Tan0016236 (gene) Snake gourd v1

Overview
NameTan0016236
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionDelta(24)-sterol reductase
LocationLG04: 82044922 .. 82049061 (+)
RNA-Seq ExpressionTan0016236
SyntenyTan0016236
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGTTCATTTTCTTTTTGCGGTATTTAATATATTGTCCCGCTTTCATTGCTTTGCTGTCTGAATCAGTTAAAATCTAGCCATTTGGCTACTACTCTCATTCTGCTTCAATCCTTACGTTTTCATCATCATTTTTCAATCAGATTCTTTGCTAGTTTGTGTAATCTTTTCAACCTTTCTCTGCAGAATTTGTAGTCTCAGTTCGGTATTTTCTGGGTCAGTTCTGTTTCTCACTTGCTTGTTTCTCACTTTCTTCACTTGAATCGTGTTTGGTTGCTGAGAAAATCGGTTTTCTCGTGATGGGGTTGTTAGTTCTTGTGATTTTTCGCTTTTTAACCTTTTTTTTTTTGACGATCGATGCTTGTTTATCAGTTTTTGGGGGCTTGTTCTCTCTGTTTTGATTCTGGGGGTGAAAGAAAGGGGGACTTTGGGGGGTTAAGAGTTGGGTTTGATTTTCCGGGATAATGCTTGAGATCCTGCGAGAGAAAGTTTTGGAGAGTTAACTTTGTGCATTACACAGTGAAATTTCCTTTTTCTCTAATCTGTTTCTCATTGATTCTTGTGCTTTTTTTTTTGTTTGTCCTTTTTATCATTTTTGAAGTGAAATGTCGTTTTGAACTTTTGCCCAACTTTCCATATGGATGGCTTTTGTTTTCCCCTTGAACTGCACATGTCTCATTAATTGCTTCTATCGTTTTCATTTAAAGATGTGGCCATTTCCAGCTATTTTTGGCCATTTTCCACCATTTCTATCTGTTGTGTAAGCCATAGATGGTTCTTTATGTTTGGTTGTTTGAGTTAGTTTATATTGCCTTTGGAATGTTGCTTTGCTGCTAGTGGAAAGTTTTTGCCCCTTTTGCCAGTCCCTTTGAACTCTTTGGTATTTTTCATGTAGAGTGTATAAGGACTTTACATGCAGAGAGCTTTCTCTTCTTTGATATCATGAACCTGTTTTGATGTTTTTTTTCTTGTGTTTGATCATAGAACTTGTTCTAGATCCTTTAATCCACGAACTTTGACGATAATCTTAAGCCTCGAGTTCCACGTTTTAGATAACAAATCAACAGCATCTTTCTGTTTCCTTAAATGGTCACTTATTTTCAAGCAGTCTGGGAACTTCATGTTTTGTATGGTTCAGAAATTAGAGTCCAGCCTTAGTTGTGTTGTTCTTCAACTCAAGATAGCACTTCTCATGAATGGAGCAATGGACTAATAAAAGATGATGTACATAATAGCTCTCAAAATTGAATCGAGGAAAAAGAAACTTCCCTGGACATCTAAGCTCATTCAATAGTTTGCTTTGCTTATTATTCTGAGGTTGAGTTTGATTAGGATGTTTGTAGTTTGTACCCTGTTGTTAGATTTCAAATAGTTGTATTGTAACGAGTACAACTTAGTTAACTAAAGAAGGTTTGGTTCCACTTTTCTTCCTTGGTTTTTCTTTTTCCAACTTTAGACACTTTCATGCCTACTAATTTTTTGATCTATCAAATATTCATCTTGCAAAGCCAAGAATACCTTTCTGTGCAGGGTAATTTCAGTTTAAGTAGAACACTTGTAGTTTATTTACATCTTCATAACATGTTGGCTTGTTTGGATCATCTACTGACATGTTTTGGATTGCTATGTCATTTGGAAGTAATGGAATGTTTGTTTGTTTTGTTCTGAAATAGTTTTCCGGTTTTGTTACACACGAAATAATTTTCGTTTTTATTTTCTTTGCAATTTCGTGATCAAGTTAGGGTTGCTATAACTTGTAGTAAATGATGAAGAGTTTGTGAAAGCTAATTCACTTATTATAAATTTATGACTTTTACCTGTTTTTCTCAGGAGATAAGCATCTTGATTCGCCATAATGTCAGACCTCGAGGCCTCCAAGCCACAGAAGAGGAAGAAAGGCTGGGTGGACCTCTTGGTTAATTTTCGATGGATTATAGTCATTTTTGTAGTTCTTCCGTTCTCATTCAGTTTCTACTTCATGCAATATCTTGGGGACAAACGATCTGAGAGGAAGTCCTACAAGCAACGGCAGATCGAACACGAAGAAAATGTCCAGAAAGTTGTCGATCGCCTCAAACAAAGAAACCCTTTGAAGGATGGTCTTGTCTGCTCTGCCCGGAAGCCTTGGCTTGCAGTGGGAATGCGAAATGTTGATTACAAACGAGCACGACACTTTGAGGTTGATCTGTCGCCTTTCCGAAACATACTTGACATTGACAAGGACAGAATGATTGCTAAGGTTGAGCCCCATGTGAACATGGGGCAAATATCTAGAGCCACAATTCCCATGAACCTTTCACTTGCGGTAGTTGCTGAGCTCGATGATCTTACTGTTGGTGGCCTCATCAATGGCTATGGCATTGAGGGAAGCTCCCATATCTATGGCCTTTTCTCTGATACTGTTGTGGCCTATGAAATAATTCTTGCAGACGGCAGGGTCGTAAGGGCGACCAAGGATAACGACTACTCTGATCTTTTCTATGCCATCCCATGGTCTCAAGGAACTCTGGGTTTTCTTGTGTCTGCTGAGATCAAGCTTATAGATATAAAGGAATATATGAAGCTTACATACAAACCTGCCAAGGGCAACCTACAGGAGATTGGGCAGGCATATATGGACTCATTTACACCTAGAGATTTAGATCAAGATAATCCTGAGAAGGTTCCCGACTTCGTAGAAACCATGATTTATTCTCCTACCGAAGCCGTGTGCATGACAGGAAGATATGCATCCAAAGAAGAGGCCAAGAAAAAAGGGAATGTGATCAACAGCATTGGCTGGTGGTTCAAGCCCTGGTTTTACCAGCATGCACAAAAAGCACTGAAAAAAGGGGAGTTTGTGGAATATATCCCAACAAGAGAGTACTATCACAGGCACACCAGATCTTTGTACTGGGAGGGAAAGCTCATCCTTCCGTTTGCCGATCAATGGTGGTTCCGGTTTCTCTTGGGATGGATGATGCCTCCCAAGGTCTCTCTACTAAAGGCTACTCAAGGTGAGGCTATCAGAAACTATTACCATGAGATGCATATTGTTCAGGACATGCTCGTTCCTCTTCGAAGGATCGGGGACACGTTGGAGTGGATCGACAGGGAGATGGAGGTAATTTTCTTCTTGTCCTTTGAACTTATTTATAATAGTCCTAACTCCTATATTAGCTTGAACGTTAGATACCAAAAATAAAGTTCTATTTCTAGTAACATGCCACAAGGAAGAACAAGATTTGGTCATTTTCTTGGGATGGTTTTCTGGTTAAATAAATTATGGTTGTTGTTTACTTGCAATTCAAATGAGAATTTGGGATGTTTGGACTGTTGATTGGTTACAGAAATACTATAAAACCAGGAGTCTCCACTCATTTGCTGTATGTTTTGGCACCCCTTTCTTAAATCTTGTCATTACTGAATCATTTGCAGGTGTACCCATTATGGCTCTGCCCACACAGATTGTTCAAGCCCCCAATGAGAACAATGGTGTCTCCTGAACCAGGATTTGAGCTGCAACTCAAGCAGGGCGACACCCCCTACGCTCAGATGTACACAGACATCGGAGTCTACTACGCTCCAGGGCCCGTCCTGAGGGGCGAAGTTTACGACGGTGCTCAGGCAGTTCGCAACATGGAGAGCTGGCTTATTGAAATCCATGGTTTCCAACCACAGCACTCAGTGACTGAGATGAGCGAGAAAGAGTTTTGGAAAATGTTCGATCCCGAACTTTACGAGGTGTGCCGAAAGAAGTACGGAGCCATAGGGACCTTTATGCATGTGTTTTACAAATGTAAGAAGGGGAAGAAGACTGAGAAAGAAGTCCAGGAAGCTGAACAAGCTCAAGCTGAAACTGCTTATGCTGATATAGAGCAGCCAGCTGAGTGATGCTTGTGCATTGGCAAACAAAGTAGCATTCTTGTTTCTCCCCACTTTGTTTCTGTTTTCTTTGTATTTAATCTTTTTCTTTGAAATTTCACTGTGCTCATTAGATTCAAATTTGTGGTAAGAGAGGTTGTTGCCTTCTCTCTCTCTCTCTGTATCTTTCACTTGGTCTCTTAAACTCAGAAAATTATTTTCATGTGTTTGTTGATTATGGTATATGTATTCTATATTGTAATGTCAAAACTGAAAATAATGATAGAGTAAATTGCAC

mRNA sequence

AAAGTTCATTTTCTTTTTGCGGTATTTAATATATTGTCCCGCTTTCATTGCTTTGCTGTCTGAATCAGTTAAAATCTAGCCATTTGGCTACTACTCTCATTCTGCTTCAATCCTTACGTTTTCATCATCATTTTTCAATCAGATTCTTTGCTAGTTTGTGTAATCTTTTCAACCTTTCTCTGCAGAATTTGTAGTCTCAGTTCGGTATTTTCTGGGAGATAAGCATCTTGATTCGCCATAATGTCAGACCTCGAGGCCTCCAAGCCACAGAAGAGGAAGAAAGGCTGGGTGGACCTCTTGGTTAATTTTCGATGGATTATAGTCATTTTTGTAGTTCTTCCGTTCTCATTCAGTTTCTACTTCATGCAATATCTTGGGGACAAACGATCTGAGAGGAAGTCCTACAAGCAACGGCAGATCGAACACGAAGAAAATGTCCAGAAAGTTGTCGATCGCCTCAAACAAAGAAACCCTTTGAAGGATGGTCTTGTCTGCTCTGCCCGGAAGCCTTGGCTTGCAGTGGGAATGCGAAATGTTGATTACAAACGAGCACGACACTTTGAGGTTGATCTGTCGCCTTTCCGAAACATACTTGACATTGACAAGGACAGAATGATTGCTAAGGTTGAGCCCCATGTGAACATGGGGCAAATATCTAGAGCCACAATTCCCATGAACCTTTCACTTGCGGTAGTTGCTGAGCTCGATGATCTTACTGTTGGTGGCCTCATCAATGGCTATGGCATTGAGGGAAGCTCCCATATCTATGGCCTTTTCTCTGATACTGTTGTGGCCTATGAAATAATTCTTGCAGACGGCAGGGTCGTAAGGGCGACCAAGGATAACGACTACTCTGATCTTTTCTATGCCATCCCATGGTCTCAAGGAACTCTGGGTTTTCTTGTGTCTGCTGAGATCAAGCTTATAGATATAAAGGAATATATGAAGCTTACATACAAACCTGCCAAGGGCAACCTACAGGAGATTGGGCAGGCATATATGGACTCATTTACACCTAGAGATTTAGATCAAGATAATCCTGAGAAGGTTCCCGACTTCGTAGAAACCATGATTTATTCTCCTACCGAAGCCGTGTGCATGACAGGAAGATATGCATCCAAAGAAGAGGCCAAGAAAAAAGGGAATGTGATCAACAGCATTGGCTGGTGGTTCAAGCCCTGGTTTTACCAGCATGCACAAAAAGCACTGAAAAAAGGGGAGTTTGTGGAATATATCCCAACAAGAGAGTACTATCACAGGCACACCAGATCTTTGTACTGGGAGGGAAAGCTCATCCTTCCGTTTGCCGATCAATGGTGGTTCCGGTTTCTCTTGGGATGGATGATGCCTCCCAAGGTCTCTCTACTAAAGGCTACTCAAGGTGAGGCTATCAGAAACTATTACCATGAGATGCATATTGTTCAGGACATGCTCGTTCCTCTTCGAAGGATCGGGGACACGTTGGAGTGGATCGACAGGGAGATGGAGGTGTACCCATTATGGCTCTGCCCACACAGATTGTTCAAGCCCCCAATGAGAACAATGGTGTCTCCTGAACCAGGATTTGAGCTGCAACTCAAGCAGGGCGACACCCCCTACGCTCAGATGTACACAGACATCGGAGTCTACTACGCTCCAGGGCCCGTCCTGAGGGGCGAAGTTTACGACGGTGCTCAGGCAGTTCGCAACATGGAGAGCTGGCTTATTGAAATCCATGGTTTCCAACCACAGCACTCAGTGACTGAGATGAGCGAGAAAGAGTTTTGGAAAATGTTCGATCCCGAACTTTACGAGGTGTGCCGAAAGAAGTACGGAGCCATAGGGACCTTTATGCATGTGTTTTACAAATGTAAGAAGGGGAAGAAGACTGAGAAAGAAGTCCAGGAAGCTGAACAAGCTCAAGCTGAAACTGCTTATGCTGATATAGAGCAGCCAGCTGAGTGATGCTTGTGCATTGGCAAACAAAGTAGCATTCTTGTTTCTCCCCACTTTGTTTCTGTTTTCTTTGTATTTAATCTTTTTCTTTGAAATTTCACTGTGCTCATTAGATTCAAATTTGTGGTAAGAGAGGTTGTTGCCTTCTCTCTCTCTCTCTGTATCTTTCACTTGGTCTCTTAAACTCAGAAAATTATTTTCATGTGTTTGTTGATTATGGTATATGTATTCTATATTGTAATGTCAAAACTGAAAATAATGATAGAGTAAATTGCAC

Coding sequence (CDS)

ATGTCAGACCTCGAGGCCTCCAAGCCACAGAAGAGGAAGAAAGGCTGGGTGGACCTCTTGGTTAATTTTCGATGGATTATAGTCATTTTTGTAGTTCTTCCGTTCTCATTCAGTTTCTACTTCATGCAATATCTTGGGGACAAACGATCTGAGAGGAAGTCCTACAAGCAACGGCAGATCGAACACGAAGAAAATGTCCAGAAAGTTGTCGATCGCCTCAAACAAAGAAACCCTTTGAAGGATGGTCTTGTCTGCTCTGCCCGGAAGCCTTGGCTTGCAGTGGGAATGCGAAATGTTGATTACAAACGAGCACGACACTTTGAGGTTGATCTGTCGCCTTTCCGAAACATACTTGACATTGACAAGGACAGAATGATTGCTAAGGTTGAGCCCCATGTGAACATGGGGCAAATATCTAGAGCCACAATTCCCATGAACCTTTCACTTGCGGTAGTTGCTGAGCTCGATGATCTTACTGTTGGTGGCCTCATCAATGGCTATGGCATTGAGGGAAGCTCCCATATCTATGGCCTTTTCTCTGATACTGTTGTGGCCTATGAAATAATTCTTGCAGACGGCAGGGTCGTAAGGGCGACCAAGGATAACGACTACTCTGATCTTTTCTATGCCATCCCATGGTCTCAAGGAACTCTGGGTTTTCTTGTGTCTGCTGAGATCAAGCTTATAGATATAAAGGAATATATGAAGCTTACATACAAACCTGCCAAGGGCAACCTACAGGAGATTGGGCAGGCATATATGGACTCATTTACACCTAGAGATTTAGATCAAGATAATCCTGAGAAGGTTCCCGACTTCGTAGAAACCATGATTTATTCTCCTACCGAAGCCGTGTGCATGACAGGAAGATATGCATCCAAAGAAGAGGCCAAGAAAAAAGGGAATGTGATCAACAGCATTGGCTGGTGGTTCAAGCCCTGGTTTTACCAGCATGCACAAAAAGCACTGAAAAAAGGGGAGTTTGTGGAATATATCCCAACAAGAGAGTACTATCACAGGCACACCAGATCTTTGTACTGGGAGGGAAAGCTCATCCTTCCGTTTGCCGATCAATGGTGGTTCCGGTTTCTCTTGGGATGGATGATGCCTCCCAAGGTCTCTCTACTAAAGGCTACTCAAGGTGAGGCTATCAGAAACTATTACCATGAGATGCATATTGTTCAGGACATGCTCGTTCCTCTTCGAAGGATCGGGGACACGTTGGAGTGGATCGACAGGGAGATGGAGGTGTACCCATTATGGCTCTGCCCACACAGATTGTTCAAGCCCCCAATGAGAACAATGGTGTCTCCTGAACCAGGATTTGAGCTGCAACTCAAGCAGGGCGACACCCCCTACGCTCAGATGTACACAGACATCGGAGTCTACTACGCTCCAGGGCCCGTCCTGAGGGGCGAAGTTTACGACGGTGCTCAGGCAGTTCGCAACATGGAGAGCTGGCTTATTGAAATCCATGGTTTCCAACCACAGCACTCAGTGACTGAGATGAGCGAGAAAGAGTTTTGGAAAATGTTCGATCCCGAACTTTACGAGGTGTGCCGAAAGAAGTACGGAGCCATAGGGACCTTTATGCATGTGTTTTACAAATGTAAGAAGGGGAAGAAGACTGAGAAAGAAGTCCAGGAAGCTGAACAAGCTCAAGCTGAAACTGCTTATGCTGATATAGAGCAGCCAGCTGAGTGA

Protein sequence

MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQIEHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDIDKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYKPAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPLWLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQAVRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKGKKTEKEVQEAEQAQAETAYADIEQPAE
Homology
BLAST of Tan0016236 vs. ExPASy Swiss-Prot
Match: P93472 (Delta(24)-sterol reductase OS=Pisum sativum OX=3888 GN=DIM PE=1 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 1.6e-276
Identity = 448/567 (79.01%), Postives = 508/567 (89.59%), Query Frame = 0

Query: 1   MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSDLEA    KRKK WVD  V FRWI+VIFVVLP SF+ YF+ YLGD RSE KS+K RQ 
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVKFRWILVIFVVLPISFTLYFLTYLGDVRSEWKSFKTRQK 60

Query: 61  EHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDI 120
           EH+ENVQKVV+RLK+RNP KDGLVC+ARKPW+AVGMRNVDYKRARHFEVDLSPFRNILDI
Sbjct: 61  EHDENVQKVVNRLKKRNPSKDGLVCTARKPWVAVGMRNVDYKRARHFEVDLSPFRNILDI 120

Query: 121 DKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIA+VEP VNMGQI+R T+PMNL+LAVVAELDDLTVGGLINGYGIEGSSH YGLFS
Sbjct: 121 DKERMIARVEPLVNMGQITRVTVPMNLALAVVAELDDLTVGGLINGYGIEGSSHKYGLFS 180

Query: 181 DTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYK 240
           DTVVA+EIILADG +V+ATKDN+YSDLFYAIPWSQGTLG LV+AE+KLI IKEYMKLTYK
Sbjct: 181 DTVVAFEIILADGSLVKATKDNEYSDLFYAIPWSQGTLGLLVAAEVKLIPIKEYMKLTYK 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           P  GNL++I QAY DSF PRD DQDN EKVPDFVETMIYSPT AVCMTGRYASKEEAKKK
Sbjct: 241 PVVGNLKDIAQAYSDSFAPRDGDQDNDEKVPDFVETMIYSPTRAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GN IN++GWW+K WFYQHA+ ALKKG FVEYIPTREYYHRHTR LYWEGKLILPF DQ+ 
Sbjct: 301 GNKINNVGWWYKTWFYQHAETALKKGLFVEYIPTREYYHRHTRCLYWEGKLILPFGDQFS 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPL 420
           FRFL GW+MPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW DREME+YP+
Sbjct: 361 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWSDREMEIYPI 420

Query: 421 WLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480
           WLCPH+LFK P++TM+ PE GFELQ +QGDT  AQM+TD+GVYYAPGPVLRGEV+DGA+A
Sbjct: 421 WLCPHKLFKLPIKTMIYPEAGFELQRRQGDTQNAQMFTDVGVYYAPGPVLRGEVFDGAEA 480

Query: 481 VRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKG 540
           VR MESW+IE H FQPQ++V+E++EK FW+MFD  LYE CR+KYGA+GTFM V+YKCKKG
Sbjct: 481 VRKMESWMIENHCFQPQYAVSELNEKNFWRMFDAGLYEHCRRKYGAVGTFMSVYYKCKKG 540

Query: 541 KKTEKEVQEAEQAQAETAYADIEQPAE 568
           +KTEKEV+EAEQA  +TAY +++QP +
Sbjct: 541 RKTEKEVREAEQAHLDTAYPEVDQPPD 567

BLAST of Tan0016236 vs. ExPASy Swiss-Prot
Match: Q39085 (Delta(24)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=DIM PE=1 SV=2)

HSP 1 Score: 891.3 bits (2302), Expect = 5.5e-258
Identity = 418/564 (74.11%), Postives = 490/564 (86.88%), Query Frame = 0

Query: 1   MSDLEASKPQ-KRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQ 60
           MSDL+    + KRKK WVD  V FRWIIVIF+VLPFS +FYF+ YLGD  SE KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  IEHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILD 120
            EH+ENV+KV+ RLK R+  KDGLVC+ARKPW+AVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+K++M A+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTY 240
           +DTV AYEI+LA G +VRAT+DN+YSDL+YAIPWSQGTLG LV+AEI+LI +KEYM+LTY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKK 300
            P KG+LQ + Q Y+DSF P+D D+    K+PDFVE M+Y+PTE V M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQW 360
           KGN IN++GWWFKPWFYQHAQ ALKKG+FVEYIPTREYYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MH++QDMLVPL ++GD LEW+ REMEVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 LWLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQ 480
           +WLCPH+LFK P++  + PEPGFE + +QGDT  AQMYTD+GVYYAPG VLRGE +DG++
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKK 540
           AVR ME WLIE HGFQPQ++V+E+ EK FW+MF+ ELYE CRKKY AIGTFM V+YK KK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GKKTEKEVQEAEQAQAETAYADIE 564
           G+KTEKEV+EAEQA  ETAYA+ +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of Tan0016236 vs. ExPASy Swiss-Prot
Match: Q5BQE6 (Delta(24)-sterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr24 PE=2 SV=2)

HSP 1 Score: 375.6 bits (963), Expect = 1.0e-102
Identity = 218/533 (40.90%), Postives = 311/533 (58.35%), Query Frame = 0

Query: 12  RKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQIEHEENVQKVVD 71
           R KG   +L++ RW+ V   +LP S  F    Y+      + S   R   HE+ VQ +  
Sbjct: 19  RVKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRL--HEQRVQDIQK 78

Query: 72  RLKQ-RNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDIDKDRMIAKVE 131
           ++++ +       +C+ R  WL V +R   YK+  H  + ++   +IL++D  + I +VE
Sbjct: 79  QVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVE 138

Query: 132 PHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIIL 191
           P V+MGQ++     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+IL
Sbjct: 139 PLVSMGQVTALLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELIL 198

Query: 192 ADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYKPAKGNLQEIG 251
           ADG  VR T  ++ SDLFYA+PWS GTLGFLV+AEI++I  K+Y+KL ++P +G      
Sbjct: 199 ADGSFVRCT-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL----- 258

Query: 252 QAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGNVINSIGWW 311
           +A  + FT      +N      FVE ++YS  EAV MTG      E  K    +NSIG +
Sbjct: 259 EAICEKFTHESQRLEN-----HFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSY 318

Query: 312 FKPWFYQHAQKALKKG-EFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMM 371
           +KPWF++H +  LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+L GWM+
Sbjct: 319 YKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMV 378

Query: 372 PPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPLWLCPHRLFK 431
           PPK+SLLK TQGE +R  Y + H+VQDMLVP++ +   L     ++ VYP+WLCP  L  
Sbjct: 379 PPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLSQALHTFQNDIHVYPIWLCPFIL-- 438

Query: 432 PPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQAVRNMESWLI 491
           P    +V P         +GD   A++Y DIG Y  P    R + ++    +R +E ++ 
Sbjct: 439 PSQPGLVHP---------KGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVR 498

Query: 492 EIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYK-CKKGK 542
            +HGFQ  ++   M+ +EFW+MFD  LY   RK+ G    F  V+ K CK  +
Sbjct: 499 SVHGFQMLYADCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

BLAST of Tan0016236 vs. ExPASy Swiss-Prot
Match: Q15392 (Delta(24)-sterol reductase OS=Homo sapiens OX=9606 GN=DHCR24 PE=1 SV=2)

HSP 1 Score: 373.6 bits (958), Expect = 3.9e-102
Identity = 217/533 (40.71%), Postives = 311/533 (58.35%), Query Frame = 0

Query: 12  RKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQIEHEENVQKVVD 71
           R KG   +L++ RW+ V   +LP S  F    Y+      + S   R   HE+ V+ +  
Sbjct: 19  RLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRL--HEQRVRDIQK 78

Query: 72  RLKQ-RNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDIDKDRMIAKVE 131
           ++++ +       +C+ R  WL V +R   YK+  H  + ++   +IL++D  + I +VE
Sbjct: 79  QVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVE 138

Query: 132 PHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIIL 191
           P V MGQ++     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE++L
Sbjct: 139 PLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVL 198

Query: 192 ADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYKPAKGNLQEIG 251
           ADG  VR T  ++ SDLFYA+PWS GTLGFLV+AEI++I  K+Y+KL ++P +G      
Sbjct: 199 ADGSFVRCT-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL----- 258

Query: 252 QAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGNVINSIGWW 311
           +A    FT     Q+N      FVE ++YS  EAV MTG    + E  K    +NSIG +
Sbjct: 259 EAICAKFTHESQRQEN-----HFVEGLLYSLDEAVIMTGVMTDEAEPSK----LNSIGNY 318

Query: 312 FKPWFYQHAQKALKKG-EFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMM 371
           +KPWF++H +  LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+L GWM+
Sbjct: 319 YKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMV 378

Query: 372 PPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPLWLCPHRLFK 431
           PPK+SLLK TQGE +R  Y + H+VQDMLVP++ +   L     ++ VYP+WLCP  L  
Sbjct: 379 PPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFIL-- 438

Query: 432 PPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQAVRNMESWLI 491
           P    +V P+ G E          A++Y DIG Y  P    R + ++    +R +E ++ 
Sbjct: 439 PSQPGLVHPK-GNE----------AELYIDIGAYGEP----RVKHFEARSCMRQLEKFVR 498

Query: 492 EIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYK-CKKGK 542
            +HGFQ  ++   M+ +EFW+MFD  LY   R+K G    F  V+ K CK  +
Sbjct: 499 SVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAAR 515

BLAST of Tan0016236 vs. ExPASy Swiss-Prot
Match: Q8VCH6 (Delta(24)-sterol reductase OS=Mus musculus OX=10090 GN=Dhcr24 PE=1 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 3.9e-102
Identity = 217/533 (40.71%), Postives = 311/533 (58.35%), Query Frame = 0

Query: 12  RKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQIEHEENVQKVVD 71
           R KG   +L++ RW+ V   +LP S  F    Y+      + S   R   HE+ V+ +  
Sbjct: 19  RVKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRL--HEQRVRDIQK 78

Query: 72  RLKQ-RNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDIDKDRMIAKVE 131
           ++++ +       +C+ R  WL V +R   YK+  H  + ++   +IL++D  + I +VE
Sbjct: 79  QVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVE 138

Query: 132 PHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIIL 191
           P V+MGQ++     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+IL
Sbjct: 139 PLVSMGQVTALLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELIL 198

Query: 192 ADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYKPAKGNLQEIG 251
           ADG  VR T  ++ SDLFYA+PWS GTLGFLV+AEI++I  K+Y+KL ++P +G      
Sbjct: 199 ADGSFVRCT-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL----- 258

Query: 252 QAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGNVINSIGWW 311
           +A  + FT      +N      FVE ++YS  EAV MTG      E  K    +NSIG +
Sbjct: 259 EAICEKFTRESQRLEN-----HFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSY 318

Query: 312 FKPWFYQHAQKALKKG-EFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMM 371
           +KPWF++H +  LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+L GWM+
Sbjct: 319 YKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMV 378

Query: 372 PPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPLWLCPHRLFK 431
           PPK+SLLK TQGE +R  Y + H+VQDMLVP++ +   L     ++ VYP+WLCP  L  
Sbjct: 379 PPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCMSQALHTFQNDIHVYPIWLCPFIL-- 438

Query: 432 PPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQAVRNMESWLI 491
           P    +V P         +GD   A++Y DIG Y  P    R + ++    +R +E ++ 
Sbjct: 439 PSQPGLVHP---------KGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVR 498

Query: 492 EIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYK-CKKGK 542
            +HGFQ  ++   M+ +EFW+MFD  LY   RK+ G    F  V+ K CK  +
Sbjct: 499 SVHGFQMLYADCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

BLAST of Tan0016236 vs. NCBI nr
Match: XP_038877985.1 (delta(24)-sterol reductase-like [Benincasa hispida] >XP_038877986.1 delta(24)-sterol reductase-like [Benincasa hispida] >XP_038877988.1 delta(24)-sterol reductase-like [Benincasa hispida] >XP_038877989.1 delta(24)-sterol reductase-like [Benincasa hispida])

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 540/567 (95.24%), Postives = 557/567 (98.24%), Query Frame = 0

Query: 1   MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           M+DLEASKPQKRKKGWVD+LVNFRWIIVIFVVLPFSFSFYF+QYLGDKRSERKSYKQRQI
Sbjct: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDI 120
           EHEENVQKVV+RLKQRNP KDGLVC+ARKPWLAVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYK 240
           DTVVAYEI+LADGRVVRATKDN+YSDLFYAIPWSQGTLGFLVSAEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           P KG+LQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK
Sbjct: 241 PVKGDLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTR+YYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPL 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWI REMEVYPL
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420

Query: 421 WLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480
           WLCPHRLFKPPM+TMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA
Sbjct: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480

Query: 481 VRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKG 540
           V NME+WLIEIHGFQPQHSVTEMSEK+FWKMFDPELYE CRKKYGAIGTFMHV+YKCKKG
Sbjct: 481 VHNMENWLIEIHGFQPQHSVTEMSEKDFWKMFDPELYEACRKKYGAIGTFMHVYYKCKKG 540

Query: 541 KKTEKEVQEAEQAQAETAYADIEQPAE 568
           +KTEKEVQEAE+AQAETAY D+EQ AE
Sbjct: 541 RKTEKEVQEAEKAQAETAYVDVEQAAE 567

BLAST of Tan0016236 vs. NCBI nr
Match: XP_022153163.1 (delta(24)-sterol reductase-like [Momordica charantia] >XP_022153164.1 delta(24)-sterol reductase-like [Momordica charantia] >XP_022153165.1 delta(24)-sterol reductase-like [Momordica charantia])

HSP 1 Score: 1125.2 bits (2909), Expect = 0.0e+00
Identity = 538/567 (94.89%), Postives = 555/567 (97.88%), Query Frame = 0

Query: 1   MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSDLEASKPQKRKKGWVD LVNFRWIIV+FVVLPFSF FYF+QYLGDKRSERKSYKQRQI
Sbjct: 1   MSDLEASKPQKRKKGWVDFLVNFRWIIVVFVVLPFSFGFYFVQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDI 120
           EH ENVQKVV+RLKQR+PLKDGLVC+ARKPWLAVGMRNVDYKRARH+EVDLS FRNILDI
Sbjct: 61  EHGENVQKVVERLKQRDPLKDGLVCTARKPWLAVGMRNVDYKRARHYEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYK 240
           DTVVAYEI+LADGRVVRATKDN+YSDLF+ IPWSQGTLGFLVSAEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFFGIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK
Sbjct: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPL 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYH+MHIVQDMLVPLRRIGDTLEWI REMEVYPL
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHDMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420

Query: 421 WLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480
           WLCPHRLFKPPM+TMV+PEPGFELQL QGDT YAQMYTDIGVYYAPGPVLRGEVYDGAQA
Sbjct: 421 WLCPHRLFKPPMKTMVTPEPGFELQLTQGDTTYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480

Query: 481 VRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKG 540
           VRNMESWLIEIHGFQPQHSVTEM+EKEFW+MFDPELYE CRKKYGAIGTFMHV+YKCKKG
Sbjct: 481 VRNMESWLIEIHGFQPQHSVTEMNEKEFWRMFDPELYEACRKKYGAIGTFMHVYYKCKKG 540

Query: 541 KKTEKEVQEAEQAQAETAYADIEQPAE 568
           +KTEKEVQEAEQAQ ETAYADIEQPAE
Sbjct: 541 RKTEKEVQEAEQAQPETAYADIEQPAE 567

BLAST of Tan0016236 vs. NCBI nr
Match: XP_004137177.1 (delta(24)-sterol reductase [Cucumis sativus] >XP_011653373.1 delta(24)-sterol reductase [Cucumis sativus] >XP_031740073.1 delta(24)-sterol reductase [Cucumis sativus] >XP_031740074.1 delta(24)-sterol reductase [Cucumis sativus] >XP_031740075.1 delta(24)-sterol reductase [Cucumis sativus] >KGN53687.1 hypothetical protein Csa_014693 [Cucumis sativus])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 536/567 (94.53%), Postives = 554/567 (97.71%), Query Frame = 0

Query: 1   MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           M+DLEASKPQKRKKGWVD+LVNFRWIIVIFVVLPFSFSFYF+QYLGDKRSERKSYKQRQI
Sbjct: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDI 120
           EHEENVQKVV+RLKQRNP KDGLVC+ARKPWLAVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYK 240
           DTVVAYEI+LADGRVVRATKDN+YSDLFYAIPWSQGTLGFLVSAEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           P KGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK
Sbjct: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN+IGWWFKPWFYQHAQK+LK GEFVEYIPTREYYHRHT+SLYWEGKLILPFADQWW
Sbjct: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPL 420
           FRF LGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWI REMEVYPL
Sbjct: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420

Query: 421 WLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480
           WLCPHRLFKPPM+TMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDG QA
Sbjct: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480

Query: 481 VRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKG 540
           VRNME+WLIEIHGFQPQHSVTEMSEKEFWKMFD ELYE CRKKYGAIGTFMHV+YKCKKG
Sbjct: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540

Query: 541 KKTEKEVQEAEQAQAETAYADIEQPAE 568
           +KTEKEV+EAE+AQAETAYAD+EQ  E
Sbjct: 541 RKTEKEVEEAEKAQAETAYADVEQAGE 567

BLAST of Tan0016236 vs. NCBI nr
Match: KAG6606910.1 (Delta(24)-sterol reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 535/567 (94.36%), Postives = 557/567 (98.24%), Query Frame = 0

Query: 1   MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD+EASKPQKRKKGWVD LVNFRWI+VIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI
Sbjct: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDI 120
           EHEENVQKVV+RLK+RNP KDGLVC+ARKPWLAVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHEENVQKVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYK 240
           DTVVAYEI+LADGRVVRATKDN+YSDLFYAIPWSQGTLGFLVSAEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYAS+EEAKKK
Sbjct: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASREEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPL 420
           FRFL+GWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWI +EMEVYPL
Sbjct: 361 FRFLMGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420

Query: 421 WLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480
           WLCPHRLFKPPM+TMV+PEPGFELQLKQGDTPYAQMYTDIGVYYAPG VLRGEVYDG +A
Sbjct: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480

Query: 481 VRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKG 540
           V+NM +WLIEIHGFQPQHSVTEM+E+EFWKMF+PELYEVCRKKYGAIGTFMHV+YKCKKG
Sbjct: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540

Query: 541 KKTEKEVQEAEQAQAETAYADIEQPAE 568
           +KTEKEVQEAE+AQ ETAYADIEQPAE
Sbjct: 541 RKTEKEVQEAERAQPETAYADIEQPAE 567

BLAST of Tan0016236 vs. NCBI nr
Match: XP_023523527.1 (delta(24)-sterol reductase-like [Cucurbita pepo subsp. pepo] >XP_023523528.1 delta(24)-sterol reductase-like [Cucurbita pepo subsp. pepo] >XP_023523529.1 delta(24)-sterol reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 534/567 (94.18%), Postives = 556/567 (98.06%), Query Frame = 0

Query: 1   MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD+EASKPQKRKKGWVD LVNFRWI+VIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI
Sbjct: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDI 120
           EHEENVQKVV+RLK+RNP KDGLVC+ARKPWLAVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHEENVQKVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYK 240
           DTVVAYEI+LADGRVVRATKDN+YSDLFYAIPWSQGTLGFLVSAEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYAS EEAKKK
Sbjct: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPL 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWI +EMEVYPL
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420

Query: 421 WLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480
           WLCPHRLFKPP++TMV+PEPGFELQLKQGDTPYAQMYTDIGVYYAPG VLRGEVYDG +A
Sbjct: 421 WLCPHRLFKPPIKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480

Query: 481 VRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKG 540
           V+NM +WLIEIHGFQPQHSVTEM+E+EFWKMF+PELYEVCRKKYGAIGTFMHV+YKCKKG
Sbjct: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540

Query: 541 KKTEKEVQEAEQAQAETAYADIEQPAE 568
           +KTEKEVQEAE+AQ ETAYAD+EQPAE
Sbjct: 541 RKTEKEVQEAERAQPETAYADLEQPAE 567

BLAST of Tan0016236 vs. ExPASy TrEMBL
Match: A0A6J1DI89 (Delta(24)-sterol reductase OS=Momordica charantia OX=3673 GN=LOC111020726 PE=4 SV=1)

HSP 1 Score: 1125.2 bits (2909), Expect = 0.0e+00
Identity = 538/567 (94.89%), Postives = 555/567 (97.88%), Query Frame = 0

Query: 1   MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSDLEASKPQKRKKGWVD LVNFRWIIV+FVVLPFSF FYF+QYLGDKRSERKSYKQRQI
Sbjct: 1   MSDLEASKPQKRKKGWVDFLVNFRWIIVVFVVLPFSFGFYFVQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDI 120
           EH ENVQKVV+RLKQR+PLKDGLVC+ARKPWLAVGMRNVDYKRARH+EVDLS FRNILDI
Sbjct: 61  EHGENVQKVVERLKQRDPLKDGLVCTARKPWLAVGMRNVDYKRARHYEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYK 240
           DTVVAYEI+LADGRVVRATKDN+YSDLF+ IPWSQGTLGFLVSAEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFFGIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK
Sbjct: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPL 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYH+MHIVQDMLVPLRRIGDTLEWI REMEVYPL
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHDMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420

Query: 421 WLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480
           WLCPHRLFKPPM+TMV+PEPGFELQL QGDT YAQMYTDIGVYYAPGPVLRGEVYDGAQA
Sbjct: 421 WLCPHRLFKPPMKTMVTPEPGFELQLTQGDTTYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480

Query: 481 VRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKG 540
           VRNMESWLIEIHGFQPQHSVTEM+EKEFW+MFDPELYE CRKKYGAIGTFMHV+YKCKKG
Sbjct: 481 VRNMESWLIEIHGFQPQHSVTEMNEKEFWRMFDPELYEACRKKYGAIGTFMHVYYKCKKG 540

Query: 541 KKTEKEVQEAEQAQAETAYADIEQPAE 568
           +KTEKEVQEAEQAQ ETAYADIEQPAE
Sbjct: 541 RKTEKEVQEAEQAQPETAYADIEQPAE 567

BLAST of Tan0016236 vs. ExPASy TrEMBL
Match: A0A0A0KZ82 (Delta(24)-sterol reductase OS=Cucumis sativus OX=3659 GN=Csa_4G103350 PE=4 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 536/567 (94.53%), Postives = 554/567 (97.71%), Query Frame = 0

Query: 1   MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           M+DLEASKPQKRKKGWVD+LVNFRWIIVIFVVLPFSFSFYF+QYLGDKRSERKSYKQRQI
Sbjct: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDI 120
           EHEENVQKVV+RLKQRNP KDGLVC+ARKPWLAVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYK 240
           DTVVAYEI+LADGRVVRATKDN+YSDLFYAIPWSQGTLGFLVSAEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           P KGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK
Sbjct: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN+IGWWFKPWFYQHAQK+LK GEFVEYIPTREYYHRHT+SLYWEGKLILPFADQWW
Sbjct: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPL 420
           FRF LGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWI REMEVYPL
Sbjct: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420

Query: 421 WLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480
           WLCPHRLFKPPM+TMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDG QA
Sbjct: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480

Query: 481 VRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKG 540
           VRNME+WLIEIHGFQPQHSVTEMSEKEFWKMFD ELYE CRKKYGAIGTFMHV+YKCKKG
Sbjct: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540

Query: 541 KKTEKEVQEAEQAQAETAYADIEQPAE 568
           +KTEKEV+EAE+AQAETAYAD+EQ  E
Sbjct: 541 RKTEKEVEEAEKAQAETAYADVEQAGE 567

BLAST of Tan0016236 vs. ExPASy TrEMBL
Match: A0A6J1G9H4 (Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111452014 PE=4 SV=1)

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 534/566 (94.35%), Postives = 555/566 (98.06%), Query Frame = 0

Query: 1   MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD+EASKPQKRKKGWVD LVNFRWI+VIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI
Sbjct: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDI 120
           EHEENVQKVV+RLK+RNP KDGLVC+ARKPWLAVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHEENVQKVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYK 240
           DTVVAYEI+LADGRVVRATKDN+YSDLFYAIPWSQGTLGFLVSAEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYAS EEAKKK
Sbjct: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPL 420
           FRFL+GWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWI +EMEVYPL
Sbjct: 361 FRFLMGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420

Query: 421 WLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480
           WLCPHRLFKPPM+TMV+PEPGFELQLKQGDTPYAQMYTDIGVYYAPG VLRGEVYDG +A
Sbjct: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480

Query: 481 VRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKG 540
           V+NM +WLIEIHGFQPQHSVTEM+E+EFWKMF+PELYEVCRKKYGAIGTFMHV+YKCKKG
Sbjct: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540

Query: 541 KKTEKEVQEAEQAQAETAYADIEQPA 567
           +KTEKEVQEAE+AQ ETAYADIEQPA
Sbjct: 541 RKTEKEVQEAERAQPETAYADIEQPA 566

BLAST of Tan0016236 vs. ExPASy TrEMBL
Match: A0A6J1KA67 (Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111493057 PE=4 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 535/567 (94.36%), Postives = 555/567 (97.88%), Query Frame = 0

Query: 1   MSDLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD+EASKPQKRKKGWVD LVNFRWI+VIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI
Sbjct: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDI 120
           EHEENVQ VV+RLK+RNP KDGLVC+ARKPWLAVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYK 240
           DTVVAYEI+LADGRVVRATKDN+YSDLFYAIPWSQGTLGFLVSAEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYAS EEAKKK
Sbjct: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPL 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWI +EMEVYPL
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420

Query: 421 WLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQA 480
           WLCPHRLFKPPM+TMV+PEPGFELQLKQGDTPYAQMYTDIGVYYAPG VLRGEVYDG +A
Sbjct: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480

Query: 481 VRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKG 540
           V+NM +WLIEIHGFQPQHSVTEM+E+EFWKMF+PELYEVCRKKYGAIGTFMHV+YKCKKG
Sbjct: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540

Query: 541 KKTEKEVQEAEQAQAETAYADIEQPAE 568
           +KTEKEVQEAE+AQ ETAYADIEQPAE
Sbjct: 541 RKTEKEVQEAERAQPETAYADIEQPAE 567

BLAST of Tan0016236 vs. ExPASy TrEMBL
Match: A0A1S3CCJ1 (Delta(24)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC103499124 PE=4 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 536/565 (94.87%), Postives = 552/565 (97.70%), Query Frame = 0

Query: 3   DLEASKPQKRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQIEH 62
           DLEASKPQKRKKGWVD+LVNFRWIIVIFVVLPFSFSFYF+QYLGDKRSERKSYKQRQIEH
Sbjct: 2   DLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQIEH 61

Query: 63  EENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILDIDK 122
           EENVQKVV+RLKQRNP KDGLVC+ARKPWLAVGMRNVDYKRARHFEVDLS FRNILDIDK
Sbjct: 62  EENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDK 121

Query: 123 DRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDT 182
           +RMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDT
Sbjct: 122 ERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDT 181

Query: 183 VVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYKPA 242
           VVAYEI+LADGRVVRATKDN+YSDLFYAIPWSQGTLGFLVSAEIKLI IKEYMKLTY P 
Sbjct: 182 VVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYLPV 241

Query: 243 KGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGN 302
           KG LQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGN
Sbjct: 242 KGKLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGN 301

Query: 303 VINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFR 362
           VINSIGWWFKPWFYQHAQKAL KGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFR
Sbjct: 302 VINSIGWWFKPWFYQHAQKALIKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFR 361

Query: 363 FLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYPLWL 422
           FLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWI REMEVYPLWL
Sbjct: 362 FLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPLWL 421

Query: 423 CPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQAVR 482
           CPHRLFKPPM+TMVSPEPGFELQ+KQGDT YAQMYTDIGVYYAPGPVLRGEVYDGAQAVR
Sbjct: 422 CPHRLFKPPMKTMVSPEPGFELQIKQGDTSYAQMYTDIGVYYAPGPVLRGEVYDGAQAVR 481

Query: 483 NMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKKGKK 542
           NME+WLIEIHGFQPQHSVTEMSEKEFWKMFD ELYE CRKKYGAIGTFMHV+YKCKKG+K
Sbjct: 482 NMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKGRK 541

Query: 543 TEKEVQEAEQAQAETAYADIEQPAE 568
           TEKE++EAE AQAETAYAD+EQ +E
Sbjct: 542 TEKEIEEAENAQAETAYADVEQASE 566

BLAST of Tan0016236 vs. TAIR 10
Match: AT3G19820.1 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 891.3 bits (2302), Expect = 3.9e-259
Identity = 418/564 (74.11%), Postives = 490/564 (86.88%), Query Frame = 0

Query: 1   MSDLEASKPQ-KRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQ 60
           MSDL+    + KRKK WVD  V FRWIIVIF+VLPFS +FYF+ YLGD  SE KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  IEHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILD 120
            EH+ENV+KV+ RLK R+  KDGLVC+ARKPW+AVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+K++M A+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTY 240
           +DTV AYEI+LA G +VRAT+DN+YSDL+YAIPWSQGTLG LV+AEI+LI +KEYM+LTY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKK 300
            P KG+LQ + Q Y+DSF P+D D+    K+PDFVE M+Y+PTE V M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQW 360
           KGN IN++GWWFKPWFYQHAQ ALKKG+FVEYIPTREYYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MH++QDMLVPL ++GD LEW+ REMEVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 LWLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQ 480
           +WLCPH+LFK P++  + PEPGFE + +QGDT  AQMYTD+GVYYAPG VLRGE +DG++
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKK 540
           AVR ME WLIE HGFQPQ++V+E+ EK FW+MF+ ELYE CRKKY AIGTFM V+YK KK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GKKTEKEVQEAEQAQAETAYADIE 564
           G+KTEKEV+EAEQA  ETAYA+ +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of Tan0016236 vs. TAIR 10
Match: AT3G19820.2 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 891.3 bits (2302), Expect = 3.9e-259
Identity = 418/564 (74.11%), Postives = 490/564 (86.88%), Query Frame = 0

Query: 1   MSDLEASKPQ-KRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQ 60
           MSDL+    + KRKK WVD  V FRWIIVIF+VLPFS +FYF+ YLGD  SE KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  IEHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILD 120
            EH+ENV+KV+ RLK R+  KDGLVC+ARKPW+AVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+K++M A+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTY 240
           +DTV AYEI+LA G +VRAT+DN+YSDL+YAIPWSQGTLG LV+AEI+LI +KEYM+LTY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKK 300
            P KG+LQ + Q Y+DSF P+D D+    K+PDFVE M+Y+PTE V M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQW 360
           KGN IN++GWWFKPWFYQHAQ ALKKG+FVEYIPTREYYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MH++QDMLVPL ++GD LEW+ REMEVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 LWLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQ 480
           +WLCPH+LFK P++  + PEPGFE + +QGDT  AQMYTD+GVYYAPG VLRGE +DG++
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKK 540
           AVR ME WLIE HGFQPQ++V+E+ EK FW+MF+ ELYE CRKKY AIGTFM V+YK KK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GKKTEKEVQEAEQAQAETAYADIE 564
           G+KTEKEV+EAEQA  ETAYA+ +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of Tan0016236 vs. TAIR 10
Match: AT3G19820.3 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 891.3 bits (2302), Expect = 3.9e-259
Identity = 418/564 (74.11%), Postives = 490/564 (86.88%), Query Frame = 0

Query: 1   MSDLEASKPQ-KRKKGWVDLLVNFRWIIVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQ 60
           MSDL+    + KRKK WVD  V FRWIIVIF+VLPFS +FYF+ YLGD  SE KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  IEHEENVQKVVDRLKQRNPLKDGLVCSARKPWLAVGMRNVDYKRARHFEVDLSPFRNILD 120
            EH+ENV+KV+ RLK R+  KDGLVC+ARKPW+AVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+K++M A+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIILADGRVVRATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTY 240
           +DTV AYEI+LA G +VRAT+DN+YSDL+YAIPWSQGTLG LV+AEI+LI +KEYM+LTY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKK 300
            P KG+LQ + Q Y+DSF P+D D+    K+PDFVE M+Y+PTE V M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQW 360
           KGN IN++GWWFKPWFYQHAQ ALKKG+FVEYIPTREYYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIDREMEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MH++QDMLVPL ++GD LEW+ REMEVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 LWLCPHRLFKPPMRTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGAQ 480
           +WLCPH+LFK P++  + PEPGFE + +QGDT  AQMYTD+GVYYAPG VLRGE +DG++
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRNMESWLIEIHGFQPQHSVTEMSEKEFWKMFDPELYEVCRKKYGAIGTFMHVFYKCKK 540
           AVR ME WLIE HGFQPQ++V+E+ EK FW+MF+ ELYE CRKKY AIGTFM V+YK KK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GKKTEKEVQEAEQAQAETAYADIE 564
           G+KTEKEV+EAEQA  ETAYA+ +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of Tan0016236 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 48.5 bits (114), Expect = 2.0e-05
Identity = 28/92 (30.43%), Postives = 46/92 (50.00%), Query Frame = 0

Query: 137 QISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIILADGRVV 196
           ++   T+ + L+     +   LTVGG ++  GI G +  YG     V+  ++I   G + 
Sbjct: 150 EVLNKTLELGLTPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIA 209

Query: 197 RATKDNDYSDLFYAIPWSQGTLGFLVSAEIKL 229
             +KD + SDLF+A+    G  G +  A IKL
Sbjct: 210 TCSKDMN-SDLFFAVLGGLGQFGIITRARIKL 240

BLAST of Tan0016236 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 47.4 bits (111), Expect = 4.5e-05
Identity = 41/144 (28.47%), Postives = 65/144 (45.14%), Query Frame = 0

Query: 138 ISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIILADGRVVR 197
           I R T+   LS     +   LTVGG ++  GI G +  +G   + V   EI+   G VV 
Sbjct: 170 ILRETLKYGLSPKSWTDYLHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVT 229

Query: 198 ATKDNDYSDLFYAIPWSQGTLGFLVSAEIKLIDIKEYMKLTYKPAKGNLQEIGQAYMDSF 257
            ++  + S+LF+++    G  G +  A I L           +PA   ++ I   Y D F
Sbjct: 230 CSEKRN-SELFFSVLGGLGQFGIITRARISL-----------EPAPHMVKWIRVLYSD-F 289

Query: 258 TPRDLDQD---NPEKVPDFVETMI 279
           +    DQ+   + EK  D+VE  +
Sbjct: 290 SAFSRDQEYLISKEKTFDYVEGFV 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P934721.6e-27679.01Delta(24)-sterol reductase OS=Pisum sativum OX=3888 GN=DIM PE=1 SV=1[more]
Q390855.5e-25874.11Delta(24)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=DIM PE=1 SV=2[more]
Q5BQE61.0e-10240.90Delta(24)-sterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr24 PE=2 SV=2[more]
Q153923.9e-10240.71Delta(24)-sterol reductase OS=Homo sapiens OX=9606 GN=DHCR24 PE=1 SV=2[more]
Q8VCH63.9e-10240.71Delta(24)-sterol reductase OS=Mus musculus OX=10090 GN=Dhcr24 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038877985.10.0e+0095.24delta(24)-sterol reductase-like [Benincasa hispida] >XP_038877986.1 delta(24)-st... [more]
XP_022153163.10.0e+0094.89delta(24)-sterol reductase-like [Momordica charantia] >XP_022153164.1 delta(24)-... [more]
XP_004137177.10.0e+0094.53delta(24)-sterol reductase [Cucumis sativus] >XP_011653373.1 delta(24)-sterol re... [more]
KAG6606910.10.0e+0094.36Delta(24)-sterol reductase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023523527.10.0e+0094.18delta(24)-sterol reductase-like [Cucurbita pepo subsp. pepo] >XP_023523528.1 del... [more]
Match NameE-valueIdentityDescription
A0A6J1DI890.0e+0094.89Delta(24)-sterol reductase OS=Momordica charantia OX=3673 GN=LOC111020726 PE=4 S... [more]
A0A0A0KZ820.0e+0094.53Delta(24)-sterol reductase OS=Cucumis sativus OX=3659 GN=Csa_4G103350 PE=4 SV=1[more]
A0A6J1G9H40.0e+0094.35Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111452014 PE=4 SV... [more]
A0A6J1KA670.0e+0094.36Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111493057 PE=4 SV=1[more]
A0A1S3CCJ10.0e+0094.87Delta(24)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC103499124 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G19820.13.9e-25974.11cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT3G19820.23.9e-25974.11cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT3G19820.33.9e-25974.11cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT5G56970.12.0e-0530.43cytokinin oxidase 3 [more]
AT2G41510.14.5e-0528.47cytokinin oxidase/dehydrogenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 107..198
e-value: 2.5E-14
score: 53.2
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 113..231
e-value: 1.5E-25
score: 91.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 548..567
NoneNo IPR availablePANTHERPTHR10801:SF16S-LOCUS GLYCOPROTEIN DOMAIN, BULB-TYPE LECTIN DOMAIN, FAD-BINDING, TYPE 2-RELATEDcoord: 1..564
IPR040165Delta(24)-sterol reductasePANTHERPTHR1080124-DEHYDROCHOLESTEROL REDUCTASEcoord: 1..564
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 48..231
score: 13.004426
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 104..230

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0016236.1Tan0016236.1mRNA
Tan0016236.2Tan0016236.2mRNA
Tan0016236.3Tan0016236.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008202 steroid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050614 delta24-sterol reductase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity