET VPFRP+L
Sbjct: 319 VKDFIDGTTWLPETNVPFRPSL 315

[Close]

BLAST of Tan0017311 vs. ExPASy TrEMBL
Match: A0A6J1DAG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 2.9e-105
Identity = 196/313 (62.62%), Postives = 231/313 (73.80%), Query Frame = 0


Query: 12  FNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENA 71
           FNA VS DGSGNF  +  AIAAAPN S +RF      GIYKE VEV  QKTFIALIGE+A
Sbjct: 29  FNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYIHVKPGIYKEHVEVPVQKTFIALIGEDA 88

Query: 72  ATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAV 131
           +TT+IVDNRSNG+GF T  TAT +VKG NFMAQ +TF+NSA  + GQA+A+++  A +  
Sbjct: 89  STTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFENSAEQKEGQAIAVMDQ-AKHTA 148

Query: 132 YYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARLANTTITITA 191
           YYKCVFL +QDTL+      FFK C+IYG +DFIFGNG  ++QDC IYAR+  T I +TA
Sbjct: 149 YYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGLVVFQDCNIYARIPGTHIIVTA 208

Query: 192 QSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVNP 251
           QSK     +SGF FQNC+V  SPE  S K  +  ++LGRPWRDYS VIFMESFLD VV P
Sbjct: 209 QSKNAVEDQSGFIFQNCNVTVSPEIASSKD-NVTMFLGRPWRDYSMVIFMESFLDRVVQP 268

Query: 252 AGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFINGYT 311
            GW  W  GRP+NLLFYAEYNNRGPGAD ++RV WPG+HV+ N  E T FTV+ FING  
Sbjct: 269 KGWLEW-PGRPVNLLFYAEYNNRGPGADTSQRVDWPGYHVLHNAMEVTKFTVENFINGTK 328

Query: 312 WLPETGVPFRPTL 319
           WLPETG+PFR  L
Sbjct: 329 WLPETGIPFRAGL 338

[Close]

BLAST of Tan0017311 vs. ExPASy TrEMBL
Match: A0A6J1D381 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 1.4e-104
Identity = 194/312 (62.18%), Postives = 238/312 (76.28%), Query Frame = 0


Query: 10  IFFNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGE 69
           I FNA VSLDGSG++  I  AIAAAPNN   RF      GIYKE +EV   KTFI+LIG+
Sbjct: 27  ITFNATVSLDGSGDYVHISDAIAAAPNNGSIRFYIHVKPGIYKEHIEVPELKTFISLIGD 86

Query: 70  NAATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANY 129
           +A+TTVIVD+RSN  GF T+ +ATF VK  +F+AQ +TF+NSAGPENGQAVA+L+  ANY
Sbjct: 87  DASTTVIVDDRSNHAGFSTTSSATFTVKAKHFVAQFLTFQNSAGPENGQAVAVLSE-ANY 146

Query: 130 AVYYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARLANTTITI 189
             YYKCVF+G+QDTL+V G    FK C++YG++DFIFG+G A++QDC +YAR+  T  TI
Sbjct: 147 TAYYKCVFMGYQDTLYVRGAFQLFKECDVYGSIDFIFGDGLAIFQDCNVYARILGT--TI 206

Query: 190 TAQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVV 249
           TAQSK ++ QRSGFTFQNC+V  SPE  S K     V LGRPWR+YSTVIFMESFLD +V
Sbjct: 207 TAQSKSYKDQRSGFTFQNCNVTVSPEIQSIKD-QVTVSLGRPWRNYSTVIFMESFLDDIV 266

Query: 250 NPAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFING 309
            P GWS W    P+ LLFYAEYNN G GAD ++RVKWPG+HV+ N +EA+ FTV++FI+G
Sbjct: 267 EPGGWSEWPR-TPVYLLFYAEYNNSGSGADTSQRVKWPGYHVLHNEDEASRFTVEIFIDG 326

Query: 310 YTWLPETGVPFR 316
             WL ETG+PFR
Sbjct: 327 LEWLSETGIPFR 333

[Close]

BLAST of Tan0017311 vs. ExPASy TrEMBL
Match: A0A6J1GK92 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 7.0e-104
Identity = 201/320 (62.81%), Postives = 240/320 (75.00%), Query Frame = 0


Query: 1   MNFIVSVGSIFFNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQ 60
           +NF+  V S  FNA V+LDG+GNFKSI  AIAAAPNNS +RF      GIY E +E+ S 
Sbjct: 15  LNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRVSPGIYHEHLEIASA 74

Query: 61  KTFIALIGENAATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAV 120
           K FIALIGEN  TT+IVDNRSN  GF TS +AT VVKGNNFMAQ ++F+NSA  ++GQAV
Sbjct: 75  KKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHLSFENSADHQDGQAV 134

Query: 121 ALLNNGANYAVYYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYA 180
           A+L++ A Y VYYKC FL FQDTL+  G+  FFK C+IYGTVDFIFGNG A++QDC +YA
Sbjct: 135 AVLDD-AKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFGNGLAMFQDCNVYA 194

Query: 181 RLANTTITITAQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIF 240
           RL +   T+TAQSK+ Q  +SGF+FQNC+V  SPE  S K L  ++YLGRPW+ YSTV+F
Sbjct: 195 RLPSNDSTVTAQSKK-QWTQSGFSFQNCTVTVSPEIASCKAL-VKIYLGRPWKPYSTVVF 254

Query: 241 MESFLDFVVNPAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATN 300
           MESFLD  V P GW  W  G P+N LFYAEYNNRGPGA+ T RV WPGFHVI   + A +
Sbjct: 255 MESFLDDNVQPQGWMLW-PGVPVNNLFYAEYNNRGPGANTTHRVNWPGFHVIDR-QLAKS 314

Query: 301 FTVKLFINGYTWLPETGVPF 315
           FTV+ FING  WLPET VP+
Sbjct: 315 FTVENFINGTYWLPETNVPY 329

[Close]

BLAST of Tan0017311 vs. ExPASy TrEMBL
Match: A0A6J1GM54 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 7.8e-103
Identity = 198/323 (61.30%), Postives = 241/323 (74.61%), Query Frame = 0


Query: 2   NFIVSVGSIFFNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQK 61
           N +  V SI FNA+VSLDGSG+FKSI  AIAAAPNNS +RF      GIY+  +E+   K
Sbjct: 16  NVLALVDSIRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRVTPGIYRGHLEIPPAK 75

Query: 62  TFIALIGENAATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVA 121
            FIALIG++A TT+IVDNRS+GTGF TS +AT VV G+NFMAQ ++F+NSA P+NGQAVA
Sbjct: 76  KFIALIGDSALTTIIVDNRSHGTGFKTSESATLVVNGDNFMAQHLSFENSAEPQNGQAVA 135

Query: 122 LLNNGANYAVYYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYAR 181
           +L+  A ++VYY C F+ FQDTL+  G+  FF+ C+IYGTVDFIFGNG A++QDC IYAR
Sbjct: 136 VLDY-AKHSVYYDCRFMSFQDTLYSKGEFQFFRECDIYGTVDFIFGNGLAVFQDCNIYAR 195

Query: 182 LANTTITITAQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFM 241
           L    IT+TAQSKE    +SGF+FQNC+V  SPE  + K    ++YLGRPWR+YSTV+FM
Sbjct: 196 LPMDEITVTAQSKEQSVSQSGFSFQNCTVTVSPEITANK-TKVKIYLGRPWREYSTVVFM 255

Query: 242 ESFLDFVVNPAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNF 301
           ESFLD  V P GW  W  G P+N LFYAE+NNRGPGA+ T RV WP FHV+ +   A NF
Sbjct: 256 ESFLDDNVQPQGWISWE-GVPINNLFYAEFNNRGPGANTTHRVNWPTFHVL-DKLLAKNF 315

Query: 302 TVKLFINGYTWLPETGVPFRPTL 319
           TV+ FING  WLPET VPFR  L
Sbjct: 316 TVENFINGSDWLPETHVPFRTGL 334

[Close]

BLAST of Tan0017311 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 279.3 bits (713), Expect = 4.0e-75
Identity = 141/311 (45.34%), Postives = 197/311 (63.34%), Query Frame = 0


Query: 12  FNAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENA 71
           +N  V++DG+GNF +I  A+ AAPN S++RF      G Y E VE+  +KT I  IG+  
Sbjct: 220 YNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGI 279

Query: 72  ATTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAV 131
             TVI  NRS   G+ T  T T  VKG  ++A+ I+F NSAGP   QAVA   +G++++ 
Sbjct: 280 GKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVA-FRSGSDHSA 339

Query: 132 YYKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARLANT--TITI 191
           +Y+C F G+QDTL+V+    F++ C+IYGT+DFIFGN   ++Q+  +YAR  N    I  
Sbjct: 340 FYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAF 399

Query: 192 TAQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVV 251
           TAQS+    Q +G +  NC ++A+P+    K  +F+ YLGRPWR YS  + ++SF+D ++
Sbjct: 400 TAQSRNQSDQPTGISILNCRILAAPDLIPVKE-NFKAYLGRPWRKYSRTVIIKSFIDDLI 459

Query: 252 NPAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFING 311
           +PAGW        L  L+Y EY N GPGA++ +RV WPGF  I N  EAT FTV  FI+G
Sbjct: 460 HPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDG 519

Query: 312 YTWLPETGVPF 315
            TWL  TG+PF
Sbjct: 520 STWLNSTGIPF 528

[Close]

BLAST of Tan0017311 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 268.5 bits (685), Expect = 7.0e-72
Identity = 136/311 (43.73%), Postives = 198/311 (63.67%), Query Frame = 0


Query: 16  VSLDGSGNFKSIGAAIAAAPNNSKS---------RFGIYKEQVEVESQKTFIALIGENAA 75
           VS DG+GNF +I AA+AAAPNN+             GIY+E + +   K ++ +IG+   
Sbjct: 267 VSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGIN 326

Query: 76  TTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAVY 135
            TV+  NRS   G+ T  +ATF V   NF+A +ITF+N+AGPE  QAVA L +GA+++++
Sbjct: 327 QTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVA-LRSGADFSIF 386

Query: 136 YKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYAR--LANTTITIT 195
           Y C F  +QDTL+ +    F++ C++YGTVDFIFGN   ++Q+C +Y R  + N    IT
Sbjct: 387 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 446

Query: 196 AQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVN 255
           AQ +   +Q +G + QNC++  + +  S      + YLGRPW++YS  ++M+S++D  V 
Sbjct: 447 AQGRSDPNQNTGTSIQNCTIKPADDLVS-SNYTVKTYLGRPWKEYSRTVYMQSYIDGFVE 506

Query: 256 PAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVK-LFING 315
           P GW  W+    L+ L+YAEYNN GPG++ T RV WPG+HVI N  +A NFTV  LFI  
Sbjct: 507 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI-NSTDAANFTVTGLFIEA 566

[Close]

BLAST of Tan0017311 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 268.1 bits (684), Expect = 9.2e-72
Identity = 134/309 (43.37%), Postives = 194/309 (62.78%), Query Frame = 0


Query: 15  IVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENAATT 74
           +V+ DG+GNF +I  AI+ APN S  R       G+Y E +++   KT I LIG+ +  T
Sbjct: 239 VVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVT 298

Query: 75  VIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAVYYK 134
            I  NRS G G+ T  +AT  V G  F+A+ I   N+AGPE  QAVAL  N A++   Y+
Sbjct: 299 FITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVN-ADFVALYR 358

Query: 135 CVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARL--ANTTITITAQ 194
           CV  G+QDTL+ +    F++ C+IYGT+D+IFGN   ++Q C I ++L        ITAQ
Sbjct: 359 CVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQ 418

Query: 195 SKEFQSQRSGFTFQNCSVMASPE-FGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVNP 254
           S++ Q + +G + QNCS++AS + F S  ++  + YLGRPWR++S  + MES++D  ++ 
Sbjct: 419 SRDTQDEDTGISMQNCSILASEDLFNSSNKV--KSYLGRPWREFSRTVVMESYIDEFIDG 478

Query: 255 AGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFINGYT 314
           +GWS W+ G  L+ L+Y EYNN GPG++  +RV WPGFH++   E+A NFT   FI G  
Sbjct: 479 SGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGY-EDAFNFTATEFITGDG 538

[Close]

BLAST of Tan0017311 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 266.5 bits (680), Expect = 2.7e-71
Identity = 132/310 (42.58%), Postives = 199/310 (64.19%), Query Frame = 0


Query: 13  NAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENAA 72
           N +V+ +G+GN+ +IG AI+AAPN+S++RF      G Y E +E+  +KT I  IG+   
Sbjct: 207 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 266

Query: 73  TTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAVY 132
            TVI  NRS   G+   ++AT  V+G+ F+A+ ++F N AGPE  QAVA L + ++ + Y
Sbjct: 267 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVA-LRSSSDLSAY 326

Query: 133 YKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYARLANTT--ITIT 192
           Y+C F  +QDT++V+    F++ C+IYGTVDFIFG+   ++Q+C +YAR  N    I  T
Sbjct: 327 YRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYT 386

Query: 193 AQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVN 252
           AQ +E   + +G +  +  ++A+P+     + +F+ YLGRPW+ YS  + M+SF+D +V+
Sbjct: 387 AQGRENSREPTGISIISSRILAAPDL-IPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVD 446

Query: 253 PAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFINGY 312
           PAGW  W     L  L+Y EY N GPG+++T RV+WPGF  I   EEA+ F+V  FI+G 
Sbjct: 447 PAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGN 506

Query: 313 TWLPETGVPF 315
            WL  T +PF
Sbjct: 507 KWLNSTRIPF 514

[Close]

BLAST of Tan0017311 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 265.4 bits (677), Expect = 5.9e-71
Identity = 134/310 (43.23%), Postives = 192/310 (61.94%), Query Frame = 0


Query: 13  NAIVSLDGSGNFKSIGAAIAAAPNNSKSRF------GIYKEQVEVESQKTFIALIGENAA 72
           +A+V+ DG+GNF +I  A+ AAP+ S  R+      G+Y E VE++ +K  I ++G+   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 73  TTVIVDNRSNGTGFGTSYTATFVVKGNNFMAQSITFKNSAGPENGQAVALLNNGANYAVY 132
            TVI  NRS   G+ T  +ATF V G  F+A+ ITF+N+AGPE  QAVA + +  +  V+
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVA-IRSDTDLGVF 333

Query: 133 YKCVFLGFQDTLFVNGDNLFFKNCEIYGTVDFIFGNGRALYQDCKIYAR--LANTTITIT 192
           Y+C   G+QDTL+ +    FF+ C I GTVDFIFG+  A++Q C+I A+  L N   +IT
Sbjct: 334 YRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSIT 393

Query: 193 AQSKEFQSQRSGFTFQNCSVMASPEFGSRKRLDFEVYLGRPWRDYSTVIFMESFLDFVVN 252
           AQ ++  ++ +GFT Q  ++ A  +           YLGRPW+ YS  +FM++++   +N
Sbjct: 394 AQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTT-ATYLGRPWKLYSRTVFMQNYMSDAIN 453

Query: 253 PAGWSPWSTGRPLNLLFYAEYNNRGPGADLTRRVKWPGFHVISNPEEATNFTVKLFINGY 312
           P GW  W+    L+ L+Y EY N GPGA L RRVKWPG+HV++   EA NFTV   I G 
Sbjct: 454 PVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGN 513

Query: 313 TWLPETGVPF 315
            WLP TG+ F
Sbjct: 514 LWLPSTGITF 521

The following BLAST results are available for this feature:

BLAST of Tan0017311 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81415	5.6e-74	45.34	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
O04887	3.1e-72	44.34	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
Q9SRX4	9.9e-71	43.73	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
O48711	1.3e-70	43.37	Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3...	[more]
O81301	3.7e-70	42.58	Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Tan0017311 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6584281.1	4.6e-118	67.08	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
KAG6584282.1	1.4e-117	67.38	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
KAG7019877.1	2.9e-112	67.43	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
XP_022137308.1	3.9e-109	63.66	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_022150332.1	5.9e-105	62.62	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]

BLAST of Tan0017311 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C9Z6	1.9e-109	63.66	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008805 PE=4 SV=1	[more]
A0A6J1DAG5	2.9e-105	62.62	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1	[more]
A0A6J1D381	1.4e-104	62.18	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1	[more]
A0A6J1GK92	7.0e-104	62.81	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1	[more]
A0A6J1GM54	7.8e-103	61.30	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1	[more]

BLAST of Tan0017311 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02300.1	4.0e-75	45.34	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	7.0e-72	43.73	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G26440.1	9.2e-72	43.37	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02320.1	2.7e-71	42.58	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G43270.1	5.9e-71	43.23	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 10..318 e-value: 6.8E-106 score: 356.2
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 13..303 e-value: 8.8E-100 score: 333.3
None	No IPR available	PANTHER	PTHR31707:SF205	PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATED	coord: 12..315
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 12..315
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 152..161
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 14..316

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0017311.1	Tan0017311.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
cellular_component	GO:0016020	membrane
molecular_function	GO:0030599	pectinesterase activity