Tan0015614 (gene) Snake gourd v1

Overview
NameTan0015614
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionserine/threonine-protein kinase BLUS1-like
LocationLG06: 76856225 .. 76858059 (-)
RNA-Seq ExpressionTan0015614
SyntenyTan0015614
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAATATTAGTGTTTTTTTTTTCAGCAAAAATGATTATTTGCTTTACTATACAAGTTTCCCTCCATTTAATCAGTTAAATTTGTTAACCAAGTTTGTAAAACCACTTTAACTACCTAATAGGAAGAGTCGTTATTGAAAAGAGTGAAAAAAAAAAAATTGCGACAAGAATCATACGAGAATCCAATCCTGTAAAAAAAAGAACCTTGTTTCAGTCAAAAAAATCATAGTCAGATTTTGTTATTTATTTCCGTAAATCATGTAAATTGTTGATGATATTTTCCTTTATTTTCTGCTCTGTCACAAACTGCAATTTTACCCCCTCATCTATTTTTTTTTCACAATGGAGCACCCCCAATTTCAAGATCAAGAAACCACCGCCGCCGGAAGAATCCGTTTCCAGTTTCCGACCACCGCCGACGGCTACAAAATCCTCAGTCAAATCGGCGTCAGCGATCACAGCACCGTCTACATGGCGATCTGCAAGTCTGCAGACTCGACGATCGTCGCTGTAAAAATTATCAATATCGACATCGACATCGACCCGATTTCAATCATGGACATAAAATCTCTATCACTCATCAATCACCCTAATATCCTCAGCCCTCATTGTTGTTTCACCGCCGACGACGGCCGCCTCTGGATCGTCCTGCCGTTCATCCCCTCCGGCCCTTTACAAGCCATAGTCTCCAACAGGTTTCCAAGTGGTTTGCCGGATCAATTCATGGCCATTCTTTTAAACCAACTCCTTCATGGCTTGGTTTGTCTTCATGAAATGGGCTACGTTCATAAAGCCATTAAAGCAAGCAACATCTTTTTCGATTCGCAAGGCTCCGTTCAGCTCTCGGATTTGAGCCTTTCGAACCATACGGGCGATGACAATGATCATGATGATGATAGGTTTTCGGTGCCGTATTGGGCACCGCCGGAGGACGAGTATAGCTTCAAATCGGATGTGTGGTCAATAGGGATTTTAGCAATGGAAATTGCCTATGGGGGGCCTCCCATTTCGAGCTCAAGAATTGTCAAGTCTCAAATGAAGAACATCTCGAAACGGTTTCCATATGATTTGAATCTCGATAAGAAATCAGGGAAGAGCGTCATCGATAGAAAATTTGGAGATTTGTTCAAGGATTTTGTGAGTTCTTGCCTCATTAAGGATCGGTTTGCAAGGCCTTCAGCTTCGAGTTTGTTGGCTCATCCATTCATCAAAAAAGGAGAAAGTTCAAATTTTTTGTTCAAGAGTATTGCAAAAGGAGTGGTTGGAATTGGAGATAGATTCGAACAAGATAAAGATAGAATCATGGGGTTGATGAAAAACGAAATGAAACTAGAAAATTATAGTCAAAATGAGAAGATTACAAAGATTAGTGGATGGGATTATAATAACTTTTTATTTGAACTCGAGCCCGATGAGGAATCATTAGGGAAAAGGGTTAGATTTTGTGGAGAAACAATAATTCCATTTAGAGAAGAGGAGATCAAATTTGATATGCCATTATTTCTTCAAAGAATGGATAGTTTAAGTGGTCGAACAAATGGGAGTCAAAATGAGGATAAGAAAAATAAGGTTCAAGTTATGGATAGTGAAAGTTTAGCAAAATTATTATCTTCATTGTTAGAAAATATGGATCATCAAAGGGAGATGATTATGATGTTGATAACTCAATTTGGTTATTTGAGTGAAAGGGATTTGGAAATGACAAAAGAAATAAAAAAGTTAGAGGCAGAATTGGCCTCTGAGAAAGAAAGAAGCTTTCAATTAGAATTGGATTATGAAGTTTTAAAGCTTAAACTTCCTGATTCTGAGGAGGAAGAAGATGATTCTGATAATTGA

mRNA sequence

GAAATATTAGTGTTTTTTTTTTCAGCAAAAATGATTATTTGCTTTACTATACAAGTTTCCCTCCATTTAATCAGTTAAATTTGTTAACCAAGTTTGTAAAACCACTTTAACTACCTAATAGGAAGAGTCGTTATTGAAAAGAGTGAAAAAAAAAAAATTGCGACAAGAATCATACGAGAATCCAATCCTGTAAAAAAAAGAACCTTGTTTCAGTCAAAAAAATCATAGTCAGATTTTGTTATTTATTTCCGTAAATCATGTAAATTGTTGATGATATTTTCCTTTATTTTCTGCTCTGTCACAAACTGCAATTTTACCCCCTCATCTATTTTTTTTTCACAATGGAGCACCCCCAATTTCAAGATCAAGAAACCACCGCCGCCGGAAGAATCCGTTTCCAGTTTCCGACCACCGCCGACGGCTACAAAATCCTCAGTCAAATCGGCGTCAGCGATCACAGCACCGTCTACATGGCGATCTGCAAGTCTGCAGACTCGACGATCGTCGCTGTAAAAATTATCAATATCGACATCGACATCGACCCGATTTCAATCATGGACATAAAATCTCTATCACTCATCAATCACCCTAATATCCTCAGCCCTCATTGTTGTTTCACCGCCGACGACGGCCGCCTCTGGATCGTCCTGCCGTTCATCCCCTCCGGCCCTTTACAAGCCATAGTCTCCAACAGGTTTCCAAGTGGTTTGCCGGATCAATTCATGGCCATTCTTTTAAACCAACTCCTTCATGGCTTGGTTTGTCTTCATGAAATGGGCTACGTTCATAAAGCCATTAAAGCAAGCAACATCTTTTTCGATTCGCAAGGCTCCGTTCAGCTCTCGGATTTGAGCCTTTCGAACCATACGGGCGATGACAATGATCATGATGATGATAGGTTTTCGGTGCCGTATTGGGCACCGCCGGAGGACGAGTATAGCTTCAAATCGGATGTGTGGTCAATAGGGATTTTAGCAATGGAAATTGCCTATGGGGGGCCTCCCATTTCGAGCTCAAGAATTGTCAAGTCTCAAATGAAGAACATCTCGAAACGGTTTCCATATGATTTGAATCTCGATAAGAAATCAGGGAAGAGCGTCATCGATAGAAAATTTGGAGATTTGTTCAAGGATTTTGTGAGTTCTTGCCTCATTAAGGATCGGTTTGCAAGGCCTTCAGCTTCGAGTTTGTTGGCTCATCCATTCATCAAAAAAGGAGAAAGTTCAAATTTTTTGTTCAAGAGTATTGCAAAAGGAGTGGTTGGAATTGGAGATAGATTCGAACAAGATAAAGATAGAATCATGGGGTTGATGAAAAACGAAATGAAACTAGAAAATTATAGTCAAAATGAGAAGATTACAAAGATTAGTGGATGGGATTATAATAACTTTTTATTTGAACTCGAGCCCGATGAGGAATCATTAGGGAAAAGGGTTAGATTTTGTGGAGAAACAATAATTCCATTTAGAGAAGAGGAGATCAAATTTGATATGCCATTATTTCTTCAAAGAATGGATAGTTTAAGTGGTCGAACAAATGGGAGTCAAAATGAGGATAAGAAAAATAAGGTTCAAGTTATGGATAGTGAAAGTTTAGCAAAATTATTATCTTCATTGTTAGAAAATATGGATCATCAAAGGGAGATGATTATGATGTTGATAACTCAATTTGGTTATTTGAGTGAAAGGGATTTGGAAATGACAAAAGAAATAAAAAAGTTAGAGGCAGAATTGGCCTCTGAGAAAGAAAGAAGCTTTCAATTAGAATTGGATTATGAAGTTTTAAAGCTTAAACTTCCTGATTCTGAGGAGGAAGAAGATGATTCTGATAATTGA

Coding sequence (CDS)

ATGGAGCACCCCCAATTTCAAGATCAAGAAACCACCGCCGCCGGAAGAATCCGTTTCCAGTTTCCGACCACCGCCGACGGCTACAAAATCCTCAGTCAAATCGGCGTCAGCGATCACAGCACCGTCTACATGGCGATCTGCAAGTCTGCAGACTCGACGATCGTCGCTGTAAAAATTATCAATATCGACATCGACATCGACCCGATTTCAATCATGGACATAAAATCTCTATCACTCATCAATCACCCTAATATCCTCAGCCCTCATTGTTGTTTCACCGCCGACGACGGCCGCCTCTGGATCGTCCTGCCGTTCATCCCCTCCGGCCCTTTACAAGCCATAGTCTCCAACAGGTTTCCAAGTGGTTTGCCGGATCAATTCATGGCCATTCTTTTAAACCAACTCCTTCATGGCTTGGTTTGTCTTCATGAAATGGGCTACGTTCATAAAGCCATTAAAGCAAGCAACATCTTTTTCGATTCGCAAGGCTCCGTTCAGCTCTCGGATTTGAGCCTTTCGAACCATACGGGCGATGACAATGATCATGATGATGATAGGTTTTCGGTGCCGTATTGGGCACCGCCGGAGGACGAGTATAGCTTCAAATCGGATGTGTGGTCAATAGGGATTTTAGCAATGGAAATTGCCTATGGGGGGCCTCCCATTTCGAGCTCAAGAATTGTCAAGTCTCAAATGAAGAACATCTCGAAACGGTTTCCATATGATTTGAATCTCGATAAGAAATCAGGGAAGAGCGTCATCGATAGAAAATTTGGAGATTTGTTCAAGGATTTTGTGAGTTCTTGCCTCATTAAGGATCGGTTTGCAAGGCCTTCAGCTTCGAGTTTGTTGGCTCATCCATTCATCAAAAAAGGAGAAAGTTCAAATTTTTTGTTCAAGAGTATTGCAAAAGGAGTGGTTGGAATTGGAGATAGATTCGAACAAGATAAAGATAGAATCATGGGGTTGATGAAAAACGAAATGAAACTAGAAAATTATAGTCAAAATGAGAAGATTACAAAGATTAGTGGATGGGATTATAATAACTTTTTATTTGAACTCGAGCCCGATGAGGAATCATTAGGGAAAAGGGTTAGATTTTGTGGAGAAACAATAATTCCATTTAGAGAAGAGGAGATCAAATTTGATATGCCATTATTTCTTCAAAGAATGGATAGTTTAAGTGGTCGAACAAATGGGAGTCAAAATGAGGATAAGAAAAATAAGGTTCAAGTTATGGATAGTGAAAGTTTAGCAAAATTATTATCTTCATTGTTAGAAAATATGGATCATCAAAGGGAGATGATTATGATGTTGATAACTCAATTTGGTTATTTGAGTGAAAGGGATTTGGAAATGACAAAAGAAATAAAAAAGTTAGAGGCAGAATTGGCCTCTGAGAAAGAAAGAAGCTTTCAATTAGAATTGGATTATGAAGTTTTAAAGCTTAAACTTCCTGATTCTGAGGAGGAAGAAGATGATTCTGATAATTGA

Protein sequence

MEHPQFQDQETTAAGRIRFQFPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINIDIDIDPISIMDIKSLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSGLPDQFMAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGDDNDHDDDRFSVPYWAPPEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKRFPYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFLFKSIAKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQNEKITKISGWDYNNFLFELEPDEESLGKRVRFCGETIIPFREEEIKFDMPLFLQRMDSLSGRTNGSQNEDKKNKVQVMDSESLAKLLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKERSFQLELDYEVLKLKLPDSEEEEDDSDN
Homology
BLAST of Tan0015614 vs. ExPASy Swiss-Prot
Match: O23304 (Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.4e-58
Identity = 159/502 (31.67%), Postives = 260/502 (51.79%), Query Frame = 0

Query: 16  RIRFQFPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINID---IDIDPISIM 75
           R + +FP  A+ Y+I+ +IGV   ++VY AIC   +S +VA+K I++D    D D +   
Sbjct: 3   RNKLEFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLDQSRADFDSLR-R 62

Query: 76  DIKSLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSGLPDQFMAILL 135
           + K++SL++HPNIL+ +C FT  D  LW+V+PF+  G L +IVS+ FPSGLP+  +++ L
Sbjct: 63  ETKTMSLLSHPNILNAYCSFTV-DRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFL 122

Query: 136 NQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSL----------SNHTGDDNDH 195
            + L+ +  LH+ G++H+ IKA NI  DS GSV+L+D  +          S+ T   +  
Sbjct: 123 KETLNAISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLR 182

Query: 196 DDDRFSVPYWAPPE-----DEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISK 255
             D    PYW  PE       Y FK+D+WS GI A+E+A+G PP+S    +KS +  I+K
Sbjct: 183 LTDIAGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITK 242

Query: 256 RF---PYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGES 315
           RF    Y++N    S K   ++KF   F++ V  CL +D   RPSA  LL HPF K  + 
Sbjct: 243 RFHFSDYEINTSGSSKKG--NKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKG 302

Query: 316 SNFLFKSIAKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQNEKITK---ISGWDYNNFL 375
            +F+ K++   +      F + +  I  +  ++   E   ++E+I K   ISGW++    
Sbjct: 303 LDFVVKNVLHSLSNAEQMFMESQILIKSVGDDDE--EEEEEDEEIVKNRRISGWNFREDD 362

Query: 376 FELEPDEESLGKRVRFCGETIIPFREEEIKFDMPLFLQRMDSLSGRTN----------GS 435
            +L P               + P  E +     P    +        N          G 
Sbjct: 363 LQLSP---------------VFPATESDSSESSPREEDQSKDKKEDDNVTITGYELGLGL 422

Query: 436 QNEDKKN---KVQVMDSESLAKLLSSLLENMDHQREMIMMLITQFGYLSE---RDLEMTK 478
            NE+ KN   +V   D + + + L  L ++++HQR  + ++I       E   R+ E+ +
Sbjct: 423 SNEEAKNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELLE 482

BLAST of Tan0015614 vs. ExPASy Swiss-Prot
Match: Q551H4 (Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fray2 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 6.1e-35
Identity = 100/329 (30.40%), Postives = 164/329 (49.85%), Query Frame = 0

Query: 11  TTAAGRI----RFQFPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINID--- 70
           TTAA  I    R  +P +AD Y++   IG      V  AIC       VA+KII+++   
Sbjct: 50  TTAASPITPKPRKNYPNSADQYELKETIGKGGSGLVQRAICLPFQEN-VAIKIIDLEHCK 109

Query: 71  -IDIDPISIMDIKSLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSG 130
            + ++ I   +I+++SL +HPN+++ H  F  ++  LW+++ F+ +G    I+   FP G
Sbjct: 110 NVSLEEIR-KEIQAMSLCHHPNVVAYHTSFVYNES-LWVIMDFLSAGSCSDIMRFSFPQG 169

Query: 131 LPDQFMAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGDDNDH 190
             +  +A +L + L  +   H+ G +H+ IK+ NI  DS G++QLSD  +S    D  + 
Sbjct: 170 FEEHVIATILKEALKAICYFHKTGRIHRDIKSGNILIDSNGNIQLSDFGVSATLIDTGET 229

Query: 191 DDDRF-SVPYWAPPE----DEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISK 250
             + F   P W  PE      Y +  D+WS GI A+E+A G  P +    +K  +  +  
Sbjct: 230 SRNTFVGTPCWMAPEIMEQVNYDYAVDIWSFGITALELARGKAPFAEYPPMKVLLLTLQN 289

Query: 251 RFPYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNF 310
             P  L  D +S       K+   FKD V  CL KD   RP  S LL H F K+ +  ++
Sbjct: 290 P-PPSLEGDGES-------KWSHSFKDLVEKCLQKDPSKRPLPSKLLEHRFFKQAKKPDY 349

Query: 311 LFKSIAKGVVGIGDRFEQDKDRIMGLMKN 327
           L + I   +  +G R++   +    +++N
Sbjct: 350 LVQHILAKLPPLGQRYQMLSEDSFAMLRN 367

BLAST of Tan0015614 vs. ExPASy Swiss-Prot
Match: G5EEN4 (Germinal center kinase 3 OS=Caenorhabditis elegans OX=6239 GN=gck-3 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 2.0e-30
Identity = 95/313 (30.35%), Postives = 157/313 (50.16%), Query Frame = 0

Query: 20  QFPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINID---IDIDPISIMDIKS 79
           ++PT    YK+   IGV   +TV+ A C   +   VA+K IN++     +D +S  +I++
Sbjct: 100 RWPTEPSAYKLDESIGVGATATVFTAYCLPRNEK-VAIKCINLEKCQTSVDELS-HEIQA 159

Query: 80  LSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPL--------QAIVSNRFPSGLPDQF- 139
           +S  NHPN++S +  F A +  LW+V+  +  G +        +AI   +   G+ D+  
Sbjct: 160 MSQCNHPNVVSYYTSFIAQE-ELWVVMRLLNCGSMLDILKRKVKAIGKEQAQFGVLDEVS 219

Query: 140 MAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSN---HTGDDNDHDD 199
           +A +L ++L GL   H  G +H+ IKA NI     G++Q++D  +S     +G D     
Sbjct: 220 IATVLREVLKGLEYFHLNGQIHRDIKAGNILLADDGTIQIADFGVSGWLASSGGDLSRQK 279

Query: 200 DRFS---VPYWAPPE-----DEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNIS 259
            R +    P W  PE       Y FK+D+WS+GILA+E+A G  P      +K  M  + 
Sbjct: 280 VRHTFVGTPCWMAPEVMEQVQGYDFKADIWSLGILAIELATGTAPYHKYPPMKVLMLTLQ 339

Query: 260 KRFPYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSN 310
              P    L+  + +    + +G  FK  +  CL KD   RP+AS LL + F KKG+   
Sbjct: 340 NDPP---TLETNAERKDQYKAYGKSFKTLIRDCLQKDPAKRPTASELLKYSFFKKGKDKK 399

BLAST of Tan0015614 vs. ExPASy Swiss-Prot
Match: Q5R495 (Serine/threonine-protein kinase OSR1 OS=Pongo abelii OX=9601 GN=OXSR1 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.3e-29
Identity = 103/352 (29.26%), Postives = 171/352 (48.58%), Query Frame = 0

Query: 26  DGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINID---IDIDPISIMDIKSLSLINH 85
           D Y++   IG    + V  A C +     VA+K IN++     +D + + +I+++S  +H
Sbjct: 15  DDYELQEVIGSGATAVVQAAYC-APKKEKVAIKRINLEKCQTSMDEL-LKEIQAMSQCHH 74

Query: 86  PNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSG------LPDQFMAILLNQLL 145
           PNI+S +  F   D  LW+V+  +  G +  I+ +    G      L +  +A +L ++L
Sbjct: 75  PNIVSYYTSFVVKD-ELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVL 134

Query: 146 HGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNH--TGDDNDHDDDR---FSVPY 205
            GL  LH+ G +H+ +KA NI     GSVQ++D  +S    TG D   +  R      P 
Sbjct: 135 EGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPC 194

Query: 206 WAPPE-----DEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKRFPYDLNLD 265
           W  PE       Y FK+D+WS GI A+E+A G  P      +K  M  +    P   +L+
Sbjct: 195 WMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPP---SLE 254

Query: 266 KKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFLFKSIAKGV 325
                  + +K+G  F+  +S CL KD   RP+A+ LL H F +K ++  FL + I +  
Sbjct: 255 TGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKILQRA 314

Query: 326 VGIGDRFEQDKDRIMGLMKNEMKLENYSQNEKITKISGWDYNNFLFELEPDE 359
             I +R ++ + R+ G      K E+           GW++++  F+ E +E
Sbjct: 315 PTISERAKKVR-RVPGSSGRLHKTED----------GGWEWSDDEFDEESEE 349

BLAST of Tan0015614 vs. ExPASy Swiss-Prot
Match: Q6P9R2 (Serine/threonine-protein kinase OSR1 OS=Mus musculus OX=10090 GN=Oxsr1 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.9e-29
Identity = 103/352 (29.26%), Postives = 171/352 (48.58%), Query Frame = 0

Query: 26  DGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINID---IDIDPISIMDIKSLSLINH 85
           D Y++   IG    + V  A C +     VA+K IN++     +D + + +I+++S  +H
Sbjct: 15  DDYELQEVIGSGATAVVQAAYC-APKKERVAIKRINLEKCQTSMDEL-LKEIQAMSQCHH 74

Query: 86  PNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSG------LPDQFMAILLNQLL 145
           PNI+S +  F   D  LW+V+  +  G +  I+ +    G      L +  +A +L ++L
Sbjct: 75  PNIVSYYTSFVVKD-ELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDEPTIATILREVL 134

Query: 146 HGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNH--TGDDNDHDDDR---FSVPY 205
            GL  LH+ G +H+ +KA NI     GSVQ++D  +S    TG D   +  R      P 
Sbjct: 135 EGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPC 194

Query: 206 WAPPE-----DEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKRFPYDLNLD 265
           W  PE       Y FK+D+WS GI A+E+A G  P      +K  M  +    P   +L+
Sbjct: 195 WMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPP---SLE 254

Query: 266 KKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFLFKSIAKGV 325
                  + +K+G  F+  +S CL KD   RP+A+ LL H F +K ++  FL + I +  
Sbjct: 255 TGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKILQRA 314

Query: 326 VGIGDRFEQDKDRIMGLMKNEMKLENYSQNEKITKISGWDYNNFLFELEPDE 359
             I +R ++ + R+ G      K E+           GW++++  F+ E +E
Sbjct: 315 PTISERSKKVR-RVPGSSGRLHKTED----------GGWEWSDDEFDEESEE 349

BLAST of Tan0015614 vs. NCBI nr
Match: XP_022138626.1 (serine/threonine-protein kinase BLUS1-like [Momordica charantia])

HSP 1 Score: 639.4 bits (1648), Expect = 2.5e-179
Identity = 338/505 (66.93%), Postives = 415/505 (82.18%), Query Frame = 0

Query: 5   QFQDQE-TTAAGRIRFQFPTTADGYKILSQIGVSDHSTVYMAICKSA-DSTIVAVKIINI 64
           Q QD E   AAGR+ FQFPT ADGYKIL QIGVSDHS VY AIC+SA +S  VAVK+I++
Sbjct: 6   QDQDPENAAAAGRLHFQFPTAADGYKILYQIGVSDHSVVYKAICESANNSAKVAVKVIDV 65

Query: 65  DIDIDPISIMDIKSLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSG 124
           D  +D   +M + SLSLI+H NILSPHCCFT  D RLW V+PFI +GPLQAI++NRFP+G
Sbjct: 66  DRHVDEFFVMKMNSLSLIDHANILSPHCCFTV-DRRLWTVMPFIHAGPLQAILANRFPNG 125

Query: 125 LPDQFMAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGDDNDH 184
           LPDQF+AILL+QLL  L+CLHE+GYVH+ IKASNIF DS+GSV+LSDL++  + GDD   
Sbjct: 126 LPDQFLAILLHQLLRSLICLHELGYVHRNIKASNIFVDSEGSVKLSDLNV--YVGDD--- 185

Query: 185 DDDRFSVPYWAPPEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKRFPYD 244
           DDD  SVPYW PP++EYSFKSDVW+IGI+A+E+AYG PPIS SR ++SQ+ NIS+RFPY+
Sbjct: 186 DDDDSSVPYWDPPDEEYSFKSDVWAIGIVALEVAYGCPPISDSRTIRSQLLNISRRFPYN 245

Query: 245 LNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFLFKSI 304
           LNLDK S KSVIDRKFG+LFKDFVSSCL+KD   RPSA+SLLAHPFIKKGE+SN LFKSI
Sbjct: 246 LNLDKMSSKSVIDRKFGELFKDFVSSCLVKDPSKRPSAASLLAHPFIKKGETSNVLFKSI 305

Query: 305 AKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQNEKITKISGWDYNNFLFELEPDEESLG 364
           AK + GI DRFE++KDR++ LMK + ++E+ ++   + K+SGWD+NNFLFELEPDEESLG
Sbjct: 306 AKDMAGIEDRFEKNKDRLVELMKKDRRVEDNNRG-NVVKVSGWDFNNFLFELEPDEESLG 365

Query: 365 KRVRFCGETIIPFREEEI---------KFDMPLFLQRMDSLS-GRTNGSQNEDKKNKVQV 424
           KRVRF GETIIP R++ +         KF++PL L+RMDSLS    +G      + +V+V
Sbjct: 366 KRVRFRGETIIPLRDDHVSLNEAGEASKFNVPLLLKRMDSLSLSGLSGRMGSRAEEEVRV 425

Query: 425 MDSESLAKLLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKERSF 484
           MDSE+LAK+L+SL+E++D QRE+IMM+I+QFG L ERD+EMTKEIKKL+AELA EKERSF
Sbjct: 426 MDSETLAKVLTSLVESIDQQREVIMMVISQFGRLLERDMEMTKEIKKLKAELAYEKERSF 485

Query: 485 QLELDYEVLKLKLPDSEEEEDDSDN 498
           QLELDYEVLKLKLPDS E+ED S+N
Sbjct: 486 QLELDYEVLKLKLPDS-EDEDKSEN 502

BLAST of Tan0015614 vs. NCBI nr
Match: XP_022958864.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita moschata])

HSP 1 Score: 605.9 bits (1561), Expect = 3.1e-169
Identity = 317/501 (63.27%), Postives = 390/501 (77.84%), Query Frame = 0

Query: 1   MEHPQFQDQETTAAGRIRFQFPTTADGYKILSQIGVSDHSTVYMAICKSADS-TIVAVKI 60
           M+  Q QDQET+AA     +FP  AD Y+I SQIG SDHSTVY AICKSAD+ TIVA+KI
Sbjct: 1   MDRRQIQDQETSAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKI 60

Query: 61  INIDIDIDPISIMDIK-SLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNR 120
           I+ +++ID  +I +I  +LS INHP+IL+PHCCFTA DGRLWIV+PFIP+GPLQAI+S R
Sbjct: 61  IDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQR 120

Query: 121 FPSGLPDQFMAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGD 180
           F +GLPDQFM+IL+NQLL  LV LHE G VHKA+KASNIFFD+ G V LSDL +S     
Sbjct: 121 FRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVS----- 180

Query: 181 DNDHDDDRFSVPYWAPPEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKR 240
             +   D  SVPYW PPE++YSFKSDVWS+GILAMEIAYG PPI   +++K+Q   IS+R
Sbjct: 181 -KEMKKDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRR 240

Query: 241 FPYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFL 300
           FPYDLNLD +SGKSV+ RKFGD  K FV SCL KDRFAR SA SLL HPF+KKGE+SNFL
Sbjct: 241 FPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENSNFL 300

Query: 301 FKSIAKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQ-NEKITKISGWDYNNFLFELEPD 360
           FKSIAKG+VGIG+ FEQDKDRI+ LMKNE++L   SQ   K+ +I+GWDYNNFLFELEPD
Sbjct: 301 FKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPD 360

Query: 361 EESLGKRVRFCGETIIPFREEE--IKFDMPLFLQRMDSLSGRTNGSQNEDKKNKVQVMDS 420
           EES GKRVRF GET+  ++E+E   KFDM   +           GS +E KK   Q MD+
Sbjct: 361 EESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMH---------GGSSDEKKKEVDQAMDT 420

Query: 421 ESLAKLLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKERSFQLE 480
           E+LA LLSSLL+N+D Q+EM+ ML+   G++ ++DLEM K+IK+LE ELA+EKE+SFQLE
Sbjct: 421 ETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLE 480

Query: 481 LDYEVLKLKLPDSEEEEDDSD 497
           L++E+LKLKLPDS+EE++  +
Sbjct: 481 LNFELLKLKLPDSDEEQEQEE 486

BLAST of Tan0015614 vs. NCBI nr
Match: KAG6574930.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 605.9 bits (1561), Expect = 3.1e-169
Identity = 317/501 (63.27%), Postives = 390/501 (77.84%), Query Frame = 0

Query: 1   MEHPQFQDQETTAAGRIRFQFPTTADGYKILSQIGVSDHSTVYMAICKSADS-TIVAVKI 60
           M+  Q QDQET+AA     +FP  AD Y+I SQIG SDHSTVY AICKSAD+ TIVA+KI
Sbjct: 1   MDRRQIQDQETSAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKI 60

Query: 61  INIDIDIDPISIMDIK-SLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNR 120
           I+ +++ID  +I +I  +LS INHP+IL+PHCCFTA DGRLWIV+PFIP+GPLQAI+S R
Sbjct: 61  IDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQR 120

Query: 121 FPSGLPDQFMAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGD 180
           F +GLPDQFM+IL+NQLL  LV LHE G VHKA+KASNIFFD+ G V LSDL +S     
Sbjct: 121 FRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVS----- 180

Query: 181 DNDHDDDRFSVPYWAPPEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKR 240
             +   D  SVPYW PPE++YSFKSDVWS+GILAMEIAYG PPI   +++K+Q   IS+R
Sbjct: 181 -KEMKKDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRR 240

Query: 241 FPYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFL 300
           FPYDLNLD +SGKSV+ RKFGD  K FV SCL KDRFAR SA SLL HPF+KKGE+SNFL
Sbjct: 241 FPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENSNFL 300

Query: 301 FKSIAKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQ-NEKITKISGWDYNNFLFELEPD 360
           FKSIAKG+VGIG+ FEQDKDRI+ LMKNE++L   SQ   K+ +I+GWDYNNFLFELEPD
Sbjct: 301 FKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPD 360

Query: 361 EESLGKRVRFCGETIIPFREEE--IKFDMPLFLQRMDSLSGRTNGSQNEDKKNKVQVMDS 420
           EES GKRVRF GET+  ++E+E   KFDM   +           GS +E KK   Q MD+
Sbjct: 361 EESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMH---------GGSSDEKKKEVDQAMDT 420

Query: 421 ESLAKLLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKERSFQLE 480
           E+LA LLSSLL+N+D Q+EM+ ML+   G++ ++DLEM K+IK+LE ELA+EKE+SFQLE
Sbjct: 421 ETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLE 480

Query: 481 LDYEVLKLKLPDSEEEEDDSD 497
           L++E+LKLKLPDS+EE++  +
Sbjct: 481 LNFELLKLKLPDSDEEQEQEE 486

BLAST of Tan0015614 vs. NCBI nr
Match: XP_023548299.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 600.5 bits (1547), Expect = 1.3e-167
Identity = 316/507 (62.33%), Postives = 392/507 (77.32%), Query Frame = 0

Query: 1   MEHPQFQDQETTAAGR------IRFQFPTTADGYKILSQIGVSDHSTVYMAICKSADS-T 60
           M+  Q QDQET+AA           +FP  AD Y+I SQIG SDHSTVY AICKSAD+ T
Sbjct: 1   MDRRQIQDQETSAASSASAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPT 60

Query: 61  IVAVKIINIDIDIDPISIMDIK-SLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQ 120
           IVA+KII+ +++IDP +I +I  +LS INHP+IL+PHCCFTA DGRLWIV+PFI +GPLQ
Sbjct: 61  IVAIKIIDSNVEIDPTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIHAGPLQ 120

Query: 121 AIVSNRFPSGLPDQFMAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSL 180
           AI+S RF +GLPDQFM+IL+NQLL  LV LHE G VHKA+KASNIFFD+ G V LSDL +
Sbjct: 121 AIISQRFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDV 180

Query: 181 SNHTGDDNDHDDDRFSVPYWAPPEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQM 240
           S       + + D  SVPYW PPE++YSFKSDVWS+GILAMEIAYG PPI   +++K+Q 
Sbjct: 181 S------KEMNKDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQF 240

Query: 241 KNISKRFPYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKG 300
             IS+RFPYDLNLD +SGKSV+ RKFGD  K FV SCL KDRFAR SA SLL HPF+KKG
Sbjct: 241 SKISRRFPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKG 300

Query: 301 ESSNFLFKSIAKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQ-NEKITKISGWDYNNFL 360
           E+SNFLFKSIAKG+VGIG+ FEQDKDRI+ LMKNE++L   SQ   K+ +I+GWDYNNFL
Sbjct: 301 ENSNFLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFL 360

Query: 361 FELEPDEESLGKRVRFCGETIIPFREEE--IKFDMPLFLQRMDSLSGRTNGSQNEDKKNK 420
           FELEPDEES GKRVRF GET+  ++E+E   KFDM   +          +G  +++KK  
Sbjct: 361 FELEPDEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLM----------HGGSSDEKKEV 420

Query: 421 VQVMDSESLAKLLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKE 480
            Q MDSE+LA LLSSLL+N+D Q+EM+ ML+   G++ ++DLEM K+IK+LE ELA+EKE
Sbjct: 421 DQAMDSETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKE 480

Query: 481 RSFQLELDYEVLKLKLPDSEEEEDDSD 497
           +SFQLEL++E+LKLKLPDS+EE++  +
Sbjct: 481 KSFQLELNFELLKLKLPDSDEEQEQEE 491

BLAST of Tan0015614 vs. NCBI nr
Match: KAG7013499.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 600.1 bits (1546), Expect = 1.7e-167
Identity = 317/507 (62.52%), Postives = 390/507 (76.92%), Query Frame = 0

Query: 1   MEHPQFQDQETTAAGR------IRFQFPTTADGYKILSQIGVSDHSTVYMAICKSADS-T 60
           M+  Q QDQET+AA           +FP  AD Y+I SQIG SDHSTVY AICKSAD+ T
Sbjct: 1   MDRRQIQDQETSAASSASAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPT 60

Query: 61  IVAVKIINIDIDIDPISIMDIK-SLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQ 120
           IVA+KII+ +++ID  +I +I  +LS INHP+IL+PHCCFTA DGRLWIV+PFIP+GPLQ
Sbjct: 61  IVAIKIIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQ 120

Query: 121 AIVSNRFPSGLPDQFMAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSL 180
           AI+S RF +GLPDQFM+IL+NQLL  LV LHE G VHKA+KASNIFFD+ G V LSDL +
Sbjct: 121 AIISQRFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDV 180

Query: 181 SNHTGDDNDHDDDRFSVPYWAPPEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQM 240
           S       +   D  SVPYW PPE++YSFKSDVWS+GILAMEIAYG PPI   +++K+Q 
Sbjct: 181 S------KEMKKDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQF 240

Query: 241 KNISKRFPYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKG 300
             IS+RFPYDLNLD +SGKSV+ RKFGD  K FV SCL KDRFAR SA SLL HPF+KKG
Sbjct: 241 SKISRRFPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKG 300

Query: 301 ESSNFLFKSIAKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQ-NEKITKISGWDYNNFL 360
           E+SNFLFKSIAKG+VGIG+ FEQDKDRI+ LMKNE++L   SQ   K+ +I+GWDYNNFL
Sbjct: 301 ENSNFLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFL 360

Query: 361 FELEPDEESLGKRVRFCGETIIPFREEE--IKFDMPLFLQRMDSLSGRTNGSQNEDKKNK 420
           FELEPDEES GKRVRF GET+  ++E+E   KFDM   +           GS +E KK  
Sbjct: 361 FELEPDEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLM--------HGGGSSDEKKKEV 420

Query: 421 VQVMDSESLAKLLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKE 480
            Q MD+E+LA LLSSLL+N+D Q+EM+ ML+   G++ ++DLEM K+IK+LE ELA+EKE
Sbjct: 421 DQAMDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKE 480

Query: 481 RSFQLELDYEVLKLKLPDSEEEEDDSD 497
           +SFQLEL++E+LKLKLPDS+EE++  +
Sbjct: 481 KSFQLELNFELLKLKLPDSDEEQEQEE 493

BLAST of Tan0015614 vs. ExPASy TrEMBL
Match: A0A6J1CA92 (serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC111009741 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 1.2e-179
Identity = 338/505 (66.93%), Postives = 415/505 (82.18%), Query Frame = 0

Query: 5   QFQDQE-TTAAGRIRFQFPTTADGYKILSQIGVSDHSTVYMAICKSA-DSTIVAVKIINI 64
           Q QD E   AAGR+ FQFPT ADGYKIL QIGVSDHS VY AIC+SA +S  VAVK+I++
Sbjct: 6   QDQDPENAAAAGRLHFQFPTAADGYKILYQIGVSDHSVVYKAICESANNSAKVAVKVIDV 65

Query: 65  DIDIDPISIMDIKSLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSG 124
           D  +D   +M + SLSLI+H NILSPHCCFT  D RLW V+PFI +GPLQAI++NRFP+G
Sbjct: 66  DRHVDEFFVMKMNSLSLIDHANILSPHCCFTV-DRRLWTVMPFIHAGPLQAILANRFPNG 125

Query: 125 LPDQFMAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGDDNDH 184
           LPDQF+AILL+QLL  L+CLHE+GYVH+ IKASNIF DS+GSV+LSDL++  + GDD   
Sbjct: 126 LPDQFLAILLHQLLRSLICLHELGYVHRNIKASNIFVDSEGSVKLSDLNV--YVGDD--- 185

Query: 185 DDDRFSVPYWAPPEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKRFPYD 244
           DDD  SVPYW PP++EYSFKSDVW+IGI+A+E+AYG PPIS SR ++SQ+ NIS+RFPY+
Sbjct: 186 DDDDSSVPYWDPPDEEYSFKSDVWAIGIVALEVAYGCPPISDSRTIRSQLLNISRRFPYN 245

Query: 245 LNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFLFKSI 304
           LNLDK S KSVIDRKFG+LFKDFVSSCL+KD   RPSA+SLLAHPFIKKGE+SN LFKSI
Sbjct: 246 LNLDKMSSKSVIDRKFGELFKDFVSSCLVKDPSKRPSAASLLAHPFIKKGETSNVLFKSI 305

Query: 305 AKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQNEKITKISGWDYNNFLFELEPDEESLG 364
           AK + GI DRFE++KDR++ LMK + ++E+ ++   + K+SGWD+NNFLFELEPDEESLG
Sbjct: 306 AKDMAGIEDRFEKNKDRLVELMKKDRRVEDNNRG-NVVKVSGWDFNNFLFELEPDEESLG 365

Query: 365 KRVRFCGETIIPFREEEI---------KFDMPLFLQRMDSLS-GRTNGSQNEDKKNKVQV 424
           KRVRF GETIIP R++ +         KF++PL L+RMDSLS    +G      + +V+V
Sbjct: 366 KRVRFRGETIIPLRDDHVSLNEAGEASKFNVPLLLKRMDSLSLSGLSGRMGSRAEEEVRV 425

Query: 425 MDSESLAKLLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKERSF 484
           MDSE+LAK+L+SL+E++D QRE+IMM+I+QFG L ERD+EMTKEIKKL+AELA EKERSF
Sbjct: 426 MDSETLAKVLTSLVESIDQQREVIMMVISQFGRLLERDMEMTKEIKKLKAELAYEKERSF 485

Query: 485 QLELDYEVLKLKLPDSEEEEDDSDN 498
           QLELDYEVLKLKLPDS E+ED S+N
Sbjct: 486 QLELDYEVLKLKLPDS-EDEDKSEN 502

BLAST of Tan0015614 vs. ExPASy TrEMBL
Match: A0A6J1H6C5 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460018 PE=4 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.5e-169
Identity = 317/501 (63.27%), Postives = 390/501 (77.84%), Query Frame = 0

Query: 1   MEHPQFQDQETTAAGRIRFQFPTTADGYKILSQIGVSDHSTVYMAICKSADS-TIVAVKI 60
           M+  Q QDQET+AA     +FP  AD Y+I SQIG SDHSTVY AICKSAD+ TIVA+KI
Sbjct: 1   MDRRQIQDQETSAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKI 60

Query: 61  INIDIDIDPISIMDIK-SLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNR 120
           I+ +++ID  +I +I  +LS INHP+IL+PHCCFTA DGRLWIV+PFIP+GPLQAI+S R
Sbjct: 61  IDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQR 120

Query: 121 FPSGLPDQFMAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGD 180
           F +GLPDQFM+IL+NQLL  LV LHE G VHKA+KASNIFFD+ G V LSDL +S     
Sbjct: 121 FRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVS----- 180

Query: 181 DNDHDDDRFSVPYWAPPEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKR 240
             +   D  SVPYW PPE++YSFKSDVWS+GILAMEIAYG PPI   +++K+Q   IS+R
Sbjct: 181 -KEMKKDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRR 240

Query: 241 FPYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFL 300
           FPYDLNLD +SGKSV+ RKFGD  K FV SCL KDRFAR SA SLL HPF+KKGE+SNFL
Sbjct: 241 FPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENSNFL 300

Query: 301 FKSIAKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQ-NEKITKISGWDYNNFLFELEPD 360
           FKSIAKG+VGIG+ FEQDKDRI+ LMKNE++L   SQ   K+ +I+GWDYNNFLFELEPD
Sbjct: 301 FKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPD 360

Query: 361 EESLGKRVRFCGETIIPFREEE--IKFDMPLFLQRMDSLSGRTNGSQNEDKKNKVQVMDS 420
           EES GKRVRF GET+  ++E+E   KFDM   +           GS +E KK   Q MD+
Sbjct: 361 EESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMH---------GGSSDEKKKEVDQAMDT 420

Query: 421 ESLAKLLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKERSFQLE 480
           E+LA LLSSLL+N+D Q+EM+ ML+   G++ ++DLEM K+IK+LE ELA+EKE+SFQLE
Sbjct: 421 ETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLE 480

Query: 481 LDYEVLKLKLPDSEEEEDDSD 497
           L++E+LKLKLPDS+EE++  +
Sbjct: 481 LNFELLKLKLPDSDEEQEQEE 486

BLAST of Tan0015614 vs. ExPASy TrEMBL
Match: A0A6J1KYZ4 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498878 PE=4 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 9.1e-167
Identity = 314/503 (62.43%), Postives = 388/503 (77.14%), Query Frame = 0

Query: 1   MEHPQFQDQETTAAGRIRFQFPTTADGYKILSQIGVSDHSTVYMAICKSADST-IVAVKI 60
           M+  Q QDQET+AA    F+FP  AD Y+I SQIG SDHSTVY AICKSADST IVA+KI
Sbjct: 1   MDRRQIQDQETSAAKGTHFKFPKVADEYEIQSQIGASDHSTVYKAICKSADSTMIVAIKI 60

Query: 61  INIDIDIDPISIMDIK-SLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNR 120
           I+ +++IDP +I +I  +LS INHP+ILSPHCCF A+DG LWIV+PFI +GPLQAI+S R
Sbjct: 61  IDSNVEIDPTAITNINTNLSFINHPHILSPHCCFIANDGCLWIVMPFIHAGPLQAIISQR 120

Query: 121 FPSGLPDQFMAILLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGD 180
           F +GLPDQFM+IL+NQLL  LV LHE G VHK++KASNIFFD+ G V LSDL +S     
Sbjct: 121 FRTGLPDQFMSILINQLLRALVFLHEKGLVHKSVKASNIFFDTHGCVLLSDLDVS----- 180

Query: 181 DNDHDDDRFSVPYWAPPEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKR 240
             D + D  SVPYW PPE++YS KSDVWS+GILAMEIAYG PPI   +++K+Q   IS+R
Sbjct: 181 -KDMNKDCSSVPYWVPPEEDYSSKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRR 240

Query: 241 FPYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFL 300
           FPYDLNLD +SGKSV+ RKFGD  K FV SCL KD FAR SA SLL HPF+KKGE+SNFL
Sbjct: 241 FPYDLNLDTRSGKSVVPRKFGDTLKHFVGSCLAKDPFARVSALSLLDHPFVKKGENSNFL 300

Query: 301 FKSIAKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQ---NEKITKISGWDYNNFLFELE 360
           FKSIAKG+VGIG+ FEQDKDRI+ LMKNE++L   SQ     K+ +I+GWDYNNFLFELE
Sbjct: 301 FKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGGGKVLRITGWDYNNFLFELE 360

Query: 361 PDEESLGKRVRFCGETIIPFREEE--IKFDMPLFLQRMDSLSGRTNGSQNEDKKNKVQVM 420
           PDEES GKRVRF GET+  ++E+E   KFDM   +              +++KK   Q M
Sbjct: 361 PDEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMH-----------GGSDEKKEVDQAM 420

Query: 421 DSESLAKLLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKERSFQ 480
           DSE+LA LLSSLL+N+D Q+EM+ ML+   G++ ++DLEM K+IK+LE ELA+EKE+SFQ
Sbjct: 421 DSETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQ 480

Query: 481 LELDYEVLKLKLPDSEEEEDDSD 497
           LEL++E+LKLKLPDS+EE++  +
Sbjct: 481 LELNFELLKLKLPDSDEEQEQEE 486

BLAST of Tan0015614 vs. ExPASy TrEMBL
Match: A0A5D3C8A9 (Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00250 PE=4 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 9.8e-137
Identity = 270/494 (54.66%), Postives = 355/494 (71.86%), Query Frame = 0

Query: 21  FPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINIDIDIDPISIMDIK----S 80
           FPTT+D Y IL+QIG S +S VY+A C S DS+  +   + I I   P  I D      S
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFDTNLLNTS 62

Query: 81  LSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSGLPDQFMAILLNQLL 140
           L+LI+HPNI+ PHCCF ADD RLWIV+P++ +GPLQ+++S+ FP G+PDQF +ILL+Q+L
Sbjct: 63  LTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQVL 122

Query: 141 HGLVCLHEMGYVHKAIKASNIFFDSQGSVQLS--DLSLSNHTGDDNDHDDDRFSVPYWAP 200
             LV LH MG+VHKAIK SNIFFDSQG+V+LS  DL +S     DN  D D  S+PYW P
Sbjct: 123 QALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMIS----ADNKIDRDS-SIPYWVP 182

Query: 201 PEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKRFPYDLNLDKKSGKSVI 260
           P+DEYSFKSD+W +G+LAME+ +GGPPI   RI++SQ+ NIS+RFPY+L  +     S  
Sbjct: 183 PDDEYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKTETGRRNSGD 242

Query: 261 DRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGE-SSNFLFKSIAKGVVGIGDRF 320
            R FGD  K+FV +CL+KDR  RP+A+SLL HPF+ KGE ++NFLFKSI KGV+ I  RF
Sbjct: 243 RRIFGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLFKSIPKGVIEIDRRF 302

Query: 321 EQDKDRIMGLMKNEMKLENYSQNEKITKISGWDYNNFLFELEPDEESLGKRVRFCGETII 380
           + +K+RI+G+M N+  L+N  Q+EKITKISGW YN+FLFELEPD ESLGKRVRF GETI 
Sbjct: 303 QLEKERIIGIMNNDEGLKN--QSEKITKISGWVYNDFLFELEPDGESLGKRVRFGGETIR 362

Query: 381 PFREEEIK----------FDMPLFLQRMDS---LSGRTNGSQNEDKKNKVQVMDSESLAK 440
            F +EE +           D P  L+ ++      G  +G  ++ KK K +++D ESL K
Sbjct: 363 EFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSKKKKKKKKIVDQESLVK 422

Query: 441 LLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKERSFQLELDYEV 495
           +L+S+L N+D QREM+ MLI   GYLSE+  EM+ EIK+LE EL +EKE+ FQLEL+YE 
Sbjct: 423 MLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAEKEQYFQLELEYEF 482

BLAST of Tan0015614 vs. ExPASy TrEMBL
Match: A0A1S3CN88 (serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC103502862 PE=4 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 9.8e-137
Identity = 270/494 (54.66%), Postives = 355/494 (71.86%), Query Frame = 0

Query: 21  FPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINIDIDIDPISIMDIK----S 80
           FPTT+D Y IL+QIG S +S VY+A C S DS+  +   + I I   P  I D      S
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFDTNLLNTS 62

Query: 81  LSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSGLPDQFMAILLNQLL 140
           L+LI+HPNI+ PHCCF ADD RLWIV+P++ +GPLQ+++S+ FP G+PDQF +ILL+Q+L
Sbjct: 63  LTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQVL 122

Query: 141 HGLVCLHEMGYVHKAIKASNIFFDSQGSVQLS--DLSLSNHTGDDNDHDDDRFSVPYWAP 200
             LV LH MG+VHKAIK SNIFFDSQG+V+LS  DL +S     DN  D D  S+PYW P
Sbjct: 123 QALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMIS----ADNKIDRDS-SIPYWVP 182

Query: 201 PEDEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKRFPYDLNLDKKSGKSVI 260
           P+DEYSFKSD+W +G+LAME+ +GGPPI   RI++SQ+ NIS+RFPY+L  +     S  
Sbjct: 183 PDDEYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKTETGRRNSGD 242

Query: 261 DRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGE-SSNFLFKSIAKGVVGIGDRF 320
            R FGD  K+FV +CL+KDR  RP+A+SLL HPF+ KGE ++NFLFKSI KGV+ I  RF
Sbjct: 243 RRIFGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLFKSIPKGVIEIDRRF 302

Query: 321 EQDKDRIMGLMKNEMKLENYSQNEKITKISGWDYNNFLFELEPDEESLGKRVRFCGETII 380
           + +K+RI+G+M N+  L+N  Q+EKITKISGW YN+FLFELEPD ESLGKRVRF GETI 
Sbjct: 303 QLEKERIIGIMNNDEGLKN--QSEKITKISGWVYNDFLFELEPDGESLGKRVRFGGETIR 362

Query: 381 PFREEEIK----------FDMPLFLQRMDS---LSGRTNGSQNEDKKNKVQVMDSESLAK 440
            F +EE +           D P  L+ ++      G  +G  ++ KK K +++D ESL K
Sbjct: 363 EFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSKKKKKKKKIVDQESLVK 422

Query: 441 LLSSLLENMDHQREMIMMLITQFGYLSERDLEMTKEIKKLEAELASEKERSFQLELDYEV 495
           +L+S+L N+D QREM+ MLI   GYLSE+  EM+ EIK+LE EL +EKE+ FQLEL+YE 
Sbjct: 423 MLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAEKEQYFQLELEYEF 482

BLAST of Tan0015614 vs. TAIR 10
Match: AT4G14480.1 (Protein kinase superfamily protein )

HSP 1 Score: 228.8 bits (582), Expect = 9.6e-60
Identity = 159/502 (31.67%), Postives = 260/502 (51.79%), Query Frame = 0

Query: 16  RIRFQFPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINID---IDIDPISIM 75
           R + +FP  A+ Y+I+ +IGV   ++VY AIC   +S +VA+K I++D    D D +   
Sbjct: 3   RNKLEFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLDQSRADFDSLR-R 62

Query: 76  DIKSLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSGLPDQFMAILL 135
           + K++SL++HPNIL+ +C FT  D  LW+V+PF+  G L +IVS+ FPSGLP+  +++ L
Sbjct: 63  ETKTMSLLSHPNILNAYCSFTV-DRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFL 122

Query: 136 NQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSL----------SNHTGDDNDH 195
            + L+ +  LH+ G++H+ IKA NI  DS GSV+L+D  +          S+ T   +  
Sbjct: 123 KETLNAISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLR 182

Query: 196 DDDRFSVPYWAPPE-----DEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISK 255
             D    PYW  PE       Y FK+D+WS GI A+E+A+G PP+S    +KS +  I+K
Sbjct: 183 LTDIAGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITK 242

Query: 256 RF---PYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGES 315
           RF    Y++N    S K   ++KF   F++ V  CL +D   RPSA  LL HPF K  + 
Sbjct: 243 RFHFSDYEINTSGSSKKG--NKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKG 302

Query: 316 SNFLFKSIAKGVVGIGDRFEQDKDRIMGLMKNEMKLENYSQNEKITK---ISGWDYNNFL 375
            +F+ K++   +      F + +  I  +  ++   E   ++E+I K   ISGW++    
Sbjct: 303 LDFVVKNVLHSLSNAEQMFMESQILIKSVGDDDE--EEEEEDEEIVKNRRISGWNFREDD 362

Query: 376 FELEPDEESLGKRVRFCGETIIPFREEEIKFDMPLFLQRMDSLSGRTN----------GS 435
            +L P               + P  E +     P    +        N          G 
Sbjct: 363 LQLSP---------------VFPATESDSSESSPREEDQSKDKKEDDNVTITGYELGLGL 422

Query: 436 QNEDKKN---KVQVMDSESLAKLLSSLLENMDHQREMIMMLITQFGYLSE---RDLEMTK 478
            NE+ KN   +V   D + + + L  L ++++HQR  + ++I       E   R+ E+ +
Sbjct: 423 SNEEAKNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELLE 482

BLAST of Tan0015614 vs. TAIR 10
Match: AT5G14720.1 (Protein kinase superfamily protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.5e-52
Identity = 122/342 (35.67%), Postives = 191/342 (55.85%), Query Frame = 0

Query: 20  QFPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINID---IDIDPISIMDIKS 79
           +FP  A  YK+  +IG    +TV+ A+C    + +VA+K+++++    D+D I   ++++
Sbjct: 8   KFPLNAKDYKLYEEIGDGVSATVHRALCIPL-NVVVAIKVLDLEKCNNDLDGIR-REVQT 67

Query: 80  LSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSGLPDQFMAILLNQLL 139
           +SLINHPN+L  HC FT    +LW+V+P++  G    I+ + +P G  +  +A LL + L
Sbjct: 68  MSLINHPNVLQAHCSFTTGH-QLWVVMPYMAGGSCLHIIKSSYPDGFEEPVIATLLRETL 127

Query: 140 HGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGDDNDHDDDR---FSVPYWA 199
             LV LH  G++H+ +KA NI  DS G+V+L+D  +S    D  D    R      P W 
Sbjct: 128 KALVYLHAHGHIHRDVKAGNILLDSNGAVKLADFGVSACMFDTGDRQRSRNTFVGTPCWM 187

Query: 200 PPE-----DEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKRFPYDLNLDKK 259
            PE       Y FK+DVWS GI A+E+A+G  P S    +K  +  +    P    LD +
Sbjct: 188 APEVMQQLHGYDFKADVWSFGITALELAHGHAPFSKYPPMKVLLMTLQNAPP---GLDYE 247

Query: 260 SGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFLFKSIAKGVVG 319
                 D++F   FK+ V +CL+KD   RP++  LL HPF K    +++L K+I  G+  
Sbjct: 248 R-----DKRFSKAFKEMVGTCLVKDPKKRPTSEKLLKHPFFKHARPADYLVKTILNGLPP 307

Query: 320 IGDRFEQDKDRIMG-LMKNEMKLE-NYSQNEKITKISGWDYN 349
           +GDR+ Q K +    LM+N+ + E + SQ E I  IS W++N
Sbjct: 308 LGDRYRQIKSKEADLLMQNKSEYEAHLSQQEYIRGISAWNFN 338

BLAST of Tan0015614 vs. TAIR 10
Match: AT1G79640.1 (Protein kinase superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 3.3e-44
Identity = 108/344 (31.40%), Postives = 180/344 (52.33%), Query Frame = 0

Query: 20  QFPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINID---IDIDPISIMDIKS 79
           ++P   + Y +   IG    + V+ A+C   D  +VA+KI++ +    D++ IS  + ++
Sbjct: 8   KYPIGPEHYTLYEFIGQGVSALVHRALCIPFDE-VVAIKILDFERDNCDLNNIS-REAQT 67

Query: 80  LSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSGLPDQFMAILLNQLL 139
           + L++HPN+L  HC F +D   LW+++P++  G    I+   +P G  +  +A +L + L
Sbjct: 68  MMLVDHPNVLKSHCSFVSDH-NLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREAL 127

Query: 140 HGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGDDNDHDDDR---FSVPYWA 199
            GL  LH+ G++H+ +KA NI   ++G+V+L D  +S    D  D    R      P W 
Sbjct: 128 KGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNTFVGTPCWM 187

Query: 200 PPE-----DEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKRFPYDLNLDKK 259
            PE       Y FK+D+WS GI  +E+A+G  P S    +K  +  +    P    LD +
Sbjct: 188 APEVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQNAPP---GLDYE 247

Query: 260 SGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFLFKSIAKGVVG 319
                 D+KF   FK  ++SCL+KD   RPSA  LL H F K+  SS+++ + +  G+  
Sbjct: 248 R-----DKKFSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQARSSDYIARKLLDGLPD 307

Query: 320 IGDRFEQDKDRIMGLMKNEM----KLENYSQNEKITKISGWDYN 349
           + +R +  K +   ++  E     + E  SQNE    ISGW++N
Sbjct: 308 LVNRVQAIKRKEEDMLAQEKMADGEKEELSQNEYKRGISGWNFN 340

BLAST of Tan0015614 vs. TAIR 10
Match: AT1G79640.2 (Protein kinase superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 3.3e-44
Identity = 108/344 (31.40%), Postives = 180/344 (52.33%), Query Frame = 0

Query: 20  QFPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINID---IDIDPISIMDIKS 79
           ++P   + Y +   IG    + V+ A+C   D  +VA+KI++ +    D++ IS  + ++
Sbjct: 5   KYPIGPEHYTLYEFIGQGVSALVHRALCIPFDE-VVAIKILDFERDNCDLNNIS-REAQT 64

Query: 80  LSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSGLPDQFMAILLNQLL 139
           + L++HPN+L  HC F +D   LW+++P++  G    I+   +P G  +  +A +L + L
Sbjct: 65  MMLVDHPNVLKSHCSFVSDH-NLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREAL 124

Query: 140 HGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGDDNDHDDDR---FSVPYWA 199
            GL  LH+ G++H+ +KA NI   ++G+V+L D  +S    D  D    R      P W 
Sbjct: 125 KGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNTFVGTPCWM 184

Query: 200 PPE-----DEYSFKSDVWSIGILAMEIAYGGPPISSSRIVKSQMKNISKRFPYDLNLDKK 259
            PE       Y FK+D+WS GI  +E+A+G  P S    +K  +  +    P    LD +
Sbjct: 185 APEVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQNAPP---GLDYE 244

Query: 260 SGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFLFKSIAKGVVG 319
                 D+KF   FK  ++SCL+KD   RPSA  LL H F K+  SS+++ + +  G+  
Sbjct: 245 R-----DKKFSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQARSSDYIARKLLDGLPD 304

Query: 320 IGDRFEQDKDRIMGLMKNEM----KLENYSQNEKITKISGWDYN 349
           + +R +  K +   ++  E     + E  SQNE    ISGW++N
Sbjct: 305 LVNRVQAIKRKEEDMLAQEKMADGEKEELSQNEYKRGISGWNFN 337

BLAST of Tan0015614 vs. TAIR 10
Match: AT1G70430.1 (Protein kinase superfamily protein )

HSP 1 Score: 169.9 bits (429), Expect = 5.3e-42
Identity = 113/350 (32.29%), Postives = 180/350 (51.43%), Query Frame = 0

Query: 14  AGRIRFQFPTTADGYKILSQIGVSDHSTVYMAICKSADSTIVAVKIINID---IDIDPIS 73
           AG    +FP  A  Y++  ++G    +TVY A C  A + IVAVKI++++    D++ I 
Sbjct: 2   AGSSTKRFPLYAKDYELFEEVGEGVSATVYRARC-IALNEIVAVKILDLEKCRNDLETIR 61

Query: 74  IMDIKSLSLINHPNILSPHCCFTADDGRLWIVLPFIPSGPLQAIVSNRFPSGLPDQFMAI 133
             ++  +SLI+HPN+L  HC F  D   LWIV+P++  G    ++ + +P GL    +A 
Sbjct: 62  -KEVHIMSLIDHPNLLKAHCSF-IDSSSLWIVMPYMSGGSCFHLMKSVYPEGLEQPIIAT 121

Query: 134 LLNQLLHGLVCLHEMGYVHKAIKASNIFFDSQGSVQLSDLSLSNHTGDDNDHDDDR---F 193
           LL ++L  LV LH  G++H+ +KA NI   S+G V+L D  +S    D  +    R    
Sbjct: 122 LLREVLKALVYLHRQGHIHRDVKAGNILIHSKGVVKLGDFGVSACMFDSGERMQTRNTFV 181

Query: 194 SVPYWAPPE-----DEYSFKSDVWSIGILAMEIAYGGPPISS---SRIVKSQMKNISKRF 253
             P W  PE     D Y FK            +A+G  P S     +++   ++N   R 
Sbjct: 182 GTPCWMAPEVMQQLDGYDFK-----------YLAHGHAPFSKYPPMKVLLMTLQNAPPRL 241

Query: 254 PYDLNLDKKSGKSVIDRKFGDLFKDFVSSCLIKDRFARPSASSLLAHPFIKKGESSNFLF 313
            YD            D+KF   F++ +++CL+KD   RP+A+ LL HPF K   S+++L 
Sbjct: 242 DYDR-----------DKKFSKSFRELIAACLVKDPKKRPTAAKLLKHPFFKHARSTDYLS 301

Query: 314 KSIAKGVVGIGDRFEQDKDRIMGLMKN-EMKLENYSQNEKITKISGWDYN 349
           + I  G+  +G+RF++ K+    L K      E  SQ+E +  IS W+++
Sbjct: 302 RKILHGLSPLGERFKKLKEAEAELFKGINGDKEQLSQHEYMRGISAWNFD 326

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O233041.4e-5831.67Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 P... [more]
Q551H46.1e-3530.40Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fr... [more]
G5EEN42.0e-3030.35Germinal center kinase 3 OS=Caenorhabditis elegans OX=6239 GN=gck-3 PE=1 SV=1[more]
Q5R4952.3e-2929.26Serine/threonine-protein kinase OSR1 OS=Pongo abelii OX=9601 GN=OXSR1 PE=2 SV=1[more]
Q6P9R22.9e-2929.26Serine/threonine-protein kinase OSR1 OS=Mus musculus OX=10090 GN=Oxsr1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022138626.12.5e-17966.93serine/threonine-protein kinase BLUS1-like [Momordica charantia][more]
XP_022958864.13.1e-16963.27serine/threonine-protein kinase BLUS1-like [Cucurbita moschata][more]
KAG6574930.13.1e-16963.27Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023548299.11.3e-16762.33serine/threonine-protein kinase BLUS1-like [Cucurbita pepo subsp. pepo][more]
KAG7013499.11.7e-16762.52Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. ar... [more]
Match NameE-valueIdentityDescription
A0A6J1CA921.2e-17966.93serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1H6C51.5e-16963.27serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KYZ49.1e-16762.43serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5D3C8A99.8e-13754.66Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3CN889.8e-13754.66serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC1035028... [more]
Match NameE-valueIdentityDescription
AT4G14480.19.6e-6031.67Protein kinase superfamily protein [more]
AT5G14720.11.5e-5235.67Protein kinase superfamily protein [more]
AT1G79640.13.3e-4431.40Protein kinase superfamily protein [more]
AT1G79640.23.3e-4431.40Protein kinase superfamily protein [more]
AT1G70430.15.3e-4232.29Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 450..477
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 17..105
e-value: 2.3E-14
score: 55.2
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 117..305
e-value: 1.1E-33
score: 118.7
NoneNo IPR availablePANTHERPTHR48014SERINE/THREONINE-PROTEIN KINASE FRAY2coord: 16..492
NoneNo IPR availablePANTHERPTHR48014:SF7SERINE/THREONINE-PROTEIN KINASE BLUS1coord: 16..492
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 28..289
e-value: 7.5E-35
score: 120.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 28..289
score: 32.668636
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 23..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0015614.1Tan0015614.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity