Tan0017032 (gene) Snake gourd v1

BLAST of Tan0017032 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 2.4e-213
Identity = 395/759 (52.04%), Postives = 530/759 (69.83%), Query Frame = 0

Query: 2   NYKLPKIFLTFIFLSAAVASST---HVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAV 61
           N ++    L F  ++ AV ++T   +V R+ Y+VYMG   +    E  A++HH+LL   +
Sbjct: 4   NQRVRLFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEA-AENHHNLLMTVI 63

Query: 62  GDDELARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLG 121
           GD+  AR+ KIYSYG++ NGF ARL PHEA+KL++E+ VVSVF++ +R+LHTTRSWDFLG
Sbjct: 64  GDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLG 123

Query: 122 LSEVASRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRK 181
           L E   +R+  +ES IIVG+LD+GI ++SPSFND G    P+ WKG CV G+NFT CN K
Sbjct: 124 LVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNK 183

Query: 182 VIGARFFNL--ENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG---- 241
           VIGA++F++  E + +    +  D  GHGTHTSST+AG +V  ASL G+A GTARG    
Sbjct: 184 VIGAKYFHIQSEGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPS 243

Query: 242 -------VCWSIGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEK 301
                  VCW  GCTD D+LA FD AI+DGVDIIS+SIGG    FFEDPIAIG+FHAM++
Sbjct: 244 ARIAAYKVCWDSGCTDMDMLAAFDEAISDGVDIISISIGGASLPFFEDPIAIGAFHAMKR 303

Query: 302 GILTSCSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPK 361
           GILT+CSAGN+GP L TV N+APW+MTVAA+++DR F T++KLGN    +G+S+N F+P+
Sbjct: 304 GILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPR 363

Query: 362 KKMYPLISGAKAA-LPEHGSLDPRWCDYGSLDENKVKGKIVYC--------LGSLYQEYT 421
           KKMYPL SG+ A+ L   G  +P  C+ G+L E+KV GK+VYC         G   Q++ 
Sbjct: 364 KKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHV 423

Query: 422 ISDLGGVGVIANLMNLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKT 481
           +  L G GVI  L+  T++A  T I  +++  +    +  YINSTKNPQAVI KT T K 
Sbjct: 424 VRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTKM 483

Query: 482 EAPFLAYFSSRGPQFVAHNILKPDIAAPGMNILAAYSKLATI---PID-RHATFSIMSGT 541
            AP ++ FS+RGPQ ++ NILKPDI+APG+NILAAYSKLA++   P D R   FSIMSGT
Sbjct: 484 LAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGT 543

Query: 542 SMACPHAAAAAAYLKTFHPTWSPAAIKSALMTTATPLKIGERKDELGAGAGQINPTKAIH 601
           SMACPHAAAAAAY+K+FHP WSPAAIKSALMTTATP++I   + EL  G+GQINP +AIH
Sbjct: 544 SMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGNEAELSYGSGQINPRRAIH 603

Query: 602 PGLIFNISHNSYLSFLCSKNHYDGSALAILAGNSS-------FNCSDLTLPSGSDGLNYP 661
           PGL+++I+ ++YL FLC K  Y+ +++ +L G++S       +NC ++    GSDGLNYP
Sbjct: 604 PGLVYDITEDAYLRFLC-KEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYP 663

Query: 662 SMYIPIELGATAVSAIFKRVATHVGFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRS 721
           S++  +      VS +F R  T+VG+GPSTY A+V +P G+ V V+P+ + F +  E R+
Sbjct: 664 SLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRN 723

Query: 722 FKVMVKGTVAAEGSLPMKASLEWNDSK-HNVRIPILLFR 724
           FKV++ G         + AS+EW+DS+ H VR PILLFR
Sbjct: 724 FKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLFR 760

BLAST of Tan0017032 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 2.9e-166
Identity = 332/741 (44.80%), Postives = 465/741 (62.75%), Query Frame = 0

Query: 2   NYKLPKIFLTFIFLSAAVASS-THVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGD 61
           ++ L  + +  +++S   AS+     +  Y++Y+G  P   + E     H +LL++    
Sbjct: 9   HHHLLVLVMVVLWISPRYASAEDEHAKDFYIIYLGDRP--DNTEETIKTHINLLSSLNIS 68

Query: 62  DELARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLS 121
            E A++ K+YSY ++FN FAA+L PHEAKK+ + + VVSV  ++ RKLHTT+SWDF+GL 
Sbjct: 69  QEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGL- 128

Query: 122 EVASRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVI 181
            + ++R+   E  +I+G+LD+GI  DS SF D+G    P+ WKG C    NFTGCN K+I
Sbjct: 129 PLTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKII 188

Query: 182 GARFFNLE-NIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG------- 241
           GA++F  + N+     +SP+D  GHGTHTSSTVAG  V  ASL G+A GTARG       
Sbjct: 189 GAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARL 248

Query: 242 ----VCWS-IGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEKGI 301
               VCW+  GC D D+LAGF+ AI DGV+IIS+SIGG  +++  D I++GSFHAM KGI
Sbjct: 249 AMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGI 308

Query: 302 LTSCSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKK 361
           LT  SAGN GP   TV N  PWI+TVAAS IDR F + + LGN K F+G+ ++ FSPK K
Sbjct: 309 LTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAK 368

Query: 362 MYPLISGAKAALPEHGSLDPRWCDYGSLDENKVKGKIVYC-LGSLYQEYTISDLGGVGVI 421
            YPL+SG  AA         R+C   SLD  KVKGK++ C +G    E TI   GG G I
Sbjct: 369 SYPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAI 428

Query: 422 ANLMNLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKTEAPFLAYFSS 481
                  + A I   P+T ++S   + +  YINST++  AVI KT      APF+A FSS
Sbjct: 429 IVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSS 488

Query: 482 RGPQFVAHNILKPDIAAPGMNILAAYS-KLATIPID---RHATFSIMSGTSMACPHAAAA 541
           RGP   +  +LKPDIAAPG++ILAA++ K +   +D   + + F+I+SGTSMACPH A  
Sbjct: 489 RGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGV 548

Query: 542 AAYLKTFHPTWSPAAIKSALMTTATPLKIGERKD-ELGAGAGQINPTKAIHPGLIFNISH 601
           AAY+K+FHP W+PAAIKSA++T+A P+     KD E   G GQINP +A  PGL++++  
Sbjct: 549 AAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDD 608

Query: 602 NSYLSFLCSKNHYDGSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAI 661
            SY+ FLC +  Y+ + LA L G  S +CS +    G D LNYP++ + +    T+  A+
Sbjct: 609 ISYVQFLCGEG-YNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAV 668

Query: 662 FKRVATHVGFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLP 721
           F+R  T+VG   S Y A V++P GV + V P++L F+++ + RSFKV+VK      G + 
Sbjct: 669 FRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKI- 728

Query: 722 MKASLEWNDSKHNVRIPILLF 723
           +   L W   +H+VR PI+++
Sbjct: 729 VSGLLVWKSPRHSVRSPIVIY 744

BLAST of Tan0017032 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 1.4e-149
Identity = 315/738 (42.68%), Postives = 436/738 (59.08%), Query Frame = 0

Query: 7   KIFLTFIFLSAAVAS---STHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGDDEL 66
           K+F   +F S  +AS   S    +  Y+VYMG   KL+  +    HH ++L   VG    
Sbjct: 8   KLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGR--KLEDPDSAHLHHRAMLEQVVG-STF 67

Query: 67  ARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLSEVA 126
           A +S +++Y RSFNGFA +L   EA+K+A  + VVSVF ++  +LHTTRSWDFLG     
Sbjct: 68  APESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTV 127

Query: 127 SRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVIGAR 186
            RR + VES I+VG+LD+GIW +SPSF+D G+   P  WKG C   +NF  CNRK+IGAR
Sbjct: 128 PRR-SQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFR-CNRKIIGAR 187

Query: 187 FFNL-ENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG---------- 246
            +++   I       P D  GHGTHT+ST AG  V  A+L G+  GTARG          
Sbjct: 188 SYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAY 247

Query: 247 -VCWSIGCTDTDLLAGFDHAIADGVDIISVSIGG-GPSEFFEDPIAIGSFHAMEKGILTS 306
            VCW+ GC+DTD+LA +D AIADGVDIIS+S+GG  P  +F D IAIGSFHA+E+GILTS
Sbjct: 248 KVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTS 307

Query: 307 CSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKKMYP 366
            SAGN GP   T  +++PW+++VAAST+DR F T +++GN + F GVS+NTF    + YP
Sbjct: 308 NSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYP 367

Query: 367 LISGAKAALPEHGSLDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVGVIANLM 426
           L+SG             R+C   S++ N +KGKIV C  S         L G   +    
Sbjct: 368 LVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 427

Query: 427 NLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTT-KKTEAPFLAYFSSRGP 486
           N  + A   P+PS+ L          YI S ++P A I K+TT     AP +  FSSRGP
Sbjct: 428 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 487

Query: 487 QFVAHNILKPDIAAPGMNILAAYSKLATI-PIDRHATFSIMSGTSMACPHAAAAAAYLKT 546
                +++KPDI+ PG+ ILAA+  +A +  I R+  F+I+SGTSM+CPH    A Y+KT
Sbjct: 488 NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT 547

Query: 547 FHPTWSPAAIKSALMTTATPLKIG-ERKDELGAGAGQINPTKAIHPGLIFNISHNSYLSF 606
           ++PTWSPAAIKSALMTTA+P+      + E   G+G +NP KA+ PGL+++ + + Y+ F
Sbjct: 548 YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 607

Query: 607 LCSKNHYDGSALAILAGNSSFNCSDLTLPSGSDG----LNYPSMYIPIELGATAVSAIFK 666
           LC +  Y+  A+  + G+ S         SG+ G    LNYPS  + +    T  +  F 
Sbjct: 608 LCGQG-YNTQAVRRITGDYS------ACTSGNTGRVWDLNYPSFGLSVSPSQT-FNQYFN 667

Query: 667 RVATHVGFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMK 722
           R  T V    STY+A + +P G+++ V P  L FN   + +SF + V+G++       + 
Sbjct: 668 RTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIK---GFVVS 727

BLAST of Tan0017032 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 4.1e-149
Identity = 324/739 (43.84%), Postives = 434/739 (58.73%), Query Frame = 0

Query: 8   IFLTFIFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGDDELARKS 67
           I L FIF     A+        Y+VYMG LP+++       HH S+L   VG    A   
Sbjct: 11  ICLAFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSP--PSHHLSILQKLVG-TIAASHL 70

Query: 68  KIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLSEVASRRN 127
            + SY RSFNGFAA L   E++KL   K VVSVF SK  +L TTRSWDF+G  E A RR 
Sbjct: 71  LVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKA-RRE 130

Query: 128 AAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVIGARFFNL 187
           +  ES +IVG++DSGIW +S SF+D G+   P  WKG C  G  F  CN K+IGARF+  
Sbjct: 131 SVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKF-ACNNKLIGARFY-- 190

Query: 188 ENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG-----------VCWS 247
               N    S  DE GHGTHT+ST AG+ V  AS  G+A+GTARG           VC++
Sbjct: 191 ----NKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN 250

Query: 248 IGCTDTDLLAGFDHAIADGVDIISVSIGGG-PSEFFEDPIAIGSFHAMEKGILTSCSAGN 307
             C D D+LA FD AIADGVD+IS+SI     S      +AIGSFHAM +GI+T+ SAGN
Sbjct: 251 -RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGN 310

Query: 308 SGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKKMYPLISGA 367
           +GP+  +V NV+PW++TVAAS  DR F   + LGN K   G+SVNTF+     +P++ G 
Sbjct: 311 NGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQ 370

Query: 368 KAALPEHGSLDPRWCDYGSLDENKVKGKIVYCLGSL-YQEYTISDLGGVGVIANLMNLTE 427
             +          +C  G +D   VKGKIV C   L Y+E  ++  G +GVI     L +
Sbjct: 371 NVS-RNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLA--GAIGVIVQNTLLPD 430

Query: 428 IAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKT-TTKKTEAPFLAYFSSRGPQFVA 487
            A + P P++ L  +    + +YI S + PQA IL+T      EAP++  FSSRGP FV 
Sbjct: 431 SAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVI 490

Query: 488 HNILKPDIAAPGMNILAAYSKLAT-----IPID-RHATFSIMSGTSMACPHAAAAAAYLK 547
            N+LKPD++APG+ ILAA+S +A+      P D R   +S+MSGTSMACPH A  AAY+K
Sbjct: 491 QNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVK 550

Query: 548 TFHPTWSPAAIKSALMTTATPLKIGER-KDELGAGAGQINPTKAIHPGLIFNISHNSYLS 607
           +FHP WSP+AIKSA+MTTATP+ + +  + E   G+GQINPTKA  PGL++ +    YL 
Sbjct: 551 SFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLK 610

Query: 608 FLCSKNHYDGSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAIFKRVA 667
            LC++  +D + L   +G  +  CS+ T       LNYP+M   +       +  FKR  
Sbjct: 611 MLCAEG-FDSTTLTTTSG-QNVTCSERT---EVKDLNYPTMTTFVS-SLDPFNVTFKRTV 670

Query: 668 THVGFGPSTYKAK-VKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKAS 725
           T+VGF  STYKA  V     + + + PE L+F    E +SF V + G    +GS  + +S
Sbjct: 671 TNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSF-VSSS 727

BLAST of Tan0017032 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 2.8e-145
Identity = 317/733 (43.25%), Postives = 431/733 (58.80%), Query Frame = 0

Query: 13  IFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGDDELARKSKIYSY 72
           + L ++V++      Q Y+VYMG+L     +   +D H S+L    G+  +  +  + SY
Sbjct: 16  VLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSD-HMSILQQVTGESSIEGR-LVRSY 75

Query: 73  GRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLSE-VASRRNAAVE 132
            RSFNGFAARL   E   +A+ + VVSVF +K  +LHTT SWDF+G+ E   ++RN A+E
Sbjct: 76  KRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIE 135

Query: 133 SKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVIGARFFNLENID 192
           S  I+G++D+GIW +S SF+D G+   P  WKG C  G NFT CN K+IGAR +  E   
Sbjct: 136 SDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT-CNNKLIGARDYTSEGTR 195

Query: 193 NTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG-----------VCWSIGCT 252
           +T         GHGTHT+ST AG+ V   S  G+  GT RG           VC   GC+
Sbjct: 196 DT--------SGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCS 255

Query: 253 DTDLLAGFDHAIADGVDIISVSIG-GGPSEFFEDPIAIGSFHAMEKGILTSCSAGNSGPE 312
              LL+ FD AIADGVD+I++SIG   PS F +DPIAIG+FHAM KGILT  SAGNSGP+
Sbjct: 256 SEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPK 315

Query: 313 LRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKKMYPLISGAKAAL 372
             TV +VAPWI TVAAST +R F T + LGN K  AG SVN F  K K YPL+ G  AA 
Sbjct: 316 PTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAAS 375

Query: 373 PEHGSLDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVGVIANLMNLTEIAIIT 432
               +     C    L++++VKGKI+ C G     Y I+   G   I +     ++A   
Sbjct: 376 SACDAKTAALCAPACLNKSRVKGKILVCGGP--SGYKIAKSVGAIAIIDKSPRPDVAFTH 435

Query: 433 PIPSTHLSSQHSNFVDAYINSTKNPQAVILKT-TTKKTEAPFLAYFSSRGPQFVAHNILK 492
            +P++ L ++    + +YI S  +PQA +LKT T     +P +A FSSRGP  +A +ILK
Sbjct: 436 HLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILK 495

Query: 493 PDIAAPGMNILAAYSKLATIPID--RHATFSIMSGTSMACPHAAAAAAYLKTFHPTWSPA 552
           PDI APG+ ILAA+S       D  R   +S+ SGTSMACPH A  AAY+KTF+P WSP+
Sbjct: 496 PDITAPGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPS 555

Query: 553 AIKSALMTTATPLKI---GERKDELGAGAGQINPTKAIHPGLIFNISHNSYLSFLCSKNH 612
            I+SA+MTTA P+K    G    E   GAG ++P  A++PGL++ +    +++FLC  N 
Sbjct: 556 MIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMN- 615

Query: 613 YDGSALAILAGNSSFNCS--DLTLPSGSDGLNYPSMYIPIELGATAVSAIFKRVATHVGF 672
           Y    L I++G+ +  CS  +  LP     LNYPSM   +    +  S  F R  T+VG 
Sbjct: 616 YTSKTLKIISGD-TVKCSKKNKILPR---NLNYPSMSAKLSGTDSTFSVTFNRTLTNVGT 675

Query: 673 GPSTYKAKVKSPAG--VSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKASLEWN 723
             STYK+KV +  G  +S++V P  L F    E +SF V V G+   +  +P  A+L W+
Sbjct: 676 PNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGS-DVDSEVPSSANLIWS 729

BLAST of Tan0017032 vs. NCBI nr
Match: XP_023539892.1 (subtilisin-like protease SBT4.15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 579/734 (78.88%), Postives = 644/734 (87.74%), Query Frame = 0

Query: 1   MNYKLPKIFLTFIFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGD 60
           MNYK+ K FL+FI L AAVASSTHVPR+AYVVYMGALPK+QSH VL  +HHSLLANAVGD
Sbjct: 1   MNYKMLKGFLSFISLIAAVASSTHVPRKAYVVYMGALPKVQSHGVLEQYHHSLLANAVGD 60

Query: 61  DELARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLS 120
           +ELARKSKIYSYGRSFNGFAA+LLPHEA+KLAKEK VVSVF+S  RKLHTTRSWDFLGLS
Sbjct: 61  EELARKSKIYSYGRSFNGFAAKLLPHEAQKLAKEKGVVSVFQSTTRKLHTTRSWDFLGLS 120

Query: 121 EVASRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVI 180
             AS RNAA ES +IVG+LDSGIWM SPSF D+GY +IPS WKG CV GHNFTGCNRKVI
Sbjct: 121 VAASHRNAAAESDMIVGLLDSGIWMGSPSFKDDGYGEIPSKWKGHCVTGHNFTGCNRKVI 180

Query: 181 GARFFNLENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG-------- 240
           GARFFNL+ ID TINQSP DE+GHG+HTSSTVAG+TV GASL G+A GTARG        
Sbjct: 181 GARFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA 240

Query: 241 ---VCWSIGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEKGILT 300
              VCW++GCTD DLLAGFDHAIADGVDIISVSIGGG  EFFEDPIAIGSFHAM KGILT
Sbjct: 241 MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT 300

Query: 301 SCSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKKMY 360
           SCSAGN+GP L TVEN APWIMTVAASTIDRDFST++KLGNNKKF G+S+NTFS KKKM 
Sbjct: 301 SCSAGNNGPGLTTVENSAPWIMTVAASTIDRDFSTVVKLGNNKKFQGISLNTFSTKKKMS 360

Query: 361 PLISGAKAALPEHGSLDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVGVIANL 420
           PLISG +AALP H   + RWCD GSLD+ KVKGKI+YCLGSL QEYTIS+LGGVGVIANL
Sbjct: 361 PLISGGRAALPNHP--NARWCDGGSLDKGKVKGKILYCLGSLDQEYTISELGGVGVIANL 420

Query: 421 MNLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKTEAPFLAYFSSRGP 480
           MN TE+  +TPIP+THLSSQ S+ V+ YINST+ P+AVI+KTTT KTEAPFLAYFSSRGP
Sbjct: 421 MNNTEVLAVTPIPATHLSSQDSDIVETYINSTQKPKAVIMKTTTTKTEAPFLAYFSSRGP 480

Query: 481 QFVAHNILKPDIAAPGMNILAAYSKLATIPIDRHATFSIMSGTSMACPHAAAAAAYLKTF 540
           QF+A +ILKPD+AAPGMNILAA +KLATIP  +H+TF IMSGTSMACPH AAAAAYLKTF
Sbjct: 481 QFIARDILKPDVAAPGMNILAANTKLATIPTGKHSTFDIMSGTSMACPHVAAAAAYLKTF 540

Query: 541 HPTWSPAAIKSALMTTATPLKIGERKDELGAGAGQINPTKAIHPGLIFNISHNSYLSFLC 600
           HPTWSPAAIKSALMTTATPLKIGE +DELGAGAGQINPTKA++PGLIF++S  SY+SFLC
Sbjct: 541 HPTWSPAAIKSALMTTATPLKIGESQDELGAGAGQINPTKAVNPGLIFDLSRTSYISFLC 600

Query: 601 SKNHYDGSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAIFKRVATHV 660
           +KNHY G+ALAIL G+SSFNCS +T  +GSDGLNYPSMY+PI+L ATAVSA+F R+ THV
Sbjct: 601 NKNHYAGTALAILTGDSSFNCSAVTPNTGSDGLNYPSMYVPIDLQATAVSAVFHRIVTHV 660

Query: 661 GFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKASLEWN 720
           G GPSTYKAKVKSP G+SVRVLPE LKF ++ ETRSFKVMVKG VAAEG++ +KASLEWN
Sbjct: 661 GSGPSTYKAKVKSPMGLSVRVLPEALKFKRANETRSFKVMVKGAVAAEGNVLLKASLEWN 720

Query: 721 DSKHNVRIPILLFR 724
           DSKHNVRIPIL FR
Sbjct: 721 DSKHNVRIPILAFR 732

BLAST of Tan0017032 vs. NCBI nr
Match: XP_022950578.1 (subtilisin-like protease SBT4.15 [Cucurbita moschata])

HSP 1 Score: 1151.7 bits (2978), Expect = 0.0e+00
Identity = 575/734 (78.34%), Postives = 646/734 (88.01%), Query Frame = 0

Query: 1   MNYKLPKIFLTFIFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGD 60
           MNYK+ K FL+FI L AAVASST++PR+AYVVYMGALPK+QSH VL ++HHSLLANAVGD
Sbjct: 1   MNYKMLKGFLSFISLIAAVASSTNIPRKAYVVYMGALPKVQSHGVLEEYHHSLLANAVGD 60

Query: 61  DELARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLS 120
           +ELARKSKIYSYGRSFNGFAA+LLPHEA+KLAKEKSVVSVF S  RKLHTTRSWDFLGLS
Sbjct: 61  EELARKSKIYSYGRSFNGFAAKLLPHEAQKLAKEKSVVSVFRSTIRKLHTTRSWDFLGLS 120

Query: 121 EVASRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVI 180
             ASRRNAA ES +IVG+LDSGIWM SPSF D+GY +IPS WKG CV GHNFTGCNRKVI
Sbjct: 121 AAASRRNAAAESDMIVGLLDSGIWMGSPSFKDDGYGEIPSKWKGHCVTGHNFTGCNRKVI 180

Query: 181 GARFFNLENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG-------- 240
           GARFFNL+ ID TINQSP DE+GHG+HTSSTVAG+TV GASL G+A GTARG        
Sbjct: 181 GARFFNLQEIDATINQSPADEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA 240

Query: 241 ---VCWSIGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEKGILT 300
              VCW++GCTD DLLAGFDHAIADGVDIISVSIGGG  EFFEDPIAIGSFHAM KGILT
Sbjct: 241 MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT 300

Query: 301 SCSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKKMY 360
           SCSAGN+GP L TVEN APWIMTVAASTIDRDFST++KLGNNKK +G+S+NTFS KKKMY
Sbjct: 301 SCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGISLNTFSTKKKMY 360

Query: 361 PLISGAKAALPEHGSLDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVGVIANL 420
           PLISG  AALP H   + RWCD GSLD+ KV+GKI+YCLGSL QEYT+S+LGG GVIANL
Sbjct: 361 PLISGGSAALPNHP--NARWCDGGSLDKGKVEGKILYCLGSLDQEYTVSELGGAGVIANL 420

Query: 421 MNLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKTEAPFLAYFSSRGP 480
           MN TE+  +TPIP+THLSSQ S+ V+AYIN+T+ P+AVI+KTTT KTEAPFLAYFSSRGP
Sbjct: 421 MNNTEVLAVTPIPATHLSSQDSDIVEAYINATQKPKAVIMKTTTTKTEAPFLAYFSSRGP 480

Query: 481 QFVAHNILKPDIAAPGMNILAAYSKLATIPIDRHATFSIMSGTSMACPHAAAAAAYLKTF 540
           QF+A +ILKPD+AAPGMNILAA +KLATIP  +H+TF IMSGTSMACPH AAAAAYLKTF
Sbjct: 481 QFIARDILKPDVAAPGMNILAANTKLATIPTGKHSTFDIMSGTSMACPHVAAAAAYLKTF 540

Query: 541 HPTWSPAAIKSALMTTATPLKIGERKDELGAGAGQINPTKAIHPGLIFNISHNSYLSFLC 600
           HPTWSPAAIKSALMTTATPLKIGE +DELG GAGQINPTKA++PGLIF++S  SY+SFLC
Sbjct: 541 HPTWSPAAIKSALMTTATPLKIGESQDELGVGAGQINPTKAVNPGLIFDLSRTSYISFLC 600

Query: 601 SKNHYDGSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAIFKRVATHV 660
           +KNHY G+ALAILAG+SSFNCS +T  +GSDGLNYPSMY+P++L ATAVSA+F R+ THV
Sbjct: 601 NKNHYAGTALAILAGDSSFNCSAVTPNTGSDGLNYPSMYVPVDLQATAVSAVFHRIVTHV 660

Query: 661 GFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKASLEWN 720
           G GPSTYKAKVKSP G+SVRVLPE LKF ++ ETRSFKVMVKG VAAEG++ +KASLEWN
Sbjct: 661 GSGPSTYKAKVKSPMGLSVRVLPEALKFKRANETRSFKVMVKGAVAAEGNVLLKASLEWN 720

Query: 721 DSKHNVRIPILLFR 724
           DSKHNVRIPIL FR
Sbjct: 721 DSKHNVRIPILAFR 732

BLAST of Tan0017032 vs. NCBI nr
Match: KAG7028095.1 (Subtilisin-like protease SBT4.15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1145.2 bits (2961), Expect = 0.0e+00
Identity = 571/728 (78.43%), Postives = 642/728 (88.19%), Query Frame = 0

Query: 7   KIFLTFIFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGDDELARK 66
           K FL+FI L AAVASST++PR+AYVVYMGALPK+QSH VL ++HHSLLANAVGD+ELARK
Sbjct: 3   KGFLSFISLIAAVASSTNIPRKAYVVYMGALPKVQSHGVLEEYHHSLLANAVGDEELARK 62

Query: 67  SKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLSEVASRR 126
           SKIYSYGRSFNGFAA+LLPHEA+KLAKEK VVSVF+S  RKLHTTRSWDFLGLS  ASRR
Sbjct: 63  SKIYSYGRSFNGFAAKLLPHEAQKLAKEKGVVSVFQSTTRKLHTTRSWDFLGLSTAASRR 122

Query: 127 NAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVIGARFFN 186
           NAA ES +IVG+LDSGIWM SPSF D+GY +IPS WKG CV GHNFTGCNRKVIGARFFN
Sbjct: 123 NAAAESDMIVGLLDSGIWMGSPSFKDDGYGEIPSKWKGHCVTGHNFTGCNRKVIGARFFN 182

Query: 187 LENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG-----------VCW 246
           L+ ID TINQSP DE+GHG+HTSSTVAG+TV GASL G+A GTARG           VCW
Sbjct: 183 LQEIDATINQSPADEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCW 242

Query: 247 SIGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEKGILTSCSAGN 306
           ++GCTD DLLAGFDHAIADGVDIISVSIGGG  EFFEDPIAIGSFHAM KGILTSCSAGN
Sbjct: 243 NVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSAGN 302

Query: 307 SGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKKMYPLISGA 366
           +GP L TVEN APWIMTVAASTIDRDFST++KLGNNKK +G+S+NTFS KKKMYPLISG 
Sbjct: 303 NGPGLTTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGISLNTFSTKKKMYPLISGG 362

Query: 367 KAALPEHGSLDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVGVIANLMNLTEI 426
            AALP H   + RWCD GSLD+ KV+GKI+YCLGSL QEYT+S+LGGVGVIANLMN TE+
Sbjct: 363 SAALPNHP--NARWCDGGSLDKGKVEGKILYCLGSLDQEYTVSELGGVGVIANLMNSTEV 422

Query: 427 AIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKTEAPFLAYFSSRGPQFVAHN 486
             +TPIP+THLSSQ S+ V+AYIN+T+ P+AVI+KTTT KTEAPFLAYFSSRGPQF+A +
Sbjct: 423 LAVTPIPATHLSSQDSDIVEAYINATQKPKAVIMKTTTTKTEAPFLAYFSSRGPQFIARD 482

Query: 487 ILKPDIAAPGMNILAAYSKLATIPIDRHATFSIMSGTSMACPHAAAAAAYLKTFHPTWSP 546
           ILKPD+AAPGMNILAA +KLATIP  +H+TF IMSGTSMACPH AAAAAYLKTFHPTWSP
Sbjct: 483 ILKPDVAAPGMNILAANTKLATIPTGKHSTFDIMSGTSMACPHVAAAAAYLKTFHPTWSP 542

Query: 547 AAIKSALMTTATPLKIGERKDELGAGAGQINPTKAIHPGLIFNISHNSYLSFLCSKNHYD 606
           AAIKSALMTTATPLKIGE +DELG GAGQINPTKA++PGLIF++S  SY+SFLC+KNHY 
Sbjct: 543 AAIKSALMTTATPLKIGESQDELGVGAGQINPTKAVNPGLIFDLSRTSYISFLCNKNHYA 602

Query: 607 GSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAIFKRVATHVGFGPST 666
           G+ALAILAG+SSFNCS +T  +GSDGLNYPSMY+P++L ATAVSA+F R+ THVG GPST
Sbjct: 603 GTALAILAGDSSFNCSAVTPNTGSDGLNYPSMYVPVDLQATAVSAVFHRIVTHVGSGPST 662

Query: 667 YKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKASLEWNDSKHNV 724
           YKAKVKSP G+SVRVLPE LKF ++ ETRSFKVMVKG VAAEG++ +KASLEWNDSKHNV
Sbjct: 663 YKAKVKSPMGLSVRVLPEALKFKRANETRSFKVMVKGAVAAEGNVLLKASLEWNDSKHNV 722

BLAST of Tan0017032 vs. NCBI nr
Match: KAG6596559.1 (Subtilisin-like protease 4.15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 572/728 (78.57%), Postives = 642/728 (88.19%), Query Frame = 0

Query: 7   KIFLTFIFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGDDELARK 66
           K FL+FI L AAVASST++PR+AYVVYMGALPK+QSH VL ++HHSLLANAVGD+ELARK
Sbjct: 3   KGFLSFISLIAAVASSTNIPRKAYVVYMGALPKVQSHGVLEEYHHSLLANAVGDEELARK 62

Query: 67  SKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLSEVASRR 126
           SKIYSYGRSFNGFAA+LLPHEA+KLAKEKSVVSVF S  RKLHTTRSWDFLGLS  ASRR
Sbjct: 63  SKIYSYGRSFNGFAAKLLPHEAQKLAKEKSVVSVFRSTIRKLHTTRSWDFLGLSAAASRR 122

Query: 127 NAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVIGARFFN 186
           N A ES +IVG+LDSGIWM SPSF D+GY +IPS WKG CV GHNFTGCNRKVIGARFFN
Sbjct: 123 NTAAESDMIVGLLDSGIWMGSPSFKDDGYGEIPSKWKGHCVTGHNFTGCNRKVIGARFFN 182

Query: 187 LENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG-----------VCW 246
           L+ ID TINQSP DE+GHG+HTSSTVAG+TV GASL G+A GTARG           VCW
Sbjct: 183 LQEIDATINQSPADEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCW 242

Query: 247 SIGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEKGILTSCSAGN 306
           ++GCTD DLLAGFDHAIADGVDIISVSIGGG  EFFEDPIAIGSFHAM KGILTSCSAGN
Sbjct: 243 NVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSAGN 302

Query: 307 SGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKKMYPLISGA 366
           +GP LRTVEN APWIMTVAASTIDRDFST++KLGNNKK +G+S+NTFS KKKMYPLISG 
Sbjct: 303 NGPGLRTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGISLNTFSTKKKMYPLISGG 362

Query: 367 KAALPEHGSLDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVGVIANLMNLTEI 426
            AALP H   + RWCD GSLD+ KV+GKI+YCLGSL QEYT+S+LGGVGVIANLMN TE+
Sbjct: 363 SAALPNHP--NARWCDGGSLDKVKVEGKILYCLGSLDQEYTVSELGGVGVIANLMNSTEV 422

Query: 427 AIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKTEAPFLAYFSSRGPQFVAHN 486
             +TPIP+THLSSQ S+ V+AYIN+T+ P+AVI+KTTT KTEAPFLAYFSSRGPQF+A +
Sbjct: 423 LAVTPIPATHLSSQDSDIVEAYINATQKPKAVIMKTTTTKTEAPFLAYFSSRGPQFIARD 482

Query: 487 ILKPDIAAPGMNILAAYSKLATIPIDRHATFSIMSGTSMACPHAAAAAAYLKTFHPTWSP 546
           ILKPD+AAPGMNILAA +KLATIP  +H+TF IMSGTSMACPH AAAAAYLKTFHPTWSP
Sbjct: 483 ILKPDVAAPGMNILAANTKLATIPTGKHSTFDIMSGTSMACPHVAAAAAYLKTFHPTWSP 542

Query: 547 AAIKSALMTTATPLKIGERKDELGAGAGQINPTKAIHPGLIFNISHNSYLSFLCSKNHYD 606
           AAIKSALMTTATPLKIGE +DELG GAGQINPTKA++PGLIF++S  SY+SFLC+KNHY 
Sbjct: 543 AAIKSALMTTATPLKIGESQDELGVGAGQINPTKAVNPGLIFDLSRTSYISFLCNKNHYA 602

Query: 607 GSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAIFKRVATHVGFGPST 666
           G+ALAILAG+SSFNCS +T  +GSDGLNYPSMY+P++L ATAVSA+F R+ THVG GPST
Sbjct: 603 GTALAILAGDSSFNCSAVTPNTGSDGLNYPSMYVPVDLQATAVSAVFHRIVTHVGSGPST 662

Query: 667 YKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKASLEWNDSKHNV 724
           YKAKVKSP G+SVRVLPE LKF ++ ETRSFKVMVKG VAAEG++ +KASLEWNDSKHNV
Sbjct: 663 YKAKVKSPMGLSVRVLPEALKFKRANETRSFKVMVKGAVAAEGNVLLKASLEWNDSKHNV 722

BLAST of Tan0017032 vs. NCBI nr
Match: KAA0043897.1 (subtilisin-like protease SBT4.14 [Cucumis melo var. makuwa])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 547/740 (73.92%), Postives = 632/740 (85.41%), Query Frame = 0

Query: 1   MNYKLPK--IFLTFIFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAV 60
           MNY++ K  + LT IFL AA  S+T+  RQAYVVYMGALPKL+S EVLADHHH LLANAV
Sbjct: 1   MNYQMLKLPLILTIIFLFAAAVSATNADRQAYVVYMGALPKLKSPEVLADHHHGLLANAV 60

Query: 61  GDDELARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLG 120
           GD+E+ARK+KIYSYGRSFNGFAARLLPHEA KLA    +VSVF SK RKLHTTRSWDFLG
Sbjct: 61  GDEEMARKAKIYSYGRSFNGFAARLLPHEADKLA----MVSVFRSKTRKLHTTRSWDFLG 120

Query: 121 LSEVASRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRK 180
           LSE ASRRNAA ES +IVG+LDSGIWM+ PSF D+GY +IPS WKG CV GHNFT CNRK
Sbjct: 121 LSEAASRRNAAAESNVIVGLLDSGIWMEGPSFKDDGYGEIPSKWKGKCVTGHNFTSCNRK 180

Query: 181 VIGARFFNLENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG------ 240
           VIGARFF++E IDN+ N+SPVDE+GHG+HT+ST+AG+ V GASL GVA G ARG      
Sbjct: 181 VIGARFFDIEEIDNSNNKSPVDEIGHGSHTASTIAGAFVDGASLYGVAGGKARGGVPAAR 240

Query: 241 -----VCWSIGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEKGI 300
                VCW++GC+D DLLAGFDHAIADGVDIISVSIGG   EFF DPIAIGSFHAMEKGI
Sbjct: 241 IAMYKVCWAVGCSDVDLLAGFDHAIADGVDIISVSIGGESMEFFNDPIAIGSFHAMEKGI 300

Query: 301 LTSCSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKK 360
           LTSCSAGNSGP+L+TVEN APWIMTVAASTIDRDFST++KLGNNKK +GVSVNTF+PKK+
Sbjct: 301 LTSCSAGNSGPDLKTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGVSVNTFTPKKQ 360

Query: 361 MYPLISGAKAALPEHGS---LDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVG 420
           MYPLISG+ AALP   S   L P WCDYGSLDE KVKGKIVYCLGS+ QEYTISDLGGVG
Sbjct: 361 MYPLISGSNAALPNQSSDLDLYPSWCDYGSLDEKKVKGKIVYCLGSIDQEYTISDLGGVG 420

Query: 421 VIANLMNLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKTEAPFLAYF 480
           VI+NL+N+TE+AI TPIPSTHLSS +S++V+AYINSTKNP+AVI KTTT+K +APFLAYF
Sbjct: 421 VISNLLNITEMAITTPIPSTHLSSTNSDYVEAYINSTKNPKAVIYKTTTRKVDAPFLAYF 480

Query: 481 SSRGPQFVAHNILKPDIAAPGMNILAAYSKLATIPIDRHATFSIMSGTSMACPHAAAAAA 540
           SS+GPQ +A NILKPDIAAPG+NILAAYS LA+IP +RH+ F+++SGTSMACPHAAAAAA
Sbjct: 481 SSKGPQTIALNILKPDIAAPGVNILAAYSNLASIPDNRHSLFNLLSGTSMACPHAAAAAA 540

Query: 541 YLKTFHPTWSPAAIKSALMTTATPLKIGERKDELGAGAGQINPTKAIHPGLIFNISHNSY 600
           YLK FHPTWSPAA+KSALMTTATPLKIG+  D +GAGAGQINPTKA+HPGLI+++S  SY
Sbjct: 541 YLKAFHPTWSPAALKSALMTTATPLKIGDELDVIGAGAGQINPTKAVHPGLIYDLSRTSY 600

Query: 601 LSFLCSKNHYDGSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAIFKR 660
           LSFLC+   Y  SALAIL G++S NCSD+   SGSD +NYPSMY+P++  AT+VSA+F R
Sbjct: 601 LSFLCTNKRYSDSALAILTGDASLNCSDVPQASGSDAINYPSMYVPVDRDATSVSAVFHR 660

Query: 661 VATHVGFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKA 720
             THVGFGPSTYKAK+KSPAG+SV+V PETLKF+++Y+  SFKV+VKG   A G  P+ A
Sbjct: 661 TVTHVGFGPSTYKAKIKSPAGLSVKVSPETLKFDRAYKKLSFKVVVKGAAPAVGQAPLTA 720

Query: 721 SLEWNDSKHNVRIPILLFRV 725
           SLEW+DSKH VR PIL+F+V
Sbjct: 721 SLEWDDSKHYVRSPILVFKV 736

BLAST of Tan0017032 vs. ExPASy TrEMBL
Match: A0A6J1GF98 (subtilisin-like protease SBT4.15 OS=Cucurbita moschata OX=3662 GN=LOC111453633 PE=3 SV=1)

HSP 1 Score: 1151.7 bits (2978), Expect = 0.0e+00
Identity = 575/734 (78.34%), Postives = 646/734 (88.01%), Query Frame = 0

Query: 1   MNYKLPKIFLTFIFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGD 60
           MNYK+ K FL+FI L AAVASST++PR+AYVVYMGALPK+QSH VL ++HHSLLANAVGD
Sbjct: 1   MNYKMLKGFLSFISLIAAVASSTNIPRKAYVVYMGALPKVQSHGVLEEYHHSLLANAVGD 60

Query: 61  DELARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLS 120
           +ELARKSKIYSYGRSFNGFAA+LLPHEA+KLAKEKSVVSVF S  RKLHTTRSWDFLGLS
Sbjct: 61  EELARKSKIYSYGRSFNGFAAKLLPHEAQKLAKEKSVVSVFRSTIRKLHTTRSWDFLGLS 120

Query: 121 EVASRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVI 180
             ASRRNAA ES +IVG+LDSGIWM SPSF D+GY +IPS WKG CV GHNFTGCNRKVI
Sbjct: 121 AAASRRNAAAESDMIVGLLDSGIWMGSPSFKDDGYGEIPSKWKGHCVTGHNFTGCNRKVI 180

Query: 181 GARFFNLENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG-------- 240
           GARFFNL+ ID TINQSP DE+GHG+HTSSTVAG+TV GASL G+A GTARG        
Sbjct: 181 GARFFNLQEIDATINQSPADEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA 240

Query: 241 ---VCWSIGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEKGILT 300
              VCW++GCTD DLLAGFDHAIADGVDIISVSIGGG  EFFEDPIAIGSFHAM KGILT
Sbjct: 241 MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT 300

Query: 301 SCSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKKMY 360
           SCSAGN+GP L TVEN APWIMTVAASTIDRDFST++KLGNNKK +G+S+NTFS KKKMY
Sbjct: 301 SCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGISLNTFSTKKKMY 360

Query: 361 PLISGAKAALPEHGSLDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVGVIANL 420
           PLISG  AALP H   + RWCD GSLD+ KV+GKI+YCLGSL QEYT+S+LGG GVIANL
Sbjct: 361 PLISGGSAALPNHP--NARWCDGGSLDKGKVEGKILYCLGSLDQEYTVSELGGAGVIANL 420

Query: 421 MNLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKTEAPFLAYFSSRGP 480
           MN TE+  +TPIP+THLSSQ S+ V+AYIN+T+ P+AVI+KTTT KTEAPFLAYFSSRGP
Sbjct: 421 MNNTEVLAVTPIPATHLSSQDSDIVEAYINATQKPKAVIMKTTTTKTEAPFLAYFSSRGP 480

Query: 481 QFVAHNILKPDIAAPGMNILAAYSKLATIPIDRHATFSIMSGTSMACPHAAAAAAYLKTF 540
           QF+A +ILKPD+AAPGMNILAA +KLATIP  +H+TF IMSGTSMACPH AAAAAYLKTF
Sbjct: 481 QFIARDILKPDVAAPGMNILAANTKLATIPTGKHSTFDIMSGTSMACPHVAAAAAYLKTF 540

Query: 541 HPTWSPAAIKSALMTTATPLKIGERKDELGAGAGQINPTKAIHPGLIFNISHNSYLSFLC 600
           HPTWSPAAIKSALMTTATPLKIGE +DELG GAGQINPTKA++PGLIF++S  SY+SFLC
Sbjct: 541 HPTWSPAAIKSALMTTATPLKIGESQDELGVGAGQINPTKAVNPGLIFDLSRTSYISFLC 600

Query: 601 SKNHYDGSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAIFKRVATHV 660
           +KNHY G+ALAILAG+SSFNCS +T  +GSDGLNYPSMY+P++L ATAVSA+F R+ THV
Sbjct: 601 NKNHYAGTALAILAGDSSFNCSAVTPNTGSDGLNYPSMYVPVDLQATAVSAVFHRIVTHV 660

Query: 661 GFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKASLEWN 720
           G GPSTYKAKVKSP G+SVRVLPE LKF ++ ETRSFKVMVKG VAAEG++ +KASLEWN
Sbjct: 661 GSGPSTYKAKVKSPMGLSVRVLPEALKFKRANETRSFKVMVKGAVAAEGNVLLKASLEWN 720

Query: 721 DSKHNVRIPILLFR 724
           DSKHNVRIPIL FR
Sbjct: 721 DSKHNVRIPILAFR 732

BLAST of Tan0017032 vs. ExPASy TrEMBL
Match: A0A5A7TQ04 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G002370 PE=3 SV=1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 547/740 (73.92%), Postives = 632/740 (85.41%), Query Frame = 0

Query: 1   MNYKLPK--IFLTFIFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAV 60
           MNY++ K  + LT IFL AA  S+T+  RQAYVVYMGALPKL+S EVLADHHH LLANAV
Sbjct: 1   MNYQMLKLPLILTIIFLFAAAVSATNADRQAYVVYMGALPKLKSPEVLADHHHGLLANAV 60

Query: 61  GDDELARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLG 120
           GD+E+ARK+KIYSYGRSFNGFAARLLPHEA KLA    +VSVF SK RKLHTTRSWDFLG
Sbjct: 61  GDEEMARKAKIYSYGRSFNGFAARLLPHEADKLA----MVSVFRSKTRKLHTTRSWDFLG 120

Query: 121 LSEVASRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRK 180
           LSE ASRRNAA ES +IVG+LDSGIWM+ PSF D+GY +IPS WKG CV GHNFT CNRK
Sbjct: 121 LSEAASRRNAAAESNVIVGLLDSGIWMEGPSFKDDGYGEIPSKWKGKCVTGHNFTSCNRK 180

Query: 181 VIGARFFNLENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG------ 240
           VIGARFF++E IDN+ N+SPVDE+GHG+HT+ST+AG+ V GASL GVA G ARG      
Sbjct: 181 VIGARFFDIEEIDNSNNKSPVDEIGHGSHTASTIAGAFVDGASLYGVAGGKARGGVPAAR 240

Query: 241 -----VCWSIGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEKGI 300
                VCW++GC+D DLLAGFDHAIADGVDIISVSIGG   EFF DPIAIGSFHAMEKGI
Sbjct: 241 IAMYKVCWAVGCSDVDLLAGFDHAIADGVDIISVSIGGESMEFFNDPIAIGSFHAMEKGI 300

Query: 301 LTSCSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKK 360
           LTSCSAGNSGP+L+TVEN APWIMTVAASTIDRDFST++KLGNNKK +GVSVNTF+PKK+
Sbjct: 301 LTSCSAGNSGPDLKTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGVSVNTFTPKKQ 360

Query: 361 MYPLISGAKAALPEHGS---LDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVG 420
           MYPLISG+ AALP   S   L P WCDYGSLDE KVKGKIVYCLGS+ QEYTISDLGGVG
Sbjct: 361 MYPLISGSNAALPNQSSDLDLYPSWCDYGSLDEKKVKGKIVYCLGSIDQEYTISDLGGVG 420

Query: 421 VIANLMNLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKTEAPFLAYF 480
           VI+NL+N+TE+AI TPIPSTHLSS +S++V+AYINSTKNP+AVI KTTT+K +APFLAYF
Sbjct: 421 VISNLLNITEMAITTPIPSTHLSSTNSDYVEAYINSTKNPKAVIYKTTTRKVDAPFLAYF 480

Query: 481 SSRGPQFVAHNILKPDIAAPGMNILAAYSKLATIPIDRHATFSIMSGTSMACPHAAAAAA 540
           SS+GPQ +A NILKPDIAAPG+NILAAYS LA+IP +RH+ F+++SGTSMACPHAAAAAA
Sbjct: 481 SSKGPQTIALNILKPDIAAPGVNILAAYSNLASIPDNRHSLFNLLSGTSMACPHAAAAAA 540

Query: 541 YLKTFHPTWSPAAIKSALMTTATPLKIGERKDELGAGAGQINPTKAIHPGLIFNISHNSY 600
           YLK FHPTWSPAA+KSALMTTATPLKIG+  D +GAGAGQINPTKA+HPGLI+++S  SY
Sbjct: 541 YLKAFHPTWSPAALKSALMTTATPLKIGDELDVIGAGAGQINPTKAVHPGLIYDLSRTSY 600

Query: 601 LSFLCSKNHYDGSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAIFKR 660
           LSFLC+   Y  SALAIL G++S NCSD+   SGSD +NYPSMY+P++  AT+VSA+F R
Sbjct: 601 LSFLCTNKRYSDSALAILTGDASLNCSDVPQASGSDAINYPSMYVPVDRDATSVSAVFHR 660

Query: 661 VATHVGFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKA 720
             THVGFGPSTYKAK+KSPAG+SV+V PETLKF+++Y+  SFKV+VKG   A G  P+ A
Sbjct: 661 TVTHVGFGPSTYKAKIKSPAGLSVKVSPETLKFDRAYKKLSFKVVVKGAAPAVGQAPLTA 720

Query: 721 SLEWNDSKHNVRIPILLFRV 725
           SLEW+DSKH VR PIL+F+V
Sbjct: 721 SLEWDDSKHYVRSPILVFKV 736

BLAST of Tan0017032 vs. ExPASy TrEMBL
Match: A0A5D3DPI9 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003690 PE=3 SV=1)

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 546/740 (73.78%), Postives = 631/740 (85.27%), Query Frame = 0

Query: 1   MNYKLPK--IFLTFIFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAV 60
           MNY++ K  + LT IFL AA  S+T+  RQAYVVYMGALPKL+S EVLADHHH LLANAV
Sbjct: 1   MNYQMLKLPLILTIIFLFAAAVSATNADRQAYVVYMGALPKLKSPEVLADHHHGLLANAV 60

Query: 61  GDDELARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLG 120
           GD+E+ARK+KIYSYGRSFNGFAARLLPHE  KLA    +VSVF SK RKLHTTRSWDFLG
Sbjct: 61  GDEEMARKAKIYSYGRSFNGFAARLLPHEVDKLA----MVSVFRSKTRKLHTTRSWDFLG 120

Query: 121 LSEVASRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRK 180
           LSE ASRRNAA ES +IVG+LDSGIWM+ PSF D+GY +IPS WKG CV GHNFT CNRK
Sbjct: 121 LSEAASRRNAAAESNVIVGLLDSGIWMEGPSFKDDGYGEIPSKWKGKCVTGHNFTSCNRK 180

Query: 181 VIGARFFNLENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG------ 240
           VIGARFF++E IDN+ N+SPVDE+GHG+HT+ST+AG+ V GASL GVA G ARG      
Sbjct: 181 VIGARFFDIEEIDNSNNKSPVDEIGHGSHTASTIAGAFVDGASLYGVAGGKARGGVPAAR 240

Query: 241 -----VCWSIGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEKGI 300
                VCW++GC+D DLLAGFDHAIADGVDIISVSIGG   EFF DPIAIGSFHAMEKGI
Sbjct: 241 IAMYKVCWAVGCSDVDLLAGFDHAIADGVDIISVSIGGESMEFFNDPIAIGSFHAMEKGI 300

Query: 301 LTSCSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKK 360
           LTSCSAGNSGP+L+TVEN APWIMTVAASTIDRDFST++KLGNNKK +GVSVNTF+PKK+
Sbjct: 301 LTSCSAGNSGPDLKTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGVSVNTFTPKKQ 360

Query: 361 MYPLISGAKAALPEHGS---LDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVG 420
           MYPLISG+ AALP   S   L P WCDYGSLDE KVKGKIVYCLGS+ QEYTISDLGGVG
Sbjct: 361 MYPLISGSNAALPNQSSDLDLYPSWCDYGSLDEKKVKGKIVYCLGSIDQEYTISDLGGVG 420

Query: 421 VIANLMNLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKTEAPFLAYF 480
           VI+NL+N+TE+AI TPIPSTHLSS +S++V+AYINSTKNP+AVI KTTT+K +APFLAYF
Sbjct: 421 VISNLLNITEMAITTPIPSTHLSSTNSDYVEAYINSTKNPKAVIYKTTTRKVDAPFLAYF 480

Query: 481 SSRGPQFVAHNILKPDIAAPGMNILAAYSKLATIPIDRHATFSIMSGTSMACPHAAAAAA 540
           SS+GPQ +A NILKPDIAAPG+NILAAYS LA+IP +RH+ F+++SGTSMACPHAAAAAA
Sbjct: 481 SSKGPQTIALNILKPDIAAPGVNILAAYSNLASIPDNRHSLFNLLSGTSMACPHAAAAAA 540

Query: 541 YLKTFHPTWSPAAIKSALMTTATPLKIGERKDELGAGAGQINPTKAIHPGLIFNISHNSY 600
           YLK FHPTWSPAA+KSALMTTATPLKIG+  D +GAGAGQINPTKA+HPGLI+++S  SY
Sbjct: 541 YLKAFHPTWSPAALKSALMTTATPLKIGDELDVIGAGAGQINPTKAVHPGLIYDLSRTSY 600

Query: 601 LSFLCSKNHYDGSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAIFKR 660
           LSFLC+   Y  SALAIL G++S NCSD+   SGSD +NYPSMY+P++  AT+VSA+F R
Sbjct: 601 LSFLCTNKRYSDSALAILTGDASLNCSDVPQASGSDAINYPSMYVPVDRDATSVSAVFHR 660

Query: 661 VATHVGFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKA 720
             THVGFGPSTYKAK+KSPAG+SV+V PETLKF+++Y+  SFKV+VKG   A G  P+ A
Sbjct: 661 TVTHVGFGPSTYKAKIKSPAGLSVKVSPETLKFDRAYKKLSFKVVVKGAAPAVGQAPLTA 720

Query: 721 SLEWNDSKHNVRIPILLFRV 725
           SLEW+DSKH VR PIL+F+V
Sbjct: 721 SLEWDDSKHYVRSPILVFKV 736

BLAST of Tan0017032 vs. ExPASy TrEMBL
Match: A0A1S3B6M5 (subtilisin-like protease SBT4.15 OS=Cucumis melo OX=3656 GN=LOC103486592 PE=3 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 4.7e-281
Identity = 479/647 (74.03%), Postives = 555/647 (85.78%), Query Frame = 0

Query: 92  AKEKSVVSVFESKRRKLHTTRSWDFLGLSEVASRRNAAVESKIIVGMLDSGIWMDSPSFN 151
           A+EK VVSVF SK RKLHTTRSWDFLGLSE ASRRNAA ES +IVG+LDSGIWM+ PSF 
Sbjct: 13  AEEKKVVSVFRSKTRKLHTTRSWDFLGLSEAASRRNAAAESNVIVGLLDSGIWMEGPSFK 72

Query: 152 DNGYPQIPSNWKGDCVIGHNFTGCNRKVIGARFFNLENIDNTINQSPVDEMGHGTHTSST 211
           D+GY +IPS WKG CV GHNFT CNRKVIGARFF++E IDN+ N+SPVDE+GHG+HT+ST
Sbjct: 73  DDGYGEIPSKWKGKCVTGHNFTSCNRKVIGARFFDIEEIDNSNNKSPVDEIGHGSHTAST 132

Query: 212 VAGSTVYGASLDGVAEGTARG-----------VCWSIGCTDTDLLAGFDHAIADGVDIIS 271
           +AG+ V GASL GVA G ARG           VCW++GC+D DLLAGFDHAIADGVDIIS
Sbjct: 133 IAGAFVDGASLYGVAGGKARGGVPAARIAMYKVCWAVGCSDVDLLAGFDHAIADGVDIIS 192

Query: 272 VSIGGGPSEFFEDPIAIGSFHAMEKGILTSCSAGNSGPELRTVENVAPWIMTVAASTIDR 331
           VSIGG   EFF DPIAIGSFHAMEKGILTSCSAGNSGP+L+TVEN APWIMTVAASTIDR
Sbjct: 193 VSIGGESMEFFNDPIAIGSFHAMEKGILTSCSAGNSGPDLKTVENTAPWIMTVAASTIDR 252

Query: 332 DFSTLLKLGNNKKFAGVSVNTFSPKKKMYPLISGAKAALPEHGS---LDPRWCDYGSLDE 391
           DFST++KLGNNKK +GVSVNTF+PKK+MYPLISG+ AALP   S   L P WCDYGSLDE
Sbjct: 253 DFSTVVKLGNNKKLSGVSVNTFTPKKQMYPLISGSNAALPNQSSDLDLYPSWCDYGSLDE 312

Query: 392 NKVKGKIVYCLGSLYQEYTISDLGGVGVIANLMNLTEIAIITPIPSTHLSSQHSNFVDAY 451
            KVKGKIVYCLGS+ QEYTISDLGGVGVI+NL+N+TE+AI TPIPSTHLSS +S++V+AY
Sbjct: 313 KKVKGKIVYCLGSIDQEYTISDLGGVGVISNLLNITEMAITTPIPSTHLSSTNSDYVEAY 372

Query: 452 INSTKNPQAVILKTTTKKTEAPFLAYFSSRGPQFVAHNILKPDIAAPGMNILAAYSKLAT 511
           INSTKNP+AVI KTTT+K +APFLAYFSS+GPQ +A NILKPDIAAPG+NILAAYS LA+
Sbjct: 373 INSTKNPKAVIYKTTTRKVDAPFLAYFSSKGPQTIALNILKPDIAAPGVNILAAYSNLAS 432

Query: 512 IPIDRHATFSIMSGTSMACPHAAAAAAYLKTFHPTWSPAAIKSALMTTATPLKIGERKDE 571
           IP +RH+ F+++SGTSMACPHAAAAAAYLK FHPTWSPAA+KSALMTTATPLKIG+  D 
Sbjct: 433 IPDNRHSLFNLLSGTSMACPHAAAAAAYLKAFHPTWSPAALKSALMTTATPLKIGDELDV 492

Query: 572 LGAGAGQINPTKAIHPGLIFNISHNSYLSFLCSKNHYDGSALAILAGNSSFNCSDLTLPS 631
           +GAGAGQINPTKA+HPGLI+++S  SYLSFLC+   Y  SALAIL G++S NCSD+   S
Sbjct: 493 IGAGAGQINPTKAVHPGLIYDLSRTSYLSFLCTNKRYSDSALAILTGDASLNCSDVPQAS 552

Query: 632 GSDGLNYPSMYIPIELGATAVSAIFKRVATHVGFGPSTYKAKVKSPAGVSVRVLPETLKF 691
           GSD +NYPSMY+P++  AT+VSA+F R  THVGFGPSTYKAK+KSPAG+SV+V PETLKF
Sbjct: 553 GSDAINYPSMYVPVDRDATSVSAVFHRTVTHVGFGPSTYKAKIKSPAGLSVKVSPETLKF 612

Query: 692 NQSYETRSFKVMVKGTVAAEGSLPMKASLEWNDSKHNVRIPILLFRV 725
           +++Y+  SFKV+VKG   A G  P+ ASLEW+DSKH VR PIL+F+V
Sbjct: 613 DRAYKKLSFKVVVKGAAPAVGQAPLTASLEWDDSKHYVRSPILVFKV 659

BLAST of Tan0017032 vs. ExPASy TrEMBL
Match: A0A0A0LE00 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G775270 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 9.2e-269
Identity = 463/648 (71.45%), Postives = 544/648 (83.95%), Query Frame = 0

Query: 92  AKEKSVVSVFESKRRKLHTTRSWDFLGLSEVASRRNAAVESKIIVGMLDSGIWMDSPSFN 151
           A+EK VVSVF SK RKLHTTRSWDFLGLSE  SRRNAA ES +IVG+LDSGIWM+ PSF 
Sbjct: 9   AEEKKVVSVFRSKTRKLHTTRSWDFLGLSEAVSRRNAAAESNVIVGLLDSGIWMEGPSFK 68

Query: 152 DNGYPQIPSNWKGDCVIGHNFTGCNRKVIGARFFNLENIDNTINQSPVDEMGHGTHTSST 211
           D+GY +IPS WKG CV G NFT CNRKVIGARFF++  IDN+I++SP DE+GHG+HT+ST
Sbjct: 69  DDGYGEIPSKWKGKCVTGRNFTSCNRKVIGARFFDIGQIDNSIDKSPADEIGHGSHTAST 128

Query: 212 VAGSTVYGASLDGVAEGTARG-----------VCWSIGCTDTDLLAGFDHAIADGVDIIS 271
           +AG++V GAS  GVA GTARG           VCW  GC+D DLLAGFDHAIADGVDIIS
Sbjct: 129 IAGASVDGASFYGVAGGTARGGVPGARIAMYKVCWVDGCSDVDLLAGFDHAIADGVDIIS 188

Query: 272 VSIGGGPSEFFEDPIAIGSFHAMEKGILTSCSAGNSGPELRTVENVAPWIMTVAASTIDR 331
           VSIGG  +EFF DPIAIGSFHAMEKGILTSCSAGNSGPEL+TVEN APWIMTVAASTIDR
Sbjct: 189 VSIGGESTEFFNDPIAIGSFHAMEKGILTSCSAGNSGPELKTVENTAPWIMTVAASTIDR 248

Query: 332 DFSTLLKLGNNKKFAGVSVNTFSPKKKMYPLISGAKAALPEHGS--LDPRWCDYGSLDEN 391
           DFST++KLGNNKK +GVSVNTF+PKK+MYPLISG+ AALP      LDP WCD G+LDE 
Sbjct: 249 DFSTVVKLGNNKKLSGVSVNTFTPKKQMYPLISGSNAALPNQSDPYLDPSWCDSGTLDEK 308

Query: 392 KVKGKIVYCLGSLYQEYTISDLGGVGVIANLMNLTEIAIITPIPSTHLSSQHSNFVDAYI 451
           KVKGKIVYCLGS+ QEYTIS+LGG GVI+NLMN++E AI TPIPSTHLSS +S++V+AYI
Sbjct: 309 KVKGKIVYCLGSMDQEYTISELGGKGVISNLMNVSETAITTPIPSTHLSSTNSDYVEAYI 368

Query: 452 NSTKNPQAVILKTTTKKTEAPFLAYFSSRGPQFVAHNILKPDIAAPGMNILAAYSKLATI 511
           NSTKNP+AVI KTTT+K +AP+LA FSS+GPQ +A NILKPDIAAPG+NILAAYS LA+I
Sbjct: 369 NSTKNPKAVIYKTTTRKVDAPYLASFSSKGPQTIALNILKPDIAAPGVNILAAYSNLASI 428

Query: 512 PIDRHATFSIMSGTSMACPHAAAAAAYLKTFHPTWSPAAIKSALMTTATPLKIGERKDEL 571
             +RH+ F+++SGTSMA PH+AAAAAYLK FHPTWSPAA+KSALMTTATPLKIG++ D +
Sbjct: 429 TNNRHSLFNLLSGTSMAFPHSAAAAAYLKAFHPTWSPAALKSALMTTATPLKIGDKLDVI 488

Query: 572 GAGAGQINPTKAIHPGLIFNISHNSYLSFLCSKNHY--DGSALAILAGNSSFNCSDLTLP 631
           GAG GQINP KA+HPGLI++++  SYLSFLC+   Y   GSALAIL G++S NCSD+   
Sbjct: 489 GAGTGQINPIKAVHPGLIYDLTRTSYLSFLCTNKRYSDSGSALAILTGDTSLNCSDVPRA 548

Query: 632 SGSDGLNYPSMYIPIELGATAVSAIFKRVATHVGFGPSTYKAKVKSPAGVSVRVLPETLK 691
           SG D +NYPSMY+P++  AT+VSA+F R  THVGFGPSTY AKVKSPAG+SV+V P+TLK
Sbjct: 549 SGFDAINYPSMYVPVDRNATSVSAVFHRTVTHVGFGPSTYIAKVKSPAGLSVKVSPDTLK 608

Query: 692 FNQSYETRSFKVMVKGTVAAEGSLPMKASLEWNDSKHNVRIPILLFRV 725
           F+++Y+  SFKV+VKG   A G  P+ ASLEW+DSKH VR PIL+F+V
Sbjct: 609 FDRAYKKLSFKVVVKGAAPAVGQAPLTASLEWDDSKHYVRSPILVFKV 656

BLAST of Tan0017032 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 743.4 bits (1918), Expect = 1.7e-214
Identity = 395/759 (52.04%), Postives = 530/759 (69.83%), Query Frame = 0

Query: 2   NYKLPKIFLTFIFLSAAVASST---HVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAV 61
           N ++    L F  ++ AV ++T   +V R+ Y+VYMG   +    E  A++HH+LL   +
Sbjct: 4   NQRVRLFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEA-AENHHNLLMTVI 63

Query: 62  GDDELARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLG 121
           GD+  AR+ KIYSYG++ NGF ARL PHEA+KL++E+ VVSVF++ +R+LHTTRSWDFLG
Sbjct: 64  GDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLG 123

Query: 122 LSEVASRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRK 181
           L E   +R+  +ES IIVG+LD+GI ++SPSFND G    P+ WKG CV G+NFT CN K
Sbjct: 124 LVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNK 183

Query: 182 VIGARFFNL--ENIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG---- 241
           VIGA++F++  E + +    +  D  GHGTHTSST+AG +V  ASL G+A GTARG    
Sbjct: 184 VIGAKYFHIQSEGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPS 243

Query: 242 -------VCWSIGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEK 301
                  VCW  GCTD D+LA FD AI+DGVDIIS+SIGG    FFEDPIAIG+FHAM++
Sbjct: 244 ARIAAYKVCWDSGCTDMDMLAAFDEAISDGVDIISISIGGASLPFFEDPIAIGAFHAMKR 303

Query: 302 GILTSCSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPK 361
           GILT+CSAGN+GP L TV N+APW+MTVAA+++DR F T++KLGN    +G+S+N F+P+
Sbjct: 304 GILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPR 363

Query: 362 KKMYPLISGAKAA-LPEHGSLDPRWCDYGSLDENKVKGKIVYC--------LGSLYQEYT 421
           KKMYPL SG+ A+ L   G  +P  C+ G+L E+KV GK+VYC         G   Q++ 
Sbjct: 364 KKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHV 423

Query: 422 ISDLGGVGVIANLMNLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKT 481
           +  L G GVI  L+  T++A  T I  +++  +    +  YINSTKNPQAVI KT T K 
Sbjct: 424 VRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTKM 483

Query: 482 EAPFLAYFSSRGPQFVAHNILKPDIAAPGMNILAAYSKLATI---PID-RHATFSIMSGT 541
            AP ++ FS+RGPQ ++ NILKPDI+APG+NILAAYSKLA++   P D R   FSIMSGT
Sbjct: 484 LAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGT 543

Query: 542 SMACPHAAAAAAYLKTFHPTWSPAAIKSALMTTATPLKIGERKDELGAGAGQINPTKAIH 601
           SMACPHAAAAAAY+K+FHP WSPAAIKSALMTTATP++I   + EL  G+GQINP +AIH
Sbjct: 544 SMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGNEAELSYGSGQINPRRAIH 603

Query: 602 PGLIFNISHNSYLSFLCSKNHYDGSALAILAGNSS-------FNCSDLTLPSGSDGLNYP 661
           PGL+++I+ ++YL FLC K  Y+ +++ +L G++S       +NC ++    GSDGLNYP
Sbjct: 604 PGLVYDITEDAYLRFLC-KEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYP 663

Query: 662 SMYIPIELGATAVSAIFKRVATHVGFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRS 721
           S++  +      VS +F R  T+VG+GPSTY A+V +P G+ V V+P+ + F +  E R+
Sbjct: 664 SLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRN 723

Query: 722 FKVMVKGTVAAEGSLPMKASLEWNDSK-HNVRIPILLFR 724
           FKV++ G         + AS+EW+DS+ H VR PILLFR
Sbjct: 724 FKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLFR 760

BLAST of Tan0017032 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 587.0 bits (1512), Expect = 2.0e-167
Identity = 332/741 (44.80%), Postives = 465/741 (62.75%), Query Frame = 0

Query: 2   NYKLPKIFLTFIFLSAAVASS-THVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGD 61
           ++ L  + +  +++S   AS+     +  Y++Y+G  P   + E     H +LL++    
Sbjct: 9   HHHLLVLVMVVLWISPRYASAEDEHAKDFYIIYLGDRP--DNTEETIKTHINLLSSLNIS 68

Query: 62  DELARKSKIYSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLS 121
            E A++ K+YSY ++FN FAA+L PHEAKK+ + + VVSV  ++ RKLHTT+SWDF+GL 
Sbjct: 69  QEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGL- 128

Query: 122 EVASRRNAAVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVI 181
            + ++R+   E  +I+G+LD+GI  DS SF D+G    P+ WKG C    NFTGCN K+I
Sbjct: 129 PLTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKII 188

Query: 182 GARFFNLE-NIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG------- 241
           GA++F  + N+     +SP+D  GHGTHTSSTVAG  V  ASL G+A GTARG       
Sbjct: 189 GAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARL 248

Query: 242 ----VCWS-IGCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFEDPIAIGSFHAMEKGI 301
               VCW+  GC D D+LAGF+ AI DGV+IIS+SIGG  +++  D I++GSFHAM KGI
Sbjct: 249 AMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGI 308

Query: 302 LTSCSAGNSGPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKK 361
           LT  SAGN GP   TV N  PWI+TVAAS IDR F + + LGN K F+G+ ++ FSPK K
Sbjct: 309 LTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAK 368

Query: 362 MYPLISGAKAALPEHGSLDPRWCDYGSLDENKVKGKIVYC-LGSLYQEYTISDLGGVGVI 421
            YPL+SG  AA         R+C   SLD  KVKGK++ C +G    E TI   GG G I
Sbjct: 369 SYPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAI 428

Query: 422 ANLMNLTEIAIITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKTTTKKTEAPFLAYFSS 481
                  + A I   P+T ++S   + +  YINST++  AVI KT      APF+A FSS
Sbjct: 429 IVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSS 488

Query: 482 RGPQFVAHNILKPDIAAPGMNILAAYS-KLATIPID---RHATFSIMSGTSMACPHAAAA 541
           RGP   +  +LKPDIAAPG++ILAA++ K +   +D   + + F+I+SGTSMACPH A  
Sbjct: 489 RGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGV 548

Query: 542 AAYLKTFHPTWSPAAIKSALMTTATPLKIGERKD-ELGAGAGQINPTKAIHPGLIFNISH 601
           AAY+K+FHP W+PAAIKSA++T+A P+     KD E   G GQINP +A  PGL++++  
Sbjct: 549 AAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDD 608

Query: 602 NSYLSFLCSKNHYDGSALAILAGNSSFNCSDLTLPSGSDGLNYPSMYIPIELGATAVSAI 661
            SY+ FLC +  Y+ + LA L G  S +CS +    G D LNYP++ + +    T+  A+
Sbjct: 609 ISYVQFLCGEG-YNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAV 668

Query: 662 FKRVATHVGFGPSTYKAKVKSPAGVSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLP 721
           F+R  T+VG   S Y A V++P GV + V P++L F+++ + RSFKV+VK      G + 
Sbjct: 669 FRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKI- 728

Query: 722 MKASLEWNDSKHNVRIPILLF 723
           +   L W   +H+VR PI+++
Sbjct: 729 VSGLLVWKSPRHSVRSPIVIY 744

BLAST of Tan0017032 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 518.1 bits (1333), Expect = 1.2e-146
Identity = 315/713 (44.18%), Postives = 423/713 (59.33%), Query Frame = 0

Query: 34  MGALPKLQSHEVLADHHHSLLANAVGDDELARKSKIYSYGRSFNGFAARLLPHEAKKLAK 93
           MG LP+++       HH S+L   VG    A    + SY RSFNGFAA L   E++KL  
Sbjct: 1   MGTLPEIKYSP--PSHHLSILQKLVG-TIAASHLLVRSYKRSFNGFAANLSQAESQKLQN 60

Query: 94  EKSVVSVFESKRRKLHTTRSWDFLGLSEVASRRNAAVESKIIVGMLDSGIWMDSPSFNDN 153
            K VVSVF SK  +L TTRSWDF+G  E A RR +  ES +IVG++DSGIW +S SF+D 
Sbjct: 61  MKEVVSVFPSKSHELTTTRSWDFVGFGEKA-RRESVKESDVIVGVIDSGIWPESESFDDE 120

Query: 154 GYPQIPSNWKGDCVIGHNFTGCNRKVIGARFFNLENIDNTINQSPVDEMGHGTHTSSTVA 213
           G+   P  WKG C  G  F  CN K+IGARF+      N    S  DE GHGTHT+ST A
Sbjct: 121 GFGPPPKKWKGSCKGGLKF-ACNNKLIGARFY------NKFADSARDEEGHGTHTASTAA 180

Query: 214 GSTVYGASLDGVAEGTARG-----------VCWSIGCTDTDLLAGFDHAIADGVDIISVS 273
           G+ V  AS  G+A+GTARG           VC++  C D D+LA FD AIADGVD+IS+S
Sbjct: 181 GNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN-RCNDVDILAAFDDAIADGVDVISIS 240

Query: 274 IGGG-PSEFFEDPIAIGSFHAMEKGILTSCSAGNSGPELRTVENVAPWIMTVAASTIDRD 333
           I     S      +AIGSFHAM +GI+T+ SAGN+GP+  +V NV+PW++TVAAS  DR 
Sbjct: 241 ISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQ 300

Query: 334 FSTLLKLGNNKKFAGVSVNTFSPKKKMYPLISGAKAALPEHGSLDPRWCDYGSLDENKVK 393
           F   + LGN K   G+SVNTF+     +P++ G   +          +C  G +D   VK
Sbjct: 301 FIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVS-RNCSQAQAGYCSSGCVDSELVK 360

Query: 394 GKIVYCLGSL-YQEYTISDLGGVGVIANLMNLTEIAIITPIPSTHLSSQHSNFVDAYINS 453
           GKIV C   L Y+E  ++  G +GVI     L + A + P P++ L  +    + +YI S
Sbjct: 361 GKIVLCDDFLGYREAYLA--GAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIES 420

Query: 454 TKNPQAVILKT-TTKKTEAPFLAYFSSRGPQFVAHNILKPDIAAPGMNILAAYSKLAT-- 513
            + PQA IL+T      EAP++  FSSRGP FV  N+LKPD++APG+ ILAA+S +A+  
Sbjct: 421 AEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPS 480

Query: 514 ---IPID-RHATFSIMSGTSMACPHAAAAAAYLKTFHPTWSPAAIKSALMTTATPLKIGE 573
               P D R   +S+MSGTSMACPH A  AAY+K+FHP WSP+AIKSA+MTTATP+ + +
Sbjct: 481 SFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKK 540

Query: 574 R-KDELGAGAGQINPTKAIHPGLIFNISHNSYLSFLCSKNHYDGSALAILAGNSSFNCSD 633
             + E   G+GQINPTKA  PGL++ +    YL  LC++  +D + L   +G  +  CS+
Sbjct: 541 NPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEG-FDSTTLTTTSG-QNVTCSE 600

Query: 634 LTLPSGSDGLNYPSMYIPIELGATAVSAIFKRVATHVGFGPSTYKAK-VKSPAGVSVRVL 693
            T       LNYP+M   +       +  FKR  T+VGF  STYKA  V     + + + 
Sbjct: 601 RT---EVKDLNYPTMTTFVS-SLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIE 660

Query: 694 PETLKFNQSYETRSFKVMVKGTVAAEGSLPMKASLEWNDSKHNVRIPILLFRV 725
           PE L+F    E +SF V + G    +GS  + +S+ W+D  H+VR PI+ + +
Sbjct: 661 PEILRFGFLEEKKSFVVTISGKELKDGSF-VSSSVVWSDGSHSVRSPIVAYSI 691

BLAST of Tan0017032 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 517.3 bits (1331), Expect = 2.0e-146
Identity = 312/736 (42.39%), Postives = 433/736 (58.83%), Query Frame = 0

Query: 10  LTFIFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGDDELARKSKI 69
           L  +FLS+   S+    +Q Y+VYMG+L     +   +D H ++L    G+  +  +  +
Sbjct: 14  LLVLFLSS--VSAVTDDKQVYIVYMGSLSSRADYTPTSD-HMNILQEVTGESSIEGR-LV 73

Query: 70  YSYGRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLSE-VASRRNA 129
            SY RSFNGFAARL   E +++AK   VVSVF +K+ +L TT SWDF+GL E + ++RN 
Sbjct: 74  RSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNP 133

Query: 130 AVESKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVIGARFFNLE 189
            VES  I+G++DSGI  +S SF+D G+   P  WKG C  G NFT CN K+IGAR +  E
Sbjct: 134 TVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT-CNNKLIGARDYTSE 193

Query: 190 NIDNTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG-----------VCWSI 249
                      D  GHGTHT+ST AG+ V  AS  G+  GT RG           VC   
Sbjct: 194 GTR--------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPT 253

Query: 250 GCTDTDLLAGFDHAIADGVDIISVSIGGGPSEFFE-DPIAIGSFHAMEKGILTSCSAGNS 309
           GC+   LL+ FD AIADGVD+I++SIG   +  F+ DPIAIG+FHAM KG+LT  SAGNS
Sbjct: 254 GCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNS 313

Query: 310 GPELRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKKMYPLISGAK 369
           GP+  +V  VAPWI+TVAAST +R F T + LGN K   G SVN +  K K YPL+ G  
Sbjct: 314 GPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKS 373

Query: 370 AALPEHGSLDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVGVIANLMNLTEIA 429
           AA     +     C+   +D+++VKGKI+ C G       +  +G VG+I       ++A
Sbjct: 374 AASSACDAESAGLCELSCVDKSRVKGKILVC-GGPGGLKIVESVGAVGLIYRTPK-PDVA 433

Query: 430 IITPIPSTHLSSQHSNFVDAYINSTKNPQAVILKT-TTKKTEAPFLAYFSSRGPQFVAHN 489
            I P+P+  L ++    + +Y+ ST +PQA++LKT       +P +A FSSRGP  +A +
Sbjct: 434 FIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVD 493

Query: 490 ILKPDIAAPGMNILAAYSKLATIPID--RHATFSIMSGTSMACPHAAAAAAYLKTFHPTW 549
           ILKPDI APG+ ILAAYS       D  RH  +S++SGTSM+CPH A  AAY+KTF+P W
Sbjct: 494 ILKPDITAPGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKW 553

Query: 550 SPAAIKSALMTTATPLK---IGERKDELGAGAGQINPTKAIHPGLIFNISHNSYLSFLCS 609
           SP+ I+SA+MTTA P+     G    E   G+G ++P  A +PGL++ +  + +++FLC 
Sbjct: 554 SPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCG 613

Query: 610 KNHYDGSALAILAGNSSFNCSDL--TLPSGSDGLNYPSMYIPIELGATAVSAIFKRVATH 669
            N Y    L +++G  +  CS+    LP     LNYPSM   +    T  +  F R  T+
Sbjct: 614 MN-YTSQVLKVISG-ETVTCSEAKKILPR---NLNYPSMSAKLSGSGTTFTVTFNRTLTN 673

Query: 670 VGFGPSTYKAKVKSPAG--VSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKASL 723
           VG   STY +KV +  G  + V++ P  L F    E +SF V V G+   +  +P  A+L
Sbjct: 674 VGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGS-NLDSEVPSSANL 728

BLAST of Tan0017032 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 517.3 bits (1331), Expect = 2.0e-146
Identity = 317/733 (43.25%), Postives = 431/733 (58.80%), Query Frame = 0

Query: 13  IFLSAAVASSTHVPRQAYVVYMGALPKLQSHEVLADHHHSLLANAVGDDELARKSKIYSY 72
           + L ++V++      Q Y+VYMG+L     +   +D H S+L    G+  +  +  + SY
Sbjct: 16  VLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSD-HMSILQQVTGESSIEGR-LVRSY 75

Query: 73  GRSFNGFAARLLPHEAKKLAKEKSVVSVFESKRRKLHTTRSWDFLGLSE-VASRRNAAVE 132
            RSFNGFAARL   E   +A+ + VVSVF +K  +LHTT SWDF+G+ E   ++RN A+E
Sbjct: 76  KRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIE 135

Query: 133 SKIIVGMLDSGIWMDSPSFNDNGYPQIPSNWKGDCVIGHNFTGCNRKVIGARFFNLENID 192
           S  I+G++D+GIW +S SF+D G+   P  WKG C  G NFT CN K+IGAR +  E   
Sbjct: 136 SDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT-CNNKLIGARDYTSEGTR 195

Query: 193 NTINQSPVDEMGHGTHTSSTVAGSTVYGASLDGVAEGTARG-----------VCWSIGCT 252
           +T         GHGTHT+ST AG+ V   S  G+  GT RG           VC   GC+
Sbjct: 196 DT--------SGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCS 255

Query: 253 DTDLLAGFDHAIADGVDIISVSIG-GGPSEFFEDPIAIGSFHAMEKGILTSCSAGNSGPE 312
              LL+ FD AIADGVD+I++SIG   PS F +DPIAIG+FHAM KGILT  SAGNSGP+
Sbjct: 256 SEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPK 315

Query: 313 LRTVENVAPWIMTVAASTIDRDFSTLLKLGNNKKFAGVSVNTFSPKKKMYPLISGAKAAL 372
             TV +VAPWI TVAAST +R F T + LGN K  AG SVN F  K K YPL+ G  AA 
Sbjct: 316 PTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAAS 375

Query: 373 PEHGSLDPRWCDYGSLDENKVKGKIVYCLGSLYQEYTISDLGGVGVIANLMNLTEIAIIT 432
               +     C    L++++VKGKI+ C G     Y I+   G   I +     ++A   
Sbjct: 376 SACDAKTAALCAPACLNKSRVKGKILVCGGP--SGYKIAKSVGAIAIIDKSPRPDVAFTH 435

Query: 433 PIPSTHLSSQHSNFVDAYINSTKNPQAVILKT-TTKKTEAPFLAYFSSRGPQFVAHNILK 492
            +P++ L ++    + +YI S  +PQA +LKT T     +P +A FSSRGP  +A +ILK
Sbjct: 436 HLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILK 495

Query: 493 PDIAAPGMNILAAYSKLATIPID--RHATFSIMSGTSMACPHAAAAAAYLKTFHPTWSPA 552
           PDI APG+ ILAA+S       D  R   +S+ SGTSMACPH A  AAY+KTF+P WSP+
Sbjct: 496 PDITAPGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPS 555

Query: 553 AIKSALMTTATPLKI---GERKDELGAGAGQINPTKAIHPGLIFNISHNSYLSFLCSKNH 612
            I+SA+MTTA P+K    G    E   GAG ++P  A++PGL++ +    +++FLC  N 
Sbjct: 556 MIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMN- 615

Query: 613 YDGSALAILAGNSSFNCS--DLTLPSGSDGLNYPSMYIPIELGATAVSAIFKRVATHVGF 672
           Y    L I++G+ +  CS  +  LP     LNYPSM   +    +  S  F R  T+VG 
Sbjct: 616 YTSKTLKIISGD-TVKCSKKNKILPR---NLNYPSMSAKLSGTDSTFSVTFNRTLTNVGT 675

Query: 673 GPSTYKAKVKSPAG--VSVRVLPETLKFNQSYETRSFKVMVKGTVAAEGSLPMKASLEWN 723
             STYK+KV +  G  +S++V P  L F    E +SF V V G+   +  +P  A+L W+
Sbjct: 676 PNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGS-DVDSEVPSSANLIWS 729