Tan0014744 (gene) Snake gourd v1

Overview
NameTan0014744
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationLG01: 99166292 .. 99169577 (-)
RNA-Seq ExpressionTan0014744
SyntenyTan0014744
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAGCACAACTGAAAGCTTTGTGTTTTTAGCTTTGATATGTTTGTTTTCATCTCTTTCTTATAATTCTTTACCTCTTTCGACGAGAGGGAGATGGATCGTGGACTCGACCACGGGGCGTCGAGTGAAGCTTATGTGCGTGAATTGGCCTTCCCACACGCAGAGCATGCTAATCGAAGGCCTCGACCGCAGGGCGTTGAAAGACCTTGCCGACGAGGCAATGAGGTTGAGGTTCAATTGTGTAAGGCTCACATATGCGACTCACATGTTTACTCGCTATGCTAATAGGACGGTTGAAGAGAATTTTGACCTTCTTGATTTGAAAGCTTCTAAGGCTGGATTGGCTTTGCATAATCCATTTGTCTTGAACATGACCATATTTGAGGCCTTTGAAGCTGCGGTTGATGTGCTTGGGATGGCTGGTTTAATGGTGATAGCTGACAATCATATTACCCAACCAACATGGTGTTGCTCTCTTCACGATGGAAATGGTTTCTTTGGAGATCGCTATTTCGACCCCCAAGAATGGTTGGAAGCTCTTAGTTTGGTTGCTTCGCGGTTTTGCAACAAATCAACTGTGAGTAAATCTTCGACTTTTGAAATGATTTTTATACAAGTAATGGTAAGTATAGTCCATTTGTAAACACAAATAATAATGCATAATCTCAAGTAATTATTCGTTTTTTAATAAAACAAATATCGGTATGAGGATTTGAACATATTAATTATAATTGAGTTATGCTCACTTTAGTTAGTAATAATTATTGATTTACCTAGGCATCGATTGATATAGATATTTATTAAAATAACCAAAGTAATCAAGTATCTTATGGTAGCTTTTTTTAGTTATCAATTCTTTAATAATGTAGAATTTTATTATGGTTGTTAGCTTACAATTTTAATCATTTTATACTTCTAAATCCTGATAATTTCAATTTTTGTCTCTGATATTTACTAAAATTTCTAGAGCAATTCTATGTTGGGTGACCTCCTGAGAATTTTCCTAGGGAGCATGTGAGTGAGGACAAAACATGTTGAAAACGACCCTGTGTTGGTTTGTGGGATAGTGTTACAAGCAGGAAGATATAGTGACGCTCCTAGGCCTGGGACGTTATATAGGAGTTCTATTATTTCTAACTAATTGATAATTAAATATGTCATATGAGTTTTAATCAATTAGTAAGCATGTTAAACCTTTCAAAAAAAAAAAGAAGCATGTTAAAACCAATACCAAGAAACCCAATTGAAGTCAATGATAAAATATGAAACTCACATGATACATTCATTACTTCATAAGGAAGATCTTATTTTAAAATTTTGTCTCTCCTTAAGAAAATTTCTAACTCCACCTTTACGTCAACACAGGTGGTAGCCATGAGTCTACGAAATGAGCTCCGAGGAACAAAATCGAACTCAAAAGATTGGAATACATACATAACACAAGGAGCAACAACTATCCACAACATAAATCCAAACATCCTCGTAATCGTTTCGGGTCTAAACTTCGACAACGACCTAAGATGCCAAAGGCAAAATCCCTTGGCACTAAACAACAACCTACACAAGAAGCTAGTTTTTGAAGTGCACTTGTATTCTTTCAGTGGAGACTCCCAAACAAAGTTCATAAAAAATCCACTCAACAAAATATGCTCAAAGATCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGCGAGTGAGTTTGGGTTTGACCAAAGGGGAGTGAATGAGGCTGATAATAGGTTTTTGAGTTGCTTTCTTGCTCATCTTGCTAAGATGGATTTTGATTGGGCGCTATGGTCTTGGCAAGGGAGTTATTATTATCGAGAGGGTCACGCTCGATCGGATGAAGTGTTTGGAGTCTTGAATTACAATTGGAGTGATGTTAGAAACCCTCGTTTTTCTCAGATGTTTCAGCTATTGCAAACCATGTTGCAAGGTATGGTTTTGTTATATTAACTAGTACAATCTTATTTGATAACGATCTTGTTTTAGTTTTTTTTTTTTCAAATTTAATTTATGAAGATGTTTGAACTTTTTAAAAAAATTGATGAACTTGAAACGTTTAATTAAGACAAAATGACCAAATTGAACCAAAAAAAAAAAAACCAAAATATTAGCCTTTCTATGAAAAACTACTCCTTTTTCTTTAATAATATATTTAAATACTAAAGATACTATACTCTATAAGTTTAATTTAACCTGATATCCAAGAAAAATATTTTGGATTCGAATTCTCCTAAAGTTATTTCTTCCCTCAATTTATATTAATAGTTTTTTGCCAAAACAATAACAACATTAATAGTCTACACATTTTGTGAATCACATTAATATTAGGAAAGAGTGTATAAGAATATATGTAAAGTTTAAATATACGATAATGGAAAATTTTAGGTCCGACTATTTTATTTTTGGTTTTTTGTTTTTGAAAGTTATGTTGGTTTCCTCACAACTTTGAAGTAAGTTTTTCACCTTCGTCTTGAACAAACTTCAAAAACAAAACCGAGTTTTTAAAACTTCTCAAGACTTAGCTTGAATTTTGAAAATATTTTTAAAAAGTAGATCATAAAACAAAAAAAAAAATAGTATAGTAAGTTTAATTTTAAAAAATACAAAACCTAAAACAAAATTGTTATCAAAGTGTCTAAAAATTAGGTTAAAGTATTGATGGTAACTATTATGTTTAAAATTTTCTGTTTTTGACTAATCAACTATCAAGCTTTAAAAAAAAATTGAAAAGTACTACATATATATAAATATATAGTTCAATATTTTGTTATATTTTTGGTAATTACTGATCTATGCATGCAGATCCTAATTCCAATTCCCCAAACTCTTATGTAATGTATCATCCACAAAGTGGACAATGTGTCCAAGTGAAAGACATGATGAGTAAGGAAATTTATCTGAACAACTGTTCTAAGGCAAGCCATTGGAGTCATGATGGAGATGGGACACCAATCATGTTGGAGGGCACTGGTTTGTGCCTAAAAGCCATTGGAAATGGGCTTCAACCCTTACTTTCAAGGGACTGTTTGGGTGAGCAAAGTGCTTGGAAAGCCATTTCAAACTCTAAGCTTCATTTGGCCACACTCACAAAACAAGGGAATATTGGTTTGTGTTTAGAGAAATTAGAGAGCTCAAATTCAACTAGGGTTGTGATGGGCAACTGTGTTTGTGTTGGTGATGATTCAAATTGCTTACAAGATACTCAACCCCAATGGTTTGAACTTGTTCTCACAAATACTTTGTAA

mRNA sequence

ATGGGAAGCACAACTGAAAGCTTTGTGTTTTTAGCTTTGATATGTTTGTTTTCATCTCTTTCTTATAATTCTTTACCTCTTTCGACGAGAGGGAGATGGATCGTGGACTCGACCACGGGGCGTCGAGTGAAGCTTATGTGCGTGAATTGGCCTTCCCACACGCAGAGCATGCTAATCGAAGGCCTCGACCGCAGGGCGTTGAAAGACCTTGCCGACGAGGCAATGAGGTTGAGGTTCAATTGTGTAAGGCTCACATATGCGACTCACATGTTTACTCGCTATGCTAATAGGACGGTTGAAGAGAATTTTGACCTTCTTGATTTGAAAGCTTCTAAGGCTGGATTGGCCTTTGAAGCTGCGGTTGATGTGCTTGGGATGGCTGGTTTAATGGTGATAGCTGACAATCATATTACCCAACCAACATGGTGTTGCTCTCTTCACGATGGAAATGGTTTCTTTGGAGATCGCTATTTCGACCCCCAAGAATGGTTGGAAGCTCTTAGTTTGGTTGCTTCGCGGTTTTGCAACAAATCAACTGTGGTAGCCATGAGTCTACGAAATGAGCTCCGAGGAACAAAATCGAACTCAAAAGATTGGAATACATACATAACACAAGGAGCAACAACTATCCACAACATAAATCCAAACATCCTCGTAATCGTTTCGGGTCTAAACTTCGACAACGACCTAAGATGCCAAAGGCAAAATCCCTTGGCACTAAACAACAACCTACACAAGAAGCTAGTTTTTGAAGTGCACTTGTATTCTTTCAGTGGAGACTCCCAAACAAAGTTCATAAAAAATCCACTCAACAAAATATGCTCAAAGATCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGCGAGTGAGTTTGGGTTTGACCAAAGGGGAGTGAATGAGGCTGATAATAGGTTTTTGAGTTGCTTTCTTGCTCATCTTGCTAAGATGGATTTTGATTGGGCGCTATGGTCTTGGCAAGGGAGTTATTATTATCGAGAGGGTCACGCTCGATCGGATGAAGTGTTTGGAGTCTTGAATTACAATTGGAGTGATGTTAGAAACCCTCGTTTTTCTCAGATGTTTCAGCTATTGCAAACCATGTTGCAAGATCCTAATTCCAATTCCCCAAACTCTTATGTAATGTATCATCCACAAAGTGGACAATGTGTCCAAGTGAAAGACATGATGAGTAAGGAAATTTATCTGAACAACTGTTCTAAGGCAAGCCATTGGAGTCATGATGGAGATGGGACACCAATCATGTTGGAGGGCACTGGTTTGTGCCTAAAAGCCATTGGAAATGGGCTTCAACCCTTACTTTCAAGGGACTGTTTGGGTGAGCAAAGTGCTTGGAAAGCCATTTCAAACTCTAAGCTTCATTTGGCCACACTCACAAAACAAGGGAATATTGGTTTGTGTTTAGAGAAATTAGAGAGCTCAAATTCAACTAGGGTTGTGATGGGCAACTGTGTTTGTGTTGGTGATGATTCAAATTGCTTACAAGATACTCAACCCCAATGGTTTGAACTTGTTCTCACAAATACTTTGTAA

Coding sequence (CDS)

ATGGGAAGCACAACTGAAAGCTTTGTGTTTTTAGCTTTGATATGTTTGTTTTCATCTCTTTCTTATAATTCTTTACCTCTTTCGACGAGAGGGAGATGGATCGTGGACTCGACCACGGGGCGTCGAGTGAAGCTTATGTGCGTGAATTGGCCTTCCCACACGCAGAGCATGCTAATCGAAGGCCTCGACCGCAGGGCGTTGAAAGACCTTGCCGACGAGGCAATGAGGTTGAGGTTCAATTGTGTAAGGCTCACATATGCGACTCACATGTTTACTCGCTATGCTAATAGGACGGTTGAAGAGAATTTTGACCTTCTTGATTTGAAAGCTTCTAAGGCTGGATTGGCCTTTGAAGCTGCGGTTGATGTGCTTGGGATGGCTGGTTTAATGGTGATAGCTGACAATCATATTACCCAACCAACATGGTGTTGCTCTCTTCACGATGGAAATGGTTTCTTTGGAGATCGCTATTTCGACCCCCAAGAATGGTTGGAAGCTCTTAGTTTGGTTGCTTCGCGGTTTTGCAACAAATCAACTGTGGTAGCCATGAGTCTACGAAATGAGCTCCGAGGAACAAAATCGAACTCAAAAGATTGGAATACATACATAACACAAGGAGCAACAACTATCCACAACATAAATCCAAACATCCTCGTAATCGTTTCGGGTCTAAACTTCGACAACGACCTAAGATGCCAAAGGCAAAATCCCTTGGCACTAAACAACAACCTACACAAGAAGCTAGTTTTTGAAGTGCACTTGTATTCTTTCAGTGGAGACTCCCAAACAAAGTTCATAAAAAATCCACTCAACAAAATATGCTCAAAGATCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGCGAGTGAGTTTGGGTTTGACCAAAGGGGAGTGAATGAGGCTGATAATAGGTTTTTGAGTTGCTTTCTTGCTCATCTTGCTAAGATGGATTTTGATTGGGCGCTATGGTCTTGGCAAGGGAGTTATTATTATCGAGAGGGTCACGCTCGATCGGATGAAGTGTTTGGAGTCTTGAATTACAATTGGAGTGATGTTAGAAACCCTCGTTTTTCTCAGATGTTTCAGCTATTGCAAACCATGTTGCAAGATCCTAATTCCAATTCCCCAAACTCTTATGTAATGTATCATCCACAAAGTGGACAATGTGTCCAAGTGAAAGACATGATGAGTAAGGAAATTTATCTGAACAACTGTTCTAAGGCAAGCCATTGGAGTCATGATGGAGATGGGACACCAATCATGTTGGAGGGCACTGGTTTGTGCCTAAAAGCCATTGGAAATGGGCTTCAACCCTTACTTTCAAGGGACTGTTTGGGTGAGCAAAGTGCTTGGAAAGCCATTTCAAACTCTAAGCTTCATTTGGCCACACTCACAAAACAAGGGAATATTGGTTTGTGTTTAGAGAAATTAGAGAGCTCAAATTCAACTAGGGTTGTGATGGGCAACTGTGTTTGTGTTGGTGATGATTCAAATTGCTTACAAGATACTCAACCCCAATGGTTTGAACTTGTTCTCACAAATACTTTGTAA

Protein sequence

MGSTTESFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIEGLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGLAFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEALSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNFDNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAEFVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREGHARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKDMMSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAISNSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVLTNTL
Homology
BLAST of Tan0014744 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.8e-109
Identity = 227/544 (41.73%), Postives = 308/544 (56.62%), Query Frame = 0

Query: 12  ALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIEGLDRRALKDLA 71
           AL+ L  +   +SLPL TRGRWIVD  TG RVKL CVNW  H +  L EGL+R  +  +A
Sbjct: 16  ALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVATVA 75

Query: 72  DEAMRLRFNCVRLTYATHMFTR--YANRTVEENFDLLDLKASKAGL-------------- 131
                L FNCVRLTY+ HM TR  Y N TV + F  L+L  + +G+              
Sbjct: 76  HTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGHVA 135

Query: 132 AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEALSLVASRFC 191
           A+   V  L  AG+MVI DNH+++P WCC++ DGNGFFGDRYF+P  W+E L L+A+ F 
Sbjct: 136 AYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATYFN 195

Query: 192 NKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNFDNDLRCQRQ 251
           N   VVAMSLRNELRG +S    W+ ++  GA T+H  NP +LVI+SGL FD DL     
Sbjct: 196 NTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPV 255

Query: 252 NPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAEFVMEGAE-- 311
            P+ L     +K+V+E H YSF    +T       N +C      F     FV   A   
Sbjct: 256 LPVTL--PFKEKIVYEGHWYSFGVPWRTGL----PNDVCKNETGRFKSNVGFVTSSANAT 315

Query: 312 AVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREGH---ARSD 371
           A PLF SEFG DQR VN+ DNR+L+C LA+LA+ D DWALW+  GSYYYR         +
Sbjct: 316 AAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFE 375

Query: 372 EVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPN-SNSPNSYVMYHPQSGQCVQVKDMMSK 431
           E +G  N++WS +RNP F    + +Q  +QDP  +  P   ++YHP SG CV+    +  
Sbjct: 376 ETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVE--SGIGN 435

Query: 432 EIYLNNC-SKASHWSHDGD-GTPIMLEGTGLCLKAIGNGLQPLLSRDCLG-EQSAWKAIS 491
            ++L +C S  S W++D     PI L G+  C+   GNGL  +++ +C     + W  +S
Sbjct: 436 TVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVS 495

Query: 492 NSKLHLAT--LTKQG-NIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQP--QWFEL 526
           +++L L T  L K G    +C   L+ S S  +    C+C+  DS+C     P  QWF++
Sbjct: 496 SAQLQLGTRVLGKDGKEKWMC---LDGSKSPLISTNECICI-TDSHCYPKLNPEKQWFKV 547

BLAST of Tan0014744 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 4.7e-09
Identity = 90/384 (23.44%), Postives = 150/384 (39.06%), Query Frame = 0

Query: 32  RWIVDSTTGRRVKLMCVN-WPSHTQSMLIEGLDRRALKDLADEAMRLRFNCVRLTYATHM 91
           R IVD  +G+ V+L  VN +   T + ++ GL  R  KD+  +   L FN VRL +    
Sbjct: 32  RQIVDD-SGKVVQLKGVNVFGFETGNHVMHGLWARNWKDMIVQMQGLGFNAVRLPFCPAT 91

Query: 92  F---TRYANRTVEENFDLLDLKASKAGLAFEAAVDVLGMAGLMVIADNHITQPTWCCSLH 151
               T  A+     N DL  L + +     +  +      G+ V+ D+H      C  + 
Sbjct: 92  LRSDTMPASIDYSRNADLQGLTSLQ---ILDKVIAEFNARGMYVLLDHHTPD---CAGIS 151

Query: 152 DGNGFFGDRYFDPQEWLEALSLVASRFCNKSTVVAMSLRNELR-----GTKSNSKDWNTY 211
           +   ++   Y + Q WL  L  VA+R+ N   V+ + L+NE       GT + + DWN  
Sbjct: 152 E--LWYTGSYTEAQ-WLADLRFVANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKA 211

Query: 212 ITQGATTIHNINPNILVIVSGLNFDNDLRCQRQNPLALNNNLH-----------KKLVFE 271
             +G+  +  + P  L+ V G+  DN + C     +    NL             +L+  
Sbjct: 212 AERGSAAVLAVAPKWLIAVEGIT-DNPV-CSTNGGIFWGGNLQPLACTPLNIPANRLLLA 271

Query: 272 VHLY-------SFSGDSQTKFIKNPLNKICSKIINGFVERAEFVMEGAEAVPLFASEFGF 331
            H+Y       S+  DS      N +  I  +    F         G  A  L   EFG 
Sbjct: 272 PHVYGPDVFVQSYFNDSN---FPNNMPAIWERHFGQFA--------GTHA--LLLGEFGG 331

Query: 332 DQRGVNEADNRFLSCFLAHLAKMDFDWAL-WSWQGSYYYREGHARSDEVFGVLNYNWSDV 388
                +  D  +    + +L     +    WSW         +  S +  G+L  +W+ V
Sbjct: 332 KYGEGDARDKTWQDALVKYLRSKGINQGFYWSW---------NPNSGDTGGILRDDWTSV 377

BLAST of Tan0014744 vs. NCBI nr
Match: XP_038875504.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 902.5 bits (2331), Expect = 1.7e-258
Identity = 445/541 (82.26%), Postives = 474/541 (87.62%), Query Frame = 0

Query: 1   MGSTTE-SFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLI 60
           MG+TT+ +FVFLA I  F SLSY S PLST GRWIVDS  GRR+KLMCVNWPSHTQSMLI
Sbjct: 1   MGNTTQINFVFLAFISFF-SLSY-SFPLSTNGRWIVDS-AGRRMKLMCVNWPSHTQSMLI 60

Query: 61  EGLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGL---- 120
           EGLDRR LKDLADE +RLRFNCVRLTYATHMFTRYANRTV+ENFDLLDLKASK GL    
Sbjct: 61  EGLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHN 120

Query: 121 ----------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLE 180
                     A+EAAVD+LG +GLMVIADNHI+QP WCCSL DGNGFFGDRYFDP+EWLE
Sbjct: 121 PFILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLE 180

Query: 181 ALSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLN 240
            L LVA RF NKS VVAMSLRNELRGTKS SKDWN Y+TQGATTIH INPNIL+I+SGLN
Sbjct: 181 GLRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLN 240

Query: 241 FDNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERA 300
           FDNDLRCQRQNPL L NNLH KLVFEVHLYSFSG+SQ+KFI NPLNKICSKIINGFVERA
Sbjct: 241 FDNDLRCQRQNPLPL-NNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERA 300

Query: 301 EFVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREG 360
           EFVMEGAEAVPLF SEFGFDQRGVNEAD+RFLSCF AHLAK D DWALW+WQGSYYYR+G
Sbjct: 301 EFVMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQG 360

Query: 361 HARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKD 420
               +EVFGVLNYNW+DVRNP FSQMFQLLQTMLQDPNSNS N+YVMYHPQSGQCVQVKD
Sbjct: 361 KIEPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKD 420

Query: 421 MMSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAI 480
           MM K+IYLNNCS ASHWSH+GDGTPIMLE T  CLKA GNGL P LS DC GEQS W AI
Sbjct: 421 MMDKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAI 480

Query: 481 SNSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVLTN 527
           S+SKLHLATLTKQGN GLCLEK ESSNSTR+VMG+CVCVG+DSNCLQDTQ QWFELV+TN
Sbjct: 481 SDSKLHLATLTKQGNNGLCLEK-ESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTN 536

BLAST of Tan0014744 vs. NCBI nr
Match: XP_023534098.1 (uncharacterized protein LOC111795760 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 898.3 bits (2320), Expect = 3.2e-257
Identity = 440/541 (81.33%), Postives = 477/541 (88.17%), Query Frame = 0

Query: 1   MGSTTESFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIE 60
           MGS T++ + LA I LFSSLS NSLPLSTRGRWIVDSTTGRRVKL+CVNWPSHTQSMLIE
Sbjct: 1   MGSPTQTLMLLAFISLFSSLS-NSLPLSTRGRWIVDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGL----- 120
           GL  R+LKDLADE + L+FNCVRLTYATHMFTRYANRTVEENFDLLDL+ASKAGL     
Sbjct: 61  GLAHRSLKDLADELVSLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKAGLVLHNP 120

Query: 121 ---------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEA 180
                    A+EA VDVLG +GLMVIADNHI+QP WCCSL+DGNGFFGDRYFDP+EWLE 
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPEEWLEG 180

Query: 181 LSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNF 240
           L LVA RF NKS VVAMSLRNELRG KS+SKDWN Y+TQGATTIH+INPN+LVIVSGLNF
Sbjct: 181 LRLVARRFTNKSNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 240

Query: 241 DNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAE 300
           DNDLRCQR NPL L NNLH KLVFEVHLYSFSG ++TKFI NPLNKICS IINGFVERAE
Sbjct: 241 DNDLRCQRHNPLLL-NNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAE 300

Query: 301 FVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREGH 360
           FVM+GAEAVPLF SEFGFDQRG N AD+RFLSCF+AHLAK D DWALW+WQGSYYYR+G 
Sbjct: 301 FVMQGAEAVPLFVSEFGFDQRGTNVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQ 360

Query: 361 ARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKDM 420
           A+SDEVFGVLNYNWSDVRNPRFS+ FQLLQTML+DPNSN+PNSYVMYHPQSGQCV+VKDM
Sbjct: 361 AQSDEVFGVLNYNWSDVRNPRFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDM 420

Query: 421 MSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAIS 480
           MSKEIYLN+CS ASHWSH GDGTPI LE TGLCLKA G+GL+PLLSRDC   +S+W  IS
Sbjct: 421 MSKEIYLNDCSNASHWSHRGDGTPIELEATGLCLKADGDGLRPLLSRDCSSNESSWTTIS 480

Query: 481 NSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVLTNT 528
           NSKLHLATLT+QGN GLCLEK ESSNSTR+VMG CVCVGDDSNCL DT+ QWFELV TNT
Sbjct: 481 NSKLHLATLTRQGN-GLCLEK-ESSNSTRIVMGRCVCVGDDSNCLDDTRSQWFELVATNT 537

BLAST of Tan0014744 vs. NCBI nr
Match: KAG6605660.1 (hypothetical protein SDJN03_02977, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 890.6 bits (2300), Expect = 6.7e-255
Identity = 436/541 (80.59%), Postives = 473/541 (87.43%), Query Frame = 0

Query: 1   MGSTTESFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIE 60
           MGS T++F+ LA I LFSSLS NSLPLSTRGRWIVDS TGRRVKL+CVNWPSHTQSMLIE
Sbjct: 13  MGSPTQTFMLLAFISLFSSLS-NSLPLSTRGRWIVDSITGRRVKLVCVNWPSHTQSMLIE 72

Query: 61  GLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGL----- 120
           GL  R LKDLADE + L+FNCVRLTYATHMFTRYANRTVEENFDLLDL+ASKAGL     
Sbjct: 73  GLANRPLKDLADELVSLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKAGLASHNP 132

Query: 121 ---------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEA 180
                    A+E  VDVLG +GLMVIADNHI+QP WCCSL+DGNGFFGDRYFDPQEWLE 
Sbjct: 133 FVLNMTIFEAYETVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEG 192

Query: 181 LSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNF 240
           L LVA RF NKS VVAMSLRNELRG KS+SKDWN Y+TQGATTIH+INPN+LVIVSGLNF
Sbjct: 193 LRLVARRFTNKSNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 252

Query: 241 DNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAE 300
           DNDLRCQR NPL L NNLH KLVFEVHLYSFSG ++TKFI NPLNKICS IINGFVERAE
Sbjct: 253 DNDLRCQRHNPLPL-NNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAE 312

Query: 301 FVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREGH 360
           FVM+GAEAVPLF SEFGFDQRG N AD+RFLSCF+AHLAK D DWALW+WQGSYYYR+G 
Sbjct: 313 FVMQGAEAVPLFVSEFGFDQRGTNVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQ 372

Query: 361 ARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKDM 420
           A+SDEVFG+LNYNWS VRNPRFS  FQLLQTML+DPNSN+PNSYVMYHPQSGQCV+VK+M
Sbjct: 373 AQSDEVFGILNYNWSGVRNPRFSTTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKEM 432

Query: 421 MSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAIS 480
           MSKEIYLN+CS ASHWSH GDGTPI LE TGLCLKA G+GL+PLLSRDC   +S+W  IS
Sbjct: 433 MSKEIYLNDCSNASHWSHRGDGTPIELEATGLCLKADGDGLRPLLSRDCSSNESSWTTIS 492

Query: 481 NSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVLTNT 528
           NSKLHLATLT+QGN GLCLEK E+SNSTR+VMG CVCVGDDSNCL DT+ QWFELV TNT
Sbjct: 493 NSKLHLATLTRQGN-GLCLEK-ENSNSTRIVMGRCVCVGDDSNCLDDTRSQWFELVATNT 549

BLAST of Tan0014744 vs. NCBI nr
Match: XP_022958497.1 (uncharacterized protein LOC111459707 [Cucurbita moschata])

HSP 1 Score: 888.6 bits (2295), Expect = 2.5e-254
Identity = 434/541 (80.22%), Postives = 473/541 (87.43%), Query Frame = 0

Query: 1   MGSTTESFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIE 60
           MGS +++ + LA I LFSSLS NSLPLSTRGRWIVDSTTGRRVKL+CVNWPSHTQSMLIE
Sbjct: 1   MGSPSQTLMLLAFISLFSSLS-NSLPLSTRGRWIVDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGL----- 120
           GL  R LKDLADE + L+FNCVRLTYATHMFTRYANRTVEENFDLLDL+ASK GL     
Sbjct: 61  GLANRPLKDLADELVSLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLASHNP 120

Query: 121 ---------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEA 180
                    A+E  VDVLG +GLMVIADNHI+QP WCCSL+DGNGFFGDRYFDPQEWLE 
Sbjct: 121 FVLNMTIFEAYETVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEG 180

Query: 181 LSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNF 240
           L LVA RF NKS VVAMSLRNELRG KS+SKDWN Y+TQGATTIH+INPN+LVIVSGLNF
Sbjct: 181 LRLVARRFTNKSNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 240

Query: 241 DNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAE 300
           DNDLRCQR NPL L NNLH KLVFEVHLYSFSG ++TKFI NPLNKICS IINGFVERAE
Sbjct: 241 DNDLRCQRHNPLPL-NNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAE 300

Query: 301 FVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREGH 360
           FVM+GAEAVPLF SEFGFDQRG N AD+RFLSCF+AHLAK D DWALW+WQGSYYYR+G 
Sbjct: 301 FVMQGAEAVPLFVSEFGFDQRGTNVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQ 360

Query: 361 ARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKDM 420
           A+ DEVFG+LNYNWS VRNPRFS+ FQLLQTML+DPNSN+PNSYVMYHPQSGQCV+VKDM
Sbjct: 361 AQFDEVFGILNYNWSGVRNPRFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDM 420

Query: 421 MSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAIS 480
           MSKEIYLN+CS ASHWSH GDGTPI LE TGLCLKA G+GL+PLLSRDCL  +S+W  IS
Sbjct: 421 MSKEIYLNDCSNASHWSHRGDGTPIELEATGLCLKADGDGLRPLLSRDCLSNESSWTTIS 480

Query: 481 NSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVLTNT 528
           NSKLHLATLT+ GN GLCLEK +SSNSTR+VMG CVCVGDDSNCL DT+ QWFELV+TNT
Sbjct: 481 NSKLHLATLTRHGN-GLCLEK-DSSNSTRIVMGRCVCVGDDSNCLDDTRSQWFELVVTNT 537

BLAST of Tan0014744 vs. NCBI nr
Match: XP_008467306.1 (PREDICTED: major extracellular endoglucanase-like [Cucumis melo])

HSP 1 Score: 885.6 bits (2287), Expect = 2.2e-253
Identity = 434/542 (80.07%), Postives = 465/542 (85.79%), Query Frame = 0

Query: 1   MGSTTE-SFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLI 60
           MG TT+ SFV LA IC FSSLSY SLPLST GRWIVDS TG RVKL+CVNWPSHTQSMLI
Sbjct: 1   MGITTQFSFVVLAFICFFSSLSY-SLPLSTNGRWIVDSATGHRVKLVCVNWPSHTQSMLI 60

Query: 61  EGLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGL---- 120
           EGLDRR LKDLA+E MRL+FNCVRLTYATHMFTRYANRTVEENFDLLDL+ASK GL    
Sbjct: 61  EGLDRRPLKDLANEVMRLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHN 120

Query: 121 ----------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLE 180
                     A+EA VDVLG +GLMVIADNHI+QP WCCSL DGNGFFGDRYFD +EWLE
Sbjct: 121 PFVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLE 180

Query: 181 ALSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLN 240
            L LVA RF NKS VVAMSLRNELRG  S SKDWN Y+TQGATTIHNINPNILVI+SGLN
Sbjct: 181 GLRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLN 240

Query: 241 FDNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERA 300
           FDNDLRCQRQ PL L NNLH KLVFEVHLYSFSG+SQ+KFI NPLNKICSKIINGFV+RA
Sbjct: 241 FDNDLRCQRQYPLQL-NNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRA 300

Query: 301 EFVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREG 360
           EFVMEGAEAVPLF SEFG DQ GVNEAD+RFLSCF AHL + D DWALW WQGSYYYR+G
Sbjct: 301 EFVMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQG 360

Query: 361 HARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKD 420
               +EVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNS N+Y+MYHPQSGQCVQV D
Sbjct: 361 KVELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHD 420

Query: 421 MMSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAI 480
           M  KEI+LNNCS ASHWS++GDGTPIML  T  CLKA GNGL P LSRDC GEQS W AI
Sbjct: 421 MKQKEIFLNNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSVWTAI 480

Query: 481 SNSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVLTN 528
           S+SKLHLATLTKQGN G+CLEK ESSNS+R++M +CVCVG DSNCLQDTQ QWF+LV+TN
Sbjct: 481 SDSKLHLATLTKQGNNGMCLEK-ESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTN 539

BLAST of Tan0014744 vs. ExPASy TrEMBL
Match: A0A6J1H201 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.2e-254
Identity = 434/541 (80.22%), Postives = 473/541 (87.43%), Query Frame = 0

Query: 1   MGSTTESFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIE 60
           MGS +++ + LA I LFSSLS NSLPLSTRGRWIVDSTTGRRVKL+CVNWPSHTQSMLIE
Sbjct: 1   MGSPSQTLMLLAFISLFSSLS-NSLPLSTRGRWIVDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGL----- 120
           GL  R LKDLADE + L+FNCVRLTYATHMFTRYANRTVEENFDLLDL+ASK GL     
Sbjct: 61  GLANRPLKDLADELVSLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLASHNP 120

Query: 121 ---------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEA 180
                    A+E  VDVLG +GLMVIADNHI+QP WCCSL+DGNGFFGDRYFDPQEWLE 
Sbjct: 121 FVLNMTIFEAYETVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEG 180

Query: 181 LSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNF 240
           L LVA RF NKS VVAMSLRNELRG KS+SKDWN Y+TQGATTIH+INPN+LVIVSGLNF
Sbjct: 181 LRLVARRFTNKSNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 240

Query: 241 DNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAE 300
           DNDLRCQR NPL L NNLH KLVFEVHLYSFSG ++TKFI NPLNKICS IINGFVERAE
Sbjct: 241 DNDLRCQRHNPLPL-NNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAE 300

Query: 301 FVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREGH 360
           FVM+GAEAVPLF SEFGFDQRG N AD+RFLSCF+AHLAK D DWALW+WQGSYYYR+G 
Sbjct: 301 FVMQGAEAVPLFVSEFGFDQRGTNVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQ 360

Query: 361 ARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKDM 420
           A+ DEVFG+LNYNWS VRNPRFS+ FQLLQTML+DPNSN+PNSYVMYHPQSGQCV+VKDM
Sbjct: 361 AQFDEVFGILNYNWSGVRNPRFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDM 420

Query: 421 MSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAIS 480
           MSKEIYLN+CS ASHWSH GDGTPI LE TGLCLKA G+GL+PLLSRDCL  +S+W  IS
Sbjct: 421 MSKEIYLNDCSNASHWSHRGDGTPIELEATGLCLKADGDGLRPLLSRDCLSNESSWTTIS 480

Query: 481 NSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVLTNT 528
           NSKLHLATLT+ GN GLCLEK +SSNSTR+VMG CVCVGDDSNCL DT+ QWFELV+TNT
Sbjct: 481 NSKLHLATLTRHGN-GLCLEK-DSSNSTRIVMGRCVCVGDDSNCLDDTRSQWFELVVTNT 537

BLAST of Tan0014744 vs. ExPASy TrEMBL
Match: A0A6J1K1F7 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 1.0e-253
Identity = 434/541 (80.22%), Postives = 472/541 (87.25%), Query Frame = 0

Query: 1   MGSTTESFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIE 60
           MGS T++F+ LA I LFSSLS NSLPLSTRGRWIVDS TGRRVKL+CVNWPSHTQSMLIE
Sbjct: 1   MGSPTQTFMLLAFISLFSSLS-NSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGL----- 120
           GL  R LKDLADE + L+FNCVRLTYATHMFTRYANRTVEENFDLLDL+ASKAGL     
Sbjct: 61  GLAHRPLKDLADELVNLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKAGLASHNP 120

Query: 121 ---------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEA 180
                    A+EA VDVLG +GLMVIADNHI+QP WCCSL+DGNGFFGDRYFDPQEWLE 
Sbjct: 121 FILNMTIFDAYEAVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEG 180

Query: 181 LSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNF 240
           L LVA RF NK  VVAMSLRNELRG KS+SKDWN Y+TQGATTIH+INPN+LVIVSGLNF
Sbjct: 181 LRLVARRFTNKLNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 240

Query: 241 DNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAE 300
           DNDLRCQR NPL L NNLH KLVFEVHLYSFSG ++TKFI NPLNKICS IINGFVERAE
Sbjct: 241 DNDLRCQRHNPLPL-NNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSAIINGFVERAE 300

Query: 301 FVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREGH 360
           FVM+GAEAVPLF SEFGFDQRG N AD+RF SCF+AHLA+ D DWALW+WQGSYYYR+G 
Sbjct: 301 FVMQGAEAVPLFVSEFGFDQRGTNVADDRFSSCFVAHLARTDLDWALWAWQGSYYYRQGQ 360

Query: 361 ARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKDM 420
           A+SDEVFGVLNYNWSDVRNP FS+ FQLLQTML+DPNSN+PNSYVMYHPQSGQCV+VKDM
Sbjct: 361 AQSDEVFGVLNYNWSDVRNPHFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDM 420

Query: 421 MSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAIS 480
           MSK+IYLN+CS ASHWSH GDGTPI LE T LCLKA  +GL+PLLSRDC G++SAW  IS
Sbjct: 421 MSKDIYLNDCSNASHWSHRGDGTPIELEATSLCLKADRDGLRPLLSRDCSGDESAWTTIS 480

Query: 481 NSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVLTNT 528
           NSKLHLAT T+QGN GLCLEK ESSNSTR+VMG C+CVGDDSNCL DT+ QWFELV TNT
Sbjct: 481 NSKLHLATFTRQGN-GLCLEK-ESSNSTRIVMGRCLCVGDDSNCLDDTRSQWFELVGTNT 537

BLAST of Tan0014744 vs. ExPASy TrEMBL
Match: A0A1S3CTF8 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 1.0e-253
Identity = 434/542 (80.07%), Postives = 465/542 (85.79%), Query Frame = 0

Query: 1   MGSTTE-SFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLI 60
           MG TT+ SFV LA IC FSSLSY SLPLST GRWIVDS TG RVKL+CVNWPSHTQSMLI
Sbjct: 1   MGITTQFSFVVLAFICFFSSLSY-SLPLSTNGRWIVDSATGHRVKLVCVNWPSHTQSMLI 60

Query: 61  EGLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGL---- 120
           EGLDRR LKDLA+E MRL+FNCVRLTYATHMFTRYANRTVEENFDLLDL+ASK GL    
Sbjct: 61  EGLDRRPLKDLANEVMRLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHN 120

Query: 121 ----------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLE 180
                     A+EA VDVLG +GLMVIADNHI+QP WCCSL DGNGFFGDRYFD +EWLE
Sbjct: 121 PFVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLE 180

Query: 181 ALSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLN 240
            L LVA RF NKS VVAMSLRNELRG  S SKDWN Y+TQGATTIHNINPNILVI+SGLN
Sbjct: 181 GLRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLN 240

Query: 241 FDNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERA 300
           FDNDLRCQRQ PL L NNLH KLVFEVHLYSFSG+SQ+KFI NPLNKICSKIINGFV+RA
Sbjct: 241 FDNDLRCQRQYPLQL-NNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRA 300

Query: 301 EFVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREG 360
           EFVMEGAEAVPLF SEFG DQ GVNEAD+RFLSCF AHL + D DWALW WQGSYYYR+G
Sbjct: 301 EFVMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQG 360

Query: 361 HARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKD 420
               +EVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNS N+Y+MYHPQSGQCVQV D
Sbjct: 361 KVELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHD 420

Query: 421 MMSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAI 480
           M  KEI+LNNCS ASHWS++GDGTPIML  T  CLKA GNGL P LSRDC GEQS W AI
Sbjct: 421 MKQKEIFLNNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSVWTAI 480

Query: 481 SNSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVLTN 528
           S+SKLHLATLTKQGN G+CLEK ESSNS+R++M +CVCVG DSNCLQDTQ QWF+LV+TN
Sbjct: 481 SDSKLHLATLTKQGNNGMCLEK-ESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTN 539

BLAST of Tan0014744 vs. ExPASy TrEMBL
Match: A0A5A7UH07 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00380 PE=3 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 8.8e-253
Identity = 434/542 (80.07%), Postives = 465/542 (85.79%), Query Frame = 0

Query: 1   MGSTTE-SFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLI 60
           MG TT+ SFV LA I  FSSLSY SLPLST GRWIVDS TGRRVKL+CVNWPSHTQSMLI
Sbjct: 1   MGITTQFSFVVLAFISFFSSLSY-SLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLI 60

Query: 61  EGLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGL---- 120
           EGLDRR LKDLA+E MRLRFNCVRLTYATHMFTRYANRTVEENFDLLDL+ASK GL    
Sbjct: 61  EGLDRRPLKDLANEVMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHN 120

Query: 121 ----------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLE 180
                     A+EA VDVLG +GLMVIADNHI+QP WCCSL DGNGFFGDRYFD +EWLE
Sbjct: 121 PFVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLE 180

Query: 181 ALSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLN 240
            L LVA RF NKS VVAMSLRNELRG  S SKDWN Y+TQGATTIHNINPNILVI+SGLN
Sbjct: 181 GLRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLN 240

Query: 241 FDNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERA 300
           FDNDLRCQRQ PL L NNLH KLVFEVHLYSFSG+SQ+KFI NPLNKICSKIINGFV+RA
Sbjct: 241 FDNDLRCQRQYPLQL-NNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRA 300

Query: 301 EFVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREG 360
           EFVMEGAEAVPLF SEFG DQ GVNEAD+RFLSCF AHL + D DWALW WQGSYYYR+G
Sbjct: 301 EFVMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQG 360

Query: 361 HARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKD 420
               +EVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNS N+Y+MYHPQSGQCVQV D
Sbjct: 361 KVELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHD 420

Query: 421 MMSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAI 480
           M  KEI+L+NCS ASHWS++GDGTPIML  T  CLKA GNGL P LSRDC GEQS W AI
Sbjct: 421 MKKKEIFLDNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSIWTAI 480

Query: 481 SNSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVLTN 528
           S+SKLHLATLTKQGN G+CLEK ESSNS+R++M +CVCVG DSNCLQDTQ QWF+LV+TN
Sbjct: 481 SDSKLHLATLTKQGNNGMCLEK-ESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTN 539

BLAST of Tan0014744 vs. ExPASy TrEMBL
Match: A0A6J1DXU9 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111025142 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.5e-236
Identity = 412/544 (75.74%), Postives = 455/544 (83.64%), Query Frame = 0

Query: 1   MGSTTESFVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIE 60
           MGST E+FVFLALI L SSLSY SLPLSTRGRWIVDS TGRRVKL+CVNWPSHTQSMLIE
Sbjct: 1   MGSTKETFVFLALISLLSSLSY-SLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRALKDLADEAMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKASKAGL----- 120
           GLDRR LKDLADEA+RLRFNCVRLTYATHMFTRYANRT+EENFDLLDLKASKAGL     
Sbjct: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNP 120

Query: 121 ---------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEA 180
                    A+EAAVDVLG +GLMVIADNHI+QP WCCSL DGNGFFGDRYFDP+EWLE 
Sbjct: 121 FVLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEG 180

Query: 181 LSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNF 240
           L LVA  F NKS+VV MSLRNELRG +SN KDWN +IT+GA  IH INPN LVI+SGLN+
Sbjct: 181 LRLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNY 240

Query: 241 DNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAE 300
           DNDLRCQ Q PL L  NLH KLV EVHLYSFSGDSQ KF +NPLNKICSK++NGFVERA 
Sbjct: 241 DNDLRCQTQAPLPL-GNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAG 300

Query: 301 FVMEGAEAVPLFASEFGFDQR-GVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREG 360
           FV+EG  AVPL  SEFG DQR   NEAD+RF+SCF A+LA+MD DWA+W WQGSYYYR+G
Sbjct: 301 FVVEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQG 360

Query: 361 HARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKD 420
            AR  EVFGVLNY+WS VRNPRFSQ FQLLQTMLQDPNS SPNSY+MYHPQSGQCVQ + 
Sbjct: 361 QARPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQDPNSYSPNSYLMYHPQSGQCVQAEG 420

Query: 421 MMSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQSAWKAI 480
             SKEIY+NNCS AS WSH+GDGTPI L+ +GLCL+A G GL+P LS +CL + S W+AI
Sbjct: 421 -NSKEIYMNNCSHASQWSHEGDGTPIRLKDSGLCLRASGYGLKPSLSTECLYKHSGWRAI 480

Query: 481 SNSKLHLATLTK--QGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELVL 528
           S+SKLHLATL     GN  LCLE+ ESSNSTR+VMG C+CVGDDS+CL DTQ QWF+LV+
Sbjct: 481 SDSKLHLATLAPNGNGNGNLCLER-ESSNSTRIVMGRCICVGDDSSCLHDTQAQWFQLVV 540

BLAST of Tan0014744 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 384.4 bits (986), Expect = 1.5e-106
Identity = 223/545 (40.92%), Postives = 312/545 (57.25%), Query Frame = 0

Query: 6   ESFVFLALICLFSSLSYNSLPLSTRGRWIV-DSTTGRRVKLMCVNWPSHTQSMLIEGLDR 65
           E F F+++  L   ++  + P ST  RWIV D   GRRVKL CVNWPSH ++ + EGL +
Sbjct: 2   EKFFFISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSK 61

Query: 66  RALKDLADEAMRLRFNCVRLTYATHMFTR---YANRTVEE-----------------NFD 125
           + L  +A++ + + FNCVRLT+  ++ T     A  TV +                 N  
Sbjct: 62  QPLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPT 121

Query: 126 LLDLKASKAGLAFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWL 185
           +LDL   K   AF+  V  L    +MVI DNHI+QP WCCS +DGNGFFGD++ +PQ W+
Sbjct: 122 ILDLPLIK---AFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWI 181

Query: 186 EALSLVASRFCN-KSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSG 245
           + L  +AS F N  S VV MSLRNELRG K N KDW  Y+ +GA  +H++NPN+LVIVSG
Sbjct: 182 KGLKKMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSG 241

Query: 246 LNFDNDLRCQRQNPLALNNNLHKKLVFEVHLYSF----SGDSQTKFIKNPLNKICSKIIN 305
           LN+  DL   R+ P  +  +  +K+VFE+H Y F     GD+        LNKIC K   
Sbjct: 242 LNYATDLSFLRERPFEV--SFRRKVVFEIHWYGFWNTWEGDN--------LNKICGKETE 301

Query: 306 GFVERAEFVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGS 365
             ++ + F++E  + +PLF SEFG DQRG N  DN+FLSCF+A  A  D DW+LW+  GS
Sbjct: 302 KMMKMSGFLLE--KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGS 361

Query: 366 YYYREGHARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQ 425
           YY RE    SDE +GVL++NWS +RN    QM   +QT         P   +M+HP +G 
Sbjct: 362 YYIREKSIGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK-IMFHPSTGL 421

Query: 426 CVQVKDMMSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSRDCLGEQ 485
           C+  K +   ++ L +C+++  W         + E   LCLKA   G    L R    E 
Sbjct: 422 CIVRKSLF--QLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKL-RLFFSES 481

Query: 486 --SAWKAISNSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQ 523
             S WK  S+SK+ L+++TK G   +CL+    +N+  +V  +C C+  +S+C  D + Q
Sbjct: 482 YCSKWKLFSDSKMQLSSITKNG-FSVCLDVDTENNN--IVTNSCKCLRGNSSC--DPRSQ 522

BLAST of Tan0014744 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 368.2 bits (944), Expect = 1.1e-101
Identity = 209/517 (40.43%), Postives = 292/517 (56.48%), Query Frame = 0

Query: 26  PLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIEGLDRRALKDLADEAMRLRFNCVRLT 85
           PLST  RWI+D   G+RVKL CVNWPSH Q ++ EGL ++++ DLA + M + FNCVR T
Sbjct: 4   PLSTNSRWIIDE-KGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 86  YATHMFTRYA---NRTVEENFDLLDLKASKAGL--------------AFEAAVDVLGMAG 145
           +   + T      N TV ++F  L L    +G               A++  V  LG   
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 146 LMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEALSLVASRFCNKSTVVAMSLRNE 205
           +MVI DNH+T+P WCC  +DGNGFFGD +FDP  W+  L+ +A  F   + VV MSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 206 LRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNFDNDLRCQRQNPLALNNNLHKKL 265
           LRG K N  DW  Y+ QGA  +H  NPN+LVI+SGL++D DL   R     +N    +KL
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSR--HVNLTFTRKL 243

Query: 266 VFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAEFVMEGAEAVPLFASEFGFDQRG 325
           VFE+H YSF+ ++ T   KNP N+ C +I+        F +      P+F SEFG D RG
Sbjct: 244 VFELHRYSFT-NTNTWSSKNP-NEACGEILKSIENGGGFNLRD---FPVFLSEFGIDLRG 303

Query: 326 VNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREGHARSDEVFGVLNYNWSDVRNPRF 385
            N  DNR++ C L   A+ D DW++W+ QGSYY REG     E +G+L+ +W  VR+  F
Sbjct: 304 KNVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSF 363

Query: 386 SQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKDMMSKEIYLNNCSKASHWSHDGDG 445
            Q   L+ + LQ P S S    +++HP +G C+    +   ++ L  C+++  WS+    
Sbjct: 364 LQRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQN 423

Query: 446 TPIMLEGTGLCLKAIG-NGLQPLLSRDCLGEQ-SAWKAISNSKLHLATLTKQGNIGLCLE 505
           T + L+   LCL++ G N    L    C     S W+ IS S + LA   K  N  LCL+
Sbjct: 424 T-LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLA--AKSTNNSLCLD 483

Query: 506 KLESSNSTRVVMGNCVCV-GDDSNCLQDTQPQWFELV 523
             E++N   ++  NC CV G+DS+C  D   QWF++V
Sbjct: 484 VDETNN---LMASNCKCVKGEDSSC--DPISQWFKIV 504

BLAST of Tan0014744 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 365.9 bits (938), Expect = 5.3e-101
Identity = 209/538 (38.85%), Postives = 298/538 (55.39%), Query Frame = 0

Query: 8   FVFLALICLFSSLSYN-----SLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIEGL 67
           F+F      FS ++ N     S PLST  RWIVD   G RVKL+C NWPSH Q ++ EGL
Sbjct: 11  FIFPFFCFFFSFIAQNTVPNMSYPLSTSSRWIVDE-NGLRVKLVCANWPSHLQPVVAEGL 70

Query: 68  DRRALKDLADEAMRLRFNCVRLTYATHMFTRYA---NRTVEENFDLLDLKASKAGL---- 127
            ++ +  +A + + + FNCVRLT+   + T      N TV ++F  L L     G     
Sbjct: 71  SKQPVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNN 130

Query: 128 ----------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLE 187
                     A++  V  LG   +MVI DNH+T+P WCC+  DGNGFFGD++FDP  W+ 
Sbjct: 131 PSIIDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVA 190

Query: 188 ALSLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLN 247
           AL  +A+ F   S VV MSLRNELRG K N  DW  Y+ QGA  +H+ N  +LVI+SGL+
Sbjct: 191 ALKKMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLS 250

Query: 248 FDNDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERA 307
           FD DL   R  P+ L  +   KLVFE+H YSFS D  +    NP N IC +++N      
Sbjct: 251 FDADLSFVRSRPVKL--SFTGKLVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGG 310

Query: 308 EFVMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREG 367
            +++   +  PLF SEFG D+RGVN  DNR+  C     A+ D DW+LW+  GSYY R+G
Sbjct: 311 GYLLN--QGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQG 370

Query: 368 HARSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKD 427
               +E +GVL+ +W  VRN  F Q    LQ+ LQ P   +    +++HP +G C+    
Sbjct: 371 KVGMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSL 430

Query: 428 MMSKEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKAIGNGLQPLLSR-DCLGEQSAWKA 487
              K + L  C+ +  WS+      + ++   LCL++ G      ++R  C    S W+ 
Sbjct: 431 DDPKMLTLGPCNSSEPWSYTKKA--LRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQT 490

Query: 488 ISNSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELV 523
           IS S++HLA+ T      LCL+ ++++N+  VV   C C+  D +C  +   QWF+++
Sbjct: 491 ISASRMHLAS-TTSNKTSLCLD-VDTANN--VVANACKCLSKDKSC--EPMSQWFKII 533

BLAST of Tan0014744 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 327.0 bits (837), Expect = 2.8e-89
Identity = 198/535 (37.01%), Postives = 294/535 (54.95%), Query Frame = 0

Query: 8   FVFLALICLFSSLSYNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIEGLDRRAL 67
           F+FL+LI L  +  Y   PL T+ RWIV++  G RVKL C NWPSH + ++ EGL  + +
Sbjct: 12  FLFLSLISLTLATDY---PLFTKSRWIVNN-KGHRVKLACANWPSHLKPVVAEGLSSQPM 71

Query: 68  KDLADEAMRLRFNCVRLTYATHMF---TRYANRTVEENFDLLDLKASKAGL--------- 127
             ++ +   + FNCVRLT+   +    T   N TV+++F+   L     G+         
Sbjct: 72  DSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVN 131

Query: 128 -----AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEALSLV 187
                 F+A V  LG   +MVI DNH T P WCCS  D + FFGD  F+P  W+  L  +
Sbjct: 132 TPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKM 191

Query: 188 ASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNFDNDL 247
           A+ F N   VV MSLRNELRG    SKDW  Y+ +GA  +H  NPN+LVI+SGLNFD DL
Sbjct: 192 ATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADL 251

Query: 248 RCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAEFVME 307
              +  P  +N +  KKLV E+H YSF+ D   ++  + +N  CS++ +       FV++
Sbjct: 252 SFLKDRP--VNLSFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERRTGGFVLD 311

Query: 308 GAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREGHARSD 367
             +  PLF SEFG DQRG +   NR+++C LA  A+ D DWA+W+  G YY+REG     
Sbjct: 312 --QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVV 371

Query: 368 EVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQC-VQVKDMMSK 427
           E +G+L+ NW +V N  + +   ++Q     P     +   ++HP +G C V+       
Sbjct: 372 EAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHCHES 431

Query: 428 EIYLNNCSKASHWSHDGDGTPIMLEGTGLCLK---AIGNGLQPLLSRDCLGEQSAWKAIS 487
           E+ L  C+K   WS+   G   +  G   CL+   A+G  ++  L R C    +  + IS
Sbjct: 432 ELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVK--LGRIC----TKIEQIS 491

Query: 488 NSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFEL 522
            +K+HL+  T  G++ +CL+ ++S N+  VV  +C C+  D+ C  +   QWF++
Sbjct: 492 ATKMHLSFNTSDGSL-VCLD-VDSDNN--VVANSCNCLTGDTTC--EPASQWFKI 525

BLAST of Tan0014744 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 4.7e-73
Identity = 182/539 (33.77%), Postives = 267/539 (49.54%), Query Frame = 0

Query: 7   SFVFLALICLFSSLS--YNSLPLSTRGRWIVDSTTGRRVKLMCVNWPSHTQSMLIEGLDR 66
           SF F       SS S    S PLST+ RWIVD   G+RVKL CVNWP+H Q  + EGL +
Sbjct: 4   SFYFCLFFLFISSTSKLTTSYPLSTKSRWIVDE-KGQRVKLACVNWPAHLQPTVAEGLSK 63

Query: 67  RALKDLADEAMRLRFNCVRLTYATHMFTR---YANRTVEENFDLLDLKASKAGL------ 126
           + L  ++ + + + FNCVRLT+   + T        TV+++F+ L L     G+      
Sbjct: 64  QPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPK 123

Query: 127 --------AFEAAVDVLGMAGLMVIADNHITQPTWCCSLHDGNGFFGDRYFDPQEWLEAL 186
                   AF+  V  LG  G+MVI DNH+T P WCC  +D + FFG  +FDP  W + L
Sbjct: 124 LLHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGL 183

Query: 187 SLVASRFCNKSTVVAMSLRNELRGTKSNSKDWNTYITQGATTIHNINPNILVIVSGLNFD 246
             +A+ F N + V+ MSLRNE RG +     W  ++ QGA  +H  NP +LVI+SG++FD
Sbjct: 184 RKMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFD 243

Query: 247 NDLRCQRQNPLALNNNLHKKLVFEVHLYSFSGDSQTKFIKNPLNKICSKIINGFVERAEF 306
            +L   R    ++N +   KLVFE+H YSFS D +  + K+  N  C KII        F
Sbjct: 244 TNLSFLRDR--SVNVSFTDKLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHNGGF 303

Query: 307 VMEGAEAVPLFASEFGFDQRGVNEADNRFLSCFLAHLAKMDFDWALWSWQGSYYYREGHA 366
           ++      PL  SEFG DQRG + + NR+++C +A  A+ D DWA+W+  G YY R G  
Sbjct: 304 LL--GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTGPG 363

Query: 367 RSDEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSPNSYVMYHPQSGQCVQVKDMM 426
                                                  PN  +++HP +G CV      
Sbjct: 364 L-------------------------------------RPNKNLLFHPSTGLCVTNNPSD 423

Query: 427 S-KEIYLNNCSKASHWSHDGDGTPIMLEGTGLCLKA---IGNGLQPLLSRDCLGEQSAWK 486
           +   + L  C K+  W+ +     + +    +C++A   +G  ++  +   C    S   
Sbjct: 424 NIPTLRLGPCPKSDPWTFNPSEGILWI--NKMCVEAPNVVGQKVKLGVGTKC----SKLG 483

Query: 487 AISNSKLHLATLTKQGNIGLCLEKLESSNSTRVVMGNCVCVGDDSNCLQDTQPQWFELV 523
            IS +K+HL+  T  G + LCL+  E  NS  VV   C  +  D++C  D   QWF+++
Sbjct: 484 QISATKMHLSFKTSNGLL-LCLDVDERDNS--VVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA01.8e-10941.73Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P194874.7e-0923.44Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
Match NameE-valueIdentityDescription
XP_038875504.11.7e-25882.26glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
XP_023534098.13.2e-25781.33uncharacterized protein LOC111795760 [Cucurbita pepo subsp. pepo][more]
KAG6605660.16.7e-25580.59hypothetical protein SDJN03_02977, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022958497.12.5e-25480.22uncharacterized protein LOC111459707 [Cucurbita moschata][more]
XP_008467306.12.2e-25380.07PREDICTED: major extracellular endoglucanase-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1H2011.2e-25480.22Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 P... [more]
A0A6J1K1F71.0e-25380.22Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=... [more]
A0A1S3CTF81.0e-25380.07Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV... [more]
A0A5A7UH078.8e-25380.07Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1DXU91.5e-23675.74Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111025142 ... [more]
Match NameE-valueIdentityDescription
AT3G26130.11.5e-10640.92Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.11.1e-10140.43Cellulase (glycosyl hydrolase family 5) protein [more]
AT1G13130.15.3e-10138.85Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.12.8e-8937.01Glycosyl hydrolase superfamily protein [more]
AT5G16700.14.7e-7333.77Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 66..337
e-value: 7.1E-25
score: 88.0
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 24..381
e-value: 1.0E-63
score: 217.7
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 9..523
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 23..373
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 385..506

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0014744.1Tan0014744.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds