Tan0015985 (gene) Snake gourd v1

Overview
NameTan0015985
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptioncalmodulin-like
LocationLG02: 85620465 .. 85621086 (+)
RNA-Seq ExpressionTan0015985
SyntenyTan0015985
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTACATTGATCACCAAGCAAAAGAAAATAAACACAAACGTTTGCAAAACACTAAAATCAGAAAATGTTTCCTGCATCAAGCAAGCTACATTTCTCCAGGGGGGAGATCAAGGAAATCTTCAGAGAGCACGATATCGATGGAGATGGTCGATTGAGCATCAGTGAGCTCGTGAAGGCCTTCGGGTTCTTCGGCAGTATCCTTCCTTTCTACAAAGCTCACTATGGTTTGGCTTTTGCCGATACTGATTGTGATGGCTACATCGATGAGGATGAGCTCGACAAGCTCGTCGACTATGCCGAGAAGTTTCAGCACAAGAGGCACTGCGGCTAATAATGTAACATCCTCAGTTTGCTAATAATGTTCCGTTTGAATAAAAAGAAAAAGAGGGTTGGCTAGCTAGCTAGTCCTGCTTTGGTTTGGCTATTAAATGGGAAGTGCTTCCATTGATCGAATTATATATTGTTTGTGTTTAAAAAAAAATATGGTCTCGTTTGATAACATTTCCATTTTTTGTTTCCTGTTTTCCAATTTTTAAGAATAAGAAACAATAATATGTTTGATAACTATTTCTGTTTCTTGGTTCTTAAAAACAAGAAACAAAAACAGAAGTTTATTTGATAA

mRNA sequence

CTTACATTGATCACCAAGCAAAAGAAAATAAACACAAACGTTTGCAAAACACTAAAATCAGAAAATGTTTCCTGCATCAAGCAAGCTACATTTCTCCAGGGGGGAGATCAAGGAAATCTTCAGAGAGCACGATATCGATGGAGATGGTCGATTGAGCATCAGTGAGCTCGTGAAGGCCTTCGGGTTCTTCGGCAGTATCCTTCCTTTCTACAAAGCTCACTATGGTTTGGCTTTTGCCGATACTGATTGTGATGGCTACATCGATGAGGATGAGCTCGACAAGCTCGTCGACTATGCCGAGAAGTTTCAGCACAAGAGGCACTGCGGCTAATAATGTAACATCCTCAGTTTGCTAATAATGTTCCGTTTGAATAAAAAGAAAAAGAGGGTTGGCTAGCTAGCTAGTCCTGCTTTGGTTTGGCTATTAAATGGGAAGTGCTTCCATTGATCGAATTATATATTGTTTGTGTTTAAAAAAAAATATGGTCTCGTTTGATAACATTTCCATTTTTTGTTTCCTGTTTTCCAATTTTTAAGAATAAGAAACAATAATATGTTTGATAACTATTTCTGTTTCTTGGTTCTTAAAAACAAGAAACAAAAACAGAAGTTTATTTGATAA

Coding sequence (CDS)

ATGTTTCCTGCATCAAGCAAGCTACATTTCTCCAGGGGGGAGATCAAGGAAATCTTCAGAGAGCACGATATCGATGGAGATGGTCGATTGAGCATCAGTGAGCTCGTGAAGGCCTTCGGGTTCTTCGGCAGTATCCTTCCTTTCTACAAAGCTCACTATGGTTTGGCTTTTGCCGATACTGATTGTGATGGCTACATCGATGAGGATGAGCTCGACAAGCTCGTCGACTATGCCGAGAAGTTTCAGCACAAGAGGCACTGCGGCTAA

Protein sequence

MFPASSKLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDGYIDEDELDKLVDYAEKFQHKRHCG
Homology
BLAST of Tan0015985 vs. ExPASy Swiss-Prot
Match: Q9C9U8 (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 2.2e-04
Identity = 21/57 (36.84%), Postives = 36/57 (63.16%), Query Frame = 0

Query: 14 EIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDGYIDEDE 71
          E+K++F + D +GDG++S+SEL   F   G+     + +  L   D DCDG+I+++E
Sbjct: 20 ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEE 76

BLAST of Tan0015985 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 2.9e-04
Identity = 28/69 (40.58%), Postives = 39/69 (56.52%), Query Frame = 0

Query: 14 EIKEIFREHDIDGDGRLSISEL---VKAFGFFGSILPFYKAHYGLAFADTDCDGYIDEDE 73
          E + +F   D DGDG++S +EL   VKA    G  +P  +    +A ADTD DG +DE+E
Sbjct: 6  EFRRVFGSFDQDGDGKISATELRLCVKA--SLGEDMPDEEVQALMALADTDGDGLLDEEE 65

Query: 74 LDKLVDYAE 80
            +LV   E
Sbjct: 66 FVRLVTEME 72

BLAST of Tan0015985 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 8.4e-04
Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 0

Query: 14 EIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDGYIDEDELDK 73
          E+K++F + D +GDG++S+ EL   F   G+     + +  L   DTD DGYI+ DE   
Sbjct: 23 ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 74 L 75
          L
Sbjct: 83 L 83

BLAST of Tan0015985 vs. NCBI nr
Match: KAA0060892.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 148.3 bits (373), Expect = 3.1e-32
Identity = 70/87 (80.46%), Postives = 78/87 (89.66%), Query Frame = 0

Query: 1  MFPASSKLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADT 60
          M  A+SKL  SR +IKEIFREHDIDGDGRLS+SE+VKAFGFFGSILPFYKAHYG+A AD 
Sbjct: 1  MSRAASKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADE 60

Query: 61 DCDGYIDEDELDKLVDYAEKFQHKRHC 88
          + DGYI+E+ELDKLVDYAEKFQHKR C
Sbjct: 61 NGDGYIEEEELDKLVDYAEKFQHKRKC 87

BLAST of Tan0015985 vs. NCBI nr
Match: KGN62516.1 (hypothetical protein Csa_022521 [Cucumis sativus])

HSP 1 Score: 138.7 bits (348), Expect = 2.5e-29
Identity = 64/87 (73.56%), Postives = 77/87 (88.51%), Query Frame = 0

Query: 1  MFPASSKLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADT 60
          M  A+++L  SR +IKEIFREHDIDGDGRLS++E+VKAFG FGSILPFYKAHYG+A AD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DCDGYIDEDELDKLVDYAEKFQHKRHC 88
          + DG+I+E+ELDKLVDYA+KFQHKR C
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKRKC 87

BLAST of Tan0015985 vs. NCBI nr
Match: XP_004152805.1 (probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 137.5 bits (345), Expect = 5.5e-29
Identity = 63/83 (75.90%), Postives = 75/83 (90.36%), Query Frame = 0

Query: 5   SSKLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDG 64
           S++L  SR +IKEIFREHDIDGDGRLS++E+VKAFG FGSILPFYKAHYG+A AD + DG
Sbjct: 86  STRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 145

Query: 65  YIDEDELDKLVDYAEKFQHKRHC 88
           +I+E+ELDKLVDYA+KFQHKR C
Sbjct: 146 FIEEEELDKLVDYAQKFQHKRKC 168

BLAST of Tan0015985 vs. NCBI nr
Match: KGN62525.1 (hypothetical protein Csa_022453 [Cucumis sativus])

HSP 1 Score: 126.3 bits (316), Expect = 1.3e-25
Identity = 58/83 (69.88%), Postives = 68/83 (81.93%), Query Frame = 0

Query: 3  PASSKLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDC 62
          P  SK +FSR E+KEIFREHDIDGDG LSISEL+KA GF G  +PFYKAHYG+A+ D + 
Sbjct: 4  PGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENG 63

Query: 63 DGYIDEDELDKLVDYAEKFQHKR 86
          DG I EDELDKL+DYAE+FQ +R
Sbjct: 64 DGLISEDELDKLIDYAERFQKRR 86

BLAST of Tan0015985 vs. NCBI nr
Match: XP_022144156.1 (probable calcium-binding protein CML15 [Momordica charantia])

HSP 1 Score: 120.6 bits (301), Expect = 7.0e-24
Identity = 56/74 (75.68%), Postives = 63/74 (85.14%), Query Frame = 0

Query: 7   KLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDGYI 66
           K+ FSR +IKEIF EHDIDGDG LS+SEL+KAF F GS++P YKAHYGLAFAD D DG I
Sbjct: 70  KIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDGLI 129

Query: 67  DEDELDKLVDYAEK 81
            E+ELDKLVDYAEK
Sbjct: 130 SEEELDKLVDYAEK 143

BLAST of Tan0015985 vs. ExPASy TrEMBL
Match: A0A5A7UYC0 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00250 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-32
Identity = 70/87 (80.46%), Postives = 78/87 (89.66%), Query Frame = 0

Query: 1  MFPASSKLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADT 60
          M  A+SKL  SR +IKEIFREHDIDGDGRLS+SE+VKAFGFFGSILPFYKAHYG+A AD 
Sbjct: 1  MSRAASKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADE 60

Query: 61 DCDGYIDEDELDKLVDYAEKFQHKRHC 88
          + DGYI+E+ELDKLVDYAEKFQHKR C
Sbjct: 61 NGDGYIEEEELDKLVDYAEKFQHKRKC 87

BLAST of Tan0015985 vs. ExPASy TrEMBL
Match: A0A0A0LRC1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.2e-29
Identity = 64/87 (73.56%), Postives = 77/87 (88.51%), Query Frame = 0

Query: 1  MFPASSKLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADT 60
          M  A+++L  SR +IKEIFREHDIDGDGRLS++E+VKAFG FGSILPFYKAHYG+A AD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DCDGYIDEDELDKLVDYAEKFQHKRHC 88
          + DG+I+E+ELDKLVDYA+KFQHKR C
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKRKC 87

BLAST of Tan0015985 vs. ExPASy TrEMBL
Match: A0A0A0LKX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 6.2e-26
Identity = 58/83 (69.88%), Postives = 68/83 (81.93%), Query Frame = 0

Query: 3  PASSKLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDC 62
          P  SK +FSR E+KEIFREHDIDGDG LSISEL+KA GF G  +PFYKAHYG+A+ D + 
Sbjct: 4  PGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENG 63

Query: 63 DGYIDEDELDKLVDYAEKFQHKR 86
          DG I EDELDKL+DYAE+FQ +R
Sbjct: 64 DGLISEDELDKLIDYAERFQKRR 86

BLAST of Tan0015985 vs. ExPASy TrEMBL
Match: A0A6J1CSH1 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111013915 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.4e-24
Identity = 56/74 (75.68%), Postives = 63/74 (85.14%), Query Frame = 0

Query: 7   KLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDGYI 66
           K+ FSR +IKEIF EHDIDGDG LS+SEL+KAF F GS++P YKAHYGLAFAD D DG I
Sbjct: 70  KIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDGLI 129

Query: 67  DEDELDKLVDYAEK 81
            E+ELDKLVDYAEK
Sbjct: 130 SEEELDKLVDYAEK 143

BLAST of Tan0015985 vs. ExPASy TrEMBL
Match: A0A6J1KUW2 (calmodulin-like OS=Cucurbita maxima OX=3661 GN=LOC111496626 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.9e-23
Identity = 58/82 (70.73%), Postives = 66/82 (80.49%), Query Frame = 0

Query: 4   ASSKLHFSRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCD 63
           AS KL  SR +IKEIF EHDIDGDG LS+SEL KAF FFGSI+P YKAHYGLA+AD D D
Sbjct: 81  ASMKL--SRSQIKEIFMEHDIDGDGYLSVSELTKAFSFFGSIIPLYKAHYGLAYADADGD 140

Query: 64  GYIDEDELDKLVDYAEKFQHKR 86
           G I E+EL+KLVDYAE+   K+
Sbjct: 141 GLISEEELEKLVDYAERTNKKK 160

BLAST of Tan0015985 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 46.2 bits (108), Expect = 1.6e-05
Identity = 21/57 (36.84%), Postives = 36/57 (63.16%), Query Frame = 0

Query: 14 EIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDGYIDEDE 71
          E+K++F + D +GDG++S+SEL   F   G+     + +  L   D DCDG+I+++E
Sbjct: 20 ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEE 76

BLAST of Tan0015985 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 44.3 bits (103), Expect = 6.0e-05
Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 0

Query: 14 EIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDGYIDEDELDK 73
          E+K++F + D +GDG++S+ EL   F   G+     + +  L   DTD DGYI+ DE   
Sbjct: 23 ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 74 L 75
          L
Sbjct: 83 L 83

BLAST of Tan0015985 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 44.3 bits (103), Expect = 6.0e-05
Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 0

Query: 14 EIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDGYIDEDELDK 73
          E+K++F + D +GDG++S+ EL   F   G+     + +  L   DTD DGYI+ DE   
Sbjct: 23 ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 74 L 75
          L
Sbjct: 83 L 83

BLAST of Tan0015985 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 42.4 bits (98), Expect = 2.3e-04
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 0

Query: 11  SRGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDG 65
           S+ E++++F   D DGDG++S  EL   FG  G  +    A   +   DTD DG
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADG 114

BLAST of Tan0015985 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 41.6 bits (96), Expect = 3.9e-04
Identity = 24/73 (32.88%), Postives = 40/73 (54.79%), Query Frame = 0

Query: 12 RGEIKEIFREHDIDGDGRLSISELVKAFGFFGSILPFYKAHYGLAFADTDCDGYIDEDEL 71
          RGE+  +F+  D +GDG+++ +EL   F   G ++P  + +  +A  D + DG +D DE 
Sbjct: 3  RGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF 62

Query: 72 DKLVDYAEKFQHK 85
            L  Y E  + K
Sbjct: 63 GSL--YQEMVEEK 73

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9U82.2e-0436.84Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ... [more]
Q8RYJ92.9e-0440.58Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9LE228.4e-0437.70Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ... [more]
Match NameE-valueIdentityDescription
KAA0060892.13.1e-3280.46putative calcium-binding protein CML10 [Cucumis melo var. makuwa][more]
KGN62516.12.5e-2973.56hypothetical protein Csa_022521 [Cucumis sativus][more]
XP_004152805.15.5e-2975.90probable calcium-binding protein CML10 [Cucumis sativus][more]
KGN62525.11.3e-2569.88hypothetical protein Csa_022453 [Cucumis sativus][more]
XP_022144156.17.0e-2475.68probable calcium-binding protein CML15 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A5A7UYC01.5e-3280.46Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A0A0LRC11.2e-2973.56Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1[more]
A0A0A0LKX16.2e-2669.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359940 PE=4 SV=1[more]
A0A6J1CSH13.4e-2475.68probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1KUW22.9e-2370.73calmodulin-like OS=Cucurbita maxima OX=3661 GN=LOC111496626 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G73630.11.6e-0536.84EF hand calcium-binding protein family [more]
AT1G18210.16.0e-0537.70Calcium-binding EF-hand family protein [more]
AT1G18210.26.0e-0537.70Calcium-binding EF-hand family protein [more]
AT3G50770.12.3e-0437.04calmodulin-like 41 [more]
AT4G12860.13.9e-0432.88EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 14..42
e-value: 0.0028
score: 26.9
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 14..39
e-value: 1.6E-7
score: 30.4
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 58..71
e-value: 0.021
score: 14.6
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 10..45
score: 13.564962
NoneNo IPR availableGENE3D1.10.238.10coord: 10..85
e-value: 4.5E-11
score: 44.2
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 59..71
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 23..35
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 6..74

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0015985.1Tan0015985.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding