Tan0014893 (gene) Snake gourd v1

BLAST of Tan0014893 vs. ExPASy Swiss-Prot
Match: Q9LFY0 (Protein PTST homolog 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST2 PE=1 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 4.1e-98
Identity = 216/472 (45.76%), Postives = 291/472 (61.65%), Query Frame = 0

Query: 63  CSGFLRRCK----GLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVK 122
           C+G  R C     G DSEG   LE EILEFM++S  PG FPSKKDLI +GR DLV+ IV 
Sbjct: 72  CNGVSRMCNKRNLGWDSEGSKDLETEILEFMKNSEKPGMFPSKKDLIRSGRFDLVERIVN 131

Query: 123 KGGWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFATAASSST 182
           +GGWL +GW+LDE+E+       +   E++   D     +  +  S E  +         
Sbjct: 132 QGGWLSMGWDLDEQEE------KVRVNENVTPQDLHIEKQLPNCNSPEMDKTLNHGDLDL 191

Query: 183 SASFTGRSLETVAEHESGIEGMLSRLEKERNAAFGACKNDLHPETTAKETVADQ----QR 242
           S++ +  + +  + ++SGIEG+L+RLEKERN + G    +      A   ++        
Sbjct: 192 SSNLSSSTEQVESRNDSGIEGILTRLEKERNLSLGISVRENGKSNGAMHDISPNGSVPWS 251

Query: 243 STISTPLSAKEGKWNDLEGYLSQTSSPGPVQIN---GLGGSVSSEMQRTWSTFKTDLQDG 302
           S I T    +E   +   G  +Q+   G   ++   GL  S +SE  RTWS  +    D 
Sbjct: 252 SRIVTASEIQEVDGSRGSGEYAQSRYQGAKSVSGKPGLSDSPTSETWRTWSMRRAGFTDE 311

Query: 303 DFKGSEIALSEGRSKEVLDVLRYETLGSRGDLTNSN----SGEEEMDHTQIQRHLQNLQL 362
           DF+ +EI+     S  +  V + +T    GD  N      S  E+++ T I+  LQ LQ 
Sbjct: 312 DFEAAEIS-----SSGLTGVKKDDTKKDSGDSMNGKDRIASSSEDVNKTHIKHRLQQLQS 371

Query: 363 ELSSVIGSLRS--NAFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRARLAVV 422
           ELSSV+ SLRS  +   + K    ++ +L  LSD +E++ENEI++A++KLRS RA+LAV+
Sbjct: 372 ELSSVLHSLRSPPDKVVTSKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAKLAVL 431

Query: 423 EGKMALTIIDAQKVTEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRK 482
           EGKMA+ IIDAQ++  EKQ+RI+ A RAL+ LRTA +VWPNSA+EVLL GSFDGWSTQRK
Sbjct: 432 EGKMAMAIIDAQRIVREKQRRIDHASRALRLLRTASIVWPNSASEVLLTGSFDGWSTQRK 491

Query: 483 MEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLIIT 518
           M+K+  GVFSL LKLYPGKYEIKFIVDGQWK+DPLRPIV S GYENNLLII+
Sbjct: 492 MKKAENGVFSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLIIS 532

BLAST of Tan0014893 vs. ExPASy Swiss-Prot
Match: Q10F03 (Protein FLOURY ENDOSPERM 6, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=FLO6 PE=1 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.5e-68
Identity = 175/466 (37.55%), Postives = 261/466 (56.01%), Query Frame = 0

Query: 75  SEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKGGWLCLGWNLDEEED 134
           + G+  LE  I EFM  S  PGAFP++ +L+ AGR DL  A+   GGWL LGW+    E 
Sbjct: 76  ARGEEDLEEAIYEFMRRSDKPGAFPTRAELVAAGRADLAAAVDACGGWLSLGWSSGGAEA 135

Query: 135 G------GIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFATAASSSTSASFTGRSLE 194
           G      G+   Y  +  +  AA            SD  Q    A+S    AS +GR  E
Sbjct: 136 GRASSSVGVHPDYPPEAGAAAAA---------GGASDLAQGAVWASSREAEASPSGRQPE 195

Query: 195 TVAEHE-----SGIEGMLSRLEKERNAAFGACKNDLHPETTAKETVADQQRSTISTPLSA 254
           T  E       +G++GML+RL++ER                 ++ VA   +S   +  SA
Sbjct: 196 TEEEETETKFGTGLDGMLTRLQRERERVRPPLPRSSDGAGGERDNVALMGQSGAPSH-SA 255

Query: 255 KEGKWN----DLEGYLSQTSSPGPVQINGLGGSVSSEMQRTWSTFKTDLQDGDFKGSEIA 314
             G++     D     S     G ++ N    S++++  RTWS  K      DF+ +EI 
Sbjct: 256 TGGRYTPKVPDNGNIHSYHPQNGALEHNKSSKSLTNDAWRTWSLDKGGF--SDFQAAEIH 315

Query: 315 LSEGRSK---EVLDVLRYETLGSRGD---LTNSNSGEEEMDHTQIQRHLQNLQLELSSVI 374
            +  R     + LD+L  + +    +   + + +  + + +   I   LQNL+L+L++ +
Sbjct: 316 STNSRKSFRHDGLDILAQDDVHGPSNGVAVHDYDINDVDSERDDIHARLQNLELDLTAAL 375

Query: 375 GSLRS--NAFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRARLAVVEGKMAL 434
            +LRS  +   S+  +   +     LSD +EF+E +++ A+++LRSIRA++AV+EGKMAL
Sbjct: 376 HTLRSRFDKVISDMSEGDGAKAPNGLSDDWEFEETKVMQAQEELRSIRAKIAVLEGKMAL 435

Query: 435 TIIDAQKVTEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMEKSST 494
            II+  K+ EEKQ+R++ A +AL  LRT  +VW N A+EVLL GSFDGW++QR+ME+S  
Sbjct: 436 EIIEKNKIIEEKQRRLDEAEKALSELRTVYIVWSNPASEVLLTGSFDGWTSQRRMERSER 495

Query: 495 GVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLIIT 518
           G FSL L+LYPG+YEIKFIVDG W+ DPLRP+V++NG+ENNLL +T
Sbjct: 496 GTFSLNLRLYPGRYEIKFIVDGVWRNDPLRPLVSNNGHENNLLTVT 529

BLAST of Tan0014893 vs. ExPASy Swiss-Prot
Match: F4KFB3 (Protein PTST homolog 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 7.6e-12
Identity = 44/137 (32.12%), Postives = 69/137 (50.36%), Query Frame = 0

Query: 384 QENEILNAKDKLRSIRARLAVVEGKMALTIIDAQKVTEEKQKRINCARRALQFLRTACVV 443
           +E E+   K+++   +  L+V++ +    I  AQ +  EK+  +  A  +L  L+   + 
Sbjct: 457 KELELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEESLSGLQEVVIE 516

Query: 444 WPNSAAEVLLVGSFDGWSTQRKMEKSST------GVFSLFLKLYPGKYEIKFIVDGQWKI 503
           +      V + GSF+GW  +  ME  ++        +S  L LYPG YEIKFIVDGQW  
Sbjct: 517 YCGDGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYEIKFIVDGQWIT 576

Query: 504 DPLRPIVNSNGYENNLL 515
           DP +  V      NN+L
Sbjct: 577 DPQKDSVTRGHISNNIL 593

BLAST of Tan0014893 vs. ExPASy Swiss-Prot
Match: Q94AX2 (Protein PTST, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 2.4e-10
Identity = 63/220 (28.64%), Postives = 103/220 (46.82%), Query Frame = 0

Query: 324 TNSNSGEEEMDHTQIQRHLQNLQLELSSVIGSLRSNAFASEKGQSKSSDDLLELSDAFEF 383
           T  +S  E++    + R L + +L+ S +I + RS      K  S+++     L    + 
Sbjct: 66  TEESSLSEDLLDESLSRPLTSDELK-SLLIDTERSKLV---KKLSEANQQNRFLKRQLKT 125

Query: 384 QENEILNAKDKLRSIR-------------ARLAVVEGKM----------ALTIIDA-QKV 443
           QE+EI N K +L  +              A L + +G             L+ +DA QK 
Sbjct: 126 QEHEITNIKTELALMELEVQALVKLAEEIANLGIPQGSRKISGKYIQSHLLSRLDAVQKK 185

Query: 444 TEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSLF-- 503
            +E+ K +  A+      +   V W   A  V ++GSFDGWS +  +    + +F+ F  
Sbjct: 186 MKEQIKGVEAAQS-----KEVHVFWIGMAESVQVMGSFDGWSQREDLSPEYSALFTKFST 245

Query: 504 -LKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLII 517
            L L PG+YE+KF+VDG+W+I P  P       ENN+L++
Sbjct: 246 TLFLRPGRYEMKFLVDGEWQISPEFPTSGEGLMENNVLVV 276

BLAST of Tan0014893 vs. ExPASy Swiss-Prot
Match: Q6PAM0 (5'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.9e-08
Identity = 34/92 (36.96%), Postives = 49/92 (53.26%), Query Frame = 0

Query: 424 QKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPG 483
           Q+ ++ + +  Q  R   + W     EV + GSF+ WST+  + KS    F   L L  G
Sbjct: 62  QQDLDDSVKPAQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHND-FVAILDLPEG 121

Query: 484 KYEIKFIVDGQWKIDPLRPIVNSN-GYENNLL 515
           +++ KF VDGQW  DP  P+V S  G  NNL+
Sbjct: 122 EHQYKFFVDGQWVHDPSEPVVTSQLGTINNLI 152

BLAST of Tan0014893 vs. NCBI nr
Match: XP_022968253.1 (protein PTST homolog 2, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 879.8 bits (2272), Expect = 1.2e-251
Identity = 455/521 (87.33%), Postives = 478/521 (91.75%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTAFGCLTPCVFPSIVFVSNERCAWNSHHMAATERRCSLLSSKGLF 60
           MLLFTN LPTSYFFPT FGCL+PC FPS VFVS  RC  NS+HMAATERRCSLLSSKG+F
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFVS--RCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSGF+RRCKGLDSEG+FALEAEILEFMESSRNP AFPSKKDLIEAGREDLVDAIVKKG
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFAT----AASS 180
           GWLCLGWNL++EEDGGI Q YLEDRESILA  WDSFGEFNSFKSDERQRFAT     ASS
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSASFTGRSLETVAEHESGIEGMLSRLEKERNAAFGACKNDLHPETTAKETVADQQRST 240
           STS+SFT RS ETVAEHESGIEGML+RLE+ERNA FG  KN LH ETTAKETVAD Q   
Sbjct: 181 STSSSFTSRSQETVAEHESGIEGMLNRLERERNATFGTGKNGLHSETTAKETVADLQEIP 240

Query: 241 ISTPLSAKEGKWNDLEGYLSQTSSPGPVQINGLGGSVSSEMQRTWSTFKTDLQDGDFKGS 300
           ISTPLSAKEGKW +L  YLS+ SS  PVQING GGSVSSE+QRTWSTFKTDLQDGDFKGS
Sbjct: 241 ISTPLSAKEGKWTNLGSYLSKNSS--PVQINGQGGSVSSEVQRTWSTFKTDLQDGDFKGS 300

Query: 301 EIALSEGRSKEVLDVLRYETLGSRGDLTNSNSGEEEMDHTQIQRHLQNLQLELSSVIGSL 360
           E+ALSE RS+EVLDVLRYETLGSRGD TNSNSGEEEM H QIQR++QNLQLELSS IGSL
Sbjct: 301 EMALSEVRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRYIQNLQLELSSAIGSL 360

Query: 361 RSNAFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRARLAVVEGKMALTIIDA 420
           RSNAFASEKGQSK SDDLLELSDAFEFQENEILNAKDKLRSIRA++AVVEGKMALTIIDA
Sbjct: 361 RSNAFASEKGQSKPSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDA 420

Query: 421 QKVTEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSL 480
           QKV EEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKME+SSTGVFSL
Sbjct: 421 QKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSL 480

Query: 481 FLKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLIIT 518
           FLKLYPGKYEIKFIVDGQWKIDPLRPIV+S+GYENNLLI+T
Sbjct: 481 FLKLYPGKYEIKFIVDGQWKIDPLRPIVSSDGYENNLLIVT 517

BLAST of Tan0014893 vs. NCBI nr
Match: KAG6574114.1 (Protein PTST-like 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 875.9 bits (2262), Expect = 1.7e-250
Identity = 453/521 (86.95%), Postives = 477/521 (91.55%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTAFGCLTPCVFPSIVFVSNERCAWNSHHMAATERRCSLLSSKGLF 60
           MLLFTN LPTSYFFP+ FGCL+P VFPSIVFVS  RC  NS+HMAATERRCSLLSSKG+F
Sbjct: 1   MLLFTNALPTSYFFPSPFGCLSPGVFPSIVFVS--RCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSGF+RRCK LD EG+FALEAEILEFMESSRNP AFPSKKDLIEAGREDLVDAIVKKG
Sbjct: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFAT----AASS 180
           GWLCLGWNL++EEDGGI Q YLEDRESILA  WDSFGEFNSFKSDERQRFAT     ASS
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSASFTGRSLETVAEHESGIEGMLSRLEKERNAAFGACKNDLHPETTAKETVADQQRST 240
           STS+SFT RS ETV EHESGIEGML+RLE+ERNA FG  KN LHPETT KETVAD Q   
Sbjct: 181 STSSSFTSRSQETVVEHESGIEGMLNRLERERNATFGTGKNCLHPETTVKETVADLQEIP 240

Query: 241 ISTPLSAKEGKWNDLEGYLSQTSSPGPVQINGLGGSVSSEMQRTWSTFKTDLQDGDFKGS 300
           ISTPLSAKEGKWN+L  YLS+ SS  PVQING GGSVSSE+Q TWSTFKTDL DGDFKGS
Sbjct: 241 ISTPLSAKEGKWNNLGSYLSKNSS--PVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGS 300

Query: 301 EIALSEGRSKEVLDVLRYETLGSRGDLTNSNSGEEEMDHTQIQRHLQNLQLELSSVIGSL 360
           E+ALSEGRS+EVLDVLRYETLGSRGD TNSNSGEEEM H QIQRH+QNLQLELSSVIGSL
Sbjct: 301 EMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELSSVIGSL 360

Query: 361 RSNAFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRARLAVVEGKMALTIIDA 420
           RSN+FASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRA++AVVEGKMALTIIDA
Sbjct: 361 RSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDA 420

Query: 421 QKVTEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSL 480
           QKV EEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKME+SSTGVFSL
Sbjct: 421 QKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSL 480

Query: 481 FLKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLIIT 518
           FLKLYPGKYEIKF+VDGQWKIDPLRPIV S+GYENNLLI+T
Sbjct: 481 FLKLYPGKYEIKFVVDGQWKIDPLRPIVRSDGYENNLLIVT 517

BLAST of Tan0014893 vs. NCBI nr
Match: XP_022944969.1 (protein PTST homolog 2, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 864.0 bits (2231), Expect = 6.6e-247
Identity = 452/521 (86.76%), Postives = 473/521 (90.79%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTAFGCLTPCVFPSIVFVSNERCAWNSHHMAATERRCSLLSSKGLF 60
           MLLFTNVLPTSYFFPT FGCL+P VFPSIVFVS  RC      MAATERRCSLLSSKG+F
Sbjct: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVS--RCG----GMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSGF+RRCK LD EG+FALEAEILEFMESSRNP AFPSKKDLIEAGREDLVDAIVKKG
Sbjct: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFAT----AASS 180
           GWLCLGWNL++EEDGGI Q YLEDRESILA  WDSFGEFNSFKSDERQRFAT     ASS
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSASFTGRSLETVAEHESGIEGMLSRLEKERNAAFGACKNDLHPETTAKETVADQQRST 240
           STS+SFT RS ETV EHESGIEGML+RLE+ERN  FG  KN LHPETT KETVAD Q   
Sbjct: 181 STSSSFTSRSQETVVEHESGIEGMLNRLERERNVTFGTGKNCLHPETTVKETVADLQEIP 240

Query: 241 ISTPLSAKEGKWNDLEGYLSQTSSPGPVQINGLGGSVSSEMQRTWSTFKTDLQDGDFKGS 300
           ISTPLSAKEGKWN+L  YLS+ SS  PVQING GGSVSSE+Q TWSTFKTDL DGDFKGS
Sbjct: 241 ISTPLSAKEGKWNNLGSYLSKNSS--PVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGS 300

Query: 301 EIALSEGRSKEVLDVLRYETLGSRGDLTNSNSGEEEMDHTQIQRHLQNLQLELSSVIGSL 360
           E+ALSEGRS+EVLDVLRYETLGSRGD TNSNSGEEEM H QIQRH+QNLQLELSSVIGSL
Sbjct: 301 EMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELSSVIGSL 360

Query: 361 RSNAFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRARLAVVEGKMALTIIDA 420
           RSN+FASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRA++AVVEGKMALTIIDA
Sbjct: 361 RSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDA 420

Query: 421 QKVTEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSL 480
           QKV EEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKME+SSTGVFSL
Sbjct: 421 QKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSL 480

Query: 481 FLKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLIIT 518
           FLKLYPGKYEIKFIVDGQWKIDPLRPIV S+GYENNLLI+T
Sbjct: 481 FLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 513

BLAST of Tan0014893 vs. NCBI nr
Match: XP_022968252.1 (protein PTST homolog 2, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 863.6 bits (2230), Expect = 8.6e-247
Identity = 455/552 (82.43%), Postives = 478/552 (86.59%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTAFGCLTPCVFPSIVFVSNERCAWNSHHMAATERRCSLLSSKGLF 60
           MLLFTN LPTSYFFPT FGCL+PC FPS VFVS  RC  NS+HMAATERRCSLLSSKG+F
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFVS--RCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSGF+RRCKGLDSEG+FALEAEILEFMESSRNP AFPSKKDLIEAGREDLVDAIVKKG
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFAT----AASS 180
           GWLCLGWNL++EEDGGI Q YLEDRESILA  WDSFGEFNSFKSDERQRFAT     ASS
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSASFTGRS-------------------------------LETVAEHESGIEGMLSRLE 240
           STS+SFT RS                                ETVAEHESGIEGML+RLE
Sbjct: 181 STSSSFTSRSQVFYSMPMKRNLIFYDNKMLCQDVKDTNFTNRETVAEHESGIEGMLNRLE 240

Query: 241 KERNAAFGACKNDLHPETTAKETVADQQRSTISTPLSAKEGKWNDLEGYLSQTSSPGPVQ 300
           +ERNA FG  KN LH ETTAKETVAD Q   ISTPLSAKEGKW +L  YLS+ SS  PVQ
Sbjct: 241 RERNATFGTGKNGLHSETTAKETVADLQEIPISTPLSAKEGKWTNLGSYLSKNSS--PVQ 300

Query: 301 INGLGGSVSSEMQRTWSTFKTDLQDGDFKGSEIALSEGRSKEVLDVLRYETLGSRGDLTN 360
           ING GGSVSSE+QRTWSTFKTDLQDGDFKGSE+ALSE RS+EVLDVLRYETLGSRGD TN
Sbjct: 301 INGQGGSVSSEVQRTWSTFKTDLQDGDFKGSEMALSEVRSREVLDVLRYETLGSRGDPTN 360

Query: 361 SNSGEEEMDHTQIQRHLQNLQLELSSVIGSLRSNAFASEKGQSKSSDDLLELSDAFEFQE 420
           SNSGEEEM H QIQR++QNLQLELSS IGSLRSNAFASEKGQSK SDDLLELSDAFEFQE
Sbjct: 361 SNSGEEEMSHNQIQRYIQNLQLELSSAIGSLRSNAFASEKGQSKPSDDLLELSDAFEFQE 420

Query: 421 NEILNAKDKLRSIRARLAVVEGKMALTIIDAQKVTEEKQKRINCARRALQFLRTACVVWP 480
           NEILNAKDKLRSIRA++AVVEGKMALTIIDAQKV EEKQKRINCARRALQFLRTACVVWP
Sbjct: 421 NEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWP 480

Query: 481 NSAAEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVN 518
           NSAAEVLLVGSFDGWSTQRKME+SSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV+
Sbjct: 481 NSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVS 540

BLAST of Tan0014893 vs. NCBI nr
Match: KAG7013174.1 (Protein PTST, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 861.3 bits (2224), Expect = 4.3e-246
Identity = 452/545 (82.94%), Postives = 477/545 (87.52%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTAFGCLTPCVFPSIVFVSNERCAWNSHHMAATERRCSLLSSKGLF 60
           MLLFTN LPTSYFFP+ FGCL+P VFPSIVFVS  RC  NS+HMAATERRCSLLSSKG+F
Sbjct: 1   MLLFTNALPTSYFFPSPFGCLSPGVFPSIVFVS--RCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSGF+RRCK LD EG+FALEAEILEFMESSRNP AFPSKKDLIEAGREDLVDAIVKKG
Sbjct: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFAT----AASS 180
           GWLCLGWNL++EEDGGI Q YLEDRESILA  WDSFGEFNSFKSDERQRFAT     ASS
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSASFTGRSLETVAEHESGIEGMLSRLEKERNAAFGACKNDLHPETTAKETVADQQRST 240
           STS+SFT RS ETV EHESGIEGML+RLE+ERNA FG  KN LHPETT KETVAD Q   
Sbjct: 181 STSSSFTSRSQETVVEHESGIEGMLNRLERERNATFGTGKNCLHPETTVKETVADLQEIP 240

Query: 241 ISTPLSAKEGKWNDLEGYLSQTSSPGPVQINGLGGSVSSEMQRTWSTFKTDLQDGDFKGS 300
           ISTPLSAKEGKWN+L  YLS+ SS  PVQING GGSVSSE+Q TWSTFKTDL DGDFKGS
Sbjct: 241 ISTPLSAKEGKWNNLGSYLSKNSS--PVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGS 300

Query: 301 EIALSEGRSKEVLDVLRYETLGSRGDLTNSNSGEEEMDHTQIQRHLQNLQLELSSVIGSL 360
           E+ALSEGRS+EVLDVLRYETLGSRGD TNSNSGEEEM H QIQRH+QNLQLELSSVIGSL
Sbjct: 301 EMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELSSVIGSL 360

Query: 361 RSNAFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRARLAVVEGKMALTII-- 420
           RSN+FASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRA++AVVEGKMALTI+  
Sbjct: 361 RSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIMYE 420

Query: 421 ----------------------DAQKVTEEKQKRINCARRALQFLRTACVVWPNSAAEVL 480
                                 DAQKV EEKQKRINCARRALQFLRTACVVWPNSAAEVL
Sbjct: 421 KLVLCVVICSIYGVSMTFCLYSDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVL 480

Query: 481 LVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENN 518
           LVGSFDGWSTQRKME+SSTGVFSLFLKLYPGKYEIKF+VDGQWKIDPLRPIV S+GYENN
Sbjct: 481 LVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFVVDGQWKIDPLRPIVRSDGYENN 540

BLAST of Tan0014893 vs. ExPASy TrEMBL
Match: A0A6J1HXH5 (protein PTST homolog 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467545 PE=4 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 5.6e-252
Identity = 455/521 (87.33%), Postives = 478/521 (91.75%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTAFGCLTPCVFPSIVFVSNERCAWNSHHMAATERRCSLLSSKGLF 60
           MLLFTN LPTSYFFPT FGCL+PC FPS VFVS  RC  NS+HMAATERRCSLLSSKG+F
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFVS--RCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSGF+RRCKGLDSEG+FALEAEILEFMESSRNP AFPSKKDLIEAGREDLVDAIVKKG
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFAT----AASS 180
           GWLCLGWNL++EEDGGI Q YLEDRESILA  WDSFGEFNSFKSDERQRFAT     ASS
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSASFTGRSLETVAEHESGIEGMLSRLEKERNAAFGACKNDLHPETTAKETVADQQRST 240
           STS+SFT RS ETVAEHESGIEGML+RLE+ERNA FG  KN LH ETTAKETVAD Q   
Sbjct: 181 STSSSFTSRSQETVAEHESGIEGMLNRLERERNATFGTGKNGLHSETTAKETVADLQEIP 240

Query: 241 ISTPLSAKEGKWNDLEGYLSQTSSPGPVQINGLGGSVSSEMQRTWSTFKTDLQDGDFKGS 300
           ISTPLSAKEGKW +L  YLS+ SS  PVQING GGSVSSE+QRTWSTFKTDLQDGDFKGS
Sbjct: 241 ISTPLSAKEGKWTNLGSYLSKNSS--PVQINGQGGSVSSEVQRTWSTFKTDLQDGDFKGS 300

Query: 301 EIALSEGRSKEVLDVLRYETLGSRGDLTNSNSGEEEMDHTQIQRHLQNLQLELSSVIGSL 360
           E+ALSE RS+EVLDVLRYETLGSRGD TNSNSGEEEM H QIQR++QNLQLELSS IGSL
Sbjct: 301 EMALSEVRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRYIQNLQLELSSAIGSL 360

Query: 361 RSNAFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRARLAVVEGKMALTIIDA 420
           RSNAFASEKGQSK SDDLLELSDAFEFQENEILNAKDKLRSIRA++AVVEGKMALTIIDA
Sbjct: 361 RSNAFASEKGQSKPSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDA 420

Query: 421 QKVTEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSL 480
           QKV EEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKME+SSTGVFSL
Sbjct: 421 QKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSL 480

Query: 481 FLKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLIIT 518
           FLKLYPGKYEIKFIVDGQWKIDPLRPIV+S+GYENNLLI+T
Sbjct: 481 FLKLYPGKYEIKFIVDGQWKIDPLRPIVSSDGYENNLLIVT 517

BLAST of Tan0014893 vs. ExPASy TrEMBL
Match: A0A6J1FZM9 (protein PTST homolog 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449347 PE=4 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.2e-247
Identity = 452/521 (86.76%), Postives = 473/521 (90.79%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTAFGCLTPCVFPSIVFVSNERCAWNSHHMAATERRCSLLSSKGLF 60
           MLLFTNVLPTSYFFPT FGCL+P VFPSIVFVS  RC      MAATERRCSLLSSKG+F
Sbjct: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVS--RCG----GMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSGF+RRCK LD EG+FALEAEILEFMESSRNP AFPSKKDLIEAGREDLVDAIVKKG
Sbjct: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFAT----AASS 180
           GWLCLGWNL++EEDGGI Q YLEDRESILA  WDSFGEFNSFKSDERQRFAT     ASS
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSASFTGRSLETVAEHESGIEGMLSRLEKERNAAFGACKNDLHPETTAKETVADQQRST 240
           STS+SFT RS ETV EHESGIEGML+RLE+ERN  FG  KN LHPETT KETVAD Q   
Sbjct: 181 STSSSFTSRSQETVVEHESGIEGMLNRLERERNVTFGTGKNCLHPETTVKETVADLQEIP 240

Query: 241 ISTPLSAKEGKWNDLEGYLSQTSSPGPVQINGLGGSVSSEMQRTWSTFKTDLQDGDFKGS 300
           ISTPLSAKEGKWN+L  YLS+ SS  PVQING GGSVSSE+Q TWSTFKTDL DGDFKGS
Sbjct: 241 ISTPLSAKEGKWNNLGSYLSKNSS--PVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGS 300

Query: 301 EIALSEGRSKEVLDVLRYETLGSRGDLTNSNSGEEEMDHTQIQRHLQNLQLELSSVIGSL 360
           E+ALSEGRS+EVLDVLRYETLGSRGD TNSNSGEEEM H QIQRH+QNLQLELSSVIGSL
Sbjct: 301 EMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELSSVIGSL 360

Query: 361 RSNAFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRARLAVVEGKMALTIIDA 420
           RSN+FASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRA++AVVEGKMALTIIDA
Sbjct: 361 RSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDA 420

Query: 421 QKVTEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSL 480
           QKV EEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKME+SSTGVFSL
Sbjct: 421 QKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSL 480

Query: 481 FLKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLIIT 518
           FLKLYPGKYEIKFIVDGQWKIDPLRPIV S+GYENNLLI+T
Sbjct: 481 FLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 513

BLAST of Tan0014893 vs. ExPASy TrEMBL
Match: A0A6J1HT44 (protein PTST homolog 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467545 PE=4 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 4.2e-247
Identity = 455/552 (82.43%), Postives = 478/552 (86.59%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTAFGCLTPCVFPSIVFVSNERCAWNSHHMAATERRCSLLSSKGLF 60
           MLLFTN LPTSYFFPT FGCL+PC FPS VFVS  RC  NS+HMAATERRCSLLSSKG+F
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFVS--RCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSGF+RRCKGLDSEG+FALEAEILEFMESSRNP AFPSKKDLIEAGREDLVDAIVKKG
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFAT----AASS 180
           GWLCLGWNL++EEDGGI Q YLEDRESILA  WDSFGEFNSFKSDERQRFAT     ASS
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSASFTGRS-------------------------------LETVAEHESGIEGMLSRLE 240
           STS+SFT RS                                ETVAEHESGIEGML+RLE
Sbjct: 181 STSSSFTSRSQVFYSMPMKRNLIFYDNKMLCQDVKDTNFTNRETVAEHESGIEGMLNRLE 240

Query: 241 KERNAAFGACKNDLHPETTAKETVADQQRSTISTPLSAKEGKWNDLEGYLSQTSSPGPVQ 300
           +ERNA FG  KN LH ETTAKETVAD Q   ISTPLSAKEGKW +L  YLS+ SS  PVQ
Sbjct: 241 RERNATFGTGKNGLHSETTAKETVADLQEIPISTPLSAKEGKWTNLGSYLSKNSS--PVQ 300

Query: 301 INGLGGSVSSEMQRTWSTFKTDLQDGDFKGSEIALSEGRSKEVLDVLRYETLGSRGDLTN 360
           ING GGSVSSE+QRTWSTFKTDLQDGDFKGSE+ALSE RS+EVLDVLRYETLGSRGD TN
Sbjct: 301 INGQGGSVSSEVQRTWSTFKTDLQDGDFKGSEMALSEVRSREVLDVLRYETLGSRGDPTN 360

Query: 361 SNSGEEEMDHTQIQRHLQNLQLELSSVIGSLRSNAFASEKGQSKSSDDLLELSDAFEFQE 420
           SNSGEEEM H QIQR++QNLQLELSS IGSLRSNAFASEKGQSK SDDLLELSDAFEFQE
Sbjct: 361 SNSGEEEMSHNQIQRYIQNLQLELSSAIGSLRSNAFASEKGQSKPSDDLLELSDAFEFQE 420

Query: 421 NEILNAKDKLRSIRARLAVVEGKMALTIIDAQKVTEEKQKRINCARRALQFLRTACVVWP 480
           NEILNAKDKLRSIRA++AVVEGKMALTIIDAQKV EEKQKRINCARRALQFLRTACVVWP
Sbjct: 421 NEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWP 480

Query: 481 NSAAEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVN 518
           NSAAEVLLVGSFDGWSTQRKME+SSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV+
Sbjct: 481 NSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVS 540

BLAST of Tan0014893 vs. ExPASy TrEMBL
Match: A0A6J1FZI9 (protein PTST homolog 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449347 PE=4 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 2.4e-242
Identity = 452/552 (81.88%), Postives = 473/552 (85.69%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTAFGCLTPCVFPSIVFVSNERCAWNSHHMAATERRCSLLSSKGLF 60
           MLLFTNVLPTSYFFPT FGCL+P VFPSIVFVS  RC      MAATERRCSLLSSKG+F
Sbjct: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVS--RCG----GMAATERRCSLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSGF+RRCK LD EG+FALEAEILEFMESSRNP AFPSKKDLIEAGREDLVDAIVKKG
Sbjct: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFAT----AASS 180
           GWLCLGWNL++EEDGGI Q YLEDRESILA  WDSFGEFNSFKSDERQRFAT     ASS
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSASFTGRS-------------------------------LETVAEHESGIEGMLSRLE 240
           STS+SFT RS                                ETV EHESGIEGML+RLE
Sbjct: 181 STSSSFTSRSQVCYSMPMKGTLNFYDNKMLCQDVNDTNFTNRETVVEHESGIEGMLNRLE 240

Query: 241 KERNAAFGACKNDLHPETTAKETVADQQRSTISTPLSAKEGKWNDLEGYLSQTSSPGPVQ 300
           +ERN  FG  KN LHPETT KETVAD Q   ISTPLSAKEGKWN+L  YLS+ SS  PVQ
Sbjct: 241 RERNVTFGTGKNCLHPETTVKETVADLQEIPISTPLSAKEGKWNNLGSYLSKNSS--PVQ 300

Query: 301 INGLGGSVSSEMQRTWSTFKTDLQDGDFKGSEIALSEGRSKEVLDVLRYETLGSRGDLTN 360
           ING GGSVSSE+Q TWSTFKTDL DGDFKGSE+ALSEGRS+EVLDVLRYETLGSRGD TN
Sbjct: 301 INGQGGSVSSEVQGTWSTFKTDLPDGDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTN 360

Query: 361 SNSGEEEMDHTQIQRHLQNLQLELSSVIGSLRSNAFASEKGQSKSSDDLLELSDAFEFQE 420
           SNSGEEEM H QIQRH+QNLQLELSSVIGSLRSN+FASEKGQSKSSDDLLELSDAFEFQE
Sbjct: 361 SNSGEEEMSHNQIQRHIQNLQLELSSVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQE 420

Query: 421 NEILNAKDKLRSIRARLAVVEGKMALTIIDAQKVTEEKQKRINCARRALQFLRTACVVWP 480
           NEILNAKDKLRSIRA++AVVEGKMALTIIDAQKV EEKQKRINCARRALQFLRTACVVWP
Sbjct: 421 NEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWP 480

Query: 481 NSAAEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVN 518
           NSAAEVLLVGSFDGWSTQRKME+SSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV 
Sbjct: 481 NSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVR 540

BLAST of Tan0014893 vs. ExPASy TrEMBL
Match: A0A6J1DCK2 (uncharacterized protein LOC111019111 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019111 PE=4 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.4e-226
Identity = 411/521 (78.89%), Postives = 450/521 (86.37%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTAFGCLTPCVFPSIVFVSNERCAWNSHHMAATERRCSLLSSKGLF 60
           ML+FTN LPT  FFP+ FGCL+PCVFP++VFVSN RC WNSHH AATER C LLSSKG+F
Sbjct: 1   MLVFTNALPTFLFFPSPFGCLSPCVFPNVVFVSNSRCGWNSHHTAATERGCVLLSSKGMF 60

Query: 61  YGCSGFLRRCKGLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSG +RRCKGLDSEGDFALEA+ILEFM+SS+NP AFPSKKDLIEAGREDLVDAI KKG
Sbjct: 61  YGCSGVVRRCKGLDSEGDFALEAKILEFMKSSKNPEAFPSKKDLIEAGREDLVDAIAKKG 120

Query: 121 GWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFATA----ASS 180
           GWLCLGWNLDEE D G+GQ+  EDR SI+A DWDSFGE N+ + DER RFA A    ASS
Sbjct: 121 GWLCLGWNLDEEVDEGVGQYDFEDRYSIVATDWDSFGESNNSRRDERPRFAAAASPLASS 180

Query: 181 STSASFTGRSLETVAEHESGIEGMLSRLEKERNAAFGACKNDLHPETTAKETVADQQRST 240
           S S+SFTGRSLE VAE ESGIEGMLSRLEKERN  FGA          AKETVAD Q S+
Sbjct: 181 SASSSFTGRSLEIVAEGESGIEGMLSRLEKERNVTFGA------GFPGAKETVADLQGSS 240

Query: 241 ISTPLSAKEGKWNDLEGYLSQTSSPGPVQINGLGGSVSSEMQRTWSTFKTDLQDGDFKGS 300
           ISTPLSAKEGKWN+L G LS  SS  PVQINGLGGSVS E+QR W+T+ T+L+DGDF+ S
Sbjct: 241 ISTPLSAKEGKWNNLGGSLSHNSS--PVQINGLGGSVSPEVQRIWNTYNTELRDGDFEAS 300

Query: 301 EIALSEGRSKEVLDVLRYETLGSRGDLTNSNSGEEEMDHTQIQRHLQNLQLELSSVIGSL 360
           E AL  GR  EVLDV RY TLGS GDL  SN GEEEM++ QI+RHLQNL+LELSS++GSL
Sbjct: 301 ENALRNGRGGEVLDVSRYGTLGSGGDLIKSNPGEEEMNYHQIRRHLQNLELELSSMVGSL 360

Query: 361 RSNAFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRARLAVVEGKMALTIIDA 420
           RSN FASEKGQS+S+DD LELSDA+EFQENEILNAKDKLRSIRA+LAVVEGKMA+TIIDA
Sbjct: 361 RSNTFASEKGQSRSADDFLELSDAYEFQENEILNAKDKLRSIRAKLAVVEGKMAMTIIDA 420

Query: 421 QKVTEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSL 480
           QKV EEKQKRINCARRALQ LRTACVVWPNSA+EV LVGSFDGW+TQRKMEKSSTGVFSL
Sbjct: 421 QKVIEEKQKRINCARRALQVLRTACVVWPNSASEVFLVGSFDGWATQRKMEKSSTGVFSL 480

Query: 481 FLKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLIIT 518
           FLKLYPGKYEIKFIVDGQW+IDPLRPIVNS+GYENNLLIIT
Sbjct: 481 FLKLYPGKYEIKFIVDGQWRIDPLRPIVNSSGYENNLLIIT 513

BLAST of Tan0014893 vs. TAIR 10
Match: AT1G27070.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 360.1 bits (923), Expect = 2.9e-99
Identity = 216/472 (45.76%), Postives = 291/472 (61.65%), Query Frame = 0

Query: 63  CSGFLRRCK----GLDSEGDFALEAEILEFMESSRNPGAFPSKKDLIEAGREDLVDAIVK 122
           C+G  R C     G DSEG   LE EILEFM++S  PG FPSKKDLI +GR DLV+ IV 
Sbjct: 72  CNGVSRMCNKRNLGWDSEGSKDLETEILEFMKNSEKPGMFPSKKDLIRSGRFDLVERIVN 131

Query: 123 KGGWLCLGWNLDEEEDGGIGQFYLEDRESILAADWDSFGEFNSFKSDERQRFATAASSST 182
           +GGWL +GW+LDE+E+       +   E++   D     +  +  S E  +         
Sbjct: 132 QGGWLSMGWDLDEQEE------KVRVNENVTPQDLHIEKQLPNCNSPEMDKTLNHGDLDL 191

Query: 183 SASFTGRSLETVAEHESGIEGMLSRLEKERNAAFGACKNDLHPETTAKETVADQ----QR 242
           S++ +  + +  + ++SGIEG+L+RLEKERN + G    +      A   ++        
Sbjct: 192 SSNLSSSTEQVESRNDSGIEGILTRLEKERNLSLGISVRENGKSNGAMHDISPNGSVPWS 251

Query: 243 STISTPLSAKEGKWNDLEGYLSQTSSPGPVQIN---GLGGSVSSEMQRTWSTFKTDLQDG 302
           S I T    +E   +   G  +Q+   G   ++   GL  S +SE  RTWS  +    D 
Sbjct: 252 SRIVTASEIQEVDGSRGSGEYAQSRYQGAKSVSGKPGLSDSPTSETWRTWSMRRAGFTDE 311

Query: 303 DFKGSEIALSEGRSKEVLDVLRYETLGSRGDLTNSN----SGEEEMDHTQIQRHLQNLQL 362
           DF+ +EI+     S  +  V + +T    GD  N      S  E+++ T I+  LQ LQ 
Sbjct: 312 DFEAAEIS-----SSGLTGVKKDDTKKDSGDSMNGKDRIASSSEDVNKTHIKHRLQQLQS 371

Query: 363 ELSSVIGSLRS--NAFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRARLAVV 422
           ELSSV+ SLRS  +   + K    ++ +L  LSD +E++ENEI++A++KLRS RA+LAV+
Sbjct: 372 ELSSVLHSLRSPPDKVVTSKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAKLAVL 431

Query: 423 EGKMALTIIDAQKVTEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRK 482
           EGKMA+ IIDAQ++  EKQ+RI+ A RAL+ LRTA +VWPNSA+EVLL GSFDGWSTQRK
Sbjct: 432 EGKMAMAIIDAQRIVREKQRRIDHASRALRLLRTASIVWPNSASEVLLTGSFDGWSTQRK 491

Query: 483 MEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLIIT 518
           M+K+  GVFSL LKLYPGKYEIKFIVDGQWK+DPLRPIV S GYENNLLII+
Sbjct: 492 MKKAENGVFSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLIIS 532

BLAST of Tan0014893 vs. TAIR 10
Match: AT5G03420.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 73.6 bits (179), Expect = 5.4e-13
Identity = 44/137 (32.12%), Postives = 69/137 (50.36%), Query Frame = 0

Query: 384 QENEILNAKDKLRSIRARLAVVEGKMALTIIDAQKVTEEKQKRINCARRALQFLRTACVV 443
           +E E+   K+++   +  L+V++ +    I  AQ +  EK+  +  A  +L  L+   + 
Sbjct: 457 KELELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEESLSGLQEVVIE 516

Query: 444 WPNSAAEVLLVGSFDGWSTQRKMEKSST------GVFSLFLKLYPGKYEIKFIVDGQWKI 503
           +      V + GSF+GW  +  ME  ++        +S  L LYPG YEIKFIVDGQW  
Sbjct: 517 YCGDGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYEIKFIVDGQWIT 576

Query: 504 DPLRPIVNSNGYENNLL 515
           DP +  V      NN+L
Sbjct: 577 DPQKDSVTRGHISNNIL 593

BLAST of Tan0014893 vs. TAIR 10
Match: AT5G39790.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 70.1 bits (170), Expect = 5.9e-12
Identity = 50/160 (31.25%), Postives = 77/160 (48.12%), Query Frame = 0

Query: 384 QENEILNAKDKLRSIR-------------ARLAVVEGKM----------ALTIIDA-QKV 443
           QE+EI N K +L  +              A L + +G             L+ +DA QK 
Sbjct: 118 QEHEITNIKTELALMELEVQALVKLAEEIANLGIPQGSRKISGKYIQSHLLSRLDAVQKK 177

Query: 444 TEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSLF-- 503
            +E+ K +  A+      +   V W   A  V ++GSFDGWS +  +    + +F+ F  
Sbjct: 178 MKEQIKGVEAAQS-----KEVHVFWIGMAESVQVMGSFDGWSQREDLSPEYSALFTKFST 237

Query: 504 -LKLYPGKYEIKFIVDGQWKIDPLRPIVNSNGYENNLLII 517
            L L PG+YE+KF+VDG+W+I P  P       ENN+L++
Sbjct: 238 TLFLRPGRYEMKFLVDGEWQISPEFPTSGEGLMENNVLVV 272

BLAST of Tan0014893 vs. TAIR 10
Match: AT4G16360.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 53.5 bits (127), Expect = 5.8e-07
Identity = 22/66 (33.33%), Postives = 38/66 (57.58%), Query Frame = 0

Query: 442 VVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLR 501
           + W +   E+ + GS+D W T+ ++++S    F++   L  G YE +FIVDGQW+  P  
Sbjct: 76  ITWCHGGKEIAVEGSWDNWKTRSRLQRSGKD-FTIMKVLPSGVYEYRFIVDGQWRHAPEL 135

Query: 502 PIVNSN 508
           P+   +
Sbjct: 136 PLARDD 140

BLAST of Tan0014893 vs. TAIR 10
Match: AT4G16360.3 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 53.5 bits (127), Expect = 5.8e-07
Identity = 22/66 (33.33%), Postives = 38/66 (57.58%), Query Frame = 0

Query: 442 VVWPNSAAEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLR 501
           + W +   E+ + GS+D W T+ ++++S    F++   L  G YE +FIVDGQW+  P  
Sbjct: 78  ITWCHGGKEIAVEGSWDNWKTRSRLQRSGKD-FTIMKVLPSGVYEYRFIVDGQWRHAPEL 137

Query: 502 PIVNSN 508
           P+   +
Sbjct: 138 PLARDD 142