Tan0016877 (gene) Snake gourd v1

Overview


Name	Tan0016877
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	High-affinity nitrate transporter
Location	LG05: 69156502 .. 69157652 (-)
RNA-Seq Expression	Tan0016877
Synteny	Tan0016877

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTAAACTAAACCCCAATCCTTCCCTCTTTCAACTCCCAAACCTTTCTCCTCTTCTGTCTCTTCATTGCATTTTAGAGCCAAAATTTCCCTGATTCTTATTATTTAAAAGAATATTCATCCATTCTTCCTCAAATTCCCTTTCAACTTGGGCTTCTTTGACTTGTTCTCTCACCACTTTTATATAAACCAAACCAAGCCCAAACTTCTGTTATCAATTTCATCATCAAACCTGAGATTCACTTCACTTTTTTGACAGGTTACAACTTGAATCAACAATGGCTGTTCATGGGTTTCTGTTGGCTGCTCTGTTTCTCTTCTGTTTCTTGGGATTCTCTCATGGAAATATCCTTTTTTCTTCTCTCCCAAGAAGTTTGGAGGTCACTGCTTCTCCTAAACCTGGTCAAGGTGAGATTTTTCAGTTCCCATAACTCTAAATTCTGATTCTGAATCAAAAACTCAAACTTTACCAATTTCTACCAACTGGGTTCAACAAAAATTCAAACTTTACCAATATCTAACAACTGGGTTTTTTTTTTTTTTTTTTTTTTCTGCAGTTCTTAAAGCGGGTGTTGATAAAATCAGTGTGAGTTGGGTTGTAAACGGGACTGTCAAAGCCGGGTCGGATTCGGCTTACAAAACCGTCAAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAAATGAACCGCGCGTGGAGGAAAACAGAGGACCATCTCTCGAAGGACAAGACTTGCCAGTTCCAAATCGTGGCAAAGCCGTACAATCCGGCGAACAAGACGGTTCAAAAATTCGAGTGGACGGTGGCGCGTGATACTCCGACGGGGACTTACTTCGTACGCGCCTACGTTTACAATTCCGCCGGCGAAGAAGTGGCCTACGGCCAAACCACAGATAATAAAAAAAGCACAAACTTGTTTCAAATCGAATCCATCTCTGGCCGCCATATCTCCCTTGATATTGCTTCTGTTTGTTTCAGCGCTTTCTCTATCTTGTCCTTGTTCGGATTCTTCATCGCCGACAAAAGGAAGGCCAAGAAGGCGGCGGCGGCGCAGGGACAGTAAATTAAGAAAGGTGGGAACTTTGCTCTGTTTTGAGGTTCTCTTTTAAGCTTTGCATCAATGGCGATCTGATTGATAAAGAGTAGATTTTATGAG

mRNA sequence

ATTAAACTAAACCCCAATCCTTCCCTCTTTCAACTCCCAAACCTTTCTCCTCTTCTGTCTCTTCATTGCATTTTAGAGCCAAAATTTCCCTGATTCTTATTATTTAAAAGAATATTCATCCATTCTTCCTCAAATTCCCTTTCAACTTGGGCTTCTTTGACTTGTTCTCTCACCACTTTTATATAAACCAAACCAAGCCCAAACTTCTGTTATCAATTTCATCATCAAACCTGAGATTCACTTCACTTTTTTGACAGGTTACAACTTGAATCAACAATGGCTGTTCATGGGTTTCTGTTGGCTGCTCTGTTTCTCTTCTGTTTCTTGGGATTCTCTCATGGAAATATCCTTTTTTCTTCTCTCCCAAGAAGTTTGGAGGTCACTGCTTCTCCTAAACCTGGTCAAGTTCTTAAAGCGGGTGTTGATAAAATCAGTGTGAGTTGGGTTGTAAACGGGACTGTCAAAGCCGGGTCGGATTCGGCTTACAAAACCGTCAAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAAATGAACCGCGCGTGGAGGAAAACAGAGGACCATCTCTCGAAGGACAAGACTTGCCAGTTCCAAATCGTGGCAAAGCCGTACAATCCGGCGAACAAGACGGTTCAAAAATTCGAGTGGACGGTGGCGCGTGATACTCCGACGGGGACTTACTTCGTACGCGCCTACGTTTACAATTCCGCCGGCGAAGAAGTGGCCTACGGCCAAACCACAGATAATAAAAAAAGCACAAACTTGTTTCAAATCGAATCCATCTCTGGCCGCCATATCTCCCTTGATATTGCTTCTGTTTGTTTCAGCGCTTTCTCTATCTTGTCCTTGTTCGGATTCTTCATCGCCGACAAAAGGAAGGCCAAGAAGGCGGCGGCGGCGCAGGGACAGTAAATTAAGAAAGGTGGGAACTTTGCTCTGTTTTGAGGTTCTCTTTTAAGCTTTGCATCAATGGCGATCTGATTGATAAAGAGTAGATTTTATGAG

Coding sequence (CDS)

ATGGCTGTTCATGGGTTTCTGTTGGCTGCTCTGTTTCTCTTCTGTTTCTTGGGATTCTCTCATGGAAATATCCTTTTTTCTTCTCTCCCAAGAAGTTTGGAGGTCACTGCTTCTCCTAAACCTGGTCAAGTTCTTAAAGCGGGTGTTGATAAAATCAGTGTGAGTTGGGTTGTAAACGGGACTGTCAAAGCCGGGTCGGATTCGGCTTACAAAACCGTCAAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAAATGAACCGCGCGTGGAGGAAAACAGAGGACCATCTCTCGAAGGACAAGACTTGCCAGTTCCAAATCGTGGCAAAGCCGTACAATCCGGCGAACAAGACGGTTCAAAAATTCGAGTGGACGGTGGCGCGTGATACTCCGACGGGGACTTACTTCGTACGCGCCTACGTTTACAATTCCGCCGGCGAAGAAGTGGCCTACGGCCAAACCACAGATAATAAAAAAAGCACAAACTTGTTTCAAATCGAATCCATCTCTGGCCGCCATATCTCCCTTGATATTGCTTCTGTTTGTTTCAGCGCTTTCTCTATCTTGTCCTTGTTCGGATTCTTCATCGCCGACAAAAGGAAGGCCAAGAAGGCGGCGGCGGCGCAGGGACAGTAA

Protein sequence

MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNGTVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQKFEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASVCFSAFSILSLFGFFIADKRKAKKAAAAQGQ

Homology

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BLAST of Tan0016877 vs. ExPASy Swiss-Prot
Match: Q9SB67 (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.8e-49
Identity = 102/207 (49.28%), Postives = 145/207 (70.05%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHG---NILFSSLPRSLEVTASP-KPGQVLKAGVDKISVSW 60
           MA+H  L  +L +F  +  S G   + LF+ L  S+EVTA P K   VL+AG D ++++W
Sbjct: 1   MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 60

Query: 61  -VVNGTVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPAN 120
            + + + K  +D+A+KT++ KLCYAP+SQ++R WRKT++ L KD++C  +IV+K Y   +
Sbjct: 61  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---D 120

Query: 121 KTVQKFEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISL 180
           KT Q  +WT+  D PTGTYFVRAY  +  G EVAYGQ+TD  ++TNLF + +ISG H+ L
Sbjct: 121 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 180

Query: 181 DIASVCFSAFSILSLFGFFIADKRKAK 203
           DIAS  FS FS++SLF FF+ +KRKAK
Sbjct: 181 DIASTFFSVFSVVSLFVFFVMEKRKAK 204

[Close]

BLAST of Tan0016877 vs. ExPASy Swiss-Prot
Match: Q9FGS5 (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 7.0e-49
Identity = 104/209 (49.76%), Postives = 144/209 (68.90%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGN---ILFSSLPR-SLEVTASPK---PGQVLKAGVDKIS 60
           MA+   L A+L +   +   HG     LF  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VSWVVNGTVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNP 120
           ++W ++ ++ +  ++ +K +K KLCYAP SQ++R WRKT D L KDKTC  +I+AKPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHI 180
            +KT+Q   WT+ RD PTGTYFVRAY  ++ G EVAYGQ+TD+ K TNLF +++ISGRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSILSLFGFFIADKRKAK 203
           SLDIAS+CFS FS+++L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Tan0016877 vs. ExPASy Swiss-Prot
Match: Q6ZI50 (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 3.0e-39
Identity = 82/180 (45.56%), Postives = 119/180 (66.11%), Query Frame = 0


Query: 24  ILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNGTVKAGSDSAYKTVKAKLCYAPVS 83
           +  S LP++L V  SPK G+V+ AG + ++V+W +N +  AG+D+A+K+VK KLCYAP S
Sbjct: 34  VFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPAS 93

Query: 84  QMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQKFEWTVARDTPTGTYFVRAYVYNS 143
           + +R WRK  D L KDK CQF++  +PY        +F++ VARD PT +YFVRAY  ++
Sbjct: 94  RTDRGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDA 153

Query: 144 AGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASVCFSAFSILSLFGFFIADKRKAKK 203
           +G EVAYGQ++ +      F +  I+G H SL +A+  FS FSI +L  FF+ +KRK  K
Sbjct: 154 SGTEVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

[Close]

BLAST of Tan0016877 vs. ExPASy Swiss-Prot
Match: Q7XK12 (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 5.6e-38
Identity = 91/211 (43.13%), Postives = 124/211 (58.77%), Query Frame = 0


Query: 3   VHGFLLAALFLFCFLGFSH-------GNILFSSLPRSLEVTASPKPGQVLKAGVDKISVS 62
           +H   L  L L   LG  H            S+L ++L V ASP+ GQVL AG D I+V+
Sbjct: 8   IHRVFLPLLLLLVVLGACHVTPAAAAAGARLSALAKALVVEASPRAGQVLHAGEDAITVT 67

Query: 63  WVVNGT---VKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYN 122
           W +N T     AG+D+ YK VK  LCYAP SQ+ R WRK  D LSKDK CQF+I  +PY+
Sbjct: 68  WSLNATAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRKAHDDLSKDKACQFKIAQQPYD 127

Query: 123 PANKTVQKFEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRH 182
            A     KFE+TVARD PT +Y+VRAY  +++G  VAYG+T  +      F +  I+G  
Sbjct: 128 GAG----KFEYTVARDVPTASYYVRAYALDASGARVAYGETAPSAS----FAVAGITGVT 187

Query: 183 ISLDIASVCFSAFSILSLFGFFIADKRKAKK 204
            S+++A+   SAFS+ +L  F + + +K  K
Sbjct: 188 ASIEVAAGVLSAFSVAALAVFLVLENKKKNK 210

[Close]

BLAST of Tan0016877 vs. NCBI nr
Match: XP_023520111.1 (high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 356.7 bits (914), Expect = 1.4e-94
Identity = 177/206 (85.92%), Postives = 193/206 (93.69%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNG 60
           M+ +GFLLAALFLFCF GFSHG+I FSSLPRSLEVTASPKPGQVLKAGVDKISV+W++NG
Sbjct: 7   MSANGFLLAALFLFCFFGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWILNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQK 120
           TVK+GSDS+YKTV+AKLCYAPVSQ++RAWRKTED LSKDKTCQF IV KPYNPANKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126

Query: 121 FEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASV 180
           FEWTVARD PTGT+FVRAY +NSAGEEV YGQTTDNKK TNLFQIESI+GRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIESITGRHISLDIASV 186

Query: 181 CFSAFSILSLFGFFIADKRKAKKAAA 207
           CFSAFS+LSL GFF+A KRKAKKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212

[Close]

BLAST of Tan0016877 vs. NCBI nr
Match: XP_022924070.1 (high-affinity nitrate transporter 3.1 [Cucurbita moschata])

HSP 1 Score: 354.8 bits (909), Expect = 5.3e-94
Identity = 176/206 (85.44%), Postives = 193/206 (93.69%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNG 60
           M+ +GFLLAALFLFCFLGFSHG+I FSSLPRSLEVTASPKPGQVLKAGVDKISV+WV+NG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQK 120
           TVK+GSDS+YKTV+AKLCYAPVSQ++RAWRKTED LSKDKTCQF IV KPYNP NKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 FEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASV 180
           FEWTVARD PTGT+FVRAY +NSAG+EV YGQTTDNKK TNLFQIE+I+GRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSILSLFGFFIADKRKAKKAAA 207
           CFSAFS+LSL GFF+A KRKAKKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212

[Close]

BLAST of Tan0016877 vs. NCBI nr
Match: KAG6584111.1 (High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019702.1 High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 353.6 bits (906), Expect = 1.2e-93
Identity = 176/208 (84.62%), Postives = 193/208 (92.79%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNG 60
           M+ +GFLLAALFL CFLGFSHG+I FSSLPRSLEVTASPKPGQVLKAGVDKISV+WV+NG
Sbjct: 7   MSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQK 120
           TVK+GSDS+YKTV+AKLCYAPVSQ++RAWRKTED LSKDKTCQF IV KPYNP NKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 FEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASV 180
           FEWTVARD PTGT+FVRAY +NSAGEEV YGQTTDNKK TNLFQIE+I+GRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSILSLFGFFIADKRKAKKAAAAQ 209
           CFSAFS+LSL GFF+A KRKAKKA A +
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEAPE 214

[Close]

BLAST of Tan0016877 vs. NCBI nr
Match: XP_023001304.1 (high-affinity nitrate transporter 3.1-like [Cucurbita maxima])

HSP 1 Score: 352.8 bits (904), Expect = 2.0e-93
Identity = 175/206 (84.95%), Postives = 193/206 (93.69%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNG 60
           M+ +GFLLAALFLFCFLGFSHG+I FSSLPRSLEVTASPKPGQVLKAGVDKISV+WV+NG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQK 120
           TVK+GSDS+YKTV+AKLCYAPVSQ++RAWRKTED LSKDKTCQF IV KPYNPANKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126

Query: 121 FEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASV 180
           FEWTVARD PTGT+FVRAY +NSAGEEV YGQT+DNKK TNLFQI++I+GRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186

Query: 181 CFSAFSILSLFGFFIADKRKAKKAAA 207
           CFSAFS+LSL GFF+A KRK KKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKTKKAEA 212

[Close]

BLAST of Tan0016877 vs. NCBI nr
Match: XP_008439011.1 (PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa] >TYJ99190.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa])

HSP 1 Score: 347.1 bits (889), Expect = 1.1e-91
Identity = 173/207 (83.57%), Postives = 189/207 (91.30%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNG 60
           MA +GFLLAALFL CFLGFS GNI  SSLPR+LEVTASPKPGQVLKAGVDKI V+WV+NG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQK 120
           TVKAGSDS+YK +KAKLCYAPVSQ++RAWRKTED L KDKTCQF IV KPYNPANKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASV 180
           FEWTV RD PTGT+FVRAYV+NSAG+EVAYGQTTD+KK TNLFQIESI+GRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSILSLFGFFIADKRKAKKAAAA 208
           CFSAFS++SLFGFF  DKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of Tan0016877 vs. ExPASy TrEMBL
Match: A0A6J1EDS1 (High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.6e-94
Identity = 176/206 (85.44%), Postives = 193/206 (93.69%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNG 60
           M+ +GFLLAALFLFCFLGFSHG+I FSSLPRSLEVTASPKPGQVLKAGVDKISV+WV+NG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQK 120
           TVK+GSDS+YKTV+AKLCYAPVSQ++RAWRKTED LSKDKTCQF IV KPYNP NKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 FEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASV 180
           FEWTVARD PTGT+FVRAY +NSAG+EV YGQTTDNKK TNLFQIE+I+GRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSILSLFGFFIADKRKAKKAAA 207
           CFSAFS+LSL GFF+A KRKAKKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212

[Close]

BLAST of Tan0016877 vs. ExPASy TrEMBL
Match: A0A6J1KMC9 (High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 9.7e-94
Identity = 175/206 (84.95%), Postives = 193/206 (93.69%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNG 60
           M+ +GFLLAALFLFCFLGFSHG+I FSSLPRSLEVTASPKPGQVLKAGVDKISV+WV+NG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQK 120
           TVK+GSDS+YKTV+AKLCYAPVSQ++RAWRKTED LSKDKTCQF IV KPYNPANKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126

Query: 121 FEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASV 180
           FEWTVARD PTGT+FVRAY +NSAGEEV YGQT+DNKK TNLFQI++I+GRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186

Query: 181 CFSAFSILSLFGFFIADKRKAKKAAA 207
           CFSAFS+LSL GFF+A KRK KKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKTKKAEA 212

[Close]

BLAST of Tan0016877 vs. ExPASy TrEMBL
Match: A0A5A7ULV7 (High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004000 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 5.3e-92
Identity = 173/207 (83.57%), Postives = 189/207 (91.30%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNG 60
           MA +GFLLAALFL CFLGFS GNI  SSLPR+LEVTASPKPGQVLKAGVDKI V+WV+NG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQK 120
           TVKAGSDS+YK +KAKLCYAPVSQ++RAWRKTED L KDKTCQF IV KPYNPANKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASV 180
           FEWTV RD PTGT+FVRAYV+NSAG+EVAYGQTTD+KK TNLFQIESI+GRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSILSLFGFFIADKRKAKKAAAA 208
           CFSAFS++SLFGFF  DKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of Tan0016877 vs. ExPASy TrEMBL
Match: A0A1S3AXC1 (High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 5.3e-92
Identity = 173/207 (83.57%), Postives = 189/207 (91.30%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNG 60
           MA +GFLLAALFL CFLGFS GNI  SSLPR+LEVTASPKPGQVLKAGVDKI V+WV+NG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQK 120
           TVKAGSDS+YK +KAKLCYAPVSQ++RAWRKTED L KDKTCQF IV KPYNPANKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASV 180
           FEWTV RD PTGT+FVRAYV+NSAG+EVAYGQTTD+KK TNLFQIESI+GRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSILSLFGFFIADKRKAKKAAAA 208
           CFSAFS++SLFGFF  DKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of Tan0016877 vs. ExPASy TrEMBL
Match: C8CHG8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2)

HSP 1 Score: 345.5 bits (885), Expect = 1.5e-91
Identity = 175/207 (84.54%), Postives = 187/207 (90.34%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGNILFSSLPRSLEVTASPKPGQVLKAGVDKISVSWVVNG 60
           MA + FLLAALFL CFLGFSHGNI FSSLPR+LEVTASPKPGQVLKAGVDKISV+WV+N 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPANKTVQK 120
           TVKAGSDS+YK +KAKLCYAPVSQ++RAWRKTED L KDKTCQF IV K YNPANKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 FEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISLDIASV 180
           FEWTV RD PTGT+FVRAYV NSAGEEVAYGQTTD KK TNLFQIESI+GRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSILSLFGFFIADKRKAKKAAAA 208
           CFSAFS++SLFGFF  DKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of Tan0016877 vs. TAIR 10
Match: AT4G24730.4 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 197.2 bits (500), Expect = 1.3e-50
Identity = 102/207 (49.28%), Postives = 145/207 (70.05%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHG---NILFSSLPRSLEVTASP-KPGQVLKAGVDKISVSW 60
           MA+H  L  +L +F  +  S G   + LF+ L  S+EVTA P K   VL+AG D ++++W
Sbjct: 23  MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 82

Query: 61  -VVNGTVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNPAN 120
            + + + K  +D+A+KT++ KLCYAP+SQ++R WRKT++ L KD++C  +IV+K Y   +
Sbjct: 83  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---D 142

Query: 121 KTVQKFEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHISL 180
           KT Q  +WT+  D PTGTYFVRAY  +  G EVAYGQ+TD  ++TNLF + +ISG H+ L
Sbjct: 143 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 202

Query: 181 DIASVCFSAFSILSLFGFFIADKRKAK 203
           DIAS  FS FS++SLF FF+ +KRKAK
Sbjct: 203 DIASTFFSVFSVVSLFVFFVMEKRKAK 226

[Close]

BLAST of Tan0016877 vs. TAIR 10
Match: AT5G50200.3 (nitrate transmembrane transporters )

HSP 1 Score: 195.3 bits (495), Expect = 5.0e-50
Identity = 104/209 (49.76%), Postives = 144/209 (68.90%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGN---ILFSSLPR-SLEVTASPK---PGQVLKAGVDKIS 60
           MA+   L A+L +   +   HG     LF  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VSWVVNGTVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNP 120
           ++W ++ ++ +  ++ +K +K KLCYAP SQ++R WRKT D L KDKTC  +I+AKPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHI 180
            +KT+Q   WT+ RD PTGTYFVRAY  ++ G EVAYGQ+TD+ K TNLF +++ISGRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSILSLFGFFIADKRKAK 203
           SLDIAS+CFS FS+++L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Tan0016877 vs. TAIR 10
Match: AT5G50200.2 (nitrate transmembrane transporters )

HSP 1 Score: 195.3 bits (495), Expect = 5.0e-50
Identity = 104/209 (49.76%), Postives = 144/209 (68.90%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGN---ILFSSLPR-SLEVTASPK---PGQVLKAGVDKIS 60
           MA+   L A+L +   +   HG     LF  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VSWVVNGTVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNP 120
           ++W ++ ++ +  ++ +K +K KLCYAP SQ++R WRKT D L KDKTC  +I+AKPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHI 180
            +KT+Q   WT+ RD PTGTYFVRAY  ++ G EVAYGQ+TD+ K TNLF +++ISGRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSILSLFGFFIADKRKAK 203
           SLDIAS+CFS FS+++L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Tan0016877 vs. TAIR 10
Match: AT5G50200.1 (nitrate transmembrane transporters )

HSP 1 Score: 195.3 bits (495), Expect = 5.0e-50
Identity = 104/209 (49.76%), Postives = 144/209 (68.90%), Query Frame = 0


Query: 1   MAVHGFLLAALFLFCFLGFSHGN---ILFSSLPR-SLEVTASPK---PGQVLKAGVDKIS 60
           MA+   L A+L +   +   HG     LF  L + +L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VSWVVNGTVKAGSDSAYKTVKAKLCYAPVSQMNRAWRKTEDHLSKDKTCQFQIVAKPYNP 120
           ++W ++ ++ +  ++ +K +K KLCYAP SQ++R WRKT D L KDKTC  +I+AKPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVARDTPTGTYFVRAYVYNSAGEEVAYGQTTDNKKSTNLFQIESISGRHI 180
            +KT+Q   WT+ RD PTGTYFVRAY  ++ G EVAYGQ+TD+ K TNLF +++ISGRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSILSLFGFFIADKRKAK 203
           SLDIAS+CFS FS+++L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Tan0016877 vs. TAIR 10
Match: AT4G24730.1 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-45
Identity = 89/173 (51.45%), Postives = 125/173 (72.25%), Query Frame = 0


Query: 31  RSLEVTASPKPGQVLKAGVDKISVSW-VVNGTVKAGSDSAYKTVKAKLCYAPVSQMNRAW 90
           RSLE      P  VL+AG D ++++W + + + K  +D+A+KT++ KLCYAP+SQ++R W
Sbjct: 271 RSLEAALECPP--VLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPW 330

Query: 91  RKTEDHLSKDKTCQFQIVAKPYNPANKTVQKFEWTVARDTPTGTYFVRAYVYNSAGEEVA 150
           RKT++ L KD++C  +IV+K Y   +KT Q  +WT+  D PTGTYFVRAY  +  G EVA
Sbjct: 331 RKTDNKLFKDRSCPHEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVA 390

Query: 151 YGQTTDNKKSTNLFQIESISGRHISLDIASVCFSAFSILSLFGFFIADKRKAK 203
           YGQ+TD  ++TNLF + +ISG H+ LDIAS  FS FS++SLF FF+ +KRKAK
Sbjct: 391 YGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 438

The following BLAST results are available for this feature:

BLAST of Tan0016877 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SB67	1.8e-49	49.28	High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 ...	[more]
Q9FGS5	7.0e-49	49.76	High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 ...	[more]
Q6ZI50	3.0e-39	45.56	High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ...	[more]
Q7XK12	5.6e-38	43.13	Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ...	[more]

BLAST of Tan0016877 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023520111.1	1.4e-94	85.92	high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022924070.1	5.3e-94	85.44	high-affinity nitrate transporter 3.1 [Cucurbita moschata]	[more]
KAG6584111.1	1.2e-93	84.62	High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_023001304.1	2.0e-93	84.95	high-affinity nitrate transporter 3.1-like [Cucurbita maxima]	[more]
XP_008439011.1	1.1e-91	83.57	PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266...	[more]

BLAST of Tan0016877 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EDS1	2.6e-94	85.44	High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 ...	[more]
A0A6J1KMC9	9.7e-94	84.95	High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE...	[more]
A0A5A7ULV7	5.3e-92	83.57	High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3AXC1	5.3e-92	83.57	High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 S...	[more]
C8CHG8	1.5e-91	84.54	High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2	[more]

BLAST of Tan0016877 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24730.4	1.3e-50	49.28	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]
AT5G50200.3	5.0e-50	49.76	nitrate transmembrane transporters	[more]
AT5G50200.2	5.0e-50	49.76	nitrate transmembrane transporters	[more]
AT5G50200.1	5.0e-50	49.76	nitrate transmembrane transporters	[more]
AT4G24730.1	1.3e-45	51.45	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016605	High-affinity nitrate transporter	PIRSF	PIRSF012939	NAR2	coord: 1..205 e-value: 1.6E-82 score: 273.6
IPR016605	High-affinity nitrate transporter	PFAM	PF16974	NAR2	coord: 26..201 e-value: 1.0E-76 score: 256.2
IPR016605	High-affinity nitrate transporter	PANTHER	PTHR34806	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 1..204
None	No IPR available	PANTHER	PTHR34806:SF1	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 1..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0016877.1	Tan0016877.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042128	nitrate assimilation
biological_process	GO:0015706	nitrate transport
biological_process	GO:0010167	response to nitrate
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0015112	nitrate transmembrane transporter activity