Tan0015617 (gene) Snake gourd v1

BLAST of Tan0015617 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 545.4 bits (1404), Expect = 7.3e-154
Identity = 300/569 (52.72%), Postives = 380/569 (66.78%), Query Frame = 0

Query: 9   LLTLFTLLIL-------FSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHCR 68
           L+ LFTL  L        S+   A+      + IC+    P +C+S LPN    ++S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 69  FTLHHALAHARAFLALIDVQL------TLFPSLSALHDCRSLAAANLDFISNTFQIVNTT 128
            +L  +L+ AR F+++ID +L          ++ AL DC+ LA+  +D++ ++ Q  + +
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTAD-S 124

Query: 129 TNTLPFYQADEMVSLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSL 188
           T TL   +A+++ + LSA ITNE+TC EGL  +    GL    S  +  D KLY + L+L
Sbjct: 125 TKTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGL----SGDLFNDTKLYGVSLAL 184

Query: 189 FKLGWV-----SEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLL 248
           F  GWV     S  +  P+    K FG  NG L LKM+ + RA Y  +  RK      LL
Sbjct: 185 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRK------LL 244

Query: 249 QTNTSEDDGILVNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESV 308
           Q   S+ D + V+ IV V QNGT +FTTINAAIAAAPN T  +NGYFLI+VTAG+Y E V
Sbjct: 245 Q---SDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYV 304

Query: 309 LVPKNKRYVLMIGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGAN 368
            VPKNKRYV+MIG+G NQT+IT N++VVDG TTFNS T I+ G  F GVN+TI NTAG  
Sbjct: 305 EVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPT 364

Query: 369 KHQAVALRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNC 428
           K QAVALR   D + FY+C FE YQDTLYTHSLRQFYRECD+YGT+DFIFGNAAVVLQNC
Sbjct: 365 KGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNC 424

Query: 429 NLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNT-KTYLGRPWKEY 488
           NLY R P  GQ N +TAQGRTDPNQNTGT+IH CTI+P  +LA S  T KTYLGRPWKEY
Sbjct: 425 NLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEY 484

Query: 489 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQW-AVGVINAT 548
           SRTV MQ++ID F++P+GW  W G   L+T YYAE+NN+GPGSDT+ RV W    VINAT
Sbjct: 485 SRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINAT 544

Query: 549 VANNFTVSTFLGADQWLPRTTVPYTAGLI 558
            A+NFTV+ FL  + W+ +T VP+  GLI
Sbjct: 545 DASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

BLAST of Tan0015617 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-146
Identity = 296/590 (50.17%), Postives = 380/590 (64.41%), Query Frame = 0

Query: 5   SPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHCRFTL 64
           SPI +  L TL     S  ++      ++ IC++T  P +CKS  P+S   V  +  F++
Sbjct: 3   SPIFI--LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSI 62

Query: 65  HHALAHARAFLALIDVQLTLFPSLS------ALHDCRSLAAANLDFISNTFQIVNTTT-- 124
             +L+ +R F+  +D  +     LS      AL DCR LA   +D++  +F+ VN T+  
Sbjct: 63  RKSLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAK 122

Query: 125 ---NTLPFYQADEMVSLLSAVITNEETCYEGL-----------VGSGLGLGLVDKVSNSI 184
                L F +AD++ +LLSA +TNE+TC EGL           V +G+ L LV+      
Sbjct: 123 TSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVN------ 182

Query: 185 SFDRKLYSLYLSLFKLGWVSEGVK----------APELPNMKHFGL-GNGALKLKMSPKD 244
             D KL  + L+LF  GWV +  K          +    + K F L  NGAL LKM+ K 
Sbjct: 183 --DTKLLGVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKT 242

Query: 245 RAYYERLVHRKKPAGRRLLQTNTSEDDG--ILVNGIVGVDQNGTYDFTTINAAIAAAPNN 304
           +A YE L  RK   G     +N   DDG  +L++ IV V Q+GT +FT I AA+AAAPNN
Sbjct: 243 KAVYESLSRRKLADG----DSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNN 302

Query: 305 TAAANGYFLIFVTAGIYNESVLVPKNKRYVLMIGEGNNQTIITANKNVVDGSTTFNSGTV 364
           T  + G+FLI+VTAGIY E + + KNKRY++MIG+G NQT++T N++VVDG TTFNS T 
Sbjct: 303 TDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATF 362

Query: 365 IVEGTGFFGVNLTITNTAGANKHQAVALRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRE 424
            V    F  VN+T  NTAG  KHQAVALR  AD + FY+C FE YQDTLYTHSLRQFYRE
Sbjct: 363 AVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRE 422

Query: 425 CDIYGTIDFIFGNAAVVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPT 484
           CD+YGT+DFIFGNAAVV QNCNLY R P+  QFNA+TAQGR+DPNQNTGTSI NCTIKP 
Sbjct: 423 CDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPA 482

Query: 485 AELAASPNT-KTYLGRPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNS 544
            +L +S  T KTYLGRPWKEYSRTVYMQS+ID F++P GW+EW+G   L+T YYAE+NN+
Sbjct: 483 DDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNT 542

Query: 545 GPGSDTSRRVQW-AVGVINATVANNFTVSTFLGADQWLPRTTVPYTAGLI 558
           GPGS+T+ RV W    VIN+T A NFTV+       W+ +T VPYT+GLI
Sbjct: 543 GPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Tan0015617 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 520.0 bits (1338), Expect = 3.3e-146
Identity = 294/588 (50.00%), Postives = 383/588 (65.14%), Query Frame = 0

Query: 4   SSPISLLTLF-TLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLP-NSLATVHSHCR 63
           S  + L+TLF +L  LF +  + +P    ++ IC++T  P FCKS  P  S   V  + R
Sbjct: 3   SLKLFLVTLFLSLQTLFIASQTLLPSN-SSSTICKTTPDPKFCKSVFPQTSQGDVREYGR 62

Query: 64  FTLHHALAHARAFLALIDVQLTLFPSL------SALHDCRSLAAANLDFISNTFQIVNTT 123
           F+L  +L  +R F   ID  L    +L       AL DCR LA+   D++  +F+ VN T
Sbjct: 63  FSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNIT 122

Query: 124 TN--TLPFYQADEMVSLLSAVITNEETCYEGL---------VGSGLGLGLVDKVSNSISF 183
           T+  TL F +ADE+ +LLSA +TNE+TC +G+         + +G+ L L++        
Sbjct: 123 TSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLIN-------- 182

Query: 184 DRKLYSLYLSLFKLGWVSEGVKA-------------PELPNMKHFGLGNGALKLKMSPKD 243
           D KL+S+ L+LF  GWV +  K                    +HF   NGAL LKM+   
Sbjct: 183 DTKLFSVSLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHF--RNGALPLKMTEHT 242

Query: 244 RAYYERLVHRKKPAGRRLLQTNTSEDDGILVNGIVGVDQNGTYDFTTINAAIAAAPNNTA 303
           RA YE L        RR L  + ++ + +LV+ IV V+QNGT +FTTI  A+ +APN T 
Sbjct: 243 RAVYESL-------SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTD 302

Query: 304 AANGYFLIFVTAGIYNESVLVPKNKRYVLMIGEGNNQTIITANKNVVDGSTTFNSGTVIV 363
              GYF+I+VT+G+Y E+V++ KNKRY++MIG+G N+T++T N+NVVDG TTFNS T  V
Sbjct: 303 GTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAV 362

Query: 364 EGTGFFGVNLTITNTAGANKHQAVALRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRECD 423
               F  VN+T  NTAG  KHQAVA+R SAD + FY+C FE YQDTLYTHSLRQFYRECD
Sbjct: 363 TSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECD 422

Query: 424 IYGTIDFIFGNAAVVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAE 483
           IYGT+DFIFGNAAVV Q+CNLY R P+  QFNA+TAQGRTDPNQNTG SIHNCTIKP  +
Sbjct: 423 IYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADD 482

Query: 484 LAASPNT-KTYLGRPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGP 543
           L +S  T KTYLGRPWKEYSRTV+MQS+ID  ++P GW+EW+G   L+T YYAE+NN+G 
Sbjct: 483 LVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGS 542

Query: 544 GSDTSRRVQW-AVGVINATVANNFTVSTFLGADQWLPRTTVPYTAGLI 558
           GS T+ RV W    VIN+T ANNFTV  FL  D W+ ++ VPY +GL+
Sbjct: 543 GSSTTDRVVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Tan0015617 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 9.7e-114
Identity = 235/553 (42.50%), Postives = 326/553 (58.95%), Query Frame = 0

Query: 21  SFSSAVPGPVRTAEICQSTTSPPFCKSFL---PNSLATVHSHCRFTLHHALAHARAFLAL 80
           S S + P P+     C+ST  P  C++ L    +S +  + + +FT+   L  AR    +
Sbjct: 73  SQSPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKV 132

Query: 81  I---------DVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVNTTTNTLPFYQADEMV 140
           I         D   +    +SA+ DC  LA  +++++    + +      +     D + 
Sbjct: 133 INRFAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELK-AAELMTAALVDRVT 192

Query: 141 SLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLFKLGWVSEGVKAP 200
           SLL  V+TN++TC +GLV +  G      +   +    +LYS+ L L     ++  +K  
Sbjct: 193 SLLGGVVTNQQTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHA-LNRNLKRY 252

Query: 201 ELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRK--KPAGRRLLQTNTSEDDGILVNGIV 260
           +    K FG GN  ++  +    +   +     K  + A R L +   +    ILV   V
Sbjct: 253 KGSKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAV 312

Query: 261 GVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNKRYVLMIGEGN 320
            V    T +F TI  A+AAAPN+T    GYF+I+  AG+Y E V++   KR +++IG+G 
Sbjct: 313 TVGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGI 372

Query: 321 NQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVALRVSADTTTF 380
           N+TII+ N + +DG TT+NS T  V G  F  V++T  NTAG  KHQAVA+R +AD +TF
Sbjct: 373 NKTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTF 432

Query: 381 YTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARLPLSGQFNALT 440
           Y C FEGYQDTLY HSLRQFYRECDIYGTIDFIFGNAA + QNCN+YAR P++ Q NA+T
Sbjct: 433 YRCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVT 492

Query: 441 AQGRTDPNQNTGTSIHNCTIKPTAELAASP-NTKTYLGRPWKEYSRTVYMQSFIDAFIDP 500
           A GRTDPNQ TG SI NCTI    +LAA P +T T+LGRPWK YSRTVY+QS+I   + P
Sbjct: 493 AHGRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQP 552

Query: 501 AGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWA-VGVINATVANNFTVSTFLGADQW 558
            GW EW+GT  L+T  Y E++N GPG+DTS+RVQW+   ++N   A NFTV  F   D W
Sbjct: 553 VGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTW 612

BLAST of Tan0015617 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.5e-111
Identity = 228/538 (42.38%), Postives = 322/538 (59.85%), Query Frame = 0

Query: 36  CQSTTSPPFCKSFL---PNSLATVHSHCRFTLHHALAHARAFLALIDVQLTLFPS----- 95
           C+ST  P  C++ L    +S +  + + +FT+   L  A     +I        S     
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 96  ----LSALHDCRSLAAANLDFISNTFQIVNTTTNTLPFYQADEMVSLLSAVITNEETCYE 155
               + A+ DC  L+  +++++  T      T   +     + + SLLS V+TN++TC +
Sbjct: 143 TAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 156 GLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLFKLGWVSEGVKAPELPNMKHFGLGNGAL 215
           GLV +  G      + + +    +LYS+ L L     ++  +K  +    K  G GN   
Sbjct: 203 GLVEAKSGFAAA--IGSPMGNLTRLYSISLGLVSHA-LNRNLKRFKASKGKILGGGNSTY 262

Query: 216 KLKMSPKDRAYYERLVHRK--KPAGRRLLQTNTSEDDGILVNGIVGVDQNGTYDFTTINA 275
           +  +    +   +   + K  +   R L +   +    ILV+  V V    + +FTTI  
Sbjct: 263 REPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFTTITD 322

Query: 276 AIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNKRYVLMIGEGNNQTIITANKNVVDGS 335
           AIAAAPNNT   +GYF+I+   G+Y E ++VP NK+ ++++G+G N+TIIT N NVVDG 
Sbjct: 323 AIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNVVDGW 382

Query: 336 TTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVALRVSADTTTFYTCIFEGYQDTLYTH 395
           TT+N  +  V G  F  V++T  NTAG  KHQAVALR +A+ ++FY C FEGYQDTLY H
Sbjct: 383 TTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLYVH 442

Query: 396 SLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSI 455
           SLRQFYRECDIYGT+DFIFGNAA + QNCN+YAR P++ Q NA+TA GR DPNQNTG SI
Sbjct: 443 SLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGISI 502

Query: 456 HNCTIKPTAELAASP-NTKTYLGRPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTS 515
            NCTIK   +LAA P +  T+LGRPWK YSRTV+MQS+I   + P GW EW+GT+ L+T 
Sbjct: 503 INCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGLDTI 562

Query: 516 YYAEFNNSGPGSDTSRRVQW-AVGVINATVANNFTVSTFLGADQWLPRTTVPYTAGLI 558
           YY E++N GPG++T++RVQW    ++N   A NFTV  F   D WLP+T +P+  GL+
Sbjct: 563 YYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIPFYGGLL 616

BLAST of Tan0015617 vs. NCBI nr
Match: XP_023007148.1 (probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita maxima])

HSP 1 Score: 912.1 bits (2356), Expect = 2.3e-261
Identity = 456/561 (81.28%), Postives = 489/561 (87.17%), Query Frame = 0

Query: 1   MASSSPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHC 60
           M SSS I LLT  TLL LFS  SSA+  PV  A+IC STTSP FCK+FLPNSLATVHSHC
Sbjct: 1   MGSSSAIPLLTFLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLHHALAHARAFLALIDVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVN----TTT 120
           RFTL HALAHAR FLAL+D  LTLFPSL+ALHDCRSLAAANLDF+SNTF  VN    TTT
Sbjct: 61  RFTLRHALAHARTFLALVDTHLTLFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTT 120

Query: 121 NTLPFYQADEMVSLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLF 180
           N LPFY+A+EMVSLLSAVITNE TCYEGL+ SG   GLVDKV  +ISFD KLYSLYLSLF
Sbjct: 121 NALPFYKAEEMVSLLSAVITNENTCYEGLMSSGSASGLVDKVLKAISFDTKLYSLYLSLF 180

Query: 181 KLGWVSEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLLQTNTSE 240
           K+GWVS  VKAP +P MKHFG G G LKL MSPK+RAYYERL+HR KP  RRLLQTN++E
Sbjct: 181 KMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRSKPPARRLLQTNSNE 240

Query: 241 DDGILVNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNK 300
           DDGILVNGIV VDQNGTYDFTTINAAI AAPNN+ AA+GYFLI VTAG+YNESV+VPK+K
Sbjct: 241 DDGILVNGIVAVDQNGTYDFTTINAAIQAAPNNSVAADGYFLIIVTAGVYNESVMVPKHK 300

Query: 301 RYVLMIGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVA 360
           +YVLMIGEGNNQTIIT NKNVVDGSTTFNSGT IVEGTGFFGVNLTITNTAG NK QAVA
Sbjct: 301 KYVLMIGEGNNQTIITGNKNVVDGSTTFNSGTFIVEGTGFFGVNLTITNTAGPNKFQAVA 360

Query: 361 LRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARL 420
           LRVSAD TTFY+CIFEGYQDTLY HSLRQFYRECDIYGTIDFI G+AAVV QNCN+YARL
Sbjct: 361 LRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARL 420

Query: 421 PLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNTKTYLGRPWKEYSRTVYMQ 480
           P+ GQ+  +TAQGRTD NQNTG SIHNCTIKPTA+LAASP  K+Y GRPWKEY+RTVYMQ
Sbjct: 421 PMKGQYIVITAQGRTDRNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYMQ 480

Query: 481 SFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWAVGVINATVANNFTVS 540
           SFIDAFIDP GW  WDGTLNLNTSYYAEFNNSGPGSDTSRR +WAVG+INATVANNFTVS
Sbjct: 481 SFIDAFIDPEGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNFTVS 540

Query: 541 TFLGADQWLPRTTVPYTAGLI 558
            FLG DQWLP TTVPYT GLI
Sbjct: 541 PFLGGDQWLPPTTVPYTGGLI 561

BLAST of Tan0015617 vs. NCBI nr
Match: XP_023534721.1 (probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 911.8 bits (2355), Expect = 3.0e-261
Identity = 457/560 (81.61%), Postives = 491/560 (87.68%), Query Frame = 0

Query: 1   MASSSPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHC 60
           M SSS ISLLT  TLL LFS  SSA+  PV  A+IC STTSP FCK+FLPNSLATVHSHC
Sbjct: 1   MGSSSAISLLTFLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLHHALAHARAFLALIDVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVN---TTTN 120
           RFTL HALAHAR+FLAL+D  L LFPSL+ALHDCRSLAAANLDF+SNTF  VN   TTTN
Sbjct: 61  RFTLRHALAHARSFLALVDTHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTN 120

Query: 121 TLPFYQADEMVSLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLFK 180
            LPFY+ADEMVSLLSAVITNE TCYEGL+ S  G G VDKV  +ISFD KLYSLYLSLFK
Sbjct: 121 ALPFYKADEMVSLLSAVITNENTCYEGLLSS--GSGSVDKVLKAISFDTKLYSLYLSLFK 180

Query: 181 LGWVSEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLLQTNTSED 240
           +GWVS  VKAP +P MKHFG G G LKL MSPK+RAYYERL+HR+KP  RRLLQTN++ED
Sbjct: 181 MGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNSNED 240

Query: 241 DGILVNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNKR 300
           DGILVNGIV VD NGTYDFTTINAAI AAPNN+ AA+GYFLIFVTAG+YNESV+VPK+K+
Sbjct: 241 DGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPKHKK 300

Query: 301 YVLMIGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVAL 360
           YVLMIGEGNNQTIIT  KNV DGSTTFNSGTVIVEGTGFFGVNLTITNTAG NK QAVAL
Sbjct: 301 YVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQAVAL 360

Query: 361 RVSADTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARLP 420
           RVSAD TTFY+CIFEGYQDTLY HSLRQFYRECDIYGTIDFI G+AAVV QNCN+YARLP
Sbjct: 361 RVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARLP 420

Query: 421 LSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNTKTYLGRPWKEYSRTVYMQS 480
           + GQ+  +TAQGRTDPNQNTG SIHNCTIKPTA+LAASP  K+Y GRPWKEY+RTVYMQS
Sbjct: 421 MKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYMQS 480

Query: 481 FIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWAVGVINATVANNFTVST 540
           FIDAFIDPAGW  WDGTLNLNTSYYAEFNNSGPGSDTSRR +WAVG+INATVANNFTVS 
Sbjct: 481 FIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNFTVSP 540

Query: 541 FLGADQWLPRTTVPYTAGLI 558
           FLG DQWLP TTVPYT GLI
Sbjct: 541 FLGGDQWLPPTTVPYTGGLI 558

BLAST of Tan0015617 vs. NCBI nr
Match: KAG6605296.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 909.1 bits (2348), Expect = 1.9e-260
Identity = 456/564 (80.85%), Postives = 493/564 (87.41%), Query Frame = 0

Query: 1   MASSSPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHC 60
           M SSS ISLL + TLL LFS  SSA+  PV  A+IC STTSP FCK+FLPNSLATVHSHC
Sbjct: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLHHALAHARAFLALIDVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVN-----TT 120
           RFTL HALAHAR+FLAL++  L LFPSL+ALHDCRSLAAANLDF+SNTF  VN     TT
Sbjct: 61  RFTLRHALAHARSFLALVNTHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTT 120

Query: 121 TNTLPFYQADEMVSLLSAVITNEETCYEGLV--GSGLGLGLVDKVSNSISFDRKLYSLYL 180
           TN LPFY+ADEMVSLLSAVITNE TCYEGL+  GSG G G VDKV  +ISFD KLYSLYL
Sbjct: 121 TNALPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSVDKVLKAISFDTKLYSLYL 180

Query: 181 SLFKLGWVSEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLLQTN 240
           SLFK+GWVS  VKAP +P MKHFG G G LKL MSPK+RAYYERL+HR+KP  RRLLQTN
Sbjct: 181 SLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTN 240

Query: 241 TSEDDGILVNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVP 300
           ++EDDGILVNGIV VD NGTYDFTTINAAI AAPNN+ AA+GYFLIFVTAG+YNESV+VP
Sbjct: 241 SNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVP 300

Query: 301 KNKRYVLMIGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQ 360
           K+K+YVLMIGEGNNQTIIT  KNV DGSTTFNSGTVIVEGTGFFGVNLTITNTAG NK Q
Sbjct: 301 KHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQ 360

Query: 361 AVALRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLY 420
           AVALRVSAD TTFY+CIFEGYQDTLY HSLRQFYRECDIYGTIDFI G+AAVV QNCN+Y
Sbjct: 361 AVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIY 420

Query: 421 ARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNTKTYLGRPWKEYSRTV 480
           ARLP+ GQ+  +TAQGRTDPNQNTG SIHNCTIKPTA+LAA+P  K+Y GRPWKEY+RTV
Sbjct: 421 ARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAATPTIKSYFGRPWKEYARTV 480

Query: 481 YMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWAVGVINATVANNF 540
           YMQSFIDAFIDPAGW  WDGTLNLNTSYYAEFNNSGPGSDTSRR +WAVG+INATVANNF
Sbjct: 481 YMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNF 540

Query: 541 TVSTFLGADQWLPRTTVPYTAGLI 558
           TVS FLG DQWLP TTVPYT GLI
Sbjct: 541 TVSPFLGGDQWLPPTTVPYTGGLI 564

BLAST of Tan0015617 vs. NCBI nr
Match: XP_022947145.1 (probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita moschata])

HSP 1 Score: 907.9 bits (2345), Expect = 4.3e-260
Identity = 457/563 (81.17%), Postives = 492/563 (87.39%), Query Frame = 0

Query: 1   MASSSPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHC 60
           M SSS ISLL + TLL LFS  SSA+  PV  A+IC STTSP FCK+FLPNSLATVHSHC
Sbjct: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLHHALAHARAFLALIDVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVN--TTTNT 120
           RFTL HALAHAR+FLAL+D  L LFPSL+ALHDCRSLAAANLDF+SNTF  VN  TTTN 
Sbjct: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNA 120

Query: 121 LPFYQADEMVSLLSAVITNEETCYEGLV----GSGLGLGLVDKVSNSISFDRKLYSLYLS 180
           LPFY+ADEMVSLLSAVITNE TCYEGL+    GSG G G VDKV  +ISFD KLYSLYLS
Sbjct: 121 LPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLS 180

Query: 181 LFKLGWVSEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLLQTNT 240
           LFK+GWVS  VKAP +P MKHFG G G LKL MSPK+RAYYERL+HR+KP  RRLLQTN+
Sbjct: 181 LFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNS 240

Query: 241 SEDDGILVNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPK 300
           +EDDGILVNGIV VD NGTYDFTTINAAI AAPNN+ AA+GYFLIFVTAG+YNESV+VPK
Sbjct: 241 NEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPK 300

Query: 301 NKRYVLMIGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQA 360
           +K+YVLMIGEGNNQTIIT  KNV DGSTTFNSGTVIVEGTGFFGVNLTITNTAG NK QA
Sbjct: 301 HKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQA 360

Query: 361 VALRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYA 420
           VALRVSAD TTFY+CIFEGYQDTLY HSLRQFYRECDIYGTIDFI G+AAVV QNCN+YA
Sbjct: 361 VALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYA 420

Query: 421 RLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNTKTYLGRPWKEYSRTVY 480
           RLP+ GQ+  +TAQGRTDPNQNTG SIHNCTIKPTA+LAASP  K+Y GRPWKEY+RTVY
Sbjct: 421 RLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVY 480

Query: 481 MQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWAVGVINATVANNFT 540
           MQSFIDAFIDPAGW  WDGTLNLNTSYYAEFNNSG GSDTSRR +WAVG+INATVANNFT
Sbjct: 481 MQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFT 540

Query: 541 VSTFLGADQWLPRTTVPYTAGLI 558
           VS FLG DQWLP TTVPYT GLI
Sbjct: 541 VSPFLGGDQWLPPTTVPYTGGLI 563

BLAST of Tan0015617 vs. NCBI nr
Match: XP_022928019.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 902.9 bits (2332), Expect = 1.4e-258
Identity = 454/556 (81.65%), Postives = 492/556 (88.49%), Query Frame = 0

Query: 2   ASSSPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHCR 61
           +SS+PISLL  F+LL LF+S SSAVP    T E+CQS TSP FCKSFLP+S A+VHSHCR
Sbjct: 3   SSSNPISLLLFFSLLFLFTS-SSAVP----TVEVCQSATSPSFCKSFLPSSPASVHSHCR 62

Query: 62  FTLHHALAHARAFLALIDVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVNTTTNTLPF 121
           +TLHHAL  AR F+ L+D+QLTLFPSLSALHDCRSLA ANLDFIS+T   VNTTTN LPF
Sbjct: 63  YTLHHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTNALPF 122

Query: 122 YQADEMVSLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLFKLGWV 181
           YQADEM+SLLSAVITN +TC++GL GSGLGLG VDKV  +ISFDR+LYSLYLSLFK+GWV
Sbjct: 123 YQADEMISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFKMGWV 182

Query: 182 SEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLLQTNTSEDDGIL 241
           S+ +KAP+LP   HFG G G LKLKMSPKDRAYYERLVH KKPA RRLLQTN + DDGIL
Sbjct: 183 SKNMKAPKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVHGKKPAARRLLQTNYA-DDGIL 242

Query: 242 VNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNKRYVLM 301
           VNG+V VDQNG YDFTTIN AIAAAPN + AANGYFLIFVTAGIY ESV+VPK+K+YVLM
Sbjct: 243 VNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLM 302

Query: 302 IGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVALRVSA 361
           IGEGNN+TIIT NKNVVDGSTTFNS TVIVEGTGFFG NLTITNTAGA KHQAVALRVSA
Sbjct: 303 IGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSA 362

Query: 362 DTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARLPLSGQ 421
           D TTFY+CIFEGYQDTLY HSLRQFYRECDIYGT+DFIFGNAAVVLQNCN+YARLPLSGQ
Sbjct: 363 DMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQ 422

Query: 422 FNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNTKTYLGRPWKEYSRTVYMQSFIDA 481
           FNALTAQGRTD NQNTG  IH CTIKPT +LAASP TK+YLGRPWKE+SRTVYMQSFIDA
Sbjct: 423 FNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDA 482

Query: 482 FIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWAVGVINATVANNFTVSTFLGA 541
           FIDPAGWKEWDGTLNLNTSYYAEF+NSGPGSDTS+R QWAVGVINATVAN FTVS  L  
Sbjct: 483 FIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSEILAG 542

Query: 542 DQWLPRTTVPYTAGLI 558
           DQWLP T VPY+ GLI
Sbjct: 543 DQWLPPTAVPYSGGLI 552

BLAST of Tan0015617 vs. ExPASy TrEMBL
Match: A0A6J1L6X0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499728 PE=3 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 1.1e-261
Identity = 456/561 (81.28%), Postives = 489/561 (87.17%), Query Frame = 0

Query: 1   MASSSPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHC 60
           M SSS I LLT  TLL LFS  SSA+  PV  A+IC STTSP FCK+FLPNSLATVHSHC
Sbjct: 1   MGSSSAIPLLTFLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLHHALAHARAFLALIDVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVN----TTT 120
           RFTL HALAHAR FLAL+D  LTLFPSL+ALHDCRSLAAANLDF+SNTF  VN    TTT
Sbjct: 61  RFTLRHALAHARTFLALVDTHLTLFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTT 120

Query: 121 NTLPFYQADEMVSLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLF 180
           N LPFY+A+EMVSLLSAVITNE TCYEGL+ SG   GLVDKV  +ISFD KLYSLYLSLF
Sbjct: 121 NALPFYKAEEMVSLLSAVITNENTCYEGLMSSGSASGLVDKVLKAISFDTKLYSLYLSLF 180

Query: 181 KLGWVSEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLLQTNTSE 240
           K+GWVS  VKAP +P MKHFG G G LKL MSPK+RAYYERL+HR KP  RRLLQTN++E
Sbjct: 181 KMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRSKPPARRLLQTNSNE 240

Query: 241 DDGILVNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNK 300
           DDGILVNGIV VDQNGTYDFTTINAAI AAPNN+ AA+GYFLI VTAG+YNESV+VPK+K
Sbjct: 241 DDGILVNGIVAVDQNGTYDFTTINAAIQAAPNNSVAADGYFLIIVTAGVYNESVMVPKHK 300

Query: 301 RYVLMIGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVA 360
           +YVLMIGEGNNQTIIT NKNVVDGSTTFNSGT IVEGTGFFGVNLTITNTAG NK QAVA
Sbjct: 301 KYVLMIGEGNNQTIITGNKNVVDGSTTFNSGTFIVEGTGFFGVNLTITNTAGPNKFQAVA 360

Query: 361 LRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARL 420
           LRVSAD TTFY+CIFEGYQDTLY HSLRQFYRECDIYGTIDFI G+AAVV QNCN+YARL
Sbjct: 361 LRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARL 420

Query: 421 PLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNTKTYLGRPWKEYSRTVYMQ 480
           P+ GQ+  +TAQGRTD NQNTG SIHNCTIKPTA+LAASP  K+Y GRPWKEY+RTVYMQ
Sbjct: 421 PMKGQYIVITAQGRTDRNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYMQ 480

Query: 481 SFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWAVGVINATVANNFTVS 540
           SFIDAFIDP GW  WDGTLNLNTSYYAEFNNSGPGSDTSRR +WAVG+INATVANNFTVS
Sbjct: 481 SFIDAFIDPEGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNFTVS 540

Query: 541 TFLGADQWLPRTTVPYTAGLI 558
            FLG DQWLP TTVPYT GLI
Sbjct: 541 PFLGGDQWLPPTTVPYTGGLI 561

BLAST of Tan0015617 vs. ExPASy TrEMBL
Match: A0A6J1G612 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451102 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 2.1e-260
Identity = 457/563 (81.17%), Postives = 492/563 (87.39%), Query Frame = 0

Query: 1   MASSSPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHC 60
           M SSS ISLL + TLL LFS  SSA+  PV  A+IC STTSP FCK+FLPNSLATVHSHC
Sbjct: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLHHALAHARAFLALIDVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVN--TTTNT 120
           RFTL HALAHAR+FLAL+D  L LFPSL+ALHDCRSLAAANLDF+SNTF  VN  TTTN 
Sbjct: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNA 120

Query: 121 LPFYQADEMVSLLSAVITNEETCYEGLV----GSGLGLGLVDKVSNSISFDRKLYSLYLS 180
           LPFY+ADEMVSLLSAVITNE TCYEGL+    GSG G G VDKV  +ISFD KLYSLYLS
Sbjct: 121 LPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLS 180

Query: 181 LFKLGWVSEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLLQTNT 240
           LFK+GWVS  VKAP +P MKHFG G G LKL MSPK+RAYYERL+HR+KP  RRLLQTN+
Sbjct: 181 LFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNS 240

Query: 241 SEDDGILVNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPK 300
           +EDDGILVNGIV VD NGTYDFTTINAAI AAPNN+ AA+GYFLIFVTAG+YNESV+VPK
Sbjct: 241 NEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPK 300

Query: 301 NKRYVLMIGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQA 360
           +K+YVLMIGEGNNQTIIT  KNV DGSTTFNSGTVIVEGTGFFGVNLTITNTAG NK QA
Sbjct: 301 HKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQA 360

Query: 361 VALRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYA 420
           VALRVSAD TTFY+CIFEGYQDTLY HSLRQFYRECDIYGTIDFI G+AAVV QNCN+YA
Sbjct: 361 VALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYA 420

Query: 421 RLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNTKTYLGRPWKEYSRTVY 480
           RLP+ GQ+  +TAQGRTDPNQNTG SIHNCTIKPTA+LAASP  K+Y GRPWKEY+RTVY
Sbjct: 421 RLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVY 480

Query: 481 MQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWAVGVINATVANNFT 540
           MQSFIDAFIDPAGW  WDGTLNLNTSYYAEFNNSG GSDTSRR +WAVG+INATVANNFT
Sbjct: 481 MQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFT 540

Query: 541 VSTFLGADQWLPRTTVPYTAGLI 558
           VS FLG DQWLP TTVPYT GLI
Sbjct: 541 VSPFLGGDQWLPPTTVPYTGGLI 563

BLAST of Tan0015617 vs. ExPASy TrEMBL
Match: A0A6J1EJM8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 6.7e-259
Identity = 454/556 (81.65%), Postives = 492/556 (88.49%), Query Frame = 0

Query: 2   ASSSPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHCR 61
           +SS+PISLL  F+LL LF+S SSAVP    T E+CQS TSP FCKSFLP+S A+VHSHCR
Sbjct: 3   SSSNPISLLLFFSLLFLFTS-SSAVP----TVEVCQSATSPSFCKSFLPSSPASVHSHCR 62

Query: 62  FTLHHALAHARAFLALIDVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVNTTTNTLPF 121
           +TLHHAL  AR F+ L+D+QLTLFPSLSALHDCRSLA ANLDFIS+T   VNTTTN LPF
Sbjct: 63  YTLHHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTNALPF 122

Query: 122 YQADEMVSLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLFKLGWV 181
           YQADEM+SLLSAVITN +TC++GL GSGLGLG VDKV  +ISFDR+LYSLYLSLFK+GWV
Sbjct: 123 YQADEMISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFKMGWV 182

Query: 182 SEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLLQTNTSEDDGIL 241
           S+ +KAP+LP   HFG G G LKLKMSPKDRAYYERLVH KKPA RRLLQTN + DDGIL
Sbjct: 183 SKNMKAPKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVHGKKPAARRLLQTNYA-DDGIL 242

Query: 242 VNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNKRYVLM 301
           VNG+V VDQNG YDFTTIN AIAAAPN + AANGYFLIFVTAGIY ESV+VPK+K+YVLM
Sbjct: 243 VNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLM 302

Query: 302 IGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVALRVSA 361
           IGEGNN+TIIT NKNVVDGSTTFNS TVIVEGTGFFG NLTITNTAGA KHQAVALRVSA
Sbjct: 303 IGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSA 362

Query: 362 DTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARLPLSGQ 421
           D TTFY+CIFEGYQDTLY HSLRQFYRECDIYGT+DFIFGNAAVVLQNCN+YARLPLSGQ
Sbjct: 363 DMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQ 422

Query: 422 FNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNTKTYLGRPWKEYSRTVYMQSFIDA 481
           FNALTAQGRTD NQNTG  IH CTIKPT +LAASP TK+YLGRPWKE+SRTVYMQSFIDA
Sbjct: 423 FNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDA 482

Query: 482 FIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWAVGVINATVANNFTVSTFLGA 541
           FIDPAGWKEWDGTLNLNTSYYAEF+NSGPGSDTS+R QWAVGVINATVAN FTVS  L  
Sbjct: 483 FIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSEILAG 542

Query: 542 DQWLPRTTVPYTAGLI 558
           DQWLP T VPY+ GLI
Sbjct: 543 DQWLPPTAVPYSGGLI 552

BLAST of Tan0015617 vs. ExPASy TrEMBL
Match: A0A6J1I6B8 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 3.1e-256
Identity = 452/557 (81.15%), Postives = 490/557 (87.97%), Query Frame = 0

Query: 2   ASSSPISLLTLFTLLILF-SSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHC 61
           +SS+PISLLT F+LL LF  + SSAVP    T E+CQS TSP FCKSFLP+S A+VHSHC
Sbjct: 3   SSSNPISLLTFFSLLFLFLFTSSSAVP----TVEVCQSATSPSFCKSFLPSSPASVHSHC 62

Query: 62  RFTLHHALAHARAFLALIDVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVNTTTNTLP 121
           R+TLHHAL  AR F+ L+D+QLTL PS SALHDCRSLA ANLDFIS+T   VNTTTN LP
Sbjct: 63  RYTLHHALVQARTFITLVDLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVNTTTNALP 122

Query: 122 FYQADEMVSLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLFKLGW 181
           FYQADEM+SLLSAVITN +TC++GL GS  GLGLVDKV  +ISFDR+LYSLYLSLFK+GW
Sbjct: 123 FYQADEMISLLSAVITNVDTCHDGLAGS--GLGLVDKVVKAISFDRQLYSLYLSLFKMGW 182

Query: 182 VSEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLLQTNTSEDDGI 241
           VS+ +KAP+LP   HFG GNG LKLKMSPKDRAYYERL+H KKPA RRLLQTN + DDGI
Sbjct: 183 VSKTMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLLQTNNA-DDGI 242

Query: 242 LVNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNKRYVL 301
           LVNG+V VDQNG YDFTTIN AIAAAPN + AANGYFLIFVTAGIY ESV+VPK+K+YVL
Sbjct: 243 LVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVL 302

Query: 302 MIGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVALRVS 361
           MIGEGNN+TIIT NKNVVDGSTTFNS TVIVEGTGFFG NLTITNTAGA KHQAVALRVS
Sbjct: 303 MIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVS 362

Query: 362 ADTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARLPLSG 421
           AD TTFY+CIFEGYQDTLY HSLRQFYRECDIYGT+DFIFGNAAVVLQNCN+YARLPLSG
Sbjct: 363 ADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSG 422

Query: 422 QFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNTKTYLGRPWKEYSRTVYMQSFID 481
           QFNALTAQGRTD NQNTG  IH CTIKPT +LAASP TK+YLGRPWKE+SRTVYMQSFID
Sbjct: 423 QFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFID 482

Query: 482 AFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWAVGVINATVANNFTVSTFLG 541
           AFIDPAGWKEWDGTLNLNTSYYAEF+NSGPGSDTSRR QWAVGVINATVAN FTVS  L 
Sbjct: 483 AFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILA 542

Query: 542 ADQWLPRTTVPYTAGLI 558
            DQWLP T VPY+ GLI
Sbjct: 543 GDQWLPPTAVPYSGGLI 552

BLAST of Tan0015617 vs. ExPASy TrEMBL
Match: A0A5A7TRW1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002210 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 4.1e-256
Identity = 449/559 (80.32%), Postives = 486/559 (86.94%), Query Frame = 0

Query: 1   MASSSPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHC 60
           M SS+PIS+LT  TLL   S  SSAVP    TAEICQSTTSPPFC  FLPNS A+VHSHC
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSAVP----TAEICQSTTSPPFCNFFLPNSPASVHSHC 60

Query: 61  RFTLHHALAHARAFLALIDVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVNTTTNT-L 120
           RFTLHHAL HAR FL+L++  L LFPSLS LHDCRSLA ANLDF+S TF IVN+TT T L
Sbjct: 61  RFTLHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLL 120

Query: 121 PFYQADEMVSLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLFKLG 180
           P+Y A E ++L+SAVITNE+TCYEGL G    +GLVDKV  +ISFD+KLYSLYLSLFK+G
Sbjct: 121 PYYDAHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMG 180

Query: 181 WVSEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAG-RRLLQTNTSEDD 240
           WVS+ +KAP LP MKHFG+G G LKLKMSPKDRAYYERLVHR KP G RRLLQTN  +DD
Sbjct: 181 WVSKDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTN-YQDD 240

Query: 241 GILVNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNKRY 300
           GI++NGIVGVDQNGTYDFT I AAIAAAPN T  A GYFLIFV AGIYNE+VL+PK KRY
Sbjct: 241 GIVINGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRY 300

Query: 301 VLMIGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVALR 360
           VL+IGEGNNQTIIT NKNVVDGSTTFNS TV VEGTGF GVNLTI+NTAGA KHQAVALR
Sbjct: 301 VLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALR 360

Query: 361 VSADTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARLPL 420
           VSAD  T Y CIFEGYQDTLYTHSLRQFYRECDIYGT+DFIFGNAAVVLQNCN+YARLPL
Sbjct: 361 VSADNATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPL 420

Query: 421 SGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNTKTYLGRPWKEYSRTVYMQSF 480
           SGQFNA+TAQGRTDPNQNTGTSIHNCTIK T ELAASP TK+YLGRPWK+YSRTVYMQSF
Sbjct: 421 SGQFNAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSF 480

Query: 481 IDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWAVGVINATVANNFTVSTF 540
           ID+FIDPAGWKEWDGT+NLNTSYYAEFNNSGPGSDTS+RV WAVGVINATVAN+FTVS F
Sbjct: 481 IDSFIDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQF 540

Query: 541 LGADQWLPRTTVPYTAGLI 558
           L  D+WLP T VPYT GLI
Sbjct: 541 LAGDRWLPPTDVPYTGGLI 554

BLAST of Tan0015617 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 545.4 bits (1404), Expect = 5.2e-155
Identity = 300/569 (52.72%), Postives = 380/569 (66.78%), Query Frame = 0

Query: 9   LLTLFTLLIL-------FSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHCR 68
           L+ LFTL  L        S+   A+      + IC+    P +C+S LPN    ++S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 69  FTLHHALAHARAFLALIDVQL------TLFPSLSALHDCRSLAAANLDFISNTFQIVNTT 128
            +L  +L+ AR F+++ID +L          ++ AL DC+ LA+  +D++ ++ Q  + +
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTAD-S 124

Query: 129 TNTLPFYQADEMVSLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSL 188
           T TL   +A+++ + LSA ITNE+TC EGL  +    GL    S  +  D KLY + L+L
Sbjct: 125 TKTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGL----SGDLFNDTKLYGVSLAL 184

Query: 189 FKLGWV-----SEGVKAPELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRKKPAGRRLL 248
           F  GWV     S  +  P+    K FG  NG L LKM+ + RA Y  +  RK      LL
Sbjct: 185 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRK------LL 244

Query: 249 QTNTSEDDGILVNGIVGVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESV 308
           Q   S+ D + V+ IV V QNGT +FTTINAAIAAAPN T  +NGYFLI+VTAG+Y E V
Sbjct: 245 Q---SDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYV 304

Query: 309 LVPKNKRYVLMIGEGNNQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGAN 368
            VPKNKRYV+MIG+G NQT+IT N++VVDG TTFNS T I+ G  F GVN+TI NTAG  
Sbjct: 305 EVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPT 364

Query: 369 KHQAVALRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNC 428
           K QAVALR   D + FY+C FE YQDTLYTHSLRQFYRECD+YGT+DFIFGNAAVVLQNC
Sbjct: 365 KGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNC 424

Query: 429 NLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAELAASPNT-KTYLGRPWKEY 488
           NLY R P  GQ N +TAQGRTDPNQNTGT+IH CTI+P  +LA S  T KTYLGRPWKEY
Sbjct: 425 NLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEY 484

Query: 489 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQW-AVGVINAT 548
           SRTV MQ++ID F++P+GW  W G   L+T YYAE+NN+GPGSDT+ RV W    VINAT
Sbjct: 485 SRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINAT 544

Query: 549 VANNFTVSTFLGADQWLPRTTVPYTAGLI 558
            A+NFTV+ FL  + W+ +T VP+  GLI
Sbjct: 545 DASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

BLAST of Tan0015617 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 521.5 bits (1342), Expect = 8.1e-148
Identity = 296/590 (50.17%), Postives = 380/590 (64.41%), Query Frame = 0

Query: 5   SPISLLTLFTLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLPNSLATVHSHCRFTL 64
           SPI +  L TL     S  ++      ++ IC++T  P +CKS  P+S   V  +  F++
Sbjct: 3   SPIFI--LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSI 62

Query: 65  HHALAHARAFLALIDVQLTLFPSLS------ALHDCRSLAAANLDFISNTFQIVNTTT-- 124
             +L+ +R F+  +D  +     LS      AL DCR LA   +D++  +F+ VN T+  
Sbjct: 63  RKSLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAK 122

Query: 125 ---NTLPFYQADEMVSLLSAVITNEETCYEGL-----------VGSGLGLGLVDKVSNSI 184
                L F +AD++ +LLSA +TNE+TC EGL           V +G+ L LV+      
Sbjct: 123 TSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVN------ 182

Query: 185 SFDRKLYSLYLSLFKLGWVSEGVK----------APELPNMKHFGL-GNGALKLKMSPKD 244
             D KL  + L+LF  GWV +  K          +    + K F L  NGAL LKM+ K 
Sbjct: 183 --DTKLLGVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKT 242

Query: 245 RAYYERLVHRKKPAGRRLLQTNTSEDDG--ILVNGIVGVDQNGTYDFTTINAAIAAAPNN 304
           +A YE L  RK   G     +N   DDG  +L++ IV V Q+GT +FT I AA+AAAPNN
Sbjct: 243 KAVYESLSRRKLADG----DSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNN 302

Query: 305 TAAANGYFLIFVTAGIYNESVLVPKNKRYVLMIGEGNNQTIITANKNVVDGSTTFNSGTV 364
           T  + G+FLI+VTAGIY E + + KNKRY++MIG+G NQT++T N++VVDG TTFNS T 
Sbjct: 303 TDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATF 362

Query: 365 IVEGTGFFGVNLTITNTAGANKHQAVALRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRE 424
            V    F  VN+T  NTAG  KHQAVALR  AD + FY+C FE YQDTLYTHSLRQFYRE
Sbjct: 363 AVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRE 422

Query: 425 CDIYGTIDFIFGNAAVVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPT 484
           CD+YGT+DFIFGNAAVV QNCNLY R P+  QFNA+TAQGR+DPNQNTGTSI NCTIKP 
Sbjct: 423 CDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPA 482

Query: 485 AELAASPNT-KTYLGRPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNS 544
            +L +S  T KTYLGRPWKEYSRTVYMQS+ID F++P GW+EW+G   L+T YYAE+NN+
Sbjct: 483 DDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNT 542

Query: 545 GPGSDTSRRVQW-AVGVINATVANNFTVSTFLGADQWLPRTTVPYTAGLI 558
           GPGS+T+ RV W    VIN+T A NFTV+       W+ +T VPYT+GLI
Sbjct: 543 GPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Tan0015617 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 520.0 bits (1338), Expect = 2.3e-147
Identity = 294/588 (50.00%), Postives = 383/588 (65.14%), Query Frame = 0

Query: 4   SSPISLLTLF-TLLILFSSFSSAVPGPVRTAEICQSTTSPPFCKSFLP-NSLATVHSHCR 63
           S  + L+TLF +L  LF +  + +P    ++ IC++T  P FCKS  P  S   V  + R
Sbjct: 3   SLKLFLVTLFLSLQTLFIASQTLLPSN-SSSTICKTTPDPKFCKSVFPQTSQGDVREYGR 62

Query: 64  FTLHHALAHARAFLALIDVQLTLFPSL------SALHDCRSLAAANLDFISNTFQIVNTT 123
           F+L  +L  +R F   ID  L    +L       AL DCR LA+   D++  +F+ VN T
Sbjct: 63  FSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNIT 122

Query: 124 TN--TLPFYQADEMVSLLSAVITNEETCYEGL---------VGSGLGLGLVDKVSNSISF 183
           T+  TL F +ADE+ +LLSA +TNE+TC +G+         + +G+ L L++        
Sbjct: 123 TSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLIN-------- 182

Query: 184 DRKLYSLYLSLFKLGWVSEGVKA-------------PELPNMKHFGLGNGALKLKMSPKD 243
           D KL+S+ L+LF  GWV +  K                    +HF   NGAL LKM+   
Sbjct: 183 DTKLFSVSLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHF--RNGALPLKMTEHT 242

Query: 244 RAYYERLVHRKKPAGRRLLQTNTSEDDGILVNGIVGVDQNGTYDFTTINAAIAAAPNNTA 303
           RA YE L        RR L  + ++ + +LV+ IV V+QNGT +FTTI  A+ +APN T 
Sbjct: 243 RAVYESL-------SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTD 302

Query: 304 AANGYFLIFVTAGIYNESVLVPKNKRYVLMIGEGNNQTIITANKNVVDGSTTFNSGTVIV 363
              GYF+I+VT+G+Y E+V++ KNKRY++MIG+G N+T++T N+NVVDG TTFNS T  V
Sbjct: 303 GTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAV 362

Query: 364 EGTGFFGVNLTITNTAGANKHQAVALRVSADTTTFYTCIFEGYQDTLYTHSLRQFYRECD 423
               F  VN+T  NTAG  KHQAVA+R SAD + FY+C FE YQDTLYTHSLRQFYRECD
Sbjct: 363 TSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECD 422

Query: 424 IYGTIDFIFGNAAVVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTAE 483
           IYGT+DFIFGNAAVV Q+CNLY R P+  QFNA+TAQGRTDPNQNTG SIHNCTIKP  +
Sbjct: 423 IYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADD 482

Query: 484 LAASPNT-KTYLGRPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGP 543
           L +S  T KTYLGRPWKEYSRTV+MQS+ID  ++P GW+EW+G   L+T YYAE+NN+G 
Sbjct: 483 LVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGS 542

Query: 544 GSDTSRRVQW-AVGVINATVANNFTVSTFLGADQWLPRTTVPYTAGLI 558
           GS T+ RV W    VIN+T ANNFTV  FL  D W+ ++ VPY +GL+
Sbjct: 543 GSSTTDRVVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Tan0015617 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 412.1 bits (1058), Expect = 6.9e-115
Identity = 235/553 (42.50%), Postives = 326/553 (58.95%), Query Frame = 0

Query: 21  SFSSAVPGPVRTAEICQSTTSPPFCKSFL---PNSLATVHSHCRFTLHHALAHARAFLAL 80
           S S + P P+     C+ST  P  C++ L    +S +  + + +FT+   L  AR    +
Sbjct: 73  SQSPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKV 132

Query: 81  I---------DVQLTLFPSLSALHDCRSLAAANLDFISNTFQIVNTTTNTLPFYQADEMV 140
           I         D   +    +SA+ DC  LA  +++++    + +      +     D + 
Sbjct: 133 INRFAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELK-AAELMTAALVDRVT 192

Query: 141 SLLSAVITNEETCYEGLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLFKLGWVSEGVKAP 200
           SLL  V+TN++TC +GLV +  G      +   +    +LYS+ L L     ++  +K  
Sbjct: 193 SLLGGVVTNQQTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHA-LNRNLKRY 252

Query: 201 ELPNMKHFGLGNGALKLKMSPKDRAYYERLVHRK--KPAGRRLLQTNTSEDDGILVNGIV 260
           +    K FG GN  ++  +    +   +     K  + A R L +   +    ILV   V
Sbjct: 253 KGSKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAV 312

Query: 261 GVDQNGTYDFTTINAAIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNKRYVLMIGEGN 320
            V    T +F TI  A+AAAPN+T    GYF+I+  AG+Y E V++   KR +++IG+G 
Sbjct: 313 TVGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGI 372

Query: 321 NQTIITANKNVVDGSTTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVALRVSADTTTF 380
           N+TII+ N + +DG TT+NS T  V G  F  V++T  NTAG  KHQAVA+R +AD +TF
Sbjct: 373 NKTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTF 432

Query: 381 YTCIFEGYQDTLYTHSLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARLPLSGQFNALT 440
           Y C FEGYQDTLY HSLRQFYRECDIYGTIDFIFGNAA + QNCN+YAR P++ Q NA+T
Sbjct: 433 YRCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVT 492

Query: 441 AQGRTDPNQNTGTSIHNCTIKPTAELAASP-NTKTYLGRPWKEYSRTVYMQSFIDAFIDP 500
           A GRTDPNQ TG SI NCTI    +LAA P +T T+LGRPWK YSRTVY+QS+I   + P
Sbjct: 493 AHGRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQP 552

Query: 501 AGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRVQWA-VGVINATVANNFTVSTFLGADQW 558
            GW EW+GT  L+T  Y E++N GPG+DTS+RVQW+   ++N   A NFTV  F   D W
Sbjct: 553 VGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTW 612

BLAST of Tan0015617 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 404.8 bits (1039), Expect = 1.1e-112
Identity = 228/538 (42.38%), Postives = 322/538 (59.85%), Query Frame = 0

Query: 36  CQSTTSPPFCKSFL---PNSLATVHSHCRFTLHHALAHARAFLALIDVQLTLFPS----- 95
           C+ST  P  C++ L    +S +  + + +FT+   L  A     +I        S     
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 96  ----LSALHDCRSLAAANLDFISNTFQIVNTTTNTLPFYQADEMVSLLSAVITNEETCYE 155
               + A+ DC  L+  +++++  T      T   +     + + SLLS V+TN++TC +
Sbjct: 143 TAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 156 GLVGSGLGLGLVDKVSNSISFDRKLYSLYLSLFKLGWVSEGVKAPELPNMKHFGLGNGAL 215
           GLV +  G      + + +    +LYS+ L L     ++  +K  +    K  G GN   
Sbjct: 203 GLVEAKSGFAAA--IGSPMGNLTRLYSISLGLVSHA-LNRNLKRFKASKGKILGGGNSTY 262

Query: 216 KLKMSPKDRAYYERLVHRK--KPAGRRLLQTNTSEDDGILVNGIVGVDQNGTYDFTTINA 275
           +  +    +   +   + K  +   R L +   +    ILV+  V V    + +FTTI  
Sbjct: 263 REPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFTTITD 322

Query: 276 AIAAAPNNTAAANGYFLIFVTAGIYNESVLVPKNKRYVLMIGEGNNQTIITANKNVVDGS 335
           AIAAAPNNT   +GYF+I+   G+Y E ++VP NK+ ++++G+G N+TIIT N NVVDG 
Sbjct: 323 AIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNVVDGW 382

Query: 336 TTFNSGTVIVEGTGFFGVNLTITNTAGANKHQAVALRVSADTTTFYTCIFEGYQDTLYTH 395
           TT+N  +  V G  F  V++T  NTAG  KHQAVALR +A+ ++FY C FEGYQDTLY H
Sbjct: 383 TTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLYVH 442

Query: 396 SLRQFYRECDIYGTIDFIFGNAAVVLQNCNLYARLPLSGQFNALTAQGRTDPNQNTGTSI 455
           SLRQFYRECDIYGT+DFIFGNAA + QNCN+YAR P++ Q NA+TA GR DPNQNTG SI
Sbjct: 443 SLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGISI 502

Query: 456 HNCTIKPTAELAASP-NTKTYLGRPWKEYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTS 515
            NCTIK   +LAA P +  T+LGRPWK YSRTV+MQS+I   + P GW EW+GT+ L+T 
Sbjct: 503 INCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGLDTI 562

Query: 516 YYAEFNNSGPGSDTSRRVQW-AVGVINATVANNFTVSTFLGADQWLPRTTVPYTAGLI 558
           YY E++N GPG++T++RVQW    ++N   A NFTV  F   D WLP+T +P+  GL+
Sbjct: 563 YYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIPFYGGLL 616