Tan0014868 (gene) Snake gourd v1

Overview
NameTan0014868
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionprotein kinase PINOID 2-like
LocationLG08: 1979177 .. 1981793 (-)
RNA-Seq ExpressionTan0014868
SyntenyTan0014868
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTCCTCTGCAAGAAACCGTTTTCCGCCATGGCCGCCATAGCCCGAGACGAGTCCGACTACGACAGCAGTTGCTCCTCCATCACCGTCCCAGACTCGAGCCGCAGCTGGATGAGCAACCTCAGCTTCGGGAGCCGGCGTCGGAGCAGCAGCTCCGGCGCCGACGCCGGAAGCTCTCCCCACAAACCCCACAAAGCAAACCAAGTAGCGTGGGAGGCGATGCGGTGGCTCCGGGGAGTGAAAGGGCGTGTGGGACTGGAGCACTTCCGACTGCTCCGGCGGCTGGGGAGCGGAGACATTGGGAATGTTTATCTGTGCGAAATAAGGAATCCGATGGTGGGTCTGGCGCAGTGCTTTTACGCAATGAAAGTGGTTGACAGAGAAGCGCTTTCAGTTAGGAATAAGGAGAGAAGAGCGGATATGGAGAAGGAGATTCTTAAAGTGATTGATCATCCATTTTTGCCCACGCTTTATGCAGAGTTTGAAGCTTCTCATTACTCGTGTTTGGTTATGGAGTTTTGTCCCGGTGGAGATTTGTATGCCGCTCGCCAACGCCTCCCCGGAAAACGATTCTCCATTTCTTCTGCTAAGTATGTTCTTCTAATTTCCTTTTAACTATTTTTTTTTTTAAGAATTAAGCTTAAAAATACTGCCCCTTTAAAAAATTTAAATTGTATAATTTTTAAAAAACTATTTTCCTTTTTAAATTTGGTTAGAACTTTAATGTTAATTTAGTTAAGATGCGAAATAAGTATAATTTTCAAAAAATAAAAAATAAACTTAAATTAATTCATTTTGATTCATTTTTTAACCCGAGTTTCTTTATAGAAATTATTGGATGAGGTAGTTGCAATAAAGCGAGAATTTTGCACTATTAAATTATTTTGGTGTTTGTACTTCTAAATTTATTTTTATTTTTTATTTTTGAGTTTTATATTATTTATTTTACTTTTTTTTAATCATTATTATTTATTTTACTTGGAGTATATGAGTACACATATCTTAATTAATTGATAACTAGTTTTAAGTTTGATTTTTACTTTTTAGGATTTTTTTAGAAGGTACATGGCAATTTAAAGTTGAGCTATGTTTTATTTTTAGAATTAATATACCTACATATTTGGGCCTATCATTAAGAGTCTTGACTACTTTTAATAATTTGACAACTATTTATTTCTTTTTTGAAAAAAAATAAATTATTCTTTTTATCCCTCTATCTTTTTTTTTTCAATTATCGAAAACATAATTTGACAACGTACTATTTATTCTTTTTTATCCCTATGACATAGAATTAAAAAATAAAAACAATATTTTTTTCCCTTAATACATGCATTAGGTTGGTTAAAGTATGTATTAAACATGACAACTATAAAGGTGGAAATGAATTGTTTTAGTAATTTAAAATAAATATATAAGACTAACTCACTTATAGTTATAGCTTAACTAGTTAAACCAATGGAATCATCAACTAAGATAAAGGGATTTATGGAATAATCAATATTTTTCTTCTTTGATTTCGAGACAAATAAGACGAAATAAAATTGAAAATAATTAATTTTCTTTCGAATGTAAAAATGTGTAATGTCTCGGGCAATTTATTGGGAGGTGATAAAGATTAGAAATTCAAATTATTGATAAATACTAATGGTAAAATGGTTTTTTTGTTTTTTTTTTAATATTAATTTATAGGTTTTATGCGGCGGAGATAATTTTAGGCCTAGAGTATTTGCACATGATGGGCATAGTTTACAGAGATTTGAAGCCGGAAAACGTGCTGGTTCGAGAAGACGGCCACATAATGCTGTCGGATTTCGATCTCTGCCTCAAATGCGACGTCGTTCCAAGGCTTATCCGCTGCAGAACCACCGCATCCGACGCCGCCGAACGCCACGTCAAAACCTACTCCACGTCATCAACCACCTCCTGCGCCGCCGCCCCAATCCAGCCTGTCCTCTCCTGTTTCTCCCTCGCCCGCCGCAGAAAATCCACCACCGTCACCACCATCACCGAACACGCAGCCGCCGCCGCTGCCGACGACGACGGCGACGGTGGCGAGTTCGACCCAGAGCCGGAATTAGTAGCGGAGCCGATCAACGCCCGGTCGAAATCCTTCGTGGGAACCCACGAGTATTTAGCGCCGGAGGTGATCTCCGGGCAGGGACACGGCAGCGCCGTCGATTGGTGGACGTTGGGAATTTTCCTATACGAAATGTTGTACGGAAAAACCCCATTTAAAGGGGAAAGTAACGAGAAAACCCTAATCAACATTTTGAAGCAGCCATTGGCGTTTCCAAGAATTGGGTTTAGTAATAGTAGCAAAGAGTATGAAGAAATGGTGAAAGTTGAAGATCTAATCAAGAAGCTTTTGGTGAAGAATCCCAAAAAGAGAATTGGAAGCTTTAAGGGTTCGGTTGAAATCAAAAGACATGAGTTTTTCAAAGGGGTAAATTGGGCTTTAATCCGCTCGGTTCGACCTCCCCAAATCCCCAACTCCGACCATCTACTTCACAACAATATTAATCCATCTAAATACTCTTCTAATTACTTACCTAAATTAAGCAAGAAGCAACGTGAACAACCCTATCAAATCCCTCCTCACCACTTTGATTACTTCTAA

mRNA sequence

TTTTTCCTCTGCAAGAAACCGTTTTCCGCCATGGCCGCCATAGCCCGAGACGAGTCCGACTACGACAGCAGTTGCTCCTCCATCACCGTCCCAGACTCGAGCCGCAGCTGGATGAGCAACCTCAGCTTCGGGAGCCGGCGTCGGAGCAGCAGCTCCGGCGCCGACGCCGGAAGCTCTCCCCACAAACCCCACAAAGCAAACCAAGTAGCGTGGGAGGCGATGCGGTGGCTCCGGGGAGTGAAAGGGCGTGTGGGACTGGAGCACTTCCGACTGCTCCGGCGGCTGGGGAGCGGAGACATTGGGAATGTTTATCTGTGCGAAATAAGGAATCCGATGGTGGGTCTGGCGCAGTGCTTTTACGCAATGAAAGTGGTTGACAGAGAAGCGCTTTCAGTTAGGAATAAGGAGAGAAGAGCGGATATGGAGAAGGAGATTCTTAAAGTGATTGATCATCCATTTTTGCCCACGCTTTATGCAGAGTTTGAAGCTTCTCATTACTCGTGTTTGGTTATGGAGTTTTGTCCCGGTGGAGATTTGTATGCCGCTCGCCAACGCCTCCCCGGAAAACGATTCTCCATTTCTTCTGCTAAGTTTTATGCGGCGGAGATAATTTTAGGCCTAGAGTATTTGCACATGATGGGCATAGTTTACAGAGATTTGAAGCCGGAAAACGTGCTGGTTCGAGAAGACGGCCACATAATGCTGTCGGATTTCGATCTCTGCCTCAAATGCGACGTCGTTCCAAGGCTTATCCGCTGCAGAACCACCGCATCCGACGCCGCCGAACGCCACGTCAAAACCTACTCCACGTCATCAACCACCTCCTGCGCCGCCGCCCCAATCCAGCCTGTCCTCTCCTGTTTCTCCCTCGCCCGCCGCAGAAAATCCACCACCGTCACCACCATCACCGAACACGCAGCCGCCGCCGCTGCCGACGACGACGGCGACGGTGGCGAGTTCGACCCAGAGCCGGAATTAGTAGCGGAGCCGATCAACGCCCGGTCGAAATCCTTCGTGGGAACCCACGAGTATTTAGCGCCGGAGGTGATCTCCGGGCAGGGACACGGCAGCGCCGTCGATTGGTGGACGTTGGGAATTTTCCTATACGAAATGTTGTACGGAAAAACCCCATTTAAAGGGGAAAGTAACGAGAAAACCCTAATCAACATTTTGAAGCAGCCATTGGCGTTTCCAAGAATTGGGTTTAGTAATAGTAGCAAAGAGTATGAAGAAATGGTGAAAGTTGAAGATCTAATCAAGAAGCTTTTGGTGAAGAATCCCAAAAAGAGAATTGGAAGCTTTAAGGGTTCGGTTGAAATCAAAAGACATGAGTTTTTCAAAGGGGTAAATTGGGCTTTAATCCGCTCGGTTCGACCTCCCCAAATCCCCAACTCCGACCATCTACTTCACAACAATATTAATCCATCTAAATACTCTTCTAATTACTTACCTAAATTAAGCAAGAAGCAACGTGAACAACCCTATCAAATCCCTCCTCACCACTTTGATTACTTCTAA

Coding sequence (CDS)

ATGGCCGCCATAGCCCGAGACGAGTCCGACTACGACAGCAGTTGCTCCTCCATCACCGTCCCAGACTCGAGCCGCAGCTGGATGAGCAACCTCAGCTTCGGGAGCCGGCGTCGGAGCAGCAGCTCCGGCGCCGACGCCGGAAGCTCTCCCCACAAACCCCACAAAGCAAACCAAGTAGCGTGGGAGGCGATGCGGTGGCTCCGGGGAGTGAAAGGGCGTGTGGGACTGGAGCACTTCCGACTGCTCCGGCGGCTGGGGAGCGGAGACATTGGGAATGTTTATCTGTGCGAAATAAGGAATCCGATGGTGGGTCTGGCGCAGTGCTTTTACGCAATGAAAGTGGTTGACAGAGAAGCGCTTTCAGTTAGGAATAAGGAGAGAAGAGCGGATATGGAGAAGGAGATTCTTAAAGTGATTGATCATCCATTTTTGCCCACGCTTTATGCAGAGTTTGAAGCTTCTCATTACTCGTGTTTGGTTATGGAGTTTTGTCCCGGTGGAGATTTGTATGCCGCTCGCCAACGCCTCCCCGGAAAACGATTCTCCATTTCTTCTGCTAAGTTTTATGCGGCGGAGATAATTTTAGGCCTAGAGTATTTGCACATGATGGGCATAGTTTACAGAGATTTGAAGCCGGAAAACGTGCTGGTTCGAGAAGACGGCCACATAATGCTGTCGGATTTCGATCTCTGCCTCAAATGCGACGTCGTTCCAAGGCTTATCCGCTGCAGAACCACCGCATCCGACGCCGCCGAACGCCACGTCAAAACCTACTCCACGTCATCAACCACCTCCTGCGCCGCCGCCCCAATCCAGCCTGTCCTCTCCTGTTTCTCCCTCGCCCGCCGCAGAAAATCCACCACCGTCACCACCATCACCGAACACGCAGCCGCCGCCGCTGCCGACGACGACGGCGACGGTGGCGAGTTCGACCCAGAGCCGGAATTAGTAGCGGAGCCGATCAACGCCCGGTCGAAATCCTTCGTGGGAACCCACGAGTATTTAGCGCCGGAGGTGATCTCCGGGCAGGGACACGGCAGCGCCGTCGATTGGTGGACGTTGGGAATTTTCCTATACGAAATGTTGTACGGAAAAACCCCATTTAAAGGGGAAAGTAACGAGAAAACCCTAATCAACATTTTGAAGCAGCCATTGGCGTTTCCAAGAATTGGGTTTAGTAATAGTAGCAAAGAGTATGAAGAAATGGTGAAAGTTGAAGATCTAATCAAGAAGCTTTTGGTGAAGAATCCCAAAAAGAGAATTGGAAGCTTTAAGGGTTCGGTTGAAATCAAAAGACATGAGTTTTTCAAAGGGGTAAATTGGGCTTTAATCCGCTCGGTTCGACCTCCCCAAATCCCCAACTCCGACCATCTACTTCACAACAATATTAATCCATCTAAATACTCTTCTAATTACTTACCTAAATTAAGCAAGAAGCAACGTGAACAACCCTATCAAATCCCTCCTCACCACTTTGATTACTTCTAA

Protein sequence

MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREALSVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKRFSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRLIRCRTTASDAAERHVKTYSTSSTTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAAAADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPSKYSSNYLPKLSKKQREQPYQIPPHHFDYF
Homology
BLAST of Tan0014868 vs. ExPASy Swiss-Prot
Match: Q94E49 (Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 9.1e-164
Identity = 303/510 (59.41%), Postives = 370/510 (72.55%), Query Frame = 0

Query: 1   MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSSGAD--AGSSPHKPHKANQ 60
           MAAI ++ESDYDSS SS+T PDS RSW+S++   S   + S G D  A S  +KPHKANQ
Sbjct: 1   MAAI-KEESDYDSSRSSLTAPDSRRSWISDIGSSSSVSARSFGGDTPASSCRYKPHKANQ 60

Query: 61  VAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQ------CFYAM 120
             WEA+R LR   GRVGLEHFRL+RRLGSGD+GNVYLC +R P    +       C YAM
Sbjct: 61  AEWEAIRRLRAGAGRVGLEHFRLVRRLGSGDLGNVYLCRLREPWSSSSMTTTAGGCLYAM 120

Query: 121 KVVDREALSVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAA 180
           KVVD++AL+ R K RRA++E++IL+ +DHPFLPTLYA+FEASHY+CLVMEFCPGGDL+ A
Sbjct: 121 KVVDKDALAFRKKLRRAEVERDILRTLDHPFLPTLYADFEASHYACLVMEFCPGGDLHVA 180

Query: 181 RQRLPGKRFSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCL 240
           RQR PG+RF++SS +FY AE +L LEYLHMMG+VYRDLKPENVLVR DGHIMLSDFDL L
Sbjct: 181 RQRQPGRRFTVSSTRFYVAETVLALEYLHMMGVVYRDLKPENVLVRGDGHIMLSDFDLSL 240

Query: 241 KCDVVPRLIRCRTTASDAAERHVKTYSTSSTTSCAAAPIQPVLSCFSLARRRKSTTVTTI 300
           KCDVVP+L+R   +A+   +  +   S     SC    IQPVLSC       K       
Sbjct: 241 KCDVVPKLLRPARSAAAGGKPPLPPPS-----SCVPPTIQPVLSCI-FRGVHKCHHAKEC 300

Query: 301 TEHAAAAAADDDGDGGEFDPEPE------LVAEPINARSKSFVGTHEYLAPEVISGQGHG 360
               AAA  + DGDG + + E E      +V EP+ ARSKSFVGTHEYLAPEVISGQGHG
Sbjct: 301 AGGGAAAGNNGDGDGNDEEAETETAEPEVVVVEPVAARSKSFVGTHEYLAPEVISGQGHG 360

Query: 361 SAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVE 420
           SAVDWWTLG+F+YEMLYG+TPFKGESNEKTLINI+KQP+ FPR+  + ++ E+EEM   +
Sbjct: 361 SAVDWWTLGVFMYEMLYGRTPFKGESNEKTLINIIKQPVTFPRLAGAAAAGEWEEMKTAQ 420

Query: 421 DLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPS 480
           DL+ +LL KNPKKR+GS  GS E+KRH FFKGVNWAL+RSVRPP++P           P 
Sbjct: 421 DLMLQLLAKNPKKRLGSTMGSAEVKRHPFFKGVNWALVRSVRPPEVP----------APP 480

Query: 481 KYSSNYLPKLSKKQREQPYQI-PPHHFDYF 496
             +   +  +SKK+R++PY   P +HFDYF
Sbjct: 481 APAPKKVMTMSKKERQEPYNYRPENHFDYF 493

BLAST of Tan0014868 vs. ExPASy Swiss-Prot
Match: Q64FQ2 (Protein kinase PINOID 2 OS=Arabidopsis thaliana OX=3702 GN=PID2 PE=1 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 1.9e-153
Identity = 305/528 (57.77%), Postives = 367/528 (69.51%), Query Frame = 0

Query: 7   DESDYDSSCSSITVPDSSR--SWMSNLSFGSRRRSSSSG-ADAGSSPH---KPHKANQVA 66
           +ESDY+SS      PDSSR  SW+S+    S   SS S  ++ G + +   KPHKANQVA
Sbjct: 11  NESDYESSTVG---PDSSRRTSWLSSSFTASPSCSSISHLSNHGLNSYNQSKPHKANQVA 70

Query: 67  WEAMRWLRGVKGR-VGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREA 126
           WEAM  LR   GR VGLEHFRLL+RLGSGDIG+VYLC+IR         FYAMKVVD+EA
Sbjct: 71  WEAMARLRRCCGRAVGLEHFRLLKRLGSGDIGSVYLCQIRG---SPETAFYAMKVVDKEA 130

Query: 127 LSVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGK 186
           ++V+ K  RA+MEK+IL ++DHPF PTLYA FEASHYS LVME+CPGGDLYA R R P K
Sbjct: 131 VAVKKKLGRAEMEKKILGMLDHPFCPTLYAAFEASHYSFLVMEYCPGGDLYAVRLRQPSK 190

Query: 187 RFSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPR 246
           RF+ISS +FYAAE ++ LEYLHMMGIVYRDLKPENVL+REDGH+MLSDFDL  KCDVVP+
Sbjct: 191 RFTISSTRFYAAETLVALEYLHMMGIVYRDLKPENVLIREDGHVMLSDFDLSFKCDVVPQ 250

Query: 247 LI--RCRTTASDAAERHVKTYSTSSTTSCAAAPIQPVLSCFS--LARRRKSTTVTTITEH 306
            +    R       +  +      ST SC   P+ PV+SCFS   +RRRK   VTT    
Sbjct: 251 FLSDNDRDRGHQEDDDDISIRRKCSTPSCTTTPLNPVISCFSPTSSRRRKKNVVTTTIHE 310

Query: 307 AAAAAADD-------------------------DGDGGEFDPEPELVAEPINARSKSFVG 366
            AA  +D                          D  GG     P + AEPINARSKSFVG
Sbjct: 311 NAAGTSDSVKSNDVSRTFSRSPSSCSRVSNGLRDISGG----CPSIFAEPINARSKSFVG 370

Query: 367 THEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINILKQPLAFPRI 426
           THEYLAPEVISGQGHGSAVDWWT GIFLYEM++G+TPFKG++NEKTL+NILK PL FP++
Sbjct: 371 THEYLAPEVISGQGHGSAVDWWTYGIFLYEMIFGRTPFKGDNNEKTLVNILKAPLTFPKV 430

Query: 427 GFSNSSKEYEEMVKVEDLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPP 486
              NS KEYE+MV  +DLI KLLVKNPKKR+GS KGS+EIKRHEFF+GVNWALIRS++PP
Sbjct: 431 -IVNSPKEYEDMVNAQDLIIKLLVKNPKKRLGSLKGSIEIKRHEFFEGVNWALIRSIKPP 490

Query: 487 QIPNSDHLLHNNINPSKYSSNYLP---KLSKKQREQPYQIPPHHFDYF 496
            +P  +   H     ++  + YLP    +S+K+R +PY +  ++FDYF
Sbjct: 491 WVP-KEETSHKTKGDNRSVNYYLPPRFMMSRKERNEPYHV-SNYFDYF 525

BLAST of Tan0014868 vs. ExPASy Swiss-Prot
Match: Q9SUA3 (Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1 PE=1 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 2.6e-118
Identity = 216/417 (51.80%), Postives = 277/417 (66.43%), Query Frame = 0

Query: 39  SSSSGADAGSSPHKPHKANQVAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEI 98
           S  S   + S  +KPHKAN V WEA++ +R   G +GL HFRLL+RLG GDIG V+L E+
Sbjct: 83  SDESTCSSFSGNNKPHKANDVRWEAIQAVRTKHGVLGLNHFRLLKRLGCGDIGTVHLAEL 142

Query: 99  RNPMVGLAQCFYAMKVVDREALSVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSC 158
                   +CF+AMKV+D+ AL+ R K  RA  E+EIL+ +DHPFLPTLY+ FE   +SC
Sbjct: 143 HG-----TRCFFAMKVMDKGALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSC 202

Query: 159 LVMEFCPGGDLYAARQRLPGKRFSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVR 218
           LVMEFCPGGDL+  RQR PGKRFS  +AKFY AE++L +EYLHM+GI+YRDLKPENVLVR
Sbjct: 203 LVMEFCPGGDLHTLRQRQPGKRFSEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVR 262

Query: 219 EDGHIMLSDFDLCLKCDVVPRLIRCRTTASDAAER--HVKTYSTSSTTSCAAAPIQPVLS 278
           +DGH+MLSDFDL L+C V P ++R    AS+  +   +    +     SC +AP      
Sbjct: 263 DDGHVMLSDFDLSLRCTVSPTVVRSTVLASEGQKNSGYCAQPACIQQPSCISAPTTCFSP 322

Query: 279 CFSLARRRKSTTVTTITEHAAAAAADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLA 338
            +  ++ +K   +   T             G +  P PELVAEP +ARS SFVGTHEYLA
Sbjct: 323 RYFSSKSKKDKKMKNET-------------GNQVSPLPELVAEPTSARSMSFVGTHEYLA 382

Query: 339 PEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSS 398
           PE+I G+GHGSAVDWWT GIFLYE+L+GKTPFKG  N  TL N++ QPL FP     + +
Sbjct: 383 PEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPESPVVSFA 442

Query: 399 KEYEEMVKVEDLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIP 454
                     DLI+ LLVK P+ R+   +G+ E+K+H FF+GVNWAL+R   PP+IP
Sbjct: 443 --------ARDLIRSLLVKEPQHRLAYKRGATEMKQHPFFEGVNWALVRCASPPEIP 473

BLAST of Tan0014868 vs. ExPASy Swiss-Prot
Match: Q9FG74 (Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 7.0e-116
Identity = 221/445 (49.66%), Postives = 285/445 (64.04%), Query Frame = 0

Query: 22  DSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVAWEAMRWLRGVKGRVGLEHFRL 81
           +S +S     S  S     S+ +   SS +KPHKAN V WEA++ +R   G +GL HFRL
Sbjct: 52  ESGKSSTCRPSTSSDISDESTCSSFSSSINKPHKANDVRWEAIQAVRTKHGGLGLNHFRL 111

Query: 82  LRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREALSVRNKERRADMEKEILKVIDH 141
           L+RLG GDIG V+L E+        +C++AMKV+D+ AL+ R K  RA  E+EIL+ +DH
Sbjct: 112 LKRLGCGDIGTVHLAELNG-----TRCYFAMKVMDKTALASRKKLLRAQTEREILQCLDH 171

Query: 142 PFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKRFSISSAKFYAAEIILGLEYLH 201
           PFLPTLY+ FE   +SCLVMEFCPGGDL+  RQR PGKRF+  +AKFY AE++L +EYLH
Sbjct: 172 PFLPTLYSHFETEKFSCLVMEFCPGGDLHTLRQRQPGKRFTEQAAKFYVAEVLLAMEYLH 231

Query: 202 MMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRLIRCRTTASDAAERHVKTYSTS 261
           M+GI+YRDLKPENVLVR+DGH+MLSDFDL L+C V   ++R     S+   ++  + S S
Sbjct: 232 MLGIIYRDLKPENVLVRDDGHVMLSDFDLSLRCTVSLSIVRSANVGSEGLSKN--SVSCS 291

Query: 262 STTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAAAADDDGDGGEFDPEPELVAEPI 321
              +C   P     SC S+A           ++         +    +  P PELVAEP 
Sbjct: 292 QQPACIQQP-----SCISMAPTSCFGPRFFSSKSKKDKKPKTENGNHQVTPLPELVAEPT 351

Query: 322 NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINIL 381
            ARS SFVGTHEYLAPE+I G+GHGSAVDWWT GIFLYE+L+GKTPFKG  N  TL N++
Sbjct: 352 GARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVV 411

Query: 382 KQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNW 441
            QPL FP     + +          DLI+ LLVK P+ R+   +G+ EIK+H FF+GVNW
Sbjct: 412 GQPLRFPESPVVSFA--------ARDLIRSLLVKEPQHRLAYKRGATEIKQHPFFEGVNW 471

Query: 442 ALIRSVRPPQIPNSDHLLHNNINPS 467
           AL+R   PP+IP    L   N  P+
Sbjct: 472 ALVRCASPPEIPKPVDLEALNPTPT 476

BLAST of Tan0014868 vs. ExPASy Swiss-Prot
Match: Q39183 (Serine/threonine-protein kinase D6PKL2 OS=Arabidopsis thaliana OX=3702 GN=D6PKL2 PE=1 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 2.7e-115
Identity = 219/433 (50.58%), Postives = 281/433 (64.90%), Query Frame = 0

Query: 22  DSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVAWEAMRWLRGVKGRVGLEHFRL 81
           +S +S +   S  S     SS +   S+ +KPHKAN + WEA++ +R   G +GL HFRL
Sbjct: 134 ESGKSSICRGSTSSDVSDESSCSSFSSTVNKPHKANDLRWEAIQAVRVRDGLLGLSHFRL 193

Query: 82  LRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREALSVRNKERRADMEKEILKVIDH 141
           L+RLG GDIG+VYL E+        +C++AMKV+D+ +L+ R K  RA  E+EIL+ +DH
Sbjct: 194 LKRLGCGDIGSVYLSELSG-----TKCYFAMKVMDKTSLASRKKLLRAQTEREILQSLDH 253

Query: 142 PFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKRFSISSAKFYAAEIILGLEYLH 201
           PFLPTLY  FE   +SCLVMEFCPGGDL+  RQR PGK FS  + KFY AE +L LEYLH
Sbjct: 254 PFLPTLYTHFETEKFSCLVMEFCPGGDLHTLRQRQPGKHFSEQAVKFYIAESLLALEYLH 313

Query: 202 MMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRLIRCRTTASDAAERHVKTYSTS 261
           M+GIVYRDLKPENVLVREDGHIMLSDFDL L+C V P L++     SD   ++V     +
Sbjct: 314 MLGIVYRDLKPENVLVREDGHIMLSDFDLSLRCLVSPTLVKSAAIESDPLRKNVYCVQPA 373

Query: 262 S-TTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAAAADDDGDGGEFDPEPELVAEP 321
               SC         +CFS             +  +       +    +  P PELVAEP
Sbjct: 374 CIEPSCIQPSCTVPTTCFS---------PRLFSSKSKKDRKPKNDTANQVRPLPELVAEP 433

Query: 322 INARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINI 381
            +ARS SFVGTHEYLAPE+I G+GHGSAVDWWT GIFLYE+L+G+TPFKG  N +TL N+
Sbjct: 434 TDARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGRTPFKGSGNRQTLFNV 493

Query: 382 LKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVN 441
           + QPL FP     + +          DLI+ LL+K P++R+G  +G+ E+K+H FF+GVN
Sbjct: 494 VGQPLRFPETPVVSFA--------ARDLIRGLLMKEPQQRLGFKRGATEVKQHPFFEGVN 544

Query: 442 WALIRSVRPPQIP 454
           WALIR   PP+IP
Sbjct: 554 WALIRCATPPEIP 544

BLAST of Tan0014868 vs. NCBI nr
Match: XP_022965690.1 (protein kinase PINOID 2-like [Cucurbita maxima])

HSP 1 Score: 857.1 bits (2213), Expect = 7.7e-245
Identity = 430/496 (86.69%), Postives = 459/496 (92.54%), Query Frame = 0

Query: 1   MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVA 60
           MA I RD+SD+DS+CSS+T+PDSSRSWMSNLSFGS RRSS+S  DA  SP+KPHKANQVA
Sbjct: 1   MAGITRDDSDHDSTCSSLTLPDSSRSWMSNLSFGS-RRSSTSALDAALSPYKPHKANQVA 60

Query: 61  WEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREAL 120
           WEAMRW+R VKGRVGLEHFRLLRR+GSGDIGNVYLCEIRNP+VGLAQC+YAMKVVDREA+
Sbjct: 61  WEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPVVGLAQCYYAMKVVDREAV 120

Query: 121 SVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180
           SVRNK RRADMEKEILK+IDHPFLPTLYA+FEASHYSCLVMEFCPGGDLYAARQRLPGKR
Sbjct: 121 SVRNKGRRADMEKEILKLIDHPFLPTLYAQFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180

Query: 181 FSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRL 240
           F I S KFYAAEIIL LEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVP+L
Sbjct: 181 FPIPSVKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPKL 240

Query: 241 IRCRTTASDAAERHV-KTYSTSSTTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAA 300
           IRCRTT    AERHV KTYSTSSTTSCAAAPIQPVLSCFS+ RRR STT+TTITEHAA +
Sbjct: 241 IRCRTT----AERHVTKTYSTSSTTSCAAAPIQPVLSCFSITRRRNSTTITTITEHAAES 300

Query: 301 AADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360
           AA  D   GEFDPEPEL+AEP+NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY
Sbjct: 301 AATVD---GEFDPEPELIAEPMNARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360

Query: 361 EMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKK 420
           EMLYGKTPFKGE+NEKTL+NILKQPL FPRIG S S+KEYEEMVKVEDLI+KLLVKNPKK
Sbjct: 361 EMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVS-SNKEYEEMVKVEDLIRKLLVKNPKK 420

Query: 421 RIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPSKYSSNYLPKLSKK 480
           RIGS KGSVEIKRHEFFKGVNWALIRSVRPPQ+PNSDHLLH  +  SKY SNY+PKL+KK
Sbjct: 421 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLLHKTLTQSKYPSNYVPKLTKK 480

Query: 481 QREQPYQIPPHHFDYF 496
           QR+QPYQIP  HFDYF
Sbjct: 481 QRQQPYQIPSPHFDYF 487

BLAST of Tan0014868 vs. NCBI nr
Match: KAG7021258.1 (Protein kinase PINOID 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 849.0 bits (2192), Expect = 2.1e-242
Identity = 426/496 (85.89%), Postives = 457/496 (92.14%), Query Frame = 0

Query: 1   MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVA 60
           MA+I RD+SD+DS+ SS+T+PDS+RSW+SNLS  S RRSS+S  DA  SP+KPHKAN VA
Sbjct: 1   MASITRDDSDHDSTSSSLTLPDSTRSWLSNLSISS-RRSSTSALDAALSPYKPHKANHVA 60

Query: 61  WEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREAL 120
           WEAMRW+R VKGRVGLEHFRLLRR+GSGDIGNVYLCEIRNP+VGLAQC+YAMKVVDREA+
Sbjct: 61  WEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPVVGLAQCYYAMKVVDREAV 120

Query: 121 SVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180
           SVRNK RRADMEKEILK+IDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR
Sbjct: 121 SVRNKARRADMEKEILKLIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180

Query: 181 FSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRL 240
           F ISSAKFYAAEIIL LEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVP+L
Sbjct: 181 FPISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPKL 240

Query: 241 IRCRTTASDAAERHV-KTYSTSSTTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAA 300
           IRCRTT    AERHV KTYSTSSTTSCAAAPI PVLSCFS+ RR  STT+TTITEHAA +
Sbjct: 241 IRCRTT----AERHVTKTYSTSSTTSCAAAPIHPVLSCFSITRRPNSTTITTITEHAAES 300

Query: 301 AADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360
           AA  D   GEFDPEPEL+AEP+NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY
Sbjct: 301 AATVD---GEFDPEPELIAEPMNARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360

Query: 361 EMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKK 420
           EMLYGKTPFKGE+NEKTL+NILKQPL FPRIG S S+KEYEEMVKVEDLI+KLLVKNPKK
Sbjct: 361 EMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVS-SNKEYEEMVKVEDLIRKLLVKNPKK 420

Query: 421 RIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPSKYSSNYLPKLSKK 480
           RIGS KGSVEIKRHEFFKGVNWALIRSVRPPQ+PNSDHLLH  +  SKY SNY+PKL+KK
Sbjct: 421 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLLHKTLTQSKYPSNYVPKLTKK 480

Query: 481 QREQPYQIPPHHFDYF 496
           QR+QPYQIP HHFDYF
Sbjct: 481 QRQQPYQIPSHHFDYF 487

BLAST of Tan0014868 vs. NCBI nr
Match: XP_023536840.1 (protein kinase PINOID 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536841.1 protein kinase PINOID 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845.9 bits (2184), Expect = 1.8e-241
Identity = 425/496 (85.69%), Postives = 457/496 (92.14%), Query Frame = 0

Query: 1   MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVA 60
           MA+I RD+SD+DS+ SS+T+PDS+RSW+SNLS  S RRSS+S  DA  SP+KPHKAN VA
Sbjct: 1   MASITRDDSDHDSTSSSLTLPDSTRSWLSNLSISS-RRSSTSALDATLSPYKPHKANHVA 60

Query: 61  WEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREAL 120
           WEAMRW+R VKGRVGLEHFRLLRR+GSGDIGNVYLCEIRNPMVGLAQC+YAMKVVDREA+
Sbjct: 61  WEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLAQCYYAMKVVDREAV 120

Query: 121 SVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180
           SVRNK RRADMEKEILK+IDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR
Sbjct: 121 SVRNKGRRADMEKEILKLIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180

Query: 181 FSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRL 240
           F ISSAKFYAAEIIL LEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVP+L
Sbjct: 181 FPISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPKL 240

Query: 241 IRCRTTASDAAERHV-KTYSTSSTTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAA 300
           IRCRTT    +ERHV KTYSTSSTTSCAAAPI PVLSCFS+ RR  STT+TTITEHAA +
Sbjct: 241 IRCRTT----SERHVTKTYSTSSTTSCAAAPIHPVLSCFSITRRPNSTTITTITEHAAES 300

Query: 301 AADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360
           AA  D   GEFDPEPEL+AEP+NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY
Sbjct: 301 AATVD---GEFDPEPELIAEPMNARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360

Query: 361 EMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKK 420
           EMLYGKTPFKGE+NEKTL+NILKQPL FPRIG S S+KEYEEMVKVEDLI+KLLVKNPKK
Sbjct: 361 EMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVS-SNKEYEEMVKVEDLIRKLLVKNPKK 420

Query: 421 RIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPSKYSSNYLPKLSKK 480
           RIGS KGSVEIKRHEFFKGVNWALIRSVRPPQ+PNSDHLLH  +  SKY SNY+PKL+KK
Sbjct: 421 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLLHKTLTQSKYPSNYVPKLTKK 480

Query: 481 QREQPYQIPPHHFDYF 496
           QR+QPYQIP H+FDYF
Sbjct: 481 QRQQPYQIPSHYFDYF 487

BLAST of Tan0014868 vs. NCBI nr
Match: XP_022937480.1 (protein kinase PINOID 2-like [Cucurbita moschata])

HSP 1 Score: 844.3 bits (2180), Expect = 5.2e-241
Identity = 424/496 (85.48%), Postives = 456/496 (91.94%), Query Frame = 0

Query: 1   MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVA 60
           MA+I RD+SD+DS+ SS+T+ DS+RSW+SNLS  S RRSS+S  DA  SP+KPHKAN VA
Sbjct: 1   MASITRDDSDHDSTSSSLTLQDSTRSWLSNLSISS-RRSSTSALDAALSPYKPHKANHVA 60

Query: 61  WEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREAL 120
           WEAMRW+R VKGRVGLEHFRLLRR+GSGDIGNVYLCEIRNP+VGLAQC+YAMKVVDREA+
Sbjct: 61  WEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPVVGLAQCYYAMKVVDREAV 120

Query: 121 SVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180
           SVRNK RRADMEKEILK+IDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR
Sbjct: 121 SVRNKGRRADMEKEILKLIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180

Query: 181 FSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRL 240
           F ISS+KFYAAEIIL LEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVP+L
Sbjct: 181 FPISSSKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPKL 240

Query: 241 IRCRTTASDAAERHV-KTYSTSSTTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAA 300
           IRCRTT    AERHV KTYSTSSTTSCAAAPI PVLSCFS+ RR  STT+TTITEHAA +
Sbjct: 241 IRCRTT----AERHVTKTYSTSSTTSCAAAPIHPVLSCFSITRRPNSTTITTITEHAAES 300

Query: 301 AADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360
           AA  D   GEFDPEPEL+AEP+NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY
Sbjct: 301 AATVD---GEFDPEPELIAEPMNARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360

Query: 361 EMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKK 420
           EMLYGKTPFKGE+NEKTL+NILKQPL FPRIG S S+KEYEEMVKVEDLI+KLLVKNPKK
Sbjct: 361 EMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVS-SNKEYEEMVKVEDLIRKLLVKNPKK 420

Query: 421 RIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPSKYSSNYLPKLSKK 480
           RIGS KGSVEIKRHEFFKGVNWALIRSVRPPQ+PNSDHLLH  +  SKY SNY+PKL+KK
Sbjct: 421 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLLHKTLTQSKYPSNYVPKLTKK 480

Query: 481 QREQPYQIPPHHFDYF 496
           QR+QPYQIP HHFDYF
Sbjct: 481 QRQQPYQIPSHHFDYF 487

BLAST of Tan0014868 vs. NCBI nr
Match: XP_038889440.1 (LOW QUALITY PROTEIN: protein kinase PINOID 2 [Benincasa hispida])

HSP 1 Score: 827.8 bits (2137), Expect = 5.0e-236
Identity = 427/514 (83.07%), Postives = 457/514 (88.91%), Query Frame = 0

Query: 1   MAAIARDE-SDYDSSC-SSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSPH------- 60
           M +I +DE SDYDSSC SSITVPDS+RSWMSNLSFGSRRRSSSS AD     +       
Sbjct: 1   MGSINKDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGSIDNNIPTLPLQ 60

Query: 61  ---KPHKANQVAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQC 120
              KPHKAN+V WEAMRWLR VKG VGLEHFRLLRR+GSGDIG+VYLCEIRNPMVGL +C
Sbjct: 61  MMMKPHKANEVGWEAMRWLRRVKGEVGLEHFRLLRRVGSGDIGSVYLCEIRNPMVGLPKC 120

Query: 121 FYAMKVVDREALSVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGD 180
           FYAMKVVDREA+ VRNKERRADMEKEILKV+DHPFLPTLYA+FEASHYSCLVMEFCPGGD
Sbjct: 121 FYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYADFEASHYSCLVMEFCPGGD 180

Query: 181 LYAARQRLPGKRFSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDF 240
           LYAAR RLP KRFSISSAKFYAAEIIL LE +HMMGI+YRDLKPENVLVREDGHIMLSDF
Sbjct: 181 LYAARLRLPNKRFSISSAKFYAAEIILALEXMHMMGIIYRDLKPENVLVREDGHIMLSDF 240

Query: 241 DLCLKCDVVPRLIRCRTTASDAAERHVKTY-STSSTTSCAAAPIQPVLSCFSLARRRKST 300
           DLCLKCDVVP+LIRCRTTASD  +RHV++Y ST+STTS   APIQPVLSCFSL+RR+KST
Sbjct: 241 DLCLKCDVVPKLIRCRTTASD--DRHVRSYSSTTSTTSAPPAPIQPVLSCFSLSRRKKST 300

Query: 301 TVTTITEHAAAAA------ADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVIS 360
           TVTTITEHAAA A       D DGDG EFDPEPEL+AEPINARSKSFVGTHEYLAPEVIS
Sbjct: 301 TVTTITEHAAAPAVVYGGDGDSDGDGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVIS 360

Query: 361 GQGHGSAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEE 420
           GQGHGS VDWWTLGIFLYEMLYGKTPFKGE+NEKTL+NILKQPL FPRIG S SSKEYEE
Sbjct: 361 GQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGIS-SSKEYEE 420

Query: 421 MVKVEDLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHN 480
           MVKVEDLIKKLLVKNPKKRIGS KGSVEIKRHEFFKG+NWALIRSVRPPQIPN++   + 
Sbjct: 421 MVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHEFFKGINWALIRSVRPPQIPNNN---NP 480

Query: 481 NINPSKYSSNYLPKLSKKQREQPYQIPPHHFDYF 496
           N+N SKY SNYLPKLSKKQR+QP+QIP HHFDYF
Sbjct: 481 NLNYSKYPSNYLPKLSKKQRQQPFQIPNHHFDYF 508

BLAST of Tan0014868 vs. ExPASy TrEMBL
Match: A0A6J1HPF1 (protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465514 PE=4 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 3.7e-245
Identity = 430/496 (86.69%), Postives = 459/496 (92.54%), Query Frame = 0

Query: 1   MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVA 60
           MA I RD+SD+DS+CSS+T+PDSSRSWMSNLSFGS RRSS+S  DA  SP+KPHKANQVA
Sbjct: 1   MAGITRDDSDHDSTCSSLTLPDSSRSWMSNLSFGS-RRSSTSALDAALSPYKPHKANQVA 60

Query: 61  WEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREAL 120
           WEAMRW+R VKGRVGLEHFRLLRR+GSGDIGNVYLCEIRNP+VGLAQC+YAMKVVDREA+
Sbjct: 61  WEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPVVGLAQCYYAMKVVDREAV 120

Query: 121 SVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180
           SVRNK RRADMEKEILK+IDHPFLPTLYA+FEASHYSCLVMEFCPGGDLYAARQRLPGKR
Sbjct: 121 SVRNKGRRADMEKEILKLIDHPFLPTLYAQFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180

Query: 181 FSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRL 240
           F I S KFYAAEIIL LEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVP+L
Sbjct: 181 FPIPSVKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPKL 240

Query: 241 IRCRTTASDAAERHV-KTYSTSSTTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAA 300
           IRCRTT    AERHV KTYSTSSTTSCAAAPIQPVLSCFS+ RRR STT+TTITEHAA +
Sbjct: 241 IRCRTT----AERHVTKTYSTSSTTSCAAAPIQPVLSCFSITRRRNSTTITTITEHAAES 300

Query: 301 AADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360
           AA  D   GEFDPEPEL+AEP+NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY
Sbjct: 301 AATVD---GEFDPEPELIAEPMNARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360

Query: 361 EMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKK 420
           EMLYGKTPFKGE+NEKTL+NILKQPL FPRIG S S+KEYEEMVKVEDLI+KLLVKNPKK
Sbjct: 361 EMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVS-SNKEYEEMVKVEDLIRKLLVKNPKK 420

Query: 421 RIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPSKYSSNYLPKLSKK 480
           RIGS KGSVEIKRHEFFKGVNWALIRSVRPPQ+PNSDHLLH  +  SKY SNY+PKL+KK
Sbjct: 421 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLLHKTLTQSKYPSNYVPKLTKK 480

Query: 481 QREQPYQIPPHHFDYF 496
           QR+QPYQIP  HFDYF
Sbjct: 481 QRQQPYQIPSPHFDYF 487

BLAST of Tan0014868 vs. ExPASy TrEMBL
Match: A0A6J1FGS3 (protein kinase PINOID 2-like OS=Cucurbita moschata OX=3662 GN=LOC111443882 PE=4 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.5e-241
Identity = 424/496 (85.48%), Postives = 456/496 (91.94%), Query Frame = 0

Query: 1   MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVA 60
           MA+I RD+SD+DS+ SS+T+ DS+RSW+SNLS  S RRSS+S  DA  SP+KPHKAN VA
Sbjct: 1   MASITRDDSDHDSTSSSLTLQDSTRSWLSNLSISS-RRSSTSALDAALSPYKPHKANHVA 60

Query: 61  WEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREAL 120
           WEAMRW+R VKGRVGLEHFRLLRR+GSGDIGNVYLCEIRNP+VGLAQC+YAMKVVDREA+
Sbjct: 61  WEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPVVGLAQCYYAMKVVDREAV 120

Query: 121 SVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180
           SVRNK RRADMEKEILK+IDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR
Sbjct: 121 SVRNKGRRADMEKEILKLIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKR 180

Query: 181 FSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRL 240
           F ISS+KFYAAEIIL LEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVP+L
Sbjct: 181 FPISSSKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPKL 240

Query: 241 IRCRTTASDAAERHV-KTYSTSSTTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAA 300
           IRCRTT    AERHV KTYSTSSTTSCAAAPI PVLSCFS+ RR  STT+TTITEHAA +
Sbjct: 241 IRCRTT----AERHVTKTYSTSSTTSCAAAPIHPVLSCFSITRRPNSTTITTITEHAAES 300

Query: 301 AADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360
           AA  D   GEFDPEPEL+AEP+NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY
Sbjct: 301 AATVD---GEFDPEPELIAEPMNARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 360

Query: 361 EMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKK 420
           EMLYGKTPFKGE+NEKTL+NILKQPL FPRIG S S+KEYEEMVKVEDLI+KLLVKNPKK
Sbjct: 361 EMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVS-SNKEYEEMVKVEDLIRKLLVKNPKK 420

Query: 421 RIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPSKYSSNYLPKLSKK 480
           RIGS KGSVEIKRHEFFKGVNWALIRSVRPPQ+PNSDHLLH  +  SKY SNY+PKL+KK
Sbjct: 421 RIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLLHKTLTQSKYPSNYVPKLTKK 480

Query: 481 QREQPYQIPPHHFDYF 496
           QR+QPYQIP HHFDYF
Sbjct: 481 QRQQPYQIPSHHFDYF 487

BLAST of Tan0014868 vs. ExPASy TrEMBL
Match: A0A5D3BGM8 (Protein kinase PINOID 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00110 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.1e-232
Identity = 418/509 (82.12%), Postives = 452/509 (88.80%), Query Frame = 0

Query: 3   AIARDE-SDYDSSC-SSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSPH--------- 62
           +I RDE SDYDSSC SSITVPDS+RSWMSNLSFGSRRRSSSS AD G   +         
Sbjct: 4   SIGRDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGGLENITSGTTTTT 63

Query: 63  ---KPHKANQVAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQC 122
              KPHKAN+V WEAMRWLR  KG VGLE FRL+RR+GSGDIGNVYLCEIRNPMVGL + 
Sbjct: 64  MMVKPHKANEVGWEAMRWLRRAKGEVGLEDFRLIRRVGSGDIGNVYLCEIRNPMVGLPKW 123

Query: 123 FYAMKVVDREALSVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGD 182
           FYAMKVVDREA+ VRNKERRADMEKEILKV+DHPFLPTLYA FEASHYSCLVMEFCPGGD
Sbjct: 124 FYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLVMEFCPGGD 183

Query: 183 LYAARQRLPGKRFSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDF 242
           LYAAR RLP KRFSI SAKFYAAEIIL LEYLHMMGI+YRDLKPENVLVREDGHIMLSDF
Sbjct: 184 LYAARLRLPNKRFSIPSAKFYAAEIILALEYLHMMGIIYRDLKPENVLVREDGHIMLSDF 243

Query: 243 DLCLKCDVVPRLIRCRTTASDAAERHVK--TYSTSSTTSCAAAPIQPVLSCFSLARRRKS 302
           DLCLKCDVVP+L+R RTTASD   RH++  +Y+ S+++SCAAAPIQPVLSCFSL+RR+KS
Sbjct: 244 DLCLKCDVVPKLLRSRTTASD---RHLRSSSYTFSTSSSCAAAPIQPVLSCFSLSRRQKS 303

Query: 303 TTVTTITEHAAAAAADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHG 362
           TTVTTITEHA+ +      DG EFDPEPEL+AEPINARSKSFVGTHEYLAPEVISGQGHG
Sbjct: 304 TTVTTITEHASDSIYGPGDDGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQGHG 363

Query: 363 SAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVE 422
           S VDWWTLGIFLYEMLYGKTPFKGE+NEKTL+NILKQPL FPRIG S SSKEYEEMVKVE
Sbjct: 364 SGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLKFPRIGIS-SSKEYEEMVKVE 423

Query: 423 DLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPS 482
           DLIKKLLVKNPKKRIGS KGSVEIKRHEFFKG+NWALIRSVRPPQIPN +++++NN+N S
Sbjct: 424 DLIKKLLVKNPKKRIGSLKGSVEIKRHEFFKGINWALIRSVRPPQIPN-NNIINNNLNYS 483

Query: 483 KYSSNYLPKLSKKQREQPYQIPPHHFDYF 496
           KY SNYLPKLSKKQR+QPYQIP HHFDYF
Sbjct: 484 KYPSNYLPKLSKKQRQQPYQIPNHHFDYF 507

BLAST of Tan0014868 vs. ExPASy TrEMBL
Match: A0A1S3C6E4 (protein kinase PINOID 2 OS=Cucumis melo OX=3656 GN=LOC103497373 PE=4 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 1.4e-231
Identity = 417/509 (81.93%), Postives = 452/509 (88.80%), Query Frame = 0

Query: 3   AIARDE-SDYDSSC-SSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSPH--------- 62
           +I RDE SDYDSSC SSITVPDS+RSWMSNLSFGSRRRSSSS AD G   +         
Sbjct: 4   SIGRDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGGLENITSGTTTTT 63

Query: 63  ---KPHKANQVAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQC 122
              KPHKAN+V WEAMRWLR  KG VGLE FRL+RR+GSGDIGNVYLCEIRNPMVGL + 
Sbjct: 64  MMVKPHKANEVGWEAMRWLRRAKGEVGLEDFRLIRRVGSGDIGNVYLCEIRNPMVGLPKW 123

Query: 123 FYAMKVVDREALSVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGD 182
           FYAMKVVDREA+ VRNKERRADMEKEILKV+DHPFLPTLYA FEASHYSCLVMEFCPGGD
Sbjct: 124 FYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLVMEFCPGGD 183

Query: 183 LYAARQRLPGKRFSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDF 242
           LYAAR RLP KRFSI SAKFYAAEIIL LEYLHMMGI+YRDLKPENVLVREDGHIMLSDF
Sbjct: 184 LYAARLRLPNKRFSIPSAKFYAAEIILALEYLHMMGIIYRDLKPENVLVREDGHIMLSDF 243

Query: 243 DLCLKCDVVPRLIRCRTTASDAAERHVK--TYSTSSTTSCAAAPIQPVLSCFSLARRRKS 302
           DLCLKCDVVP+L+R RTTASD   RH++  +Y+ S+++SCAAAPIQPVLSCFSL+RR+KS
Sbjct: 244 DLCLKCDVVPKLLRSRTTASD---RHLRSSSYTFSTSSSCAAAPIQPVLSCFSLSRRQKS 303

Query: 303 TTVTTITEHAAAAAADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHG 362
           TTVTTITE+A+ +      DG EFDPEPEL+AEPINARSKSFVGTHEYLAPEVISGQGHG
Sbjct: 304 TTVTTITENASDSIYGPGDDGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQGHG 363

Query: 363 SAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVE 422
           S VDWWTLGIFLYEMLYGKTPFKGE+NEKTL+NILKQPL FPRIG S SSKEYEEMVKVE
Sbjct: 364 SGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLKFPRIGIS-SSKEYEEMVKVE 423

Query: 423 DLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPS 482
           DLIKKLLVKNPKKRIGS KGSVEIKRHEFFKG+NWALIRSVRPPQIPN +++++NN+N S
Sbjct: 424 DLIKKLLVKNPKKRIGSLKGSVEIKRHEFFKGINWALIRSVRPPQIPN-NNIINNNLNYS 483

Query: 483 KYSSNYLPKLSKKQREQPYQIPPHHFDYF 496
           KY SNYLPKLSKKQR+QPYQIP HHFDYF
Sbjct: 484 KYPSNYLPKLSKKQRQQPYQIPNHHFDYF 507

BLAST of Tan0014868 vs. ExPASy TrEMBL
Match: A0A6J1KGU7 (protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493762 PE=4 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 8.9e-231
Identity = 418/498 (83.94%), Postives = 443/498 (88.96%), Query Frame = 0

Query: 1   MAAIARDESDYDSSCSSITVPDSSRSWMSNLSFGSRRRSSSSGADAGSSP-HKPHKANQV 60
           MAAIA+DESDYDSSCSSIT+PDS+RSWMSNLSFGS+RR SSS A  G+SP  KPHKAN+V
Sbjct: 1   MAAIAKDESDYDSSCSSITLPDSTRSWMSNLSFGSQRRRSSSSATDGTSPVQKPHKANEV 60

Query: 61  AWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIR--NPMVGLAQCFYAMKVVDR 120
           AWEAMRWLRG KG VGLE+FRLLRRLGSGDIGNVYLC+IR   P   + QC YAMKVVDR
Sbjct: 61  AWEAMRWLRGAKGEVGLENFRLLRRLGSGDIGNVYLCQIRKPTPTARVGQCCYAMKVVDR 120

Query: 121 EALSVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLP 180
           EA+ VRNKERRA+MEKEILK+IDHPFLPTLYA FEASHYSCLVMEFCPGGDLYAAR R P
Sbjct: 121 EAVCVRNKERRAEMEKEILKMIDHPFLPTLYAAFEASHYSCLVMEFCPGGDLYAARLRQP 180

Query: 181 GKRFSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVV 240
            KRFSISSAKFYAAEIIL LEYLHMMGIVYRDLKPENVLVR DGHIMLSDFDLCLKCDVV
Sbjct: 181 AKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVRHDGHIMLSDFDLCLKCDVV 240

Query: 241 PRLIRCRTTASDAAERHVKTYSTSSTTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAA 300
           P+LIR RTTASD  ER+V++YSTSSTTSCAA PI+PVLSCFS+ARRRK TTVTTITEH  
Sbjct: 241 PKLIRSRTTASDVTERYVRSYSTSSTTSCAAVPIKPVLSCFSIARRRKPTTVTTITEH-- 300

Query: 301 AAAADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIF 360
              ADDDG   EFD EPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWT+GIF
Sbjct: 301 ---ADDDGYREEFDTEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWTVGIF 360

Query: 361 LYEMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNP 420
           LYEMLYGKTPFKGE+NEKTL+NIL+QPL FPRIGFS SSKEYEEMVKVEDLIKKLLVKNP
Sbjct: 361 LYEMLYGKTPFKGENNEKTLMNILRQPLTFPRIGFS-SSKEYEEMVKVEDLIKKLLVKNP 420

Query: 421 KKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIPNSDHLLHNNINPSKYSSNYLPKLS 480
           KKRIGS KGS+EIKRHEFFKGVNWALIRSVRPP IPNSDHL     NP   S N  PKLS
Sbjct: 421 KKRIGSLKGSIEIKRHEFFKGVNWALIRSVRPPHIPNSDHL-----NPR--SKN--PKLS 480

Query: 481 KKQREQPYQIPPHHFDYF 496
           K+QR+QPYQIP   FDYF
Sbjct: 481 KQQRQQPYQIPSDRFDYF 483

BLAST of Tan0014868 vs. TAIR 10
Match: AT2G26700.1 (AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein )

HSP 1 Score: 543.9 bits (1400), Expect = 1.3e-154
Identity = 305/528 (57.77%), Postives = 367/528 (69.51%), Query Frame = 0

Query: 7   DESDYDSSCSSITVPDSSR--SWMSNLSFGSRRRSSSSG-ADAGSSPH---KPHKANQVA 66
           +ESDY+SS      PDSSR  SW+S+    S   SS S  ++ G + +   KPHKANQVA
Sbjct: 11  NESDYESSTVG---PDSSRRTSWLSSSFTASPSCSSISHLSNHGLNSYNQSKPHKANQVA 70

Query: 67  WEAMRWLRGVKGR-VGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREA 126
           WEAM  LR   GR VGLEHFRLL+RLGSGDIG+VYLC+IR         FYAMKVVD+EA
Sbjct: 71  WEAMARLRRCCGRAVGLEHFRLLKRLGSGDIGSVYLCQIRG---SPETAFYAMKVVDKEA 130

Query: 127 LSVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGK 186
           ++V+ K  RA+MEK+IL ++DHPF PTLYA FEASHYS LVME+CPGGDLYA R R P K
Sbjct: 131 VAVKKKLGRAEMEKKILGMLDHPFCPTLYAAFEASHYSFLVMEYCPGGDLYAVRLRQPSK 190

Query: 187 RFSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPR 246
           RF+ISS +FYAAE ++ LEYLHMMGIVYRDLKPENVL+REDGH+MLSDFDL  KCDVVP+
Sbjct: 191 RFTISSTRFYAAETLVALEYLHMMGIVYRDLKPENVLIREDGHVMLSDFDLSFKCDVVPQ 250

Query: 247 LI--RCRTTASDAAERHVKTYSTSSTTSCAAAPIQPVLSCFS--LARRRKSTTVTTITEH 306
            +    R       +  +      ST SC   P+ PV+SCFS   +RRRK   VTT    
Sbjct: 251 FLSDNDRDRGHQEDDDDISIRRKCSTPSCTTTPLNPVISCFSPTSSRRRKKNVVTTTIHE 310

Query: 307 AAAAAADD-------------------------DGDGGEFDPEPELVAEPINARSKSFVG 366
            AA  +D                          D  GG     P + AEPINARSKSFVG
Sbjct: 311 NAAGTSDSVKSNDVSRTFSRSPSSCSRVSNGLRDISGG----CPSIFAEPINARSKSFVG 370

Query: 367 THEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINILKQPLAFPRI 426
           THEYLAPEVISGQGHGSAVDWWT GIFLYEM++G+TPFKG++NEKTL+NILK PL FP++
Sbjct: 371 THEYLAPEVISGQGHGSAVDWWTYGIFLYEMIFGRTPFKGDNNEKTLVNILKAPLTFPKV 430

Query: 427 GFSNSSKEYEEMVKVEDLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPP 486
              NS KEYE+MV  +DLI KLLVKNPKKR+GS KGS+EIKRHEFF+GVNWALIRS++PP
Sbjct: 431 -IVNSPKEYEDMVNAQDLIIKLLVKNPKKRLGSLKGSIEIKRHEFFEGVNWALIRSIKPP 490

Query: 487 QIPNSDHLLHNNINPSKYSSNYLP---KLSKKQREQPYQIPPHHFDYF 496
            +P  +   H     ++  + YLP    +S+K+R +PY +  ++FDYF
Sbjct: 491 WVP-KEETSHKTKGDNRSVNYYLPPRFMMSRKERNEPYHV-SNYFDYF 525

BLAST of Tan0014868 vs. TAIR 10
Match: AT4G26610.1 (D6 protein kinase like 1 )

HSP 1 Score: 427.2 bits (1097), Expect = 1.8e-119
Identity = 216/417 (51.80%), Postives = 277/417 (66.43%), Query Frame = 0

Query: 39  SSSSGADAGSSPHKPHKANQVAWEAMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEI 98
           S  S   + S  +KPHKAN V WEA++ +R   G +GL HFRLL+RLG GDIG V+L E+
Sbjct: 83  SDESTCSSFSGNNKPHKANDVRWEAIQAVRTKHGVLGLNHFRLLKRLGCGDIGTVHLAEL 142

Query: 99  RNPMVGLAQCFYAMKVVDREALSVRNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSC 158
                   +CF+AMKV+D+ AL+ R K  RA  E+EIL+ +DHPFLPTLY+ FE   +SC
Sbjct: 143 HG-----TRCFFAMKVMDKGALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSC 202

Query: 159 LVMEFCPGGDLYAARQRLPGKRFSISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVR 218
           LVMEFCPGGDL+  RQR PGKRFS  +AKFY AE++L +EYLHM+GI+YRDLKPENVLVR
Sbjct: 203 LVMEFCPGGDLHTLRQRQPGKRFSEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVR 262

Query: 219 EDGHIMLSDFDLCLKCDVVPRLIRCRTTASDAAER--HVKTYSTSSTTSCAAAPIQPVLS 278
           +DGH+MLSDFDL L+C V P ++R    AS+  +   +    +     SC +AP      
Sbjct: 263 DDGHVMLSDFDLSLRCTVSPTVVRSTVLASEGQKNSGYCAQPACIQQPSCISAPTTCFSP 322

Query: 279 CFSLARRRKSTTVTTITEHAAAAAADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLA 338
            +  ++ +K   +   T             G +  P PELVAEP +ARS SFVGTHEYLA
Sbjct: 323 RYFSSKSKKDKKMKNET-------------GNQVSPLPELVAEPTSARSMSFVGTHEYLA 382

Query: 339 PEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSS 398
           PE+I G+GHGSAVDWWT GIFLYE+L+GKTPFKG  N  TL N++ QPL FP     + +
Sbjct: 383 PEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPESPVVSFA 442

Query: 399 KEYEEMVKVEDLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIP 454
                     DLI+ LLVK P+ R+   +G+ E+K+H FF+GVNWAL+R   PP+IP
Sbjct: 443 --------ARDLIRSLLVKEPQHRLAYKRGATEMKQHPFFEGVNWALVRCASPPEIP 473

BLAST of Tan0014868 vs. TAIR 10
Match: AT5G55910.1 (D6 protein kinase )

HSP 1 Score: 419.1 bits (1076), Expect = 5.0e-117
Identity = 221/445 (49.66%), Postives = 285/445 (64.04%), Query Frame = 0

Query: 22  DSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVAWEAMRWLRGVKGRVGLEHFRL 81
           +S +S     S  S     S+ +   SS +KPHKAN V WEA++ +R   G +GL HFRL
Sbjct: 52  ESGKSSTCRPSTSSDISDESTCSSFSSSINKPHKANDVRWEAIQAVRTKHGGLGLNHFRL 111

Query: 82  LRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREALSVRNKERRADMEKEILKVIDH 141
           L+RLG GDIG V+L E+        +C++AMKV+D+ AL+ R K  RA  E+EIL+ +DH
Sbjct: 112 LKRLGCGDIGTVHLAELNG-----TRCYFAMKVMDKTALASRKKLLRAQTEREILQCLDH 171

Query: 142 PFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKRFSISSAKFYAAEIILGLEYLH 201
           PFLPTLY+ FE   +SCLVMEFCPGGDL+  RQR PGKRF+  +AKFY AE++L +EYLH
Sbjct: 172 PFLPTLYSHFETEKFSCLVMEFCPGGDLHTLRQRQPGKRFTEQAAKFYVAEVLLAMEYLH 231

Query: 202 MMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRLIRCRTTASDAAERHVKTYSTS 261
           M+GI+YRDLKPENVLVR+DGH+MLSDFDL L+C V   ++R     S+   ++  + S S
Sbjct: 232 MLGIIYRDLKPENVLVRDDGHVMLSDFDLSLRCTVSLSIVRSANVGSEGLSKN--SVSCS 291

Query: 262 STTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAAAADDDGDGGEFDPEPELVAEPI 321
              +C   P     SC S+A           ++         +    +  P PELVAEP 
Sbjct: 292 QQPACIQQP-----SCISMAPTSCFGPRFFSSKSKKDKKPKTENGNHQVTPLPELVAEPT 351

Query: 322 NARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINIL 381
            ARS SFVGTHEYLAPE+I G+GHGSAVDWWT GIFLYE+L+GKTPFKG  N  TL N++
Sbjct: 352 GARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVV 411

Query: 382 KQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVNW 441
            QPL FP     + +          DLI+ LLVK P+ R+   +G+ EIK+H FF+GVNW
Sbjct: 412 GQPLRFPESPVVSFA--------ARDLIRSLLVKEPQHRLAYKRGATEIKQHPFFEGVNW 471

Query: 442 ALIRSVRPPQIPNSDHLLHNNINPS 467
           AL+R   PP+IP    L   N  P+
Sbjct: 472 ALVRCASPPEIPKPVDLEALNPTPT 476

BLAST of Tan0014868 vs. TAIR 10
Match: AT5G47750.1 (D6 protein kinase like 2 )

HSP 1 Score: 417.2 bits (1071), Expect = 1.9e-116
Identity = 219/433 (50.58%), Postives = 281/433 (64.90%), Query Frame = 0

Query: 22  DSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVAWEAMRWLRGVKGRVGLEHFRL 81
           +S +S +   S  S     SS +   S+ +KPHKAN + WEA++ +R   G +GL HFRL
Sbjct: 134 ESGKSSICRGSTSSDVSDESSCSSFSSTVNKPHKANDLRWEAIQAVRVRDGLLGLSHFRL 193

Query: 82  LRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREALSVRNKERRADMEKEILKVIDH 141
           L+RLG GDIG+VYL E+        +C++AMKV+D+ +L+ R K  RA  E+EIL+ +DH
Sbjct: 194 LKRLGCGDIGSVYLSELSG-----TKCYFAMKVMDKTSLASRKKLLRAQTEREILQSLDH 253

Query: 142 PFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKRFSISSAKFYAAEIILGLEYLH 201
           PFLPTLY  FE   +SCLVMEFCPGGDL+  RQR PGK FS  + KFY AE +L LEYLH
Sbjct: 254 PFLPTLYTHFETEKFSCLVMEFCPGGDLHTLRQRQPGKHFSEQAVKFYIAESLLALEYLH 313

Query: 202 MMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRLIRCRTTASDAAERHVKTYSTS 261
           M+GIVYRDLKPENVLVREDGHIMLSDFDL L+C V P L++     SD   ++V     +
Sbjct: 314 MLGIVYRDLKPENVLVREDGHIMLSDFDLSLRCLVSPTLVKSAAIESDPLRKNVYCVQPA 373

Query: 262 S-TTSCAAAPIQPVLSCFSLARRRKSTTVTTITEHAAAAAADDDGDGGEFDPEPELVAEP 321
               SC         +CFS             +  +       +    +  P PELVAEP
Sbjct: 374 CIEPSCIQPSCTVPTTCFS---------PRLFSSKSKKDRKPKNDTANQVRPLPELVAEP 433

Query: 322 INARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGESNEKTLINI 381
            +ARS SFVGTHEYLAPE+I G+GHGSAVDWWT GIFLYE+L+G+TPFKG  N +TL N+
Sbjct: 434 TDARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGRTPFKGSGNRQTLFNV 493

Query: 382 LKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLLVKNPKKRIGSFKGSVEIKRHEFFKGVN 441
           + QPL FP     + +          DLI+ LL+K P++R+G  +G+ E+K+H FF+GVN
Sbjct: 494 VGQPLRFPETPVVSFA--------ARDLIRGLLMKEPQQRLGFKRGATEVKQHPFFEGVN 544

Query: 442 WALIRSVRPPQIP 454
           WALIR   PP+IP
Sbjct: 554 WALIRCATPPEIP 544

BLAST of Tan0014868 vs. TAIR 10
Match: AT2G44830.1 (Protein kinase superfamily protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.8e-112
Identity = 221/460 (48.04%), Postives = 293/460 (63.70%), Query Frame = 0

Query: 4   IARDESDYDSSCSSITVP-DSSRSWMSNLSFGSRRRSSSSGADAGSSPHKPHKANQVAWE 63
           ++ D++ +D S S +    +S+++  S  S  S     SS ++   S +KPHK N   W 
Sbjct: 287 LSMDKNYFDRSISMVLDSFESTKTSASRASDSSGLSEESSWSNFTGSLNKPHKGNDPWWN 346

Query: 64  AMRWLRGVKGRVGLEHFRLLRRLGSGDIGNVYLCEIRNPMVGLAQCFYAMKVVDREALSV 123
           A+  +R   G +G+ HF+LL+RLG GDIG+VYL E+        +C +A+KV+D+ +L  
Sbjct: 347 AILAIRTRDGILGMSHFKLLKRLGCGDIGSVYLAELSG-----TRCHFAVKVMDKASLED 406

Query: 124 RNKERRADMEKEILKVIDHPFLPTLYAEFEASHYSCLVMEFCPGGDLYAARQRLPGKRFS 183
           R K  RA  E++IL+++DHPFLPTLY  FE   +SCLVME+CPGGDL+  RQR PGK FS
Sbjct: 407 RKKLNRAQTERDILQLLDHPFLPTLYTHFETDRFSCLVMEYCPGGDLHTLRQRQPGKHFS 466

Query: 184 ISSAKFYAAEIILGLEYLHMMGIVYRDLKPENVLVREDGHIMLSDFDLCLKCDVVPRLIR 243
             +A+FYAAE++L LEYLHM+G+VYRDLKPENVLVR+DGHIMLSDFDL L+C V P LI 
Sbjct: 467 EYAARFYAAEVLLALEYLHMLGVVYRDLKPENVLVRDDGHIMLSDFDLSLRCAVSPTLI- 526

Query: 244 CRTTASDAAERHVKTYSTSSTTSCAAAPIQPVLSCF---------SLARRRKSTTVTTIT 303
            +T  SD + R       +     +A  IQP  SCF         +   + + T      
Sbjct: 527 -KTFDSDPSRRGAFCVQPACMEPTSACIIQP--SCFLPRSIFPNKNKKNKSRKTQADFFK 586

Query: 304 EHAAAAAADDDGDGGEFDPEPELVAEPINARSKSFVGTHEYLAPEVISGQGHGSAVDWWT 363
            H+ +               PELVAEP N RS SFVGTHEYLAPE+I G+GHGSAVDWWT
Sbjct: 587 SHSGSL--------------PELVAEP-NTRSMSFVGTHEYLAPEIIKGEGHGSAVDWWT 646

Query: 364 LGIFLYEMLYGKTPFKGESNEKTLINILKQPLAFPRIGFSNSSKEYEEMVKVEDLIKKLL 423
            GIF++E+LYGKTPFKG  N  TL N++ + L FP     + +  Y       DLI+ LL
Sbjct: 647 FGIFVHELLYGKTPFKGSGNRATLFNVVGEQLKFP----ESPATSYAG----RDLIQALL 706

Query: 424 VKNPKKRIGSFKGSVEIKRHEFFKGVNWALIRSVRPPQIP 454
           VK+PK R+G+ +G+ EIK+H FF+GVNWALIR   PP++P
Sbjct: 707 VKDPKNRLGTKRGATEIKQHPFFEGVNWALIRCSTPPEVP 714

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94E499.1e-16459.41Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV... [more]
Q64FQ21.9e-15357.77Protein kinase PINOID 2 OS=Arabidopsis thaliana OX=3702 GN=PID2 PE=1 SV=1[more]
Q9SUA32.6e-11851.80Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1... [more]
Q9FG747.0e-11649.66Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=... [more]
Q391832.7e-11550.58Serine/threonine-protein kinase D6PKL2 OS=Arabidopsis thaliana OX=3702 GN=D6PKL2... [more]
Match NameE-valueIdentityDescription
XP_022965690.17.7e-24586.69protein kinase PINOID 2-like [Cucurbita maxima][more]
KAG7021258.12.1e-24285.89Protein kinase PINOID 2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023536840.11.8e-24185.69protein kinase PINOID 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_0235368... [more]
XP_022937480.15.2e-24185.48protein kinase PINOID 2-like [Cucurbita moschata][more]
XP_038889440.15.0e-23683.07LOW QUALITY PROTEIN: protein kinase PINOID 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1HPF13.7e-24586.69protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465514 PE=4 SV... [more]
A0A6J1FGS32.5e-24185.48protein kinase PINOID 2-like OS=Cucurbita moschata OX=3662 GN=LOC111443882 PE=4 ... [more]
A0A5D3BGM82.1e-23282.12Protein kinase PINOID 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3C6E41.4e-23181.93protein kinase PINOID 2 OS=Cucumis melo OX=3656 GN=LOC103497373 PE=4 SV=1[more]
A0A6J1KGU78.9e-23183.94protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493762 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT2G26700.11.3e-15457.77AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein [more]
AT4G26610.11.8e-11951.80D6 protein kinase like 1 [more]
AT5G55910.15.0e-11749.66D6 protein kinase [more]
AT5G47750.11.9e-11650.58D6 protein kinase like 2 [more]
AT2G44830.12.8e-11248.04Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 79..436
e-value: 5.6E-79
score: 278.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 325..436
e-value: 3.8E-22
score: 78.9
coord: 80..233
e-value: 5.3E-32
score: 111.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 79..436
score: 43.156063
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 77..163
e-value: 2.0E-46
score: 160.4
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 164..233
e-value: 2.0E-46
score: 160.4
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 323..454
e-value: 1.2E-37
score: 131.5
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 330..426
e-value: 74.0
score: 0.2
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 75..231
e-value: 1.1E-12
score: 45.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..47
NoneNo IPR availablePANTHERPTHR45637:SF12PROTEIN KINASE PINOID 2coord: 1..495
NoneNo IPR availablePANTHERPTHR45637FLIPPASE KINASE 1-RELATEDcoord: 1..495
NoneNo IPR availableCDDcd05574STKc_phototropin_likecoord: 78..453
e-value: 1.74312E-163
score: 463.247
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 205..217
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 56..458

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0014868.1Tan0014868.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity