Tan0016494 (gene) Snake gourd v1

Overview


Name	Tan0016494
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Pectinesterase
Location	LG01: 1054518 .. 1057902 (-)
RNA-Seq Expression	Tan0016494
Synteny	Tan0016494

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGATGTTTGACATATTTAATTTCATATCTCAAAATTTTAATTAAAGAAAAAAAAAAAAAGAGTGACCAATAAGTTAATAACTTAGATAGTAAGACTTAAAAAAGATGGTCTCTCTCTCTAAAGATAACACAGTTCCAATCTCTCTCCATCTCCAATTGTAAGATAAAACACAGTGGGGAGTGGCCATAGTTTCAAGTAGCAGCCAGCTAATTCTCCCTTCGACGGCGACTTCTCACACCCACCACTCTCTCTCTAACTTCATATATATTTATATATTTTACTTAAAATCACTCAAAATCCCACCTCCACCTTTACCATTTCTCTCTCTCTCTCTTGTTTCTCTCTCCTCTGCAAAATATGGGTTATGGCCGCCTCAACGAAACCGACCCTGGTGGCTCCTCCGGCGCTGTAACTTTCCAGCTCTCCGACGACGCCGTTTCATTCCCCACTCCCTCTCCCAACCGAAGAAGGAGAACACGACTTCTCCTTCTCTCCCTTCTCTCCCTCATTCTCATCATCGCCGCCGCTGTCTCCGCCACTTTCCTCGTCGGACTCCGAACCGCCTCTTCCTCTCCCCGATCCGACCCGAATCTCCCTCGAAAACCCACTCAGGCCATTTCCAAGGCCTGTAGCCGAACTCGCTTCCCGACTCTCTGTGTCAACTCGCTACTCGATTTCCCTGGATCTCTCAACGCCAACGAACACGATCTCGTCCACATTTCCTTCAATTTAACTCTCCAGCATCTCAACAAAGCCCTCTATCTCTCCTCTGAGATTTCCTCTCTTCAAATGGATCTTCGCGTTCGCTCTGCCTATGACGCTTGTCTTGAACTCCTCGACGACTCTGTCGACGCTCTCACACGCTCCTTACAGTCCGTCGCGCCACCTTCCTCTGACGGCACCCCCCATCGCCTCGCCTCCTCCGATGACATCATCACCTGGCTCAGCGCCGCCCTCACCAACCAAGACACCTGTTCCGACGGATTCTCCGAACTTACTGGAAATGTTAAAGATCAAATGACGGATAAACTCCACAATTTATCCGAGCTTGTGAGCAATTGCTTGGCGCTGTTTTCCGGTAGTGAAACGAACGATTTCGCCGGAGTGCCGATTCAGAACAAGCGACGGTTGATGGACGCCGGGGAGAACGTGGTTAGTTCCGGTAACTTCCCCGGCTGGATGAGCCGGAGAGAGAGGCGGTTACTGACGCTGCCGGTGGGGGCGCTACAGGCCGATATTGTGGTTTCTAAGGACGGGAACGGAACGGTGAAGACGATTGCCGAAGCGATCAAAAAGGCTCCGGAATACAGTAGCAAAAGGACGATCATCTATGTGATGGCAGGAAGGTAAATTTAAGGTTAAATTACAAATTTAATCTCTAAACAAATAATGTATAATAATTTTTTGAACTTTTAATTTTGTGGCTAAAATCAATTACAAATTTAATCTCTAAACAACTGTATAATAATTTTTTAAATATTTAATTTTGTGATTAACCACATATGTAAAAAAGGGAAAAAAAATTGTTAAAATCAATCACATTTGATTTAAATTTAATTTGGTTTGATTTCTTTTCAACTACAACGCGTTTCGTTTGACTTGCGTGTCACGATCGTGAACTGTTTTCACGCGATTGAAAAAAGCAGGCGCCATACGAATTTGGCATGGTGAATTTACGGAAATGCCCTTTCTTCGTCCTTTTCTGCAGGTACGAAGAGAAGAATCTGAAGGTGGGAAGGAAGAAAACGAACCTGATGTTTGTTGGGGATGGAAAGGGTAAAACAGTCATTTCGGGTGCTAAGAGTATCTTTGACAACGTGACAACTTTTCACACGGCGTCGTTTGGTAATAAATTTATTCATCTAACTTTTCATTATTTATTACTTTTACGCTCTCTTTTCTTTTTATTTTAGTTCTTCACCAGAATAATTTAGGGGTGAGAATAATTCCTGGTTAAAAATGACTTTTGCAAAAAAAAAAAACCAACAACAGCCCACGTGTTAATGCAGCTTTGATTGCACTCGCACGTGCCGCCTCATAATCTCTTTGTTCTACTTGTCGAATTTTCTCAATTAGACCAAATTACCCTTCTATTATTGTCTCAATTACCGTTACAAAAAAACTCTCTATGGGCAAGAAGTGGAGGTCAGTGTCATATGCCACAATTTCATATCATATTTATTTTGTACCATTGCTCTAATTAAATTATCTAATTAATCCTTTTTTTAGTTCAATTATCCAATTAATCATATATCAATAGTATAATAATTCCCATCATTTGGACATACATTATTTAACTTAATCCCTCTTTTTGTCCATTGATCCATTGCATGCGAATGTCATTAATTTATTCTTTTAAACATTTATTTATATATTATTTGACCAATCTGAGACATTTTTTTCTTTTTTGGTCACAGCGGCCACCGGTGCCGGTATAATATTGAGGGACATGACATTTGAGAATTGGGCCGGACCGGGTCGACACCAGGCGGTGGCGCTCCGAGTCGGCGCCGACCACGCCGTCGTGTACCGCTGCAACGTAATCGGCTATCAAGACACTCTGTACGTCCATTCCAACCGCCAATTCTTTAGAGAGTGCGACATTTACGGGACCGTCGATTTCATCTTTGGCAATGCGGCGGTGGTGTTTCAGAACTGCAGCATTTACGCCCGAAAAGCGATGGACCTTCAAAAGAACACAATCACAGCCCAGAATCGGAAGGATCCGAATCAGAATACCGGAATTTCCATCCACGCCTGCCGGATTTTAGCCACCTCCGATCTGTCGTCGTCCAATAGCAGCAACCCGACGTATCTGGGCCGGCCGTGGAAATTATACTCTAGAGTCGTGTACATGCTATCGTACATAGGGAGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAATGCCACGTTTGCACTTGACACGTTGTATTACGGTGAGTATATGAATTATGGGCCGGGTGGGGCAGTGGGCCAGAGGGTGACGTGGCCTGGGTATCGGGTCATAACGTCCCCGGTGGAGGCGAGTAAGTTTACGGTGGCGCAGTTCATTTACGGCTCGTCGTGGTTGCCGTCCACTGGAGTTGCATTTCTTGGAGGGTTAAATGTGTAATTTCGCTAATTGTATTATATTAAGGTGGTAATAAACAAGAAAGAAAGAAAAAAAAAAGTTGTTAAATCCAAAAGGGGAAAAAGGTGGGTAAAAAGTGTCGGCATAAAACACCATATAATTAATAGTGGAGATTTTTTTCTTTTTTTTCTTTTAAATTTTTGGTGAATGTATGAATAGCAATTTATGTTCCATTCTTTTTTCCTCTAAATTGTATTGTAACTGAATATTATCTGATTTTTTATAATATTAATAATGTATTAGAATGTTAA

mRNA sequence

GTGATGTTTGACATATTTAATTTCATATCTCAAAATTTTAATTAAAGAAAAAAAAAAAAAGAGTGACCAATAAGTTAATAACTTAGATAGTAAGACTTAAAAAAGATGGTCTCTCTCTCTAAAGATAACACAGTTCCAATCTCTCTCCATCTCCAATTGTAAGATAAAACACAGTGGGGAGTGGCCATAGTTTCAAGTAGCAGCCAGCTAATTCTCCCTTCGACGGCGACTTCTCACACCCACCACTCTCTCTCTAACTTCATATATATTTATATATTTTACTTAAAATCACTCAAAATCCCACCTCCACCTTTACCATTTCTCTCTCTCTCTCTTGTTTCTCTCTCCTCTGCAAAATATGGGTTATGGCCGCCTCAACGAAACCGACCCTGGTGGCTCCTCCGGCGCTGTAACTTTCCAGCTCTCCGACGACGCCGTTTCATTCCCCACTCCCTCTCCCAACCGAAGAAGGAGAACACGACTTCTCCTTCTCTCCCTTCTCTCCCTCATTCTCATCATCGCCGCCGCTGTCTCCGCCACTTTCCTCGTCGGACTCCGAACCGCCTCTTCCTCTCCCCGATCCGACCCGAATCTCCCTCGAAAACCCACTCAGGCCATTTCCAAGGCCTGTAGCCGAACTCGCTTCCCGACTCTCTGTGTCAACTCGCTACTCGATTTCCCTGGATCTCTCAACGCCAACGAACACGATCTCGTCCACATTTCCTTCAATTTAACTCTCCAGCATCTCAACAAAGCCCTCTATCTCTCCTCTGAGATTTCCTCTCTTCAAATGGATCTTCGCGTTCGCTCTGCCTATGACGCTTGTCTTGAACTCCTCGACGACTCTGTCGACGCTCTCACACGCTCCTTACAGTCCGTCGCGCCACCTTCCTCTGACGGCACCCCCCATCGCCTCGCCTCCTCCGATGACATCATCACCTGGCTCAGCGCCGCCCTCACCAACCAAGACACCTGTTCCGACGGATTCTCCGAACTTACTGGAAATGTTAAAGATCAAATGACGGATAAACTCCACAATTTATCCGAGCTTGTGAGCAATTGCTTGGCGCTGTTTTCCGGTAGTGAAACGAACGATTTCGCCGGAGTGCCGATTCAGAACAAGCGACGGTTGATGGACGCCGGGGAGAACGTGGTTAGTTCCGGTAACTTCCCCGGCTGGATGAGCCGGAGAGAGAGGCGGTTACTGACGCTGCCGGTGGGGGCGCTACAGGCCGATATTGTGGTTTCTAAGGACGGGAACGGAACGGTGAAGACGATTGCCGAAGCGATCAAAAAGGCTCCGGAATACAGTAGCAAAAGGACGATCATCTATGTGATGGCAGGAAGGTACGAAGAGAAGAATCTGAAGGTGGGAAGGAAGAAAACGAACCTGATGTTTGTTGGGGATGGAAAGGGTAAAACAGTCATTTCGGGTGCTAAGAGTATCTTTGACAACGTGACAACTTTTCACACGGCGTCGTTTGCGGCCACCGGTGCCGGTATAATATTGAGGGACATGACATTTGAGAATTGGGCCGGACCGGGTCGACACCAGGCGGTGGCGCTCCGAGTCGGCGCCGACCACGCCGTCGTGTACCGCTGCAACGTAATCGGCTATCAAGACACTCTGTACGTCCATTCCAACCGCCAATTCTTTAGAGAGTGCGACATTTACGGGACCGTCGATTTCATCTTTGGCAATGCGGCGGTGGTGTTTCAGAACTGCAGCATTTACGCCCGAAAAGCGATGGACCTTCAAAAGAACACAATCACAGCCCAGAATCGGAAGGATCCGAATCAGAATACCGGAATTTCCATCCACGCCTGCCGGATTTTAGCCACCTCCGATCTGTCGTCGTCCAATAGCAGCAACCCGACGTATCTGGGCCGGCCGTGGAAATTATACTCTAGAGTCGTGTACATGCTATCGTACATAGGGAGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAATGCCACGTTTGCACTTGACACGTTGTATTACGGTGAGTATATGAATTATGGGCCGGGTGGGGCAGTGGGCCAGAGGGTGACGTGGCCTGGGTATCGGGTCATAACGTCCCCGGTGGAGGCGAGTAAGTTTACGGTGGCGCAGTTCATTTACGGCTCGTCGTGGTTGCCGTCCACTGGAGTTGCATTTCTTGGAGGGTTAAATGTGTAATTTCGCTAATTGTATTATATTAAGGTGGTAATAAACAAGAAAGAAAGAAAAAAAAAAGTTGTTAAATCCAAAAGGGGAAAAAGGTGGGTAAAAAGTGTCGGCATAAAACACCATATAATTAATAGTGGAGATTTTTTTCTTTTTTTTCTTTTAAATTTTTGGTGAATGTATGAATAGCAATTTATGTTCCATTCTTTTTTCCTCTAAATTGTATTGTAACTGAATATTATCTGATTTTTTATAATATTAATAATGTATTAGAATGTTAA

Coding sequence (CDS)

ATGGGTTATGGCCGCCTCAACGAAACCGACCCTGGTGGCTCCTCCGGCGCTGTAACTTTCCAGCTCTCCGACGACGCCGTTTCATTCCCCACTCCCTCTCCCAACCGAAGAAGGAGAACACGACTTCTCCTTCTCTCCCTTCTCTCCCTCATTCTCATCATCGCCGCCGCTGTCTCCGCCACTTTCCTCGTCGGACTCCGAACCGCCTCTTCCTCTCCCCGATCCGACCCGAATCTCCCTCGAAAACCCACTCAGGCCATTTCCAAGGCCTGTAGCCGAACTCGCTTCCCGACTCTCTGTGTCAACTCGCTACTCGATTTCCCTGGATCTCTCAACGCCAACGAACACGATCTCGTCCACATTTCCTTCAATTTAACTCTCCAGCATCTCAACAAAGCCCTCTATCTCTCCTCTGAGATTTCCTCTCTTCAAATGGATCTTCGCGTTCGCTCTGCCTATGACGCTTGTCTTGAACTCCTCGACGACTCTGTCGACGCTCTCACACGCTCCTTACAGTCCGTCGCGCCACCTTCCTCTGACGGCACCCCCCATCGCCTCGCCTCCTCCGATGACATCATCACCTGGCTCAGCGCCGCCCTCACCAACCAAGACACCTGTTCCGACGGATTCTCCGAACTTACTGGAAATGTTAAAGATCAAATGACGGATAAACTCCACAATTTATCCGAGCTTGTGAGCAATTGCTTGGCGCTGTTTTCCGGTAGTGAAACGAACGATTTCGCCGGAGTGCCGATTCAGAACAAGCGACGGTTGATGGACGCCGGGGAGAACGTGGTTAGTTCCGGTAACTTCCCCGGCTGGATGAGCCGGAGAGAGAGGCGGTTACTGACGCTGCCGGTGGGGGCGCTACAGGCCGATATTGTGGTTTCTAAGGACGGGAACGGAACGGTGAAGACGATTGCCGAAGCGATCAAAAAGGCTCCGGAATACAGTAGCAAAAGGACGATCATCTATGTGATGGCAGGAAGGTACGAAGAGAAGAATCTGAAGGTGGGAAGGAAGAAAACGAACCTGATGTTTGTTGGGGATGGAAAGGGTAAAACAGTCATTTCGGGTGCTAAGAGTATCTTTGACAACGTGACAACTTTTCACACGGCGTCGTTTGCGGCCACCGGTGCCGGTATAATATTGAGGGACATGACATTTGAGAATTGGGCCGGACCGGGTCGACACCAGGCGGTGGCGCTCCGAGTCGGCGCCGACCACGCCGTCGTGTACCGCTGCAACGTAATCGGCTATCAAGACACTCTGTACGTCCATTCCAACCGCCAATTCTTTAGAGAGTGCGACATTTACGGGACCGTCGATTTCATCTTTGGCAATGCGGCGGTGGTGTTTCAGAACTGCAGCATTTACGCCCGAAAAGCGATGGACCTTCAAAAGAACACAATCACAGCCCAGAATCGGAAGGATCCGAATCAGAATACCGGAATTTCCATCCACGCCTGCCGGATTTTAGCCACCTCCGATCTGTCGTCGTCCAATAGCAGCAACCCGACGTATCTGGGCCGGCCGTGGAAATTATACTCTAGAGTCGTGTACATGCTATCGTACATAGGGAGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAATGCCACGTTTGCACTTGACACGTTGTATTACGGTGAGTATATGAATTATGGGCCGGGTGGGGCAGTGGGCCAGAGGGTGACGTGGCCTGGGTATCGGGTCATAACGTCCCCGGTGGAGGCGAGTAAGTTTACGGTGGCGCAGTTCATTTACGGCTCGTCGTGGTTGCCGTCCACTGGAGTTGCATTTCTTGGAGGGTTAAATGTGTAA

Protein sequence

MGYGRLNETDPGGSSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAAAVSATFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEHDLVHISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDGTPHRLASSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCLALFSGSETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV

Homology

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BLAST of Tan0016494 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 4.9e-228
Identity = 407/615 (66.18%), Postives = 477/615 (77.56%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQ-----LSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIA 60
           MGY RL    P G++G+VT       + +   +   P  N RR  + L++S  S++L I+
Sbjct: 1   MGYERLG---PSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVS--SIVLAIS 60

Query: 61  AAVSATFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNA-N 120
             ++A    G+R+     +S P L RKP+QAISKAC  TRFP LCV+SL+DFPGSL A +
Sbjct: 61  LILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASS 120

Query: 121 EHDLVHISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSV 180
             DL+H++ N+TL H + ALY S+ +S + M  R RSAYD+C+ELLDDSVDAL+R+L SV
Sbjct: 121 SKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSV 180

Query: 181 APPSSDGTPHRLASSDDIITWLSAALTNQDTCSDGFSEL-TGNVKDQMTDKLHNLSELVS 240
              S        A   D+ TWLSAALTN DTC++GF  +  G VKD MT  L NLSELVS
Sbjct: 181 VSSS--------AKPQDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVS 240

Query: 241 NCLALFSGS-ETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQA 300
           NCLA+FS S + +DFAGVPIQN RRL+   E       FP WM  +ER +L +PV  +QA
Sbjct: 241 NCLAIFSASHDGDDFAGVPIQN-RRLLGVEER---EEKFPRWMRPKEREILEMPVSQIQA 300

Query: 301 DIVVSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGK 360
           DI+VSKDGNGT KTI+EAIKKAP+ S++R IIYV AGRYEE NLKVGRKK NLMFVGDGK
Sbjct: 301 DIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGK 360

Query: 361 GKTVISGAKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVV 420
           GKTVISG KSIFDN+TTFHTASFAATGAG I RD+TFENWAGP +HQAVALR+GADHAV+
Sbjct: 361 GKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVI 420

Query: 421 YRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTIT 480
           YRCN+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVV QNCSIYARK MD QKNTIT
Sbjct: 421 YRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTIT 480

Query: 481 AQNRKDPNQNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHP 540
           AQNRKDPNQNTGISIHA R+LA SDL ++N S  TYLGRPWKL+SR VYM+SYIG HVH 
Sbjct: 481 AQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHT 540

Query: 541 RGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSS 600
           RGWLEWN TFALDTLYYGEY+N GPG  +GQRV+WPGYRVI S  EA++FTVA+FIYGSS
Sbjct: 541 RGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSS 598

Query: 601 WLPSTGVAFLGGLNV 608
           WLPSTGV+FL GL++
Sbjct: 601 WLPSTGVSFLAGLSI 598

[Close]

BLAST of Tan0016494 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 1.9e-219
Identity = 391/609 (64.20%), Postives = 468/609 (76.85%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAAAVSA 60
           MGY RL      G SG       D A S   P   ++ +T+L+   L +L +++   V  
Sbjct: 1   MGYDRL------GPSGPSNPNQKDPATSL--PELQKKTKTKLI---LFTLAVLVVGVVCF 60

Query: 61  TFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEHDLVH 120
               G+R A  S +++P L RKPTQAIS+ CS++ +P LC+++LLDFPGSL A+E++L+H
Sbjct: 61  GIFAGIR-AVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIH 120

Query: 121 ISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN TLQ  +KALY SS I+  QM  RVRSAYD+CLELLDDSVDALTR+L SV   S D
Sbjct: 121 ISFNATLQKFSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGD 180

Query: 181 GTPHRLASSDDIITWLSAALTNQDTCSDGFSELT---GNVKDQMTDKLHNLSELVSNCLA 240
                  S  D++TWLS+A+TN DTC+DGF E+    G VKDQ+   + +LSE+VSNCLA
Sbjct: 181 ------ESHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLA 240

Query: 241 LFSGSETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVS 300
           +F+G +  D +GVP+ N R+L+   E    +   P W+ R +R LL  P  A+QADI VS
Sbjct: 241 IFAG-KVKDLSGVPVVNNRKLLGTEE----TEELPNWLKREDRELLGTPTSAIQADITVS 300

Query: 301 KDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVI 360
           KDG+GT KTIAEAIKKAPE+SS+R +IYV AGRYEE+NLKVGRKKTNLMF+GDGKGKTVI
Sbjct: 301 KDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVI 360

Query: 361 SGAKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNV 420
           +G KSI D++TTFHTA+FAATGAG I+RDMTFEN+AGP +HQAVALRVG DHAVVYRCN+
Sbjct: 361 TGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNI 420

Query: 421 IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRK 480
           IGYQD LYVHSNRQFFREC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK TITAQNRK
Sbjct: 421 IGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRK 480

Query: 481 DPNQNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLE 540
           DPNQNTGISIHAC++LAT DL +S  S PTYLGRPWKLYSRVVYM+S +G H+ PRGWLE
Sbjct: 481 DPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLE 540

Query: 541 WNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPST 600
           WN  FALD+LYYGEYMN G G  +GQRV WPGY VITS VEASKFTVAQFI GSSWLPST
Sbjct: 541 WNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPST 586

Query: 601 GVAFLGGLN 607
           GV+F  GL+
Sbjct: 601 GVSFFSGLS 586

[Close]

BLAST of Tan0016494 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 4.7e-122
Identity = 247/579 (42.66%), Postives = 353/579 (60.97%), Query Frame = 0


Query: 35  NRRRRTRLLLLSLLSLILIIAAAVSATFLVGLRTASSSPRSDPNLPRKPTQAISK-ACSR 94
           N  ++ + L L+L + +L++AA      ++G+    +S ++  +   +P  AI K +CS 
Sbjct: 22  NIPKKKKKLFLALFATLLVVAA------VIGIVAGVNSRKNSGDNGNEPHHAILKSSCSS 81

Query: 95  TRFPTLCVNSLLDFPGSLN--ANEHDLVHISFNLTLQHLNKALY-LSSEISSLQMDLRVR 154
           TR+P LC +++   P +     ++ D++ +S N+T   +    + +   +    +  R +
Sbjct: 82  TRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREK 141

Query: 155 SAYDACLELLDDSVDALTRSLQSVA--PPSSDGTPHRLASSDDIITWLSAALTNQDTCSD 214
            A   CLE +D+++D L ++++ +   P     + H    +DD+ T +SAA+TNQ TC D
Sbjct: 142 VALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQH----ADDLKTLMSAAMTNQGTCLD 201

Query: 215 GFSELTGN--VKDQMTDKLHNLSELVSNCLALFSGSETNDFAGVPIQNKRRLMDAGENVV 274
           GFS    N  V+D ++D   ++ ++ SN LA+       D   +   N R+L++    V 
Sbjct: 202 GFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEETSTV- 261

Query: 275 SSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEYSSKRTIIYV 334
               +P W+S  +RRL  L   ++  ++VV+ DG+G  KT+A ++  AP+  +KR II +
Sbjct: 262 --DGWPAWLSTGDRRL--LQSSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRI 321

Query: 335 MAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTASFAATGAGIILRD 394
            AG Y E N++V +K  N+MF+GDG+ +T+I+G++++ D  TTF +A+ A  G G + RD
Sbjct: 322 KAGVYRE-NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARD 381

Query: 395 MTFENWAGPGRHQAVALRVGADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIF 454
           +TF+N AGP +HQAVALRVGAD +  Y C+++ YQDTLYVHSNRQFF  C I GTVDFIF
Sbjct: 382 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 441

Query: 455 GNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILATSDLSSSNSSNP 514
           GNAA V QNC I+ARK    QKN +TAQ R DPNQNTGI I   RI ATSDL     S P
Sbjct: 442 GNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFP 501

Query: 515 TYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVT 574
           TYLGRPWK YSR V M S I   +HP GW EW+  FAL+TL+YGE+ N G G     RV 
Sbjct: 502 TYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVK 561

Query: 575 WPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVAFLGGL 606
           W G+RVITS  EA  FT   FI GSSWL STG  F  GL
Sbjct: 562 WKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of Tan0016494 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 439.1 bits (1128), Expect = 8.1e-122
Identity = 247/591 (41.79%), Postives = 349/591 (59.05%), Query Frame = 0


Query: 35  NRRRRTRLLLLSLLSLILIIAAAVSATFLVGLRTASSSPRSDPNLPRKPTQAISKACSRT 94
           N ++  +L+LLS    +L +AA      + G+   +S       L       +  +CS T
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAA------VAGISAGASKANEKRTLSPSSHAVLRSSCSST 72

Query: 95  RFPTLCVNSLLDFPGSLNANEHDLVHISFNLTLQHLNKALYLSSEISSLQMDL--RVRSA 154
           R+P LC+++++   G    ++ D++  S NLT+  +    +   ++   +  L  R ++A
Sbjct: 73  RYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 132

Query: 155 YDACLELLDDSVDALTRSLQSVAPPSSDGTPHRLASSDDIITWLSAALTNQDTCSDGFS- 214
              CLE +D+++D L  +++ +    +  T    A   D+ T +S+A+TNQ+TC DGFS 
Sbjct: 133 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAG--DLKTLISSAITNQETCLDGFSH 192

Query: 215 -ELTGNVKDQMTDKLHNLSELVSNCLALFSGSETNDFAGVP-----IQNKRRLMD----- 274
            +    V+  +     ++  + SN LA+       D A          N R+L +     
Sbjct: 193 DDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQET 252

Query: 275 ------AGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKA 334
                 AG   + S  +P W+S  +RRL  L    ++AD  V+ DG+GT KT+A A+  A
Sbjct: 253 TVAVDIAGAGELDSEGWPTWLSAGDRRL--LQGSGVKADATVAADGSGTFKTVAAAVAAA 312

Query: 335 PEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTAS 394
           PE S+KR +I++ AG Y E N++V +KK N+MF+GDG+ +T+I+G++++ D  TTFH+A+
Sbjct: 313 PENSNKRYVIHIKAGVYRE-NVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSAT 372

Query: 395 FAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGYQDTLYVHSNRQFFR 454
            AA G   + RD+TF+N AGP +HQAVALRVG+D +  Y C+++ YQDTLYVHSNRQFF 
Sbjct: 373 VAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFV 432

Query: 455 ECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILA 514
           +C I GTVDFIFGNAAVV Q+C I+AR+    QKN +TAQ R DPNQNTGI I  CRI A
Sbjct: 433 KCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGA 492

Query: 515 TSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMN 574
           TSDL S   S PTYLGRPWK YS+ V M S I   + P GW EW  TFAL+TL Y EY N
Sbjct: 493 TSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSN 552

Query: 575 YGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVAFLGGL 606
            G G     RV W G++VIT+  EA K+T  QFI G  WL STG  F  GL
Sbjct: 553 TGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of Tan0016494 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.8e-121
Identity = 248/579 (42.83%), Postives = 351/579 (60.62%), Query Frame = 0


Query: 35  NRRRRTRLLLLSLLSLILIIAAAVSATFLVGLRTASSSPRSDPNLPRKPTQAISK-ACSR 94
           N  ++ + L L+L + +L++AA      ++G+    +S ++  +   +P  AI K +CS 
Sbjct: 22  NIPKKKKKLFLALFATLLVVAA------VIGIVAGVNSRKNSGDNGNEPHHAILKSSCSS 81

Query: 95  TRFPTLCVNSLLDFPGSLN--ANEHDLVHISFNLTLQHLNKALY-LSSEISSLQMDLRVR 154
           TR+P LC +++   P +     ++ D++ +S N+T   +    + +   +    +  R +
Sbjct: 82  TRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREK 141

Query: 155 SAYDACLELLDDSVDALTRSLQSVA--PPSSDGTPHRLASSDDIITWLSAALTNQDTCSD 214
            A   CLE +D+++D L ++++ +   P     + H    +DD+ T +SAA+TNQ TC D
Sbjct: 142 VALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQH----ADDLKTLMSAAMTNQGTCLD 201

Query: 215 GFSELTGN--VKDQMTDKLHNLSELVSNCLALFSGSETNDFAGVPIQNKRRLMDAGENVV 274
           GFS    N  V+D ++D   ++ ++ SN LA+       D   +   N R+L +    V 
Sbjct: 202 GFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTEETSTV- 261

Query: 275 SSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEYSSKRTIIYV 334
               +P W+S  +RRL  L   ++  + VV+ DG+G  KT+A A+  AP+  +KR II +
Sbjct: 262 --DGWPAWLSPGDRRL--LQSSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRI 321

Query: 335 MAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTASFAATGAGIILRD 394
            AG Y E N++V +K  N+MF+GDG+ +T+I+G++++ D  TTF +A+ A  G G + RD
Sbjct: 322 KAGVYRE-NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARD 381

Query: 395 MTFENWAGPGRHQAVALRVGADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIF 454
           +TF+N AGP +HQAVALRVGAD +  Y C+++ YQDTLYVHSNRQFF  C I GTVDFIF
Sbjct: 382 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 441

Query: 455 GNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILATSDLSSSNSSNP 514
           GNAA V QNC I+ARK    QKN +TAQ R DPNQNTGI I   RI ATSDL     S P
Sbjct: 442 GNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFP 501

Query: 515 TYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVT 574
           TYLGRPWK YSR V M S I   +HP GW EW+  FAL+TL+YGE+ N G G     RV 
Sbjct: 502 TYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVK 561

Query: 575 WPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVAFLGGL 606
           W G+RVITS  EA  FT   FI GSSWL STG  F  GL
Sbjct: 562 WKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of Tan0016494 vs. NCBI nr
Match: XP_038881267.1 (probable pectinesterase/pectinesterase inhibitor 34 [Benincasa hispida])

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 553/607 (91.10%), Postives = 582/607 (95.88%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAAAVSA 60
           MGYGRLNE+DPGG+S A  FQLSD  +SFPTPS   R R +L L+SLLSL+LI+AAAVSA
Sbjct: 1   MGYGRLNESDPGGTSSA-AFQLSDHPISFPTPS---RSRAQLFLISLLSLLLIVAAAVSA 60

Query: 61  TFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEHDLVH 120
           TFL+G+RTAS + RSD NLPRKPTQAISKACSRTRFP+LCVNSLLDFPGSLNANE DLVH
Sbjct: 61  TFLIGIRTASPA-RSDRNLPRKPTQAISKACSRTRFPSLCVNSLLDFPGSLNANEQDLVH 120

Query: 121 ISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN+TLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP SS 
Sbjct: 121 ISFNITLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSS 180

Query: 181 GTPHRLASSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCLALFS 240
           GTP RL SS+D+ITWLSAALTNQDTC+DGFSEL+G VK+QM DKLHNLSELVSNCLALFS
Sbjct: 181 GTPQRLGSSEDVITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFS 240

Query: 241 GSETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDG 300
           GSET+DF+GVPIQNKRRLM+AG+N  +SGNFPGWMSRRERRLLTLPVGALQADIVVS++G
Sbjct: 241 GSETSDFSGVPIQNKRRLMNAGDNEDTSGNFPGWMSRRERRLLTLPVGALQADIVVSQNG 300

Query: 301 NGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGA 360
           NGTVKTIAEAIKKAP+YSS+RTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGA
Sbjct: 301 NGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGA 360

Query: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGY 420
           KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCN+IGY
Sbjct: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGY 420

Query: 421 QDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN 480
           QDTLYVHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQNRKDPN
Sbjct: 421 QDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDPN 480

Query: 481 QNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNA 540
           QNTGISIHACRILATSDL+SSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNA
Sbjct: 481 QNTGISIHACRILATSDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNA 540

Query: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVA 600
           TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEASKFTVAQFIYGSSWLPSTGVA
Sbjct: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVA 600

Query: 601 FLGGLNV 608
           FLGGLNV
Sbjct: 601 FLGGLNV 602

[Close]

BLAST of Tan0016494 vs. NCBI nr
Match: XP_022922767.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita moschata])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 547/607 (90.12%), Postives = 574/607 (94.56%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAAAVSA 60
           MGYGRLNETDPGG+S      LS  A S   P+P   RR+RLLL+SLLSLILI+AAAVSA
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAAS---PNP---RRSRLLLISLLSLILIVAAAVSA 60

Query: 61  TFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEHDLVH 120
           TFLVGLRTA SS RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANE DLVH
Sbjct: 61  TFLVGLRTA-SSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVH 120

Query: 121 ISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN+TLQHLNKALY SS ISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP SS+
Sbjct: 121 ISFNMTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSN 180

Query: 181 GTPHRLASSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCLALFS 240
           G P RL SSDD+ITWLSAALTNQDTC+DGFS+L+G+VKDQM DKLHNLSELVSNCLALFS
Sbjct: 181 GGPQRLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFS 240

Query: 241 GSETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDG 300
           GS T+DF+GVPIQNKRRLM+AGEN  +SGNFPGWMSRRERRLLTLPVGALQADIVVS++G
Sbjct: 241 GSVTSDFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNG 300

Query: 301 NGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGA 360
           +GTVKTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG 
Sbjct: 301 SGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGG 360

Query: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGY 420
           KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVG DHAVVYRCN+IGY
Sbjct: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGY 420

Query: 421 QDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN 480
           QDTLYVHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNT+TAQNRKDPN
Sbjct: 421 QDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPN 480

Query: 481 QNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNA 540
           QNTGISIHACRILA SDL+SSNSSNPTYLGRPWKLYSRVV+MLSYIGSHVHPRGWLEWNA
Sbjct: 481 QNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNA 540

Query: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVA 600
           TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEASKFTVAQFIYGSSWLPSTGVA
Sbjct: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVA 600

Query: 601 FLGGLNV 608
           FLGGLNV
Sbjct: 601 FLGGLNV 600

[Close]

BLAST of Tan0016494 vs. NCBI nr
Match: XP_022984620.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita maxima])

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 547/607 (90.12%), Postives = 574/607 (94.56%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAAAVSA 60
           M YGRLN+TDPGG+S     QLS  AV     SPN RRR RL+L+SLLSLILI+AAAVSA
Sbjct: 1   MAYGRLNQTDPGGTSSDAPSQLSHHAV-----SPNPRRR-RLILISLLSLILIVAAAVSA 60

Query: 61  TFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEHDLVH 120
           TFLVGLRTA SS RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANE DLVH
Sbjct: 61  TFLVGLRTA-SSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVH 120

Query: 121 ISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN+TLQHLNKALY SS ISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP SS+
Sbjct: 121 ISFNMTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSN 180

Query: 181 GTPHRLASSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCLALFS 240
           G P RL SSDD+ITWLSAALTNQDTC+DGFS+L+G+VKDQM DKLHNLSELVSNCLALFS
Sbjct: 181 GGPQRLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFS 240

Query: 241 GSETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDG 300
           GS T+DF+GVPIQNKRRLM+AGEN  +SG+FPGWMSRRERRLLTLPVGALQADIVVS++G
Sbjct: 241 GSVTSDFSGVPIQNKRRLMNAGENEDTSGSFPGWMSRRERRLLTLPVGALQADIVVSQNG 300

Query: 301 NGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGA 360
           +GTVKTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG 
Sbjct: 301 SGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKCNLMFVGDGKGKTVISGG 360

Query: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGY 420
           KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVG DHAVVYRCN+IGY
Sbjct: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGY 420

Query: 421 QDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN 480
           QDTLYVHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN
Sbjct: 421 QDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN 480

Query: 481 QNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNA 540
           QNTGISIHACRILA SDL+SSNSSNPTYLGRPWKLYSRVV+MLSYIGSHVHPRGWLEWNA
Sbjct: 481 QNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNA 540

Query: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVA 600
           TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEASKFTVAQFIYGSSWLPSTGVA
Sbjct: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVA 600

Query: 601 FLGGLNV 608
           FLGGLNV
Sbjct: 601 FLGGLNV 600

[Close]

BLAST of Tan0016494 vs. NCBI nr
Match: XP_023553011.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 546/607 (89.95%), Postives = 573/607 (94.40%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAAAVSA 60
           MGYGRLNETDPGG+S      LS  A      SPN RRR RLLL+SLLSLILI+ AAVSA
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAA-----SPNPRRR-RLLLISLLSLILIVTAAVSA 60

Query: 61  TFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEHDLVH 120
           TFLVGLRTA SS RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANE DLVH
Sbjct: 61  TFLVGLRTA-SSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVH 120

Query: 121 ISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN+TLQHLNKALY SSEISSLQMDLRVRSAYDAC+ELLDDSVDALTRSLQSVAP SS+
Sbjct: 121 ISFNITLQHLNKALYFSSEISSLQMDLRVRSAYDACVELLDDSVDALTRSLQSVAPSSSN 180

Query: 181 GTPHRLASSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCLALFS 240
           G P RL SSDD+ITWLSAALTNQDTC+DGFS+L+G+VKDQM DKLHNLSELVSNCLALFS
Sbjct: 181 GGPQRLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFS 240

Query: 241 GSETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDG 300
           GS T+DF+GVPIQNKRRLM+AG++  +SGNFPGWMSRRERRLLTLPVGALQADIVVS++G
Sbjct: 241 GSVTSDFSGVPIQNKRRLMNAGDDEDTSGNFPGWMSRRERRLLTLPVGALQADIVVSQNG 300

Query: 301 NGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGA 360
           +GTVKTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG 
Sbjct: 301 SGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGG 360

Query: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGY 420
           KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVG DHAVVYRCN+IGY
Sbjct: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGY 420

Query: 421 QDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN 480
           QDTLYVHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN
Sbjct: 421 QDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN 480

Query: 481 QNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNA 540
           QNTGISIHACRILA SDL+SSNSSNPTYLGRPWKLYSRVV+MLSYIGSHVHPRGWLEWNA
Sbjct: 481 QNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNA 540

Query: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVA 600
           TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEASKFTVAQFIYGSSWLPSTGVA
Sbjct: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVA 600

Query: 601 FLGGLNV 608
           FLGGLNV
Sbjct: 601 FLGGLNV 600

[Close]

BLAST of Tan0016494 vs. NCBI nr
Match: KAA0050503.1 (pectin methylesterase family protein [Cucumis melo var. makuwa])

HSP 1 Score: 1064.7 bits (2752), Expect = 3.0e-307
Identity = 544/612 (88.89%), Postives = 570/612 (93.14%), Query Frame = 0


Query: 1   MGYGRLNETDPGG----SSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAA 60
           MGYGRLNETDPGG    SS A  FQLS      PTP+   R  TRLLL+S LSL+LI+AA
Sbjct: 1   MGYGRLNETDPGGPSDSSSVASAFQLS---AHHPTPN---RSTTRLLLISFLSLLLIVAA 60

Query: 61  AVSATFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEH 120
           AVSATFL+GLRTAS + RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANE 
Sbjct: 61  AVSATFLIGLRTASPA-RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQ 120

Query: 121 DLVHISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP 180
           DLVHISFN+TLQHLNKALY SSEISSLQMDLRVRSAYDACLELLDDS+DA++RSLQSVAP
Sbjct: 121 DLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAP 180

Query: 181 PSSDGTPHRLASSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCL 240
            SS  TP RL SS+DIITWLSAALTNQDTC+DGFSEL+G VK+QM DKLHNLSELVSNCL
Sbjct: 181 SSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCL 240

Query: 241 ALFSGSETNDFAGVPIQNKRRLM-DAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIV 300
           ALFSGSET+DFAGVPIQNKRRLM + GEN  SSGNFP WM  RERRLLTLPVG LQADIV
Sbjct: 241 ALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFPRWMRGRERRLLTLPVGVLQADIV 300

Query: 301 VSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT 360
           VS++GNGTVKTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT
Sbjct: 301 VSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT 360

Query: 361 VISGAKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRC 420
           VISG+KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRC
Sbjct: 361 VISGSKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRC 420

Query: 421 NVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQN 480
           N+IGYQDTLYVHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQN
Sbjct: 421 NIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQN 480

Query: 481 RKDPNQNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGW 540
           RKDPNQNTGISIHACRILATSDL SSN+SNPTYLGRPWKLYSRVV+MLSYIG HVHPRGW
Sbjct: 481 RKDPNQNTGISIHACRILATSDLQSSNTSNPTYLGRPWKLYSRVVFMLSYIGGHVHPRGW 540

Query: 541 LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLP 600
           LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEASKFTVAQFIYGSSWLP
Sbjct: 541 LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLP 600

Query: 601 STGVAFLGGLNV 608
           STGVAFLGGLNV
Sbjct: 601 STGVAFLGGLNV 605

[Close]

BLAST of Tan0016494 vs. ExPASy TrEMBL
Match: A0A6J1E487 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 547/607 (90.12%), Postives = 574/607 (94.56%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAAAVSA 60
           MGYGRLNETDPGG+S      LS  A S   P+P   RR+RLLL+SLLSLILI+AAAVSA
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAAS---PNP---RRSRLLLISLLSLILIVAAAVSA 60

Query: 61  TFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEHDLVH 120
           TFLVGLRTA SS RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANE DLVH
Sbjct: 61  TFLVGLRTA-SSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVH 120

Query: 121 ISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN+TLQHLNKALY SS ISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP SS+
Sbjct: 121 ISFNMTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSN 180

Query: 181 GTPHRLASSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCLALFS 240
           G P RL SSDD+ITWLSAALTNQDTC+DGFS+L+G+VKDQM DKLHNLSELVSNCLALFS
Sbjct: 181 GGPQRLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFS 240

Query: 241 GSETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDG 300
           GS T+DF+GVPIQNKRRLM+AGEN  +SGNFPGWMSRRERRLLTLPVGALQADIVVS++G
Sbjct: 241 GSVTSDFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNG 300

Query: 301 NGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGA 360
           +GTVKTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG 
Sbjct: 301 SGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGG 360

Query: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGY 420
           KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVG DHAVVYRCN+IGY
Sbjct: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGY 420

Query: 421 QDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN 480
           QDTLYVHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNT+TAQNRKDPN
Sbjct: 421 QDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPN 480

Query: 481 QNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNA 540
           QNTGISIHACRILA SDL+SSNSSNPTYLGRPWKLYSRVV+MLSYIGSHVHPRGWLEWNA
Sbjct: 481 QNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNA 540

Query: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVA 600
           TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEASKFTVAQFIYGSSWLPSTGVA
Sbjct: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVA 600

Query: 601 FLGGLNV 608
           FLGGLNV
Sbjct: 601 FLGGLNV 600

[Close]

BLAST of Tan0016494 vs. ExPASy TrEMBL
Match: A0A6J1J930 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111482853 PE=3 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 547/607 (90.12%), Postives = 574/607 (94.56%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAAAVSA 60
           M YGRLN+TDPGG+S     QLS  AV     SPN RRR RL+L+SLLSLILI+AAAVSA
Sbjct: 1   MAYGRLNQTDPGGTSSDAPSQLSHHAV-----SPNPRRR-RLILISLLSLILIVAAAVSA 60

Query: 61  TFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEHDLVH 120
           TFLVGLRTA SS RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANE DLVH
Sbjct: 61  TFLVGLRTA-SSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVH 120

Query: 121 ISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN+TLQHLNKALY SS ISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP SS+
Sbjct: 121 ISFNMTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSN 180

Query: 181 GTPHRLASSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCLALFS 240
           G P RL SSDD+ITWLSAALTNQDTC+DGFS+L+G+VKDQM DKLHNLSELVSNCLALFS
Sbjct: 181 GGPQRLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFS 240

Query: 241 GSETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDG 300
           GS T+DF+GVPIQNKRRLM+AGEN  +SG+FPGWMSRRERRLLTLPVGALQADIVVS++G
Sbjct: 241 GSVTSDFSGVPIQNKRRLMNAGENEDTSGSFPGWMSRRERRLLTLPVGALQADIVVSQNG 300

Query: 301 NGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGA 360
           +GTVKTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG 
Sbjct: 301 SGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKCNLMFVGDGKGKTVISGG 360

Query: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGY 420
           KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVG DHAVVYRCN+IGY
Sbjct: 361 KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGY 420

Query: 421 QDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN 480
           QDTLYVHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN
Sbjct: 421 QDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPN 480

Query: 481 QNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNA 540
           QNTGISIHACRILA SDL+SSNSSNPTYLGRPWKLYSRVV+MLSYIGSHVHPRGWLEWNA
Sbjct: 481 QNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNA 540

Query: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVA 600
           TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEASKFTVAQFIYGSSWLPSTGVA
Sbjct: 541 TFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVA 600

Query: 601 FLGGLNV 608
           FLGGLNV
Sbjct: 601 FLGGLNV 600

[Close]

BLAST of Tan0016494 vs. ExPASy TrEMBL
Match: A0A5A7U3N7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001450 PE=3 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 1.4e-307
Identity = 544/612 (88.89%), Postives = 570/612 (93.14%), Query Frame = 0


Query: 1   MGYGRLNETDPGG----SSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAA 60
           MGYGRLNETDPGG    SS A  FQLS      PTP+   R  TRLLL+S LSL+LI+AA
Sbjct: 1   MGYGRLNETDPGGPSDSSSVASAFQLS---AHHPTPN---RSTTRLLLISFLSLLLIVAA 60

Query: 61  AVSATFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEH 120
           AVSATFL+GLRTAS + RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANE 
Sbjct: 61  AVSATFLIGLRTASPA-RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQ 120

Query: 121 DLVHISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP 180
           DLVHISFN+TLQHLNKALY SSEISSLQMDLRVRSAYDACLELLDDS+DA++RSLQSVAP
Sbjct: 121 DLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAP 180

Query: 181 PSSDGTPHRLASSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCL 240
            SS  TP RL SS+DIITWLSAALTNQDTC+DGFSEL+G VK+QM DKLHNLSELVSNCL
Sbjct: 181 SSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCL 240

Query: 241 ALFSGSETNDFAGVPIQNKRRLM-DAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIV 300
           ALFSGSET+DFAGVPIQNKRRLM + GEN  SSGNFP WM  RERRLLTLPVG LQADIV
Sbjct: 241 ALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFPRWMRGRERRLLTLPVGVLQADIV 300

Query: 301 VSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT 360
           VS++GNGTVKTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT
Sbjct: 301 VSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT 360

Query: 361 VISGAKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRC 420
           VISG+KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRC
Sbjct: 361 VISGSKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRC 420

Query: 421 NVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQN 480
           N+IGYQDTLYVHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQN
Sbjct: 421 NIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQN 480

Query: 481 RKDPNQNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGW 540
           RKDPNQNTGISIHACRILATSDL SSN+SNPTYLGRPWKLYSRVV+MLSYIG HVHPRGW
Sbjct: 481 RKDPNQNTGISIHACRILATSDLQSSNTSNPTYLGRPWKLYSRVVFMLSYIGGHVHPRGW 540

Query: 541 LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLP 600
           LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEASKFTVAQFIYGSSWLP
Sbjct: 541 LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLP 600

Query: 601 STGVAFLGGLNV 608
           STGVAFLGGLNV
Sbjct: 601 STGVAFLGGLNV 605

[Close]

BLAST of Tan0016494 vs. ExPASy TrEMBL
Match: A0A0A0L6S0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G239880 PE=3 SV=1)

HSP 1 Score: 1063.9 bits (2750), Expect = 2.5e-307
Identity = 543/612 (88.73%), Postives = 570/612 (93.14%), Query Frame = 0


Query: 1   MGYGRLNETDPGG----SSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAA 60
           MGYGRLNETDPGG    S  +  FQLS        P+PN RR TRLLL+S LSL+LI+AA
Sbjct: 1   MGYGRLNETDPGGPSHSSPASPPFQLSSH-----HPTPN-RRTTRLLLISFLSLLLIVAA 60

Query: 61  AVSATFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEH 120
           AVSATFL+GLRTAS + RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANE 
Sbjct: 61  AVSATFLIGLRTASPA-RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQ 120

Query: 121 DLVHISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP 180
           DLVHISFN+TLQHLNKALY SSEISSLQMDLRVRSAYDACLELLDDS+DA++RSLQSVAP
Sbjct: 121 DLVHISFNVTLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAP 180

Query: 181 PSSDGTPHRLASSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCL 240
            SS  TP RL SS+DIITWLSAALTNQDTC+DGFSEL+G VK+QM DKLHNLSELVSNCL
Sbjct: 181 SSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCL 240

Query: 241 ALFSGSETNDFAGVPIQNKRRLM-DAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIV 300
           ALFSGSET+DFAGVPIQNKRRLM + GEN  SSG FP WM+RRERRLLTLPVG LQADIV
Sbjct: 241 ALFSGSETSDFAGVPIQNKRRLMEEEGENEDSSGKFPRWMNRRERRLLTLPVGVLQADIV 300

Query: 301 VSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT 360
           VS++GNGTVKTIAEAIKKAP+YSS+RTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT
Sbjct: 301 VSQNGNGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKT 360

Query: 361 VISGAKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRC 420
           VISG+KSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRC
Sbjct: 361 VISGSKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRC 420

Query: 421 NVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQN 480
           N+IGYQDTLYVHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQN
Sbjct: 421 NIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQN 480

Query: 481 RKDPNQNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGW 540
           RKDPNQNTGISIHACRILATSDL SSN+SNPTYLGRPWKLYSR V MLSYIG+HVHPRGW
Sbjct: 481 RKDPNQNTGISIHACRILATSDLESSNTSNPTYLGRPWKLYSRTVVMLSYIGNHVHPRGW 540

Query: 541 LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLP 600
           LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEASKFTVAQFIYGSSWLP
Sbjct: 541 LEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLP 600

Query: 601 STGVAFLGGLNV 608
           STGVAFLGGLNV
Sbjct: 601 STGVAFLGGLNV 605

[Close]

BLAST of Tan0016494 vs. ExPASy TrEMBL
Match: A0A6J1G0F6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449561 PE=3 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 7.5e-280
Identity = 503/609 (82.59%), Postives = 534/609 (87.68%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAAAVSA 60
           MG  RLNET P  +                       RR  L +LSLLSLILI+  AVS 
Sbjct: 1   MGCRRLNETHPAANP----------------------RRGPLSVLSLLSLILIL-PAVSP 60

Query: 61  TFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEHDLVH 120
           T L+GLRT+     SDP LPRKPT+AISKAC+R+R P LCV+SLLDFPGSL+ANE DLVH
Sbjct: 61  TSLLGLRTS-----SDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVH 120

Query: 121 ISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFNLTL HLN+ALY SS +SSL MDL +RSAYDACLELLDDSV ALTRS  S+APPSSD
Sbjct: 121 ISFNLTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSD 180

Query: 181 GTPHRLA-SSDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCLALF 240
             P  LA SSDD+ITWLSAALTNQDTC+DGFS+L+GNVKDQMTDKLHNLSELVSNCLALF
Sbjct: 181 SRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALF 240

Query: 241 SGSETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKD 300
           SGS  NDF+GVPIQNKRRLM+A E    SGNFPGWMSRRERRLLTLPVGALQADIVVSKD
Sbjct: 241 SGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKD 300

Query: 301 GNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360
           GNGTVKTIAEAIKKAPE+S+++TIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG
Sbjct: 301 GNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360

Query: 361 AKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIG 420
            KSIFDNVTTFHTASFA +GAGIILRDMTF NWAGP RHQAVALRV ADHAVVYRCNVIG
Sbjct: 361 GKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIG 420

Query: 421 YQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDP 480
           YQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAM+LQKNTITAQNRKDP
Sbjct: 421 YQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDP 480

Query: 481 NQNTGISIHACRILATSDL-SSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEW 540
           NQNTGISIHACRILAT DL SSS ++NPTYLGRPWKLYSRVVYM SY+G HVHPRGWLEW
Sbjct: 481 NQNTGISIHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEW 540

Query: 541 NATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTG 600
           NATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEAS+FTVAQFIYGSSWLPSTG
Sbjct: 541 NATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTG 581

Query: 601 VAFLGGLNV 608
           VAFLGGLN+
Sbjct: 601 VAFLGGLNL 581

[Close]

BLAST of Tan0016494 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 792.0 bits (2044), Expect = 3.5e-229
Identity = 407/615 (66.18%), Postives = 477/615 (77.56%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQ-----LSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIA 60
           MGY RL    P G++G+VT       + +   +   P  N RR  + L++S  S++L I+
Sbjct: 1   MGYERLG---PSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVS--SIVLAIS 60

Query: 61  AAVSATFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNA-N 120
             ++A    G+R+     +S P L RKP+QAISKAC  TRFP LCV+SL+DFPGSL A +
Sbjct: 61  LILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASS 120

Query: 121 EHDLVHISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSV 180
             DL+H++ N+TL H + ALY S+ +S + M  R RSAYD+C+ELLDDSVDAL+R+L SV
Sbjct: 121 SKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSV 180

Query: 181 APPSSDGTPHRLASSDDIITWLSAALTNQDTCSDGFSEL-TGNVKDQMTDKLHNLSELVS 240
              S        A   D+ TWLSAALTN DTC++GF  +  G VKD MT  L NLSELVS
Sbjct: 181 VSSS--------AKPQDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVS 240

Query: 241 NCLALFSGS-ETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQA 300
           NCLA+FS S + +DFAGVPIQN RRL+   E       FP WM  +ER +L +PV  +QA
Sbjct: 241 NCLAIFSASHDGDDFAGVPIQN-RRLLGVEER---EEKFPRWMRPKEREILEMPVSQIQA 300

Query: 301 DIVVSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGK 360
           DI+VSKDGNGT KTI+EAIKKAP+ S++R IIYV AGRYEE NLKVGRKK NLMFVGDGK
Sbjct: 301 DIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGK 360

Query: 361 GKTVISGAKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVV 420
           GKTVISG KSIFDN+TTFHTASFAATGAG I RD+TFENWAGP +HQAVALR+GADHAV+
Sbjct: 361 GKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVI 420

Query: 421 YRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTIT 480
           YRCN+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVV QNCSIYARK MD QKNTIT
Sbjct: 421 YRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTIT 480

Query: 481 AQNRKDPNQNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHP 540
           AQNRKDPNQNTGISIHA R+LA SDL ++N S  TYLGRPWKL+SR VYM+SYIG HVH 
Sbjct: 481 AQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHT 540

Query: 541 RGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSS 600
           RGWLEWN TFALDTLYYGEY+N GPG  +GQRV+WPGYRVI S  EA++FTVA+FIYGSS
Sbjct: 541 RGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSS 598

Query: 601 WLPSTGVAFLGGLNV 608
           WLPSTGV+FL GL++
Sbjct: 601 WLPSTGVSFLAGLSI 598

[Close]

BLAST of Tan0016494 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 763.5 bits (1970), Expect = 1.3e-220
Identity = 391/609 (64.20%), Postives = 468/609 (76.85%), Query Frame = 0


Query: 1   MGYGRLNETDPGGSSGAVTFQLSDDAVSFPTPSPNRRRRTRLLLLSLLSLILIIAAAVSA 60
           MGY RL      G SG       D A S   P   ++ +T+L+   L +L +++   V  
Sbjct: 1   MGYDRL------GPSGPSNPNQKDPATSL--PELQKKTKTKLI---LFTLAVLVVGVVCF 60

Query: 61  TFLVGLRTASSSPRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEHDLVH 120
               G+R A  S +++P L RKPTQAIS+ CS++ +P LC+++LLDFPGSL A+E++L+H
Sbjct: 61  GIFAGIR-AVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIH 120

Query: 121 ISFNLTLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN TLQ  +KALY SS I+  QM  RVRSAYD+CLELLDDSVDALTR+L SV   S D
Sbjct: 121 ISFNATLQKFSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGD 180

Query: 181 GTPHRLASSDDIITWLSAALTNQDTCSDGFSELT---GNVKDQMTDKLHNLSELVSNCLA 240
                  S  D++TWLS+A+TN DTC+DGF E+    G VKDQ+   + +LSE+VSNCLA
Sbjct: 181 ------ESHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLA 240

Query: 241 LFSGSETNDFAGVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVS 300
           +F+G +  D +GVP+ N R+L+   E    +   P W+ R +R LL  P  A+QADI VS
Sbjct: 241 IFAG-KVKDLSGVPVVNNRKLLGTEE----TEELPNWLKREDRELLGTPTSAIQADITVS 300

Query: 301 KDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVI 360
           KDG+GT KTIAEAIKKAPE+SS+R +IYV AGRYEE+NLKVGRKKTNLMF+GDGKGKTVI
Sbjct: 301 KDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVI 360

Query: 361 SGAKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNV 420
           +G KSI D++TTFHTA+FAATGAG I+RDMTFEN+AGP +HQAVALRVG DHAVVYRCN+
Sbjct: 361 TGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNI 420

Query: 421 IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRK 480
           IGYQD LYVHSNRQFFREC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK TITAQNRK
Sbjct: 421 IGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRK 480

Query: 481 DPNQNTGISIHACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLE 540
           DPNQNTGISIHAC++LAT DL +S  S PTYLGRPWKLYSRVVYM+S +G H+ PRGWLE
Sbjct: 481 DPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLE 540

Query: 541 WNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPST 600
           WN  FALD+LYYGEYMN G G  +GQRV WPGY VITS VEASKFTVAQFI GSSWLPST
Sbjct: 541 WNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPST 586

Query: 601 GVAFLGGLN 607
           GV+F  GL+
Sbjct: 601 GVSFFSGLS 586

[Close]

BLAST of Tan0016494 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 439.1 bits (1128), Expect = 5.7e-123
Identity = 247/591 (41.79%), Postives = 349/591 (59.05%), Query Frame = 0


Query: 35  NRRRRTRLLLLSLLSLILIIAAAVSATFLVGLRTASSSPRSDPNLPRKPTQAISKACSRT 94
           N ++  +L+LLS    +L +AA      + G+   +S       L       +  +CS T
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAA------VAGISAGASKANEKRTLSPSSHAVLRSSCSST 72

Query: 95  RFPTLCVNSLLDFPGSLNANEHDLVHISFNLTLQHLNKALYLSSEISSLQMDL--RVRSA 154
           R+P LC+++++   G    ++ D++  S NLT+  +    +   ++   +  L  R ++A
Sbjct: 73  RYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 132

Query: 155 YDACLELLDDSVDALTRSLQSVAPPSSDGTPHRLASSDDIITWLSAALTNQDTCSDGFS- 214
              CLE +D+++D L  +++ +    +  T    A   D+ T +S+A+TNQ+TC DGFS 
Sbjct: 133 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAG--DLKTLISSAITNQETCLDGFSH 192

Query: 215 -ELTGNVKDQMTDKLHNLSELVSNCLALFSGSETNDFAGVP-----IQNKRRLMD----- 274
            +    V+  +     ++  + SN LA+       D A          N R+L +     
Sbjct: 193 DDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQET 252

Query: 275 ------AGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKA 334
                 AG   + S  +P W+S  +RRL  L    ++AD  V+ DG+GT KT+A A+  A
Sbjct: 253 TVAVDIAGAGELDSEGWPTWLSAGDRRL--LQGSGVKADATVAADGSGTFKTVAAAVAAA 312

Query: 335 PEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTAS 394
           PE S+KR +I++ AG Y E N++V +KK N+MF+GDG+ +T+I+G++++ D  TTFH+A+
Sbjct: 313 PENSNKRYVIHIKAGVYRE-NVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSAT 372

Query: 395 FAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGYQDTLYVHSNRQFFR 454
            AA G   + RD+TF+N AGP +HQAVALRVG+D +  Y C+++ YQDTLYVHSNRQFF 
Sbjct: 373 VAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFV 432

Query: 455 ECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILA 514
           +C I GTVDFIFGNAAVV Q+C I+AR+    QKN +TAQ R DPNQNTGI I  CRI A
Sbjct: 433 KCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGA 492

Query: 515 TSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMN 574
           TSDL S   S PTYLGRPWK YS+ V M S I   + P GW EW  TFAL+TL Y EY N
Sbjct: 493 TSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSN 552

Query: 575 YGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVAFLGGL 606
            G G     RV W G++VIT+  EA K+T  QFI G  WL STG  F  GL
Sbjct: 553 TGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of Tan0016494 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 425.2 bits (1092), Expect = 8.6e-119
Identity = 242/582 (41.58%), Postives = 349/582 (59.97%), Query Frame = 0


Query: 38  RRTRLLLLSLLSLILIIAAAVSATFLVGLRTASSSPRSDPNLP--RKPTQAISKA-CSRT 97
           +  + L+LS  ++ L++ A++     VG+   +++   +  +      + AI K+ CS T
Sbjct: 15  KNNKKLILSSAAIALLLLASI-----VGIAATTTNQNKNQKITTLSSTSHAILKSVCSST 74

Query: 98  RFPTLCVNSLLDFPGSLNANEHDLVHISFNLTLQHLNKALYLSSEISSLQMDLRVR--SA 157
            +P LC +++    G    ++ +++  S NLT + +    +   ++ + +  L  R  +A
Sbjct: 75  LYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTA 134

Query: 158 YDACLELLDDSVDALTRSLQSV--APPSSDGTPHRLASSDDIITWLSAALTNQDTCSDGF 217
              CLE +D+++D L  +++ +   P       H    +DD+ T +S+A+TNQ TC DGF
Sbjct: 135 LHDCLETIDETLDELHVAVEDLHQYPKQKSLRKH----ADDLKTLISSAITNQGTCLDGF 194

Query: 218 S--ELTGNVKDQMTDKLHNLSELVSNCLALFSGSETNDFAGVPIQ---------NKRRLM 277
           S  +    V+  +     ++  + SN LA+       D A   ++         N R+L 
Sbjct: 195 SYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLK 254

Query: 278 DAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEYSS 337
           +   ++ S G +P W+S  +RRL  L    ++AD  V+ DG+G   T+A A+  APE S+
Sbjct: 255 EVTGDLDSDG-WPKWLSVGDRRL--LQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSN 314

Query: 338 KRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTASFAATG 397
           KR +I++ AG Y E N++V +KKTN+MF+GDG+GKT+I+G++++ D  TTFH+A+ AA G
Sbjct: 315 KRFVIHIKAGVYRE-NVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVG 374

Query: 398 AGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIY 457
              + RD+TF+N AGP +HQAVALRVG+D +  Y+C++  YQDTLYVHSNRQFF +C I 
Sbjct: 375 ERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHIT 434

Query: 458 GTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILATSDLS 517
           GTVDFIFGNAA V Q+C I AR+    QKN +TAQ R DPNQNTGI I  CRI  TSDL 
Sbjct: 435 GTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLL 494

Query: 518 SSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMNYGPGG 577
           +   + PTYLGRPWK YSR V M S I   + P GW EW+ +FALDTL Y EY+N G G 
Sbjct: 495 AVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGA 554

Query: 578 AVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVAF 602
               RV W GY+VITS  EA  FT  QFI G  WL STG  F
Sbjct: 555 GTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

[Close]

BLAST of Tan0016494 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 417.9 bits (1073), Expect = 1.4e-116
Identity = 223/477 (46.75%), Postives = 300/477 (62.89%), Query Frame = 0


Query: 130 LNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQ-SVAPPSSDGTPHRLAS 189
           + KA+ + S+        RV +A   C++LLD + + L+  +  S +P   D +   + S
Sbjct: 58  ITKAVAIVSKFDKKAGKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGS 117

Query: 190 SDDIITWLSAALTNQDTCSDGFSELTGNVKDQMTDKLHNLSELVSNCLALFSGSETNDFA 249
             D+ TW+SAAL+NQDTC DGF    G +K  +   L  +   V N L +     +    
Sbjct: 118 --DLRTWISAALSNQDTCLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKP-- 177

Query: 250 GVPIQNKRRLMDAGENVVSSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIA 309
             P   K + M    +  S   FP W+   +R+LL        AD VV+ DG G   TI+
Sbjct: 178 -KPKPIKAQTMTKAHSGFS--KFPSWVKPGDRKLLQTD-NITVADAVVAADGTGNFTTIS 237

Query: 310 EAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVT 369
           +A+  AP+YS+KR +I+V  G Y E N+++ +KK N+M VGDG   TVI+G +S  D  T
Sbjct: 238 DAVLAAPDYSTKRYVIHVKRGVYVE-NVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWT 297

Query: 370 TFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNVIGYQDTLYVHS 429
           TF +A+FA +G G I RD+TF+N AGP +HQAVA+R   D  V YRC + GYQDTLY HS
Sbjct: 298 TFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHS 357

Query: 430 NRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIH 489
            RQFFREC I GTVDFIFG+A  VFQ+C I A++ +  QKN+ITAQ RKDPN+ TG +I 
Sbjct: 358 MRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQ 417

Query: 490 ACRILATSDLSSSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNATFALDTLY 549
              I A +DL  + ++  TYLGRPWKLYSR V+M +Y+   ++P GWLEWN  FALDTLY
Sbjct: 418 FSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLY 477

Query: 550 YGEYMNYGPGGAVGQRVTWPGYRVITSPVEASKFTVAQFIYGSSWLPSTGVAFLGGL 606
           YGEYMN GPG ++ +RV WPGY V+ +  EA+ FTV+Q I G+ WLPSTG+ F+ GL
Sbjct: 478 YGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:

BLAST of Tan0016494 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M3B0	4.9e-228	66.18	Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3...	[more]
Q9FK05	1.9e-219	64.20	Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3...	[more]
P83948	4.7e-122	42.66	Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1	[more]
O49006	8.1e-122	41.79	Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME...	[more]
O04886	1.8e-121	42.83	Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1	[more]

BLAST of Tan0016494 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881267.1	0.0e+00	91.10	probable pectinesterase/pectinesterase inhibitor 34 [Benincasa hispida]	[more]
XP_022922767.1	0.0e+00	90.12	probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita moschata]	[more]
XP_022984620.1	0.0e+00	90.12	probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita maxima]	[more]
XP_023553011.1	0.0e+00	89.95	probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita pepo subsp. pepo]	[more]
KAA0050503.1	3.0e-307	88.89	pectin methylesterase family protein [Cucumis melo var. makuwa]	[more]

BLAST of Tan0016494 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E487	0.0e+00	90.12	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1	[more]
A0A6J1J930	0.0e+00	90.12	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111482853 PE=3 SV=1	[more]
A0A5A7U3N7	1.4e-307	88.89	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00145...	[more]
A0A0A0L6S0	2.5e-307	88.73	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G239880 PE=3 SV=1	[more]
A0A6J1G0F6	7.5e-280	82.59	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449561 PE=3 SV=1	[more]

BLAST of Tan0016494 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49220.1	3.5e-229	66.18	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G53370.1	1.3e-220	64.20	pectin methylesterase PCR fragment F	[more]
AT3G14310.1	5.7e-123	41.79	pectin methylesterase 3	[more]
AT1G53830.1	8.6e-119	41.58	pectin methylesterase 2	[more]
AT3G43270.1	1.4e-116	46.75	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 82..238 e-value: 1.0E-44 score: 164.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 62..239 e-value: 1.2E-38 score: 130.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 86..238 e-value: 3.9E-32 score: 111.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 84..241 e-value: 7.0E-37 score: 128.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 85..240
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 293..590 e-value: 2.5E-141 score: 469.7
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 287..605 e-value: 5.8E-117 score: 392.6
None	No IPR available	PANTHER	PTHR31707:SF222	PECTINESTERASE	coord: 33..605
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 33..605
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 90..240 e-value: 9.58087E-47 score: 159.526
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 438..447
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 289..603

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0016494.1	Tan0016494.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity