Tan0016631 (gene) Snake gourd v1

Overview
NameTan0016631
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptioncytochrome P450 CYP736A12-like
LocationLG03: 74563973 .. 74566876 (+)
RNA-Seq ExpressionTan0016631
SyntenyTan0016631
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACCATTCAATTGGGCTCTTCGTGTTCTCTCTCACTGCAATTTAGCCTTTCTTTATATATTTCCAATATTTTGATTTCGATCCAATTCATAAGCCAAGTTGTGTAGCTTTACAAATCTTAACTCCGCACAAAAATGGCTTGGTTTTGGATCATTATCCAACTGCTTGCTCTTGCAGTTGTCTTTTGGCCATGGCCGTTGAGGAACAAAAACAAGAAACGTCAAATACAGCCTCCTCGTCCAAAAGGGTTTCCAATTTTTGGTAGCCTTCATTTGTTGGGAAAGGTTCCTCACAGAGATCTTCAACGTTTGTCTCAAAGATACGGACCCATCATGCACATGAAACTAGGTGTTGTAAACACCATTGTTGTCTCTTCCCCTGAAGCTGCAGAGCTCTTTCTCAAAACCCATGATCTTGCTTTTGCAACTCGCCCACCCAATGAGGTTTTCAAGAACTTGTCTAATGGGCAAAAGGGCTTGGTATTTGGGCCTTATTGGCGCAATATGCGCAAAATGTGCACGCTCGAGTTGTTTAGCAGCCACAAAGTCAAATCTTTCCAATCAACGAGAAGGGAAGAGCTTGGCTTGTTGATTGACGATCTTCGACATCAGGCAGCTGCAAACAAGGGTGTCGTTGTAAATCTAAGTTCCAAAATCTCTTCTCTCAATGCAAACACGATCTGTCTGATGATGTTTGGGAAGAAATATGTAGACGAAGAACTTGATGAGAGGGGTTTTAAATGGGTGGTACAAGAGTCCTTAAAATTAGGAGCAGCTCCTAATTTCGCTGATTTCATTCCTTATATTGGAGCTTTTGATTTTCAAGGATTGAACCGCCGTGCAAAATCTATTAATAAAGTGTTTGATAGATTTTTCGAAAGGATTATAGATGAACATCTCGAGTCCAAAAGAGATACCAAGGATTTTGTAGATGTCATGTTGGACCTCATGAGCTCCGAAGAAAATGATTATCAGCTCGATCGATCTAGCATCAAAGCTATTATTTTGGTGAGTATTCAATCGATTCATATTCTAATTATGATGACAATATACCTAAGGAGTGTAAATGAAAGAATATTCTTTCATACACACTTAACTTTTATGTTACTCTCCCATCTTTTATATTTATGTCTATTTGATCCCTAAATTAAAAAAAACATATAATACGTTCAACTTTTTAATTTTGTGTCTAAATTAGGTCTCCAACGTTCAACTTTGTGTCTAATAAGTCTCCGAAATAATTAATTTTATTTTTTAAAAAATTAACTGGATCTATTAGATATGAAATTGAAATTTATGTCTAATTGGACTCTAAACTTTCGATTTTTTGTTGGATAGATTCATGAATTTTAAAAAATATTAGATAGGTTAAAGACCTATAAGACACAAAAATTAAAAATTCATAGACCTGTTGGACATAAGATTAAAAGTTTAAAGGCCTATTAAACAGTTTTTAAAGTTTAGAAACCAAATATACACAAATTTGTGTATGATTTTTTTGCAATATACTAGAAGTGAAATTGTCCCACATTGGAAATGTGCATAGAAGTGATGGGCTCCTAGCACTATAAAAGTAGCTCATAGCTTTGGTTTCAAACCATTACAGTTAGAAACACTTTAAGTTTTGTGCTCTACTTAAATTTCCTTATTTTGAGAGAGGAAAAAGGATGTGAGTGGTATAAAGCTTTTAAGAGAGTGTTTTTCTAATTGGGGTTGGGGAGAATTTTGTCTGATTGTAACAATTTTTCAGTTTTTTTTCGAGTTTGTAGTTTTTGTGTCTCTTATTTTGTTATTTTTTTTTAATTTCTTAGTTATTTTTACTTACGCGGTAGTAGTGGTAAGTTTTTTCTCAGCAATTTGAAAGTTAAGAAACTTATTAAACATTTTTTTTCAAGTTCGAAAATCAAATAGACACGAACATAAAAGTTGAGGGACTAAACTTGTAATTTAAGTTAGATTTATATGATACGGTAACATTTCAATGTAATTTTATAAGGAAGCATGAATCTTTTGTCTTTTAATTAACTTTTTACTTAACATATCACATTTTTAGGATATGCTTCTTGCGGGAATGGACACTTCAAGCGCAACCATTGAATGGGCACTAGCTGAGCTCATCAAACATCCACAGGTAATGAAGAAATTGCAAGAAGAATTAAAAGAGGTCGTGGGTTTGAATAGGATGGTGCAAGAATTAGACTTGGGTAACTTAAAATACTTGAACATGGTAGTTAAAGAGGTTCTGAGATTGCATCCTCCAGGTCCATTAATGGTTCCACATATGGCCGTTGAAGATTGCATAGTCAATAACTATCATATTCCTAAGCAATCACGTGTAATTGTGAACGTATGGGCAATTGGACGAGATCCGAGTGCTTGGATTGATGCAGAAATGTTCTTTCCAGAGAGGTTCATTGATACTCAAGTTGAAGTGAAAGGAAAAGATTTTCAACTGATTCCATTTGGATCTGGTCGAAGAAGTTGTCCTGCAATGCAAATGGGTCTAATTGTGGTTCATTTAGTATTAGCTCAACTTGTGCATTGTTTTGATTGGAAGCTTCCCAATAATATATTGCCAGCTGAATTGGATATGACAGAAGAATTTGGTTTGACTTGCCGAGAGCTCAAAATCTTTTGGTTACTCCTATTTATCGTCTTCAGGTCTAATCAGTGATTGATATATATAACTTGCTCGATAGCTAAAGATTTTAATAATAATATAATGGGTTTTGTCTTTTATCAACCCTAGTATAAGATTTCTATGTTGGAATTTCATATATAAGAAGACATGAACCCTAGTATGAAAATGATGTTGTGGTCATGTAGGCGGCTAGCTCCACATGTACTCAGTTGATGTATTATGCATAAAGTTTTCTAATAAAGTAAAAATAGGTATTTAATTAGGG

mRNA sequence

CACCATTCAATTGGGCTCTTCGTGTTCTCTCTCACTGCAATTTAGCCTTTCTTTATATATTTCCAATATTTTGATTTCGATCCAATTCATAAGCCAAGTTGTGTAGCTTTACAAATCTTAACTCCGCACAAAAATGGCTTGGTTTTGGATCATTATCCAACTGCTTGCTCTTGCAGTTGTCTTTTGGCCATGGCCGTTGAGGAACAAAAACAAGAAACGTCAAATACAGCCTCCTCGTCCAAAAGGGTTTCCAATTTTTGGTAGCCTTCATTTGTTGGGAAAGGTTCCTCACAGAGATCTTCAACGTTTGTCTCAAAGATACGGACCCATCATGCACATGAAACTAGGTGTTGTAAACACCATTGTTGTCTCTTCCCCTGAAGCTGCAGAGCTCTTTCTCAAAACCCATGATCTTGCTTTTGCAACTCGCCCACCCAATGAGGTTTTCAAGAACTTGTCTAATGGGCAAAAGGGCTTGGTATTTGGGCCTTATTGGCGCAATATGCGCAAAATGTGCACGCTCGAGTTGTTTAGCAGCCACAAAGTCAAATCTTTCCAATCAACGAGAAGGGAAGAGCTTGGCTTGTTGATTGACGATCTTCGACATCAGGCAGCTGCAAACAAGGGTGTCGTTGTAAATCTAAGTTCCAAAATCTCTTCTCTCAATGCAAACACGATCTGTCTGATGATGTTTGGGAAGAAATATGTAGACGAAGAACTTGATGAGAGGGGTTTTAAATGGGTGGTACAAGAGTCCTTAAAATTAGGAGCAGCTCCTAATTTCGCTGATTTCATTCCTTATATTGGAGCTTTTGATTTTCAAGGATTGAACCGCCGTGCAAAATCTATTAATAAAGTGTTTGATAGATTTTTCGAAAGGATTATAGATGAACATCTCGAGTCCAAAAGAGATACCAAGGATTTTGTAGATGTCATGTTGGACCTCATGAGCTCCGAAGAAAATGATTATCAGCTCGATCGATCTAGCATCAAAGCTATTATTTTGGATATGCTTCTTGCGGGAATGGACACTTCAAGCGCAACCATTGAATGGGCACTAGCTGAGCTCATCAAACATCCACAGGTAATGAAGAAATTGCAAGAAGAATTAAAAGAGGTCGTGGGTTTGAATAGGATGGTGCAAGAATTAGACTTGGGTAACTTAAAATACTTGAACATGGTAGTTAAAGAGGTTCTGAGATTGCATCCTCCAGGTCCATTAATGGTTCCACATATGGCCGTTGAAGATTGCATAGTCAATAACTATCATATTCCTAAGCAATCACGTGTAATTGTGAACGTATGGGCAATTGGACGAGATCCGAGTGCTTGGATTGATGCAGAAATGTTCTTTCCAGAGAGGTTCATTGATACTCAAGTTGAAGTGAAAGGAAAAGATTTTCAACTGATTCCATTTGGATCTGGTCGAAGAAGTTGTCCTGCAATGCAAATGGGTCTAATTGTGGTTCATTTAGTATTAGCTCAACTTGTGCATTGTTTTGATTGGAAGCTTCCCAATAATATATTGCCAGCTGAATTGGATATGACAGAAGAATTTGGTTTGACTTGCCGAGAGCTCAAAATCTTTTGGTTACTCCTATTTATCGTCTTCAGGTCTAATCAGTGATTGATATATATAACTTGCTCGATAGCTAAAGATTTTAATAATAATATAATGGGTTTTGTCTTTTATCAACCCTAGTATAAGATTTCTATGTTGGAATTTCATATATAAGAAGACATGAACCCTAGTATGAAAATGATGTTGTGGTCATGTAGGCGGCTAGCTCCACATGTACTCAGTTGATGTATTATGCATAAAGTTTTCTAATAAAGTAAAAATAGGTATTTAATTAGGG

Coding sequence (CDS)

ATGGCTTGGTTTTGGATCATTATCCAACTGCTTGCTCTTGCAGTTGTCTTTTGGCCATGGCCGTTGAGGAACAAAAACAAGAAACGTCAAATACAGCCTCCTCGTCCAAAAGGGTTTCCAATTTTTGGTAGCCTTCATTTGTTGGGAAAGGTTCCTCACAGAGATCTTCAACGTTTGTCTCAAAGATACGGACCCATCATGCACATGAAACTAGGTGTTGTAAACACCATTGTTGTCTCTTCCCCTGAAGCTGCAGAGCTCTTTCTCAAAACCCATGATCTTGCTTTTGCAACTCGCCCACCCAATGAGGTTTTCAAGAACTTGTCTAATGGGCAAAAGGGCTTGGTATTTGGGCCTTATTGGCGCAATATGCGCAAAATGTGCACGCTCGAGTTGTTTAGCAGCCACAAAGTCAAATCTTTCCAATCAACGAGAAGGGAAGAGCTTGGCTTGTTGATTGACGATCTTCGACATCAGGCAGCTGCAAACAAGGGTGTCGTTGTAAATCTAAGTTCCAAAATCTCTTCTCTCAATGCAAACACGATCTGTCTGATGATGTTTGGGAAGAAATATGTAGACGAAGAACTTGATGAGAGGGGTTTTAAATGGGTGGTACAAGAGTCCTTAAAATTAGGAGCAGCTCCTAATTTCGCTGATTTCATTCCTTATATTGGAGCTTTTGATTTTCAAGGATTGAACCGCCGTGCAAAATCTATTAATAAAGTGTTTGATAGATTTTTCGAAAGGATTATAGATGAACATCTCGAGTCCAAAAGAGATACCAAGGATTTTGTAGATGTCATGTTGGACCTCATGAGCTCCGAAGAAAATGATTATCAGCTCGATCGATCTAGCATCAAAGCTATTATTTTGGATATGCTTCTTGCGGGAATGGACACTTCAAGCGCAACCATTGAATGGGCACTAGCTGAGCTCATCAAACATCCACAGGTAATGAAGAAATTGCAAGAAGAATTAAAAGAGGTCGTGGGTTTGAATAGGATGGTGCAAGAATTAGACTTGGGTAACTTAAAATACTTGAACATGGTAGTTAAAGAGGTTCTGAGATTGCATCCTCCAGGTCCATTAATGGTTCCACATATGGCCGTTGAAGATTGCATAGTCAATAACTATCATATTCCTAAGCAATCACGTGTAATTGTGAACGTATGGGCAATTGGACGAGATCCGAGTGCTTGGATTGATGCAGAAATGTTCTTTCCAGAGAGGTTCATTGATACTCAAGTTGAAGTGAAAGGAAAAGATTTTCAACTGATTCCATTTGGATCTGGTCGAAGAAGTTGTCCTGCAATGCAAATGGGTCTAATTGTGGTTCATTTAGTATTAGCTCAACTTGTGCATTGTTTTGATTGGAAGCTTCCCAATAATATATTGCCAGCTGAATTGGATATGACAGAAGAATTTGGTTTGACTTGCCGAGAGCTCAAAATCTTTTGGTTACTCCTATTTATCGTCTTCAGGTCTAATCAGTGA

Protein sequence

MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLSQRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVFGPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSLNANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAKSINKVFDRFFERIIDEHLESKRDTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLLAGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVLRLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDTQVEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEFGLTCRELKIFWLLLFIVFRSNQ
Homology
BLAST of Tan0016631 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 7.9e-176
Identity = 299/488 (61.27%), Postives = 381/488 (78.07%), Query Frame = 0

Query: 1   MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLS 60
           M W W  I LLAL  +   W    KNKK+++ PP P+GFPIFGSLHLLG+ P++DL RL+
Sbjct: 1   MVWIWATIGLLALVHILQAW---WKNKKKRL-PPGPRGFPIFGSLHLLGEFPNKDLHRLA 60

Query: 61  QRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF--- 120
           ++YG IM+M+LG++ TIV+SSPEAAELFLKTHDL FA+RPP+E  K++S GQK L+F   
Sbjct: 61  RKYGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEY 120

Query: 121 GPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSL 180
           G YWR+ RKMCT+EL S+HK+ SF+S RREE+ L ++ +R  AA N+GV V+LS K+SSL
Sbjct: 121 GAYWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIR-AAANNRGVAVDLSDKVSSL 180

Query: 181 NANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAK 240
           + +  C M+ GKKY DEE DERGFK VV+E+++L +APN  D+I +I   D QG  +R K
Sbjct: 181 SVDMSCRMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMK 240

Query: 241 SINKVFDRFFERIIDEHLESK--RDTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLL 300
           S+NK FD  FE+II+EHL+      T DFVDVM+  M SEE++Y+++R  IKAI+LDML+
Sbjct: 241 SVNKAFDNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLV 300

Query: 301 AGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVL 360
           A MDTS+ TIEWAL+EL++HP+ MKK+Q+EL+ VVGL++MV+E DL  L YLNMVVKE  
Sbjct: 301 ASMDTSATTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLDYLNMVVKETF 360

Query: 361 RLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDTQ 420
           RLHP  PL++PH ++EDC VN YHIPK+SRV++NVWAIGRDP+AW DAE F PERF  + 
Sbjct: 361 RLHPVAPLLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDPNAWTDAEKFIPERFEGSS 420

Query: 421 VEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEF 480
           V+V+G  FQLIPFGSGRR CP +Q+GL VV LVLAQLVHCFDW+LPNN+LP ELDMTEEF
Sbjct: 421 VDVRGNHFQLIPFGSGRRRCPGIQLGLTVVQLVLAQLVHCFDWELPNNMLPEELDMTEEF 480

Query: 481 GLTCRELK 484
           GLT    K
Sbjct: 481 GLTVPRAK 483

BLAST of Tan0016631 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 5.8e-134
Identity = 247/489 (50.51%), Postives = 334/489 (68.30%), Query Frame = 0

Query: 7   IIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLSQRYGPI 66
           ++ +L  A+ +W  P+ +  K+    PP P+G PI GSLH LG +PHR LQ L+++YGPI
Sbjct: 8   LLFVLLGALSWWILPIISPLKRHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPI 67

Query: 67  MHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF---GPYWRN 126
           M M+LG V TIVVSSP+AAELFLKTHD  FA+RP  +  + +S G  G+ F   GP+WRN
Sbjct: 68  MSMRLGSVPTIVVSSPQAAELFLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRN 127

Query: 127 MRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSLNANTIC 186
           +RK   LEL +  K+ SF   RREELG ++  ++  +AAN+  VV+LS+K++++  N   
Sbjct: 128 IRKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANE--VVDLSAKVANIIENMTY 187

Query: 187 LMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAKSINKVF 246
            ++ G+   D+  D +G   ++ E+L L    N ADF+P++G  D QGL R+ K   K  
Sbjct: 188 RLLLGRT-KDDRYDLKG---IMNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRL 247

Query: 247 DRFFERIIDEHLESKRD---TKDFVDVMLDLMSSEENDYQ-----LDRSSIKAIILDMLL 306
           D+  E IIDEH ++  +   + DF+D ML L +   N +      +DRS IKAI++D++ 
Sbjct: 248 DKILEFIIDEHEQNSSNGNASGDFIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIIS 307

Query: 307 AGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVL 366
           A +DTS  +IEW L ELIKHP+ MKK QEE+  VVG++RMV+E DL NL+Y+ MVVKE L
Sbjct: 308 AAIDTSDTSIEWILTELIKHPRAMKKCQEEIDAVVGVDRMVEETDLPNLEYVYMVVKEGL 367

Query: 367 RLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWID-AEMFFPERFIDT 426
           RLHP  PL+ PH ++ED  +N Y IPKQSRVIVN WA+GRDP+ W + AE F PERF  +
Sbjct: 368 RLHPVAPLLGPHESMEDITINGYFIPKQSRVIVNSWALGRDPNVWSENAEEFLPERFEGS 427

Query: 427 QVEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEE 484
            V+V+G+DFQL+PFGSGRR CP MQ+GLI V LV+A+LVHCFDW LPN   P  LDMTE+
Sbjct: 428 NVDVRGRDFQLLPFGSGRRGCPGMQLGLITVQLVVARLVHCFDWNLPNGTTPDNLDMTEK 487

BLAST of Tan0016631 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 401.7 bits (1031), Expect = 1.2e-110
Identity = 204/484 (42.15%), Postives = 297/484 (61.36%), Query Frame = 0

Query: 6   IIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLSQRYGP 65
           + +  LA+A+ F+   L  K +K+   PP P   PI G+LH LG +PHR L+ L+   GP
Sbjct: 5   VSLLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGP 64

Query: 66  IMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVFGP---YWR 125
           ++ + LG + T++VS+ E AE  LKTHDL FA+RP     + +      + F P   YWR
Sbjct: 65  LILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWR 124

Query: 126 NMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSLNANTI 185
            +RK+C LEL S  +V S++S R EE+GL+++  R   + + G  VNLS  +  L++ TI
Sbjct: 125 QVRKICVLELLSIKRVNSYRSIREEEVGLMME--RISQSCSTGEAVNLSELLLLLSSGTI 184

Query: 186 CLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFD-FQGLNRRAKSINK 245
             + FGKKY  EE  +  F  +  E   L  A    D+ P     D   G++ R K  + 
Sbjct: 185 TRVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHG 244

Query: 246 VFDRFFERIIDEHLESKR-------DTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDML 305
             D F + +ID+HL S++       + KD VDV+L L         L+R+++KA+ILDM 
Sbjct: 245 ELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMF 304

Query: 306 LAGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEV 365
             G DT++ T+EWA+AELIKHP VM+K Q+E++ VVG    V+E DL  L YL +++KE 
Sbjct: 305 SGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKET 364

Query: 366 LRLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDT 425
           LRLHP  PL+VP  +  D ++  YHIP ++RV +N WAIGRDP +W +AE F PERF++ 
Sbjct: 365 LRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNN 424

Query: 426 QVEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEE 479
            V+ KG+DFQLIPFG+GRR CP +  G+  V + LA L++ F+W+LP ++   +LDM+E 
Sbjct: 425 SVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEA 484

BLAST of Tan0016631 vs. ExPASy Swiss-Prot
Match: Q9LVD2 (Cytochrome P450 71B10 OS=Arabidopsis thaliana OX=3702 GN=CYP71B10 PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.3e-104
Identity = 201/488 (41.19%), Postives = 308/488 (63.11%), Query Frame = 0

Query: 3   WFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLSQR 62
           WF  +I L+++ +V     +++  ++   QPP P G PI G+LH LG++PH+ L +LS++
Sbjct: 5   WFVSLILLISILLV----AVKHSKRRWVRQPPSPPGLPIIGNLHQLGELPHQSLCKLSKK 64

Query: 63  YGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVFG---P 122
           YGP+M +KLG V T++VS+PE A+  LK +DL   +RP  E  + LS     + F     
Sbjct: 65  YGPVMLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFSRFDD 124

Query: 123 YWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSLNA 182
           YW+ +RK+C  ELF + ++ S Q  +  E+  LID +    +A++  +VNLS    SLN 
Sbjct: 125 YWKELRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAE--SASQKTLVNLSDTFLSLNV 184

Query: 183 NTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFD--FQGLNRRAK 242
           N IC  +FG  +    L+   F+ +V E+L++  + + +DF PY+G     F GL+ R +
Sbjct: 185 NVICKAVFGVNFQGTVLNNDKFQDLVHEALEMLGSFSASDFFPYVGWIVDWFTGLHARRE 244

Query: 243 SINKVFDRFFERIIDEHLESKRDTK--DFVDVMLDLMSSEE--NDYQLDRSSIKAIILDM 302
              +  D F+E++ID HL+  R+    DFVD++L L   E      +L R+ IKAI++++
Sbjct: 245 RSVRDLDAFYEQMIDLHLQKNREESEDDFVDLLLRLEKEEAVLGYGKLTRNHIKAILMNI 304

Query: 303 LLAGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLN---RMVQELDLGNLKYLNMV 362
           LL G++TS+ T+ WA+AELI++P+VMKK+Q E++  +G N   R++   ++ +L YLNMV
Sbjct: 305 LLGGINTSAITMTWAMAELIRNPRVMKKVQSEIRAQIGKNNKTRIISLDEINHLSYLNMV 364

Query: 363 VKEVLRLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPER 422
           +KE  RLHP  PL+VP   + +  +N Y I  ++R+ VNVWAIGRDP  W D E F PER
Sbjct: 365 IKETCRLHPVAPLLVPREVISEFKINGYTIQPKTRLHVNVWAIGRDPEIWKDPEEFLPER 424

Query: 423 FIDTQVEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELD 479
           F+D  ++VKG+D++L+PFGSGRR CPA+ MG+  V   LA L++ FDWKLP  +   ++ 
Sbjct: 425 FMDCDIDVKGQDYELLPFGSGRRICPAVYMGITTVEFGLANLLYHFDWKLPEGVAVEDIY 484

BLAST of Tan0016631 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.3e-104
Identity = 201/486 (41.36%), Postives = 307/486 (63.17%), Query Frame = 0

Query: 10  LLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLSQRYGPIMHM 69
           L+A  + F+   + +++++ +  PP P  +PI G+ H +    HR L+ L+++YGPI+ +
Sbjct: 20  LIATFIFFFSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPILFL 79

Query: 70  KLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVFGPY---WRNMRK 129
           + G V T+VVSS E A+ FLKTHDL FA+RPP  V K      K + F PY   WR MRK
Sbjct: 80  RFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMRK 139

Query: 130 MCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSLNANTICLMM 189
           +C LEL +S +++SF+  R+EEL  +I  +  ++ + + + VN+S  IS+  AN +  ++
Sbjct: 140 ICVLELLTSKRIESFKHVRQEELSAMIHSIWEESESGR-IAVNVSKAISTSLANILWRIL 199

Query: 190 FGKKYVDEEL--DERGF-KWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAKSINKVF 249
             KK+ D +L  D +GF   VV+ S+ +G+  N  DFIPY+   D QG+ R  K  N  F
Sbjct: 200 ARKKFSDNDLGADGKGFADLVVEVSIAVGSL-NIGDFIPYLDCLDLQGIKRALKKANARF 259

Query: 250 DRFFERIIDEHLESKR----------DTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDM 309
           D F E++IDEH+ +              KD +DV+L++  ++    ++ R  IKAI  ++
Sbjct: 260 DAFAEKMIDEHINASTIRNGEADAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITYEL 319

Query: 310 LLAGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKE 369
             AGM+TS+  +EWA++EL++HP  MKKLQ+E++ VVG    V+E DL ++ YL+ VVKE
Sbjct: 320 FSAGMETSANVLEWAMSELLRHPHAMKKLQQEIESVVGQQGTVKESDLASIVYLHCVVKE 379

Query: 370 VLRLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAW-IDAEMFFPERFI 429
            LRL+P  PL +PH ++E   V  Y+IPK++ VI+N+WAIGRDPS W  DA  F PERF+
Sbjct: 380 TLRLYPSLPLALPHESLEAVTVGGYYIPKKTMVIMNLWAIGRDPSVWGADASEFKPERFM 439

Query: 430 DTQ---VEVKG--KDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPA 474
             +   +++ G   DF+++PFG+GRR+CP   M ++ V   LAQL+H FDW++  +  P+
Sbjct: 440 QMEENGIDLSGGQSDFRMLPFGAGRRTCPGSAMAILTVEFTLAQLLHTFDWRVEGD--PS 499

BLAST of Tan0016631 vs. NCBI nr
Match: XP_022950432.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 718.8 bits (1854), Expect = 3.3e-203
Identity = 349/483 (72.26%), Postives = 409/483 (84.68%), Query Frame = 0

Query: 1   MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLS 60
           MAW WIII + AL ++FWPW L+NK K     PP PKGFPI GSLHLLGK+PHRD+QRLS
Sbjct: 1   MAWIWIIIIMFALVLLFWPWLLKNKGKN---LPPGPKGFPILGSLHLLGKLPHRDIQRLS 60

Query: 61  QRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF--- 120
           QRYGPIMHMKLG+VNTIVVSSPEAA LFLKTHDL FA+RP NEVFK++S G++ +VF   
Sbjct: 61  QRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFSKY 120

Query: 121 GPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSL 180
           G YWRNMRK+CTLEL S+ KV  F+S R+EEL LLID+LR   AA   VVVNLSSK+ SL
Sbjct: 121 GHYWRNMRKICTLELLSNQKVNFFKSMRKEELSLLIDELRE--AAKSEVVVNLSSKVCSL 180

Query: 181 NANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAK 240
           +AN  CLM+FGKK+ D +LDERGFKWVVQE+LKL AA N  DFIP+I A D QGL +RAK
Sbjct: 181 SANMTCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAK 240

Query: 241 SINKVFDRFFERIIDEHLESK-RDTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLLA 300
            +NKV DRFFERI+DEHLESK  D+KDFVDVML +M SE  +YQ+DRSSIKAI+LD+LLA
Sbjct: 241 CVNKVCDRFFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLA 300

Query: 301 GMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVLR 360
             D+S+ TI WA+AELIKHP VMKK+Q+EL++VVGLNRMV+E DL +L YL MVVKEVLR
Sbjct: 301 ATDSSAVTIGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLSHLTYLEMVVKEVLR 360

Query: 361 LHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDTQV 420
           LHPPGPL+VPH AV+DC VNN+HIPK+SR+I+NVWAIGR+PSAW DAE FFPERFI +QV
Sbjct: 361 LHPPGPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQV 420

Query: 421 EVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEFG 480
           +VKGKDFQL+PFGSGRR CP MQ+GL+ +HL+LAQLVHCFDWKLPN +LPA+LDMTE FG
Sbjct: 421 DVKGKDFQLLPFGSGRRGCPGMQVGLVTIHLILAQLVHCFDWKLPNGMLPADLDMTEAFG 478

BLAST of Tan0016631 vs. NCBI nr
Match: XP_023545010.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 712.6 bits (1838), Expect = 2.4e-201
Identity = 349/483 (72.26%), Postives = 407/483 (84.27%), Query Frame = 0

Query: 1   MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLS 60
           MAW WIII + AL ++F PW LRNK K     PP PKGFPI GSLHLLGK+PHRD+QRLS
Sbjct: 1   MAWIWIIIIMFALVLLFRPWVLRNKGKN---LPPGPKGFPILGSLHLLGKLPHRDIQRLS 60

Query: 61  QRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF--- 120
           QRYGPIMHMKLG+VNTIVVSSPEAA LFLKTHDL +A+RP NEVFK++S G++ +VF   
Sbjct: 61  QRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVYASRPTNEVFKHMSYGERSMVFSKY 120

Query: 121 GPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSL 180
           G YWRNMRK+CTLEL S+ KV  F+S RREEL LLID+LR   AA   VVVNLSSK+ SL
Sbjct: 121 GHYWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELRE--AAKSEVVVNLSSKVCSL 180

Query: 181 NANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAK 240
           +AN  CLM+FGKK+ D ELDERGFKWVVQE+LKL AA N  DFIP+I A D QGL +RAK
Sbjct: 181 SANMTCLMIFGKKFEDRELDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAK 240

Query: 241 SINKVFDRFFERIIDEHLESK-RDTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLLA 300
            +NKV DRFFERI+DEHLESK  D+KDFVDVML +M SE  +YQ+DRSSIKAI+LD+LLA
Sbjct: 241 CVNKVCDRFFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLA 300

Query: 301 GMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVLR 360
             D+S+ TI WA+AELIKHP VMKK+Q+EL++VVGLNRMV+E DL +L YL MVVKEVLR
Sbjct: 301 ATDSSAVTIGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLCHLTYLEMVVKEVLR 360

Query: 361 LHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDTQV 420
           LHPPGPL+VPH AV+DC VNN+HIPK+SR+I+NVWAIGR+PSAW DAE FFPERFI +QV
Sbjct: 361 LHPPGPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQV 420

Query: 421 EVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEFG 480
           +VKGKDFQL+PFGSGRR CP MQ+GL+ VHL+LA+LVHCFDWKLPN +LP +LDMTE FG
Sbjct: 421 DVKGKDFQLLPFGSGRRGCPGMQVGLVTVHLILAELVHCFDWKLPNGMLPVDLDMTEAFG 478

BLAST of Tan0016631 vs. NCBI nr
Match: XP_022978479.1 (cytochrome P450 CYP736A12-like [Cucurbita maxima])

HSP 1 Score: 699.5 bits (1804), Expect = 2.1e-197
Identity = 342/483 (70.81%), Postives = 405/483 (83.85%), Query Frame = 0

Query: 1   MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLS 60
           MAW W+II + AL ++F  W L+NK K     PP PKGFPI GSLHLLGK+PHRD+QRLS
Sbjct: 1   MAWIWMIIIMFALVLLFRSWLLKNKAKN---LPPGPKGFPILGSLHLLGKLPHRDIQRLS 60

Query: 61  QRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF--- 120
           QRYGPIMHMKLG+VNTIVVSSPEAA LFLKTHDL FA+RP N+VFK++S G++ +VF   
Sbjct: 61  QRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPQNQVFKHMSYGERSMVFSKY 120

Query: 121 GPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSL 180
           G YWRNMRK+CTLEL S+ KV  F+S RREEL LLID+LR   AA   VVVNLSSK+ SL
Sbjct: 121 GHYWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELRE--AAKSEVVVNLSSKVCSL 180

Query: 181 NANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAK 240
           +AN  CLM+FGKK+ D +LDERGFKWVVQE+LKL AA N  DFIP+I A D QGL +RAK
Sbjct: 181 SANMTCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAK 240

Query: 241 SINKVFDRFFERIIDEHLESKR-DTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLLA 300
            +NKV DRFFERI+DEHLESK  D+KDFVDVML +M SE  +YQ+DRSSIKAI+LD+LLA
Sbjct: 241 CVNKVCDRFFERILDEHLESKNDDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLA 300

Query: 301 GMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVLR 360
             D+S+ TI WA+AELIKHP VMK +Q+EL++VVGLNRMV+E DL +L YL +VVKEVLR
Sbjct: 301 ATDSSAVTIGWAVAELIKHPYVMKNVQDELEKVVGLNRMVEETDLCHLTYLEIVVKEVLR 360

Query: 361 LHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDTQV 420
           LHPPGPL+VPH AV+DC VNN+HIPK+SR+I+NVWAIGR+PSAW DAE FFPERFI +QV
Sbjct: 361 LHPPGPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQV 420

Query: 421 EVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEFG 480
           +VKGKDFQL+PFGSGRR CP +Q+GL+ VHL+LAQL+HCFDWKLPN +LPA+LDM E FG
Sbjct: 421 DVKGKDFQLLPFGSGRRGCPGIQVGLVTVHLILAQLIHCFDWKLPNGMLPADLDMMEAFG 478

BLAST of Tan0016631 vs. NCBI nr
Match: XP_023544864.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 675.6 bits (1742), Expect = 3.2e-190
Identity = 325/483 (67.29%), Postives = 391/483 (80.95%), Query Frame = 0

Query: 1   MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLS 60
           MAW W+II + AL ++FW W  RNK+KK    PP PKGFPI GSLHLLGK+PHRDL RLS
Sbjct: 1   MAWIWMIIIMFALVLLFWAWMERNKSKK---LPPGPKGFPIMGSLHLLGKLPHRDLHRLS 60

Query: 61  QRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF--- 120
           Q YGPIMHMKLG+VNTIVVSSPEAAELFLKTHD+ FA+R PNEVFK++S  QK + F   
Sbjct: 61  QTYGPIMHMKLGIVNTIVVSSPEAAELFLKTHDVVFASRAPNEVFKHVSFSQKTIAFAKY 120

Query: 121 GPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSL 180
           GPYWRNMRKMC LEL S+HK    QS RR+ELG  I  LR   AA  G+VVN+SSKI SL
Sbjct: 121 GPYWRNMRKMCILELLSNHKANYLQSMRRQELGFTIHQLRD--AAKSGMVVNISSKIYSL 180

Query: 181 NANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAK 240
            AN  CLM+ G+++ D+ELDE GFKW++QE L L  A N  DFIP+I   D QG  R A+
Sbjct: 181 TANMACLMIIGRRFEDKELDEMGFKWMIQEILHLAGAFNLGDFIPFIAPLDLQGFIRCAR 240

Query: 241 SINKVFDRFFERIIDEHLES-KRDTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLLA 300
           S++KV D FFE+I+DEHLES   DTKD VDVML +M S+  +YQ+D S+IKAIILDMLL 
Sbjct: 241 SVHKVCDTFFEKILDEHLESNNNDTKDLVDVMLGIMGSQATEYQIDPSTIKAIILDMLLG 300

Query: 301 GMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVLR 360
           G++TS+ TIEWALAEL++HPQ MKK+Q+EL++VVGLNRMVQE DL +L+YL MVVKE  R
Sbjct: 301 GVETSATTIEWALAELVRHPQTMKKVQDELQKVVGLNRMVQEADLDHLEYLKMVVKETFR 360

Query: 361 LHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDTQV 420
           LHPP PL++P  +V+D ++NN++IP++SR+I+NVWAIGRDPS WIDAE FFPERFI TQV
Sbjct: 361 LHPPNPLLIPRESVKDSVINNFYIPQKSRIILNVWAIGRDPSVWIDAEKFFPERFIGTQV 420

Query: 421 EVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEFG 480
           +VKG+DFQL+PFGSGRR CP +QMGL+ VHL+LAQLVHCFDWKLPN +LP ELDMTE+FG
Sbjct: 421 DVKGRDFQLLPFGSGRRGCPGIQMGLVTVHLILAQLVHCFDWKLPNGMLPDELDMTEKFG 478

BLAST of Tan0016631 vs. NCBI nr
Match: XP_022133318.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 673.3 bits (1736), Expect = 1.6e-189
Identity = 330/485 (68.04%), Postives = 398/485 (82.06%), Query Frame = 0

Query: 1   MAWFWIIIQLLALA-VVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRL 60
           MAW  + + L  LA ++  PW  R++NK     PP P+GFP+FGSLHLLGK+PHRDL+ L
Sbjct: 1   MAWILLAVALAGLALLILKPWLFRSQNK----LPPGPRGFPVFGSLHLLGKLPHRDLRDL 60

Query: 61  SQRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF-- 120
           SQ+YG IM+M+LG+V TI+V+SP+AAELFLKTHDL FA+RP  E  K +S GQK L F  
Sbjct: 61  SQKYGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQ 120

Query: 121 -GPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISS 180
            G YWRNMRKMCTLEL S HKV SF+S RREE+GLL+  LR   AA   VV+NLSSKI+S
Sbjct: 121 YGSYWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRD--AAKDHVVINLSSKIAS 180

Query: 181 LNANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRA 240
           LN +  CLM+FGKKY D E DERGFK V+QE+++L A PN  DFIP I A D QGLNRR+
Sbjct: 181 LNTDMTCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRS 240

Query: 241 KSINKVFDRFFERIIDEHLESKRD--TKDFVDVMLDLMSSEENDYQLDRSSIKAIILDML 300
           K+I+K+FD FFE+IIDEHLESK +  TKDFVDVMLD+M S+E +YQ+DRSSIKAIILDML
Sbjct: 241 KAISKIFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDML 300

Query: 301 LAGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEV 360
           +A MDTS+ T+ WA+AELIKHPQVMKKLQEEL++VVGL+R V+E DL +L+YL+M VKE+
Sbjct: 301 VAAMDTSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEI 360

Query: 361 LRLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDT 420
           LR+HP  PL+VPH ++EDC VN YHIPK+SR+IVN WAIG+DP++W+D E FFPERFI +
Sbjct: 361 LRVHPAAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGS 420

Query: 421 QVEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEE 480
           QV+VKG+DFQLIPFGSGRR CP M MGL+VVH V+AQLVHCFDWKLPN +LPAELDMTEE
Sbjct: 421 QVDVKGRDFQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEE 479

BLAST of Tan0016631 vs. ExPASy TrEMBL
Match: A0A6J1GET0 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=3 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 1.6e-203
Identity = 349/483 (72.26%), Postives = 409/483 (84.68%), Query Frame = 0

Query: 1   MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLS 60
           MAW WIII + AL ++FWPW L+NK K     PP PKGFPI GSLHLLGK+PHRD+QRLS
Sbjct: 1   MAWIWIIIIMFALVLLFWPWLLKNKGKN---LPPGPKGFPILGSLHLLGKLPHRDIQRLS 60

Query: 61  QRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF--- 120
           QRYGPIMHMKLG+VNTIVVSSPEAA LFLKTHDL FA+RP NEVFK++S G++ +VF   
Sbjct: 61  QRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFSKY 120

Query: 121 GPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSL 180
           G YWRNMRK+CTLEL S+ KV  F+S R+EEL LLID+LR   AA   VVVNLSSK+ SL
Sbjct: 121 GHYWRNMRKICTLELLSNQKVNFFKSMRKEELSLLIDELRE--AAKSEVVVNLSSKVCSL 180

Query: 181 NANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAK 240
           +AN  CLM+FGKK+ D +LDERGFKWVVQE+LKL AA N  DFIP+I A D QGL +RAK
Sbjct: 181 SANMTCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAK 240

Query: 241 SINKVFDRFFERIIDEHLESK-RDTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLLA 300
            +NKV DRFFERI+DEHLESK  D+KDFVDVML +M SE  +YQ+DRSSIKAI+LD+LLA
Sbjct: 241 CVNKVCDRFFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLA 300

Query: 301 GMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVLR 360
             D+S+ TI WA+AELIKHP VMKK+Q+EL++VVGLNRMV+E DL +L YL MVVKEVLR
Sbjct: 301 ATDSSAVTIGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLSHLTYLEMVVKEVLR 360

Query: 361 LHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDTQV 420
           LHPPGPL+VPH AV+DC VNN+HIPK+SR+I+NVWAIGR+PSAW DAE FFPERFI +QV
Sbjct: 361 LHPPGPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQV 420

Query: 421 EVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEFG 480
           +VKGKDFQL+PFGSGRR CP MQ+GL+ +HL+LAQLVHCFDWKLPN +LPA+LDMTE FG
Sbjct: 421 DVKGKDFQLLPFGSGRRGCPGMQVGLVTIHLILAQLVHCFDWKLPNGMLPADLDMTEAFG 478

BLAST of Tan0016631 vs. ExPASy TrEMBL
Match: A0A6J1IU54 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478446 PE=3 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 1.0e-197
Identity = 342/483 (70.81%), Postives = 405/483 (83.85%), Query Frame = 0

Query: 1   MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLS 60
           MAW W+II + AL ++F  W L+NK K     PP PKGFPI GSLHLLGK+PHRD+QRLS
Sbjct: 1   MAWIWMIIIMFALVLLFRSWLLKNKAKN---LPPGPKGFPILGSLHLLGKLPHRDIQRLS 60

Query: 61  QRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF--- 120
           QRYGPIMHMKLG+VNTIVVSSPEAA LFLKTHDL FA+RP N+VFK++S G++ +VF   
Sbjct: 61  QRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPQNQVFKHMSYGERSMVFSKY 120

Query: 121 GPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSL 180
           G YWRNMRK+CTLEL S+ KV  F+S RREEL LLID+LR   AA   VVVNLSSK+ SL
Sbjct: 121 GHYWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELRE--AAKSEVVVNLSSKVCSL 180

Query: 181 NANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAK 240
           +AN  CLM+FGKK+ D +LDERGFKWVVQE+LKL AA N  DFIP+I A D QGL +RAK
Sbjct: 181 SANMTCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAK 240

Query: 241 SINKVFDRFFERIIDEHLESKR-DTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLLA 300
            +NKV DRFFERI+DEHLESK  D+KDFVDVML +M SE  +YQ+DRSSIKAI+LD+LLA
Sbjct: 241 CVNKVCDRFFERILDEHLESKNDDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLA 300

Query: 301 GMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVLR 360
             D+S+ TI WA+AELIKHP VMK +Q+EL++VVGLNRMV+E DL +L YL +VVKEVLR
Sbjct: 301 ATDSSAVTIGWAVAELIKHPYVMKNVQDELEKVVGLNRMVEETDLCHLTYLEIVVKEVLR 360

Query: 361 LHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDTQV 420
           LHPPGPL+VPH AV+DC VNN+HIPK+SR+I+NVWAIGR+PSAW DAE FFPERFI +QV
Sbjct: 361 LHPPGPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQV 420

Query: 421 EVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEFG 480
           +VKGKDFQL+PFGSGRR CP +Q+GL+ VHL+LAQL+HCFDWKLPN +LPA+LDM E FG
Sbjct: 421 DVKGKDFQLLPFGSGRRGCPGIQVGLVTVHLILAQLIHCFDWKLPNGMLPADLDMMEAFG 478

BLAST of Tan0016631 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 7.7e-190
Identity = 330/485 (68.04%), Postives = 398/485 (82.06%), Query Frame = 0

Query: 1   MAWFWIIIQLLALA-VVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRL 60
           MAW  + + L  LA ++  PW  R++NK     PP P+GFP+FGSLHLLGK+PHRDL+ L
Sbjct: 1   MAWILLAVALAGLALLILKPWLFRSQNK----LPPGPRGFPVFGSLHLLGKLPHRDLRDL 60

Query: 61  SQRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF-- 120
           SQ+YG IM+M+LG+V TI+V+SP+AAELFLKTHDL FA+RP  E  K +S GQK L F  
Sbjct: 61  SQKYGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQ 120

Query: 121 -GPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISS 180
            G YWRNMRKMCTLEL S HKV SF+S RREE+GLL+  LR   AA   VV+NLSSKI+S
Sbjct: 121 YGSYWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRD--AAKDHVVINLSSKIAS 180

Query: 181 LNANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRA 240
           LN +  CLM+FGKKY D E DERGFK V+QE+++L A PN  DFIP I A D QGLNRR+
Sbjct: 181 LNTDMTCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRS 240

Query: 241 KSINKVFDRFFERIIDEHLESKRD--TKDFVDVMLDLMSSEENDYQLDRSSIKAIILDML 300
           K+I+K+FD FFE+IIDEHLESK +  TKDFVDVMLD+M S+E +YQ+DRSSIKAIILDML
Sbjct: 241 KAISKIFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDML 300

Query: 301 LAGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEV 360
           +A MDTS+ T+ WA+AELIKHPQVMKKLQEEL++VVGL+R V+E DL +L+YL+M VKE+
Sbjct: 301 VAAMDTSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEI 360

Query: 361 LRLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDT 420
           LR+HP  PL+VPH ++EDC VN YHIPK+SR+IVN WAIG+DP++W+D E FFPERFI +
Sbjct: 361 LRVHPAAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGS 420

Query: 421 QVEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEE 480
           QV+VKG+DFQLIPFGSGRR CP M MGL+VVH V+AQLVHCFDWKLPN +LPAELDMTEE
Sbjct: 421 QVDVKGRDFQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEE 479

BLAST of Tan0016631 vs. ExPASy TrEMBL
Match: A0A6J1GET5 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453534 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 2.1e-187
Identity = 322/483 (66.67%), Postives = 388/483 (80.33%), Query Frame = 0

Query: 1   MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLS 60
           MAW W+II + AL ++FW W  RNK+KK    PP PKGFPI GSLHLLGK+PHRDL RLS
Sbjct: 1   MAWIWMIIIMFALVLLFWAWMERNKSKK---LPPGPKGFPIMGSLHLLGKLPHRDLHRLS 60

Query: 61  QRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVF--- 120
           Q YGPIMHMKLG+VNTIVVSSPEAAELFLKTHD+ FA+R PNEVFK++S  QK + F   
Sbjct: 61  QTYGPIMHMKLGLVNTIVVSSPEAAELFLKTHDVVFASRAPNEVFKHVSFSQKTIAFAKY 120

Query: 121 GPYWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSL 180
           GPYWRNMRKMC LEL S+HK    QS RR+ELG  I  LR   AAN G+VVN+SSKI SL
Sbjct: 121 GPYWRNMRKMCILELLSNHKANYLQSMRRQELGFTIHQLRD--AANSGMVVNISSKIYSL 180

Query: 181 NANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAK 240
            AN  CLM+ G+++ D+ELDE GFKW++QE   L  A N  DFIP+I   D QG  R A+
Sbjct: 181 TANMACLMILGRRFEDKELDEMGFKWMIQEIFHLAGAFNLGDFIPFIAPLDLQGFIRGAR 240

Query: 241 SINKVFDRFFERIIDEHLES-KRDTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLLA 300
           S++KV D FFE+I+DEHLES   DTKD VDVML +M S+  +YQ+D S+IKAIILDMLL 
Sbjct: 241 SVHKVCDTFFEKILDEHLESNNNDTKDLVDVMLGIMGSQTTEYQIDPSTIKAIILDMLLG 300

Query: 301 GMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVLR 360
           G++T + TIEWALAELI+HPQ MKK+Q+EL+++VGLNRMVQE DL +L+YL+MVVKE  R
Sbjct: 301 GVETPATTIEWALAELIRHPQTMKKVQDELQKIVGLNRMVQEADLDHLEYLDMVVKETFR 360

Query: 361 LHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDTQV 420
           LHPP PL +P  +VED +V +++IPK+SR+I+NVWAIGRDPS WIDAE FFPERFI  QV
Sbjct: 361 LHPPSPLPIPRESVEDSVVKDFYIPKKSRIIINVWAIGRDPSVWIDAEKFFPERFIGNQV 420

Query: 421 EVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEFG 480
           +VKG+DFQL+PFGSGRR CP +QMGL+ VHLVLAQLVHCFDWKL N+++P +LDMTE+FG
Sbjct: 421 DVKGRDFQLLPFGSGRRGCPGIQMGLVTVHLVLAQLVHCFDWKLLNSMVPDKLDMTEKFG 478

BLAST of Tan0016631 vs. ExPASy TrEMBL
Match: A0A6J1BWD4 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005329 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.7e-187
Identity = 324/484 (66.94%), Postives = 388/484 (80.17%), Query Frame = 0

Query: 1   MAWFWIII-QLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRL 60
           M   WIII  LLALA++F PW LRN +KK    PP PKGFPI G LHLLGK+PHRD  RL
Sbjct: 1   MGLIWIIIVPLLALALLFRPWLLRNSHKK---LPPGPKGFPILGCLHLLGKLPHRDFHRL 60

Query: 61  SQRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVFGP 120
           SQRYGPIMHMKLG+V+TI+VSSPEAAELFLKTHDL FA+RP +EV   + +GQK ++F P
Sbjct: 61  SQRYGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAP 120

Query: 121 ---YWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISS 180
              YWRNMRK CTLEL SSHK+ SF+S RREE+GLLI+DLR   AA  GV V+L+SKI S
Sbjct: 121 YGSYWRNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLRE--AAKSGVAVDLTSKIFS 180

Query: 181 LNANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRA 240
           L     CL++FG+KY  EE+  R FKW+++E+ +LGAAPN ADFIP I   D QGLNRRA
Sbjct: 181 LTLEMTCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRA 240

Query: 241 KSINKVFDRFFERIIDEHL-ESKRDTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLL 300
           K +NKV D F E IIDEHL ESK  TKDFVD MLD++ S   +YQ++RS IKA+I DM+L
Sbjct: 241 KFVNKVLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVL 300

Query: 301 AGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVL 360
             +DTS+ T+ W L ELIKHPQVMKKLQEEL+ VVGLNR V+E DLG++KYL+M+VKE L
Sbjct: 301 GAVDTSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETL 360

Query: 361 RLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFIDTQ 420
           RLHPPGP++ PH ++EDC +NN+ IPK+SR+IVN WAI RDPS W +AE FFPERFID++
Sbjct: 361 RLHPPGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSK 420

Query: 421 VEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEF 480
           V++KG+DFQLIPFG+GRRSCP + M LIVVHL++AQLVHCFDWKLP  ILP ELD+TEEF
Sbjct: 421 VDLKGRDFQLIPFGAGRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEF 479

BLAST of Tan0016631 vs. TAIR 10
Match: AT5G57260.1 (cytochrome P450, family 71, subfamily B, polypeptide 10 )

HSP 1 Score: 381.7 bits (979), Expect = 8.9e-106
Identity = 201/488 (41.19%), Postives = 308/488 (63.11%), Query Frame = 0

Query: 3   WFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLSQR 62
           WF  +I L+++ +V     +++  ++   QPP P G PI G+LH LG++PH+ L +LS++
Sbjct: 5   WFVSLILLISILLV----AVKHSKRRWVRQPPSPPGLPIIGNLHQLGELPHQSLCKLSKK 64

Query: 63  YGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVFG---P 122
           YGP+M +KLG V T++VS+PE A+  LK +DL   +RP  E  + LS     + F     
Sbjct: 65  YGPVMLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFSRFDD 124

Query: 123 YWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSLNA 182
           YW+ +RK+C  ELF + ++ S Q  +  E+  LID +    +A++  +VNLS    SLN 
Sbjct: 125 YWKELRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAE--SASQKTLVNLSDTFLSLNV 184

Query: 183 NTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFD--FQGLNRRAK 242
           N IC  +FG  +    L+   F+ +V E+L++  + + +DF PY+G     F GL+ R +
Sbjct: 185 NVICKAVFGVNFQGTVLNNDKFQDLVHEALEMLGSFSASDFFPYVGWIVDWFTGLHARRE 244

Query: 243 SINKVFDRFFERIIDEHLESKRDTK--DFVDVMLDLMSSEE--NDYQLDRSSIKAIILDM 302
              +  D F+E++ID HL+  R+    DFVD++L L   E      +L R+ IKAI++++
Sbjct: 245 RSVRDLDAFYEQMIDLHLQKNREESEDDFVDLLLRLEKEEAVLGYGKLTRNHIKAILMNI 304

Query: 303 LLAGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLN---RMVQELDLGNLKYLNMV 362
           LL G++TS+ T+ WA+AELI++P+VMKK+Q E++  +G N   R++   ++ +L YLNMV
Sbjct: 305 LLGGINTSAITMTWAMAELIRNPRVMKKVQSEIRAQIGKNNKTRIISLDEINHLSYLNMV 364

Query: 363 VKEVLRLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPER 422
           +KE  RLHP  PL+VP   + +  +N Y I  ++R+ VNVWAIGRDP  W D E F PER
Sbjct: 365 IKETCRLHPVAPLLVPREVISEFKINGYTIQPKTRLHVNVWAIGRDPEIWKDPEEFLPER 424

Query: 423 FIDTQVEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELD 479
           F+D  ++VKG+D++L+PFGSGRR CPA+ MG+  V   LA L++ FDWKLP  +   ++ 
Sbjct: 425 FMDCDIDVKGQDYELLPFGSGRRICPAVYMGITTVEFGLANLLYHFDWKLPEGVAVEDIY 484

BLAST of Tan0016631 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 377.9 bits (969), Expect = 1.3e-104
Identity = 201/487 (41.27%), Postives = 300/487 (61.60%), Query Frame = 0

Query: 1   MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLS 60
           MA  W++  +  + ++   +  +   K RQ   P P GFPI G+LH +G++PH+ L +LS
Sbjct: 1   MAHIWLLPLIFLVCILLAVFNHKKHPKYRQF--PCPPGFPIIGNLHQIGELPHQTLWKLS 60

Query: 61  QRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVFGP- 120
           ++YGP+MH+ LG V T+VVSS + A   L+ HDL   TRP     + LS     + F P 
Sbjct: 61  KKYGPVMHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPY 120

Query: 121 --YWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSL 180
             YW+ +RK+C  ELFS+ +V S Q  + EE+  +ID +   A+      VNL++K   L
Sbjct: 121 DDYWKEVRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKN--PVNLNNKCLEL 180

Query: 181 NANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAF--DFQGLNRR 240
             + +C   FG  +    L+   F  +V+E+L++  + + ADFIPY+G       GL  R
Sbjct: 181 TVSVVCRTAFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGR 240

Query: 241 AKSINKVFDRFFERIIDEHLESKRD-TKDFVDVMLDLMSSEE---NDYQLDRSSIKAIIL 300
            +   +  + FFE++ D H E K++  +DFVD++L L   E    ND +L R+ IKAI+L
Sbjct: 241 RERSKRDLNAFFEQMFDLHKEGKKEGNEDFVDLLLRLEKEEAVLGND-KLTRNHIKAILL 300

Query: 301 DMLLAGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVV 360
           D+LLAG+DTS+ T+ WA+ EL ++P+VMKK+Q E++  +G   M+   D+  L+YL MV+
Sbjct: 301 DVLLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVI 360

Query: 361 KEVLRLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERF 420
           KE  RLHP  PL++P  A+ +  +N Y IP ++R+ VNVWAIGRDP  W D E+F PERF
Sbjct: 361 KETWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERF 420

Query: 421 IDTQVEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDM 479
           +D  ++ KG+ F+L+PFG GRR CPA+ MG  +V   LA L++ FDWKLP  +   ++D+
Sbjct: 421 MDNNIDAKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDV 480

BLAST of Tan0016631 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 369.0 bits (946), Expect = 6.0e-102
Identity = 198/487 (40.66%), Postives = 297/487 (60.99%), Query Frame = 0

Query: 1   MAWFWIIIQLLALAVVFWPWPLRNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLS 60
           M   W++  +  + ++   +  +N+ +  Q  PP P G PI G+LH LG++PH+ L +LS
Sbjct: 1   MTNIWLLSLIFVICILVAVFNHKNR-RNYQRTPPSPPGCPIIGNLHQLGELPHQSLWKLS 60

Query: 61  QRYGPIMHMKLGVVNTIVVSSPEAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVFGP- 120
           ++YGP+M +KLG V T++VSS E A+  LK HDL   +RP     + LS     + F P 
Sbjct: 61  KKYGPVMLLKLGRVPTVIVSSSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPY 120

Query: 121 --YWRNMRKMCTLELFSSHKVKSFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSL 180
             YW+ +RK+   ELFSS +V S Q  + EE+  LID +   AA  +   +NL+  + +L
Sbjct: 121 DDYWKEVRKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISESAA--QKTPINLNKTLLAL 180

Query: 181 NANTICLMMFGKKYVDEELDERGFKWVVQESLKLGAAPNFADFIPYIGAFD--FQGLNRR 240
             + +C   F   +    L+   F  +V+E+L++  + + +DFIPY+G       GL  R
Sbjct: 181 TVSVVCRTAFSVNFEGTVLNSERFNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGR 240

Query: 241 AKSINKVFDRFFERIIDEHLESKRD-TKDFVDVMLDLMSSEE---NDYQLDRSSIKAIIL 300
            +   +  D F+E++ D H + K + ++DFVD++L L   E    ND +L R+ IKAI++
Sbjct: 241 RERSMRDLDAFYEQMFDLHKQKKEEGSEDFVDLLLRLEKEEAVLGND-KLTRNHIKAILM 300

Query: 301 DMLLAGMDTSSATIEWALAELIKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVV 360
           D+LLAGMDTS+ T+ WA+AEL K+P+VMKK+Q E++  +     +   D   L+YL MV+
Sbjct: 301 DVLLAGMDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVI 360

Query: 361 KEVLRLHPPGPLMVPHMAVEDCIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERF 420
           KE  RLHP  PL++P  A+ +  +N Y IP ++R+ VNVWAIGRDP  W D E+F PERF
Sbjct: 361 KETWRLHPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERF 420

Query: 421 IDTQVEVKGKDFQLIPFGSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDM 479
            D  ++ KG+ F+L+PFG GRR CPA+ MG  +V   LA L++ FDWKLP  +   ++DM
Sbjct: 421 TDNNIDAKGQHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDM 480

BLAST of Tan0016631 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 366.7 bits (940), Expect = 3.0e-101
Identity = 197/470 (41.91%), Postives = 283/470 (60.21%), Query Frame = 0

Query: 23  RNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLSQRYGPIMHMKLGVVNTIVVSSP 82
           RN++   ++ PP P  +PI G+L  +G  PHR L  +   YGPI+H++LG V+ +V +S 
Sbjct: 25  RNRSHNNRL-PPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYGPILHLRLGFVDVVVAASK 84

Query: 83  EAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLVFGPY---WRNMRKMCTLELFSSHKVK 142
             AE FLK HD  FA+RPPN   K+++   + LVF PY   WR +RK+ ++ LFS+  ++
Sbjct: 85  SVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLRKISSVHLFSAKALE 144

Query: 143 SFQSTRREELGLLIDDLRHQAA--ANKGVVVNLSSKISSLNANTICLMMFGKKYVDEELD 202
            F+  R+EE+G L  +L        N G +VN+   +++L    I   +FG    D + D
Sbjct: 145 DFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNM-CVVNALGREMIGRRLFGAD-ADHKAD 204

Query: 203 ERGFKWVVQESLKLGAAPNFADFIPYIGAFDFQGLNRRAKSINKVFDRFFERIIDEHLES 262
           E  F+ +V E + L    N  DF+P +   D QG+  + K ++K FD F   I+ EH  +
Sbjct: 205 E--FRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRFDAFLSSILKEHEMN 264

Query: 263 KRDTKDFVDVMLDLMSSEENDYQLDRSS-----IKAIILDMLLAGMDTSSATIEWALAEL 322
            +D K   D++  L+S +  D   D  S     IKA++L+M  AG DTS++T++WA+AEL
Sbjct: 265 GQDQK-HTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFTAGTDTSASTVDWAIAEL 324

Query: 323 IKHPQVMKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVLRLHPPGPLMVPHMAVED 382
           I+HP +M K QEEL  VVG +R V E D+  L YL  V+KE  RLHPP PL +PH+A E 
Sbjct: 325 IRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASES 384

Query: 383 CIVNNYHIPKQSRVIVNVWAIGRDPSAWIDAEMFFPERFI----DTQVEVKGKDFQLIPF 442
           C +N YHIPK S ++ N+WAI RDP  W D   F PERF+     + V+VKG DF+LIPF
Sbjct: 385 CEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSGVDVKGSDFELIPF 444

Query: 443 GSGRRSCPAMQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEFGLT 479
           G+GRR C  + +GL  +  + A LV  FDW+L   + P +L+M E +GLT
Sbjct: 445 GAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNMEESYGLT 488

BLAST of Tan0016631 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 366.3 bits (939), Expect = 3.9e-101
Identity = 182/465 (39.14%), Postives = 288/465 (61.94%), Query Frame = 0

Query: 23  RNKNKKRQIQPPRPKGFPIFGSLHLLGKVPHRDLQRLSQRYGPIMHMKLGVVNTIVVSSP 82
           + K  K++   P P G P+ G+LH LG+ PHR L  LS RYGP+M +  G V  +VVSS 
Sbjct: 22  KQKRGKKRNTLPSPPGLPLIGNLHQLGRHPHRSLCSLSHRYGPLMLLHFGRVPVLVVSSA 81

Query: 83  EAAELFLKTHDLAFATRPPNEVFKNLSNGQKGLV---FGPYWRNMRKMCTLELFSSHKVK 142
           E A   LKTHD  FA+RP +++F+ L   +  +    +G YWR M+ +C L LFS+  V+
Sbjct: 82  ELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGEYWRQMKSVCVLHLFSNKMVR 141

Query: 143 SFQSTRREELGLLIDDLRHQAAANKGVVVNLSSKISSLNANTICLMMFGKKYVDEELDER 202
           SF+  R EE+ L+++ +R   +    + VNLS  + SL  + IC +  G+KY      E 
Sbjct: 142 SFREVREEEISLMMEKIRKSIS----LPVNLSKILVSLTNDVICKVALGRKYG----GET 201

Query: 203 GFKWVVQESLKLGAAPNFADFIPYIGAFDF-QGLNRRAKSINKVFDRFFERIIDEHLESK 262
            FK +++   KL    +   ++P++   D+ +GL+ + +      D+FFER++ +H++  
Sbjct: 202 DFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVDKFFERVVQDHVDGN 261

Query: 263 RDTKDFVDVMLDLMSSEENDYQLDRSSIKAIILDMLLAGMDTSSATIEWALAELIKHPQV 322
           RD  DFVDV+L +   +   ++++R SIKAI++++ + G DTSS  +EWA+ EL++HP+ 
Sbjct: 262 RDMTDFVDVLLAIQRDKTVGFEINRVSIKAIVMNVFVGGTDTSSTLMEWAMTELLRHPKC 321

Query: 323 MKKLQEELKEVVGLNRMVQELDLGNLKYLNMVVKEVLRLHPPGPLMVPHMAVEDCIVNNY 382
           +K+LQEE++ +      V E ++ N+ YL  V+KE LRLHPP PLMVPH + +D  + ++
Sbjct: 322 LKRLQEEVRTICKDKSSVSEEEIQNMSYLKAVIKEALRLHPPLPLMVPHESTQDVRLGDH 381

Query: 383 HIPKQSRVIVNVWAIGRDPSAW-IDAEMFFPERFIDTQVEVKGKDFQLIPFGSGRRSCPA 442
           HIP  ++V++N WAIGR+ + W  D E F PER +D+ V+ +G+ F+LIPFGSGRR CPA
Sbjct: 382 HIPAGTQVLINAWAIGREAATWGPDVEEFRPERHLDSSVDYRGQAFELIPFGSGRRICPA 441

Query: 443 MQMGLIVVHLVLAQLVHCFDWKLPNNILPAELDMTEEFGLTCREL 483
           +   +++  +VLA LVH FDW+L       + ++ E  G+    +
Sbjct: 442 ISFAVVLNEVVLANLVHRFDWRLSVESTEDQTEVAESTGIAIHRM 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A068Q5V67.9e-17661.27Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1[more]
H2DH185.8e-13450.51Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
P244651.2e-11042.15Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
Q9LVD21.3e-10441.19Cytochrome P450 71B10 OS=Arabidopsis thaliana OX=3702 GN=CYP71B10 PE=3 SV=1[more]
Q50EK41.3e-10441.36Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022950432.13.3e-20372.26cytochrome P450 CYP736A12-like [Cucurbita moschata][more]
XP_023545010.12.4e-20172.26cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo][more]
XP_022978479.12.1e-19770.81cytochrome P450 CYP736A12-like [Cucurbita maxima][more]
XP_023544864.13.2e-19067.29cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo][more]
XP_022133318.11.6e-18968.04cytochrome P450 CYP736A12-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1GET01.6e-20372.26cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=... [more]
A0A6J1IU541.0e-19770.81cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478446 PE=3 ... [more]
A0A6J1BYU07.7e-19068.04cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE... [more]
A0A6J1GET52.1e-18766.67cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453534 PE=... [more]
A0A6J1BWD42.7e-18766.94cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005329 PE... [more]
Match NameE-valueIdentityDescription
AT5G57260.18.9e-10641.19cytochrome P450, family 71, subfamily B, polypeptide 10 [more]
AT3G26310.11.3e-10441.27cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
AT3G26300.16.0e-10240.66cytochrome P450, family 71, subfamily B, polypeptide 34 [more]
AT5G07990.13.0e-10141.91Cytochrome P450 superfamily protein [more]
AT3G48270.13.9e-10139.14cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 349..360
score: 43.62
coord: 426..435
score: 58.92
coord: 296..313
score: 38.48
IPR001128Cytochrome P450PFAMPF00067p450coord: 33..469
e-value: 4.0E-101
score: 339.1
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 305..331
score: 40.85
coord: 425..435
score: 55.18
coord: 60..79
score: 30.95
coord: 84..105
score: 25.75
coord: 177..195
score: 26.56
coord: 285..302
score: 33.54
coord: 389..413
score: 34.74
coord: 435..458
score: 22.43
coord: 348..366
score: 35.43
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 21..486
e-value: 8.4E-127
score: 425.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 33..482
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 1..464
e-value: 3.9E-18
score: 63.4
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 1..483
NoneNo IPR availablePANTHERPTHR47943:SF2CYTOCHROME P450 FAMILY 71 PROTEINcoord: 1..483
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 428..437

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0016631.1Tan0016631.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen