Tan0016585 (gene) Snake gourd v1

Overview


Name	Tan0016585
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	metalloendoproteinase 1-like
Location	LG11: 5151164 .. 5152063 (-)
RNA-Seq Expression	Tan0016585
Synteny	Tan0016585

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTAAAATTCTCTCTCCAATTCTTCCTACTTCTCGCCTCCATAGCACCTCATGTTTTCTCGGCACGCGATCTCCACATCCACAAATCATCTCGATTTCTATTTCCTCGACATCTCGTAGGAAGTCGCAGGGGTCACAACACAGAAGGAATCCACATCGTCAAAAATTACCTCCAACGTTACGGTTACTTAAGCAACAATATTAATGTCGAGACCAATGCAAACGCGTTCGACAACAACTTGGAAATGGCCATTAAAACATACCAAAAATTCTTCAAGCTGAACGTGAGCGGCATTTTGGACGAGGAGACACTACACGTAATGTCGCAGCCTCGATGTGCGGTTCCCGACGTGTTCAACAACAAAACGAGGCTGACAGGATCGGGCGTAGGAACTGATTTTGAATTTTTCAATGAAGGGAAACCAAAATGGCCGGAGTCGAAATACCATCTGAAATACGCATTTATTAACAATTTCCTATACTTCTACAAGAAGCCAGTGGTTGAAGCGTTGAAGACATGGGCTTCGTACACAGCATTTAAATTCTCAGAAGCTATTGAGGCAGAAGCAGATGTTAGAATAAGCTTTGAAATAGGGGACCATGGAGATGGATATCCTTTCTGGTATAGAGAGGGAGTTTTGGCGCATGCTTTTGGGCCTGGAGATGGGAGGCTTCACTTCAATATGCAGATGAGTTGGTGGGATGGAGCTTTTCCTGATAGCTATGATGTGTACTCAGTGGCATTGCATGAGATGGGACATGTTCTTGGGCTCGGCCACAGCACTGTAAAATACGCCATTATGTATCCTTCTATACCTCCCAATACTGTCAAGGACGTACAAAACGATGATGTTGATGGACTTTGGGAGTTGTATGAAGGATTTCAAGGACATTAA

mRNA sequence

ATGGATTTAAAATTCTCTCTCCAATTCTTCCTACTTCTCGCCTCCATAGCACCTCATGTTTTCTCGGCACGCGATCTCCACATCCACAAATCATCTCGATTTCTATTTCCTCGACATCTCGTAGGAAGTCGCAGGGGTCACAACACAGAAGGAATCCACATCGTCAAAAATTACCTCCAACGTTACGGTTACTTAAGCAACAATATTAATGTCGAGACCAATGCAAACGCGTTCGACAACAACTTGGAAATGGCCATTAAAACATACCAAAAATTCTTCAAGCTGAACGTGAGCGGCATTTTGGACGAGGAGACACTACACGTAATGTCGCAGCCTCGATGTGCGGTTCCCGACGTGTTCAACAACAAAACGAGGCTGACAGGATCGGGCGTAGGAACTGATTTTGAATTTTTCAATGAAGGGAAACCAAAATGGCCGGAGTCGAAATACCATCTGAAATACGCATTTATTAACAATTTCCTATACTTCTACAAGAAGCCAGTGGTTGAAGCGTTGAAGACATGGGCTTCGTACACAGCATTTAAATTCTCAGAAGCTATTGAGGCAGAAGCAGATGTTAGAATAAGCTTTGAAATAGGGGACCATGGAGATGGATATCCTTTCTGGTATAGAGAGGGAGTTTTGGCGCATGCTTTTGGGCCTGGAGATGGGAGGCTTCACTTCAATATGCAGATGAGTTGGTGGGATGGAGCTTTTCCTGATAGCTATGATGTGTACTCAGTGGCATTGCATGAGATGGGACATGTTCTTGGGCTCGGCCACAGCACTGTAAAATACGCCATTATGTATCCTTCTATACCTCCCAATACTGTCAAGGACGTACAAAACGATGATGTTGATGGACTTTGGGAGTTGTATGAAGGATTTCAAGGACATTAA

Coding sequence (CDS)

ATGGATTTAAAATTCTCTCTCCAATTCTTCCTACTTCTCGCCTCCATAGCACCTCATGTTTTCTCGGCACGCGATCTCCACATCCACAAATCATCTCGATTTCTATTTCCTCGACATCTCGTAGGAAGTCGCAGGGGTCACAACACAGAAGGAATCCACATCGTCAAAAATTACCTCCAACGTTACGGTTACTTAAGCAACAATATTAATGTCGAGACCAATGCAAACGCGTTCGACAACAACTTGGAAATGGCCATTAAAACATACCAAAAATTCTTCAAGCTGAACGTGAGCGGCATTTTGGACGAGGAGACACTACACGTAATGTCGCAGCCTCGATGTGCGGTTCCCGACGTGTTCAACAACAAAACGAGGCTGACAGGATCGGGCGTAGGAACTGATTTTGAATTTTTCAATGAAGGGAAACCAAAATGGCCGGAGTCGAAATACCATCTGAAATACGCATTTATTAACAATTTCCTATACTTCTACAAGAAGCCAGTGGTTGAAGCGTTGAAGACATGGGCTTCGTACACAGCATTTAAATTCTCAGAAGCTATTGAGGCAGAAGCAGATGTTAGAATAAGCTTTGAAATAGGGGACCATGGAGATGGATATCCTTTCTGGTATAGAGAGGGAGTTTTGGCGCATGCTTTTGGGCCTGGAGATGGGAGGCTTCACTTCAATATGCAGATGAGTTGGTGGGATGGAGCTTTTCCTGATAGCTATGATGTGTACTCAGTGGCATTGCATGAGATGGGACATGTTCTTGGGCTCGGCCACAGCACTGTAAAATACGCCATTATGTATCCTTCTATACCTCCCAATACTGTCAAGGACGTACAAAACGATGATGTTGATGGACTTTGGGAGTTGTATGAAGGATTTCAAGGACATTAA

Protein sequence

MDLKFSLQFFLLLASIAPHVFSARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYLQRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDVFNNKTRLTGSGVGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEALKTWASYTAFKFSEAIEAEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSWWDGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLWELYEGFQGH

Homology

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BLAST of Tan0016585 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 181.4 bits (459), Expect = 1.5e-44
Identity = 104/258 (40.31%), Postives = 140/258 (54.26%), Query Frame = 0


Query: 46  GHNTEGIHIVKNYLQRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEET 105
           G N +G+  VKNY    GY+ N  + + N   FD+ L  AIKTYQK + LNV+G  D  T
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHFDDN---FDDTLVSAIKTYQKNYNLNVTGKFDINT 107

Query: 106 LHVMSQPRCAVPDVFNNKTRLTGSGVGTDFEFFNEGKPKWPESKYHLKYAFI--NNFLYF 165
           L  +  PRC VPD+  N  + T  G+ +D+ FF +  P+W      L YAF         
Sbjct: 108 LKQIMTPRCGVPDIIINTNKTTSFGMISDYTFFKD-MPRWQAGTTQLTYAFSPEPRLDDT 167

Query: 166 YKKPVVEALKTWASYTAFKFSEAIEAE-ADVRISFEIGDHGDGYPFWYREGVLAHAFGPG 225
           +K  +  A   W       F E    E A+++I F   +HGD YPF    G+L HAF P 
Sbjct: 168 FKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPT 227

Query: 226 DGRLHFNMQMSW------WDGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPN 285
           DGR HF+    W             ++D+ SVA+HE+GH+LGLGHS+   AIMYPSIPP 
Sbjct: 228 DGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPR 287

Query: 286 TVK-DVQNDDVDGLWELY 294
           T K ++  DD+DG+ +LY
Sbjct: 288 TRKVNLAQDDIDGIRKLY 301

[Close]

BLAST of Tan0016585 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.7e-40
Identity = 114/306 (37.25%), Postives = 161/306 (52.61%), Query Frame = 0


Query: 6   SLQFFLLLASIAPHVFSARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYLQRYGYL 65
           S +F+  ++SI P  F    L+  +++   F + L G   G N  G+  +K Y +R+GY+
Sbjct: 19  SAKFYTNVSSIPPLQF----LNATQNAWETFSK-LAGCHIGENINGLSKLKQYFRRFGYI 78

Query: 66  SNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDVFNNKTR 125
           +   N     + FD+ L+ AI TYQK F L V+G LD  TL  + +PRC  PD+ +  + 
Sbjct: 79  TTTGNC---TDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSE 138

Query: 126 LTGSGV--GTDFEFFNEGKPKWPESKYHLKYAFI--NNFLYFYKKPVVEALKTWASYTAF 185
           + G  +   T+   F  GKP+WP+ K  L YAF   NN     K+    A   WA  T  
Sbjct: 139 MNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPL 198

Query: 186 KF--SEAIEAEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSWWDGAF 245
            F  SE+I   AD+ I F  G+HGDG PF    G LAHA  P  G LH +    W     
Sbjct: 199 NFTRSESI-LRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNG 258

Query: 246 PDS---------YDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVK-DVQNDDVDGL 296
             S          D+ SVA+HE+GH+LGLGHS+V+ AIM+P+I     K ++  DD++G+
Sbjct: 259 EISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGI 315

[Close]

BLAST of Tan0016585 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.5e-40
Identity = 104/286 (36.36%), Postives = 150/286 (52.45%), Query Frame = 0


Query: 39  HLVGSRRGHNTEGIHIVKNYLQRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVS 98
           +  G   G N +G++ +K Y QR+GY+    +     + FD+ L+ A++ YQ  F LNV+
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFS-GNFTDDFDDILKAAVELYQTNFNLNVT 105

Query: 99  GILDEETLHVMSQPRCAVPDVFNNKTRLTGSGVGTDFEFFNE-------------GKPKW 158
           G LD  T+  +  PRC  PDV N  + + G    T    F+              G+P+W
Sbjct: 106 GELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRW 165

Query: 159 PESKYHLKYAFINNFLYFYKKPVVEALKT--------WASYTAFKF--SEAIEAEADVRI 218
           P ++  L YAF        K P+ E +K+        W+  TA  F  SE+  + +D+ I
Sbjct: 166 PRNRRDLTYAFDP------KNPLTEEVKSVFSRAFGRWSDVTALNFTLSESF-STSDITI 225

Query: 219 SFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSWWDGAFPDSY-------DVYSV 278
            F  GDHGDG PF    G LAHAF P  G+ H +   +W      DS+       D+ SV
Sbjct: 226 GFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESV 285

Query: 279 ALHEMGHVLGLGHSTVKYAIMYPSIPPNTVK-DVQNDDVDGLWELY 294
           A+HE+GH+LGLGHS+V+ +IMYP+I     K D+ NDDV+G+  LY
Sbjct: 286 AVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of Tan0016585 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.6e-38
Identity = 116/314 (36.94%), Postives = 160/314 (50.96%), Query Frame = 0


Query: 10  FLLLASIAPHVFSARD--------------LHIHKSSRFLFPRHLVGSRRGHNTEGIHIV 69
           F+L+    P+ F AR+              +H+  S+   F R LV  + G +  G+  +
Sbjct: 15  FVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSR-LVDVQIGSHVSGVSEL 74

Query: 70  KNYLQRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCA 129
           K YL R+GY+  N   E  ++ FD  LE AI  YQ+   L ++G LD  T+ +MS PRC 
Sbjct: 75  KRYLHRFGYV--NDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCG 134

Query: 130 VPD---VFNNKTRLTGSGVGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFY------KK 189
           V D     NN    T +     + +FN GKPKW  ++  L YA        Y      K 
Sbjct: 135 VSDTHMTINNDFLHTTA----HYTYFN-GKPKW--NRDTLTYAISKTHKLDYLTSEDVKT 194

Query: 190 PVVEALKTWASYTAFKFSEAIE-AEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGR 249
               A   W+S     F E  +   AD++I F  GDHGDG PF    G LAHAF P +GR
Sbjct: 195 VFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGR 254

Query: 250 LHFNMQMSW-----WDGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVK- 294
           LH +   +W       G+   + D+ SVA HE+GH+LGLGHS+ + A+MYPS+ P T K 
Sbjct: 255 LHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKV 314

[Close]

BLAST of Tan0016585 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 6.7e-37
Identity = 98/281 (34.88%), Postives = 147/281 (52.31%), Query Frame = 0


Query: 39  HLVGSRRGHNTEGIHIVKNYLQRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVS 98
           +  G   G   +G++++K Y Q +GY++         + FD+ L+ A++ YQ+ F+LNV+
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVT 107

Query: 99  GILDEETLHVMSQPRCAVPDVFNNKT---------RLTGSGVGTDFE-----FFNEGKPK 158
           G+LDE TL  +  PRC  PDV N  +          ++ +G G  F       F  G+P+
Sbjct: 108 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 167

Query: 159 WPESKYHLKYAF--INNFLYFYKKPVVEALKTWASYTAFKFSEAIE-AEADVRISFEIGD 218
           WP ++  L YAF   N      K     A   W   T   F+     + +D+ I F  G+
Sbjct: 168 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGE 227

Query: 219 HGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSW------WDG--AFPDSYDVYSVALHEM 278
           HGDG PF      LAHAF P  G  H + + +W       DG  +  ++ D+ SVA+HE+
Sbjct: 228 HGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEI 287

Query: 279 GHVLGLGHSTVKYAIMYPSIPPNTVK-DVQNDDVDGLWELY 294
           GH+LGLGHS+V+ +IMYP+I     K D+  DDV+G+  LY
Sbjct: 288 GHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of Tan0016585 vs. NCBI nr
Match: XP_023544665.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 378.6 bits (971), Expect = 4.9e-101
Identity = 187/301 (62.13%), Postives = 221/301 (73.42%), Query Frame = 0


Query: 1   MDLKFSLQ-FFLLLASIAPHVFSARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYL 60
           MDL   LQ  F+L+A+IAPH   AR LH HK S  +FP+HLVGS +GHN EGIH V+ +L
Sbjct: 1   MDLTSCLQVMFVLVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 61  QRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDV 120
           QRYGYLS   NVET  NAFD+NLE A+K+YQKFF LNVSGILD ETLH+MSQPRC VPDV
Sbjct: 61  QRYGYLST--NVETKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDV 120

Query: 121 FNNKTRLTGS---GVGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEALKTWA 180
           F N+T   G     +G D+ FF+E KPKW  SKY+LKYAF+ NF   YK  V EA  TW+
Sbjct: 121 FINETSGEGDRDHSIGLDYTFFHEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWS 180

Query: 181 SYTAFKFSEAIEAEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSWWD 240
           S T F F+E      DV+ISFE+GDHGDGYPF    GVLAHAF P DGRLH+N Q +W D
Sbjct: 181 SVTGFTFTEGSADNTDVKISFEVGDHGDGYPFEDGSGVLAHAFAPEDGRLHYNAQEAWAD 240

Query: 241 GAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLWELYEGF 298
           GA+PD YDV +VALHE+GHVLGLGHS  +YA+M+P  PPN V  + NDDV+G+  LY GF
Sbjct: 241 GAYPDKYDVGTVALHELGHVLGLGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALYNGF 299

[Close]

BLAST of Tan0016585 vs. NCBI nr
Match: KAG6602464.1 (hypothetical protein SDJN03_07697, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.9 bits (969), Expect = 8.3e-101
Identity = 187/303 (61.72%), Postives = 221/303 (72.94%), Query Frame = 0


Query: 1   MDLKFSLQ-FFLLLASIAPHVFSARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYL 60
           MDLK  LQ  F+L+A+IAPH   AR LH HK S  +FP+HLVGS +GHN EGIH V+ YL
Sbjct: 1   MDLKSCLQVMFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNMEGIHNVRAYL 60

Query: 61  QRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDV 120
           QRYGYLS   NV+T  NAFD+NLE A+K+YQKFF LNVSGILD ETL +MSQPRC VPDV
Sbjct: 61  QRYGYLST--NVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLELMSQPRCGVPDV 120

Query: 121 FNNKTRLTGSG-----VGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEALKT 180
           F+N+T   G G     +G D+ FF E KPKW  SKY+LKYAF+ NF   YK  V EA  T
Sbjct: 121 FSNETGGGGDGDWDHSIGIDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREATNT 180

Query: 181 WASYTAFKFSEAIEAEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSW 240
           W+S T F F+E      DV+ISFE+GDHGDGYPF    GVLAHAF P DGRLHFN + +W
Sbjct: 181 WSSVTGFTFTEGSADNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTRHAW 240

Query: 241 WDGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLWELYE 298
            DGA+P  YDV +VALHE+GHVLGLGHS  +YA+M+P  PPN V  + NDDV+G+  LY 
Sbjct: 241 ADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPYTPPNFVTGLDNDDVNGIMALYN 300

[Close]

BLAST of Tan0016585 vs. NCBI nr
Match: KAG6602463.1 (hypothetical protein SDJN03_07696, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 373.6 bits (958), Expect = 1.6e-99
Identity = 184/303 (60.73%), Postives = 219/303 (72.28%), Query Frame = 0


Query: 1   MDLKFSLQ-FFLLLASIAPHVFSARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYL 60
           MDL   LQ  F+L+  IAPH   AR LH HK S  +FP+HLVGS +GHN EGIH V+ +L
Sbjct: 1   MDLTSCLQLMFILVTVIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 61  QRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDV 120
           QRYGYL    NV+T  NAFD+NLE A+K+YQKFF LNVSGILD ETLH+MSQPRC VPDV
Sbjct: 61  QRYGYL--RTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDV 120

Query: 121 FNNKTRLTGSG-----VGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEALKT 180
           F N+T   G G     +G D+ FF+E KPKW  SKY+LKYAF+ NF   YK  V EA  T
Sbjct: 121 FINETSGDGDGDWDHSIGLDYTFFHEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNT 180

Query: 181 WASYTAFKFSEAIEAEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSW 240
           W+S T F F+E      DV+ISFE+GDHGDGYPF    GVLAHAF P DGRLH+N + +W
Sbjct: 181 WSSVTGFTFTEGSADNTDVKISFEVGDHGDGYPFEEGSGVLAHAFAPEDGRLHYNAREAW 240

Query: 241 WDGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLWELYE 298
            DGA+PD YDV +VALHE+GHVLGLGHS  +YA+M+P  PPN V  + NDDV+G+  LY 
Sbjct: 241 ADGAYPDKYDVGTVALHELGHVLGLGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALYN 300

[Close]

BLAST of Tan0016585 vs. NCBI nr
Match: XP_023544311.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.4 bits (947), Expect = 2.9e-98
Identity = 181/303 (59.74%), Postives = 225/303 (74.26%), Query Frame = 0


Query: 1   MDLKFSLQ-FFLLLASIAPHVFSARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYL 60
           MDLK  LQ  F+L+A+IAPH   AR LH HK S  +FP+HLVGS +GHN EGIH V+ +L
Sbjct: 1   MDLKSCLQVMFILVAAIAPHAIMARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 61  QRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDV 120
           QRYGYLS   NV+T  NAFD+NLE A+K+YQKFF LNVSGILD ETL +MSQPRC VPDV
Sbjct: 61  QRYGYLST--NVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLELMSQPRCGVPDV 120

Query: 121 FNNKTRLTGSG-----VGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEALKT 180
           F N+T   G+G     +G  + FFN+ KPKW  SKY+LKY F+ +F   YK  VV+A+ +
Sbjct: 121 FINETGGDGNGDRDHPIGLHYRFFNDSKPKWSASKYNLKYTFVRSFPEQYKSTVVQAMIS 180

Query: 181 WASYTAFKFSEAIEAEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSW 240
           W+  + F FSE    + DVRISFE+G+HGDG+PF    GVLAHAF P DGRLHFN   +W
Sbjct: 181 WSLNSVFAFSEGSTDDTDVRISFEVGEHGDGHPFKKGSGVLAHAFAPEDGRLHFNEWHAW 240

Query: 241 WDGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLWELYE 298
            DGA+PD YDV SVALHE+GH+LGLGHS ++ A+M+PS+ PNT K++ +DDV G+  LY+
Sbjct: 241 ADGAYPDKYDVGSVALHELGHILGLGHSDIRDAVMWPSMRPNTKKELSSDDVSGISALYD 300

[Close]

BLAST of Tan0016585 vs. NCBI nr
Match: XP_022959850.1 (metalloendoproteinase 1-like [Cucurbita moschata])

HSP 1 Score: 365.9 bits (938), Expect = 3.3e-97
Identity = 179/303 (59.08%), Postives = 223/303 (73.60%), Query Frame = 0


Query: 1   MDLKFSLQ-FFLLLASIAPHVFSARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYL 60
           MDL+  LQ  F+L+A+IAPH   AR LHIHK S  +FP+HLVGS +GHN EGIH V+ +L
Sbjct: 1   MDLRSCLQVMFILVAAIAPHAIMARHLHIHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 61  QRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDV 120
           QRYGYLS   NVET  N FD+NLE AIK+YQKFF LNVSGILD ETL +MSQPRC VPDV
Sbjct: 61  QRYGYLST--NVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDV 120

Query: 121 FNNKTRLTGSG-----VGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEALKT 180
           F N+T   G+G     +G  + FFN+ KPKW  SKY+LKY F+ +F   YK  V++A+ +
Sbjct: 121 FINETGGDGNGDRDHPIGLHYSFFNDLKPKWSASKYNLKYTFLGSFPEQYKNTVIQAMIS 180

Query: 181 WASYTAFKFSEAIEAEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSW 240
           W+  + F FSE      DVRISFE+GDHGDG PF  + G LAHAF P DGRLHFN Q +W
Sbjct: 181 WSFNSVFAFSEGSTDNTDVRISFEVGDHGDGSPFKDKNGALAHAFAPEDGRLHFNAQHAW 240

Query: 241 WDGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLWELYE 298
            DGA+PD +DV SVALHE+GH+LGLGHS ++ A+M+PS+PPN  K++ +DDV G+  LY+
Sbjct: 241 ADGAYPDKFDVGSVALHELGHILGLGHSDIRDAVMWPSMPPNLKKELSSDDVSGISALYD 300

[Close]

BLAST of Tan0016585 vs. ExPASy TrEMBL
Match: A0A6J1H7I1 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.6e-97
Identity = 179/303 (59.08%), Postives = 223/303 (73.60%), Query Frame = 0


Query: 1   MDLKFSLQ-FFLLLASIAPHVFSARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYL 60
           MDL+  LQ  F+L+A+IAPH   AR LHIHK S  +FP+HLVGS +GHN EGIH V+ +L
Sbjct: 1   MDLRSCLQVMFILVAAIAPHAIMARHLHIHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFL 60

Query: 61  QRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDV 120
           QRYGYLS   NVET  N FD+NLE AIK+YQKFF LNVSGILD ETL +MSQPRC VPDV
Sbjct: 61  QRYGYLST--NVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDV 120

Query: 121 FNNKTRLTGSG-----VGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEALKT 180
           F N+T   G+G     +G  + FFN+ KPKW  SKY+LKY F+ +F   YK  V++A+ +
Sbjct: 121 FINETGGDGNGDRDHPIGLHYSFFNDLKPKWSASKYNLKYTFLGSFPEQYKNTVIQAMIS 180

Query: 181 WASYTAFKFSEAIEAEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSW 240
           W+  + F FSE      DVRISFE+GDHGDG PF  + G LAHAF P DGRLHFN Q +W
Sbjct: 181 WSFNSVFAFSEGSTDNTDVRISFEVGDHGDGSPFKDKNGALAHAFAPEDGRLHFNAQHAW 240

Query: 241 WDGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLWELYE 298
            DGA+PD +DV SVALHE+GH+LGLGHS ++ A+M+PS+PPN  K++ +DDV G+  LY+
Sbjct: 241 ADGAYPDKFDVGSVALHELGHILGLGHSDIRDAVMWPSMPPNLKKELSSDDVSGISALYD 300

[Close]

BLAST of Tan0016585 vs. ExPASy TrEMBL
Match: A0A6J1H7Q4 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460843 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.7e-96
Identity = 181/297 (60.94%), Postives = 212/297 (71.38%), Query Frame = 0


Query: 1   MDLKFSLQ-FFLLLASIAPHVFSARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYL 60
           MDLK  LQ  F+L+A+IAPH   AR LH HK S  +FP+HLVGS +GHN EGIH V+ +L
Sbjct: 1   MDLKSCLQLMFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNMEGIHNVRAFL 60

Query: 61  QRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDV 120
           QRYGYLS   NV+T  NAFD+NLE A+K+YQKFF LNVSGILD ETL +MSQPRC VPDV
Sbjct: 61  QRYGYLST--NVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGVPDV 120

Query: 121 FNNKTRLTGSGVGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEALKTWASYT 180
           F             D+ FF E KPKW  SKY+LKYAF+ NF   YK  V EA  TW+S T
Sbjct: 121 FR-----------IDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSVT 180

Query: 181 AFKFSEAIEAEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSWWDGAF 240
            F F+E      DV+ISFE+GDHGDGYPF    GVLAHAF P DGRLHFN + +W DGA+
Sbjct: 181 GFTFTEGSVDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTRHAWADGAY 240

Query: 241 PDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLWELYEGF 297
           P  YDV +VALHE+GHVLGLGHS  +YA+M+P  PPN V  + NDDV+G+  LY GF
Sbjct: 241 PYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVTGLDNDDVNGIMALYNGF 284

[Close]

BLAST of Tan0016585 vs. ExPASy TrEMBL
Match: A0A6J1H5Y2 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460835 PE=3 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 2.5e-95
Identity = 174/280 (62.14%), Postives = 206/280 (73.57%), Query Frame = 0


Query: 23  ARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYLQRYGYLSNNINVETNANAFDNNL 82
           AR LH HK S  +FP+HLVGS +GHN EGIH V+ +LQRYGYLS   NVET  NAFD+NL
Sbjct: 2   ARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLST--NVETKGNAFDDNL 61

Query: 83  EMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDVFNNKT-----RLTGSGVGTDFEF 142
           E A+K+YQKFF LNVSGILD ETLH+MSQPRC VPDVF N+T     R     +G D+ F
Sbjct: 62  EFALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDVFINETSGDRDRDWDHSIGLDYTF 121

Query: 143 FNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEALKTWASYTAFKFSEAIEAEADVRISF 202
           F+E KPKW  SKY+LKYAF+ NF   YK  V EA  TW+S T F F+E      DV+ISF
Sbjct: 122 FHEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSVTGFTFTEGSADNTDVKISF 181

Query: 203 EIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSWWDGAFPDSYDVYSVALHEMGHVL 262
           E+GDHGDGYPF    GVLAHAF P DGRLH+N + +W DGA+PD YDV +VALHE+GHVL
Sbjct: 182 EVGDHGDGYPFEEGSGVLAHAFAPEDGRLHYNAREAWADGAYPDKYDVGTVALHELGHVL 241

Query: 263 GLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLWELYEGFQ 298
           GLGHS  +YA+M+P  PPN V  + NDDV+G+  LY GF+
Sbjct: 242 GLGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALYNGFE 279

[Close]

BLAST of Tan0016585 vs. ExPASy TrEMBL
Match: A0A6J1H620 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460796 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 2.1e-89
Identity = 166/280 (59.29%), Postives = 206/280 (73.57%), Query Frame = 0


Query: 23  ARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYLQRYGYLSNNINVETNANAFDNNL 82
           AR LH HK S  +FP+HLVGS +GHN EGIH V+ +LQRYGYLS   NVET  N FD+NL
Sbjct: 2   ARHLHTHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLST--NVETKGNVFDDNL 61

Query: 83  EMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDVFNNKTRLTGSG-----VGTDFEF 142
           E AIK+YQKFF LNVSGILD ETL +MSQPRC VPDVF N+T    +G     +G  + F
Sbjct: 62  EFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETSGDRNGDGDHPIGLHYAF 121

Query: 143 FNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEALKTWASYTAFKFSEAIEAEADVRISF 202
            N+ KPKW  SKY+LKYAF+ +F   YK  VV A+ +W+S + FKF++    +ADV+ISF
Sbjct: 122 INDLKPKWGASKYNLKYAFVGSFPKQYKATVVGAMHSWSSKSGFKFTKGSANKADVKISF 181

Query: 203 EIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSWWDGAFPDSYDVYSVALHEMGHVL 262
           E+GDHGDG+PF    G L HAF P DGRLHFN + +W DGA+PD +DV SVALHE+GH+L
Sbjct: 182 EVGDHGDGHPFKEGSGELGHAFAPEDGRLHFNAREAWADGAYPDKFDVGSVALHELGHIL 241

Query: 263 GLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLWELYEGFQ 298
           GLGHS+ + A+M+PSI PN V  + +DDV+GL  LY GF+
Sbjct: 242 GLGHSSFRNAVMWPSIGPNFVMGLNDDDVNGLLALYNGFR 279

[Close]

BLAST of Tan0016585 vs. ExPASy TrEMBL
Match: A0A6J1BVR3 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 1.5e-79
Identity = 164/303 (54.13%), Postives = 212/303 (69.97%), Query Frame = 0


Query: 3   LKFSLQFFLLLASIAPHVFSARDLHIH---KSSRFLFPRHLVGSRRGHNTEGIHIVKNYL 62
           L  SL  F+LLA+IA HV SAR LH H   KSS  +F ++L+GSR+GHN +GIH VK YL
Sbjct: 8   LHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYL 67

Query: 63  QRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDV 122
           +RYGY ++     T +NAFD++LE A+KTYQKFF LNVSGILD ETL +MSQPRCAVPD+
Sbjct: 68  KRYGYFTH-----TQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVPDI 127

Query: 123 FNNKTRL-TGSG------VGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFYKKPVVEAL 182
           F+N+T    G G      +G+ + FF    PKWPESK  L+Y++ ++F   +K PV  AL
Sbjct: 128 FHNETSTRKGWGHEEHLHMGSHYLFFPR-TPKWPESKCRLRYSYYHDFPMAFKDPVNNAL 187

Query: 183 KTWASYTAFKFSEAIEAE--ADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNM 242
           + W++Y  F F E  E +  AD++ISFE+G+HGDG+PF    GVLAHAF P DGR HFN+
Sbjct: 188 ELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNV 247

Query: 243 QMSWWDGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVKDVQNDDVDGLW 294
           +  W  GA P+  DV SVA+HE+GH+LGLGHS  + AIM+P I PN VK +  D+V G+ 
Sbjct: 248 EQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGIK 304

[Close]

BLAST of Tan0016585 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 167.9 bits (424), Expect = 1.2e-41
Identity = 114/306 (37.25%), Postives = 161/306 (52.61%), Query Frame = 0


Query: 6   SLQFFLLLASIAPHVFSARDLHIHKSSRFLFPRHLVGSRRGHNTEGIHIVKNYLQRYGYL 65
           S +F+  ++SI P  F    L+  +++   F + L G   G N  G+  +K Y +R+GY+
Sbjct: 19  SAKFYTNVSSIPPLQF----LNATQNAWETFSK-LAGCHIGENINGLSKLKQYFRRFGYI 78

Query: 66  SNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCAVPDVFNNKTR 125
           +   N     + FD+ L+ AI TYQK F L V+G LD  TL  + +PRC  PD+ +  + 
Sbjct: 79  TTTGNC---TDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSE 138

Query: 126 LTGSGV--GTDFEFFNEGKPKWPESKYHLKYAFI--NNFLYFYKKPVVEALKTWASYTAF 185
           + G  +   T+   F  GKP+WP+ K  L YAF   NN     K+    A   WA  T  
Sbjct: 139 MNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPL 198

Query: 186 KF--SEAIEAEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSWWDGAF 245
            F  SE+I   AD+ I F  G+HGDG PF    G LAHA  P  G LH +    W     
Sbjct: 199 NFTRSESI-LRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNG 258

Query: 246 PDS---------YDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVK-DVQNDDVDGL 296
             S          D+ SVA+HE+GH+LGLGHS+V+ AIM+P+I     K ++  DD++G+
Sbjct: 259 EISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGI 315

[Close]

BLAST of Tan0016585 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 165.6 bits (418), Expect = 6.0e-41
Identity = 104/286 (36.36%), Postives = 150/286 (52.45%), Query Frame = 0


Query: 39  HLVGSRRGHNTEGIHIVKNYLQRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVS 98
           +  G   G N +G++ +K Y QR+GY+    +     + FD+ L+ A++ YQ  F LNV+
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFS-GNFTDDFDDILKAAVELYQTNFNLNVT 105

Query: 99  GILDEETLHVMSQPRCAVPDVFNNKTRLTGSGVGTDFEFFNE-------------GKPKW 158
           G LD  T+  +  PRC  PDV N  + + G    T    F+              G+P+W
Sbjct: 106 GELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRW 165

Query: 159 PESKYHLKYAFINNFLYFYKKPVVEALKT--------WASYTAFKF--SEAIEAEADVRI 218
           P ++  L YAF        K P+ E +K+        W+  TA  F  SE+  + +D+ I
Sbjct: 166 PRNRRDLTYAFDP------KNPLTEEVKSVFSRAFGRWSDVTALNFTLSESF-STSDITI 225

Query: 219 SFEIGDHGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSWWDGAFPDSY-------DVYSV 278
            F  GDHGDG PF    G LAHAF P  G+ H +   +W      DS+       D+ SV
Sbjct: 226 GFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESV 285

Query: 279 ALHEMGHVLGLGHSTVKYAIMYPSIPPNTVK-DVQNDDVDGLWELY 294
           A+HE+GH+LGLGHS+V+ +IMYP+I     K D+ NDDV+G+  LY
Sbjct: 286 AVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of Tan0016585 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 160.2 bits (404), Expect = 2.5e-39
Identity = 116/314 (36.94%), Postives = 160/314 (50.96%), Query Frame = 0


Query: 10  FLLLASIAPHVFSARD--------------LHIHKSSRFLFPRHLVGSRRGHNTEGIHIV 69
           F+L+    P+ F AR+              +H+  S+   F R LV  + G +  G+  +
Sbjct: 15  FVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSR-LVDVQIGSHVSGVSEL 74

Query: 70  KNYLQRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRCA 129
           K YL R+GY+  N   E  ++ FD  LE AI  YQ+   L ++G LD  T+ +MS PRC 
Sbjct: 75  KRYLHRFGYV--NDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCG 134

Query: 130 VPD---VFNNKTRLTGSGVGTDFEFFNEGKPKWPESKYHLKYAFINNFLYFY------KK 189
           V D     NN    T +     + +FN GKPKW  ++  L YA        Y      K 
Sbjct: 135 VSDTHMTINNDFLHTTA----HYTYFN-GKPKW--NRDTLTYAISKTHKLDYLTSEDVKT 194

Query: 190 PVVEALKTWASYTAFKFSEAIE-AEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGR 249
               A   W+S     F E  +   AD++I F  GDHGDG PF    G LAHAF P +GR
Sbjct: 195 VFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGR 254

Query: 250 LHFNMQMSW-----WDGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVK- 294
           LH +   +W       G+   + D+ SVA HE+GH+LGLGHS+ + A+MYPS+ P T K 
Sbjct: 255 LHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKV 314

[Close]

BLAST of Tan0016585 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 156.0 bits (393), Expect = 4.8e-38
Identity = 98/281 (34.88%), Postives = 147/281 (52.31%), Query Frame = 0


Query: 39  HLVGSRRGHNTEGIHIVKNYLQRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVS 98
           +  G   G   +G++++K Y Q +GY++         + FD+ L+ A++ YQ+ F+LNV+
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVT 107

Query: 99  GILDEETLHVMSQPRCAVPDVFNNKT---------RLTGSGVGTDFE-----FFNEGKPK 158
           G+LDE TL  +  PRC  PDV N  +          ++ +G G  F       F  G+P+
Sbjct: 108 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 167

Query: 159 WPESKYHLKYAF--INNFLYFYKKPVVEALKTWASYTAFKFSEAIE-AEADVRISFEIGD 218
           WP ++  L YAF   N      K     A   W   T   F+     + +D+ I F  G+
Sbjct: 168 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGE 227

Query: 219 HGDGYPFWYREGVLAHAFGPGDGRLHFNMQMSW------WDG--AFPDSYDVYSVALHEM 278
           HGDG PF      LAHAF P  G  H + + +W       DG  +  ++ D+ SVA+HE+
Sbjct: 228 HGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEI 287

Query: 279 GHVLGLGHSTVKYAIMYPSIPPNTVK-DVQNDDVDGLWELY 294
           GH+LGLGHS+V+ +IMYP+I     K D+  DDV+G+  LY
Sbjct: 288 GHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of Tan0016585 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 142.1 bits (357), Expect = 7.1e-34
Identity = 97/252 (38.49%), Postives = 130/252 (51.59%), Query Frame = 0


Query: 55  VKNYLQRYGYLSNNINVETNANAFDNNLEMAIKTYQKFFKLNVSGILDEETLHVMSQPRC 114
           +K +LQ+YGYL        N  + D + E A+  YQK   L ++G  D +TL  +  PRC
Sbjct: 53  IKRHLQQYGYLPQ------NKESDDVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRC 112

Query: 115 AVPDVFNNKTRLTGSGVGTDFEFFNEGKPKWP-ESKYHLKYAFINNFLYFYKKPV----- 174
             PD    KT       G  + +F  G+P+W  +    L YAF    L  Y  P      
Sbjct: 113 GFPDDVEPKT--APFHTGKKYVYF-PGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRV 172

Query: 175 -VEALKTWASYTAFKFSEAIE-AEADVRISFEIGDHGDGYPFWYREGVLAHAFGPGDGRL 234
              A   WAS     F E  +   AD++I F  GDHGDG PF    GVLAH F P +GRL
Sbjct: 173 FRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRL 232

Query: 235 HFNMQMSWW----DGAFPDSYDVYSVALHEMGHVLGLGHSTVKYAIMYPSIPPNTVK-DV 294
           H +   +W     +     + D+ SVA+HE+GHVLGLGHS+VK A MYP++ P + K ++
Sbjct: 233 HLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNL 292

The following BLAST results are available for this feature:

BLAST of Tan0016585 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P29136	1.5e-44	40.31	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q9ZUJ5	1.7e-40	37.25	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O04529	8.5e-40	36.36	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
O23507	3.6e-38	36.94	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]
Q5XF51	6.7e-37	34.88	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]

BLAST of Tan0016585 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023544665.1	4.9e-101	62.13	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6602464.1	8.3e-101	61.72	hypothetical protein SDJN03_07697, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6602463.1	1.6e-99	60.73	hypothetical protein SDJN03_07696, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023544311.1	2.9e-98	59.74	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022959850.1	3.3e-97	59.08	metalloendoproteinase 1-like [Cucurbita moschata]	[more]

BLAST of Tan0016585 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H7I1	1.6e-97	59.08	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 ...	[more]
A0A6J1H7Q4	1.7e-96	60.94	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460843 PE=3 ...	[more]
A0A6J1H5Y2	2.5e-95	62.14	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460835 PE=3 ...	[more]
A0A6J1H620	2.1e-89	59.29	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460796 PE=3 ...	[more]
A0A6J1BVR3	1.5e-79	54.13	metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3...	[more]

BLAST of Tan0016585 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G59970.1	1.2e-41	37.25	Matrixin family protein	[more]
AT1G70170.1	6.0e-41	36.36	matrix metalloproteinase	[more]
AT4G16640.1	2.5e-39	36.94	Matrixin family protein	[more]
AT1G24140.1	4.8e-38	34.88	Matrixin family protein	[more]
AT2G45040.1	7.1e-34	38.49	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 281..294 score: 38.07 coord: 109..122 score: 53.57 coord: 191..219 score: 53.07 coord: 168..183 score: 29.73 coord: 248..273 score: 57.6
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 141..295 e-value: 1.5E-27 score: 107.6
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 43..296 e-value: 6.3E-68 score: 230.9
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 50..109 e-value: 1.5E-13 score: 50.7
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 165..293 e-value: 3.6E-34 score: 117.9
None	No IPR available	PANTHER	PTHR10201:SF272	METALLOENDOPROTEINASE 2-MMP-LIKE	coord: 24..293
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 24..293
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 140..293
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 45..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0016585.1	Tan0016585.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity