Tan0015464 (gene) Snake gourd v1

Overview


Name	Tan0015464
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha
Location	LG06: 633071 .. 633918 (+)
RNA-Seq Expression	Tan0015464
Synteny	Tan0015464

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCTGAAATCTCAGATGATCAGATTATTGGTAAAGATTTAGAAGACCAAGACTACTTGGAGAAGATAACCAAGTTACAGGGTTACCTCGATGAGGTGAAAATTCTGCCTTTAAGTTTCTATCTCGAAATGAATCCGTATTTATACCTCCATTTGCCTTATGGTCTTTCAGTTTCTTTCTTGGGTTGGATTGTTTGCTGTATCATTTTGTTTTAAAGAAAAATTGGTGAAGTACTGGCTGACGTCGACGAATGTCATATGGCCTGGTTTATGATTCTAGCAACTGACTGAACTTGAGTGTGGAAAAATGAAAAAGAAAGAGAAAAGAATCCCTTCCTTACCATTCTTTTTGAAACTTATGGAGAATTTTGCTTCTCGTCTTCTCCCTCACTGGTCGGTTTCTGATTATATCTTTAAAATTAGACAGCTAATTCAGTTTTTTATTATCATTGTTCTTTTTAATGAAGATAGCCATACTTTCATAAAGCAAATGGAAAATAGACCACAACCAATGGAGCCAAAACAAAGTTCAGTTTAAATTTGACTGCATGATTGAATAACCTCTTGTTTCTAAGATCCTTGGTCTGTACAGTAAGCATATCTCCACATTCAAGCGGATGAAAGTTTTGCAATTTCTAAATATTAGTGTTTTTGCAGCTATATATTACTATTTTGATATCTGAGTTAACCTCAAGCTTGGCGGCTCATGCTCAATTTAGGTGAAGAAGATTGTGAGAGAAAGCTGTTCCCAAGAGGTTTTAGAAGTGGCTTTGAGCTCCGTCTCCTCCCTAATTATCATCCTCTCATCCATGTCATCGTCTACCAAACTCCACTCTTCACTTTGA

mRNA sequence

ATGGAAGCTGAAATCTCAGATGATCAGATTATTGGTAAAGATTTAGAAGACCAAGACTACTTGGAGAAGATAACCAAGTTACAGGGTTACCTCGATGAGGTGAAGAAGATTGTGAGAGAAAGCTGTTCCCAAGAGGTTTTAGAAGTGGCTTTGAGCTCCGTCTCCTCCCTAATTATCATCCTCTCATCCATGTCATCGTCTACCAAACTCCACTCTTCACTTTGA

Coding sequence (CDS)

ATGGAAGCTGAAATCTCAGATGATCAGATTATTGGTAAAGATTTAGAAGACCAAGACTACTTGGAGAAGATAACCAAGTTACAGGGTTACCTCGATGAGGTGAAGAAGATTGTGAGAGAAAGCTGTTCCCAAGAGGTTTTAGAAGTGGCTTTGAGCTCCGTCTCCTCCCTAATTATCATCCTCTCATCCATGTCATCGTCTACCAAACTCCACTCTTCACTTTGA

Protein sequence

MEAEISDDQIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSMSSSTKLHSSL

Homology

[Close]

BLAST of Tan0015464 vs. ExPASy Swiss-Prot
Match: Q9C9K3 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 OS=Arabidopsis thaliana OX=3702 GN=PFP-ALPHA2 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 5.2e-07
Identity = 30/63 (47.62%), Postives = 48/63 (76.19%), Query Frame = 0


Query: 6   SDDQIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSMS 65
           +D + +   +EDQDY+ KI KLQ YLD+V+ IV+  CSQ+VL+ ALS ++S+  +L+++S
Sbjct: 548 ADAKAVSLCVEDQDYMGKIKKLQEYLDQVRTIVKPGCSQDVLKAALSVMASVTDVLTTIS 607

Query: 66  SST 69
           SS+
Sbjct: 608 SSS 610

[Close]

BLAST of Tan0015464 vs. ExPASy Swiss-Prot
Match: Q41140 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha OS=Ricinus communis OX=3988 GN=PFP-ALPHA PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.2e-06
Identity = 29/54 (53.70%), Postives = 43/54 (79.63%), Query Frame = 0


Query: 15  LEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSMSSST 69
           +EDQDY+ +I KLQ YLD+V+ IV+  CSQEVL+ ALS ++S+  +L +MSS++
Sbjct: 557 VEDQDYMGRIKKLQEYLDKVRTIVKPGCSQEVLKAALSVMASVTDVLLTMSSTS 610

[Close]

BLAST of Tan0015464 vs. ExPASy Swiss-Prot
Match: P21342 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha OS=Solanum tuberosum OX=4113 GN=PFP-ALPHA PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 1.5e-06
Identity = 30/65 (46.15%), Postives = 48/65 (73.85%), Query Frame = 0


Query: 6   SDDQIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSMS 65
           +D + +   +EDQDY+ +I KLQ YLD+V+ IV+  CSQ+VL+ ALS+++S+  ILS +S
Sbjct: 548 ADAKAVSLCVEDQDYIGRIKKLQEYLDKVRTIVKPGCSQDVLKAALSAMASVTDILSVIS 607

Query: 66  SSTKL 71
           S + +
Sbjct: 608 SPSSV 612

[Close]

BLAST of Tan0015464 vs. ExPASy Swiss-Prot
Match: Q9SYP2 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PFP-ALPHA1 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.6e-06
Identity = 28/62 (45.16%), Postives = 47/62 (75.81%), Query Frame = 0


Query: 6   SDDQIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSMS 65
           +D + +   +EDQDY+ +I KLQ YLD+V+ IV+  CSQ+VL+ ALS ++S+  +L+++S
Sbjct: 550 ADAKAVSLCVEDQDYMGRIKKLQEYLDQVRTIVKPGCSQDVLKAALSVMASVTDVLTTIS 609

Query: 66  SS 68
           S+
Sbjct: 610 SN 611

[Close]

BLAST of Tan0015464 vs. NCBI nr
Match: KAG7025939.1 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 115.2 bits (287), Expect = 2.5e-22
Identity = 69/77 (89.61%), Postives = 71/77 (92.21%), Query Frame = 0


Query: 1  MEAEISDDQIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIII 60
          MEAEISDDQIIGK  EDQDYLEKITKLQGYLDEVKKIVRESCS+EVLEVALSSVSSLI I
Sbjct: 1  MEAEISDDQIIGKAFEDQDYLEKITKLQGYLDEVKKIVRESCSEEVLEVALSSVSSLISI 60

Query: 61 LSSMSSS---TKLHSSL 75
          LSSM+SS   TKLHSSL
Sbjct: 61 LSSMASSSSFTKLHSSL 77

[Close]

BLAST of Tan0015464 vs. NCBI nr
Match: KGN45427.1 (hypothetical protein Csa_016056 [Cucumis sativus])

HSP 1 Score: 107.8 bits (268), Expect = 4.0e-20
Identity = 64/75 (85.33%), Postives = 68/75 (90.67%), Query Frame = 0


Query: 1  MEAEISDD-QIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLII 60
          MEA++ DD QIIG  LEDQDYLEKITKLQGYLDEVKKIV ESCSQEVLE+AL S+SSLI 
Sbjct: 1  MEAKVLDDLQIIGTVLEDQDYLEKITKLQGYLDEVKKIVSESCSQEVLELALDSISSLIT 60

Query: 61 ILSSMSSSTKLHSSL 75
          ILSSMSSSTKLHSSL
Sbjct: 61 ILSSMSSSTKLHSSL 75

[Close]

BLAST of Tan0015464 vs. NCBI nr
Match: XP_016902860.1 (PREDICTED: pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2-like [Cucumis melo] >KAA0059347.1 pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 105.9 bits (263), Expect = 1.5e-19
Identity = 64/75 (85.33%), Postives = 68/75 (90.67%), Query Frame = 0


Query: 1  MEAEISDD-QIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLII 60
          MEA++  D QIIG  LEDQDYLEKITKLQGYLDEVKKIV ESCSQEVLEVAL+S+SSLI 
Sbjct: 1  MEAKVLVDLQIIGTVLEDQDYLEKITKLQGYLDEVKKIVSESCSQEVLEVALNSISSLIT 60

Query: 61 ILSSMSSSTKLHSSL 75
          ILSSMSSSTKLHSSL
Sbjct: 61 ILSSMSSSTKLHSSL 75

[Close]

BLAST of Tan0015464 vs. NCBI nr
Match: KAG6593594.1 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 91.7 bits (226), Expect = 2.9e-15
Identity = 56/63 (88.89%), Postives = 58/63 (92.06%), Query Frame = 0


Query: 15  LEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSM---SSSTKLH 74
           L +QDYLEKITKLQGYLDEVKKIVRESCS+EVLEVALSSVSSLI ILSSM   SSSTKLH
Sbjct: 359 LSNQDYLEKITKLQGYLDEVKKIVRESCSEEVLEVALSSVSSLISILSSMASSSSSTKLH 418

[Close]

BLAST of Tan0015464 vs. NCBI nr
Match: PKA60658.1 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha [Apostasia shenzhenica])

HSP 1 Score: 66.6 bits (161), Expect = 1.0e-07
Identity = 37/67 (55.22%), Postives = 52/67 (77.61%), Query Frame = 0


Query: 6   SDDQIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSMS 65
           SD + I   +EDQDY+ K+ +LQ Y+D+VKKIV+  CSQEVL+VALSS++S++ ILS MS
Sbjct: 456 SDSKTISLLVEDQDYMGKVKELQAYIDKVKKIVKPGCSQEVLKVALSSMASVVDILSVMS 515

Query: 66  SSTKLHS 73
             +K H+
Sbjct: 516 PPSKSHN 522

[Close]

BLAST of Tan0015464 vs. ExPASy TrEMBL
Match: A0A0A0KAL4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447890 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.9e-20
Identity = 64/75 (85.33%), Postives = 68/75 (90.67%), Query Frame = 0


Query: 1  MEAEISDD-QIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLII 60
          MEA++ DD QIIG  LEDQDYLEKITKLQGYLDEVKKIV ESCSQEVLE+AL S+SSLI 
Sbjct: 1  MEAKVLDDLQIIGTVLEDQDYLEKITKLQGYLDEVKKIVSESCSQEVLELALDSISSLIT 60

Query: 61 ILSSMSSSTKLHSSL 75
          ILSSMSSSTKLHSSL
Sbjct: 61 ILSSMSSSTKLHSSL 75

[Close]

BLAST of Tan0015464 vs. ExPASy TrEMBL
Match: A0A5A7UXI5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G00720 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 7.3e-20
Identity = 64/75 (85.33%), Postives = 68/75 (90.67%), Query Frame = 0


Query: 1  MEAEISDD-QIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLII 60
          MEA++  D QIIG  LEDQDYLEKITKLQGYLDEVKKIV ESCSQEVLEVAL+S+SSLI 
Sbjct: 1  MEAKVLVDLQIIGTVLEDQDYLEKITKLQGYLDEVKKIVSESCSQEVLEVALNSISSLIT 60

Query: 61 ILSSMSSSTKLHSSL 75
          ILSSMSSSTKLHSSL
Sbjct: 61 ILSSMSSSTKLHSSL 75

[Close]

BLAST of Tan0015464 vs. ExPASy TrEMBL
Match: A0A1S4E4F9 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2-like OS=Cucumis melo OX=3656 GN=LOC103500631 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 7.3e-20
Identity = 64/75 (85.33%), Postives = 68/75 (90.67%), Query Frame = 0


Query: 1  MEAEISDD-QIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLII 60
          MEA++  D QIIG  LEDQDYLEKITKLQGYLDEVKKIV ESCSQEVLEVAL+S+SSLI 
Sbjct: 1  MEAKVLVDLQIIGTVLEDQDYLEKITKLQGYLDEVKKIVSESCSQEVLEVALNSISSLIT 60

Query: 61 ILSSMSSSTKLHSSL 75
          ILSSMSSSTKLHSSL
Sbjct: 61 ILSSMSSSTKLHSSL 75

[Close]

BLAST of Tan0015464 vs. ExPASy TrEMBL
Match: A0A2I0AYQ0 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha OS=Apostasia shenzhenica OX=1088818 GN=PFP-ALPHA PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.9e-08
Identity = 37/67 (55.22%), Postives = 52/67 (77.61%), Query Frame = 0


Query: 6   SDDQIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSMS 65
           SD + I   +EDQDY+ K+ +LQ Y+D+VKKIV+  CSQEVL+VALSS++S++ ILS MS
Sbjct: 456 SDSKTISLLVEDQDYMGKVKELQAYIDKVKKIVKPGCSQEVLKVALSSMASVVDILSVMS 515

Query: 66  SSTKLHS 73
             +K H+
Sbjct: 516 PPSKSHN 522

[Close]

BLAST of Tan0015464 vs. ExPASy TrEMBL
Match: A0A5B7B4K3 (Uncharacterized protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_032996 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.1e-07
Identity = 39/63 (61.90%), Postives = 49/63 (77.78%), Query Frame = 0


Query: 12  GKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSMSSSTKLH 71
           G  L++ DY+ KI +LQ Y+D+VKKIV+  CSQ VLEVALSS+SSL+ +LS MSS  KL 
Sbjct: 130 GLCLQEHDYIGKIKELQVYIDKVKKIVKPGCSQVVLEVALSSMSSLVEVLSVMSSKQKLQ 189

Query: 72  SSL 75
           SSL
Sbjct: 190 SSL 192

[Close]

BLAST of Tan0015464 vs. TAIR 10
Match: AT1G76550.1 (Phosphofructokinase family protein )

HSP 1 Score: 54.7 bits (130), Expect = 3.7e-08
Identity = 30/63 (47.62%), Postives = 48/63 (76.19%), Query Frame = 0


Query: 6   SDDQIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSMS 65
           +D + +   +EDQDY+ KI KLQ YLD+V+ IV+  CSQ+VL+ ALS ++S+  +L+++S
Sbjct: 548 ADAKAVSLCVEDQDYMGKIKKLQEYLDQVRTIVKPGCSQDVLKAALSVMASVTDVLTTIS 607

Query: 66  SST 69
           SS+
Sbjct: 608 SSS 610

[Close]

BLAST of Tan0015464 vs. TAIR 10
Match: AT1G20950.1 (Phosphofructokinase family protein )

HSP 1 Score: 52.4 bits (124), Expect = 1.8e-07
Identity = 28/62 (45.16%), Postives = 47/62 (75.81%), Query Frame = 0


Query: 6   SDDQIIGKDLEDQDYLEKITKLQGYLDEVKKIVRESCSQEVLEVALSSVSSLIIILSSMS 65
           +D + +   +EDQDY+ +I KLQ YLD+V+ IV+  CSQ+VL+ ALS ++S+  +L+++S
Sbjct: 550 ADAKAVSLCVEDQDYMGRIKKLQEYLDQVRTIVKPGCSQDVLKAALSVMASVTDVLTTIS 609

Query: 66  SS 68
           S+
Sbjct: 610 SN 611

The following BLAST results are available for this feature:

BLAST of Tan0015464 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C9K3	5.2e-07	47.62	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 OS=Arab...	[more]
Q41140	1.2e-06	53.70	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha OS=Ricinu...	[more]
P21342	1.5e-06	46.15	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha OS=Solanu...	[more]
Q9SYP2	2.6e-06	45.16	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 OS=Arab...	[more]

BLAST of Tan0015464 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025939.1	2.5e-22	89.61	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha [Cucurbit...	[more]
KGN45427.1	4.0e-20	85.33	hypothetical protein Csa_016056 [Cucumis sativus]	[more]
XP_016902860.1	1.5e-19	85.33	PREDICTED: pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alph...	[more]
KAG6593594.1	2.9e-15	88.89	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha, partial ...	[more]
PKA60658.1	1.0e-07	55.22	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha [Apostasi...	[more]

BLAST of Tan0015464 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KAL4	1.9e-20	85.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447890 PE=4 SV=1	[more]
A0A5A7UXI5	7.3e-20	85.33	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2-like OS...	[more]
A0A1S4E4F9	7.3e-20	85.33	pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2-like OS...	[more]
A0A2I0AYQ0	4.9e-08	55.22	Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha OS=Aposta...	[more]
A0A5B7B4K3	1.1e-07	61.90	Uncharacterized protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_032996...	[more]

BLAST of Tan0015464 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76550.1	3.7e-08	47.62	Phosphofructokinase family protein	[more]
AT1G20950.1	1.8e-07	45.16	Phosphofructokinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR43650:SF14	PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE SUBUNIT ALPHA	coord: 2..72
None	No IPR available	PANTHER	PTHR43650	PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE	coord: 2..72

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0015464.1	Tan0015464.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046835	carbohydrate phosphorylation
biological_process	GO:0006002	fructose 6-phosphate metabolic process
biological_process	GO:0061615	glycolytic process through fructose-6-phosphate
biological_process	GO:0015979	photosynthesis
biological_process	GO:0009749	response to glucose
cellular_component	GO:0005829	cytosol
molecular_function	GO:0003872	6-phosphofructokinase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0047334	diphosphate-fructose-6-phosphate 1-phosphotransferase activity
molecular_function	GO:0046872	metal ion binding