Tan0015367 (gene) Snake gourd v1

Overview
NameTan0015367
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionNon-structural maintenance of chromosomes element 4
LocationLG09: 60442198 .. 60444676 (+)
RNA-Seq ExpressionTan0015367
SyntenyTan0015367
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGCCTGTCAAACGCGAACCCGCCGCCAGCAGCCGCAGTAATCAGAGTAAAGCCGGCGATGCCGTCGATGCTGCCAGTTTAAGGGAGGTTAAGCGTGAGAAGGTTGCTCGGGAGCGAGACAACTCCGGCGAGGACAACGCGACTGAAGAAGAAGAGGGTCAGCAAGACTCGACGAAGCGGCGGGTTCTTCGGTCTCGGTACCTTGCGGTCATTCATGAGATTAGTGGTATGATGAATCTCTTCCTTTTGTTTTACTACTGGATTTAAGTTGTGGGTTTGATTTGTTTGTGTGGGTAATCGTTTTCTTTTTTGGTTTCTTGTCTTTGAAGAAAGGAGAGAGGATTTGTCGAAGGATTCGGATAAGTTCAATGTTATCATCAACGAGGTGGAAAAGTTGCACGAGCAAGGTATTTCTATAGTGTTTCTTTTCTCCTTGTTTACGTTGAAGAACTGTTCTCTCTTTTATTTAAATTTTTCATCAAAAGAAAAGTAAAATACAAGCGAAAATTGATACTTGTCCGTTGTTGCCTTTTCCCCTTGCATTCTTATATTCATATAAGTTTGAATGTGTATTGAACTTCGAGTTATGAAAGGGGATTTGTTATTTTTCCTTTTGGTATTGTTACTCATATGACATGGTTGTATTTTCATTTGGGTTTCAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCGTTGCAAGATATTGCAAACTCCCTTGTGACCTCTATTAGGTCCCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTGAGTTGTCTGCTCAGAGAATTTGCCGACATTAATGGTGGTATAACATCTGAAGAAAACAATCTGATTTCTCTCAGTTGGAAAGATATTGGACTAAGTGTTTCGTCTATTTTCATGAATGGCCATGGATGCTGCACCATGTGAGATTCATAGATTCCTTTTTCTCTCTAACTTAATGCTAAAGTGGGTTCAATGGACGTTTTTTTTCCTTAATAAATTGCATAACTTCCTTTGTTGTGTGTTCTTTTTTAAAGGCTAGGACCTATGAGTACTCAGTTGAAGCAGCGAAAGACTAATATTTCCAGGAAACGTTCCAGGCCTACTGAAAGTTCGAGACCTGCAGAGGTTAGACTGTAAAGATTTTGATATTTAAGCATTTTAGGATTGAGTCATCTGTCACTCTGTTCTGAGCTTTTTTAACTTGTTGCTAAGAGTCTGTGACCTACAGAAGAAGTTGCTTTAAAATTCCATGCTACAGCCAACTTTGTAAGCACTCTATTTATTTGGAGATTAGTATAAGCGTGTTTTGGATTACTCTGTTAGATGGCATCGTGCCCAATTTTACCGAAAGCACAGATATTTCTTTTTTGGTAGGACATATATTATTGAATTTGCTAATATTTCAGCTGGACATTATAAAGCTTGTTTGTGAGAGTCACTCCTCAAGCTTAGTGCTTGATGTTCTTTGCTCGTACCCATAGGCTACAACTTTGTTCTGTTTGCTCAAGTGGCTTGTAGAATTAATTGCATTTTCTGTTTGTTTTTCATGCATTTGATATTCTATTACCCCGTTGATGTTTATTTCAATATTCATACGTGCAGGTTAATGAAAATGGATCTGTAGAAAAAACTGATACAGATAAGAACATGAGCATAATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAAACTTGAACATTTGGTATTGAATAGAAAGTCTTTTGCACAGACGGTAGAGAACTTATTTGCTCTATCCTTTCTGGTTAAGGATGGTCGGGCTGAGATCACTATTGATAAAAATGGTTCCCATTTTGTATGTGAGTAAACATCTACAGACTAAACTTTTTTATGCTTTTCTTTAAAAACATTGTGTTCATTGCATTATAAACTTACATTTTCTGTCAACATTTTCAGCACCGAAGAATGCCCCTGCCCATAGTGCCATTATGTCACATGAAGTTAATTACAGTCATTTTGTATTCAGATTTGATTTCAAGGATTGGAAGGTACAGAGTGGCATTCATTGTTATGATACCGACTTTGCATTTTGCAGTGTCATGTAACAGAGTTCAAATATTAAGAAAAATATTCTACCCTCTTGATTTGTAGATACAATATTGGATAGAGGCTCAAAATATATATATATGAAATGTTGTGTACTGTTTAGATCCTTGGCTTCAATTGAGGAACACATACTTGTGATATTTATTTGCAGCTGATGATGGATATGGTACCAGTTGGAGAGGAGCTGATGCCCCACAGGATCTTTCTGAATTCAGCATCGATGCCCCAAGCAGAAGCATCAGCAGACAATTCTGAAGCAGCAACATTGCCTACCACACCAATTCGAAAGCTGTCAAGAAATCGAGGTTTAGTTATGCAATCTGTTGTTGAGGACTCCCCTGATAACGGCGCTCATTCAGGACCAACTGCAATTTTGAGGTGCAAGAGAAAGCTTAGTCAAGGTTTAGAAAATTAA

mRNA sequence

ATGAGGCCTGTCAAACGCGAACCCGCCGCCAGCAGCCGCAGTAATCAGAGTAAAGCCGGCGATGCCGTCGATGCTGCCAGTTTAAGGGAGGTTAAGCGTGAGAAGGTTGCTCGGGAGCGAGACAACTCCGGCGAGGACAACGCGACTGAAGAAGAAGAGGGTCAGCAAGACTCGACGAAGCGGCGGGTTCTTCGGTCTCGGTACCTTGCGGTCATTCATGAGATTAGTGAAAGGAGAGAGGATTTGTCGAAGGATTCGGATAAGTTCAATGTTATCATCAACGAGGTGGAAAAGTTGCACGAGCAAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCGTTGCAAGATATTGCAAACTCCCTTGTGACCTCTATTAGGTCCCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTGAGTTGTCTGCTCAGAGAATTTGCCGACATTAATGGTGGTATAACATCTGAAGAAAACAATCTGATTTCTCTCAGTTGGAAAGATATTGGACTAAGTGTTTCGTCTATTTTCATGAATGGCCATGGATGCTGCACCATGCTAGGACCTATGAGTACTCAGTTGAAGCAGCGAAAGACTAATATTTCCAGGAAACGTTCCAGGCCTACTGAAAGTTCGAGACCTGCAGAGGTTAATGAAAATGGATCTGTAGAAAAAACTGATACAGATAAGAACATGAGCATAATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAAACTTGAACATTTGGTATTGAATAGAAAGTCTTTTGCACAGACGGTAGAGAACTTATTTGCTCTATCCTTTCTGGTTAAGGATGGTCGGGCTGAGATCACTATTGATAAAAATGGTTCCCATTTTGTATCACCGAAGAATGCCCCTGCCCATAGTGCCATTATGTCACATGAAGTTAATTACAGTCATTTTGTATTCAGATTTGATTTCAAGGATTGGAAGCTGATGATGGATATGGTACCAGTTGGAGAGGAGCTGATGCCCCACAGGATCTTTCTGAATTCAGCATCGATGCCCCAAGCAGAAGCATCAGCAGACAATTCTGAAGCAGCAACATTGCCTACCACACCAATTCGAAAGCTGTCAAGAAATCGAGGTTTAGTTATGCAATCTGTTGTTGAGGACTCCCCTGATAACGGCGCTCATTCAGGACCAACTGCAATTTTGAGGTGCAAGAGAAAGCTTAGTCAAGGTTTAGAAAATTAA

Coding sequence (CDS)

ATGAGGCCTGTCAAACGCGAACCCGCCGCCAGCAGCCGCAGTAATCAGAGTAAAGCCGGCGATGCCGTCGATGCTGCCAGTTTAAGGGAGGTTAAGCGTGAGAAGGTTGCTCGGGAGCGAGACAACTCCGGCGAGGACAACGCGACTGAAGAAGAAGAGGGTCAGCAAGACTCGACGAAGCGGCGGGTTCTTCGGTCTCGGTACCTTGCGGTCATTCATGAGATTAGTGAAAGGAGAGAGGATTTGTCGAAGGATTCGGATAAGTTCAATGTTATCATCAACGAGGTGGAAAAGTTGCACGAGCAAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCGTTGCAAGATATTGCAAACTCCCTTGTGACCTCTATTAGGTCCCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTGAGTTGTCTGCTCAGAGAATTTGCCGACATTAATGGTGGTATAACATCTGAAGAAAACAATCTGATTTCTCTCAGTTGGAAAGATATTGGACTAAGTGTTTCGTCTATTTTCATGAATGGCCATGGATGCTGCACCATGCTAGGACCTATGAGTACTCAGTTGAAGCAGCGAAAGACTAATATTTCCAGGAAACGTTCCAGGCCTACTGAAAGTTCGAGACCTGCAGAGGTTAATGAAAATGGATCTGTAGAAAAAACTGATACAGATAAGAACATGAGCATAATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAAACTTGAACATTTGGTATTGAATAGAAAGTCTTTTGCACAGACGGTAGAGAACTTATTTGCTCTATCCTTTCTGGTTAAGGATGGTCGGGCTGAGATCACTATTGATAAAAATGGTTCCCATTTTGTATCACCGAAGAATGCCCCTGCCCATAGTGCCATTATGTCACATGAAGTTAATTACAGTCATTTTGTATTCAGATTTGATTTCAAGGATTGGAAGCTGATGATGGATATGGTACCAGTTGGAGAGGAGCTGATGCCCCACAGGATCTTTCTGAATTCAGCATCGATGCCCCAAGCAGAAGCATCAGCAGACAATTCTGAAGCAGCAACATTGCCTACCACACCAATTCGAAAGCTGTCAAGAAATCGAGGTTTAGTTATGCAATCTGTTGTTGAGGACTCCCCTGATAACGGCGCTCATTCAGGACCAACTGCAATTTTGAGGTGCAAGAGAAAGCTTAGTCAAGGTTTAGAAAATTAA

Protein sequence

MRPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGEDNATEEEEGQQDSTKRRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMNGHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGILRRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLPTTPIRKLSRNRGLVMQSVVEDSPDNGAHSGPTAILRCKRKLSQGLEN
Homology
BLAST of Tan0015367 vs. ExPASy Swiss-Prot
Match: Q9C689 (Non-structural maintenance of chromosomes element 4 homolog A OS=Arabidopsis thaliana OX=3702 GN=NSE4A PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.0e-104
Identity = 222/406 (54.68%), Postives = 290/406 (71.43%), Query Frame = 0

Query: 2   RPVKREPAASSRSNQSKAGDAVDAASLREVKREKVAR-ERDNSGEDNATEEEEGQQDSTK 61
           + VKRE  A+    + +A D  +   LR VK+EK  + E D+   D     +E +Q  + 
Sbjct: 3   KTVKRESEAT--GGKREADD--EPEKLRSVKKEKQRKTEADSVRPDEPPPPQEEEQGISD 62

Query: 62  RRVLRSRYLAVIHEISERREDLSK-DSDKFNVIINEVEKLHEQVQKPREQVADAEALQDI 121
           RR+LRS+YL++ +EI++ ++DL K DSDKF+ IIN VE LH+QV+KPREQ+ADAEAL DI
Sbjct: 63  RRILRSKYLSLQNEINDCKDDLMKIDSDKFSRIINAVENLHQQVRKPREQIADAEALLDI 122

Query: 122 ANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFM 181
           AN+L++S++SQS  GV+P++FV+ L+  F   + GI ++E   +SL WKD+G +V S  +
Sbjct: 123 ANTLMSSVKSQSAHGVSPAEFVNALISGFGQGSLGIDTDETAQVSLKWKDLGFAVCSTVL 182

Query: 182 NGHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGI 241
              GC TMLGPM T+LKQRK   +RKR++P E  RP EV+++ S EKTDTDKNM+IMF I
Sbjct: 183 VSCGCSTMLGPMDTELKQRKRAPNRKRTKPGEGVRPDEVDDSQSEEKTDTDKNMTIMFNI 242

Query: 242 LRRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSA 301
           L + K V+LE+LVLNR+SFAQTVENLFALSFL KDGR EI +DK+GSHF  P+NAP  + 
Sbjct: 243 LGKKKRVQLENLVLNRRSFAQTVENLFALSFLAKDGRVEIIVDKSGSHFAMPRNAPDANV 302

Query: 302 IMSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHR-IFLNSASMPQAEASADNSEAAT 361
           +MS EV Y+HFVFR DFKDWKLM +MVP+GEELMPHR   + S+S P A A A       
Sbjct: 303 VMSGEVIYNHFVFRLDFKDWKLMSEMVPLGEELMPHRQTAVASSSCPAASAPASADFTQD 362

Query: 362 LPTTPIRKLSRNRGLVMQ--SVVEDSPDNGAHSGPTAILRCKRKLS 403
             TTPIRKLSRNRGLV+Q  +VVED+PD     G     RCKR+L+
Sbjct: 363 TQTTPIRKLSRNRGLVVQEETVVEDTPDK---EGDGTRRRCKRRLT 401

BLAST of Tan0015367 vs. ExPASy Swiss-Prot
Match: F4JET1 (Non-structural maintenance of chromosomes element 4 homolog B OS=Arabidopsis thaliana OX=3702 GN=NSE4B PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.1e-85
Identity = 183/376 (48.67%), Postives = 260/376 (69.15%), Query Frame = 0

Query: 13  RSNQSKAGDAVDAASL-REVKREKVARERDNSGEDNATEEEEGQQDSTKRRVLRSRYLAV 72
           RS + +A ++ +   + ++ KR K         +    EEEE  Q    RR +RS+YLA+
Sbjct: 15  RSRRREADESSETPKIVKKEKRSKTVIASLRPQQPPIQEEEE--QGIADRRAIRSQYLAL 74

Query: 73  IHEISERREDLSK-DSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQ 132
            H+I + ++DL+K DS+KFN IINEVE LH++V+KPREQ+ADAEAL D+ NS+V+S++SQ
Sbjct: 75  THKIKDAKDDLTKIDSNKFNSIINEVENLHQKVRKPREQIADAEALLDLTNSVVSSVKSQ 134

Query: 133 S-NEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMNGHGCCTMLG 192
           S + GV+P++FV+ L+  F   +  I ++EN  +S+ WKD+G +V S  +   GC TM+G
Sbjct: 135 SAHGGVSPAEFVNALINGFGKTSLRIDADENTQVSMKWKDLGFTVCSTVLVSCGCTTMMG 194

Query: 193 PMSTQLKQRKTNI-SRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGILRRNKTVKL 252
           PM +++KQRK+ + +RKR++P    +P EV++  + +K+DTD NM++MF ILR+NK VK+
Sbjct: 195 PMYSEMKQRKSRVGNRKRTKPGAGVKPEEVDDTEAEKKSDTDNNMAVMFNILRKNKRVKI 254

Query: 253 EHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYS 312
           E+LVLNRKSFAQT EN+FALSFLVKDGR EIT+D NGSHFV P+NAPA + ++S EV Y+
Sbjct: 255 ENLVLNRKSFAQTAENMFALSFLVKDGRVEITVDNNGSHFVEPRNAPAANLVLSGEVAYN 314

Query: 313 HFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLPTTPIRKLS 372
           HFV RFD+KDW+ M  MV VGEELMP R         + + + D+ E           LS
Sbjct: 315 HFVLRFDYKDWEPMSKMVAVGEELMPLR---------ETKVAQDSIE-----------LS 368

Query: 373 RNRGLVMQ--SVVEDS 383
           R +G V+Q  +VVEDS
Sbjct: 375 RKQGSVIQEETVVEDS 368

BLAST of Tan0015367 vs. ExPASy Swiss-Prot
Match: Q6BDR8 (Non-structural maintenance of chromosome element 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nse4 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 4.6e-12
Identity = 69/288 (23.96%), Postives = 131/288 (45.49%), Query Frame = 0

Query: 58  STKRRVLRSRYLAVIHEISERREDL-SKDSDKFNVIINEVEKLHEQVQKPREQVADAEAL 117
           S  +R LR RY  +I+++ E R +L  ++++     I     L   V  P E   DA  L
Sbjct: 3   SIDKRDLRKRYRNLINKVQESRLELVDEENNNLYETITTANDLFSSVDAPTEATLDALLL 62

Query: 118 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEEN----NLISLSWKDIGL 177
               +  + SI+++      P   +    +         TS  N      I  +W  +G 
Sbjct: 63  TKTVD--LASIKARQLHIGRPKFNIELFTKNIKQFLNYPTSHSNVTRIQEIDTAWSRLGK 122

Query: 178 SVSSIFMNGHGCCTMLGPMSTQLKQRKTNISRK---RSRPTESSRPAEVNE-NGSVEKTD 237
             S+          M+GP+S + K+R  NI R+   +  P   ++P  +NE N + ++ +
Sbjct: 123 LASNCEKQPASLNLMVGPLSFRKKER--NIQRRERLQKAPNVLTQPTMLNERNITTQENN 182

Query: 238 TDKNMSIMFGILRRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHF 297
           T KN+  +  +L+ ++ V     + N +S+ QTVENLF +SFL K+G+A +  +++G   
Sbjct: 183 TTKNVLHISRLLQAHQPVNFLKFITNPQSYPQTVENLFYVSFLFKEGKAALVENESGILM 242

Query: 298 VSPKNAPAHSAIMSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHR 337
           +  +  P    +++ E+     V       ++ ++    + E ++P R
Sbjct: 243 LETRIPPTDDQVVAGEIRNIQLVLDMTMDLYENIIKEYNIKESIIPTR 286

BLAST of Tan0015367 vs. ExPASy Swiss-Prot
Match: Q9NXX6 (Non-structural maintenance of chromosomes element 4 homolog A OS=Homo sapiens OX=9606 GN=NSMCE4A PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 8.6e-11
Identity = 79/353 (22.38%), Postives = 148/353 (41.93%), Query Frame = 0

Query: 2   RPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGEDNATEEEEGQQDSTKR 61
           R   R  + S  S +S+ G A +     E    +     D+  E       E + D    
Sbjct: 23  RTRSRSRSRSPLSPRSRRGSARERREAPERPSLEDTEPSDSGDEMMDPASLEAEADQGLC 82

Query: 62  RVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIAN 121
           R +R +Y A+I+ + + RED+    DK   ++ E   L  +V + RE V DA  L  +A+
Sbjct: 83  RQIRHQYRALINSVQQNREDILNAGDKLTEVLEEANTLFNEVSRAREAVLDAHFLV-LAS 142

Query: 122 SLVTSIRSQSNEGVTPSD---FVSCLLREFA----DINGGITSEENN----LISLSWKDI 181
            L      Q    ++  D   +V  LL        +    I  E++     ++  SWK  
Sbjct: 143 DLGKEKAKQLRSDLSSFDMLRYVETLLTHMGVNPLEAEELIRDEDSPDFEFIVYDSWKIT 202

Query: 182 GLSVSSIFMNGHGCCTMLGPMSTQLKQRKTNISRKRSRPT---ESSRPAEVNENGSVEKT 241
           G +  + F   H    +LG +  +    K  + R R  P    E + PA++       + 
Sbjct: 203 GRTAENTFNKTHTFHFLLGSIYGECPVPKPRVDRPRKVPVIQEERAMPAQLRRMEESHQE 262

Query: 242 DTDKNMSIMFGILRR------NKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITI 301
            T+K +  + G+L+       +  +     V++  SF +TVEN+F +SF+++DG A I +
Sbjct: 263 ATEKEVERILGLLQTYFREDPDTPMSFFDFVVDPHSFPRTVENIFHVSFIIRDGFARIRL 322

Query: 302 DKNGSHFVSPKNAPAHSAIMSHEVNY-SHFVFRFDFKDWKLMMDMVPVGEELM 334
           D++    + P +    +    H     +  +    ++DW+ ++    + E ++
Sbjct: 323 DQDRLPVIEPVSINEENEGFEHNTQVRNQGIIALSYRDWEEIVKTFEISEPVI 374

BLAST of Tan0015367 vs. ExPASy Swiss-Prot
Match: Q2TBI1 (Non-structural maintenance of chromosomes element 4 homolog A OS=Bos taurus OX=9913 GN=NSMCE4A PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.5e-10
Identity = 85/354 (24.01%), Postives = 149/354 (42.09%), Query Frame = 0

Query: 2   RPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGE--DNATEEEEGQQDST 61
           R   R  + S  S  S+ G A +     E    +     D+  E  D A+ EEE   D +
Sbjct: 23  RTRSRSRSRSPLSPGSRRGAAPERREAPERPGLEDTEPSDSGDEMIDPASLEEE--TDPS 82

Query: 62  KRRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEAL--- 121
             R +R +Y A+I+ + + RED+   SDK   ++ E   L   V + RE V DA  L   
Sbjct: 83  LCRQIRHQYRALINSVQQNREDILNASDKLTEVLEEANTLFNGVSRAREAVLDAHFLVLA 142

Query: 122 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFAD--------INGGITSEENNLISLSWK 181
            D+       +RS  N       +V  LL             I    +S+   ++  SWK
Sbjct: 143 SDLGKEKAKQLRSDLN-SFDMLRYVETLLTHMGVNPLEAEELIRDEDSSDLEFIVYDSWK 202

Query: 182 DIGLSVSSIFMNGHGCCTMLGPMSTQLKQRKTNISRKRS---RPTESSRPAEVNENGSVE 241
             G +  + F   H    +LG +  +    K  I R R       + + PA++       
Sbjct: 203 ISGKTAENTFNKTHTFHFLLGSIQGECPVPKPRIERPRKVRMIEEQQAMPAQLKRMEESH 262

Query: 242 KTDTDKNMSIMFGILRR------NKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEI 301
           +  T+K +  + G+L+       +  +     V++  SF +TVEN+F +SF+++DG A I
Sbjct: 263 QEATEKEVERILGLLQTYFQEDPDTPMSFFDFVVDPHSFPRTVENIFHVSFIIRDGFARI 322

Query: 302 TIDKNGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELM 334
            +D++    + P N    S   +   N +  +    ++DW+ ++    + E ++
Sbjct: 323 RLDRDRLPVIEPVNINEESGGNTQIRNQA--IIALSYRDWEEIVRTFEISEPVI 371

BLAST of Tan0015367 vs. NCBI nr
Match: XP_022933648.1 (non-structural maintenance of chromosomes element 4 homolog A-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 699.9 bits (1805), Expect = 1.3e-197
Identity = 364/404 (90.10%), Postives = 385/404 (95.30%), Query Frame = 0

Query: 1   MRPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGEDNATEEEEGQQDSTK 60
           MRPVKREPAASSRSNQSK GDAVDAA+LR+VKR +VARE++N GE N  E EEGQQ  TK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDL+KDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMN 180
           NSLVTSI+SQSNEGVTPSDFVSCLL+EFADI GGITSEENN ISLSWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENNPISLSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNI+RKRSRPTESSRPAEV+E+GS  KTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEAKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTV+LEHL+LNR SFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLP 360
           MSHEVNYSHFVFRFDFKDWKLMMD+VPVGEELMPHR FLNSA MPQ EA+ D+SE+A+LP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQSVVEDSPDNGAHSGPTAILRCKRKLSQG 405
           TTPIRKLSRNRGLVMQ+VVEDSPDNGAHSGPTA+LRCK+KLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNGAHSGPTAMLRCKKKLSQG 404

BLAST of Tan0015367 vs. NCBI nr
Match: XP_022933647.1 (non-structural maintenance of chromosomes element 4 homolog A-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 699.9 bits (1805), Expect = 1.3e-197
Identity = 364/404 (90.10%), Postives = 385/404 (95.30%), Query Frame = 0

Query: 1   MRPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGEDNATEEEEGQQDSTK 60
           MRPVKREPAASSRSNQSK GDAVDAA+LR+VKR +VARE++N GE N  E EEGQQ  TK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDL+KDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMN 180
           NSLVTSI+SQSNEGVTPSDFVSCLL+EFADI GGITSEENN ISLSWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENNPISLSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNI+RKRSRPTESSRPAEV+E+GS  KTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEAKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTV+LEHL+LNR SFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLP 360
           MSHEVNYSHFVFRFDFKDWKLMMD+VPVGEELMPHR FLNSA MPQ EA+ D+SE+A+LP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQSVVEDSPDNGAHSGPTAILRCKRKLSQG 405
           TTPIRKLSRNRGLVMQ+VVEDSPDNGAHSGPTA+LRCK+KLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNGAHSGPTAMLRCKKKLSQG 404

BLAST of Tan0015367 vs. NCBI nr
Match: KAG7022067.1 (Non-structural maintenance of chromosomes element 4-like A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 698.0 bits (1800), Expect = 4.9e-197
Identity = 361/404 (89.36%), Postives = 386/404 (95.54%), Query Frame = 0

Query: 1   MRPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGEDNATEEEEGQQDSTK 60
           M+PVKREPAASSRSNQSK GDAVDAA+LR+VKR +VARE++N GE N  E EEGQQ  TK
Sbjct: 1   MKPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDL+KDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMN 180
           NSLVTSI+SQSNEGVTPSDFVSCLL+EFA+I GGITSEEN+ IS+SWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGIL 240
           GHGCCTMLGPMSTQLKQRKTNI+RKRSRPTESSRPAEV+E+GS EKTDTDKNMSIMFGIL
Sbjct: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTV+LEHL+LNR SFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLP 360
           MSHEVNYSHFVFRFDFKDWKLMMD+VPVGEELMPHR FLNSA MPQ EA+ D+SE+A+LP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQSVVEDSPDNGAHSGPTAILRCKRKLSQG 405
           TTPIRKLSRNRGLVMQ+VVEDSPDN AHSGPTA+LRCK+KLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404

BLAST of Tan0015367 vs. NCBI nr
Match: KAG6588168.1 (Non-structural maintenance of chromosomes element 4-like A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 696.4 bits (1796), Expect = 1.4e-196
Identity = 360/404 (89.11%), Postives = 386/404 (95.54%), Query Frame = 0

Query: 1   MRPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGEDNATEEEEGQQDSTK 60
           M+PVKREPAASSRSN+SK GDAVDAA+LR+VKR +VARE++N GE N  E EEGQQ  TK
Sbjct: 1   MKPVKREPAASSRSNRSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDL+KDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMN 180
           NSLVTSI+SQSNEGVTPSDFVSCLL+EFA+I GGITSEEN+ IS+SWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFANIEGGITSEENDPISVSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGIL 240
           GHGCCTMLGPMSTQLKQRKTNI+RKRSRPTESSRPAEV+E+GS EKTDTDKNMSIMFGIL
Sbjct: 181 GHGCCTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTV+LEHL+LNR SFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLP 360
           MSHEVNYSHFVFRFDFKDWKLMMD+VPVGEELMPHR FLNSA MPQ EA+ D+SE+A+LP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQSVVEDSPDNGAHSGPTAILRCKRKLSQG 405
           TTPIRKLSRNRGLVMQ+VVEDSPDN AHSGPTA+LRCK+KLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNDAHSGPTAMLRCKKKLSQG 404

BLAST of Tan0015367 vs. NCBI nr
Match: XP_022967561.1 (non-structural maintenance of chromosomes element 4 homolog A [Cucurbita maxima])

HSP 1 Score: 695.3 bits (1793), Expect = 3.2e-196
Identity = 363/405 (89.63%), Postives = 386/405 (95.31%), Query Frame = 0

Query: 1   MRPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGEDNATEEEEGQQDSTK 60
           MRPVKREPAASSRSNQSK GDAVDAA+LR+VKR +VARE++N G+ N  E EEGQQ  TK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGDGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDL+KDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMN 180
           NSLVTSI+SQSNEGVTPSDFVSCLL+EFADI GGITSEEN+ IS+SWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENDPISISWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNI+RKRSRPTESSRPAEV+E+GS EKTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTV+LEHL+LNR SFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLP 360
           MSHEVNYSHFVFRFDFKDWKLMMD+VPVGEELMPHR FLN+A MPQAEA+ D+SE+A LP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNTAPMPQAEAATDDSESAALP 360

Query: 361 TTPIRKLSRNRGLVMQSVVEDSPDN-GAHSGPTAILRCKRKLSQG 405
           TTPIRKLSRNRGLVMQSVVEDSPDN GAHSGPTA+LRCK+KLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQSVVEDSPDNDGAHSGPTAMLRCKKKLSQG 405

BLAST of Tan0015367 vs. ExPASy TrEMBL
Match: A0A6J1F5E6 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=3662 GN=LOC111441004 PE=3 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 6.3e-198
Identity = 364/404 (90.10%), Postives = 385/404 (95.30%), Query Frame = 0

Query: 1   MRPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGEDNATEEEEGQQDSTK 60
           MRPVKREPAASSRSNQSK GDAVDAA+LR+VKR +VARE++N GE N  E EEGQQ  TK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDL+KDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMN 180
           NSLVTSI+SQSNEGVTPSDFVSCLL+EFADI GGITSEENN ISLSWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENNPISLSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNI+RKRSRPTESSRPAEV+E+GS  KTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEAKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTV+LEHL+LNR SFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLP 360
           MSHEVNYSHFVFRFDFKDWKLMMD+VPVGEELMPHR FLNSA MPQ EA+ D+SE+A+LP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQSVVEDSPDNGAHSGPTAILRCKRKLSQG 405
           TTPIRKLSRNRGLVMQ+VVEDSPDNGAHSGPTA+LRCK+KLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNGAHSGPTAMLRCKKKLSQG 404

BLAST of Tan0015367 vs. ExPASy TrEMBL
Match: A0A6J1F0F0 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=3662 GN=LOC111441004 PE=3 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 6.3e-198
Identity = 364/404 (90.10%), Postives = 385/404 (95.30%), Query Frame = 0

Query: 1   MRPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGEDNATEEEEGQQDSTK 60
           MRPVKREPAASSRSNQSK GDAVDAA+LR+VKR +VARE++N GE N  E EEGQQ  TK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGEGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDL+KDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMN 180
           NSLVTSI+SQSNEGVTPSDFVSCLL+EFADI GGITSEENN ISLSWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENNPISLSWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNI+RKRSRPTESSRPAEV+E+GS  KTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEAKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTV+LEHL+LNR SFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLP 360
           MSHEVNYSHFVFRFDFKDWKLMMD+VPVGEELMPHR FLNSA MPQ EA+ D+SE+A+LP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNSAPMPQEEATTDDSESASLP 360

Query: 361 TTPIRKLSRNRGLVMQSVVEDSPDNGAHSGPTAILRCKRKLSQG 405
           TTPIRKLSRNRGLVMQ+VVEDSPDNGAHSGPTA+LRCK+KLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQTVVEDSPDNGAHSGPTAMLRCKKKLSQG 404

BLAST of Tan0015367 vs. ExPASy TrEMBL
Match: A0A6J1HSJ2 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita maxima OX=3661 GN=LOC111467024 PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.5e-196
Identity = 363/405 (89.63%), Postives = 386/405 (95.31%), Query Frame = 0

Query: 1   MRPVKREPAASSRSNQSKAGDAVDAASLREVKREKVARERDNSGEDNATEEEEGQQDSTK 60
           MRPVKREPAASSRSNQSK GDAVDAA+LR+VKR +VARE++N G+ N  E EEGQQ  TK
Sbjct: 1   MRPVKREPAASSRSNQSKGGDAVDAANLRDVKRARVAREKENIGDGNMAEAEEGQQGLTK 60

Query: 61  RRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120
           RRVLRSRYLAVIHEISERREDL+KDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA
Sbjct: 61  RRVLRSRYLAVIHEISERREDLTKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 120

Query: 121 NSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMN 180
           NSLVTSI+SQSNEGVTPSDFVSCLL+EFADI GGITSEEN+ IS+SWKDIGLSVSSIFMN
Sbjct: 121 NSLVTSIKSQSNEGVTPSDFVSCLLKEFADIEGGITSEENDPISISWKDIGLSVSSIFMN 180

Query: 181 GHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGIL 240
           GHGC TMLGPMSTQLKQRKTNI+RKRSRPTESSRPAEV+E+GS EKTDTDKNMSIMFGIL
Sbjct: 181 GHGCRTMLGPMSTQLKQRKTNIARKRSRPTESSRPAEVDEDGSEEKTDTDKNMSIMFGIL 240

Query: 241 RRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300
           RRNKTV+LEHL+LNR SFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI
Sbjct: 241 RRNKTVRLEHLILNRNSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAI 300

Query: 301 MSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLP 360
           MSHEVNYSHFVFRFDFKDWKLMMD+VPVGEELMPHR FLN+A MPQAEA+ D+SE+A LP
Sbjct: 301 MSHEVNYSHFVFRFDFKDWKLMMDIVPVGEELMPHRNFLNTAPMPQAEAATDDSESAALP 360

Query: 361 TTPIRKLSRNRGLVMQSVVEDSPDN-GAHSGPTAILRCKRKLSQG 405
           TTPIRKLSRNRGLVMQSVVEDSPDN GAHSGPTA+LRCK+KLSQG
Sbjct: 361 TTPIRKLSRNRGLVMQSVVEDSPDNDGAHSGPTAMLRCKKKLSQG 405

BLAST of Tan0015367 vs. ExPASy TrEMBL
Match: A0A6J1HBJ2 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=3662 GN=LOC111461359 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.7e-190
Identity = 360/410 (87.80%), Postives = 381/410 (92.93%), Query Frame = 0

Query: 1   MRPVKREPAASSRSNQSKAGD-AVDAASLREVKREK-VARERDNSGEDNATEEEEGQQDS 60
           MRPVKREPAASSRSN+S+ G  A DAASLR+VKREK V+RER+ SG DN TEEEEGQQ+ 
Sbjct: 1   MRPVKREPAASSRSNRSRGGAVAFDAASLRDVKREKVVSREREKSGVDNTTEEEEGQQEL 60

Query: 61  TKRRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQD 120
           +KRRVLRSRYLAVIHEISERREDLSKD DKFNVIINEVEKLHEQVQKPREQVADAEALQD
Sbjct: 61  SKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEALQD 120

Query: 121 IANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIF 180
           IA+SLVTSIRSQSNEGVTPS+FVSCLLREFADING ITSEEN+L+S+SWKDIGL VSSIF
Sbjct: 121 IASSLVTSIRSQSNEGVTPSNFVSCLLREFADINGNITSEENDLVSVSWKDIGLGVSSIF 180

Query: 181 MNGHGCCTMLGPMSTQLKQRK-TNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMF 240
           MNGHGC TMLGPMS QLKQRK TNISRKRSRPTES RPAEV +NGS EKTDTDKNM IMF
Sbjct: 181 MNGHGCRTMLGPMSNQLKQRKTTNISRKRSRPTESYRPAEVGDNGSEEKTDTDKNMGIMF 240

Query: 241 GILRRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAH 300
           GILRRNKTVKLEHL+LNRKSFAQTVENLFALSFLVKDGRA ITIDKNGSHFVSPKNAPAH
Sbjct: 241 GILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAAITIDKNGSHFVSPKNAPAH 300

Query: 301 SAIMSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAA 360
           SAIMSHEVNYSHFVFRFDFKDWK M+DMVPVG+ELMPHR  LNSA +PQAEAS D+SEAA
Sbjct: 301 SAIMSHEVNYSHFVFRFDFKDWKPMVDMVPVGDELMPHRNILNSAQVPQAEASTDDSEAA 360

Query: 361 TLPTTPIRKLSRNRGLVMQSVVEDSPDNGAHSGPTAILRCKRKLSQGLEN 408
            LPTTPIRKLSRNRGLVMQSVVEDSPDNGAHS  T+ILRCKRKL+QG+ N
Sbjct: 361 ALPTTPIRKLSRNRGLVMQSVVEDSPDNGAHSRATSILRCKRKLNQGMVN 410

BLAST of Tan0015367 vs. ExPASy TrEMBL
Match: A0A6J1JLE2 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita maxima OX=3661 GN=LOC111485470 PE=3 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 2.4e-189
Identity = 356/409 (87.04%), Postives = 376/409 (91.93%), Query Frame = 0

Query: 1   MRPVKREPAASSRSNQSKAGD-AVDAASLREVKREKVARERDNSGEDNATEEEEGQQDST 60
           MRPVKREPA+SSR+N+S+ G  AVDAASLR+VKRE+VA ER+ SG DN T EEEGQQ+ +
Sbjct: 1   MRPVKREPASSSRNNRSRGGSVAVDAASLRDVKRERVAWEREKSGVDNTTVEEEGQQELS 60

Query: 61  KRRVLRSRYLAVIHEISERREDLSKDSDKFNVIINEVEKLHEQVQKPREQVADAEALQDI 120
           KRRVLRSRYLAVIHEISERREDLSKD DKFNVIINEVEKLHEQV KPREQVADAEALQDI
Sbjct: 61  KRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVHKPREQVADAEALQDI 120

Query: 121 ANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFM 180
           A+SLVTSI SQSNEGVTPSDFVSCLLREFADING IT EENNL+S+SWKDIGL VSSIFM
Sbjct: 121 ASSLVTSIMSQSNEGVTPSDFVSCLLREFADINGNITCEENNLVSVSWKDIGLGVSSIFM 180

Query: 181 NGHGCCTMLGPMSTQLKQRK-TNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFG 240
           N HGC TMLGPMS QLKQRK TNISRKRSRPTES RPAEV +NGS EKTDTDKNM IMFG
Sbjct: 181 NCHGCRTMLGPMSNQLKQRKTTNISRKRSRPTESYRPAEVEDNGSEEKTDTDKNMGIMFG 240

Query: 241 ILRRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHS 300
           ILRRNKTVKLEHL+LNRKSFAQTVENLFALSFLVKDGRA ITIDKNGSHFVSPKNAPAHS
Sbjct: 241 ILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAAITIDKNGSHFVSPKNAPAHS 300

Query: 301 AIMSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAAT 360
           AIMSHEVNYSHF+FRFDF DWK M+DMVPVG+ELMPHR  LNSA +PQAEAS D+SEAA 
Sbjct: 301 AIMSHEVNYSHFIFRFDFMDWKPMVDMVPVGDELMPHRNILNSAQVPQAEASTDDSEAAA 360

Query: 361 LPTTPIRKLSRNRGLVMQSVVEDSPDNGAHSGPTAILRCKRKLSQGLEN 408
           LPTTPIRKLSRNRGLVMQSVVEDSPDNGAHSG TAILRCKRKLSQG+ N
Sbjct: 361 LPTTPIRKLSRNRGLVMQSVVEDSPDNGAHSGATAILRCKRKLSQGMVN 409

BLAST of Tan0015367 vs. TAIR 10
Match: AT1G51130.1 (Nse4, component of Smc5/6 DNA repair complex )

HSP 1 Score: 381.7 bits (979), Expect = 7.3e-106
Identity = 222/406 (54.68%), Postives = 290/406 (71.43%), Query Frame = 0

Query: 2   RPVKREPAASSRSNQSKAGDAVDAASLREVKREKVAR-ERDNSGEDNATEEEEGQQDSTK 61
           + VKRE  A+    + +A D  +   LR VK+EK  + E D+   D     +E +Q  + 
Sbjct: 3   KTVKRESEAT--GGKREADD--EPEKLRSVKKEKQRKTEADSVRPDEPPPPQEEEQGISD 62

Query: 62  RRVLRSRYLAVIHEISERREDLSK-DSDKFNVIINEVEKLHEQVQKPREQVADAEALQDI 121
           RR+LRS+YL++ +EI++ ++DL K DSDKF+ IIN VE LH+QV+KPREQ+ADAEAL DI
Sbjct: 63  RRILRSKYLSLQNEINDCKDDLMKIDSDKFSRIINAVENLHQQVRKPREQIADAEALLDI 122

Query: 122 ANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFM 181
           AN+L++S++SQS  GV+P++FV+ L+  F   + GI ++E   +SL WKD+G +V S  +
Sbjct: 123 ANTLMSSVKSQSAHGVSPAEFVNALISGFGQGSLGIDTDETAQVSLKWKDLGFAVCSTVL 182

Query: 182 NGHGCCTMLGPMSTQLKQRKTNISRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGI 241
              GC TMLGPM T+LKQRK   +RKR++P E  RP EV+++ S EKTDTDKNM+IMF I
Sbjct: 183 VSCGCSTMLGPMDTELKQRKRAPNRKRTKPGEGVRPDEVDDSQSEEKTDTDKNMTIMFNI 242

Query: 242 LRRNKTVKLEHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSA 301
           L + K V+LE+LVLNR+SFAQTVENLFALSFL KDGR EI +DK+GSHF  P+NAP  + 
Sbjct: 243 LGKKKRVQLENLVLNRRSFAQTVENLFALSFLAKDGRVEIIVDKSGSHFAMPRNAPDANV 302

Query: 302 IMSHEVNYSHFVFRFDFKDWKLMMDMVPVGEELMPHR-IFLNSASMPQAEASADNSEAAT 361
           +MS EV Y+HFVFR DFKDWKLM +MVP+GEELMPHR   + S+S P A A A       
Sbjct: 303 VMSGEVIYNHFVFRLDFKDWKLMSEMVPLGEELMPHRQTAVASSSCPAASAPASADFTQD 362

Query: 362 LPTTPIRKLSRNRGLVMQ--SVVEDSPDNGAHSGPTAILRCKRKLS 403
             TTPIRKLSRNRGLV+Q  +VVED+PD     G     RCKR+L+
Sbjct: 363 TQTTPIRKLSRNRGLVVQEETVVEDTPDK---EGDGTRRRCKRRLT 401

BLAST of Tan0015367 vs. TAIR 10
Match: AT3G20760.1 (Nse4, component of Smc5/6 DNA repair complex )

HSP 1 Score: 317.0 bits (811), Expect = 2.2e-86
Identity = 183/376 (48.67%), Postives = 260/376 (69.15%), Query Frame = 0

Query: 13  RSNQSKAGDAVDAASL-REVKREKVARERDNSGEDNATEEEEGQQDSTKRRVLRSRYLAV 72
           RS + +A ++ +   + ++ KR K         +    EEEE  Q    RR +RS+YLA+
Sbjct: 15  RSRRREADESSETPKIVKKEKRSKTVIASLRPQQPPIQEEEE--QGIADRRAIRSQYLAL 74

Query: 73  IHEISERREDLSK-DSDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQ 132
            H+I + ++DL+K DS+KFN IINEVE LH++V+KPREQ+ADAEAL D+ NS+V+S++SQ
Sbjct: 75  THKIKDAKDDLTKIDSNKFNSIINEVENLHQKVRKPREQIADAEALLDLTNSVVSSVKSQ 134

Query: 133 S-NEGVTPSDFVSCLLREFADINGGITSEENNLISLSWKDIGLSVSSIFMNGHGCCTMLG 192
           S + GV+P++FV+ L+  F   +  I ++EN  +S+ WKD+G +V S  +   GC TM+G
Sbjct: 135 SAHGGVSPAEFVNALINGFGKTSLRIDADENTQVSMKWKDLGFTVCSTVLVSCGCTTMMG 194

Query: 193 PMSTQLKQRKTNI-SRKRSRPTESSRPAEVNENGSVEKTDTDKNMSIMFGILRRNKTVKL 252
           PM +++KQRK+ + +RKR++P    +P EV++  + +K+DTD NM++MF ILR+NK VK+
Sbjct: 195 PMYSEMKQRKSRVGNRKRTKPGAGVKPEEVDDTEAEKKSDTDNNMAVMFNILRKNKRVKI 254

Query: 253 EHLVLNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAPAHSAIMSHEVNYS 312
           E+LVLNRKSFAQT EN+FALSFLVKDGR EIT+D NGSHFV P+NAPA + ++S EV Y+
Sbjct: 255 ENLVLNRKSFAQTAENMFALSFLVKDGRVEITVDNNGSHFVEPRNAPAANLVLSGEVAYN 314

Query: 313 HFVFRFDFKDWKLMMDMVPVGEELMPHRIFLNSASMPQAEASADNSEAATLPTTPIRKLS 372
           HFV RFD+KDW+ M  MV VGEELMP R         + + + D+ E           LS
Sbjct: 315 HFVLRFDYKDWEPMSKMVAVGEELMPLR---------ETKVAQDSIE-----------LS 368

Query: 373 RNRGLVMQ--SVVEDS 383
           R +G V+Q  +VVEDS
Sbjct: 375 RKQGSVIQEETVVEDS 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6891.0e-10454.68Non-structural maintenance of chromosomes element 4 homolog A OS=Arabidopsis tha... [more]
F4JET13.1e-8548.67Non-structural maintenance of chromosomes element 4 homolog B OS=Arabidopsis tha... [more]
Q6BDR84.6e-1223.96Non-structural maintenance of chromosome element 4 OS=Schizosaccharomyces pombe ... [more]
Q9NXX68.6e-1122.38Non-structural maintenance of chromosomes element 4 homolog A OS=Homo sapiens OX... [more]
Q2TBI11.5e-1024.01Non-structural maintenance of chromosomes element 4 homolog A OS=Bos taurus OX=9... [more]
Match NameE-valueIdentityDescription
XP_022933648.11.3e-19790.10non-structural maintenance of chromosomes element 4 homolog A-like isoform X2 [C... [more]
XP_022933647.11.3e-19790.10non-structural maintenance of chromosomes element 4 homolog A-like isoform X1 [C... [more]
KAG7022067.14.9e-19789.36Non-structural maintenance of chromosomes element 4-like A [Cucurbita argyrosper... [more]
KAG6588168.11.4e-19689.11Non-structural maintenance of chromosomes element 4-like A, partial [Cucurbita a... [more]
XP_022967561.13.2e-19689.63non-structural maintenance of chromosomes element 4 homolog A [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1F5E66.3e-19890.10Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=366... [more]
A0A6J1F0F06.3e-19890.10Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=366... [more]
A0A6J1HSJ21.5e-19689.63Non-structural maintenance of chromosomes element 4 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1HBJ21.7e-19087.80Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=366... [more]
A0A6J1JLE22.4e-18987.04Non-structural maintenance of chromosomes element 4 OS=Cucurbita maxima OX=3661 ... [more]
Match NameE-valueIdentityDescription
AT1G51130.17.3e-10654.68Nse4, component of Smc5/6 DNA repair complex [more]
AT3G20760.12.2e-8648.67Nse4, component of Smc5/6 DNA repair complex [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014854Non-structural maintenance of chromosome element 4, C-terminalPFAMPF08743Nse4_Ccoord: 246..335
e-value: 1.2E-25
score: 89.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 195..227
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..45
NoneNo IPR availablePANTHERPTHR16140:SF18NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4coord: 30..401
IPR027786Nse4/EID familyPANTHERPTHR16140UNCHARACTERIZEDcoord: 30..401

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0015367.1Tan0015367.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006310 DNA recombination
biological_process GO:0006281 DNA repair
cellular_component GO:0005634 nucleus
cellular_component GO:0030915 Smc5-Smc6 complex