Tan0016025 (gene) Snake gourd v1

Overview


Name	Tan0016025
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	GDSL esterase/lipase
Location	LG07: 9541244 .. 9542910 (-)
RNA-Seq Expression	Tan0016025
Synteny	Tan0016025

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCGTAATAGGGTTTGCATTTGTGGCGATGGAGTTCAGAATCGCAGCCATTTTCAAGCCTTTCTTCTTCACTGTTCATCGGATCTTCTTGATTGCTCTGTTATTGAGGATGGGCCTCGATAATATTCTTGCTGCGGCTGCGGCTGCAAATGAAAGATCGAATAATAATGTGACTGCCATGTTCTTGTTGGGAGATTCTTCAGTGGATTGCGGCTTAAACACTCTGTTTTATCCTCTTCTCCATCGGAATTTCTCCCTCCTTCCTTGCGATTCCAATCAAACTACTCTTCTTCCTCATCTTCTTGGTTTGTGCTTTCTCTCTGCTATTCATTTTTTCCATTTCTCTTTTAAACTTGTTTCTATCACTTTCTTCTTCTTCTTCTTCTTCTACTTCTATATTTATATTTGCCTTTCTTTCCATTTCACTCGAAAGCGTTGTATTTACGCTTGTATTATTGTTTATGTGAACATATTTGGACATATACGTTGAATAAAAAATCAAAACATATGCAAATACGTTATTTGTCATCCATAACGAAATTATTTTGAAACAAATTTCAAAGTTCAAAGGTTAAAATAAAAAATTTTAAAGTTCAAAGATATAATTGAAACTAATCTCATAATTCATGAATATTTTTTGATAATTTAATCTTTCCAAAAATAGCCCTATTCTTTCTAAATTTGCAATATTACCTTTTATTTTTCATAAACATTTCAAAACATCTATTAGAATAGAAAAACTGTTAAATATTTGAGAAAAAATTGATCAATTTGTATGTCGTCATTCTTTCATATTTAACGTCTTGGTCACTATAACGTCATTAAAAAAAAAAAAGAAGATAAAATTTAAGGGTATTTTTTTAATATATAATTTAGCATAATATAATCTATATATTCCTGCATTTAATGAAAAGCCAGGGTATTTTTGCAGCTGAGAAGATAGGCGTACCACAATCTCAACCCTTGTACATTCAAAATGGTTCCATTGAAGCAATCCTAAATGGCCTCAGTTTTGGTTCGCCACAGGCAACAATCATGAGCAATGCCCAAAGCTATCAGTCTCTCAACCAACAACTACGCCAAGTACTCGACACCATTCAGCTCTTGCGCCTGCGACTCGGGCTAGACACTGCTCGTCATTTCATCCAATCTTCCATTTTCTACCTCTCATTTGGCGAGGTCGATTTCATCAATCTCTACCTTCTAAACTCATCAACGACGAGCAAAAACTCAAAGTATGAGGGCGAGGAATTCACTAGGTTACTAGTATCACAAATGTTGGTTGCTATACGAAACCTCCAGGAAGCAGGTGCGAGGAAGATCATATGCATGGGAATACTCCCATTGGGATGCACTCCTCGAGTGCTATCTCTATGGCGCGATTCCCCTACGGCGATATACGACAAGAATGGATGCGTAAAAGAGATGAATGAGTTGGTTGCGAAGTACAACGACCTAATGGAGAAGCAGATCGTGAAGCTGAACGAACAATATGGTGATACTCGAATGATTTTTTGCGACGCGTATAAAGGGATGATGGAGATCATTGGCAACCCGAGACACTACGGTATGTTGTTGTTAGTAAGGGTATATAAATAACTCCGTAACTCGGACTACCCAACCCAAAGCGTGAGAGTTGGGTTGTGTTAGGTTTTTTCTCGGTTTGGG

mRNA sequence

ATCGTAATAGGGTTTGCATTTGTGGCGATGGAGTTCAGAATCGCAGCCATTTTCAAGCCTTTCTTCTTCACTGTTCATCGGATCTTCTTGATTGCTCTGTTATTGAGGATGGGCCTCGATAATATTCTTGCTGCGGCTGCGGCTGCAAATGAAAGATCGAATAATAATGTGACTGCCATGTTCTTGTTGGGAGATTCTTCAGTGGATTGCGGCTTAAACACTCTGTTTTATCCTCTTCTCCATCGGAATTTCTCCCTCCTTCCTTGCGATTCCAATCAAACTACTCTTCTTCCTCATCTTCTTGCTGAGAAGATAGGCGTACCACAATCTCAACCCTTGTACATTCAAAATGGTTCCATTGAAGCAATCCTAAATGGCCTCAGTTTTGGTTCGCCACAGGCAACAATCATGAGCAATGCCCAAAGCTATCAGTCTCTCAACCAACAACTACGCCAAGTACTCGACACCATTCAGCTCTTGCGCCTGCGACTCGGGCTAGACACTGCTCGTCATTTCATCCAATCTTCCATTTTCTACCTCTCATTTGGCGAGGTCGATTTCATCAATCTCTACCTTCTAAACTCATCAACGACGAGCAAAAACTCAAAGTATGAGGGCGAGGAATTCACTAGGTTACTAGTATCACAAATGTTGGTTGCTATACGAAACCTCCAGGAAGCAGGTGCGAGGAAGATCATATGCATGGGAATACTCCCATTGGGATGCACTCCTCGAGTGCTATCTCTATGGCGCGATTCCCCTACGGCGATATACGACAAGAATGGATGCGTAAAAGAGATGAATGAGTTGGTTGCGAAGTACAACGACCTAATGGAGAAGCAGATCGTGAAGCTGAACGAACAATATGGTGATACTCGAATGATTTTTTGCGACGCGTATAAAGGGATGATGGAGATCATTGGCAACCCGAGACACTACGGTATGTTGTTGTTAGTAAGGGTATATAAATAACTCCGTAACTCGGACTACCCAACCCAAAGCGTGAGAGTTGGGTTGTGTTAGGTTTTTTCTCGGTTTGGG

Coding sequence (CDS)

ATGGAGTTCAGAATCGCAGCCATTTTCAAGCCTTTCTTCTTCACTGTTCATCGGATCTTCTTGATTGCTCTGTTATTGAGGATGGGCCTCGATAATATTCTTGCTGCGGCTGCGGCTGCAAATGAAAGATCGAATAATAATGTGACTGCCATGTTCTTGTTGGGAGATTCTTCAGTGGATTGCGGCTTAAACACTCTGTTTTATCCTCTTCTCCATCGGAATTTCTCCCTCCTTCCTTGCGATTCCAATCAAACTACTCTTCTTCCTCATCTTCTTGCTGAGAAGATAGGCGTACCACAATCTCAACCCTTGTACATTCAAAATGGTTCCATTGAAGCAATCCTAAATGGCCTCAGTTTTGGTTCGCCACAGGCAACAATCATGAGCAATGCCCAAAGCTATCAGTCTCTCAACCAACAACTACGCCAAGTACTCGACACCATTCAGCTCTTGCGCCTGCGACTCGGGCTAGACACTGCTCGTCATTTCATCCAATCTTCCATTTTCTACCTCTCATTTGGCGAGGTCGATTTCATCAATCTCTACCTTCTAAACTCATCAACGACGAGCAAAAACTCAAAGTATGAGGGCGAGGAATTCACTAGGTTACTAGTATCACAAATGTTGGTTGCTATACGAAACCTCCAGGAAGCAGGTGCGAGGAAGATCATATGCATGGGAATACTCCCATTGGGATGCACTCCTCGAGTGCTATCTCTATGGCGCGATTCCCCTACGGCGATATACGACAAGAATGGATGCGTAAAAGAGATGAATGAGTTGGTTGCGAAGTACAACGACCTAATGGAGAAGCAGATCGTGAAGCTGAACGAACAATATGGTGATACTCGAATGATTTTTTGCGACGCGTATAAAGGGATGATGGAGATCATTGGCAACCCGAGACACTACGGTATGTTGTTGTTAGTAAGGGTATATAAATAA

Protein sequence

MEFRIAAIFKPFFFTVHRIFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVDCGLNTLFYPLLHRNFSLLPCDSNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAILNGLSFGSPQATIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFINLYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTPRVLSLWRDSPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGNPRHYGMLLLVRVYK

Homology

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BLAST of Tan0016025 vs. ExPASy Swiss-Prot
Match: Q9FHQ1 (GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 6.4e-22
Identity = 76/299 (25.42%), Postives = 144/299 (48.16%), Query Frame = 0


Query: 24  LLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVDCGLNT-LFYPLLHRNFSLLPCD- 83
           ++LR+ L  +++  A A   S +++   ++ GDS  + G N  L Y L   +F     D 
Sbjct: 2   MILRLALAIVISTYATAQPASTSSLVT-YIFGDSLTEVGNNNFLQYSLARADFPYYGVDF 61

Query: 84  ---------SNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAILNGLSFGSPQATIMSNAQ 143
                    +N  T +  +++ K+G+    P    + + +A L+G+++ S  A I++   
Sbjct: 62  SGGKATGRFTNGRT-IGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETG 121

Query: 144 SY----QSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFINLYLLNSST 203
            Y     + N Q+     T +++R ++G   A   +  +++++  G  D++N +L     
Sbjct: 122 IYFIQRLTFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFL--QPF 181

Query: 204 TSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTP--RVLSLWRDSPT 263
            +   +Y  +EF  LL S +   +  + + GARK+I  G+ PLGC P  RV S  R    
Sbjct: 182 MADGQQYTHDEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTR---- 241

Query: 264 AIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGNPRHYG 306
                  C+  +NE V ++N   +K ++ LN++    +  F D Y  ++++I NP HYG
Sbjct: 242 ------MCLNRVNEWVLEFNSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYG 286

[Close]

BLAST of Tan0016025 vs. ExPASy Swiss-Prot
Match: Q9FJ41 (GDSL esterase/lipase At5g45950 OS=Arabidopsis thaliana OX=3702 GN=At5g45950 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.6e-17
Identity = 78/280 (27.86%), Postives = 132/280 (47.14%), Query Frame = 0


Query: 44  SNNNVTAMFLLGDSSVDCGLNTLFYPLLHRNFSLLPCDSN-----------QTTLLPHLL 103
           + +NVT++ + GDSSVD G N      +  NF   P   N              L P  +
Sbjct: 34  AKHNVTSILVFGDSSVDPGNNNFIKTEMKGNFP--PYGENFINHKPTGRLCDGLLAPDYI 93

Query: 104 AEKIGVPQSQPLYIQNGSIEA-ILNGLSF---GSPQATIMSNAQSYQSLNQQLRQVLD-T 163
           AE +G P   P ++     +A +  G SF   GS    + +N  +  S   Q    L   
Sbjct: 94  AEAMGYPPI-PAFLDPSLTQADLTRGASFASAGSGYDDLTANISNVWSFTTQANYFLHYK 153

Query: 164 IQLLRLRLGLDTARHFIQSSIFYLSFGEVDFINLYLLNSSTTSKNSKYEGEEFTRLLVSQ 223
           I L +L   L++A+  I ++IF +S G  DF+  YL++    ++  ++  E++   L  +
Sbjct: 154 IHLTKLVGPLESAK-MINNAIFLMSMGSNDFLQNYLVD---FTRQKQFTVEQYIEFLSHR 213

Query: 224 MLVAIRNLQEAGARKIICMGILPLGCTPRVLSLWRDSPTAIYDKNGCVKEMNELVAKYND 283
           ML   + L   GA++++ +G+ P+GC P +  L R   T       CV ++N++   +N 
Sbjct: 214 MLYDAKMLHRLGAKRLVVVGVPPMGCMPLIKYL-RGQKT-------CVDQLNQIAFSFNA 273

Query: 284 LMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGNPRHYGML 308
            + K +  L  + G  + I+ DAY  + E I NPR +G +
Sbjct: 274 KIIKNLELLQSKIG-LKTIYVDAYSTIQEAIKNPRKFGFV 297

[Close]

BLAST of Tan0016025 vs. ExPASy Swiss-Prot
Match: Q8LFJ9 (GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.6e-17
Identity = 76/274 (27.74%), Postives = 128/274 (46.72%), Query Frame = 0


Query: 50  AMFLLGDSSVDCGLNTLFYPLLHRNFSLLPCD--------SNQTTLLPHLLAEKIGVPQS 109
           A F+ GDS VD G N     L   N+     D         N  T++ +  A  +G+P  
Sbjct: 30  AFFVFGDSLVDSGNNNYIPTLARANYFPYGIDFGFPTGRFCNGRTVVDY-GATYLGLPLV 89

Query: 110 QPLYIQNGSI-EAILNGLSFGSPQATIMSNAQSY----QSLNQQLRQVLDTIQLLRLRLG 169
            P Y+   SI +  L G+++ S  A I+     +     + N Q+ Q   TI+L   R  
Sbjct: 90  PP-YLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFF 149

Query: 170 LDTA--RHFIQSSIFYLSFGEVDFINLYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRN 229
            + A  R ++  SI  ++ G  D+IN YL+     S +  Y GE++  LL+  +   I  
Sbjct: 150 QNPADLRKYLAKSIIGINIGSNDYINNYLM-PERYSTSQTYSGEDYADLLIKTLSAQISR 209

Query: 230 LQEAGARKIICMGILPLGCTPRVLSLWRDSPTAIYDKNGCVKEMNELVAKYNDLMEKQIV 289
           L   GARK++  G  PLGC P  LS+   + T     +GCV ++N +V+ +N  ++    
Sbjct: 210 LYNLGARKMVLAGSGPLGCIPSQLSMVTGNNT-----SGCVTKINNMVSMFNSRLKDLAN 269

Query: 290 KLNEQYGDTRMIFCDAYKGMMEIIGNPRHYGMLL 309
            LN     +  ++ + +    +++ NP  YG+++
Sbjct: 270 TLNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVV 295

[Close]

BLAST of Tan0016025 vs. ExPASy Swiss-Prot
Match: Q9FJ40 (GDSL esterase/lipase At5g45960 OS=Arabidopsis thaliana OX=3702 GN=At5g45960 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.8e-16
Identity = 72/302 (23.84%), Postives = 140/302 (46.36%), Query Frame = 0


Query: 19  IFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVDCGLNTLFYPLLHRNFSLL 78
           +FL    +           A +  +  ++V+A+ + GDS+VD G N     +   NF   
Sbjct: 16  LFLFLFFISFSSSTSKLEPAKSEPKRKHSVSAILVFGDSTVDPGNNNYIDTVFKCNFPPY 75

Query: 79  PCDSNQTT---------LLPHLLAEKIGVPQSQPLYIQ-NGSIEAILNGLSF---GSPQA 138
             D    T         L+   +A  IGV ++ P Y+  N  I  +++G+SF   GS   
Sbjct: 76  GLDFRNKTPTGRFCNGRLVTDFIASYIGVKENVPPYLDPNLGINELISGVSFASAGSGYD 135

Query: 139 TIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFINLYLLN 198
            +     +   +  QL    +  + L  ++G       I+ ++F +S G  DF+  Y   
Sbjct: 136 PLTPTITNVIDIPTQLEYFREYKRKLEGKMGKQEMEKHIEEAMFCVSAGTNDFVINYF-- 195

Query: 199 SSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTPRVLSLWRDSP 258
            +   +   +  E + + ++S +   I+ L + GARKI   G+ P+GC P V++L+  S 
Sbjct: 196 -TIPIRRKTFTIEAYQQFVISNLKQFIQGLWKEGARKITVAGLPPIGCLPIVITLF--SG 255

Query: 259 TAIYDKNGCVKEMNELVAKYNDLMEKQ--IVKLNEQYGDTRMIFCDAYKGMMEIIGNPRH 306
            A+ ++  C+   + +   YN L++KQ  ++++   +  +++ + D Y  + E+I +PR 
Sbjct: 256 EALTNRR-CIDRFSTVATNYNFLLQKQLALMQVGLAHLGSKIFYLDVYNPVYEVIRDPRK 311

[Close]

BLAST of Tan0016025 vs. ExPASy Swiss-Prot
Match: Q9SIZ6 (GDSL esterase/lipase At2g40250 OS=Arabidopsis thaliana OX=3702 GN=At2g40250 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 4.0e-16
Identity = 79/312 (25.32%), Postives = 150/312 (48.08%), Query Frame = 0


Query: 10  KPFFFTVHRIFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVDCGLN----T 69
           KP F T    FLI +LL       L      N   +  +TA++  GDS+VD G N    T
Sbjct: 7   KPMFVT----FLINILL-------LQLLNLTNASPSPPITALYAFGDSTVDSGNNNYIPT 66

Query: 70  LF---YPLLHRNF--SLLPCDSNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAI--LNGL 129
           LF   +P   ++F   L     +   L    +   +G+  + P Y+ N S++ +  L G+
Sbjct: 67  LFQSNHPPYGKSFPSKLSTGRFSDGKLATDFIVSSLGLKPTLPAYL-NPSVKPVDLLTGV 126

Query: 130 SFGSPQATI---MSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGE 189
           SF S    +    + +    ++++Q     + +  ++  +G       I++++F +S G 
Sbjct: 127 SFASAGGGLDDRTAKSSLTITMDKQWSYFEEALGKMKSLVGDSETNRVIKNAVFVISAGT 186

Query: 190 VDFI-NLYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCT 249
            D I N+Y     +    S Y+       L++++ V ++ L EAGAR+I   G+ P+GC 
Sbjct: 187 NDMIFNVYDHVLGSLISVSDYQDS-----LLTKVEVFVQRLYEAGARRITIAGLPPIGCL 246

Query: 250 PRVLSLWRDSPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGM 307
           P  ++L   +   I+    C +  N+    YN  ++K I  L++++  +++++ D Y  +
Sbjct: 247 PVQVTLTSINTPRIFHHRICTEHQNDDSRVYNQKLQKLIFGLSQRFRGSKVLYLDIYSPL 301

[Close]

BLAST of Tan0016025 vs. NCBI nr
Match: XP_023533895.1 (GDSL esterase/lipase At1g71250-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 412.5 bits (1059), Expect = 3.2e-111
Identity = 221/305 (72.46%), Postives = 245/305 (80.33%), Query Frame = 0


Query: 1   MEFRIAAIFKPFFFTVHRIFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVD 60
           ME R A +FK  FFT + + LI LL   GL   LAA         +++TAM+LLGDSSVD
Sbjct: 21  MELRSAVLFKSLFFTAY-VTLIGLL---GLS--LAA-------QGSSITAMYLLGDSSVD 80

Query: 61  CGLNTLFYPLLHRNFSLLPCDSNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAILNGLSF 120
           CGLN LFYPLLHRNFS++PCDS+ T LLPHLLAEK+GVP SQP Y QNGSIEAILNGLSF
Sbjct: 81  CGLNALFYPLLHRNFSIIPCDSDGTNLLPHLLAEKMGVPHSQPWYNQNGSIEAILNGLSF 140

Query: 121 GSPQATIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFIN 180
           GSPQATI+S  QS+QSLNQQLRQVLDT+QLLRL+LGLDTARHFIQSS+FY SFGEVDFI 
Sbjct: 141 GSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFI- 200

Query: 181 LYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTPRVLSL 240
              L+SS    N K     F +LL SQM VAIRNL EAGA+KI+CMGILPLGCTPRVLS 
Sbjct: 201 ---LDSSRGKNNLK-----FPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQ 260

Query: 241 WRDSPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGN 300
           WRDSP A+YD+ GCVKEMNELV  YN+LMEKQIV LNEQ GD RM+FCDAYKGMMEIIGN
Sbjct: 261 WRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGN 303

Query: 301 PRHYG 306
           PR YG
Sbjct: 321 PRRYG 303

[Close]

BLAST of Tan0016025 vs. NCBI nr
Match: XP_023533897.1 (GDSL esterase/lipase At1g71250-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 412.5 bits (1059), Expect = 3.2e-111
Identity = 221/305 (72.46%), Postives = 245/305 (80.33%), Query Frame = 0


Query: 1   MEFRIAAIFKPFFFTVHRIFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVD 60
           ME R A +FK  FFT + + LI LL   GL   LAA         +++TAM+LLGDSSVD
Sbjct: 1   MELRSAVLFKSLFFTAY-VTLIGLL---GLS--LAA-------QGSSITAMYLLGDSSVD 60

Query: 61  CGLNTLFYPLLHRNFSLLPCDSNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAILNGLSF 120
           CGLN LFYPLLHRNFS++PCDS+ T LLPHLLAEK+GVP SQP Y QNGSIEAILNGLSF
Sbjct: 61  CGLNALFYPLLHRNFSIIPCDSDGTNLLPHLLAEKMGVPHSQPWYNQNGSIEAILNGLSF 120

Query: 121 GSPQATIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFIN 180
           GSPQATI+S  QS+QSLNQQLRQVLDT+QLLRL+LGLDTARHFIQSS+FY SFGEVDFI 
Sbjct: 121 GSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFI- 180

Query: 181 LYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTPRVLSL 240
              L+SS    N K     F +LL SQM VAIRNL EAGA+KI+CMGILPLGCTPRVLS 
Sbjct: 181 ---LDSSRGKNNLK-----FPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQ 240

Query: 241 WRDSPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGN 300
           WRDSP A+YD+ GCVKEMNELV  YN+LMEKQIV LNEQ GD RM+FCDAYKGMMEIIGN
Sbjct: 241 WRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGN 283

Query: 301 PRHYG 306
           PR YG
Sbjct: 301 PRRYG 283

[Close]

BLAST of Tan0016025 vs. NCBI nr
Match: XP_023533896.1 (GDSL esterase/lipase At1g71250-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 412.5 bits (1059), Expect = 3.2e-111
Identity = 221/305 (72.46%), Postives = 245/305 (80.33%), Query Frame = 0


Query: 1   MEFRIAAIFKPFFFTVHRIFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVD 60
           ME R A +FK  FFT + + LI LL   GL   LAA         +++TAM+LLGDSSVD
Sbjct: 7   MELRSAVLFKSLFFTAY-VTLIGLL---GLS--LAA-------QGSSITAMYLLGDSSVD 66

Query: 61  CGLNTLFYPLLHRNFSLLPCDSNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAILNGLSF 120
           CGLN LFYPLLHRNFS++PCDS+ T LLPHLLAEK+GVP SQP Y QNGSIEAILNGLSF
Sbjct: 67  CGLNALFYPLLHRNFSIIPCDSDGTNLLPHLLAEKMGVPHSQPWYNQNGSIEAILNGLSF 126

Query: 121 GSPQATIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFIN 180
           GSPQATI+S  QS+QSLNQQLRQVLDT+QLLRL+LGLDTARHFIQSS+FY SFGEVDFI 
Sbjct: 127 GSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFI- 186

Query: 181 LYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTPRVLSL 240
              L+SS    N K     F +LL SQM VAIRNL EAGA+KI+CMGILPLGCTPRVLS 
Sbjct: 187 ---LDSSRGKNNLK-----FPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQ 246

Query: 241 WRDSPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGN 300
           WRDSP A+YD+ GCVKEMNELV  YN+LMEKQIV LNEQ GD RM+FCDAYKGMMEIIGN
Sbjct: 247 WRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGN 289

Query: 301 PRHYG 306
           PR YG
Sbjct: 307 PRRYG 289

[Close]

BLAST of Tan0016025 vs. NCBI nr
Match: XP_022958402.1 (GDSL esterase/lipase At1g71250-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 411.0 bits (1055), Expect = 9.3e-111
Identity = 220/302 (72.85%), Postives = 245/302 (81.13%), Query Frame = 0


Query: 4   RIAAIFKPFFFTVHRIFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVDCGL 63
           R AA+FK  FFT + + LI LL   GL   LAA         +++TAM+LLGDSSVDCGL
Sbjct: 25  RSAALFKSLFFTAY-VTLIGLL---GLS--LAA-------QGSSITAMYLLGDSSVDCGL 84

Query: 64  NTLFYPLLHRNFSLLPCDSNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAILNGLSFGSP 123
           N LFYPLLHRNFS++PCDS+ TTLLPHLLAE++GVP SQP Y QNGSIEAILNGLSFGSP
Sbjct: 85  NALFYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSP 144

Query: 124 QATIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFINLYL 183
           QATI+S  QS+QSLNQQLRQVLDT+QLLRL+LGLDTARHFIQSS+FY SFGEVDFI    
Sbjct: 145 QATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFI---- 204

Query: 184 LNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTPRVLSLWRD 243
           L+SS    N K     F +LL SQM VAIRNL EAGA+KI+CMGILPLGCTPRVLS WRD
Sbjct: 205 LDSSRGKNNLK-----FPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRD 264

Query: 244 SPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGNPRH 303
           SP A+YD+ GCVKEMNELV  YN+LMEKQIV LNEQ GD RM+FCDAYKGMMEIIGNPR 
Sbjct: 265 SPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRR 304

Query: 304 YG 306
           YG
Sbjct: 325 YG 304

[Close]

BLAST of Tan0016025 vs. NCBI nr
Match: KAG7035797.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 410.6 bits (1054), Expect = 1.2e-110
Identity = 220/302 (72.85%), Postives = 245/302 (81.13%), Query Frame = 0


Query: 4   RIAAIFKPFFFTVHRIFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVDCGL 63
           R AA+FK  FFT + + LI LL   GL   LAA         +++TAM+LLGDSSVDCGL
Sbjct: 5   RRAALFKSLFFTAY-VTLIGLL---GLS--LAA-------QGSSITAMYLLGDSSVDCGL 64

Query: 64  NTLFYPLLHRNFSLLPCDSNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAILNGLSFGSP 123
           N LFYPLLHRNFS++PCDS+ TTLLPHLLAE++GVP SQP Y QNGSIEAILNGLSFGSP
Sbjct: 65  NALFYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSP 124

Query: 124 QATIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFINLYL 183
           QATI+S  QS+QSLNQQLRQVLDT+QLLRL+LGLDTARHFIQSS+FY SFGEVDFI    
Sbjct: 125 QATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFI---- 184

Query: 184 LNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTPRVLSLWRD 243
           L+SS    N K     F +LL SQM VAIRNL EAGA+KI+CMGILPLGCTPRVLS WRD
Sbjct: 185 LDSSRGKNNLK-----FPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRD 244

Query: 244 SPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGNPRH 303
           SP A+YD+ GCVKEMNELV  YN+LMEKQIV LNEQ GD RM+FCDAYKGMMEIIGNPR 
Sbjct: 245 SPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRR 284

Query: 304 YG 306
           YG
Sbjct: 305 YG 284

[Close]

BLAST of Tan0016025 vs. ExPASy TrEMBL
Match: A0A6J1H1Q6 (GDSL esterase/lipase At1g71250-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459637 PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 4.5e-111
Identity = 220/302 (72.85%), Postives = 245/302 (81.13%), Query Frame = 0


Query: 4   RIAAIFKPFFFTVHRIFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVDCGL 63
           R AA+FK  FFT + + LI LL   GL   LAA         +++TAM+LLGDSSVDCGL
Sbjct: 25  RSAALFKSLFFTAY-VTLIGLL---GLS--LAA-------QGSSITAMYLLGDSSVDCGL 84

Query: 64  NTLFYPLLHRNFSLLPCDSNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAILNGLSFGSP 123
           N LFYPLLHRNFS++PCDS+ TTLLPHLLAE++GVP SQP Y QNGSIEAILNGLSFGSP
Sbjct: 85  NALFYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSP 144

Query: 124 QATIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFINLYL 183
           QATI+S  QS+QSLNQQLRQVLDT+QLLRL+LGLDTARHFIQSS+FY SFGEVDFI    
Sbjct: 145 QATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFI---- 204

Query: 184 LNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTPRVLSLWRD 243
           L+SS    N K     F +LL SQM VAIRNL EAGA+KI+CMGILPLGCTPRVLS WRD
Sbjct: 205 LDSSRGKNNLK-----FPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRD 264

Query: 244 SPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGNPRH 303
           SP A+YD+ GCVKEMNELV  YN+LMEKQIV LNEQ GD RM+FCDAYKGMMEIIGNPR 
Sbjct: 265 SPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRR 304

Query: 304 YG 306
           YG
Sbjct: 325 YG 304

[Close]

BLAST of Tan0016025 vs. ExPASy TrEMBL
Match: A0A6J1K3Z0 (GDSL esterase/lipase At1g71250-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490967 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 1.0e-110
Identity = 221/305 (72.46%), Postives = 244/305 (80.00%), Query Frame = 0


Query: 1   MEFRIAAIFKPFFFTVHRIFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVD 60
           ME R AA+FK  FFT + + LI LL   GL   LAA         +++TAM+LLGDSSVD
Sbjct: 21  MELRRAALFKSLFFTAY-VTLIGLL---GLS--LAA-------RGSSITAMYLLGDSSVD 80

Query: 61  CGLNTLFYPLLHRNFSLLPCDSNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAILNGLSF 120
           CGLN LFYPLLHRNFS+ PCDS+ TTLLPHLLAEK+GVP SQP Y QNGSIEAILNGLSF
Sbjct: 81  CGLNALFYPLLHRNFSITPCDSDGTTLLPHLLAEKMGVPHSQPWYNQNGSIEAILNGLSF 140

Query: 121 GSPQATIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFIN 180
           GSPQATI+S  QS+QSLNQQLRQVLDT+QLLRL+LGLDTARHFIQSS+FY SFGEVDFI 
Sbjct: 141 GSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFI- 200

Query: 181 LYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTPRVLSL 240
              L+SS    N K     F +LL SQM VAIRNL EAGA+KI+CMGILPLGCTPRVLS 
Sbjct: 201 ---LDSSRGKNNVK-----FAQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQ 260

Query: 241 WRDSPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGN 300
           WRDSP A+YD+ GCVKEMNELV  YN+LMEKQIV LN Q GD RM+FCDAYKGMMEII N
Sbjct: 261 WRDSPAAVYDEQGCVKEMNELVVNYNELMEKQIVMLNAQLGDARMVFCDAYKGMMEIISN 303

Query: 301 PRHYG 306
           PR YG
Sbjct: 321 PRRYG 303

[Close]

BLAST of Tan0016025 vs. ExPASy TrEMBL
Match: A0A0A0L1P2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G269190 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 1.0e-110
Identity = 209/274 (76.28%), Postives = 232/274 (84.67%), Query Frame = 0


Query: 33  ILAAAAAANERSNNNVTAMFLLGDSSVDCGLNTLFYPLLHRNFSLLPCDSNQT-TLLPHL 92
           +L    AA E   +NV AMFLLGDSSVDCG NTLFYPLLHRNFSLLPCD+N T +LLP L
Sbjct: 17  LLIDTTAAQE---SNVNAMFLLGDSSVDCGFNTLFYPLLHRNFSLLPCDANATSSLLPFL 76

Query: 93  LAEKIGVPQSQPLYIQNGSIEAILNGLSFGSPQATIMSNAQSYQSLNQQLRQVLDTIQLL 152
           LA+KIG+P +QP Y QN S+EAILNGL+FGSPQATIMSN QSYQSLNQQLRQVLD IQLL
Sbjct: 77  LADKIGLPHAQPFYNQNESLEAILNGLNFGSPQATIMSNEQSYQSLNQQLRQVLDAIQLL 136

Query: 153 RLRLGLDTARHFIQSSIFYLSFGEVDFINLYLLNSSTTSKNSKYEGEEFTRLLVSQMLVA 212
           RL+LG   ARHFIQSS+FYLSFGEVDFINLYLL SS      KY GEEF RLLVSQM++A
Sbjct: 137 RLQLGQHAARHFIQSSLFYLSFGEVDFINLYLLKSS----ERKYGGEEFARLLVSQMVIA 196

Query: 213 IRNLQEAGARKIICMGILPLGCTPRVLSLWRDSPTAIYDKNGCVKEMNELVAKYNDLMEK 272
           IRNLQEAGARKI+CMGILPLGC+PRVLS WRDSP    DK GCVKEMNELV KYN++ME+
Sbjct: 197 IRNLQEAGARKIVCMGILPLGCSPRVLSEWRDSPADTLDKKGCVKEMNELVGKYNEVMEE 256

Query: 273 QIVKLNEQYGDTRMIFCDAYKGMMEIIGNPRHYG 306
           ++VKLN ++GDT+MIFCD YKGMMEIIGNP  YG
Sbjct: 257 EMVKLNAEFGDTQMIFCDVYKGMMEIIGNPTRYG 283

[Close]

BLAST of Tan0016025 vs. ExPASy TrEMBL
Match: A0A6J1H303 (GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459637 PE=3 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 1.4e-109
Identity = 203/260 (78.08%), Postives = 225/260 (86.54%), Query Frame = 0


Query: 46  NNVTAMFLLGDSSVDCGLNTLFYPLLHRNFSLLPCDSNQTTLLPHLLAEKIGVPQSQPLY 105
           +++TAM+LLGDSSVDCGLN LFYPLLHRNFS++PCDS+ TTLLPHLLAE++GVP SQP Y
Sbjct: 5   SSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWY 64

Query: 106 IQNGSIEAILNGLSFGSPQATIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQ 165
            QNGSIEAILNGLSFGSPQATI+S  QS+QSLNQQLRQVLDT+QLLRL+LGLDTARHFIQ
Sbjct: 65  NQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQ 124

Query: 166 SSIFYLSFGEVDFINLYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIIC 225
           SS+FY SFGEVDFI    L+SS    N K     F +LL SQM VAIRNL EAGA+KI+C
Sbjct: 125 SSLFYFSFGEVDFI----LDSSRGKNNLK-----FPQLLASQMSVAIRNLHEAGAKKIVC 184

Query: 226 MGILPLGCTPRVLSLWRDSPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRM 285
           MGILPLGCTPRVLS WRDSP A+YD+ GCVKEMNELV  YN+LMEKQIV LNEQ GD RM
Sbjct: 185 MGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARM 244

Query: 286 IFCDAYKGMMEIIGNPRHYG 306
           +FCDAYKGMMEIIGNPR YG
Sbjct: 245 VFCDAYKGMMEIIGNPRRYG 255

[Close]

BLAST of Tan0016025 vs. ExPASy TrEMBL
Match: A0A5A7TDD0 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00690 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.5e-109
Identity = 202/261 (77.39%), Postives = 226/261 (86.59%), Query Frame = 0


Query: 46  NNVTAMFLLGDSSVDCGLNTLFYPLLHRNFSLLPCDSNQT-TLLPHLLAEKIGVPQSQPL 105
           +NVTAMFLLGDSSVDCG NTLFYP LHRNFSLLPCD+N T +LLP LLA+KIG+P +QP 
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNATSSLLPFLLADKIGLPHAQPW 80

Query: 106 YIQNGSIEAILNGLSFGSPQATIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFI 165
           Y QNGS+EAILNGL+FGSPQATIMSN QSYQSLNQQLRQVLD IQLLRL+LG   ARHFI
Sbjct: 81  YNQNGSLEAILNGLNFGSPQATIMSNGQSYQSLNQQLRQVLDAIQLLRLQLGQHAARHFI 140

Query: 166 QSSIFYLSFGEVDFINLYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKII 225
           QSS+FYLSFGEVDFINLYLL SS      KY GEEF RLLVSQM++AIRNLQEAGARKI+
Sbjct: 141 QSSLFYLSFGEVDFINLYLLKSSA----RKYGGEEFARLLVSQMVIAIRNLQEAGARKIV 200

Query: 226 CMGILPLGCTPRVLSLWRDSPTAIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTR 285
           CMGILPLGC+PRVLS WRDSP   +DK GCVKEMNEL+ KYN++M +++VKLN ++ DT+
Sbjct: 201 CMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDTQ 260

Query: 286 MIFCDAYKGMMEIIGNPRHYG 306
           MIFCD YKGM EIIGNP  YG
Sbjct: 261 MIFCDVYKGMTEIIGNPTRYG 277

[Close]

BLAST of Tan0016025 vs. TAIR 10
Match: AT4G10950.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.5e-26
Identity = 77/270 (28.52%), Postives = 133/270 (49.26%), Query Frame = 0


Query: 48  VTAMFLLGDSSVDCGLNTLFYPLLHRNFSLLPCDSNQTTLLP-------HLLAEKIGVPQ 107
           V A+F+ GDSSVD G N     L   +   LP   +  T  P        +  + +G+P 
Sbjct: 69  VPALFVFGDSSVDSGTNNFLGTLARAD--RLPYGRDFDTHQPTGRFCNGRIPVDYLGLPF 128

Query: 108 SQPLYIQNGSIEAILNGLSFGSPQATIMSNAQS----YQSLNQQLRQVLDTIQLLRLRLG 167
                 Q G++E +  G+++ S  A I+ ++ S      S   Q+ Q +DT Q + L +G
Sbjct: 129 VPSYLGQTGTVEDMFQGVNYASAGAGIILSSGSELGQRVSFAMQVEQFVDTFQQMILSIG 188

Query: 168 LDTARHFIQSSIFYLSFGEVDFINLYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQ 227
              +   + +S+FY+S G  D+I+ Y+ N S     + Y    F + L S M   ++ L 
Sbjct: 189 EKASERLVSNSVFYISIGVNDYIHFYIRNISNV--QNLYTPWNFNQFLASNMRQELKTLY 248

Query: 228 EAGARKIICMGILPLGCTPRVLSLWRDSPTAIYDKNG-CVKEMNELVAKYNDLMEKQIVK 287
               R+++ MG+ P+GC P  +  +R        +NG C +E+N ++ + N +M   + K
Sbjct: 249 NVKVRRMVVMGLPPIGCAPYYMWKYR-------SQNGECAEEVNSMIMESNFVMRYTVDK 308

Query: 288 LNEQYGDTRMIFCDAYKGMMEIIGNPRHYG 306
           LN +     +I+CD ++  M+I+ N +HYG
Sbjct: 309 LNRELPGASIIYCDVFQSAMDILRNHQHYG 327

[Close]

BLAST of Tan0016025 vs. TAIR 10
Match: AT5G37690.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 4.6e-23
Identity = 76/299 (25.42%), Postives = 144/299 (48.16%), Query Frame = 0


Query: 24  LLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVDCGLNT-LFYPLLHRNFSLLPCD- 83
           ++LR+ L  +++  A A   S +++   ++ GDS  + G N  L Y L   +F     D 
Sbjct: 2   MILRLALAIVISTYATAQPASTSSLVT-YIFGDSLTEVGNNNFLQYSLARADFPYYGVDF 61

Query: 84  ---------SNQTTLLPHLLAEKIGVPQSQPLYIQNGSIEAILNGLSFGSPQATIMSNAQ 143
                    +N  T +  +++ K+G+    P    + + +A L+G+++ S  A I++   
Sbjct: 62  SGGKATGRFTNGRT-IGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETG 121

Query: 144 SY----QSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFINLYLLNSST 203
            Y     + N Q+     T +++R ++G   A   +  +++++  G  D++N +L     
Sbjct: 122 IYFIQRLTFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFL--QPF 181

Query: 204 TSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTP--RVLSLWRDSPT 263
            +   +Y  +EF  LL S +   +  + + GARK+I  G+ PLGC P  RV S  R    
Sbjct: 182 MADGQQYTHDEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTR---- 241

Query: 264 AIYDKNGCVKEMNELVAKYNDLMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGNPRHYG 306
                  C+  +NE V ++N   +K ++ LN++    +  F D Y  ++++I NP HYG
Sbjct: 242 ------MCLNRVNEWVLEFNSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYG 286

[Close]

BLAST of Tan0016025 vs. TAIR 10
Match: AT5G45950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 91.7 bits (226), Expect = 1.2e-18
Identity = 78/280 (27.86%), Postives = 132/280 (47.14%), Query Frame = 0


Query: 44  SNNNVTAMFLLGDSSVDCGLNTLFYPLLHRNFSLLPCDSN-----------QTTLLPHLL 103
           + +NVT++ + GDSSVD G N      +  NF   P   N              L P  +
Sbjct: 34  AKHNVTSILVFGDSSVDPGNNNFIKTEMKGNFP--PYGENFINHKPTGRLCDGLLAPDYI 93

Query: 104 AEKIGVPQSQPLYIQNGSIEA-ILNGLSF---GSPQATIMSNAQSYQSLNQQLRQVLD-T 163
           AE +G P   P ++     +A +  G SF   GS    + +N  +  S   Q    L   
Sbjct: 94  AEAMGYPPI-PAFLDPSLTQADLTRGASFASAGSGYDDLTANISNVWSFTTQANYFLHYK 153

Query: 164 IQLLRLRLGLDTARHFIQSSIFYLSFGEVDFINLYLLNSSTTSKNSKYEGEEFTRLLVSQ 223
           I L +L   L++A+  I ++IF +S G  DF+  YL++    ++  ++  E++   L  +
Sbjct: 154 IHLTKLVGPLESAK-MINNAIFLMSMGSNDFLQNYLVD---FTRQKQFTVEQYIEFLSHR 213

Query: 224 MLVAIRNLQEAGARKIICMGILPLGCTPRVLSLWRDSPTAIYDKNGCVKEMNELVAKYND 283
           ML   + L   GA++++ +G+ P+GC P +  L R   T       CV ++N++   +N 
Sbjct: 214 MLYDAKMLHRLGAKRLVVVGVPPMGCMPLIKYL-RGQKT-------CVDQLNQIAFSFNA 273

Query: 284 LMEKQIVKLNEQYGDTRMIFCDAYKGMMEIIGNPRHYGML 308
            + K +  L  + G  + I+ DAY  + E I NPR +G +
Sbjct: 274 KIIKNLELLQSKIG-LKTIYVDAYSTIQEAIKNPRKFGFV 297

[Close]

BLAST of Tan0016025 vs. TAIR 10
Match: AT5G15720.1 (GDSL-motif lipase 7 )

HSP 1 Score: 90.5 bits (223), Expect = 2.6e-18
Identity = 76/274 (27.74%), Postives = 128/274 (46.72%), Query Frame = 0


Query: 50  AMFLLGDSSVDCGLNTLFYPLLHRNFSLLPCD--------SNQTTLLPHLLAEKIGVPQS 109
           A F+ GDS VD G N     L   N+     D         N  T++ +  A  +G+P  
Sbjct: 30  AFFVFGDSLVDSGNNNYIPTLARANYFPYGIDFGFPTGRFCNGRTVVDY-GATYLGLPLV 89

Query: 110 QPLYIQNGSI-EAILNGLSFGSPQATIMSNAQSY----QSLNQQLRQVLDTIQLLRLRLG 169
            P Y+   SI +  L G+++ S  A I+     +     + N Q+ Q   TI+L   R  
Sbjct: 90  PP-YLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFF 149

Query: 170 LDTA--RHFIQSSIFYLSFGEVDFINLYLLNSSTTSKNSKYEGEEFTRLLVSQMLVAIRN 229
            + A  R ++  SI  ++ G  D+IN YL+     S +  Y GE++  LL+  +   I  
Sbjct: 150 QNPADLRKYLAKSIIGINIGSNDYINNYLM-PERYSTSQTYSGEDYADLLIKTLSAQISR 209

Query: 230 LQEAGARKIICMGILPLGCTPRVLSLWRDSPTAIYDKNGCVKEMNELVAKYNDLMEKQIV 289
           L   GARK++  G  PLGC P  LS+   + T     +GCV ++N +V+ +N  ++    
Sbjct: 210 LYNLGARKMVLAGSGPLGCIPSQLSMVTGNNT-----SGCVTKINNMVSMFNSRLKDLAN 269

Query: 290 KLNEQYGDTRMIFCDAYKGMMEIIGNPRHYGMLL 309
            LN     +  ++ + +    +++ NP  YG+++
Sbjct: 270 TLNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVV 295

[Close]

BLAST of Tan0016025 vs. TAIR 10
Match: AT5G45960.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 1.3e-17
Identity = 72/302 (23.84%), Postives = 140/302 (46.36%), Query Frame = 0


Query: 19  IFLIALLLRMGLDNILAAAAAANERSNNNVTAMFLLGDSSVDCGLNTLFYPLLHRNFSLL 78
           +FL    +           A +  +  ++V+A+ + GDS+VD G N     +   NF   
Sbjct: 16  LFLFLFFISFSSSTSKLEPAKSEPKRKHSVSAILVFGDSTVDPGNNNYIDTVFKCNFPPY 75

Query: 79  PCDSNQTT---------LLPHLLAEKIGVPQSQPLYIQ-NGSIEAILNGLSF---GSPQA 138
             D    T         L+   +A  IGV ++ P Y+  N  I  +++G+SF   GS   
Sbjct: 76  GLDFRNKTPTGRFCNGRLVTDFIASYIGVKENVPPYLDPNLGINELISGVSFASAGSGYD 135

Query: 139 TIMSNAQSYQSLNQQLRQVLDTIQLLRLRLGLDTARHFIQSSIFYLSFGEVDFINLYLLN 198
            +     +   +  QL    +  + L  ++G       I+ ++F +S G  DF+  Y   
Sbjct: 136 PLTPTITNVIDIPTQLEYFREYKRKLEGKMGKQEMEKHIEEAMFCVSAGTNDFVINYF-- 195

Query: 199 SSTTSKNSKYEGEEFTRLLVSQMLVAIRNLQEAGARKIICMGILPLGCTPRVLSLWRDSP 258
            +   +   +  E + + ++S +   I+ L + GARKI   G+ P+GC P V++L+  S 
Sbjct: 196 -TIPIRRKTFTIEAYQQFVISNLKQFIQGLWKEGARKITVAGLPPIGCLPIVITLF--SG 255

Query: 259 TAIYDKNGCVKEMNELVAKYNDLMEKQ--IVKLNEQYGDTRMIFCDAYKGMMEIIGNPRH 306
            A+ ++  C+   + +   YN L++KQ  ++++   +  +++ + D Y  + E+I +PR 
Sbjct: 256 EALTNRR-CIDRFSTVATNYNFLLQKQLALMQVGLAHLGSKIFYLDVYNPVYEVIRDPRK 311

The following BLAST results are available for this feature:

BLAST of Tan0016025 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHQ1	6.4e-22	25.42	GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2...	[more]
Q9FJ41	1.6e-17	27.86	GDSL esterase/lipase At5g45950 OS=Arabidopsis thaliana OX=3702 GN=At5g45950 PE=2...	[more]
Q8LFJ9	3.6e-17	27.74	GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1	[more]
Q9FJ40	1.8e-16	23.84	GDSL esterase/lipase At5g45960 OS=Arabidopsis thaliana OX=3702 GN=At5g45960 PE=2...	[more]
Q9SIZ6	4.0e-16	25.32	GDSL esterase/lipase At2g40250 OS=Arabidopsis thaliana OX=3702 GN=At2g40250 PE=2...	[more]

BLAST of Tan0016025 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023533895.1	3.2e-111	72.46	GDSL esterase/lipase At1g71250-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023533897.1	3.2e-111	72.46	GDSL esterase/lipase At1g71250-like isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_023533896.1	3.2e-111	72.46	GDSL esterase/lipase At1g71250-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022958402.1	9.3e-111	72.85	GDSL esterase/lipase At1g71250-like isoform X1 [Cucurbita moschata]	[more]
KAG7035797.1	1.2e-110	72.85	GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Tan0016025 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H1Q6	4.5e-111	72.85	GDSL esterase/lipase At1g71250-like isoform X1 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1K3Z0	1.0e-110	72.46	GDSL esterase/lipase At1g71250-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A0A0L1P2	1.0e-110	76.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G269190 PE=3 SV=1	[more]
A0A6J1H303	1.4e-109	78.08	GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A5A7TDD0	2.5e-109	77.39	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366...	[more]

BLAST of Tan0016025 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10950.1	1.5e-26	28.52	SGNH hydrolase-type esterase superfamily protein	[more]
AT5G37690.1	4.6e-23	25.42	SGNH hydrolase-type esterase superfamily protein	[more]
AT5G45950.1	1.2e-18	27.86	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G15720.1	2.6e-18	27.74	GDSL-motif lipase 7	[more]
AT5G45960.1	1.3e-17	23.84	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 134..154
None	No IPR available	PANTHER	PTHR45648	GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)	coord: 34..305
None	No IPR available	PANTHER	PTHR45648:SF7	OS12G0126100 PROTEIN	coord: 34..305
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 37..308 e-value: 1.8E-34 score: 121.6
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 53..292 e-value: 5.4E-6 score: 27.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0016025.1	Tan0016025.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds