Tan0016302 (gene) Snake gourd v1

Overview
NameTan0016302
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionGDSL esterase/lipase
LocationLG04: 1163125 .. 1166131 (+)
RNA-Seq ExpressionTan0016302
SyntenyTan0016302
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTAACTGCTTCTAATCCTAAATCTAAACAGATCCACTTTTCCCACTCCCTCATCTCCATACATATATACACTTATCTTTCATTCACATTTTGAATTCTCTCTCTTTTTCTCAATGGAGTGCCATTTCTTAATGATTGCTTTCACCACTTTCTTGGCACTGTCACTGATTCCAAGCTCTGTCCTGGCCCGAGAGCCCTGCAATTTCCCGGCTGTTTTCAACTTCGGCGATTCCAATTCCGACACCGGCGGCCTCTCCGCCCTCTATGGCCAGGCCCCGCCGCCCAATGGCATGTCCTACTTCCGTGGCCCCGCCGGCCGCTACTGCGACGGCCGTCTCATCGTCGATTTCATAGGTATTTTGAAGTTTACTGAGATCTTTACTCTTGGGATTTCTTCACTTTTGATGGGTTTCGTTGATTGATTTTAAAATTTTAGCCAAAAGCCTCGGATTGCCTTATCTAAGCGCGTATCTTGATGCGTTGGGAGCCAATTTCACTCACGGAGCCAATTTCGCCACTGCTGGTTCAACTGTTAGGGTACAGAACAGAACTATCCAGCAGAGTGGATTCAGCCCCATTTCTCTCAATGTTCAGTTCAATGAATTCTATGACTTCCGTCGCAGATCCCAAACCGTTCGAAATCGATGTGAGTGACAGTGAGAGTTTTTGCCAAATTTGAAATCGGTGAAGTTAATTATTTTCTTTTATGTTGTGAACTAACTTTGATTTTGATTTTTCAGTCGGTGGTGTTTTTGAACAATTGTTGCCGGAGGAAGATACTTTCAGTCGGGCGCTGTACACATTTGACATCGGTCAGAACGATTTGACTAGTGGTTACTTCGCCAATATGACCGTCGATCAAGTTAGAGAGTACATCCCTGAAGTTCTCGACCAGTTCTCCAATATTGTCAAGGTCAGTCTTTTACACTTTTTTTTTTTTTTTTACTCCTCTTCAATACCCATATTAAATTTTTTCTCTAATTTTAAAAATATTAAAGTTGTTGTGTAAGAACAGTTTGCAACCTAACCATACTCACCTTGGCAAATGGACAAAGATTTTAAATGGTGTAAATAGAGTCGTTCAAGTGGGATATTGGAAAATTTAATATGAGCAAAAATACATATCAAATGTAATAATGTGGTTAACACATGAGCTATAAAAGCCAACAAGATAGCTCGACTGTCATCAAGTATGACTTATGACCAAGAGGTCATAGGTTCGAATCTCTCACTCCCAATTGTTGATGTACTCAAAAAAAAAAACACATAAGCTATAAAAAGAAAGTACACTAATAATAAAAAATAGTTAAGAGTCGTATGGAGATCATATTATAATAGTTTATAAAAAAGGAAAAAAAAAACTGATAACAAAGTCTAAATTTAAAGCTAGGAAGTTTTGTTTTGAAAATAGTAGGTGAATGTGAGTATGAAGGAAATGGATTTGAGGGGTAAAAAGGGAAAAACACGCACACACAACCGACACCAAACACTAATTATGCATACATATTATTAATTATATATGTAAAAATGTTATAAACGTAATCTAACAATTTTATGGGGCGTATGAATGTAATGCAGTGGGTGCACGGTCAAGGGGGGAGATTCTTCTGGATCCATAACACAGGCCCAGTGGGCTGCCTTCCATATGTATTGGACCGAAAGCCCGTTTCAGCCTCAGACTATGACCAATATGGATGTGCCACTCCTTTCAATGACTTGGCCCAATCGTTCAACCGCGGCCTTAAGCAAACCGTCGTTGAGCTTCGCGCCGCTTTGCCCGATGCCGCCATCACCTACGTCGATGTTTACTCCCTCAAATACGCTCTCATTTCCCAACATAAAAAACATGGTAATACTATTTTTCTTCCTCATTTATATTTATATCGCTTTTTTTTTTTTTTGTTACAACATAGAGTTCATGAATCTTTAAAGAATAAATATAATTAAATTTATCCTACTTCATTGGTCTAAGTTTAGTTTTGATTTAATATTAGTATGTTACGAATGGGGATAATATTTCATACCATTAAAGCAAAAAAATACATTCGAATGCAAAAACTACTGATGAACTTTGGAGTTTGTTACAATCTCTTACTTTGTTAAGTGTTACCATTAACCCTTACTTTTTCTTCTTCTTCTTTTTCCCTTAAAAAATGGTTGATTGTAACACTTAATAAAGTTTATTAAGTGTGATTGTAATAAACCACATTGTTTTAAATGATACACTTTTACTTCTGTCTTTTCATTTGGTTTTAGATTAGTGCTCACATTTGATACTTTGTGTTAATTTTTTATTGCTTAATTTAAAAGAATTAAATTAGTCCATTAGAATAGTTCTCACTATTTATCAAAAAAAGAAAAAAAAAAACAAATAGTTATCACTAAGTTTTCGTCCCTATCAAAATAGTAATCTCGTGTTTAAACTCTTGTGGTACCAAATTTTATCTAATTAAAATATTGGTTTTCACATGTTAGATTTCTTTTAGATTTAGTGGCAACAATGATTTAACGATAAAACGATGAAATTGAAATGTAGGGTTCGAGTTTCCGCTGAGAACATGTTGTGGACATGGTGGAAAATACAACTTCAATGTGAATCTTGGATGTGGAGGAAGAAAATGGATCAATGGGACGGATGTTTTGATTGGAAAAGCTTGTAAAAAGCCAAAAGTGTATGTGAATTGGGATGGAGTTCATCTCACTCAAGCAGCTAACAAATGGATCTTCAATCAAATTAAAACTGGTGCTTATTCAGAACCACCTATCCCTTTAAAAATGGCATGTCACAGAGCCTAATCTAATCCAATCCAATCCCATTATTGTATCACAATTACTGTTGCTTCTGCTTCTTTCTTTAGTTTGCTCTTTTGGCCCAAGTGTTTATCATACAAGTGATTTTAGGCACATAGCTAGGTACAAATGTCACTATCCCTTTTCTCTTCTTTCTCTAGTAACATCATTTGGATTCTGGTGTTGGTTTAGTTTTATCTTAATAATATTAGCTACA

mRNA sequence

CTTAACTGCTTCTAATCCTAAATCTAAACAGATCCACTTTTCCCACTCCCTCATCTCCATACATATATACACTTATCTTTCATTCACATTTTGAATTCTCTCTCTTTTTCTCAATGGAGTGCCATTTCTTAATGATTGCTTTCACCACTTTCTTGGCACTGTCACTGATTCCAAGCTCTGTCCTGGCCCGAGAGCCCTGCAATTTCCCGGCTGTTTTCAACTTCGGCGATTCCAATTCCGACACCGGCGGCCTCTCCGCCCTCTATGGCCAGGCCCCGCCGCCCAATGGCATGTCCTACTTCCGTGGCCCCGCCGGCCGCTACTGCGACGGCCGTCTCATCGTCGATTTCATAGCCAAAAGCCTCGGATTGCCTTATCTAAGCGCGTATCTTGATGCGTTGGGAGCCAATTTCACTCACGGAGCCAATTTCGCCACTGCTGGTTCAACTGTTAGGGTACAGAACAGAACTATCCAGCAGAGTGGATTCAGCCCCATTTCTCTCAATGTTCAGTTCAATGAATTCTATGACTTCCGTCGCAGATCCCAAACCGTTCGAAATCGATTCGGTGGTGTTTTTGAACAATTGTTGCCGGAGGAAGATACTTTCAGTCGGGCGCTGTACACATTTGACATCGGTCAGAACGATTTGACTAGTGGTTACTTCGCCAATATGACCGTCGATCAAGTTAGAGAGTACATCCCTGAAGTTCTCGACCAGTTCTCCAATATTGTCAAGTGGGTGCACGGTCAAGGGGGGAGATTCTTCTGGATCCATAACACAGGCCCAGTGGGCTGCCTTCCATATGTATTGGACCGAAAGCCCGTTTCAGCCTCAGACTATGACCAATATGGATGTGCCACTCCTTTCAATGACTTGGCCCAATCGTTCAACCGCGGCCTTAAGCAAACCGTCGTTGAGCTTCGCGCCGCTTTGCCCGATGCCGCCATCACCTACGTCGATGTTTACTCCCTCAAATACGCTCTCATTTCCCAACATAAAAAACATGGGTTCGAGTTTCCGCTGAGAACATGTTGTGGACATGGTGGAAAATACAACTTCAATGTGAATCTTGGATGTGGAGGAAGAAAATGGATCAATGGGACGGATGTTTTGATTGGAAAAGCTTGTAAAAAGCCAAAAGTGTATGTGAATTGGGATGGAGTTCATCTCACTCAAGCAGCTAACAAATGGATCTTCAATCAAATTAAAACTGGTGCTTATTCAGAACCACCTATCCCTTTAAAAATGGCATGTCACAGAGCCTAATCTAATCCAATCCAATCCCATTATTGTATCACAATTACTGTTGCTTCTGCTTCTTTCTTTAGTTTGCTCTTTTGGCCCAAGTGTTTATCATACAAGTGATTTTAGGCACATAGCTAGGTACAAATGTCACTATCCCTTTTCTCTTCTTTCTCTAGTAACATCATTTGGATTCTGGTGTTGGTTTAGTTTTATCTTAATAATATTAGCTACA

Coding sequence (CDS)

ATGGAGTGCCATTTCTTAATGATTGCTTTCACCACTTTCTTGGCACTGTCACTGATTCCAAGCTCTGTCCTGGCCCGAGAGCCCTGCAATTTCCCGGCTGTTTTCAACTTCGGCGATTCCAATTCCGACACCGGCGGCCTCTCCGCCCTCTATGGCCAGGCCCCGCCGCCCAATGGCATGTCCTACTTCCGTGGCCCCGCCGGCCGCTACTGCGACGGCCGTCTCATCGTCGATTTCATAGCCAAAAGCCTCGGATTGCCTTATCTAAGCGCGTATCTTGATGCGTTGGGAGCCAATTTCACTCACGGAGCCAATTTCGCCACTGCTGGTTCAACTGTTAGGGTACAGAACAGAACTATCCAGCAGAGTGGATTCAGCCCCATTTCTCTCAATGTTCAGTTCAATGAATTCTATGACTTCCGTCGCAGATCCCAAACCGTTCGAAATCGATTCGGTGGTGTTTTTGAACAATTGTTGCCGGAGGAAGATACTTTCAGTCGGGCGCTGTACACATTTGACATCGGTCAGAACGATTTGACTAGTGGTTACTTCGCCAATATGACCGTCGATCAAGTTAGAGAGTACATCCCTGAAGTTCTCGACCAGTTCTCCAATATTGTCAAGTGGGTGCACGGTCAAGGGGGGAGATTCTTCTGGATCCATAACACAGGCCCAGTGGGCTGCCTTCCATATGTATTGGACCGAAAGCCCGTTTCAGCCTCAGACTATGACCAATATGGATGTGCCACTCCTTTCAATGACTTGGCCCAATCGTTCAACCGCGGCCTTAAGCAAACCGTCGTTGAGCTTCGCGCCGCTTTGCCCGATGCCGCCATCACCTACGTCGATGTTTACTCCCTCAAATACGCTCTCATTTCCCAACATAAAAAACATGGGTTCGAGTTTCCGCTGAGAACATGTTGTGGACATGGTGGAAAATACAACTTCAATGTGAATCTTGGATGTGGAGGAAGAAAATGGATCAATGGGACGGATGTTTTGATTGGAAAAGCTTGTAAAAAGCCAAAAGTGTATGTGAATTGGGATGGAGTTCATCTCACTCAAGCAGCTAACAAATGGATCTTCAATCAAATTAAAACTGGTGCTTATTCAGAACCACCTATCCCTTTAAAAATGGCATGTCACAGAGCCTAA

Protein sequence

MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGMSYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTIQQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLTSGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSASDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGFEFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKWIFNQIKTGAYSEPPIPLKMACHRA
Homology
BLAST of Tan0016302 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 3.9e-138
Identity = 231/374 (61.76%), Postives = 289/374 (77.27%), Query Frame = 0

Query: 10  FTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGMSYFRGPAGR 69
           F   L  SL PS+      C+FPA+FNFGDSNSDTGGLSA +GQA PP+G S+F  PAGR
Sbjct: 9   FALSLLSSLSPST--HAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGR 68

Query: 70  YCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTIQQSGFSPIS 129
           YCDGRL++DFIA+SLGLPYLSA+LD++G+NF+HGANFATAGS +R  N T++QSGFSP S
Sbjct: 69  YCDGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFS 128

Query: 130 LNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLTSGYFANMTV 189
           L+VQF +FY+F  RSQTVR+R GGV++ +LPE D+FS+ALYTFDIGQNDLT+GYFAN TV
Sbjct: 129 LDVQFVQFYNFHNRSQTVRSR-GGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFANKTV 188

Query: 190 DQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSASDYDQYGCA 249
           +QV   +PE++ QF N +K ++GQGGR+FWIHNTGP+GCL YV++R P  ASD+D +GC 
Sbjct: 189 EQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCV 248

Query: 250 TPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGFEFPLRTCCG 309
           +P N LAQ FN  LKQ V+ELR++L +AAITYVDVYSLK+ L    + HGF+  L +CCG
Sbjct: 249 SPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCG 308

Query: 310 HGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKWIFNQIKTGA 369
           HGGKYN+N  +GCG +K + G +V IGK C +P   V WDGVH TQAANK+IF++I  G 
Sbjct: 309 HGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG- 368

Query: 370 YSEPPIPLKMACHR 384
                  L  AC R
Sbjct: 369 -------LSKACKR 371

BLAST of Tan0016302 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 5.3e-135
Identity = 223/383 (58.22%), Postives = 288/383 (75.20%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           ME + L++      +  + P +      CNFPA+FNFGDSNSDTGGLSA +GQAP PNG 
Sbjct: 1   METNLLLVKCVLLASCLIHPRA--CSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQ 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           ++F  P+GR+ DGRLI+DFIA+ LGLPYL+A+LD++G+NF+HGANFATAGSTVR  N TI
Sbjct: 61  TFFHSPSGRFSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATI 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
            QSG SPISL+VQ  +F DF  RSQ +RNR GGVF++LLP+++ FS+ALYTFDIGQNDLT
Sbjct: 121 AQSGVSPISLDVQLVQFSDFITRSQLIRNR-GGVFKKLLPKKEYFSQALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           +G   NMT DQ++ YIP+V DQ SN+++ V+ +GGR FWIHNT P+GCLPYVLDR PV A
Sbjct: 181 AGLKLNMTSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPA 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           S  D +GCA P N++A+ +N  LK+ V+ELR  L +AA TYVD+YS+K  LI+Q KK GF
Sbjct: 241 SQIDNHGCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
            +PL  CCGHGGKYNFN  + CG +  I G ++++ K+C      V+WDG+H T+  N W
Sbjct: 301 RYPLVACCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHR 384
           IF QI  GA+S+PP+P+K AC R
Sbjct: 361 IFQQINDGAFSDPPLPVKSACTR 380

BLAST of Tan0016302 vs. ExPASy Swiss-Prot
Match: B4FZ87 (GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 4.9e-133
Identity = 219/378 (57.94%), Postives = 291/378 (76.98%), Query Frame = 0

Query: 6   LMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGMSYFRG 65
           +++  +  LAL+L PS   A   C+FPAVFNFGDSNSDTGGLS+L+G APPPNG ++F  
Sbjct: 15  VLLLLSLALALALRPSDAGAGGDCHFPAVFNFGDSNSDTGGLSSLFGAAPPPNGRTFFGM 74

Query: 66  PAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTIQQSGF 125
           PAGRYCDGRL++DFIA+SLGL +LSAYL+++G+NFT GANFATAGS++R QN ++  SGF
Sbjct: 75  PAGRYCDGRLVIDFIAESLGLTHLSAYLNSIGSNFTQGANFATAGSSIRRQNTSLFLSGF 134

Query: 126 SPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLTSGYFA 185
           SPISL+VQF EF  F  RSQ V N  GG++ ++LP  + FS+ALYTFDIGQND+TS YF 
Sbjct: 135 SPISLDVQFWEFEQFINRSQLVYNNKGGIYREILPRAEYFSQALYTFDIGQNDITSSYFV 194

Query: 186 NMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSASDYDQ 245
           N T ++V   IP+++++ ++I++ V+ +GGR+FWIHNTGP+GCLPY L  +P  A   D 
Sbjct: 195 NNTTEEVEAIIPDLMERLTSIIQSVYSRGGRYFWIHNTGPLGCLPYALLHRPDLAIPADG 254

Query: 246 YGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGFEFPLR 305
            GC+  +N +AQ FN  LK+TV  LR   PDAA TYVDVY+ KY LISQ  K GF+ PL 
Sbjct: 255 TGCSVTYNKVAQLFNLRLKETVASLRKTHPDAAFTYVDVYTAKYKLISQANKLGFDDPLL 314

Query: 306 TCCGH-GGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKWIFNQ 365
           TCCG+ GG+YN ++++GCGG+K +NGT V++GK+C+ P   V+WDGVH T+AANK++F+Q
Sbjct: 315 TCCGYGGGRYNLDLSVGCGGKKQVNGTSVVVGKSCENPSKRVSWDGVHFTEAANKFVFDQ 374

Query: 366 IKTGAYSEPPIPLKMACH 383
           I  GA S+PP+ L+ ACH
Sbjct: 375 IVAGALSDPPVALRQACH 392

BLAST of Tan0016302 vs. ExPASy Swiss-Prot
Match: Q7Y1X1 (Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 9.6e-113
Identity = 196/365 (53.70%), Postives = 260/365 (71.23%), Query Frame = 0

Query: 21  SSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGMSYFRGPAGRYCDGRLIVDFI 80
           S   A E C+FPA+FNFGDSNSDTGG +A +    PP G ++F    GRY DGRLI+DFI
Sbjct: 22  SLAYASETCDFPAIFNFGDSNSDTGGKAAAFYPLNPPYGETFFHRSTGRYSDGRLIIDFI 81

Query: 81  AKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI-QQSGFSPISLNVQFNEFYD 140
           A+S  LPYLS YL +LG+NF HGA+FATAGST+++    I    GFSP  L+VQ+++F  
Sbjct: 82  AESFNLPYLSPYLSSLGSNFKHGADFATAGSTIKLPTTIIPAHGGFSPFYLDVQYSQFRQ 141

Query: 141 FRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLTSGYFANMTVDQVREYIPEV 200
           F  RSQ +R   GG+F +L+PEE  F +ALYTFDIGQNDLT G F N+TV++V   +P++
Sbjct: 142 FIPRSQFIRET-GGIFAELVPEEYYFEKALYTFDIGQNDLTEG-FLNLTVEEVNATVPDL 201

Query: 201 LDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSASDYDQYGCATPFNDLAQSF 260
           ++ FS  VK ++  G R FWIHNTGP+GCL ++L   P   ++ D  GCA  +N++AQ F
Sbjct: 202 VNSFSANVKKIYDLGARTFWIHNTGPIGCLSFILTYFP--WAEKDSAGCAKAYNEVAQHF 261

Query: 261 NRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGFEFPLRTCCGHGGKYNFNVN 320
           N  LK+ V +LR  LP A   +VD+YS+KY+L S+ +KHGFEFPL TCCG+GGKYNF+V 
Sbjct: 262 NHKLKEIVAQLRKDLPLATFVHVDIYSVKYSLFSEPEKHGFEFPLITCCGYGGKYNFSVT 321

Query: 321 LGCGGRKWI-NGTDVLIGKACKKPKVYVNWDGVHLTQAANKWIFNQIKTGAYSEPPIPLK 380
             CG      +GT +++G +C  P V VNWDG H T+AAN++ F+QI TGA+S+PP+PL 
Sbjct: 322 APCGDTVTADDGTKIVVG-SCACPSVRVNWDGAHYTEAANEYFFDQISTGAFSDPPVPLN 381

Query: 381 MACHR 384
           MACH+
Sbjct: 382 MACHK 381

BLAST of Tan0016302 vs. ExPASy Swiss-Prot
Match: Q9LY84 (GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 5.0e-101
Identity = 183/384 (47.66%), Postives = 256/384 (66.67%), Query Frame = 0

Query: 6   LMIAFTTF----LALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGMS 65
           LM++ T F    L L  + +SV  +  C FPA++NFGDSNSDTGG+SA +     P G  
Sbjct: 10  LMVSSTVFSWLLLCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQG 69

Query: 66  YFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTIQ 125
           +F  P GR  DGRL +DFIA+ LGLPYLSAYL++LG+NF HGANFAT GST+R QN TI 
Sbjct: 70  FFHRPTGRDSDGRLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQNETIF 129

Query: 126 QSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFE-QLLPEEDTFSRALYTFDIGQNDLT 185
           Q G SP SL++Q  +F  F+ RS  +  +    ++ + LP ++ F++ALYTFDIGQNDL+
Sbjct: 130 QYGISPFSLDMQIAQFDQFKARSALLFTQIKSRYDREKLPRQEEFAKALYTFDIGQNDLS 189

Query: 186 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 245
            G F  M+VDQ++  IP++++  ++ V+ ++ QGGR FW+HNTGP GCLP  +      A
Sbjct: 190 VG-FRTMSVDQLKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPA 249

Query: 246 SDY-DQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHG 305
             Y D+ GC    N++A  FNR LK+TV+ LR  L  AAITYVDVY+ KY ++S  KK G
Sbjct: 250 PGYLDKSGCVKAQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLG 309

Query: 306 FEFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANK 365
           F  PL+ CCG+  KY+   ++ CG +  +N T++  G +C  P + V+WDGVH T+AANK
Sbjct: 310 FANPLKVCCGYHEKYD---HIWCGNKGKVNNTEI-YGGSCPNPVMAVSWDGVHYTEAANK 369

Query: 366 WIFNQIKTGAYSEPPIPLKMACHR 384
            + ++   G  ++PP+P+  AC+R
Sbjct: 370 HVADRTLNGLLTDPPVPITRACYR 388

BLAST of Tan0016302 vs. NCBI nr
Match: XP_038891379.1 (GDSL esterase/lipase At3g26430 [Benincasa hispida])

HSP 1 Score: 720.3 bits (1858), Expect = 8.7e-204
Identity = 337/386 (87.31%), Postives = 362/386 (93.78%), Query Frame = 0

Query: 1   MECHFLMIAFTTF--LALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPN 60
           MECH L+IAFTTF  LALS+IP SVLAREPCNFPAVFNFGDSNSDTGGLSA++GQAPPPN
Sbjct: 18  MECHSLIIAFTTFIALALSVIPCSVLAREPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPN 77

Query: 61  GMSYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNR 120
           G SYF GPAGRY DGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGST+R QN 
Sbjct: 78  GRSYFPGPAGRYSDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTIRPQNT 137

Query: 121 TIQQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQND 180
           T+QQSGFSPISLNVQ+NEFYDFRRRSQT+RNR GG+FEQLLP+E++FSRALYTFDIGQND
Sbjct: 138 TLQQSGFSPISLNVQYNEFYDFRRRSQTLRNRLGGIFEQLLPKEESFSRALYTFDIGQND 197

Query: 181 LTSGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPV 240
           LTSGYF NMTVD+V+ YIPEVL QFS IVKWVH QGGRFFWIHNTGPVGCLPYVLDRKPV
Sbjct: 198 LTSGYFTNMTVDEVKLYIPEVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRKPV 257

Query: 241 SASDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKH 300
           +ASDYDQYGCATPFNDLAQSFNRGLKQ V+ELR ALPDAAITYVD+YSLKYAL++QHKK+
Sbjct: 258 AASDYDQYGCATPFNDLAQSFNRGLKQAVLELRKALPDAAITYVDIYSLKYALVAQHKKY 317

Query: 301 GFEFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAAN 360
           GFEFPLRTCCGHGGKYNFN+ LGCGG+K ING DVLIGK+CK P+VYVNWDGVH TQAAN
Sbjct: 318 GFEFPLRTCCGHGGKYNFNLKLGCGGKKKINGKDVLIGKSCKNPEVYVNWDGVHYTQAAN 377

Query: 361 KWIFNQIKTGAYSEPPIPLKMACHRA 385
           KWIFNQIK G YS+PPIPLKMACHRA
Sbjct: 378 KWIFNQIKDGFYSDPPIPLKMACHRA 403

BLAST of Tan0016302 vs. NCBI nr
Match: XP_023541593.1 (alpha-L-fucosidase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 700.7 bits (1807), Expect = 7.2e-198
Identity = 326/384 (84.90%), Postives = 354/384 (92.19%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           MECH LMIA     AL L+P SVLAR+PCNFPAVFNFGDSNSDTGGLSA++GQAPPPNGM
Sbjct: 1   MECHSLMIA---VFALLLVPHSVLARKPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGM 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           SYF GPAGRYCDGRL++DFIA+SLGLPYLSAYL+ALGANF+HGANFATAGSTVR QNRT+
Sbjct: 61  SYFHGPAGRYCDGRLVIDFIAQSLGLPYLSAYLNALGANFSHGANFATAGSTVRPQNRTL 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
           QQSGFSPISL+VQ+NEFYDFRRRSQTVRNR GG F+ LLP+E +F RALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLDVQYNEFYDFRRRSQTVRNRLGGFFKPLLPKETSFGRALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           SGYFANMTV QVR+YIP+VL QFS IVKWVH QGGRFFWIHNTGPVGCLPYVLDR PVS 
Sbjct: 181 SGYFANMTVKQVRDYIPQVLGQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRMPVSP 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           SDYDQYGCA PFNDLAQ+FNRGLK  VV+LRAALPDAA+TYVD+YSLKY L+SQHKKHGF
Sbjct: 241 SDYDQYGCAIPFNDLAQTFNRGLKDAVVKLRAALPDAALTYVDIYSLKYDLVSQHKKHGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
           EFPLRTCCGHGGKYNFNV LGC G+K +NG DVLIGKAC+KP+VYVNWDGVH TQAANKW
Sbjct: 301 EFPLRTCCGHGGKYNFNVKLGCAGKKRMNGKDVLIGKACEKPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHRA 385
           IFNQIKTGAYS+PPIPL+MACHRA
Sbjct: 361 IFNQIKTGAYSDPPIPLEMACHRA 381

BLAST of Tan0016302 vs. NCBI nr
Match: XP_022966842.1 (GDSL esterase/lipase At3g26430 [Cucurbita maxima])

HSP 1 Score: 700.3 bits (1806), Expect = 9.4e-198
Identity = 325/384 (84.64%), Postives = 355/384 (92.45%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           MECH LMIA     AL L+P SVLAR+PCNFPAVFNFGDSNSDTGGLSA++GQAPPPNGM
Sbjct: 1   MECHSLMIA---VFALLLVPHSVLARKPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGM 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           SYF GPAGRYCDGRL++DFIA+SLGLPYLSAYL+ALGANF+HGANFATAGSTVR QNRT+
Sbjct: 61  SYFHGPAGRYCDGRLVIDFIAQSLGLPYLSAYLNALGANFSHGANFATAGSTVRPQNRTL 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
           QQSGFSPISL+VQ+NEFYDFRRRSQTVRNR GG F+ LLP+E +F +ALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLDVQYNEFYDFRRRSQTVRNRLGGFFKPLLPKETSFGQALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           SGYFANMTV+QVR+YIP+VLDQFS IVKWVH QGGRFFWIHNTGPVGCLPYVLDR PVS 
Sbjct: 181 SGYFANMTVEQVRDYIPQVLDQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRMPVSP 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           SDYDQYGCA PFNDLAQ+FNRGLK  VVELRAALPDAA+TYVD+YSLKY L+SQHKK+GF
Sbjct: 241 SDYDQYGCAIPFNDLAQTFNRGLKDAVVELRAALPDAALTYVDIYSLKYDLVSQHKKYGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
           EFPLRTCCGHGGKYNFNV LGC G+K +NG DVLIGKAC+KP+VYVNWDGVH TQAANKW
Sbjct: 301 EFPLRTCCGHGGKYNFNVKLGCAGKKRMNGKDVLIGKACEKPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHRA 385
           IFN IKTGAYS+PPIPL+MACHRA
Sbjct: 361 IFNHIKTGAYSDPPIPLEMACHRA 381

BLAST of Tan0016302 vs. NCBI nr
Match: XP_008446147.1 (PREDICTED: GDSL esterase/lipase At3g26430 [Cucumis melo] >KAA0034264.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK15657.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 696.8 bits (1797), Expect = 1.0e-196
Identity = 322/383 (84.07%), Postives = 355/383 (92.69%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           ME H L+IAFTTFLALSLIP   LA+ PCNFPAVFNFGDSNSDTGGLSA++GQAPPPNG 
Sbjct: 1   MESHSLIIAFTTFLALSLIPCPSLAKHPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           SYF GPAGRY DGRLIVDFIA+SLGLPYLSAYLDALGANF+HGANFATAGST+R+QNRT+
Sbjct: 61  SYFPGPAGRYSDGRLIVDFIAESLGLPYLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
           QQSGFSPISLNVQ+NEFYDFRRRSQT+RN  GG+F+QLLP+E++FSRALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           SGYFANM++ QV+ YIP+VL QFS IVKWVH QGGRFFWIHNTGPVGCLPYVLDR+PV A
Sbjct: 181 SGYFANMSLHQVKLYIPDVLQQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREPVPA 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           SDYDQYGCATPFNDLAQ FNRGLKQ V+ELR ALPD+AITYVDVYSLKYAL+SQHKK+GF
Sbjct: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVMELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
           E+PLRTCCGHGGKYNFNV LGCGG+K ING +VLIGK+CK P+VYVNWDGVH TQAANKW
Sbjct: 301 EYPLRTCCGHGGKYNFNVKLGCGGKKTINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHR 384
           IFNQIK G+YS+PPIPL  ACH+
Sbjct: 361 IFNQIKDGSYSDPPIPLNKACHK 383

BLAST of Tan0016302 vs. NCBI nr
Match: XP_004135278.1 (GDSL esterase/lipase At3g26430 [Cucumis sativus] >KGN51744.1 hypothetical protein Csa_009271 [Cucumis sativus])

HSP 1 Score: 692.6 bits (1786), Expect = 2.0e-195
Identity = 320/383 (83.55%), Postives = 354/383 (92.43%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           ME H L+IAFTTFLALSL P   LA++PCNFPAVFNFGDSNSDTGGLSA++GQAPPPNG 
Sbjct: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           SYF GPAGRY DGRLIVDFIA+S GLP+LSAYLDALGANF+HGANFATAGST+R+QNRT+
Sbjct: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
           QQSGFSPISLNVQ+NEFYDFRRRSQT+RN  GG+F+QLLP+E++FSRALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           SGYFANMT+ QV+ Y+P+VL QFS IVKWVH QGGRFFWIHNTGPVGCLPYVLDR+ V A
Sbjct: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           SDYDQYGCATPFNDLAQ FNRGLKQ VVELR ALPD+AITYVDVYSLKYAL+SQHKK+GF
Sbjct: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
           E+PLRTCCGHGGKYNFNVNLGCGG+K ING +VLIGK+CK P+VYVNWDGVH TQAANKW
Sbjct: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHR 384
           IFNQIK G+YS+PPIPL  ACH+
Sbjct: 361 IFNQIKDGSYSDPPIPLNKACHK 383

BLAST of Tan0016302 vs. ExPASy TrEMBL
Match: A0A6J1HP45 (GDSL esterase/lipase At3g26430 OS=Cucurbita maxima OX=3661 GN=LOC111466427 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 4.5e-198
Identity = 325/384 (84.64%), Postives = 355/384 (92.45%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           MECH LMIA     AL L+P SVLAR+PCNFPAVFNFGDSNSDTGGLSA++GQAPPPNGM
Sbjct: 1   MECHSLMIA---VFALLLVPHSVLARKPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGM 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           SYF GPAGRYCDGRL++DFIA+SLGLPYLSAYL+ALGANF+HGANFATAGSTVR QNRT+
Sbjct: 61  SYFHGPAGRYCDGRLVIDFIAQSLGLPYLSAYLNALGANFSHGANFATAGSTVRPQNRTL 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
           QQSGFSPISL+VQ+NEFYDFRRRSQTVRNR GG F+ LLP+E +F +ALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLDVQYNEFYDFRRRSQTVRNRLGGFFKPLLPKETSFGQALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           SGYFANMTV+QVR+YIP+VLDQFS IVKWVH QGGRFFWIHNTGPVGCLPYVLDR PVS 
Sbjct: 181 SGYFANMTVEQVRDYIPQVLDQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRMPVSP 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           SDYDQYGCA PFNDLAQ+FNRGLK  VVELRAALPDAA+TYVD+YSLKY L+SQHKK+GF
Sbjct: 241 SDYDQYGCAIPFNDLAQTFNRGLKDAVVELRAALPDAALTYVDIYSLKYDLVSQHKKYGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
           EFPLRTCCGHGGKYNFNV LGC G+K +NG DVLIGKAC+KP+VYVNWDGVH TQAANKW
Sbjct: 301 EFPLRTCCGHGGKYNFNVKLGCAGKKRMNGKDVLIGKACEKPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHRA 385
           IFN IKTGAYS+PPIPL+MACHRA
Sbjct: 361 IFNHIKTGAYSDPPIPLEMACHRA 381

BLAST of Tan0016302 vs. ExPASy TrEMBL
Match: A0A5A7SSS5 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001540 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 5.0e-197
Identity = 322/383 (84.07%), Postives = 355/383 (92.69%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           ME H L+IAFTTFLALSLIP   LA+ PCNFPAVFNFGDSNSDTGGLSA++GQAPPPNG 
Sbjct: 1   MESHSLIIAFTTFLALSLIPCPSLAKHPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           SYF GPAGRY DGRLIVDFIA+SLGLPYLSAYLDALGANF+HGANFATAGST+R+QNRT+
Sbjct: 61  SYFPGPAGRYSDGRLIVDFIAESLGLPYLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
           QQSGFSPISLNVQ+NEFYDFRRRSQT+RN  GG+F+QLLP+E++FSRALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           SGYFANM++ QV+ YIP+VL QFS IVKWVH QGGRFFWIHNTGPVGCLPYVLDR+PV A
Sbjct: 181 SGYFANMSLHQVKLYIPDVLQQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREPVPA 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           SDYDQYGCATPFNDLAQ FNRGLKQ V+ELR ALPD+AITYVDVYSLKYAL+SQHKK+GF
Sbjct: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVMELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
           E+PLRTCCGHGGKYNFNV LGCGG+K ING +VLIGK+CK P+VYVNWDGVH TQAANKW
Sbjct: 301 EYPLRTCCGHGGKYNFNVKLGCGGKKTINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHR 384
           IFNQIK G+YS+PPIPL  ACH+
Sbjct: 361 IFNQIKDGSYSDPPIPLNKACHK 383

BLAST of Tan0016302 vs. ExPASy TrEMBL
Match: A0A1S3BF19 (GDSL esterase/lipase At3g26430 OS=Cucumis melo OX=3656 GN=LOC103488959 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 5.0e-197
Identity = 322/383 (84.07%), Postives = 355/383 (92.69%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           ME H L+IAFTTFLALSLIP   LA+ PCNFPAVFNFGDSNSDTGGLSA++GQAPPPNG 
Sbjct: 1   MESHSLIIAFTTFLALSLIPCPSLAKHPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           SYF GPAGRY DGRLIVDFIA+SLGLPYLSAYLDALGANF+HGANFATAGST+R+QNRT+
Sbjct: 61  SYFPGPAGRYSDGRLIVDFIAESLGLPYLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
           QQSGFSPISLNVQ+NEFYDFRRRSQT+RN  GG+F+QLLP+E++FSRALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           SGYFANM++ QV+ YIP+VL QFS IVKWVH QGGRFFWIHNTGPVGCLPYVLDR+PV A
Sbjct: 181 SGYFANMSLHQVKLYIPDVLQQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREPVPA 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           SDYDQYGCATPFNDLAQ FNRGLKQ V+ELR ALPD+AITYVDVYSLKYAL+SQHKK+GF
Sbjct: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVMELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
           E+PLRTCCGHGGKYNFNV LGCGG+K ING +VLIGK+CK P+VYVNWDGVH TQAANKW
Sbjct: 301 EYPLRTCCGHGGKYNFNVKLGCGGKKTINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHR 384
           IFNQIK G+YS+PPIPL  ACH+
Sbjct: 361 IFNQIKDGSYSDPPIPLNKACHK 383

BLAST of Tan0016302 vs. ExPASy TrEMBL
Match: A0A0A0KS60 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G598030 PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 9.4e-196
Identity = 320/383 (83.55%), Postives = 354/383 (92.43%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           ME H L+IAFTTFLALSL P   LA++PCNFPAVFNFGDSNSDTGGLSA++GQAPPPNG 
Sbjct: 1   MESHSLIIAFTTFLALSLTPWPALAKDPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGR 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           SYF GPAGRY DGRLIVDFIA+S GLP+LSAYLDALGANF+HGANFATAGST+R+QNRT+
Sbjct: 61  SYFPGPAGRYSDGRLIVDFIAESFGLPHLSAYLDALGANFSHGANFATAGSTIRLQNRTL 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
           QQSGFSPISLNVQ+NEFYDFRRRSQT+RN  GG+F+QLLP+E++FSRALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLNVQYNEFYDFRRRSQTLRNGLGGIFKQLLPKEESFSRALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           SGYFANMT+ QV+ Y+P+VL QFS IVKWVH QGGRFFWIHNTGPVGCLPYVLDR+ V A
Sbjct: 181 SGYFANMTLHQVKLYVPDVLHQFSEIVKWVHSQGGRFFWIHNTGPVGCLPYVLDREHVPA 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           SDYDQYGCATPFNDLAQ FNRGLKQ VVELR ALPD+AITYVDVYSLKYAL+SQHKK+GF
Sbjct: 241 SDYDQYGCATPFNDLAQYFNRGLKQAVVELRKALPDSAITYVDVYSLKYALVSQHKKYGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
           E+PLRTCCGHGGKYNFNVNLGCGG+K ING +VLIGK+CK P+VYVNWDGVH TQAANKW
Sbjct: 301 EYPLRTCCGHGGKYNFNVNLGCGGKKEINGKEVLIGKSCKNPEVYVNWDGVHYTQAANKW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHR 384
           IFNQIK G+YS+PPIPL  ACH+
Sbjct: 361 IFNQIKDGSYSDPPIPLNKACHK 383

BLAST of Tan0016302 vs. ExPASy TrEMBL
Match: A0A6J1G253 (alpha-L-fucosidase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111449990 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.1e-195
Identity = 321/384 (83.59%), Postives = 352/384 (91.67%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           MEC  LMIA     AL L+P SVLAR+PCNFPAVFNFGDSNSDTGGLSA++GQAPPPNGM
Sbjct: 1   MECRSLMIA---VFALLLVPHSVLARKPCNFPAVFNFGDSNSDTGGLSAVFGQAPPPNGM 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           SYF GPAGRYCDGRL++DFIA+SLGLPYLSAYL+ALGANF+HGANFATAGSTVR QNRT+
Sbjct: 61  SYFHGPAGRYCDGRLVIDFIAESLGLPYLSAYLNALGANFSHGANFATAGSTVRPQNRTL 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
           QQSGFSPISL+VQ+NEFYDFRRRSQTVRNR GG F+ LLP+E +F RALYTFDIGQNDLT
Sbjct: 121 QQSGFSPISLDVQYNEFYDFRRRSQTVRNRLGGFFKPLLPKETSFGRALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           SGYFANMTV+QVR+YIP+VL+QFS IVKWVH QGGRFFWIHNTGPVGCLPYVLDR PVS 
Sbjct: 181 SGYFANMTVEQVRDYIPQVLNQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRMPVSP 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           SDYDQYGCA PFNDLAQ+FN GLK  VV+LRAALP+A +TYVD+YSLKY L+SQHKKHGF
Sbjct: 241 SDYDQYGCAIPFNDLAQTFNHGLKDAVVKLRAALPNATLTYVDIYSLKYDLVSQHKKHGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
           EFPLRTCCGHGGKYNFNV LGC G+K +NG DVLIGKACKKP+VYVNWDGVH TQAAN W
Sbjct: 301 EFPLRTCCGHGGKYNFNVKLGCAGKKRMNGKDVLIGKACKKPEVYVNWDGVHYTQAANNW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHRA 385
           IFNQIKTGAYS+PPIPL+MAC+RA
Sbjct: 361 IFNQIKTGAYSDPPIPLEMACYRA 381

BLAST of Tan0016302 vs. TAIR 10
Match: AT1G67830.1 (alpha-fucosidase 1 )

HSP 1 Score: 492.7 bits (1267), Expect = 2.8e-139
Identity = 231/374 (61.76%), Postives = 289/374 (77.27%), Query Frame = 0

Query: 10  FTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGMSYFRGPAGR 69
           F   L  SL PS+      C+FPA+FNFGDSNSDTGGLSA +GQA PP+G S+F  PAGR
Sbjct: 9   FALSLLSSLSPST--HAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGR 68

Query: 70  YCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTIQQSGFSPIS 129
           YCDGRL++DFIA+SLGLPYLSA+LD++G+NF+HGANFATAGS +R  N T++QSGFSP S
Sbjct: 69  YCDGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFS 128

Query: 130 LNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLTSGYFANMTV 189
           L+VQF +FY+F  RSQTVR+R GGV++ +LPE D+FS+ALYTFDIGQNDLT+GYFAN TV
Sbjct: 129 LDVQFVQFYNFHNRSQTVRSR-GGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFANKTV 188

Query: 190 DQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSASDYDQYGCA 249
           +QV   +PE++ QF N +K ++GQGGR+FWIHNTGP+GCL YV++R P  ASD+D +GC 
Sbjct: 189 EQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCV 248

Query: 250 TPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGFEFPLRTCCG 309
           +P N LAQ FN  LKQ V+ELR++L +AAITYVDVYSLK+ L    + HGF+  L +CCG
Sbjct: 249 SPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCG 308

Query: 310 HGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKWIFNQIKTGA 369
           HGGKYN+N  +GCG +K + G +V IGK C +P   V WDGVH TQAANK+IF++I  G 
Sbjct: 309 HGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG- 368

Query: 370 YSEPPIPLKMACHR 384
                  L  AC R
Sbjct: 369 -------LSKACKR 371

BLAST of Tan0016302 vs. TAIR 10
Match: AT3G26430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 482.3 bits (1240), Expect = 3.7e-136
Identity = 223/383 (58.22%), Postives = 288/383 (75.20%), Query Frame = 0

Query: 1   MECHFLMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGM 60
           ME + L++      +  + P +      CNFPA+FNFGDSNSDTGGLSA +GQAP PNG 
Sbjct: 1   METNLLLVKCVLLASCLIHPRA--CSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQ 60

Query: 61  SYFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTI 120
           ++F  P+GR+ DGRLI+DFIA+ LGLPYL+A+LD++G+NF+HGANFATAGSTVR  N TI
Sbjct: 61  TFFHSPSGRFSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATI 120

Query: 121 QQSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLT 180
            QSG SPISL+VQ  +F DF  RSQ +RNR GGVF++LLP+++ FS+ALYTFDIGQNDLT
Sbjct: 121 AQSGVSPISLDVQLVQFSDFITRSQLIRNR-GGVFKKLLPKKEYFSQALYTFDIGQNDLT 180

Query: 181 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 240
           +G   NMT DQ++ YIP+V DQ SN+++ V+ +GGR FWIHNT P+GCLPYVLDR PV A
Sbjct: 181 AGLKLNMTSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPA 240

Query: 241 SDYDQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGF 300
           S  D +GCA P N++A+ +N  LK+ V+ELR  L +AA TYVD+YS+K  LI+Q KK GF
Sbjct: 241 SQIDNHGCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGF 300

Query: 301 EFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKW 360
            +PL  CCGHGGKYNFN  + CG +  I G ++++ K+C      V+WDG+H T+  N W
Sbjct: 301 RYPLVACCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSW 360

Query: 361 IFNQIKTGAYSEPPIPLKMACHR 384
           IF QI  GA+S+PP+P+K AC R
Sbjct: 361 IFQQINDGAFSDPPLPVKSACTR 380

BLAST of Tan0016302 vs. TAIR 10
Match: AT5G14450.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 369.4 bits (947), Expect = 3.5e-102
Identity = 183/384 (47.66%), Postives = 256/384 (66.67%), Query Frame = 0

Query: 6   LMIAFTTF----LALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGMS 65
           LM++ T F    L L  + +SV  +  C FPA++NFGDSNSDTGG+SA +     P G  
Sbjct: 10  LMVSSTVFSWLLLCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQG 69

Query: 66  YFRGPAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTIQ 125
           +F  P GR  DGRL +DFIA+ LGLPYLSAYL++LG+NF HGANFAT GST+R QN TI 
Sbjct: 70  FFHRPTGRDSDGRLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQNETIF 129

Query: 126 QSGFSPISLNVQFNEFYDFRRRSQTVRNRFGGVFE-QLLPEEDTFSRALYTFDIGQNDLT 185
           Q G SP SL++Q  +F  F+ RS  +  +    ++ + LP ++ F++ALYTFDIGQNDL+
Sbjct: 130 QYGISPFSLDMQIAQFDQFKARSALLFTQIKSRYDREKLPRQEEFAKALYTFDIGQNDLS 189

Query: 186 SGYFANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSA 245
            G F  M+VDQ++  IP++++  ++ V+ ++ QGGR FW+HNTGP GCLP  +      A
Sbjct: 190 VG-FRTMSVDQLKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPA 249

Query: 246 SDY-DQYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHG 305
             Y D+ GC    N++A  FNR LK+TV+ LR  L  AAITYVDVY+ KY ++S  KK G
Sbjct: 250 PGYLDKSGCVKAQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLG 309

Query: 306 FEFPLRTCCGHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANK 365
           F  PL+ CCG+  KY+   ++ CG +  +N T++  G +C  P + V+WDGVH T+AANK
Sbjct: 310 FANPLKVCCGYHEKYD---HIWCGNKGKVNNTEI-YGGSCPNPVMAVSWDGVHYTEAANK 369

Query: 366 WIFNQIKTGAYSEPPIPLKMACHR 384
            + ++   G  ++PP+P+  AC+R
Sbjct: 370 HVADRTLNGLLTDPPVPITRACYR 388

BLAST of Tan0016302 vs. TAIR 10
Match: AT3G27950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.5e-89
Identity = 173/377 (45.89%), Postives = 239/377 (63.40%), Query Frame = 0

Query: 6   LMIAFTTFLALSLIPSSVLAREPCNFPAVFNFGDSNSDTGGLSALYGQAPPPNGMSYFRG 65
           L++ FT    LS + SS      CNFPAVFNFGDSNSDTG +SA  G+ PPPNG+++F  
Sbjct: 13  LLLGFTE--KLSALSSS------CNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGR 72

Query: 66  PAGRYCDGRLIVDFIAKSLGLPYLSAYLDALGANFTHGANFATAGSTVRVQNRTIQQSGF 125
            AGR+ DGRLI+DFI ++L LPYL+ YLD++GAN+ HGANFAT GS +R        + F
Sbjct: 73  SAGRHSDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIRP-----TLACF 132

Query: 126 SPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLTSGYFA 185
           SP  L  Q ++F  F+ R+ ++ N           + + FS+ALYT DIGQNDL  G F 
Sbjct: 133 SPFHLGTQVSQFIHFKTRTLSLYN-----------QTNDFSKALYTLDIGQNDLAIG-FQ 192

Query: 186 NMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSASDYDQ 245
           NMT +Q++  IP +++ F+  +K ++ +G RFF IHNTGP GCLPY+L  K   A   D 
Sbjct: 193 NMTEEQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLPYLL--KAFPAIPRDP 252

Query: 246 YGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGFEFPLR 305
           YGC  P N++A  FN+ LK  + +L+  LP +  TYVDVYS KY LI++ K  GF  P  
Sbjct: 253 YGCLKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFD 312

Query: 306 TCC-GHGGKYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKWIFNQ 365
            CC G  G+      +GCG   ++NGT+ L   +C+  K +++WDG+H T+ AN  + N+
Sbjct: 313 YCCVGAIGR-----GMGCGKTIFLNGTE-LYSSSCQNRKNFISWDGIHYTETANMLVANR 356

Query: 366 IKTGAYSEPPIPLKMAC 382
           I  G+ S+PP+P + AC
Sbjct: 373 ILDGSISDPPLPTQKAC 356

BLAST of Tan0016302 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.3e-83
Identity = 162/376 (43.09%), Postives = 232/376 (61.70%), Query Frame = 0

Query: 8   IAFTTFLALSL-IPSSVLAREPCNFPAVFNFGDSNSDTGGL-SALYGQAPPPNGMSYFRG 67
           ++   F+ +SL +PSS        +PA+ NFGDSNSDTG L SA      PP G +YF  
Sbjct: 8   LSIVLFILISLFLPSSFSI--ILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNL 67

Query: 68  PAGRYCDGRLIVDFIAKSLGLPYLSAYLDALG-ANFTHGANFATAGSTVRVQNRTIQQSG 127
           P+GRYCDGRLIVDF+   + LP+L+ YLD+LG  NF  G NFA AGST+   N T     
Sbjct: 68  PSGRYCDGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPT----S 127

Query: 128 FSPISLNVQFNEFYDFRRRSQTVRNRFGGVFEQLLPEEDTFSRALYTFDIGQNDLTSGYF 187
            SP S ++Q ++F  F+ R+  + ++ G  +E+ LP  D +S+ LY  DIGQND+ +G F
Sbjct: 128 VSPFSFDLQISQFIRFKSRAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDI-AGAF 187

Query: 188 ANMTVDQVREYIPEVLDQFSNIVKWVHGQGGRFFWIHNTGPVGCLPYVLDRKPVSASDYD 247
            + T+DQV   IP +L+ F   +K ++ +GGR  WIHNTGP+GCL   + +    ++  D
Sbjct: 188 YSKTLDQVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLD 247

Query: 248 QYGCATPFNDLAQSFNRGLKQTVVELRAALPDAAITYVDVYSLKYALISQHKKHGFEFPL 307
           ++GC +  N  A+ FN  L     + +A  PDA +TYVD++S+K  LI+ + + GFE PL
Sbjct: 248 EFGCVSSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPL 307

Query: 308 RTCCGHGG-KYNFNVNLGCGGRKWINGTDVLIGKACKKPKVYVNWDGVHLTQAANKWIFN 367
             CCG GG   N++  + CG  K ++G  V   KAC     Y+NWDG+H T+AAN+++ +
Sbjct: 308 MACCGVGGAPLNYDSRITCGQTKVLDGISV-TAKACNDSSEYINWDGIHYTEAANEFVSS 367

Query: 368 QIKTGAYSEPPIPLKM 380
           QI TG YS+PP   +M
Sbjct: 368 QILTGKYSDPPFSDQM 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FXE53.9e-13861.76Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1[more]
Q9LIN25.3e-13558.22GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1... [more]
B4FZ874.9e-13357.94GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1[more]
Q7Y1X19.6e-11353.70Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1[more]
Q9LY845.0e-10147.66GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2... [more]
Match NameE-valueIdentityDescription
XP_038891379.18.7e-20487.31GDSL esterase/lipase At3g26430 [Benincasa hispida][more]
XP_023541593.17.2e-19884.90alpha-L-fucosidase 3-like [Cucurbita pepo subsp. pepo][more]
XP_022966842.19.4e-19884.64GDSL esterase/lipase At3g26430 [Cucurbita maxima][more]
XP_008446147.11.0e-19684.07PREDICTED: GDSL esterase/lipase At3g26430 [Cucumis melo] >KAA0034264.1 GDSL este... [more]
XP_004135278.12.0e-19583.55GDSL esterase/lipase At3g26430 [Cucumis sativus] >KGN51744.1 hypothetical protei... [more]
Match NameE-valueIdentityDescription
A0A6J1HP454.5e-19884.64GDSL esterase/lipase At3g26430 OS=Cucurbita maxima OX=3661 GN=LOC111466427 PE=3 ... [more]
A0A5A7SSS55.0e-19784.07GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G... [more]
A0A1S3BF195.0e-19784.07GDSL esterase/lipase At3g26430 OS=Cucumis melo OX=3656 GN=LOC103488959 PE=3 SV=1[more]
A0A0A0KS609.4e-19683.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G598030 PE=3 SV=1[more]
A0A6J1G2532.1e-19583.59alpha-L-fucosidase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111449990 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G67830.12.8e-13961.76alpha-fucosidase 1 [more]
AT3G26430.13.7e-13658.22GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G14450.13.5e-10247.66GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G27950.11.5e-8945.89GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G54790.31.3e-8343.09GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 22..368
e-value: 5.6E-75
score: 254.7
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 34..365
e-value: 1.8E-46
score: 159.4
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 27..383
NoneNo IPR availablePANTHERPTHR22835:SF117BNAA06G32900D PROTEINcoord: 27..383
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 35..365
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 32..365
e-value: 1.58896E-117
score: 342.288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0016302.1Tan0016302.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds