Tan0016702 (gene) Snake gourd v1

BLAST of Tan0016702 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 5.3e-270
Identity = 451/589 (76.57%), Postives = 507/589 (86.08%), Query Frame = 0

Query: 29  LQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIPVVSYEDLKS 88
           LQ IE+VTT+AD VQRRVL EILSRNA VEYL++HGL G TDRETFK  +PVV+YED++ 
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 89  YIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 148
            I+RI+NGD S +LCS PISEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 149 VPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNETILC 208
           VPGL+KGKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPN+TILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 209 TDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTGTINPRIT 268
           +DSYQSMYSQ LCGLC  ++VLRVGAVFASGFIRAIKFLE HW  LA DIRTGT++  IT
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 269 DPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTMSQYIPTL 328
           D  VR++V  ILKPDP LAD +E ECRK +W GIITRLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 265 DSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 329 DYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRTSGFGNSS 388
           DYYSN LPLVCTMYASSECYFG+NL PLCKP EVSYTLIP MAYFEFLP+ R SG  +S 
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSI 384

Query: 389 TCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNNAPQFNFV 448
           +      EK  +ELVDLVDV+LG+EYELV+TTY+GLYRYRVGD+L V+GFKNNAPQF+F+
Sbjct: 385 SLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAPQFSFI 444

Query: 449 CRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHYVLYWEIS 508
           CRKNV+LSIDS+K DEVELQNAV  A  +L+P+D  L EYTSYADT +IPGHYVL+WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 509 LKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDKLMDYAIN 568
           L     IPP  VFE CCLTIEESLNSVYRQGRVSDKSIGPLE+K+VE+GTFDKLMDYAI+
Sbjct: 505 LNGNTPIPPS-VFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAIS 564

Query: 569 HGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRKEWSTN 618
            GASINQYKTPRCVK  PI+ELLNS VV +YFSPKCP W P  K+W +N
Sbjct: 565 LGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQWGSN 612

BLAST of Tan0016702 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 2.2e-268
Identity = 454/617 (73.58%), Postives = 515/617 (83.47%), Query Frame = 0

Query: 1   MPEASNNLPSKLPSFDTHHPHDKDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYL 60
           MPEA       L  FD     D+ +   LQ IEE+T++ADQVQR+VL EIL+RNA VEYL
Sbjct: 1   MPEAPKK--ESLEVFDL--TLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYL 60

Query: 61  QQHGLNGCTDRETFKKTIPVVSYEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGE 120
           ++H LNG TDRETFK  +PV++YED++  I+RI+NGD SPIL S+PISEFLTSSGTSGGE
Sbjct: 61  RRHDLNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGE 120

Query: 121 RKLMPTTEEELKRRSFLYSLLMPVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLT 180
           RKLMPT EEEL RRS LYSLLMPVM+QFVPGL  GKGMYFLF+KSES TPGGLPARPVLT
Sbjct: 121 RKLMPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLT 180

Query: 181 SYYKSSHFKDRPYDLYTDYTSPNETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGF 240
           SYYKSSHFK+RPYD YT+YTSPNETILC+DSYQSMYSQ LCGLC  Q+VLRVGAVFASGF
Sbjct: 181 SYYKSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGF 240

Query: 241 IRAIKFLESHWLLLANDIRTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWA 300
           IRAIKFLE HW+ L  DIRTGT++  ITDP VR++V  ILKP P LAD +EFEC+K +W 
Sbjct: 241 IRAIKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQ 300

Query: 301 GIITRLWPNTKYIDVIVTGTMSQYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPI 360
           GIITRLWPNTKY+DVIVTGTMSQYIPTLDYYSN LPLVCTMYASSECYFG+NL PLCKP 
Sbjct: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPS 360

Query: 361 EVSYTLIPTMAYFEFLPLDRTSGFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITT 420
           EVSYTLIP+MAYFEFLP+ R +G  NS        EK  +ELVDLVDV+LG+EYELV+TT
Sbjct: 361 EVSYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTT 420

Query: 421 YSGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIP 480
           Y+GL RYRVGD+LRV+GFKN APQF+F+CRKNV+LSIDS+K DEVELQNAV  A  +L+P
Sbjct: 421 YAGLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVP 480

Query: 481 YDICLLEYTSYADTHTIPGHYVLYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGR 540
           +D  L EYTSYADT +IPGHYVL+WE+ L     IPP  VFE CCL +EES N+VYRQGR
Sbjct: 481 FDASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPS-VFEDCCLAVEESFNTVYRQGR 540

Query: 541 VSDKSIGPLELKIVENGTFDKLMDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYF 600
           VSDKSIGPLE+KIVE GTFDKLMDYAI+ GASINQYKTPRCVK  PI+ELLNS VV +YF
Sbjct: 541 VSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYF 600

Query: 601 SPKCPNWVPSRKEWSTN 618
           SPKCP WVP  K+W +N
Sbjct: 601 SPKCPKWVPGHKQWGSN 612

BLAST of Tan0016702 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 2.2e-223
Identity = 379/588 (64.46%), Postives = 457/588 (77.72%), Query Frame = 0

Query: 29  LQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTD-RETFKKTIPVVSYEDLK 88
           L+ IE VT +A QVQRRVL EIL++NA  EYL+++G+ G  D  + F++ +P+V+YE L+
Sbjct: 24  LEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEGLQ 83

Query: 89  SYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQ 148
             I RI+NGD SPI   +PISEFLTSSGTSGGERKLMPT  +E+ RRS LYSLLMPVM+Q
Sbjct: 84  PDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVMSQ 143

Query: 149 FVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNETIL 208
            V GL+KGK MY LFVK+ES TPGGL ARPVLTSYY+S  F DRP D YT YTSP+E IL
Sbjct: 144 SVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEAIL 203

Query: 209 CTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTGTINPRI 268
           C DSYQSMY+Q LCGL     VLRVGAVFASGF+RAI FLE HW  L +DIRTG ++P I
Sbjct: 204 CVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDPEI 263

Query: 269 TDPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTMSQYIPT 328
           TD +VR +V  +L+ DP LAD IE EC + +W GII RLWP TKYIDVIVTGTMSQYIPT
Sbjct: 264 TDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYIPT 323

Query: 329 LDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRTSGFGNS 388
           L++Y   LPL CTMYASSECYFG+NLNP+CKP +V+YTLIPTM Y+EFLP++        
Sbjct: 324 LEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVN-------- 383

Query: 389 STCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNNAPQFNF 448
             C +   E  + +LVDLVDV+LG EYELV+TTYSGLYRYRVGD+LRV+GFKN AP F+F
Sbjct: 384 --CNNATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSF 443

Query: 449 VCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHYVLYWEI 508
           V R+NV LS+DS+K DE EL  AV  A ++L P+   L+EYTSYAD  TIPGHYVL+WE 
Sbjct: 444 VRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWE- 503

Query: 509 SLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDKLMDYAI 568
            L+  +T  P  VFE+CCL++EE+LNSVYRQGR  D+SIGPLE+++V  GTFDKLMDYAI
Sbjct: 504 -LRAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAI 563

Query: 569 NHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRKEWS 616
           + GASINQYK PRCV+  P++ELL++ V   YFSPKCP W P  K+W+
Sbjct: 564 SRGASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQWN 599

BLAST of Tan0016702 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 9.1e-222
Identity = 381/596 (63.93%), Postives = 456/596 (76.51%), Query Frame = 0

Query: 20  PHDKDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIP 79
           P  + D   L+ IEE+T +AD VQ  +L EIL+RNA  EYL++  L G TDR+TFK  IP
Sbjct: 14  PACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIP 73

Query: 80  VVSYEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 139
           V++YEDL+  I RI++GD SPIL + PISEFLTSSGTS GERKLMPT +EEL RR  LYS
Sbjct: 74  VITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYS 133

Query: 140 LLMPVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 199
           LLMPVMN +VPGL+KGKGMYFLFVKSE+ TPGGLPARPVLTSYYKS HF+ RPYD Y  Y
Sbjct: 134 LLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVY 193

Query: 200 TSPNETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIR 259
           TSPNE ILC DS+QSMY+Q LCGL     VLRVGAVFASG +RAI+FL+ HW   A+DI 
Sbjct: 194 TSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIE 253

Query: 260 TGTINPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTG 319
            G ++  ITDP +RQ +  ILKPDPVLA+ I  EC+ + W  IITR+WPNTKY+DVIVTG
Sbjct: 254 LGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTG 313

Query: 320 TMSQYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLD 379
            M+QYIPTL+YYS  LP+ CTMYASSECYFG+NLNP+ KP EVSYT++P MAYFEF+PL 
Sbjct: 314 AMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG 373

Query: 380 RTSGFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFK 439
            T                   + V+LVDV +GKEYELV+TTY+GL RYRVGDILRV+GF 
Sbjct: 374 GT-------------------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFH 433

Query: 440 NNAPQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDIC---LLEYTSYADTHT 499
           N+APQF+FV RKNV+LSIDS+K DE ELQ AV  AS   I ++ C   + EYTSYADT T
Sbjct: 434 NSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASS--ILHEECGSRVAEYTSYADTST 493

Query: 500 IPGHYVLYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVEN 559
           IPGHYVLYWE+ +++    P H    +CCL +EESLNSVYRQ RV+D S+GPLE+++V N
Sbjct: 494 IPGHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRN 553

Query: 560 GTFDKLMDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRK 613
           GTF++LMDYAI+ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P R+
Sbjct: 554 GTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

BLAST of Tan0016702 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 760.8 bits (1963), Expect = 1.2e-218
Identity = 371/589 (62.99%), Postives = 452/589 (76.74%), Query Frame = 0

Query: 25  DLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIPVVSYE 84
           D+  L+ I+E+TT+ D VQ RVL EIL RNA  EYL + GL+G TDR  F+  +PVVSY+
Sbjct: 26  DVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYD 85

Query: 85  DLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPV 144
           DL+ YI RI+NGD SPIL + P+SEFLTSSGTS GERKLMPT  +EL RR  LYSLLMPV
Sbjct: 86  DLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPV 145

Query: 145 MNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNE 204
           MN +VPGL+KGKG+YFLFVKSE+ TPGGL ARPVLTSYYKS HFK+RPYD Y +YTSP  
Sbjct: 146 MNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTA 205

Query: 205 TILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTGTIN 264
            ILC D++QSMY+Q +CGLC    VLR+GAVFASG +RAI+FL+ +W  LA+DI +G + 
Sbjct: 206 AILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELT 265

Query: 265 PRITDPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTMSQY 324
           PR+TDP VR++V  IL PDP LA LI  EC K  WAGIITR+WPNTKY+DVIVTG M+QY
Sbjct: 266 PRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQY 325

Query: 325 IPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRTSGF 384
           IPTL++YS  LP+ CTMYASSECYFG+NL P+C P EVSYT++P M YFEFLP+D T   
Sbjct: 326 IPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAA 385

Query: 385 GNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNNAPQ 444
              +T           +LVDL  VE+G+EYELVITTY+GL RYRVGD+LRV+GF N APQ
Sbjct: 386 SGDAT-----------QLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQ 445

Query: 445 FNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHYVLY 504
           F FV RKNV+LSI+S+K DE ELQ AV  AS  L P+   ++EYTS A T  IPGHYV+Y
Sbjct: 446 FRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIY 505

Query: 505 WEISLKETN-TIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDKLM 564
           WE+  K    T+       +CCL +EE+LN+VYRQ RV+D SIGPLE+++V  GTF++LM
Sbjct: 506 WELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELM 565

Query: 565 DYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRK 613
           DYAI+ GASINQYK PRCV   PI+ELL+S VVS++FSP  P+W P+R+
Sbjct: 566 DYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

BLAST of Tan0016702 vs. NCBI nr
Match: XP_022948165.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1070.5 bits (2767), Expect = 5.7e-309
Identity = 523/594 (88.05%), Postives = 549/594 (92.42%), Query Frame = 0

Query: 23  KDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIPVVS 82
           KD+ A+LQHIEE+TTHADQVQRRVL EILSRNAHVEYLQQ GL+GC DR  FK  +PVVS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 142
           YE+LK YIDRI+NGDASPILCSEP+SEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 123

Query: 143 PVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 202
           PVMNQF+PGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTGT 262
           N+TILCTDSYQSMY QFLCGLCLSQQVLRVG+VFASGFIRAIKFLE+HW+LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTMS 322
           INPRI DPLVRQSVMNILKPDPVLADLIEFEC K TWAGII RLWPNTKYIDVIVTGTMS
Sbjct: 244 INPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMS 303

Query: 323 QYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRTS 382
           QYIPTLDYYSN LPLVCTMYASSECYFG+NLNPLC P EVSYTLIPTMAYFEFLPLDR  
Sbjct: 304 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNNA 442
                         K++EELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKN A
Sbjct: 364 --------------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKA 423

Query: 443 PQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHYV 502
           PQFNFVCRKNVILSIDS+KVDEVELQNAV+EASKNLIPY+  LLEYTSYADTHT+PGHYV
Sbjct: 424 PQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYV 483

Query: 503 LYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDKL 562
           +YWEIS KETN I P LVFE CCLTIEESLNSVYRQGRVSDKSIGPLE+KI+ENGTFDKL
Sbjct: 484 IYWEISQKETNAI-PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKL 543

Query: 563 MDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRKEWST 617
           MDYAINHG+SINQYKTPRCVKH PILELLNS VVSNYFSP+CP WVP +KEWST
Sbjct: 544 MDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWST 580

BLAST of Tan0016702 vs. NCBI nr
Match: KAG6604833.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1070.1 bits (2766), Expect = 7.1e-309
Identity = 522/594 (87.88%), Postives = 550/594 (92.59%), Query Frame = 0

Query: 23  KDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIPVVS 82
           KD+ A+LQHIEE+TTHADQVQRRVL EILSRNAHVEYLQQ GL+GC DR  FK  +PVVS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 142
           YE+LK YIDRI+NGDASPILCSEP+SEFLTSSGTSGGERKLMPTT+EELKRRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTKEELKRRSFLYSLLM 123

Query: 143 PVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 202
           PVMNQF+PGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTGT 262
           N+TILCTDSYQSMY QFLCGLCLSQQVLRVG+VFASGFIRAIKFLE+HW+LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTMS 322
           INPRI+DPLVRQSVMNILKPDPVLADLIEFEC K TWAGII RLWPNTKYIDVIVTGTMS
Sbjct: 244 INPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMS 303

Query: 323 QYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRTS 382
           QYIPTLDYYSN LPLVCTMYASSECYFG+NLNPLC P EVSYTLIPTMAYFEFLPLDR  
Sbjct: 304 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNNA 442
                         K++EELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKN A
Sbjct: 364 --------------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKA 423

Query: 443 PQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHYV 502
           PQFNFVCRKNVILSIDS+KVDEVELQNAV+EASKNLIPY+  LLEYTSYADTHT+PGHYV
Sbjct: 424 PQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYV 483

Query: 503 LYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDKL 562
           +YWEIS KETN I P LVFE CCLTIEESLNSVYRQGRVSDKSIGPLE+KI+ENGTFDKL
Sbjct: 484 IYWEISQKETNAI-PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKL 543

Query: 563 MDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRKEWST 617
           MDYAINHG+SINQYKTPRCVKH PILELLNS VVSNYFSP+CP WVP +KEWST
Sbjct: 544 MDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGKKEWST 580

BLAST of Tan0016702 vs. NCBI nr
Match: XP_023533206.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.2e-308
Identity = 521/595 (87.56%), Postives = 552/595 (92.77%), Query Frame = 0

Query: 22  DKDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIPVV 81
           ++D+ AILQHIEE+TTHADQVQRRVL EILSRNAHVEYLQQ GL+GC DR  FK  +PVV
Sbjct: 10  ERDEGAILQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVV 69

Query: 82  SYEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLL 141
           SYE+LK YIDRI+NGDASPILCSEP+SEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLL
Sbjct: 70  SYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLL 129

Query: 142 MPVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTS 201
           MPVMNQF+PGLN+GKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTS
Sbjct: 130 MPVMNQFIPGLNRGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTS 189

Query: 202 PNETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTG 261
           PN+TILCTDSYQSMYSQFLCGLCLSQQVLRVG+VFASGFIRAIKFLE+HW+LLANDIRTG
Sbjct: 190 PNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTG 249

Query: 262 TINPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTM 321
           TINPRI+DPLVRQSVMNILKPDPVLADLIEFEC K TWAGII RLWPNTKYIDVIVTGTM
Sbjct: 250 TINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTM 309

Query: 322 SQYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRT 381
           SQYIPTLDYYSN LPLVCTMYASSECYFG+NLNPLC P EVSYTLIPTMAYFEFLPLDR 
Sbjct: 310 SQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR- 369

Query: 382 SGFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNN 441
                          +++EELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKN 
Sbjct: 370 ---------------EMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNK 429

Query: 442 APQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHY 501
           APQFNFVCRKNVILSIDS+KVDEVELQNAV+EASKNL+PY+  LLEYTSYADTHT+PGHY
Sbjct: 430 APQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLVPYETSLLEYTSYADTHTVPGHY 489

Query: 502 VLYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDK 561
           V+YWEIS KETN I P LVFE CCLTIEESLNSVYRQGRVSDKSIGPLELKI+ENGTFDK
Sbjct: 490 VIYWEISQKETNAI-PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDK 549

Query: 562 LMDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRKEWST 617
           LMDYAINHG+SINQYKTPRCVKH PILELLNS VVSNYFSP+CP WVP +KEWS+
Sbjct: 550 LMDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSS 587

BLAST of Tan0016702 vs. NCBI nr
Match: XP_022970970.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1066.6 bits (2757), Expect = 8.0e-308
Identity = 522/594 (87.88%), Postives = 549/594 (92.42%), Query Frame = 0

Query: 23  KDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIPVVS 82
           KD+ A+LQHIEE+TTHADQVQRRVL EILSRNAHVEYLQQ GL+GC DR  FK  +PVVS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 142
           YE+LK YIDRI+NGDASPILCSEPISEFLTSSGTSGGERKLMPTT+EELKRRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYSLLM 123

Query: 143 PVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 202
           PVMNQF+PGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTGT 262
           N+TILCTDSYQSMYSQFLCGLCLSQQVLRVG+VFASGFIRAIKFLE+HW+LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTMS 322
           INPRI+DPLVRQSVMNILKPDPVLADLIEFEC K TWAGIITRLWPNTKY+DVIVTGTMS
Sbjct: 244 INPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTGTMS 303

Query: 323 QYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRTS 382
           QYIPTLD YSN LPLVCTMYASSECYFG+NLNPLC P EVSYTLIPTMAYFEFLPLDR  
Sbjct: 304 QYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNNA 442
                         K++EELVDLVDVELGKEYEL+ITTYSGLYRYRVGDILRVSGFKN A
Sbjct: 364 --------------KMSEELVDLVDVELGKEYELIITTYSGLYRYRVGDILRVSGFKNKA 423

Query: 443 PQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHYV 502
           PQFNFVCRKNVILSIDS+KVDEVELQNAV+EASKNLIPY+  LLEYTSYADTHT+PGHYV
Sbjct: 424 PQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYV 483

Query: 503 LYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDKL 562
           +YWEIS KETN I P  VFE CCLTIEESLNSVYRQGRVSDKSIGPLELKI+ENGTFDKL
Sbjct: 484 IYWEISQKETNAI-PDWVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKL 543

Query: 563 MDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRKEWST 617
           MDYAINHG+SINQYKTPRCVK  PILELLNS VVSNYFSPKCP WVP +KEWST
Sbjct: 544 MDYAINHGSSINQYKTPRCVKREPILELLNSYVVSNYFSPKCPKWVPGQKEWST 580

BLAST of Tan0016702 vs. NCBI nr
Match: XP_022140599.1 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia])

HSP 1 Score: 1057.4 bits (2733), Expect = 4.9e-305
Identity = 520/624 (83.33%), Postives = 565/624 (90.54%), Query Frame = 0

Query: 1   MPEASNNLPSKLPSF--DTHHPHDKDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVE 60
           MPEASNNLP +  S    +H  H++     LQ+IE+VT HADQVQRRVL EILS NAHVE
Sbjct: 1   MPEASNNLPPEPDSLIGSSHEKHER----ALQYIEQVTAHADQVQRRVLTEILSANAHVE 60

Query: 61  YLQQHGLNGCTDRETFKKTIPVVSYEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSG 120
           YL +HGL+G  D +TFKKT+PVVSY+D+K Y+DRI+NGD SPILCS+PISEFLTSSGTSG
Sbjct: 61  YL-RHGLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSG 120

Query: 121 GERKLMPTTEEELKRRSFLYSLLMPVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPV 180
           GERKLMPTTEEEL+R+S LYSLLMPVMNQF+PGL+KGKGMYFLFVKSESTTPGGL ARPV
Sbjct: 121 GERKLMPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPV 180

Query: 181 LTSYYKSSHFKDRPYDLYTDYTSPNETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFAS 240
           LTSYY+S HFKDRPYD Y  YTSPNETILCTDSYQSMYSQFLCGLCLS++VLRVGAVFAS
Sbjct: 181 LTSYYRSPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFAS 240

Query: 241 GFIRAIKFLESHWLLLANDIRTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECRKET 300
           GFIRAIKFLE+HWLLL+NDIRTGTINPRITDPLVRQSV+NILKPDP LADLIE EC K T
Sbjct: 241 GFIRAIKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGT 300

Query: 301 WAGIITRLWPNTKYIDVIVTGTMSQYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCK 360
           W GII+RLW NTKYIDVIVTGTMSQYIPTLDYYSN LPLVCTMYASSECYFGINLNPLC 
Sbjct: 301 WEGIISRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCN 360

Query: 361 PIEVSYTLIPTMAYFEFLPLDRTSGFGNSSTCESEAIEKINEELVDLVDVELGKEYELVI 420
           P EVSYTLIPTMAYFEFLPLD T+GFG SSTC+SEA +K ++ELVDLVDV+LGKEYELVI
Sbjct: 361 PSEVSYTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVI 420

Query: 421 TTYSGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNL 480
           TTY+GLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDS+KVDEVELQNAV+EASKNL
Sbjct: 421 TTYAGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNL 480

Query: 481 IPYDICLLEYTSYADTHTIPGHYVLYWEISLKE----TNTIPPHLVFEKCCLTIEESLNS 540
           IPY  C++EYTSYADT TIPGHYV+YWEISLKE     N + P  VFEKCCL++EESLNS
Sbjct: 481 IPYGACVVEYTSYADTVTIPGHYVIYWEISLKEATANNNNVIPGSVFEKCCLSMEESLNS 540

Query: 541 VYRQGRVSDKSIGPLELKIVENGTFDKLMDYAINHGASINQYKTPRCVKHLPILELLNSN 600
           VYRQGRVSDKSIGPLE+KIVE GTFDKLMDYA+NHGASINQYKTPRCVKH PILELLNS 
Sbjct: 541 VYRQGRVSDKSIGPLEMKIVETGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSY 600

Query: 601 VVSNYFSPKCPNWVPSRKEWSTNH 619
           VV NYFSPKCP+WVP RKEWSTN+
Sbjct: 601 VVDNYFSPKCPHWVPGRKEWSTNN 619

BLAST of Tan0016702 vs. ExPASy TrEMBL
Match: A0A6J1G8Z2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111451824 PE=3 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 2.8e-309
Identity = 523/594 (88.05%), Postives = 549/594 (92.42%), Query Frame = 0

Query: 23  KDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIPVVS 82
           KD+ A+LQHIEE+TTHADQVQRRVL EILSRNAHVEYLQQ GL+GC DR  FK  +PVVS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 142
           YE+LK YIDRI+NGDASPILCSEP+SEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 123

Query: 143 PVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 202
           PVMNQF+PGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTGT 262
           N+TILCTDSYQSMY QFLCGLCLSQQVLRVG+VFASGFIRAIKFLE+HW+LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTMS 322
           INPRI DPLVRQSVMNILKPDPVLADLIEFEC K TWAGII RLWPNTKYIDVIVTGTMS
Sbjct: 244 INPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMS 303

Query: 323 QYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRTS 382
           QYIPTLDYYSN LPLVCTMYASSECYFG+NLNPLC P EVSYTLIPTMAYFEFLPLDR  
Sbjct: 304 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNNA 442
                         K++EELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKN A
Sbjct: 364 --------------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKA 423

Query: 443 PQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHYV 502
           PQFNFVCRKNVILSIDS+KVDEVELQNAV+EASKNLIPY+  LLEYTSYADTHT+PGHYV
Sbjct: 424 PQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYV 483

Query: 503 LYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDKL 562
           +YWEIS KETN I P LVFE CCLTIEESLNSVYRQGRVSDKSIGPLE+KI+ENGTFDKL
Sbjct: 484 IYWEISQKETNAI-PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKL 543

Query: 563 MDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRKEWST 617
           MDYAINHG+SINQYKTPRCVKH PILELLNS VVSNYFSP+CP WVP +KEWST
Sbjct: 544 MDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWST 580

BLAST of Tan0016702 vs. ExPASy TrEMBL
Match: A0A6J1I4E6 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111469778 PE=3 SV=1)

HSP 1 Score: 1066.6 bits (2757), Expect = 3.9e-308
Identity = 522/594 (87.88%), Postives = 549/594 (92.42%), Query Frame = 0

Query: 23  KDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIPVVS 82
           KD+ A+LQHIEE+TTHADQVQRRVL EILSRNAHVEYLQQ GL+GC DR  FK  +PVVS
Sbjct: 4   KDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVS 63

Query: 83  YEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLM 142
           YE+LK YIDRI+NGDASPILCSEPISEFLTSSGTSGGERKLMPTT+EELKRRSFLYSLLM
Sbjct: 64  YENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYSLLM 123

Query: 143 PVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 202
           PVMNQF+PGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP
Sbjct: 124 PVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSP 183

Query: 203 NETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTGT 262
           N+TILCTDSYQSMYSQFLCGLCLSQQVLRVG+VFASGFIRAIKFLE+HW+LLANDIRTGT
Sbjct: 184 NQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGT 243

Query: 263 INPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTMS 322
           INPRI+DPLVRQSVMNILKPDPVLADLIEFEC K TWAGIITRLWPNTKY+DVIVTGTMS
Sbjct: 244 INPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTGTMS 303

Query: 323 QYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRTS 382
           QYIPTLD YSN LPLVCTMYASSECYFG+NLNPLC P EVSYTLIPTMAYFEFLPLDR  
Sbjct: 304 QYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-- 363

Query: 383 GFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNNA 442
                         K++EELVDLVDVELGKEYEL+ITTYSGLYRYRVGDILRVSGFKN A
Sbjct: 364 --------------KMSEELVDLVDVELGKEYELIITTYSGLYRYRVGDILRVSGFKNKA 423

Query: 443 PQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHYV 502
           PQFNFVCRKNVILSIDS+KVDEVELQNAV+EASKNLIPY+  LLEYTSYADTHT+PGHYV
Sbjct: 424 PQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYV 483

Query: 503 LYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDKL 562
           +YWEIS KETN I P  VFE CCLTIEESLNSVYRQGRVSDKSIGPLELKI+ENGTFDKL
Sbjct: 484 IYWEISQKETNAI-PDWVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKL 543

Query: 563 MDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRKEWST 617
           MDYAINHG+SINQYKTPRCVK  PILELLNS VVSNYFSPKCP WVP +KEWST
Sbjct: 544 MDYAINHGSSINQYKTPRCVKREPILELLNSYVVSNYFSPKCPKWVPGQKEWST 580

BLAST of Tan0016702 vs. ExPASy TrEMBL
Match: A0A6J1CG47 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011212 PE=3 SV=1)

HSP 1 Score: 1057.4 bits (2733), Expect = 2.3e-305
Identity = 520/624 (83.33%), Postives = 565/624 (90.54%), Query Frame = 0

Query: 1   MPEASNNLPSKLPSF--DTHHPHDKDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVE 60
           MPEASNNLP +  S    +H  H++     LQ+IE+VT HADQVQRRVL EILS NAHVE
Sbjct: 1   MPEASNNLPPEPDSLIGSSHEKHER----ALQYIEQVTAHADQVQRRVLTEILSANAHVE 60

Query: 61  YLQQHGLNGCTDRETFKKTIPVVSYEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSG 120
           YL +HGL+G  D +TFKKT+PVVSY+D+K Y+DRI+NGD SPILCS+PISEFLTSSGTSG
Sbjct: 61  YL-RHGLDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSG 120

Query: 121 GERKLMPTTEEELKRRSFLYSLLMPVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPV 180
           GERKLMPTTEEEL+R+S LYSLLMPVMNQF+PGL+KGKGMYFLFVKSESTTPGGL ARPV
Sbjct: 121 GERKLMPTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPV 180

Query: 181 LTSYYKSSHFKDRPYDLYTDYTSPNETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFAS 240
           LTSYY+S HFKDRPYD Y  YTSPNETILCTDSYQSMYSQFLCGLCLS++VLRVGAVFAS
Sbjct: 181 LTSYYRSPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFAS 240

Query: 241 GFIRAIKFLESHWLLLANDIRTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECRKET 300
           GFIRAIKFLE+HWLLL+NDIRTGTINPRITDPLVRQSV+NILKPDP LADLIE EC K T
Sbjct: 241 GFIRAIKFLETHWLLLSNDIRTGTINPRITDPLVRQSVLNILKPDPELADLIESECSKGT 300

Query: 301 WAGIITRLWPNTKYIDVIVTGTMSQYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCK 360
           W GII+RLW NTKYIDVIVTGTMSQYIPTLDYYSN LPLVCTMYASSECYFGINLNPLC 
Sbjct: 301 WEGIISRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCN 360

Query: 361 PIEVSYTLIPTMAYFEFLPLDRTSGFGNSSTCESEAIEKINEELVDLVDVELGKEYELVI 420
           P EVSYTLIPTMAYFEFLPLD T+GFG SSTC+SEA +K ++ELVDLVDV+LGKEYELVI
Sbjct: 361 PSEVSYTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVI 420

Query: 421 TTYSGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNL 480
           TTY+GLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDS+KVDEVELQNAV+EASKNL
Sbjct: 421 TTYAGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNL 480

Query: 481 IPYDICLLEYTSYADTHTIPGHYVLYWEISLKE----TNTIPPHLVFEKCCLTIEESLNS 540
           IPY  C++EYTSYADT TIPGHYV+YWEISLKE     N + P  VFEKCCL++EESLNS
Sbjct: 481 IPYGACVVEYTSYADTVTIPGHYVIYWEISLKEATANNNNVIPGSVFEKCCLSMEESLNS 540

Query: 541 VYRQGRVSDKSIGPLELKIVENGTFDKLMDYAINHGASINQYKTPRCVKHLPILELLNSN 600
           VYRQGRVSDKSIGPLE+KIVE GTFDKLMDYA+NHGASINQYKTPRCVKH PILELLNS 
Sbjct: 541 VYRQGRVSDKSIGPLEMKIVETGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSY 600

Query: 601 VVSNYFSPKCPNWVPSRKEWSTNH 619
           VV NYFSPKCP+WVP RKEWSTN+
Sbjct: 601 VVDNYFSPKCPHWVPGRKEWSTNN 619

BLAST of Tan0016702 vs. ExPASy TrEMBL
Match: A0A1U7YQ53 (indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC104585725 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.3e-275
Identity = 470/618 (76.05%), Postives = 526/618 (85.11%), Query Frame = 0

Query: 1   MPEASNNLPSKLPSFDTHHPHDKDDL--AILQHIEEVTTHADQVQRRVLNEILSRNAHVE 60
           MPEA     +   + D    H++ D+    L+ IE+VTT+AD++Q+RVL EILSR+AHVE
Sbjct: 1   MPEAPKVPIAASATHDYVIDHNRPDVNQNALEFIEDVTTNADEIQKRVLAEILSRSAHVE 60

Query: 61  YLQQHGLNGCTDRETFKKTIPVVSYEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSG 120
           YLQ+HGLNG TDRETFKK +PVV+YEDL+S I+RI+NGD SPILC  PISEFLTSSGTS 
Sbjct: 61  YLQRHGLNGRTDRETFKKVMPVVTYEDLQSDINRIANGDTSPILCGLPISEFLTSSGTSA 120

Query: 121 GERKLMPTTEEELKRRSFLYSLLMPVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPV 180
           GERKLMPT EEEL+RRS LYSLLMPVM+QFVPGL+KGKGMYFLF+KSE+ TPGGL ARPV
Sbjct: 121 GERKLMPTIEEELERRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSEAKTPGGLVARPV 180

Query: 181 LTSYYKSSHFKDRPYDLYTDYTSPNETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFAS 240
           LTSYYKS HFK RPYD YT+YTSPNETILC DSYQSMYSQ LCGLC ++QVLRVGAVFAS
Sbjct: 181 LTSYYKSLHFKHRPYDPYTNYTSPNETILCPDSYQSMYSQLLCGLCQNKQVLRVGAVFAS 240

Query: 241 GFIRAIKFLESHWLLLANDIRTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECRKET 300
           GFIRAI+FLE HW LL NDIRTG INP+ITDP VR +V+ ILKPDP LAD IE EC +E 
Sbjct: 241 GFIRAIRFLEKHWSLLCNDIRTGAINPQITDPSVRDAVLRILKPDPELADFIESECTREA 300

Query: 301 WAGIITRLWPNTKYIDVIVTGTMSQYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCK 360
           W GIITRLWPNTKYIDVIVTGTMSQYIPTLDYYSN LPLVCTMYASSECYFG+NLNPLCK
Sbjct: 301 WQGIITRLWPNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGLNLNPLCK 360

Query: 361 PIEVSYTLIPTMAYFEFLPLDRTSGFGNSSTCESEAIEKINEELVDLVDVELGKEYELVI 420
           P EVSYTLIPTMAYFEFLP+ R +GF  S +      EK  ++LVDLVDV+LG+EYELV+
Sbjct: 361 PNEVSYTLIPTMAYFEFLPVHRNNGFTTSISVPKSLNEKEQQDLVDLVDVKLGQEYELVV 420

Query: 421 TTYSGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNL 480
           TTY+GLYRYRVGD+LRV+GFKN APQFNFVCRKNV+LSIDS+K DEVELQ AV +A  +L
Sbjct: 421 TTYAGLYRYRVGDVLRVAGFKNKAPQFNFVCRKNVVLSIDSDKTDEVELQAAVKDAINHL 480

Query: 481 IPYDICLLEYTSYADTHTIPGHYVLYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQ 540
           +P+D  L+EYTSYADT TIPGHYVLYWE+    T  IPP  VFE CCLTIEESLNSVYRQ
Sbjct: 481 VPFDASLIEYTSYADTTTIPGHYVLYWELRHDSTTPIPPS-VFEDCCLTIEESLNSVYRQ 540

Query: 541 GRVSDKSIGPLELKIVENGTFDKLMDYAINHGASINQYKTPRCVKHLPILELLNSNVVSN 600
           GRVSDKSIGPLE+KIVE GTFDKLMDYAI+ GASINQYKTPRCVK  PI+ELLNS V+SN
Sbjct: 541 GRVSDKSIGPLEIKIVETGTFDKLMDYAISRGASINQYKTPRCVKFAPIVELLNSRVLSN 600

Query: 601 YFSPKCPNWVPSRKEWST 617
           YFSPKCP WVP  K+W T
Sbjct: 601 YFSPKCPKWVPGHKQWCT 617

BLAST of Tan0016702 vs. ExPASy TrEMBL
Match: A0A067KS90 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_00759 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 2.1e-274
Identity = 461/618 (74.60%), Postives = 528/618 (85.44%), Query Frame = 0

Query: 1   MPEASNNLPSKLPSFDTHHPHDKDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYL 60
           MPEA  N   K   ++    + K    +L++IE+VT++AD+VQ++VL EIL+ NAHVEYL
Sbjct: 1   MPEAPKNTLIKPVDYNLVEKNKK----LLEYIEDVTSNADEVQKKVLEEILTCNAHVEYL 60

Query: 61  QQHGLNGCTDRETFKKTIPVVSYEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGE 120
           Q+HG NG TDRETFKKT+PV++YED+++ I+RI+NGD SPILCS+PISEFLTSSGTSGGE
Sbjct: 61  QRHGFNGYTDRETFKKTLPVITYEDIQTDINRIANGDTSPILCSKPISEFLTSSGTSGGE 120

Query: 121 RKLMPTTEEELKRRSFLYSLLMPVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLT 180
           RKLMPT EEEL RRS LYSLLMPVMNQFVPGL KGKGMYFLF+KSE+ TPGGL ARPVLT
Sbjct: 121 RKLMPTIEEELGRRSLLYSLLMPVMNQFVPGLEKGKGMYFLFIKSEAKTPGGLVARPVLT 180

Query: 181 SYYKSSHFKDRPYDLYTDYTSPNETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGF 240
           SYYKSSHFKDRPYD YT+YTSPNETILC DSYQSMYSQ LCGLC   +VLRVGAVFASGF
Sbjct: 181 SYYKSSHFKDRPYDPYTNYTSPNETILCPDSYQSMYSQMLCGLCQRNEVLRVGAVFASGF 240

Query: 241 IRAIKFLESHWLLLANDIRTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWA 300
           IRAI+FLE HW LL +DIRTGTINP+ITD  VR++VM ILKPDP LAD +E ECRK++W 
Sbjct: 241 IRAIRFLEKHWQLLCDDIRTGTINPQITDLSVREAVMKILKPDPKLADFLETECRKDSWQ 300

Query: 301 GIITRLWPNTKYIDVIVTGTMSQYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPI 360
           GIITR+WPNTKY+DVIVTGTMSQYIPTLDYYSN LPLVCTMYASSECYFG+NL P+CKP 
Sbjct: 301 GIITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLKPICKPS 360

Query: 361 EVSYTLIPTMAYFEFLPLDRTSGFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITT 420
           EVSYTLIPTMAYFEFLP+ R +G  NS +      EK  +ELVDLVDV+LG+EYELV+TT
Sbjct: 361 EVSYTLIPTMAYFEFLPVQRNNGVTNSISMPKSLNEKEQQELVDLVDVKLGQEYELVVTT 420

Query: 421 YSGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIP 480
           Y+GLYRYRVGD+LRV+GFKN APQFNF+CRKNV+LSIDS+K DEVELQNAV  A  +L+P
Sbjct: 421 YAGLYRYRVGDVLRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNAVNHLVP 480

Query: 481 YDICLLEYTSYADTHTIPGHYVLYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGR 540
           +D  L EYTSYADT +IPGHYVL+WE+SL  +  IPP  +FE CCLTIEESLNSVYRQGR
Sbjct: 481 FDATLAEYTSYADTTSIPGHYVLFWELSLNGSTPIPPS-IFEDCCLTIEESLNSVYRQGR 540

Query: 541 VSDKSIGPLELKIVENGTFDKLMDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYF 600
           VSDKSIGPLE+KIVE GTFDKLMDYAI+ GASINQYKTPRCVK  PI+ELLNS VVS+Y 
Sbjct: 541 VSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIVELLNSRVVSSYL 600

Query: 601 SPKCPNWVPSRKEWSTNH 619
           SPKCP W+P  K+W   +
Sbjct: 601 SPKCPKWIPGHKQWGNKN 613

BLAST of Tan0016702 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 931.4 bits (2406), Expect = 3.7e-271
Identity = 451/589 (76.57%), Postives = 507/589 (86.08%), Query Frame = 0

Query: 29  LQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIPVVSYEDLKS 88
           LQ IE+VTT+AD VQRRVL EILSRNA VEYL++HGL G TDRETFK  +PVV+YED++ 
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 89  YIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 148
            I+RI+NGD S +LCS PISEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 149 VPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNETILC 208
           VPGL+KGKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPN+TILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 209 TDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTGTINPRIT 268
           +DSYQSMYSQ LCGLC  ++VLRVGAVFASGFIRAIKFLE HW  LA DIRTGT++  IT
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 269 DPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTMSQYIPTL 328
           D  VR++V  ILKPDP LAD +E ECRK +W GIITRLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 265 DSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 329 DYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRTSGFGNSS 388
           DYYSN LPLVCTMYASSECYFG+NL PLCKP EVSYTLIP MAYFEFLP+ R SG  +S 
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSI 384

Query: 389 TCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNNAPQFNFV 448
           +      EK  +ELVDLVDV+LG+EYELV+TTY+GLYRYRVGD+L V+GFKNNAPQF+F+
Sbjct: 385 SLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAPQFSFI 444

Query: 449 CRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHYVLYWEIS 508
           CRKNV+LSIDS+K DEVELQNAV  A  +L+P+D  L EYTSYADT +IPGHYVL+WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 509 LKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDKLMDYAIN 568
           L     IPP  VFE CCLTIEESLNSVYRQGRVSDKSIGPLE+K+VE+GTFDKLMDYAI+
Sbjct: 505 LNGNTPIPPS-VFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAIS 564

Query: 569 HGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRKEWSTN 618
            GASINQYKTPRCVK  PI+ELLNS VV +YFSPKCP W P  K+W +N
Sbjct: 565 LGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQWGSN 612

BLAST of Tan0016702 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 926.0 bits (2392), Expect = 1.6e-269
Identity = 454/617 (73.58%), Postives = 515/617 (83.47%), Query Frame = 0

Query: 1   MPEASNNLPSKLPSFDTHHPHDKDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYL 60
           MPEA       L  FD     D+ +   LQ IEE+T++ADQVQR+VL EIL+RNA VEYL
Sbjct: 1   MPEAPKK--ESLEVFDL--TLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYL 60

Query: 61  QQHGLNGCTDRETFKKTIPVVSYEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGE 120
           ++H LNG TDRETFK  +PV++YED++  I+RI+NGD SPIL S+PISEFLTSSGTSGGE
Sbjct: 61  RRHDLNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGE 120

Query: 121 RKLMPTTEEELKRRSFLYSLLMPVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLT 180
           RKLMPT EEEL RRS LYSLLMPVM+QFVPGL  GKGMYFLF+KSES TPGGLPARPVLT
Sbjct: 121 RKLMPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLT 180

Query: 181 SYYKSSHFKDRPYDLYTDYTSPNETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGF 240
           SYYKSSHFK+RPYD YT+YTSPNETILC+DSYQSMYSQ LCGLC  Q+VLRVGAVFASGF
Sbjct: 181 SYYKSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGF 240

Query: 241 IRAIKFLESHWLLLANDIRTGTINPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWA 300
           IRAIKFLE HW+ L  DIRTGT++  ITDP VR++V  ILKP P LAD +EFEC+K +W 
Sbjct: 241 IRAIKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQ 300

Query: 301 GIITRLWPNTKYIDVIVTGTMSQYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPI 360
           GIITRLWPNTKY+DVIVTGTMSQYIPTLDYYSN LPLVCTMYASSECYFG+NL PLCKP 
Sbjct: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPS 360

Query: 361 EVSYTLIPTMAYFEFLPLDRTSGFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITT 420
           EVSYTLIP+MAYFEFLP+ R +G  NS        EK  +ELVDLVDV+LG+EYELV+TT
Sbjct: 361 EVSYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTT 420

Query: 421 YSGLYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIP 480
           Y+GL RYRVGD+LRV+GFKN APQF+F+CRKNV+LSIDS+K DEVELQNAV  A  +L+P
Sbjct: 421 YAGLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVP 480

Query: 481 YDICLLEYTSYADTHTIPGHYVLYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGR 540
           +D  L EYTSYADT +IPGHYVL+WE+ L     IPP  VFE CCL +EES N+VYRQGR
Sbjct: 481 FDASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPS-VFEDCCLAVEESFNTVYRQGR 540

Query: 541 VSDKSIGPLELKIVENGTFDKLMDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYF 600
           VSDKSIGPLE+KIVE GTFDKLMDYAI+ GASINQYKTPRCVK  PI+ELLNS VV +YF
Sbjct: 541 VSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYF 600

Query: 601 SPKCPNWVPSRKEWSTN 618
           SPKCP WVP  K+W +N
Sbjct: 601 SPKCPKWVPGHKQWGSN 612

BLAST of Tan0016702 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 771.2 bits (1990), Expect = 6.5e-223
Identity = 381/596 (63.93%), Postives = 456/596 (76.51%), Query Frame = 0

Query: 20  PHDKDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIP 79
           P  + D   L+ IEE+T +AD VQ  +L EIL+RNA  EYL++  L G TDR+TFK  IP
Sbjct: 14  PACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIP 73

Query: 80  VVSYEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 139
           V++YEDL+  I RI++GD SPIL + PISEFLTSSGTS GERKLMPT +EEL RR  LYS
Sbjct: 74  VITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYS 133

Query: 140 LLMPVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 199
           LLMPVMN +VPGL+KGKGMYFLFVKSE+ TPGGLPARPVLTSYYKS HF+ RPYD Y  Y
Sbjct: 134 LLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVY 193

Query: 200 TSPNETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIR 259
           TSPNE ILC DS+QSMY+Q LCGL     VLRVGAVFASG +RAI+FL+ HW   A+DI 
Sbjct: 194 TSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIE 253

Query: 260 TGTINPRITDPLVRQSVMNILKPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTG 319
            G ++  ITDP +RQ +  ILKPDPVLA+ I  EC+ + W  IITR+WPNTKY+DVIVTG
Sbjct: 254 LGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTG 313

Query: 320 TMSQYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLD 379
            M+QYIPTL+YYS  LP+ CTMYASSECYFG+NLNP+ KP EVSYT++P MAYFEF+PL 
Sbjct: 314 AMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG 373

Query: 380 RTSGFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFK 439
            T                   + V+LVDV +GKEYELV+TTY+GL RYRVGDILRV+GF 
Sbjct: 374 GT-------------------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFH 433

Query: 440 NNAPQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDIC---LLEYTSYADTHT 499
           N+APQF+FV RKNV+LSIDS+K DE ELQ AV  AS   I ++ C   + EYTSYADT T
Sbjct: 434 NSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASS--ILHEECGSRVAEYTSYADTST 493

Query: 500 IPGHYVLYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVEN 559
           IPGHYVLYWE+ +++    P H    +CCL +EESLNSVYRQ RV+D S+GPLE+++V N
Sbjct: 494 IPGHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRN 553

Query: 560 GTFDKLMDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRK 613
           GTF++LMDYAI+ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P R+
Sbjct: 554 GTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

BLAST of Tan0016702 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 765.8 bits (1976), Expect = 2.7e-221
Identity = 377/590 (63.90%), Postives = 457/590 (77.46%), Query Frame = 0

Query: 25  DLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCTDRETFKKTIPVVSYE 84
           D+  L+ IEE+T + D VQ +VL EIL+RN++ EYL++  L+G  DR+TFK  +PVV+YE
Sbjct: 20  DVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVTYE 79

Query: 85  DLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPV 144
           DLK  I RISNGD SPIL S PI+EFLTSSGTS GERKLMPT EE+L RR  LYSLLMPV
Sbjct: 80  DLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLMPV 139

Query: 145 MNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNE 204
           MN +VPGL+KGKG+YFLFVKSES T GGLPARPVLTSYYKS HFK RPYD Y  YTSPNE
Sbjct: 140 MNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSPNE 199

Query: 205 TILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLESHWLLLANDIRTGTIN 264
            ILC+DS QSMY+Q LCGL +  +VLR+GAVFASG +RAI FL+++W  LA DI TGT++
Sbjct: 200 AILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGTLS 259

Query: 265 PRITDPLVRQSVMNIL-KPDPVLADLIEFECRKETWAGIITRLWPNTKYIDVIVTGTMSQ 324
            RI DP ++  +  IL KPD  LA+ +   C +E W GIIT++WPNTKY+DVIVTG M+Q
Sbjct: 260 SRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAMAQ 319

Query: 325 YIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLIPTMAYFEFLPLDRTSG 384
           YIPTL+YYS  LP+ CTMYASSE YFGINL P+CKP EVSYT++P MAYFEFLP +   G
Sbjct: 320 YIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNH-DG 379

Query: 385 FGNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNNAP 444
            G +     E        LV+L +VE+GKEYELVITTY+GLYRYRVGDILRV+GF N+AP
Sbjct: 380 DGAAEASLDET------SLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAP 439

Query: 445 QFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLEYTSYADTHTIPGHYVL 504
           QF F+ RKNV+LS++S+K DE ELQ AV  AS+        ++EYTSYA+T TIPGHYV+
Sbjct: 440 QFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVI 499

Query: 505 YWE-ISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIGPLELKIVENGTFDKL 564
           YWE +   ++N +    V  KCCL +EESLNSVYRQ RV+DKSIGPLE+++V NGTF++L
Sbjct: 500 YWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEEL 559

Query: 565 MDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNWVPSRK 613
           MDYAI+ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W P R+
Sbjct: 560 MDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERR 602

BLAST of Tan0016702 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 750.0 bits (1935), Expect = 1.5e-216
Identity = 378/605 (62.48%), Postives = 461/605 (76.20%), Query Frame = 0

Query: 10  SKLPSFDTHHPHDKDDLAILQHIEEVTTHADQVQRRVLNEILSRNAHVEYLQQHGLNGCT 69
           S L S   H P  K D+  L+ IEE+T + D VQ++V+ EILSRN+  EYL++ GL G T
Sbjct: 5   SALRSPMMHSPSTK-DVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFT 64

Query: 70  DRETFKKTIPVVSYEDLKSYIDRISNGDASPILCSEPISEFLTSSGTSGGERKLMPTTEE 129
           DR+TFK  +PVV Y+DLK  I RI+NGD S IL S PI+EFLTSSGTS GERKLMPT +E
Sbjct: 65  DRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDE 124

Query: 130 ELKRRSFLYSLLMPVMNQFVPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFK 189
           ++ RR  LYSLLMPVMN +VPGL+KGK +YFLFVK+ES TPGGLPARPVLTSYYKS  FK
Sbjct: 125 DMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFK 184

Query: 190 DRPYDLYTDYTSPNETILCTDSYQSMYSQFLCGLCLSQQVLRVGAVFASGFIRAIKFLES 249
            RP D Y  YTSPNE ILC DS QSMY+Q LCGL +  +VLR+GAVFASG +RAI FL++
Sbjct: 185 RRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQT 244

Query: 250 HWLLLANDIRTGTINPRITDPLVRQSVMNIL-KPDPVLADLIEFECRKE-TWAGIITRLW 309
           +W  LA+DI TGT++ RI+DP +++S+  IL KPD  LAD I   C ++ +W GIIT++W
Sbjct: 245 NWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIW 304

Query: 310 PNTKYIDVIVTGTMSQYIPTLDYYSNDLPLVCTMYASSECYFGINLNPLCKPIEVSYTLI 369
           PNTKY+DVIVTG M+QYIP L+YYS  LP+ CTMYASSE YFGINL P+CKP EVSYT++
Sbjct: 305 PNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIM 364

Query: 370 PTMAYFEFLPLDRTSGFGNSSTCESEAIEKINEELVDLVDVELGKEYELVITTYSGLYRY 429
           P MAYFEFLP                 +     ELV+L DVE+GKEYELVITTY+GL RY
Sbjct: 365 PNMAYFEFLP--------------HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRY 424

Query: 430 RVGDILRVSGFKNNAPQFNFVCRKNVILSIDSEKVDEVELQNAVIEASKNLIPYDICLLE 489
           RVGDIL+V+GF N+APQF FV RKNV+LSI+S+K DE ELQ+AV  AS  L      ++E
Sbjct: 425 RVGDILQVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIE 484

Query: 490 YTSYADTHTIPGHYVLYWEISLKETNTIPPHLVFEKCCLTIEESLNSVYRQGRVSDKSIG 549
           YTSYA+T TIPGHYV+YWE+ +K+    P   V  +CCL +EESLNSVYRQ RV+DKSIG
Sbjct: 485 YTSYAETKTIPGHYVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIG 544

Query: 550 PLELKIVENGTFDKLMDYAINHGASINQYKTPRCVKHLPILELLNSNVVSNYFSPKCPNW 609
           PLE+++V+NGTF++LMDYAI+ GASINQYK PRCV   PI+ELL+S VVS +FSP  P+W
Sbjct: 545 PLEIRVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHW 594

Query: 610 VPSRK 613
            P R+
Sbjct: 605 SPERR 594