Tan0015974 (gene) Snake gourd v1

Overview
NameTan0015974
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionRhomboid domain-containing protein
LocationLG04: 57503246 .. 57507983 (+)
RNA-Seq ExpressionTan0015974
SyntenyTan0015974
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCTTCTTAACTCCAAAAACCACTTAGGTGTTTAATCACCTTCAAAAATCCTCCCTTTCGATCATTAGCTCAAATATACACTCACGTCCAAAATGATGGCTGTTGTAATTCCCACCCTTTGCCACACACGACACCTTAACAACTCGATCAATCCAACCAAACAAATCATTTTTGGATACAATAAGAGACCTACGACTTGCAAATACGATCTTGTTTTAAAGAATTTGCCAATTTTTGAGTGTCGTAAACATTGGCAAGAGATTTCAAATAACGATTCATTTGTTGTTATGGTCTCGGCAAGAACGAAAAAGTCGAGAAGATGGTCGAAGGCGCTATTGCAAAGTGATGAAAGGCATGTGAGATGCAGTGGTTTGGATTTTGAATCACAAGTTTTCAAAAAACTATGCCATGTTTGGGAGTGTAGTACTAATGAGAAACAGTTGAGATCATTGGGTTCTTATTTTGGAAGGCTTCAAGGTGGTGGAAATAAGAAAAAAATGGATTCTTTGAAGAAGATAGAGGTGCTTGATACTGGTCAGTTTAAAGCAAAGAAGGAGCTTGAGTTGCTTGATGCTTATTTTGAAAAAGTTGATAAAGGTAACTCTTTCTCCCTATCATATGATTTTGTATTATGTTTTTCATGACTTAAATCTCCATAAATATTGTTGAATTACTGTCAAGTTCAAAAGTTTAAGTGGATAATTATTCTATGTTTTGTTATTTTATATATTCTGTCATCCGCCCCTTTCATGGATATTAGTTTAATGGTGTAAATCATTTTTGGGCTTTTAAACTTTCATACTTATTTTATTTTGGTCCTTAAACTTTTAGTTATTCTATTTTAATCCATCAACTTTGCATGAAAAGACATTATTTTAGTCTCCTTTTTTTTTAATAATAAGTTACTTTTTAGTCTTTATCATTAATTTTCGTAGTTATTTATAATTTGTTTTGAAGCATACCTAAACCTATATATACATATATATAATTATAAATACTCAATCATGCAGATCTCTCTACTCTCTAGATAAATGAATATAAAAAAACATGCAAATACATTAGGGTTTTGTAAAATTAAAATAATTACAAAAGAAAGTTAACAACTTTGAAATGGTTTTTTTTAATGCAAAGTTAAAGCACCAAAACCCATTTTGAAATTGAAGAACCAAAAATTGAACCAACGAAAAAATTTAGGGCTTGTTTAGTAGGTAATTCAGATTCTGTTTTATGTTTTTAGATTCACTGAATTCAGTGAATACGTGTTTGGCAGACATGTTTTAAAATCTGCTTTGGATTTCATGATCCACATAGTAGAAATTACAAAACAACATTTTTATGTTTTCATTGTATCCAGATTCTGAATTTGAAAATTTAAAATGCAGGATTATTTTAAACAAAGATAAAAATCTTAATAAATATAATATTACATATTTATAAACTTAATTTATTTTTTTAATTAAAATATGTATAATATAATATATTATAAAATTTAAAATATTATATTATAAAATAATAATTATATAAAATTTATATTAAAAAAAACATGTTCATAATTAATTAATAATGTCTTATATTCAACCTAATATTTAGTTTTGCAACTACAACTAATTTTATGATCTATAAATATATTATAGAACAAATTTTAAACTATCGCTTAAAATGAAATCAATTTTCTAAATCACTACTAAAACACATATCCAAAACATGAAATACAACTTTGTTTTCATTGAATCCTTGTTTTCAAATTTCTATTTTTGGATTGCTCACCAAACAATGCTTCTCAAAAGATTGAAATATAAGATTTAAACTTATCTAATTTAATTCATGAAATACTTAGATAGTAACATGAATCAGATAACATCCATCTTGCAAAATGATTTGTAAATCATGATACTTCAAAACATTAAACTTAAGTTGATAGATTTGGATAAATTTAATCACATAGTAAACCCAAACACCCTTTCAATATTTTGCGAGCATTATTTCCGAATTTGATATTTAAAATTCCAATAGATCTAGATACGCACAACCATGCTCGGTCTAATACTTTTGGAGAACAACATGAAGGTAAATCAGTCTTCACATCATTCCCTTTTGAAGATTGCGCTAGAGATGACGAGATACAGATACGACACGAGAAACCGAATGATATGATGCATAGAAGCATTCCAAAAACAACTCAAATGCAAGAAGATGAAGTTTGTGATCTCTACTTGATGTAAAGTTCAAACCTTAACTCATTAGATTTTCAGTTAGTTTTAGATGACTTTTGATTAAAGTGCTGTGGAGTGAAAGTACTTCTAAACTTAGAAGTCACGATTGGTTAAAATCACTTTTGATTATCTTAGCAAGTACTACAAAAATTGTTTACATACATTTTTAATGAACTAAAAGTACTTTTTAAAAAGTTATTCCAAACTCATCTCTATTGATGTTTGATACAAAATGTATGTTCGAATATATTGTCGCTAACGGTACCGTGTTCGATTATGCAGAAGTACTCTGGTTTCTGTAAACATAGCAGTTTTCTTGTTTGAAGTAGCAAGTCCAGTAAAAAACTCAGACCTGCCACTCTTTTCACTCCCTTCTCTATATGGAGCAAAGATAAATGACCTGATTCTAGTAGGAGAATGGTGGAGGCTTGTGACACCTATGTTTCTGGTACAAGATTGATACGACTCGGTTCGTGTTTCTGTTCTAGTGTTGTTTTCGACGTTTATTCATTTGTCTTTCTCAATAAACATCATGAACAAAAGAGTTTTCTCCTACTTTGAACATAGTTCAACTGGTCAAGACATTATAACATTCCTTATAAAGTCGAGTTGAGTTATTTTTAATGGGATTTTTGTATATTTTAAGACACTCTTCTTTTGACATTGAAGATTTGAATGTACTCAAAATGCCTTGCTTATTACTTGCTTAGATCTCATGAAGGTGATCTCTTTATGCAGCATTCAGGAGTTCTTCATGTTGCTCTCAGTTGTTGGACACTTCTTACATTTGGCCGTCAAGTTTGTAGAGACTATGGTCCCTTCACATTTTTCTTGATCTATGTCCTTGGAGGAGTTTCTGGCAACCTCACAAGCTTTCTTCATACTCCCGAGCCAACTGTCGGTGGCACGGTAACCAAAAAATAACATTTCTTCTTTTCGTTTCCAATCACGATTAGTAAATTTATTCTCGTGACTTGATATATCCATATCTCATGTTGTTGAGTCACCAATTATATTAAGAGAGCATGTTTGCAATGACTTTTTAAAAGCTTAAAAATACTTTTTTAAGTACTTAGAAAGTCATTTCAAACGGTTACACTACCTATTTAGAATATTAAATACCCCAAGTATTATATAATACCAAATGTTAGTAGTCTTAGATAATTGTCTTAAAATTTCAGTTGAGACGTGCATAAGCTAGTTCAAATACCTATGATTAGTAGTCAAAGGCATAATTTGAGTATGCTTTTCGATCTTTAAACATGTATAGAGAACTAAACTGCATAGAGAATTAAATTATAGATCTTTAGATGTATAGTTTCTAATTGCTGTTTTGTTGGTATGGTTCCTGATTTTATGTAAATTATGAACACTTGGAGCAGGGGCCTATGTTTGCCTTGATTGGAGCTTGGCTTAGTTACCAATTCCAAAACAAAGATGTGGTTGCAAAGGATGTTTCAGACAACATGTTTTTTAAGGCAGTAATTGCAGCTGTCATCAGTTCCATTTTAAGTAATATTGGTCCAATTGATGAATGGTAAGTTCAAAAGTCTCTCATTTCAAACATAAAAAAAAAACCGAGTCTTATTTCTCCTCTCTCTAGAAGATATTACGACTCAAGTTGGAAAATAAAACTTACAACTTTTAATAGTGAGTTTGAGATGACTTTTAAAAATGGTGCTTTTAAGTGAAACACTTTTAGAAGAAGCACTAATTAAGTGTTTTTAAGTATATTGAAGAGGTTTAAAATCACTTTTGAGTTAGTAGCCAAACAATATCAAAGATTTAAAAGATTTTTTTCTTAGAGCTAAAATCACTTTTCATCATTCCAAAAGTCATCCTCATCCCTAACTTTCTCGAGAGAAGTTGCTAATACATTGATCATTCGAGTTATGCTCATGCTTACCGGAGTGCAATATAACAGGGTAAATGTAAAATAAAATCCAAAATCTTTATGTAATTTTGGTTTGTTTGGTGTTGTAGGACTCACACAGGAGCAGCTTTCAGTGGGATGCTATATGGGTTTTTAACATCCCCAGTTATGGAGGTGAATAATGCTGCTGTTTCTTCTTCTTCAAGGAGAGGCCAAGAAAAGGGAATCAAATTTGTTAAGAAGTATGCTGATCCTTGCAAATCATTTGCTTTTTTTGCTTTCTTCATCATGGGATTCACAAGTTTGCTCTTCTTTATTCAACCTCCTCCCACCACATTGCCATTATATGACTTTTGATTGGGTTTTAGATCAAGTTTGGGTCAGGCTTGAGAGGAAATTTCTACCCAAATATTGTCAAAATATTAAGTTTATAGTGTGGTTTGCAACTTGCAAGTGCAATGATGTACAGTAAAATATAATTATGCCTCCAAGCTTACCTTACAATTTTGTCTTCCACTTGCATTAGGACTCTCAAAATCCTATTTTAAATCATATTATGATACTTAAAACTCTTTCTATTATAGTCCTCAATATACTTTGTATAAAAGGTCCATTTAGTCCTTGTAATAAATGTTTCATTAATCAATATAC

mRNA sequence

AATCTTCTTAACTCCAAAAACCACTTAGGTGTTTAATCACCTTCAAAAATCCTCCCTTTCGATCATTAGCTCAAATATACACTCACGTCCAAAATGATGGCTGTTGTAATTCCCACCCTTTGCCACACACGACACCTTAACAACTCGATCAATCCAACCAAACAAATCATTTTTGGATACAATAAGAGACCTACGACTTGCAAATACGATCTTGTTTTAAAGAATTTGCCAATTTTTGAGTGTCGTAAACATTGGCAAGAGATTTCAAATAACGATTCATTTGTTGTTATGGTCTCGGCAAGAACGAAAAAGTCGAGAAGATGGTCGAAGGCGCTATTGCAAAGTGATGAAAGGCATGTGAGATGCAGTGGTTTGGATTTTGAATCACAAGTTTTCAAAAAACTATGCCATGTTTGGGAGTGTAGTACTAATGAGAAACAGTTGAGATCATTGGGTTCTTATTTTGGAAGGCTTCAAGGTGGTGGAAATAAGAAAAAAATGGATTCTTTGAAGAAGATAGAGGTGCTTGATACTGGTCAGTTTAAAGCAAAGAAGGAGCTTGAGTTGCTTGATGCTTATTTTGAAAAAGTTGATAAAGATCTAGATACGCACAACCATGCTCGGTCTAATACTTTTGGAGAACAACATGAAGGTAAATCAGTCTTCACATCATTCCCTTTTGAAGATTGCGCTAGAGATGACGAGATACAGATACGACACGAGAAACCGAATGATATGATGCATAGAAGCATTCCAAAAACAACTCAAATGCAAGAAGATGAAGTTTGTGATCTCTACTTGATAAGTACTCTGGTTTCTGTAAACATAGCAGTTTTCTTGTTTGAAGTAGCAAGTCCAGTAAAAAACTCAGACCTGCCACTCTTTTCACTCCCTTCTCTATATGGAGCAAAGATAAATGACCTGATTCTAGTAGGAGAATGGTGGAGGCTTGTGACACCTATGTTTCTGCATTCAGGAGTTCTTCATGTTGCTCTCAGTTGTTGGACACTTCTTACATTTGGCCGTCAAGTTTGTAGAGACTATGGTCCCTTCACATTTTTCTTGATCTATGTCCTTGGAGGAGTTTCTGGCAACCTCACAAGCTTTCTTCATACTCCCGAGCCAACTGTCGGTGGCACGGGGCCTATGTTTGCCTTGATTGGAGCTTGGCTTAGTTACCAATTCCAAAACAAAGATGTGGTTGCAAAGGATGTTTCAGACAACATGTTTTTTAAGGCAGTAATTGCAGCTGTCATCAGTTCCATTTTAAGTAATATTGGTCCAATTGATGAATGGACTCACACAGGAGCAGCTTTCAGTGGGATGCTATATGGGTTTTTAACATCCCCAGTTATGGAGGTGAATAATGCTGCTGTTTCTTCTTCTTCAAGGAGAGGCCAAGAAAAGGGAATCAAATTTGTTAAGAAGTATGCTGATCCTTGCAAATCATTTGCTTTTTTTGCTTTCTTCATCATGGGATTCACAAGTTTGCTCTTCTTTATTCAACCTCCTCCCACCACATTGCCATTATATGACTTTTGATTGGGTTTTAGATCAAGTTTGGGTCAGGCTTGAGAGGAAATTTCTACCCAAATATTGTCAAAATATTAAGTTTATAGTGTGGTTTGCAACTTGCAAGTGCAATGATGTACAGTAAAATATAATTATGCCTCCAAGCTTACCTTACAATTTTGTCTTCCACTTGCATTAGGACTCTCAAAATCCTATTTTAAATCATATTATGATACTTAAAACTCTTTCTATTATAGTCCTCAATATACTTTGTATAAAAGGTCCATTTAGTCCTTGTAATAAATGTTTCATTAATCAATATAC

Coding sequence (CDS)

ATGATGGCTGTTGTAATTCCCACCCTTTGCCACACACGACACCTTAACAACTCGATCAATCCAACCAAACAAATCATTTTTGGATACAATAAGAGACCTACGACTTGCAAATACGATCTTGTTTTAAAGAATTTGCCAATTTTTGAGTGTCGTAAACATTGGCAAGAGATTTCAAATAACGATTCATTTGTTGTTATGGTCTCGGCAAGAACGAAAAAGTCGAGAAGATGGTCGAAGGCGCTATTGCAAAGTGATGAAAGGCATGTGAGATGCAGTGGTTTGGATTTTGAATCACAAGTTTTCAAAAAACTATGCCATGTTTGGGAGTGTAGTACTAATGAGAAACAGTTGAGATCATTGGGTTCTTATTTTGGAAGGCTTCAAGGTGGTGGAAATAAGAAAAAAATGGATTCTTTGAAGAAGATAGAGGTGCTTGATACTGGTCAGTTTAAAGCAAAGAAGGAGCTTGAGTTGCTTGATGCTTATTTTGAAAAAGTTGATAAAGATCTAGATACGCACAACCATGCTCGGTCTAATACTTTTGGAGAACAACATGAAGGTAAATCAGTCTTCACATCATTCCCTTTTGAAGATTGCGCTAGAGATGACGAGATACAGATACGACACGAGAAACCGAATGATATGATGCATAGAAGCATTCCAAAAACAACTCAAATGCAAGAAGATGAAGTTTGTGATCTCTACTTGATAAGTACTCTGGTTTCTGTAAACATAGCAGTTTTCTTGTTTGAAGTAGCAAGTCCAGTAAAAAACTCAGACCTGCCACTCTTTTCACTCCCTTCTCTATATGGAGCAAAGATAAATGACCTGATTCTAGTAGGAGAATGGTGGAGGCTTGTGACACCTATGTTTCTGCATTCAGGAGTTCTTCATGTTGCTCTCAGTTGTTGGACACTTCTTACATTTGGCCGTCAAGTTTGTAGAGACTATGGTCCCTTCACATTTTTCTTGATCTATGTCCTTGGAGGAGTTTCTGGCAACCTCACAAGCTTTCTTCATACTCCCGAGCCAACTGTCGGTGGCACGGGGCCTATGTTTGCCTTGATTGGAGCTTGGCTTAGTTACCAATTCCAAAACAAAGATGTGGTTGCAAAGGATGTTTCAGACAACATGTTTTTTAAGGCAGTAATTGCAGCTGTCATCAGTTCCATTTTAAGTAATATTGGTCCAATTGATGAATGGACTCACACAGGAGCAGCTTTCAGTGGGATGCTATATGGGTTTTTAACATCCCCAGTTATGGAGGTGAATAATGCTGCTGTTTCTTCTTCTTCAAGGAGAGGCCAAGAAAAGGGAATCAAATTTGTTAAGAAGTATGCTGATCCTTGCAAATCATTTGCTTTTTTTGCTTTCTTCATCATGGGATTCACAAGTTTGCTCTTCTTTATTCAACCTCCTCCCACCACATTGCCATTATATGACTTTTGA

Protein sequence

MMAVVIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVLKNLPIFECRKHWQEISNNDSFVVMVSARTKKSRRWSKALLQSDERHVRCSGLDFESQVFKKLCHVWECSTNEKQLRSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDKDLDTHNHARSNTFGEQHEGKSVFTSFPFEDCARDDEIQIRHEKPNDMMHRSIPKTTQMQEDEVCDLYLISTLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSLLFFIQPPPTTLPLYDF
Homology
BLAST of Tan0015974 vs. ExPASy Swiss-Prot
Match: Q9FFX0 (RHOMBOID-like protein 9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL9 PE=2 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 2.3e-79
Identity = 173/387 (44.70%), Postives = 231/387 (59.69%), Query Frame = 0

Query: 102 KKLCHVWECSTN---EKQLRSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELEL 161
           ++LC V   S N   +++L+ L SYFG+LQ    K  + +   I+     +    +EL+ 
Sbjct: 62  RRLCLVRASSENKITKQRLKLLDSYFGKLQNDDEKPSISTGDDID--RKAELNVNEELDS 121

Query: 162 LDAYFEKVDKDLDTHNHARSNTFGEQHEGKSVFTSFPFEDCARDDEIQIRHEKPNDMMHR 221
           L AY +K+ KD  +     S     + EG SV +        R   I+  +  P      
Sbjct: 122 LSAYLDKLQKDAKSKGLVSSTLDVVKSEGGSVASKL------RKTGIE-NNNSPFQQF-- 181

Query: 222 SIPKTTQMQEDEVCDLYLISTLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLI 281
                 + Q ++  + Y +S L S+N+ V LFE A+PV+N+++ L SLP LYGAKINDLI
Sbjct: 182 ----DDEDQAEDTLNFYAVSILASINVGVCLFEAAAPVRNNNMGLLSLPLLYGAKINDLI 241

Query: 282 LVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSF 341
           L GEWWRLVTPMFLHSG+ HVALS W LLTFG +VCRDYG FTF LIY+LGGVSGN  SF
Sbjct: 242 LAGEWWRLVTPMFLHSGIPHVALSSWALLTFGPKVCRDYGLFTFCLIYILGGVSGNFMSF 301

Query: 342 LHTPEPTVGGTGPMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPI 401
           LHT +PTVGGTGP FALIGAWL  Q QNK+++  +  +++F KA+I      ILS+ GPI
Sbjct: 302 LHTADPTVGGTGPAFALIGAWLVDQNQNKEMIKSNEYEDLFQKAIIMTGFGLILSHFGPI 361

Query: 402 DEWTHTGAAFSGMLYGFLTSPVMEVNNAAVSSSSRRGQEKGIKFV----KKYADPCKSFA 461
           D+WT+ GA  +G++YGF T PV+++     S  S R  ++GI  V    +  ADPCKSF 
Sbjct: 362 DDWTNLGALIAGIVYGFFTCPVLQLG----SGGSER--QEGIVTVGPEKQNSADPCKSFL 421

Query: 462 FFAFFIMGFTSLLFFIQPPPTTLPLYD 482
            F  F+    + L  I   P   P YD
Sbjct: 422 LFTIFVAVIVTSLLLIGDGPLDFPTYD 427

BLAST of Tan0015974 vs. ExPASy Swiss-Prot
Match: P54493 (Rhomboid protease GluP OS=Bacillus subtilis (strain 168) OX=224308 GN=gluP PE=1 SV=2)

HSP 1 Score: 103.6 bits (257), Expect = 6.4e-21
Identity = 71/247 (28.74%), Postives = 118/247 (47.77%), Query Frame = 0

Query: 188 KSVFTSFPFEDCARD----DEIQIRHEKPNDMM----HRSIPKTTQMQEDEVCDLYLIST 247
           ++VF SF  EDC+ +    +  Q+  E+   ++     +   +    Q  +    YL   
Sbjct: 126 QAVFPSFRTEDCSEEHASFENAQMARERFLSLVLKQEEQRKTEAAVFQNGKPTFTYLFIA 185

Query: 248 LVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSGVLHV 307
           L    +  FL E+     N++  +      +GAK N LI  GEWWRL+TP+ LH G+ H+
Sbjct: 186 LQI--LMFFLLEINGGSTNTETLV-----AFGAKENSLIAQGEWWRLLTPIVLHIGIAHL 245

Query: 308 ALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFALIGAW 367
           A +   L + G  V R YG   F LIY+  G++G++ SF+ +P P+ G +G +F  +GA 
Sbjct: 246 AFNTLALWSVGTAVERMYGSGRFLLIYLAAGITGSIASFVFSPYPSAGASGAIFGCLGAL 305

Query: 368 LSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSP 427
           L     N+ +  + +  N+    +I       +SN   ID   H G    G++ GF  + 
Sbjct: 306 LYVALSNRKMFLRTIGTNIIVIIIINLGFGFAVSN---IDNSGHIG----GLIGGFFAAA 358

BLAST of Tan0015974 vs. ExPASy Swiss-Prot
Match: F4ICF4 (RHOMBOID-like protein 10, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL10 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.0e-14
Identity = 52/189 (27.51%), Postives = 91/189 (48.15%), Query Frame = 0

Query: 238 STLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSGVL 297
           + L+++N+ +++ ++AS  K            +GAKIN LI  G+ WRL T   LH+  +
Sbjct: 131 NVLLAINVIMYIAQIASDGK---------VLTWGAKINSLIERGQLWRLATASVLHANPM 190

Query: 298 HVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVS-------GNLTSFLHTPEPTVGGTG 357
           H+ ++C++L + G       GP  F  +Y+   V+       G+  S+     P+VG +G
Sbjct: 191 HLMINCYSLNSIGPTAESLGGPKRFLAVYLTSAVAKPILRVLGSAMSYWFNKAPSVGASG 250

Query: 358 PMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSG 417
            +F L+G+   +  ++K +V     D M    +IA  ++  L +   ID W H G    G
Sbjct: 251 AIFGLVGSVAVFVIRHKQMVRGGNEDLMQIAQIIALNMAMGLMS-RRIDNWGHIGGLLGG 309

Query: 418 MLYGFLTSP 420
               +L  P
Sbjct: 311 TAMTWLLGP 309

BLAST of Tan0015974 vs. ExPASy Swiss-Prot
Match: Q8VZ48 (RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.9e-12
Identity = 48/181 (26.52%), Postives = 92/181 (50.83%), Query Frame = 0

Query: 282 EWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHT 341
           E WRL+T M+LH+G++H+ ++ + ++ FG ++ + +G     LIY++ G  G++ S L  
Sbjct: 96  EKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRIGLIYLISGFGGSILSALFL 155

Query: 342 PEP-TVGGTGPMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDE 401
            +  +VG +G +  L+GA LS    N  +    +   + F  +IA  I+  +  +  +D 
Sbjct: 156 QKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLSFLFIIA--INLAIGLLPWVDN 215

Query: 402 WTHTGAAFSGMLYGFLTSPVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFI 461
           + H G    G+L GF    ++ +   +     R   + G +   KY +PC+   FF   +
Sbjct: 216 FAHIG----GLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSKY-NPCQYVLFFVAAV 269

BLAST of Tan0015974 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 7.1e-12
Identity = 43/153 (28.10%), Postives = 80/153 (52.29%), Query Frame = 0

Query: 265 SLPSLYGAKINDLILVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCRDYGPFTFFL 324
           +L ++ G  +  ++   E WRL++  +LH GV+H+ ++  TLL  G ++ R++G     L
Sbjct: 121 TLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGFIRIGL 180

Query: 325 IYVLGGVSGNLTSFLH-TPEPTVGGTGPMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAV 384
           +Y++ G  G++ S L      +VG +G +F L+G  LS  F N  + +  V   +    +
Sbjct: 181 LYLISGFGGSILSALFLRSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTIVTLVLI 240

Query: 385 IAAVISSILSNIGPIDEWTHTGAAFSGMLYGFL 417
           +A  +   L  +  +D + H G   +G L GF+
Sbjct: 241 VAVNLG--LGVLPGVDNFAHIGGFATGFLLGFV 271

BLAST of Tan0015974 vs. NCBI nr
Match: XP_038883181.1 (RHOMBOID-like protein 9, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 687.2 bits (1772), Expect = 1.0e-193
Identity = 358/477 (75.05%), Postives = 402/477 (84.28%), Query Frame = 0

Query: 5   VIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVLK-NLPIFECRK--HWQEISNND 64
           V+PT+   R+ N+SINPT+ I     KR + CK D+V K NLPIFE  K  HW EI NND
Sbjct: 3   VVPTIFQIRNFNDSINPTQIISKPNYKRASICKCDVVSKNNLPIFEYSKNRHW-EIQNND 62

Query: 65  SFVVMVSARTKKSRRWSKALLQSDERHVRC-SGLDFESQVFKKLCHVWECSTNEKQLRSL 124
           S V+ ++AR  KSRR  KA+     +HVRC SGLDFESQV KKL  VWECSTNEKQLRSL
Sbjct: 63  SSVITIAARATKSRRRLKAMFL---QHVRCSSGLDFESQVLKKLSQVWECSTNEKQLRSL 122

Query: 125 GSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDKDLDTHNHARSNT 184
           GSYFGRLQG GNKK +DSL K +VLDTGQFKAKKELELLDAYF+KVD+DL  H+    ++
Sbjct: 123 GSYFGRLQGVGNKKNLDSLNKRKVLDTGQFKAKKELELLDAYFQKVDEDLQNHH---LSS 182

Query: 185 FGEQHEGKSVFTSFPFEDCARDD-EIQIRHEKPNDMMHRSIPKTTQMQEDEVCDLYLIST 244
           F E  E K VFTS PFED  +DD E+ IRH K N M+HRS+PKT Q+QE++VCDLYLIS 
Sbjct: 183 FDELQEDKLVFTSSPFEDRNKDDNEMHIRHVKLNGMIHRSMPKTAQLQEEDVCDLYLISA 242

Query: 245 LVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSGVLHV 304
           LVS+NIAV LFE+ASPVKNS+L LFSLPSLYGAKIN+LILVGEWWRLVTPMFLHSGVLHV
Sbjct: 243 LVSINIAVLLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHV 302

Query: 305 ALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFALIGAW 364
           ALSCWTL+TFGRQVCR+YGPFTFFLIYVLGGVSGNLTSFLHTP+PTVGGTGP+FA+IGAW
Sbjct: 303 ALSCWTLVTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAW 362

Query: 365 LSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSP 424
           LSYQFQ+KDVV KDVSDNMF KA+IAAVISSILSNIGPIDEW+HTGAAFSGMLYGFLTSP
Sbjct: 363 LSYQFQDKDVVTKDVSDNMFLKAIIAAVISSILSNIGPIDEWSHTGAAFSGMLYGFLTSP 422

Query: 425 VMEVNNAA---VSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSLLFFIQPP 474
           ++EVN+AA    SSSSR+GQ KGIK V+KYA PCKS A FA FIMGFTSL+FFIQPP
Sbjct: 423 IVEVNDAASSSSSSSSRKGQHKGIKLVRKYAHPCKSLAVFALFIMGFTSLVFFIQPP 472

BLAST of Tan0015974 vs. NCBI nr
Match: XP_038883174.1 (RHOMBOID-like protein 9, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 682.9 bits (1761), Expect = 1.9e-192
Identity = 359/478 (75.10%), Postives = 401/478 (83.89%), Query Frame = 0

Query: 5   VIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVLK-NLPIFECRK--HWQEISNND 64
           V+PT+   R+ N+SINPT+ I     KR + CK D+V K NLPIFE  K  HW EI NND
Sbjct: 3   VVPTIFQIRNFNDSINPTQIISKPNYKRASICKCDVVSKNNLPIFEYSKNRHW-EIQNND 62

Query: 65  SFVVMVSARTKKSRRWSKALLQSDERHVRC-SGLDFESQVFKKLCHVWECSTNEKQLRSL 124
           S V+ ++AR  KSRR  KA+     +HVRC SGLDFESQV KKL  VWECSTNEKQLRSL
Sbjct: 63  SSVITIAARATKSRRRLKAMFL---QHVRCSSGLDFESQVLKKLSQVWECSTNEKQLRSL 122

Query: 125 GSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDK-DLDTHNHARSN 184
           GSYFGRLQG GNKK +DSL K +VLDTGQFKAKKELELLDAYF+KVD+   D  NH  S 
Sbjct: 123 GSYFGRLQGVGNKKNLDSLNKRKVLDTGQFKAKKELELLDAYFQKVDEAHTDLQNHHLS- 182

Query: 185 TFGEQHEGKSVFTSFPFEDCARDD-EIQIRHEKPNDMMHRSIPKTTQMQEDEVCDLYLIS 244
           +F E  E K VFTS PFED  +DD E+ IRH K N M+HRS+PKT Q+QE++VCDLYLIS
Sbjct: 183 SFDELQEDKLVFTSSPFEDRNKDDNEMHIRHVKLNGMIHRSMPKTAQLQEEDVCDLYLIS 242

Query: 245 TLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSGVLH 304
            LVS+NIAV LFE+ASPVKNS+L LFSLPSLYGAKIN+LILVGEWWRLVTPMFLHSGVLH
Sbjct: 243 ALVSINIAVLLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLH 302

Query: 305 VALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFALIGA 364
           VALSCWTL+TFGRQVCR+YGPFTFFLIYVLGGVSGNLTSFLHTP+PTVGGTGP+FA+IGA
Sbjct: 303 VALSCWTLVTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGA 362

Query: 365 WLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTS 424
           WLSYQFQ+KDVV KDVSDNMF KA+IAAVISSILSNIGPIDEW+HTGAAFSGMLYGFLTS
Sbjct: 363 WLSYQFQDKDVVTKDVSDNMFLKAIIAAVISSILSNIGPIDEWSHTGAAFSGMLYGFLTS 422

Query: 425 PVMEVNNAA---VSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSLLFFIQPP 474
           P++EVN+AA    SSSSR+GQ KGIK V+KYA PCKS A FA FIMGFTSL+FFIQPP
Sbjct: 423 PIVEVNDAASSSSSSSSRKGQHKGIKLVRKYAHPCKSLAVFALFIMGFTSLVFFIQPP 475

BLAST of Tan0015974 vs. NCBI nr
Match: XP_004137292.3 (RHOMBOID-like protein 9, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 679.1 bits (1751), Expect = 2.8e-191
Identity = 350/478 (73.22%), Postives = 398/478 (83.26%), Query Frame = 0

Query: 2   MAVVIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVLK--NLPIF--ECRKHWQEI 61
           M  V+PT    R+ N +I+         NKR   CK+D+V+   NLP F    + H  EI
Sbjct: 36  MMAVVPTFFQIRNFNETISKP-------NKRVCVCKWDVVVSKDNLPTFVSYSKHHTWEI 95

Query: 62  SNNDSFVVMVSARTKKSRRWSKALLQSDERHVRCS-GLDFE-SQVFKKLCHVWECSTNEK 121
            +NDS VV +S R +KSRR  KALL     HVRCS GLDFE S+V +KL  VWECSTNEK
Sbjct: 96  HDNDSSVVTISTRARKSRRGLKALL----HHVRCSNGLDFEHSKVSEKLSQVWECSTNEK 155

Query: 122 QLRSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDKDLDTHNH 181
           QLRSLGSYFGRLQG G++K +DSLKK++VLDTGQFKAKKEL+LLDAYF+KVDKD   H H
Sbjct: 156 QLRSLGSYFGRLQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTGLHKH 215

Query: 182 ARSNTFGEQHEGKSVFTSFPFEDCARDDEIQIRHEKPNDMMHRSIPKTTQMQEDEVCDLY 241
             S +F EQ EGK VFT+ P ED  +DDE+QIRH K + M+H+S+P  TQ+QE+EVCDLY
Sbjct: 216 PLS-SFDEQEEGKLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLY 275

Query: 242 LISTLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSG 301
           LIS LVS+NIAVFLFEVASPVKNS+L LFSLPSLYGAKIN+LILVGEWWRLVTPMFLHSG
Sbjct: 276 LISALVSINIAVFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSG 335

Query: 302 VLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFAL 361
           VLHVALSCWTLLTFGRQVCR+YGPFTFFLIYVLGGVSGNLTSFLHTP+PTVGGTGP+FA+
Sbjct: 336 VLHVALSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAM 395

Query: 362 IGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYGF 421
           IGAWLSYQFQNKDV+ KDVSD MF KA++AAVISSILSNIGPIDEWTHTGAAFSGMLYGF
Sbjct: 396 IGAWLSYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGF 455

Query: 422 LTSPVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSLLFFIQPP 474
           LTSP++EVN+A+ SSSSRRGQEKGIK V+KYA+PC+S AFF  FIMGF SL+FFIQPP
Sbjct: 456 LTSPIVEVNDASSSSSSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPP 501

BLAST of Tan0015974 vs. NCBI nr
Match: XP_011653344.2 (RHOMBOID-like protein 9, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 674.5 bits (1739), Expect = 6.9e-190
Identity = 350/479 (73.07%), Postives = 398/479 (83.09%), Query Frame = 0

Query: 2   MAVVIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVLK--NLPIF--ECRKHWQEI 61
           M  V+PT    R+ N +I+         NKR   CK+D+V+   NLP F    + H  EI
Sbjct: 36  MMAVVPTFFQIRNFNETISKP-------NKRVCVCKWDVVVSKDNLPTFVSYSKHHTWEI 95

Query: 62  SNNDSFVVMVSARTKKSRRWSKALLQSDERHVRCS-GLDFE-SQVFKKLCHVWECSTNEK 121
            +NDS VV +S R +KSRR  KALL     HVRCS GLDFE S+V +KL  VWECSTNEK
Sbjct: 96  HDNDSSVVTISTRARKSRRGLKALL----HHVRCSNGLDFEHSKVSEKLSQVWECSTNEK 155

Query: 122 QLRSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDK-DLDTHN 181
           QLRSLGSYFGRLQG G++K +DSLKK++VLDTGQFKAKKEL+LLDAYF+KVDK D   H 
Sbjct: 156 QLRSLGSYFGRLQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKADTGLHK 215

Query: 182 HARSNTFGEQHEGKSVFTSFPFEDCARDDEIQIRHEKPNDMMHRSIPKTTQMQEDEVCDL 241
           H  S +F EQ EGK VFT+ P ED  +DDE+QIRH K + M+H+S+P  TQ+QE+EVCDL
Sbjct: 216 HPLS-SFDEQEEGKLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDL 275

Query: 242 YLISTLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHS 301
           YLIS LVS+NIAVFLFEVASPVKNS+L LFSLPSLYGAKIN+LILVGEWWRLVTPMFLHS
Sbjct: 276 YLISALVSINIAVFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHS 335

Query: 302 GVLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFA 361
           GVLHVALSCWTLLTFGRQVCR+YGPFTFFLIYVLGGVSGNLTSFLHTP+PTVGGTGP+FA
Sbjct: 336 GVLHVALSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFA 395

Query: 362 LIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYG 421
           +IGAWLSYQFQNKDV+ KDVSD MF KA++AAVISSILSNIGPIDEWTHTGAAFSGMLYG
Sbjct: 396 MIGAWLSYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYG 455

Query: 422 FLTSPVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSLLFFIQPP 474
           FLTSP++EVN+A+ SSSSRRGQEKGIK V+KYA+PC+S AFF  FIMGF SL+FFIQPP
Sbjct: 456 FLTSPIVEVNDASSSSSSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPP 502

BLAST of Tan0015974 vs. NCBI nr
Match: XP_011653345.2 (RHOMBOID-like protein 9, chloroplastic isoform X4 [Cucumis sativus])

HSP 1 Score: 674.1 bits (1738), Expect = 9.0e-190
Identity = 350/478 (73.22%), Postives = 398/478 (83.26%), Query Frame = 0

Query: 2   MAVVIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVLK--NLPIF--ECRKHWQEI 61
           M  V+PT    R+ N +I+         NKR   CK+D+V+   NLP F    + H  EI
Sbjct: 36  MMAVVPTFFQIRNFNETISKP-------NKRVCVCKWDVVVSKDNLPTFVSYSKHHTWEI 95

Query: 62  SNNDSFVVMVSARTKKSRRWSKALLQSDERHVRCS-GLDFE-SQVFKKLCHVWECSTNEK 121
            +NDS VV +S R +KSRR  KALL     HVRCS GLDFE S+V +KL  VWECSTNEK
Sbjct: 96  HDNDSSVVTISTRARKSRRGLKALL----HHVRCSNGLDFEHSKVSEKLSQVWECSTNEK 155

Query: 122 QLRSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDKDLDTHNH 181
           QLRSLGSYFGRLQG G++K +DSLKK++VLDTGQFKAKKEL+LLDAYF+KVDK L  H H
Sbjct: 156 QLRSLGSYFGRLQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKGL--HKH 215

Query: 182 ARSNTFGEQHEGKSVFTSFPFEDCARDDEIQIRHEKPNDMMHRSIPKTTQMQEDEVCDLY 241
             S +F EQ EGK VFT+ P ED  +DDE+QIRH K + M+H+S+P  TQ+QE+EVCDLY
Sbjct: 216 PLS-SFDEQEEGKLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLY 275

Query: 242 LISTLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSG 301
           LIS LVS+NIAVFLFEVASPVKNS+L LFSLPSLYGAKIN+LILVGEWWRLVTPMFLHSG
Sbjct: 276 LISALVSINIAVFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSG 335

Query: 302 VLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFAL 361
           VLHVALSCWTLLTFGRQVCR+YGPFTFFLIYVLGGVSGNLTSFLHTP+PTVGGTGP+FA+
Sbjct: 336 VLHVALSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAM 395

Query: 362 IGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYGF 421
           IGAWLSYQFQNKDV+ KDVSD MF KA++AAVISSILSNIGPIDEWTHTGAAFSGMLYGF
Sbjct: 396 IGAWLSYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGF 455

Query: 422 LTSPVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSLLFFIQPP 474
           LTSP++EVN+A+ SSSSRRGQEKGIK V+KYA+PC+S AFF  FIMGF SL+FFIQPP
Sbjct: 456 LTSPIVEVNDASSSSSSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPP 499

BLAST of Tan0015974 vs. ExPASy TrEMBL
Match: A0A0A0L0I7 (Rhomboid domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G094530 PE=3 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 9.7e-190
Identity = 349/475 (73.47%), Postives = 397/475 (83.58%), Query Frame = 0

Query: 5   VIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVLK--NLPIF--ECRKHWQEISNN 64
           V+PT    R+ N +I+         NKR   CK+D+V+   NLP F    + H  EI +N
Sbjct: 3   VVPTFFQIRNFNETISKP-------NKRVCVCKWDVVVSKDNLPTFVSYSKHHTWEIHDN 62

Query: 65  DSFVVMVSARTKKSRRWSKALLQSDERHVRCS-GLDFE-SQVFKKLCHVWECSTNEKQLR 124
           DS VV +S R +KSRR  KALL     HVRCS GLDFE S+V +KL  VWECSTNEKQLR
Sbjct: 63  DSSVVTISTRARKSRRGLKALL----HHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLR 122

Query: 125 SLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDKDLDTHNHARS 184
           SLGSYFGRLQG G++K +DSLKK++VLDTGQFKAKKEL+LLDAYF+KVDK L  H H  S
Sbjct: 123 SLGSYFGRLQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKGL--HKHPLS 182

Query: 185 NTFGEQHEGKSVFTSFPFEDCARDDEIQIRHEKPNDMMHRSIPKTTQMQEDEVCDLYLIS 244
            +F EQ EGK VFT+ P ED  +DDE+QIRH K + M+H+S+P  TQ+QE+EVCDLYLIS
Sbjct: 183 -SFDEQEEGKLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLIS 242

Query: 245 TLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSGVLH 304
            LVS+NIAVFLFEVASPVKNS+L LFSLPSLYGAKIN+LILVGEWWRLVTPMFLHSGVLH
Sbjct: 243 ALVSINIAVFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLH 302

Query: 305 VALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFALIGA 364
           VALSCWTLLTFGRQVCR+YGPFTFFLIYVLGGVSGNLTSFLHTP+PTVGGTGP+FA+IGA
Sbjct: 303 VALSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGA 362

Query: 365 WLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTS 424
           WLSYQFQNKDV+ KDVSD MF KA++AAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTS
Sbjct: 363 WLSYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTS 422

Query: 425 PVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSLLFFIQPP 474
           P++EVN+A+ SSSSRRGQEKGIK V+KYA+PC+S AFF  FIMGF SL+FFIQPP
Sbjct: 423 PIVEVNDASSSSSSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPP 463

BLAST of Tan0015974 vs. ExPASy TrEMBL
Match: A0A1S3C0U5 (RHOMBOID-like protein 9, chloroplastic isoform X5 OS=Cucumis melo OX=3656 GN=LOC103495725 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 3.1e-188
Identity = 350/477 (73.38%), Postives = 397/477 (83.23%), Query Frame = 0

Query: 4   VVIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVL--KNLPIF--ECRKHWQEISN 63
           + +PT    R+ N+ +NPT Q I   NKR   CK D+V+   NLPI     + H  EI N
Sbjct: 1   MAVPTFFQIRNFNDLLNPT-QTISKPNKRVCICKCDVVVSKNNLPILVTYSKHHNWEIHN 60

Query: 64  NDSFVVMVSARTKKSRRWSKALLQSDERHVRCS-GLDFE-SQVFKKLCHVWECSTNEKQL 123
           NDS VV +S R +KS    KALLQ    HVRCS GL+FE S+V +KL  VW+C+TNEKQL
Sbjct: 61  NDSSVVKISTRARKSIGGLKALLQ----HVRCSNGLNFEYSKVLEKLSQVWKCTTNEKQL 120

Query: 124 RSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDKDLDTHNHAR 183
           RSLGSYFGRLQG G+KK +DSLKK++VLDTGQFKAKKEL+LLDAYF+KVDKD D H H  
Sbjct: 121 RSLGSYFGRLQGLGDKKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTDLHKHPL 180

Query: 184 SNTFGEQHEGKSVFTSFPFEDCARDDE-IQIRHEKPNDMMHRSIPKTTQMQEDEVCDLYL 243
           S +F +Q EGK VFT+ PFED  +DD+  QI+H K + M H+S+P TTQ+QE+EVCDLYL
Sbjct: 181 S-SFDDQQEGKLVFTTSPFEDYVKDDDKSQIQHVKLSGMTHKSMPDTTQLQEEEVCDLYL 240

Query: 244 ISTLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSGV 303
           IS LVS+NIAVFLFE+ASPVKNS+L LFSLPSLYGAKIN+LILVGEWWRLVTPMFLHSGV
Sbjct: 241 ISALVSINIAVFLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGV 300

Query: 304 LHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFALI 363
           LHVALSCWTLLTFGRQVCR YGPFTFFLIYVLGGVSGNLTSFLHTP PTVGGTGP+FA+I
Sbjct: 301 LHVALSCWTLLTFGRQVCRKYGPFTFFLIYVLGGVSGNLTSFLHTPNPTVGGTGPVFAMI 360

Query: 364 GAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYGFL 423
           GAWLSYQFQNKDVV KDVSD MF KA++AAVISSILSNIGPIDEWTHTGAAFSGMLYGFL
Sbjct: 361 GAWLSYQFQNKDVVTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFL 420

Query: 424 TSPVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSLLFFIQPP 474
           TSP+ +VN+A  SSSSRRGQEKGIK V+KYA+PCKSFAFF  FIMGF SL+FF+QPP
Sbjct: 421 TSPLGKVNDA--SSSSRRGQEKGIKVVRKYANPCKSFAFFVLFIMGFISLVFFVQPP 469

BLAST of Tan0015974 vs. ExPASy TrEMBL
Match: A0A1S3C2H9 (RHOMBOID-like protein 9, chloroplastic isoform X4 OS=Cucumis melo OX=3656 GN=LOC103495725 PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 7.7e-187
Identity = 350/478 (73.22%), Postives = 397/478 (83.05%), Query Frame = 0

Query: 4   VVIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVL--KNLPIF--ECRKHWQEISN 63
           + +PT    R+ N+ +NPT Q I   NKR   CK D+V+   NLPI     + H  EI N
Sbjct: 1   MAVPTFFQIRNFNDLLNPT-QTISKPNKRVCICKCDVVVSKNNLPILVTYSKHHNWEIHN 60

Query: 64  NDSFVVMVSARTKKSRRWSKALLQSDERHVRCS-GLDFE-SQVFKKLCHVWECSTNEKQL 123
           NDS VV +S R +KS    KALLQ    HVRCS GL+FE S+V +KL  VW+C+TNEKQL
Sbjct: 61  NDSSVVKISTRARKSIGGLKALLQ----HVRCSNGLNFEYSKVLEKLSQVWKCTTNEKQL 120

Query: 124 RSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDK-DLDTHNHA 183
           RSLGSYFGRLQG G+KK +DSLKK++VLDTGQFKAKKEL+LLDAYF+KVDK D D H H 
Sbjct: 121 RSLGSYFGRLQGLGDKKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKADTDLHKHP 180

Query: 184 RSNTFGEQHEGKSVFTSFPFEDCARDDE-IQIRHEKPNDMMHRSIPKTTQMQEDEVCDLY 243
            S +F +Q EGK VFT+ PFED  +DD+  QI+H K + M H+S+P TTQ+QE+EVCDLY
Sbjct: 181 LS-SFDDQQEGKLVFTTSPFEDYVKDDDKSQIQHVKLSGMTHKSMPDTTQLQEEEVCDLY 240

Query: 244 LISTLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSG 303
           LIS LVS+NIAVFLFE+ASPVKNS+L LFSLPSLYGAKIN+LILVGEWWRLVTPMFLHSG
Sbjct: 241 LISALVSINIAVFLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSG 300

Query: 304 VLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFAL 363
           VLHVALSCWTLLTFGRQVCR YGPFTFFLIYVLGGVSGNLTSFLHTP PTVGGTGP+FA+
Sbjct: 301 VLHVALSCWTLLTFGRQVCRKYGPFTFFLIYVLGGVSGNLTSFLHTPNPTVGGTGPVFAM 360

Query: 364 IGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYGF 423
           IGAWLSYQFQNKDVV KDVSD MF KA++AAVISSILSNIGPIDEWTHTGAAFSGMLYGF
Sbjct: 361 IGAWLSYQFQNKDVVTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGF 420

Query: 424 LTSPVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSLLFFIQPP 474
           LTSP+ +VN+A  SSSSRRGQEKGIK V+KYA+PCKSFAFF  FIMGF SL+FF+QPP
Sbjct: 421 LTSPLGKVNDA--SSSSRRGQEKGIKVVRKYANPCKSFAFFVLFIMGFISLVFFVQPP 470

BLAST of Tan0015974 vs. ExPASy TrEMBL
Match: A0A1S4E1D5 (RHOMBOID-like protein 9, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495725 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.5e-185
Identity = 349/486 (71.81%), Postives = 397/486 (81.69%), Query Frame = 0

Query: 4   VVIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVL--KNLPIF--ECRKHWQEISN 63
           + +PT    R+ N+ +NPT Q I   NKR   CK D+V+   NLPI     + H  EI N
Sbjct: 1   MAVPTFFQIRNFNDLLNPT-QTISKPNKRVCICKCDVVVSKNNLPILVTYSKHHNWEIHN 60

Query: 64  NDSFVVMVSARTKKSRRWSKALLQSDERHVRCS-GLDFE-SQVFKKLCHVWECSTNEKQL 123
           NDS VV +S R +KS    KALLQ    HVRCS GL+FE S+V +KL  VW+C+TNEKQL
Sbjct: 61  NDSSVVKISTRARKSIGGLKALLQ----HVRCSNGLNFEYSKVLEKLSQVWKCTTNEKQL 120

Query: 124 RSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDKDLDTHNHAR 183
           RSLGSYFGRLQG G+KK +DSLKK++VLDTGQFKAKKEL+LLDAYF+KVDKD D H H  
Sbjct: 121 RSLGSYFGRLQGLGDKKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTDLHKHPL 180

Query: 184 SNTFGEQHEGKSVFTSFPFEDCARDDE-IQIRHEKPNDMMHRSIPKTTQMQEDEVCDLYL 243
           S +F +Q EGK VFT+ PFED  +DD+  QI+H K + M H+S+P TTQ+QE+EVCDLYL
Sbjct: 181 S-SFDDQQEGKLVFTTSPFEDYVKDDDKSQIQHVKLSGMTHKSMPDTTQLQEEEVCDLYL 240

Query: 244 I---------STLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLV 303
           +         S LVS+NIAVFLFE+ASPVKNS+L LFSLPSLYGAKIN+LILVGEWWRLV
Sbjct: 241 MLLLEIYICRSALVSINIAVFLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLV 300

Query: 304 TPMFLHSGVLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVG 363
           TPMFLHSGVLHVALSCWTLLTFGRQVCR YGPFTFFLIYVLGGVSGNLTSFLHTP PTVG
Sbjct: 301 TPMFLHSGVLHVALSCWTLLTFGRQVCRKYGPFTFFLIYVLGGVSGNLTSFLHTPNPTVG 360

Query: 364 GTGPMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAA 423
           GTGP+FA+IGAWLSYQFQNKDVV KDVSD MF KA++AAVISSILSNIGPIDEWTHTGAA
Sbjct: 361 GTGPVFAMIGAWLSYQFQNKDVVTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAA 420

Query: 424 FSGMLYGFLTSPVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSLL 474
           FSGMLYGFLTSP+ +VN+A  SSSSRRGQEKGIK V+KYA+PCKSFAFF  FIMGF SL+
Sbjct: 421 FSGMLYGFLTSPLGKVNDA--SSSSRRGQEKGIKVVRKYANPCKSFAFFVLFIMGFISLV 478

BLAST of Tan0015974 vs. ExPASy TrEMBL
Match: A0A1S4E0Q3 (RHOMBOID-like protein 9, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495725 PE=3 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 3.6e-184
Identity = 349/487 (71.66%), Postives = 397/487 (81.52%), Query Frame = 0

Query: 4   VVIPTLCHTRHLNNSINPTKQIIFGYNKRPTTCKYDLVL--KNLPIF--ECRKHWQEISN 63
           + +PT    R+ N+ +NPT Q I   NKR   CK D+V+   NLPI     + H  EI N
Sbjct: 1   MAVPTFFQIRNFNDLLNPT-QTISKPNKRVCICKCDVVVSKNNLPILVTYSKHHNWEIHN 60

Query: 64  NDSFVVMVSARTKKSRRWSKALLQSDERHVRCS-GLDFE-SQVFKKLCHVWECSTNEKQL 123
           NDS VV +S R +KS    KALLQ    HVRCS GL+FE S+V +KL  VW+C+TNEKQL
Sbjct: 61  NDSSVVKISTRARKSIGGLKALLQ----HVRCSNGLNFEYSKVLEKLSQVWKCTTNEKQL 120

Query: 124 RSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELELLDAYFEKVDK-DLDTHNHA 183
           RSLGSYFGRLQG G+KK +DSLKK++VLDTGQFKAKKEL+LLDAYF+KVDK D D H H 
Sbjct: 121 RSLGSYFGRLQGLGDKKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKADTDLHKHP 180

Query: 184 RSNTFGEQHEGKSVFTSFPFEDCARDDE-IQIRHEKPNDMMHRSIPKTTQMQEDEVCDLY 243
            S +F +Q EGK VFT+ PFED  +DD+  QI+H K + M H+S+P TTQ+QE+EVCDLY
Sbjct: 181 LS-SFDDQQEGKLVFTTSPFEDYVKDDDKSQIQHVKLSGMTHKSMPDTTQLQEEEVCDLY 240

Query: 244 LI---------STLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRL 303
           L+         S LVS+NIAVFLFE+ASPVKNS+L LFSLPSLYGAKIN+LILVGEWWRL
Sbjct: 241 LMLLLEIYICRSALVSINIAVFLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRL 300

Query: 304 VTPMFLHSGVLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTV 363
           VTPMFLHSGVLHVALSCWTLLTFGRQVCR YGPFTFFLIYVLGGVSGNLTSFLHTP PTV
Sbjct: 301 VTPMFLHSGVLHVALSCWTLLTFGRQVCRKYGPFTFFLIYVLGGVSGNLTSFLHTPNPTV 360

Query: 364 GGTGPMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGA 423
           GGTGP+FA+IGAWLSYQFQNKDVV KDVSD MF KA++AAVISSILSNIGPIDEWTHTGA
Sbjct: 361 GGTGPVFAMIGAWLSYQFQNKDVVTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGA 420

Query: 424 AFSGMLYGFLTSPVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFIMGFTSL 474
           AFSGMLYGFLTSP+ +VN+A  SSSSRRGQEKGIK V+KYA+PCKSFAFF  FIMGF SL
Sbjct: 421 AFSGMLYGFLTSPLGKVNDA--SSSSRRGQEKGIKVVRKYANPCKSFAFFVLFIMGFISL 479

BLAST of Tan0015974 vs. TAIR 10
Match: AT5G38510.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-80
Identity = 173/387 (44.70%), Postives = 231/387 (59.69%), Query Frame = 0

Query: 102 KKLCHVWECSTN---EKQLRSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELEL 161
           ++LC V   S N   +++L+ L SYFG+LQ    K  + +   I+     +    +EL+ 
Sbjct: 62  RRLCLVRASSENKITKQRLKLLDSYFGKLQNDDEKPSISTGDDID--RKAELNVNEELDS 121

Query: 162 LDAYFEKVDKDLDTHNHARSNTFGEQHEGKSVFTSFPFEDCARDDEIQIRHEKPNDMMHR 221
           L AY +K+ KD  +     S     + EG SV +        R   I+  +  P      
Sbjct: 122 LSAYLDKLQKDAKSKGLVSSTLDVVKSEGGSVASKL------RKTGIE-NNNSPFQQF-- 181

Query: 222 SIPKTTQMQEDEVCDLYLISTLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLI 281
                 + Q ++  + Y +S L S+N+ V LFE A+PV+N+++ L SLP LYGAKINDLI
Sbjct: 182 ----DDEDQAEDTLNFYAVSILASINVGVCLFEAAAPVRNNNMGLLSLPLLYGAKINDLI 241

Query: 282 LVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSF 341
           L GEWWRLVTPMFLHSG+ HVALS W LLTFG +VCRDYG FTF LIY+LGGVSGN  SF
Sbjct: 242 LAGEWWRLVTPMFLHSGIPHVALSSWALLTFGPKVCRDYGLFTFCLIYILGGVSGNFMSF 301

Query: 342 LHTPEPTVGGTGPMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPI 401
           LHT +PTVGGTGP FALIGAWL  Q QNK+++  +  +++F KA+I      ILS+ GPI
Sbjct: 302 LHTADPTVGGTGPAFALIGAWLVDQNQNKEMIKSNEYEDLFQKAIIMTGFGLILSHFGPI 361

Query: 402 DEWTHTGAAFSGMLYGFLTSPVMEVNNAAVSSSSRRGQEKGIKFV----KKYADPCKSFA 461
           D+WT+ GA  +G++YGF T PV+++     S  S R  ++GI  V    +  ADPCKSF 
Sbjct: 362 DDWTNLGALIAGIVYGFFTCPVLQLG----SGGSER--QEGIVTVGPEKQNSADPCKSFL 421

Query: 462 FFAFFIMGFTSLLFFIQPPPTTLPLYD 482
            F  F+    + L  I   P   P YD
Sbjct: 422 LFTIFVAVIVTSLLLIGDGPLDFPTYD 427

BLAST of Tan0015974 vs. TAIR 10
Match: AT5G38510.2 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-80
Identity = 173/387 (44.70%), Postives = 231/387 (59.69%), Query Frame = 0

Query: 102 KKLCHVWECSTN---EKQLRSLGSYFGRLQGGGNKKKMDSLKKIEVLDTGQFKAKKELEL 161
           ++LC V   S N   +++L+ L SYFG+LQ    K  + +   I+     +    +EL+ 
Sbjct: 62  RRLCLVRASSENKITKQRLKLLDSYFGKLQNDDEKPSISTGDDID--RKAELNVNEELDS 121

Query: 162 LDAYFEKVDKDLDTHNHARSNTFGEQHEGKSVFTSFPFEDCARDDEIQIRHEKPNDMMHR 221
           L AY +K+ KD  +     S     + EG SV +        R   I+  +  P      
Sbjct: 122 LSAYLDKLQKDAKSKGLVSSTLDVVKSEGGSVASKL------RKTGIE-NNNSPFQQF-- 181

Query: 222 SIPKTTQMQEDEVCDLYLISTLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLI 281
                 + Q ++  + Y +S L S+N+ V LFE A+PV+N+++ L SLP LYGAKINDLI
Sbjct: 182 ----DDEDQAEDTLNFYAVSILASINVGVCLFEAAAPVRNNNMGLLSLPLLYGAKINDLI 241

Query: 282 LVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSF 341
           L GEWWRLVTPMFLHSG+ HVALS W LLTFG +VCRDYG FTF LIY+LGGVSGN  SF
Sbjct: 242 LAGEWWRLVTPMFLHSGIPHVALSSWALLTFGPKVCRDYGLFTFCLIYILGGVSGNFMSF 301

Query: 342 LHTPEPTVGGTGPMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPI 401
           LHT +PTVGGTGP FALIGAWL  Q QNK+++  +  +++F KA+I      ILS+ GPI
Sbjct: 302 LHTADPTVGGTGPAFALIGAWLVDQNQNKEMIKSNEYEDLFQKAIIMTGFGLILSHFGPI 361

Query: 402 DEWTHTGAAFSGMLYGFLTSPVMEVNNAAVSSSSRRGQEKGIKFV----KKYADPCKSFA 461
           D+WT+ GA  +G++YGF T PV+++     S  S R  ++GI  V    +  ADPCKSF 
Sbjct: 362 DDWTNLGALIAGIVYGFFTCPVLQLG----SGGSER--QEGIVTVGPEKQNSADPCKSFL 421

Query: 462 FFAFFIMGFTSLLFFIQPPPTTLPLYD 482
            F  F+    + L  I   P   P YD
Sbjct: 422 LFTIFVAVIVTSLLLIGDGPLDFPTYD 427

BLAST of Tan0015974 vs. TAIR 10
Match: AT1G25290.2 (RHOMBOID-like protein 10 )

HSP 1 Score: 86.7 bits (213), Expect = 5.7e-17
Identity = 51/182 (28.02%), Postives = 90/182 (49.45%), Query Frame = 0

Query: 238 STLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSGVL 297
           + L+++N+ +++ ++AS  K            +GAKIN LI  G+ WRL T   LH+  +
Sbjct: 131 NVLLAINVIMYIAQIASDGK---------VLTWGAKINSLIERGQLWRLATASVLHANPM 190

Query: 298 HVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHTPEPTVGGTGPMFALIG 357
           H+ ++C++L + G       GP  F  +Y+   V+ +  S+     P+VG +G +F L+G
Sbjct: 191 HLMINCYSLNSIGPTAESLGGPKRFLAVYLTSAVASSAMSYWFNKAPSVGASGAIFGLVG 250

Query: 358 AWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLT 417
           +   +  ++K +V     D M    +IA  ++  L +   ID W H G    G    +L 
Sbjct: 251 SVAVFVIRHKQMVRGGNEDLMQIAQIIALNMAMGLMS-RRIDNWGHIGGLLGGTAMTWLL 302

Query: 418 SP 420
            P
Sbjct: 311 GP 302

BLAST of Tan0015974 vs. TAIR 10
Match: AT1G25290.1 (RHOMBOID-like protein 10 )

HSP 1 Score: 82.0 bits (201), Expect = 1.4e-15
Identity = 52/189 (27.51%), Postives = 91/189 (48.15%), Query Frame = 0

Query: 238 STLVSVNIAVFLFEVASPVKNSDLPLFSLPSLYGAKINDLILVGEWWRLVTPMFLHSGVL 297
           + L+++N+ +++ ++AS  K            +GAKIN LI  G+ WRL T   LH+  +
Sbjct: 131 NVLLAINVIMYIAQIASDGK---------VLTWGAKINSLIERGQLWRLATASVLHANPM 190

Query: 298 HVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVS-------GNLTSFLHTPEPTVGGTG 357
           H+ ++C++L + G       GP  F  +Y+   V+       G+  S+     P+VG +G
Sbjct: 191 HLMINCYSLNSIGPTAESLGGPKRFLAVYLTSAVAKPILRVLGSAMSYWFNKAPSVGASG 250

Query: 358 PMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDEWTHTGAAFSG 417
            +F L+G+   +  ++K +V     D M    +IA  ++  L +   ID W H G    G
Sbjct: 251 AIFGLVGSVAVFVIRHKQMVRGGNEDLMQIAQIIALNMAMGLMS-RRIDNWGHIGGLLGG 309

Query: 418 MLYGFLTSP 420
               +L  P
Sbjct: 311 TAMTWLLGP 309

BLAST of Tan0015974 vs. TAIR 10
Match: AT1G12750.1 (RHOMBOID-like protein 6 )

HSP 1 Score: 75.5 bits (184), Expect = 1.3e-13
Identity = 48/181 (26.52%), Postives = 92/181 (50.83%), Query Frame = 0

Query: 282 EWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCRDYGPFTFFLIYVLGGVSGNLTSFLHT 341
           E WRL+T M+LH+G++H+ ++ + ++ FG ++ + +G     LIY++ G  G++ S L  
Sbjct: 96  EKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRIGLIYLISGFGGSILSALFL 155

Query: 342 PEP-TVGGTGPMFALIGAWLSYQFQNKDVVAKDVSDNMFFKAVIAAVISSILSNIGPIDE 401
            +  +VG +G +  L+GA LS    N  +    +   + F  +IA  I+  +  +  +D 
Sbjct: 156 QKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLSFLFIIA--INLAIGLLPWVDN 215

Query: 402 WTHTGAAFSGMLYGFLTSPVMEVNNAAVSSSSRRGQEKGIKFVKKYADPCKSFAFFAFFI 461
           + H G    G+L GF    ++ +   +     R   + G +   KY +PC+   FF   +
Sbjct: 216 FAHIG----GLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSKY-NPCQYVLFFVAAV 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFX02.3e-7944.70RHOMBOID-like protein 9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL9 P... [more]
P544936.4e-2128.74Rhomboid protease GluP OS=Bacillus subtilis (strain 168) OX=224308 GN=gluP PE=1 ... [more]
F4ICF42.0e-1427.51RHOMBOID-like protein 10, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL10... [more]
Q8VZ481.9e-1226.52RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 P... [more]
F4JBM47.1e-1228.10RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038883181.11.0e-19375.05RHOMBOID-like protein 9, chloroplastic isoform X2 [Benincasa hispida][more]
XP_038883174.11.9e-19275.10RHOMBOID-like protein 9, chloroplastic isoform X1 [Benincasa hispida][more]
XP_004137292.32.8e-19173.22RHOMBOID-like protein 9, chloroplastic isoform X2 [Cucumis sativus][more]
XP_011653344.26.9e-19073.07RHOMBOID-like protein 9, chloroplastic isoform X1 [Cucumis sativus][more]
XP_011653345.29.0e-19073.22RHOMBOID-like protein 9, chloroplastic isoform X4 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A0A0L0I79.7e-19073.47Rhomboid domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G094530 PE... [more]
A0A1S3C0U53.1e-18873.38RHOMBOID-like protein 9, chloroplastic isoform X5 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A1S3C2H97.7e-18773.22RHOMBOID-like protein 9, chloroplastic isoform X4 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A1S4E1D51.5e-18571.81RHOMBOID-like protein 9, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A1S4E0Q33.6e-18471.66RHOMBOID-like protein 9, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G38510.11.6e-8044.70Rhomboid-related intramembrane serine protease family protein [more]
AT5G38510.21.6e-8044.70Rhomboid-related intramembrane serine protease family protein [more]
AT1G25290.25.7e-1728.02RHOMBOID-like protein 10 [more]
AT1G25290.11.4e-1527.51RHOMBOID-like protein 10 [more]
AT1G12750.11.3e-1326.52RHOMBOID-like protein 6 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 233..424
e-value: 8.7E-33
score: 115.6
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 236..421
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 281..419
e-value: 1.7E-29
score: 102.8
NoneNo IPR availablePANTHERPTHR43731:SF18RHOMBOID-LIKE PROTEIN 9, CHLOROPLASTICcoord: 97..475
NoneNo IPR availablePANTHERPTHR43731RHOMBOID PROTEASEcoord: 97..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0015974.1Tan0015974.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity