Tan0014577 (gene) Snake gourd v1

BLAST of Tan0014577 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.0e-79
Identity = 155/340 (45.59%), Postives = 205/340 (60.29%), Query Frame = 0

Query: 40  CKLDAIYQLGDSISDTGNLIRENPDSP---FSHLPYGETFFNKSTGR-----------SL 99
           C  D+IYQLGDS SDTGNLIR  PD P    +H PYGETF    TGR           + 
Sbjct: 25  CPFDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFPGTPTGRCSDGRLIIDFIAT 84

Query: 100 AMELPFINPYLDKEAGRSQGVNFAVAGSTALSSEFLAKNKISSSITNSSLTTQLDWMFTY 159
           A+ LP +NPYL +      GVNFAVAG+TAL   FLA   +  S  +S L+ QL+W  TY
Sbjct: 85  ALNLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDIHSHLSAQLNWFRTY 144

Query: 160 FNATCSSQTDCSEKLKSALFLVGEIGGNDYNYALLQGKTIKEVKHLVPEVVQIIKHAVTR 219
             + CS+  +CS KLK+ALF++G IG ND NYA    +TI+E++  VP + + + +A   
Sbjct: 145 LGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNRTIEEIRAYVPFITEAVANATRE 204

Query: 220 IISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSAYNELHCLKNLNDFAKYHNKLLKHAIK 279
           II  GG+R+IVPG FPIGC+   L           ++L CL +LN+ + Y N L + A+ 
Sbjct: 205 IIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDLGCLSSLNNLSIYFNSLFQRALA 264

Query: 280 SLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQTSLQKSCCGIGGDYDFNITQMCGLPG 339
           SL    P   I+YADYY+A+ ++ R    L  + TSL K CCGIGG Y+++  + CG  G
Sbjct: 265 SLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLLKCCCGIGGPYNYDPDRECGSRG 324

Query: 340 VPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFIFPKLYC 366
           VPVCPNP + I WDG HFTQ AY+ +A ++I  I   L C
Sbjct: 325 VPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKALKC 363

BLAST of Tan0014577 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 5.2e-71
Identity = 151/355 (42.54%), Postives = 209/355 (58.87%), Query Frame = 0

Query: 12  TKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHLP 71
           TK L ++V ++ V +  +++        C +++IYQ GDSISDTGNLIR  P S      
Sbjct: 4   TKALSLLVFILFVSLVHSSD-------QCPINSIYQFGDSISDTGNLIRNGPASS----- 63

Query: 72  YGETFFNKSTGRSLAMELPFINPYLDKEAGRSQGVNFAVAGSTALSSEFLAKNKISSSIT 131
                             P   P   +E   +  VNF V+GSTAL+S F ++  +    T
Sbjct: 64  ------------------PTPKPLPQRE--HNVFVNFGVSGSTALNSSFFSERNLHVPAT 123

Query: 132 NSSLTTQLDWMFTYFNATC-SSQTDCSEKLKSALFLVGEIGGNDYNYALLQGKTIKEVKH 191
           N+ L+ QL W   +  +TC  S +DC   LK +LF+VGEIGGNDYNY   QGK ++E++ 
Sbjct: 124 NTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNYGFFQGKPMEEIRS 183

Query: 192 LVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSAYNELHCLKNLN 251
            +P VV  I  A   +I  G   ++VPGNFP+GC PI+LT F  KD+  Y++  CL +LN
Sbjct: 184 YIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDYDDNGCLTHLN 243

Query: 252 DFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQTSLQKSCCGIG 311
           +FA  HN  L+ AI SLRK  P+V IVY DYY+AF +VLR      FD++   KSCCG G
Sbjct: 244 EFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKSVALKSCCGTG 303

Query: 312 GDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFIFPKLYC 366
           G Y+++  +  G  GVPVC NP + ISWDGVH TQKAY++++ +L   I  ++ C
Sbjct: 304 GAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQILSQIKC 320

BLAST of Tan0014577 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 260.4 bits (664), Expect = 3.1e-68
Identity = 148/365 (40.55%), Postives = 216/365 (59.18%), Query Frame = 0

Query: 10  LPTKTLLVVVVVVVVVVEANNNAGGDLVKDCK-LDAIYQLGDSISDTGNL--IRENPDSP 69
           LP   L+  ++  ++V   N+         C+   +I   GDSI+DTGNL  + +  D P
Sbjct: 7   LPAMKLVRFILSTLLVTSVNSQT------QCRNFKSIISFGDSIADTGNLLGLSDPNDLP 66

Query: 70  FSHL-PYGETFFNKSTGR-----------SLAMELPFINPYLD-KEAGRSQGVNFAVAGS 129
            S   PYGETFF+  TGR           +  +  P + P+   + A   +GVNFAVAG+
Sbjct: 67  ASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGA 126

Query: 130 TALSSEFLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGN 189
           TAL   FL +  I S+ITN SL+ QL          C S +DC + +++AL L+GEIGGN
Sbjct: 127 TALEPSFLEERGIHSTITNVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGN 186

Query: 190 DYNYALLQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFK 249
           DYN+AL Q K +KEV+ LVP V+  I  A+T ++  GG   +VPGNFPIG    +LT +K
Sbjct: 187 DYNFALFQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYK 246

Query: 250 TKDSSAYNEL-HCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYA 309
           T +   Y+ L  CLK LNDF++Y+NK L+  +  LRK +P+V I+YADYY+A L + +  
Sbjct: 247 TSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEP 306

Query: 310 SLLEFDQTSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIA 358
           +   F    L  +CCG+GG Y+FN ++ CG  GV  C +P + +++DG+H T+ AY+ I+
Sbjct: 307 AKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLIS 364

BLAST of Tan0014577 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.6e-67
Identity = 143/327 (43.73%), Postives = 193/327 (59.02%), Query Frame = 0

Query: 44  AIYQLGDSISDTGNLI-----RENPDSPFSHLPYGETFFNKSTGRS----LAME------ 103
           +I   GDSI+DTGNL+      + P + F   PYGETFF+  TGRS    + M+      
Sbjct: 31  SIISFGDSIADTGNLVGLSDRNQLPVTAFP--PYGETFFHHPTGRSCDGRIIMDFIAEFV 90

Query: 104 -LPFINPYL-DKEAGRSQGVNFAVAGSTALSSEFLAKNKISSSITNSSLTTQLDWMFTYF 163
            LP++ PY   K     +GVNFAVAG+TAL S FL K  I    TN SL  QL       
Sbjct: 91  GLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFLKKRGIQPH-TNVSLGVQLKSFKKSL 150

Query: 164 NATCSSQTDCSEKLKSALFLVGEIGGNDYNYALLQGKTIKEVKHLVPEVVQIIKHAVTRI 223
              C S +DC + + +AL L+GEIGGNDYN+     K +KEV+ LVP V+  I   +T +
Sbjct: 151 PNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITEL 210

Query: 224 ISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSAYN-ELHCLKNLNDFAKYHNKLLKHAIK 283
           I  GG   +VPG FPIGC  ++LT +KT +   Y+    CLK LN F +YH++ LK  + 
Sbjct: 211 IGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELN 270

Query: 284 SLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQTSLQKSCCGIGGDYDFNITQMCGLPG 343
            LRK +P+V I+YADYY++ L + +  +   F +     +CCGIGG Y+FN T+ CG  G
Sbjct: 271 RLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPF-PACCGIGGPYNFNFTRKCGSVG 330

Query: 344 VPVCPNPDERISWDGVHFTQKAYKYIA 353
           V  C +P + + WDGVH T+ AYK+IA
Sbjct: 331 VKSCKDPSKYVGWDGVHMTEAAYKWIA 353

BLAST of Tan0014577 vs. ExPASy Swiss-Prot
Match: Q3E7I6 (GDSL esterase/lipase At1g28650 OS=Arabidopsis thaliana OX=3702 GN=At1g28650 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 6.1e-64
Identity = 143/374 (38.24%), Postives = 205/374 (54.81%), Query Frame = 0

Query: 1   MAAVLLRKLLPTKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIR 60
           M   LL   L    LL++    +VV  + +          +  +I   GDSI+DTGN + 
Sbjct: 1   MTTTLLMASLIVSFLLILYYTTIVVASSESRCR-------RYKSIISFGDSIADTGNYVH 60

Query: 61  EN-----PDSPFSHLPYGETFFNKSTGR-----------SLAMELPFINPYL-DKEAGRS 120
            +     P + F  LPYGE+FF+  +GR           +  + LP++ PY   +    +
Sbjct: 61  LSNVNNLPQAAF--LPYGESFFHPPSGRYSDGRLVIDFIAEFLGLPYVPPYFGSQNVSFN 120

Query: 121 QGVNFAVAGSTALSSEFLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQT-DCSEKLKS 180
           QG+NFAV G+TAL   FL K  I S  TN SL+ QL+         C+S T DC E L  
Sbjct: 121 QGINFAVYGATALDRAFLVKQGIKSDFTNISLSVQLNTFKQILPNLCASSTRDCREMLGD 180

Query: 181 ALFLVGEIGGNDYNYALLQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPI 240
           +L L+GEIGGNDYNY   +GK+I E+K LVP +++ I  A+  +I  GG   +VPGNFPI
Sbjct: 181 SLILMGEIGGNDYNYPFFEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPI 240

Query: 241 GCLPIFLTQFKTKDSSAYNELHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYY 300
           GC   +LT F+T          C+  LN F ++HN+ LK  +K L+K +P+V I+YADYY
Sbjct: 241 GCSTAYLTLFQTATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYY 300

Query: 301 HAFLWVLRYASLLEFDQTSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVH 357
           ++   + +  +   F    L  +CCG+GG Y+F I + CG  GV  C NP E ++WDG H
Sbjct: 301 NSLYGLFQEPAKYGFKNRPL-AACCGVGGQYNFTIGKECGENGVSYCQNPSEYVNWDGYH 360

BLAST of Tan0014577 vs. NCBI nr
Match: XP_022998964.1 (acetylajmalan esterase-like [Cucurbita maxima])

HSP 1 Score: 480.7 bits (1236), Expect = 1.1e-131
Identity = 229/367 (62.40%), Postives = 293/367 (79.84%), Query Frame = 0

Query: 11  PTKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHL 70
           P+  +L V++V+V V+   ++    L+K C  DAIYQLGDSISDTGNLIR+NP++PFSHL
Sbjct: 8   PSSIVLAVLLVLVSVIGCPSST-AHLLKTCMFDAIYQLGDSISDTGNLIRQNPNTPFSHL 67

Query: 71  PYGETFFNKSTGR-----------SLAMELPFINPYLDKEAGRSQGVNFAVAGSTALSSE 130
           PYGET FNKSTGR           +L   LP +NPYL+K+A    GVNFAVAGSTALSS+
Sbjct: 68  PYGETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGSTALSSQ 127

Query: 131 FLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGNDYNYAL 190
            L++ +I S +TNSSL  QL WMF++FN+ C +Q DC+EKL++ALFLVGEIGGNDYNYAL
Sbjct: 128 LLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGNDYNYAL 187

Query: 191 LQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSA 250
            QGKTI++VK +VP+VVQ IK+AV ++ISYG TR++VPGNFPIGCLPI+LT F+T D++A
Sbjct: 188 FQGKTIQDVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQTNDTTA 247

Query: 251 YNELHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQ 310
           Y+ELHCLK+LN  A YHN  +K AI+ L++ +P+  IVY DYY+A LW+LR A +L FD+
Sbjct: 248 YDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAFILGFDE 307

Query: 311 TSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFI 367
           TSLQKSCCGIGGDY+F++ +MCG+ GV VC NPDER+SWDG+H TQKAYK++AYWLI  I
Sbjct: 308 TSLQKSCCGIGGDYNFSLMKMCGVGGVSVCXNPDERVSWDGIHLTQKAYKFMAYWLIHDI 367

BLAST of Tan0014577 vs. NCBI nr
Match: XP_022946880.1 (acetylajmalan esterase-like [Cucurbita moschata])

HSP 1 Score: 480.3 bits (1235), Expect = 1.5e-131
Identity = 234/364 (64.29%), Postives = 291/364 (79.95%), Query Frame = 0

Query: 14  TLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHLPYG 73
           ++++VV++V+V V   +++    +K C  DAIYQ+GDSISDTGNLI +NP++PFSHLPYG
Sbjct: 10  SIVLVVLLVLVSVHGCSSSKAPSLKACMFDAIYQVGDSISDTGNLILQNPNTPFSHLPYG 69

Query: 74  ETFFNKSTGRS---------LAME--LPFINPYLDKEAGRSQGVNFAVAGSTALSSEFLA 133
           ETFFNKSTGR           AM+  LP +NPYL+K+A    GVNFAVAGSTALSSE L+
Sbjct: 70  ETFFNKSTGRCSNGLLIIDYFAMDAGLPLLNPYLNKDALTRHGVNFAVAGSTALSSEALS 129

Query: 134 KNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGNDYNYALLQG 193
           + KISS +TNSSL  QLDWMF++FN+ C +Q DC EKLK+ALFLVGEIGGNDYNYALLQG
Sbjct: 130 QKKISSLVTNSSLDQQLDWMFSHFNSICYNQRDCIEKLKNALFLVGEIGGNDYNYALLQG 189

Query: 194 KTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSAYNE 253
           KTI EVK +VPEVVQ IK AV R+ISYG T ++VPGNFPIGCLPI+LT+F+T D++AY+E
Sbjct: 190 KTIPEVKDMVPEVVQTIKKAVERVISYGATHVVVPGNFPIGCLPIYLTRFQTNDATAYDE 249

Query: 254 LHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQTSL 313
           LHCLK+LN  A YHN  +K  I+ L+K +P+  IVY DYY+A LW+LR A  L FD+TSL
Sbjct: 250 LHCLKDLNGLAIYHNDQIKQVIEVLKKENPHTVIVYGDYYNALLWILRSAFTLGFDKTSL 309

Query: 314 QKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFIFPK 367
           QKSCCGIGGDY+F++ + CG+ GV VC NPDER+SWDG+H TQKAYK++A WLIQ I P+
Sbjct: 310 QKSCCGIGGDYNFDLMKTCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMADWLIQDILPQ 369

BLAST of Tan0014577 vs. NCBI nr
Match: XP_022946882.1 (GDSL esterase/lipase At5g03980-like [Cucurbita moschata])

HSP 1 Score: 480.3 bits (1235), Expect = 1.5e-131
Identity = 226/367 (61.58%), Postives = 293/367 (79.84%), Query Frame = 0

Query: 11  PTKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHL 70
           P+  +L V++V++ V+   ++    L+K C  D IYQLGDSISDTGNLI +NP++PFSHL
Sbjct: 8   PSPIVLAVLIVLLSVLVCPSST-AHLLKTCMFDTIYQLGDSISDTGNLILQNPNTPFSHL 67

Query: 71  PYGETFFNKSTGR-----------SLAMELPFINPYLDKEAGRSQGVNFAVAGSTALSSE 130
           PYG++FFNKSTGR           +L   LP +NPYL+K+A    GVNFAVAGSTALSS 
Sbjct: 68  PYGQSFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDASTRHGVNFAVAGSTALSSH 127

Query: 131 FLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGNDYNYAL 190
            L++ +I S +TN+SL  QLDWMF++FN+ C +Q DC+EKL++ALFLVGEIGGNDYNYAL
Sbjct: 128 LLSQKQILSPVTNTSLDQQLDWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGNDYNYAL 187

Query: 191 LQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSA 250
            QGKTI+EVK +VP+VVQ IK+AV ++ISYG TR++VPGNFPIGCLPI+LT F+T D++A
Sbjct: 188 FQGKTIQEVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQTNDTTA 247

Query: 251 YNELHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQ 310
           Y+ELHCLK+LND A YHN  +K AI+ L++ +P+  I+Y DYY+A LW+LR A +L FD+
Sbjct: 248 YDELHCLKDLNDLATYHNDQIKQAIQVLKRENPHSVIIYGDYYNALLWILRRAFILGFDE 307

Query: 311 TSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFI 367
            SLQKSCCGIGGDY+F++ +MCG+ GV VC NPDER+SWDG+H TQKAYK++AYWLI  I
Sbjct: 308 ASLQKSCCGIGGDYNFSLVKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAYWLIHDI 367

BLAST of Tan0014577 vs. NCBI nr
Match: XP_022998963.1 (acetylajmalan esterase-like [Cucurbita maxima] >XP_022998972.1 acetylajmalan esterase-like [Cucurbita maxima])

HSP 1 Score: 479.2 bits (1232), Expect = 3.3e-131
Identity = 229/367 (62.40%), Postives = 292/367 (79.56%), Query Frame = 0

Query: 11  PTKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHL 70
           P+  +L V++V+V V+   ++    L+K C  DAIYQLGDSISDTGNLIR+NP++PFSHL
Sbjct: 8   PSSIVLAVLLVLVSVIGCPSST-AHLLKTCMFDAIYQLGDSISDTGNLIRQNPNTPFSHL 67

Query: 71  PYGETFFNKSTGR-----------SLAMELPFINPYLDKEAGRSQGVNFAVAGSTALSSE 130
           PYGET FNKSTGR           +L   LP +NPYL+K+A    GVNFAVAGSTALSS+
Sbjct: 68  PYGETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGSTALSSQ 127

Query: 131 FLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGNDYNYAL 190
            L++ +I S +TNSSL  QL WMF++FN+ C +Q DC+EKL++ALFLVGEIGGNDYNYAL
Sbjct: 128 LLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGNDYNYAL 187

Query: 191 LQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSA 250
            QGKTI++VK +VP+VVQ IK+AV + ISYG TR++VPGNFPIGCLPI+LT F+T D++A
Sbjct: 188 FQGKTIQDVKDMVPQVVQTIKNAVEKAISYGATRVVVPGNFPIGCLPIYLTGFQTNDTTA 247

Query: 251 YNELHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQ 310
           Y+ELHCLK+LN  A YHN  +K AI+ L++ +P+  IVY DYY+A LW+LR A +L FD+
Sbjct: 248 YDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAFILGFDE 307

Query: 311 TSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFI 367
           TSLQKSCCGIGGDY+F++ +MCG+ GV VC NPDER+SWDG+H TQKAYK++AYWLI  I
Sbjct: 308 TSLQKSCCGIGGDYNFSLMKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAYWLIHDI 367

BLAST of Tan0014577 vs. NCBI nr
Match: XP_038877043.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At5g03980-like, partial [Benincasa hispida])

HSP 1 Score: 478.8 bits (1231), Expect = 4.3e-131
Identity = 232/366 (63.39%), Postives = 290/366 (79.23%), Query Frame = 0

Query: 12  TKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHLP 71
           TK+L + + + + +  + N     L+K C  DAIYQLGDSISDTGNLIREN ++PFSHLP
Sbjct: 5   TKSLWISIFLFLFLSSSFN---AHLLKVCMFDAIYQLGDSISDTGNLIRENLNTPFSHLP 64

Query: 72  YGETFFNKSTGR-----------SLAMELPFINPYLDKEAGRSQGVNFAVAGSTALSSEF 131
           YGE+FFN  TGR           +L   LP ++PYL+K+A  + GVNFAVAGSTALSSE 
Sbjct: 65  YGESFFNNPTGRCSNGLLMIDYFALDAGLPLVSPYLNKDALTNHGVNFAVAGSTALSSEL 124

Query: 132 LAKNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGNDYNYALL 191
           L + KISS +TNSSL+ QLDWMF++FN+ C +Q DC+EKL +ALFLVGEIGGNDYNYAL 
Sbjct: 125 LFQKKISSPVTNSSLSHQLDWMFSHFNSICYNQRDCNEKLYNALFLVGEIGGNDYNYALF 184

Query: 192 QGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSAY 251
           QGKTI+EVK +VP+VVQ IK AV R+ISYG TR++VPGNFPIGC PI+LT F+T D+SAY
Sbjct: 185 QGKTIQEVKDMVPDVVQSIKSAVERVISYGATRVVVPGNFPIGCFPIYLTAFQTNDTSAY 244

Query: 252 NELHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQT 311
           +ELHCLK+LN FA YHN  +K AI+ L+K +P   IVY DYY+AFLW+LR+A +L FD+ 
Sbjct: 245 DELHCLKDLNGFASYHNDQVKEAIEVLKKENPXTVIVYGDYYNAFLWILRHAFVLGFDEG 304

Query: 312 SLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFIF 367
           SLQKSCCG+GG+Y+FN+ +MCGLPGV VC NP+E ISWDGVH TQKAYK++A+WLI  IF
Sbjct: 305 SLQKSCCGVGGNYNFNLMKMCGLPGVEVCQNPNEHISWDGVHLTQKAYKFMAHWLIHAIF 364

BLAST of Tan0014577 vs. ExPASy TrEMBL
Match: A0A6J1KFS3 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493492 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 5.5e-132
Identity = 229/367 (62.40%), Postives = 293/367 (79.84%), Query Frame = 0

Query: 11  PTKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHL 70
           P+  +L V++V+V V+   ++    L+K C  DAIYQLGDSISDTGNLIR+NP++PFSHL
Sbjct: 8   PSSIVLAVLLVLVSVIGCPSST-AHLLKTCMFDAIYQLGDSISDTGNLIRQNPNTPFSHL 67

Query: 71  PYGETFFNKSTGR-----------SLAMELPFINPYLDKEAGRSQGVNFAVAGSTALSSE 130
           PYGET FNKSTGR           +L   LP +NPYL+K+A    GVNFAVAGSTALSS+
Sbjct: 68  PYGETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGSTALSSQ 127

Query: 131 FLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGNDYNYAL 190
            L++ +I S +TNSSL  QL WMF++FN+ C +Q DC+EKL++ALFLVGEIGGNDYNYAL
Sbjct: 128 LLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGNDYNYAL 187

Query: 191 LQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSA 250
            QGKTI++VK +VP+VVQ IK+AV ++ISYG TR++VPGNFPIGCLPI+LT F+T D++A
Sbjct: 188 FQGKTIQDVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQTNDTTA 247

Query: 251 YNELHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQ 310
           Y+ELHCLK+LN  A YHN  +K AI+ L++ +P+  IVY DYY+A LW+LR A +L FD+
Sbjct: 248 YDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAFILGFDE 307

Query: 311 TSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFI 367
           TSLQKSCCGIGGDY+F++ +MCG+ GV VC NPDER+SWDG+H TQKAYK++AYWLI  I
Sbjct: 308 TSLQKSCCGIGGDYNFSLMKMCGVGGVSVCXNPDERVSWDGIHLTQKAYKFMAYWLIHDI 367

BLAST of Tan0014577 vs. ExPASy TrEMBL
Match: A0A6J1G595 (acetylajmalan esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111450820 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 7.1e-132
Identity = 234/364 (64.29%), Postives = 291/364 (79.95%), Query Frame = 0

Query: 14  TLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHLPYG 73
           ++++VV++V+V V   +++    +K C  DAIYQ+GDSISDTGNLI +NP++PFSHLPYG
Sbjct: 10  SIVLVVLLVLVSVHGCSSSKAPSLKACMFDAIYQVGDSISDTGNLILQNPNTPFSHLPYG 69

Query: 74  ETFFNKSTGRS---------LAME--LPFINPYLDKEAGRSQGVNFAVAGSTALSSEFLA 133
           ETFFNKSTGR           AM+  LP +NPYL+K+A    GVNFAVAGSTALSSE L+
Sbjct: 70  ETFFNKSTGRCSNGLLIIDYFAMDAGLPLLNPYLNKDALTRHGVNFAVAGSTALSSEALS 129

Query: 134 KNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGNDYNYALLQG 193
           + KISS +TNSSL  QLDWMF++FN+ C +Q DC EKLK+ALFLVGEIGGNDYNYALLQG
Sbjct: 130 QKKISSLVTNSSLDQQLDWMFSHFNSICYNQRDCIEKLKNALFLVGEIGGNDYNYALLQG 189

Query: 194 KTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSAYNE 253
           KTI EVK +VPEVVQ IK AV R+ISYG T ++VPGNFPIGCLPI+LT+F+T D++AY+E
Sbjct: 190 KTIPEVKDMVPEVVQTIKKAVERVISYGATHVVVPGNFPIGCLPIYLTRFQTNDATAYDE 249

Query: 254 LHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQTSL 313
           LHCLK+LN  A YHN  +K  I+ L+K +P+  IVY DYY+A LW+LR A  L FD+TSL
Sbjct: 250 LHCLKDLNGLAIYHNDQIKQVIEVLKKENPHTVIVYGDYYNALLWILRSAFTLGFDKTSL 309

Query: 314 QKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFIFPK 367
           QKSCCGIGGDY+F++ + CG+ GV VC NPDER+SWDG+H TQKAYK++A WLIQ I P+
Sbjct: 310 QKSCCGIGGDYNFDLMKTCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMADWLIQDILPQ 369

BLAST of Tan0014577 vs. ExPASy TrEMBL
Match: A0A6J1G4V8 (GDSL esterase/lipase At5g03980-like OS=Cucurbita moschata OX=3662 GN=LOC111450822 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 7.1e-132
Identity = 226/367 (61.58%), Postives = 293/367 (79.84%), Query Frame = 0

Query: 11  PTKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHL 70
           P+  +L V++V++ V+   ++    L+K C  D IYQLGDSISDTGNLI +NP++PFSHL
Sbjct: 8   PSPIVLAVLIVLLSVLVCPSST-AHLLKTCMFDTIYQLGDSISDTGNLILQNPNTPFSHL 67

Query: 71  PYGETFFNKSTGR-----------SLAMELPFINPYLDKEAGRSQGVNFAVAGSTALSSE 130
           PYG++FFNKSTGR           +L   LP +NPYL+K+A    GVNFAVAGSTALSS 
Sbjct: 68  PYGQSFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDASTRHGVNFAVAGSTALSSH 127

Query: 131 FLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGNDYNYAL 190
            L++ +I S +TN+SL  QLDWMF++FN+ C +Q DC+EKL++ALFLVGEIGGNDYNYAL
Sbjct: 128 LLSQKQILSPVTNTSLDQQLDWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGNDYNYAL 187

Query: 191 LQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSA 250
            QGKTI+EVK +VP+VVQ IK+AV ++ISYG TR++VPGNFPIGCLPI+LT F+T D++A
Sbjct: 188 FQGKTIQEVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQTNDTTA 247

Query: 251 YNELHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQ 310
           Y+ELHCLK+LND A YHN  +K AI+ L++ +P+  I+Y DYY+A LW+LR A +L FD+
Sbjct: 248 YDELHCLKDLNDLATYHNDQIKQAIQVLKRENPHSVIIYGDYYNALLWILRRAFILGFDE 307

Query: 311 TSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFI 367
            SLQKSCCGIGGDY+F++ +MCG+ GV VC NPDER+SWDG+H TQKAYK++AYWLI  I
Sbjct: 308 ASLQKSCCGIGGDYNFSLVKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAYWLIHDI 367

BLAST of Tan0014577 vs. ExPASy TrEMBL
Match: A0A6J1K9I7 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493500 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.6e-131
Identity = 229/367 (62.40%), Postives = 292/367 (79.56%), Query Frame = 0

Query: 11  PTKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHL 70
           P+  +L V++V+V V+   ++    L+K C  DAIYQLGDSISDTGNLIR+NP++PFSHL
Sbjct: 8   PSSIVLAVLLVLVSVIGCPSST-AHLLKTCMFDAIYQLGDSISDTGNLIRQNPNTPFSHL 67

Query: 71  PYGETFFNKSTGR-----------SLAMELPFINPYLDKEAGRSQGVNFAVAGSTALSSE 130
           PYGET FNKSTGR           +L   LP +NPYL+K+A    GVNFAVAGSTALSS+
Sbjct: 68  PYGETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGSTALSSQ 127

Query: 131 FLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGNDYNYAL 190
            L++ +I S +TNSSL  QL WMF++FN+ C +Q DC+EKL++ALFLVGEIGGNDYNYAL
Sbjct: 128 LLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGNDYNYAL 187

Query: 191 LQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSA 250
            QGKTI++VK +VP+VVQ IK+AV + ISYG TR++VPGNFPIGCLPI+LT F+T D++A
Sbjct: 188 FQGKTIQDVKDMVPQVVQTIKNAVEKAISYGATRVVVPGNFPIGCLPIYLTGFQTNDTTA 247

Query: 251 YNELHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQ 310
           Y+ELHCLK+LN  A YHN  +K AI+ L++ +P+  IVY DYY+A LW+LR A +L FD+
Sbjct: 248 YDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAFILGFDE 307

Query: 311 TSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFI 367
           TSLQKSCCGIGGDY+F++ +MCG+ GV VC NPDER+SWDG+H TQKAYK++AYWLI  I
Sbjct: 308 TSLQKSCCGIGGDYNFSLMKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAYWLIHDI 367

BLAST of Tan0014577 vs. ExPASy TrEMBL
Match: A0A6J1KFS8 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493497 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.7e-131
Identity = 234/367 (63.76%), Postives = 290/367 (79.02%), Query Frame = 0

Query: 11  PTKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHL 70
           P   +LVV++V+V V+  +++    L K C  DAIYQ+GDSISDTGNLI +NP++PFSHL
Sbjct: 8   PLSIVLVVLIVLVSVLGCSSSKAPSL-KACMFDAIYQVGDSISDTGNLILQNPNTPFSHL 67

Query: 71  PYGETFFNKSTGR-----------SLAMELPFINPYLDKEAGRSQGVNFAVAGSTALSSE 130
           PYGETFFNKSTGR           +L   LP +NPYL+K+A    GVNFAVAGSTALSSE
Sbjct: 68  PYGETFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGSTALSSE 127

Query: 131 FLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGNDYNYAL 190
            L++ KISS +TNSSL  QLDWMF++FN+ C  Q DC EKLK+ALFLVGEIGGNDYNYAL
Sbjct: 128 ALSQKKISSLVTNSSLDQQLDWMFSHFNSICYDQIDCIEKLKNALFLVGEIGGNDYNYAL 187

Query: 191 LQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSA 250
           LQGKT +EVK +V EVVQ IK+AV R+ISYG TR++VPGNFPIGCLPI+LT+F+T D+ A
Sbjct: 188 LQGKTNQEVKDMVSEVVQTIKNAVERVISYGATRVVVPGNFPIGCLPIYLTRFQTNDTIA 247

Query: 251 YNELHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQ 310
           Y+ELHCLK+LND A YHN  +K  I+ L+K +P+  IVY DYY+A LW+LR A +L FD+
Sbjct: 248 YDELHCLKDLNDLAIYHNDQIKQVIEVLKKENPHTVIVYGDYYNALLWILRRAFILGFDK 307

Query: 311 TSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFI 367
           TSLQKSCCGIGG+Y+F++ + CG+ GV VC NPD+R+SWDGVH TQKAYK++A WLI  I
Sbjct: 308 TSLQKSCCGIGGEYNFDLMKTCGVSGVSVCSNPDDRVSWDGVHLTQKAYKFMADWLIHDI 367

BLAST of Tan0014577 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 269.6 bits (688), Expect = 3.7e-72
Identity = 151/355 (42.54%), Postives = 209/355 (58.87%), Query Frame = 0

Query: 12  TKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIRENPDSPFSHLP 71
           TK L ++V ++ V +  +++        C +++IYQ GDSISDTGNLIR  P S      
Sbjct: 4   TKALSLLVFILFVSLVHSSD-------QCPINSIYQFGDSISDTGNLIRNGPASS----- 63

Query: 72  YGETFFNKSTGRSLAMELPFINPYLDKEAGRSQGVNFAVAGSTALSSEFLAKNKISSSIT 131
                             P   P   +E   +  VNF V+GSTAL+S F ++  +    T
Sbjct: 64  ------------------PTPKPLPQRE--HNVFVNFGVSGSTALNSSFFSERNLHVPAT 123

Query: 132 NSSLTTQLDWMFTYFNATC-SSQTDCSEKLKSALFLVGEIGGNDYNYALLQGKTIKEVKH 191
           N+ L+ QL W   +  +TC  S +DC   LK +LF+VGEIGGNDYNY   QGK ++E++ 
Sbjct: 124 NTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNYGFFQGKPMEEIRS 183

Query: 192 LVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSAYNELHCLKNLN 251
            +P VV  I  A   +I  G   ++VPGNFP+GC PI+LT F  KD+  Y++  CL +LN
Sbjct: 184 YIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDYDDNGCLTHLN 243

Query: 252 DFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQTSLQKSCCGIG 311
           +FA  HN  L+ AI SLRK  P+V IVY DYY+AF +VLR      FD++   KSCCG G
Sbjct: 244 EFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKSVALKSCCGTG 303

Query: 312 GDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIAYWLIQFIFPKLYC 366
           G Y+++  +  G  GVPVC NP + ISWDGVH TQKAY++++ +L   I  ++ C
Sbjct: 304 GAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQILSQIKC 320

BLAST of Tan0014577 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 2.2e-69
Identity = 148/365 (40.55%), Postives = 216/365 (59.18%), Query Frame = 0

Query: 10  LPTKTLLVVVVVVVVVVEANNNAGGDLVKDCK-LDAIYQLGDSISDTGNL--IRENPDSP 69
           LP   L+  ++  ++V   N+         C+   +I   GDSI+DTGNL  + +  D P
Sbjct: 7   LPAMKLVRFILSTLLVTSVNSQT------QCRNFKSIISFGDSIADTGNLLGLSDPNDLP 66

Query: 70  FSHL-PYGETFFNKSTGR-----------SLAMELPFINPYLD-KEAGRSQGVNFAVAGS 129
            S   PYGETFF+  TGR           +  +  P + P+   + A   +GVNFAVAG+
Sbjct: 67  ASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGA 126

Query: 130 TALSSEFLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQTDCSEKLKSALFLVGEIGGN 189
           TAL   FL +  I S+ITN SL+ QL          C S +DC + +++AL L+GEIGGN
Sbjct: 127 TALEPSFLEERGIHSTITNVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGN 186

Query: 190 DYNYALLQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPIGCLPIFLTQFK 249
           DYN+AL Q K +KEV+ LVP V+  I  A+T ++  GG   +VPGNFPIG    +LT +K
Sbjct: 187 DYNFALFQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYK 246

Query: 250 TKDSSAYNEL-HCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYYHAFLWVLRYA 309
           T +   Y+ L  CLK LNDF++Y+NK L+  +  LRK +P+V I+YADYY+A L + +  
Sbjct: 247 TSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEP 306

Query: 310 SLLEFDQTSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVHFTQKAYKYIA 358
           +   F    L  +CCG+GG Y+FN ++ CG  GV  C +P + +++DG+H T+ AY+ I+
Sbjct: 307 AKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLIS 364

BLAST of Tan0014577 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 1.1e-68
Identity = 143/327 (43.73%), Postives = 193/327 (59.02%), Query Frame = 0

Query: 44  AIYQLGDSISDTGNLI-----RENPDSPFSHLPYGETFFNKSTGRS----LAME------ 103
           +I   GDSI+DTGNL+      + P + F   PYGETFF+  TGRS    + M+      
Sbjct: 31  SIISFGDSIADTGNLVGLSDRNQLPVTAFP--PYGETFFHHPTGRSCDGRIIMDFIAEFV 90

Query: 104 -LPFINPYL-DKEAGRSQGVNFAVAGSTALSSEFLAKNKISSSITNSSLTTQLDWMFTYF 163
            LP++ PY   K     +GVNFAVAG+TAL S FL K  I    TN SL  QL       
Sbjct: 91  GLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFLKKRGIQPH-TNVSLGVQLKSFKKSL 150

Query: 164 NATCSSQTDCSEKLKSALFLVGEIGGNDYNYALLQGKTIKEVKHLVPEVVQIIKHAVTRI 223
              C S +DC + + +AL L+GEIGGNDYN+     K +KEV+ LVP V+  I   +T +
Sbjct: 151 PNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITEL 210

Query: 224 ISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSAYN-ELHCLKNLNDFAKYHNKLLKHAIK 283
           I  GG   +VPG FPIGC  ++LT +KT +   Y+    CLK LN F +YH++ LK  + 
Sbjct: 211 IGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELN 270

Query: 284 SLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQTSLQKSCCGIGGDYDFNITQMCGLPG 343
            LRK +P+V I+YADYY++ L + +  +   F +     +CCGIGG Y+FN T+ CG  G
Sbjct: 271 RLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPF-PACCGIGGPYNFNFTRKCGSVG 330

Query: 344 VPVCPNPDERISWDGVHFTQKAYKYIA 353
           V  C +P + + WDGVH T+ AYK+IA
Sbjct: 331 VKSCKDPSKYVGWDGVHMTEAAYKWIA 353

BLAST of Tan0014577 vs. TAIR 10
Match: AT1G28650.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 4.4e-65
Identity = 143/374 (38.24%), Postives = 205/374 (54.81%), Query Frame = 0

Query: 1   MAAVLLRKLLPTKTLLVVVVVVVVVVEANNNAGGDLVKDCKLDAIYQLGDSISDTGNLIR 60
           M   LL   L    LL++    +VV  + +          +  +I   GDSI+DTGN + 
Sbjct: 1   MTTTLLMASLIVSFLLILYYTTIVVASSESRCR-------RYKSIISFGDSIADTGNYVH 60

Query: 61  EN-----PDSPFSHLPYGETFFNKSTGR-----------SLAMELPFINPYL-DKEAGRS 120
            +     P + F  LPYGE+FF+  +GR           +  + LP++ PY   +    +
Sbjct: 61  LSNVNNLPQAAF--LPYGESFFHPPSGRYSDGRLVIDFIAEFLGLPYVPPYFGSQNVSFN 120

Query: 121 QGVNFAVAGSTALSSEFLAKNKISSSITNSSLTTQLDWMFTYFNATCSSQT-DCSEKLKS 180
           QG+NFAV G+TAL   FL K  I S  TN SL+ QL+         C+S T DC E L  
Sbjct: 121 QGINFAVYGATALDRAFLVKQGIKSDFTNISLSVQLNTFKQILPNLCASSTRDCREMLGD 180

Query: 181 ALFLVGEIGGNDYNYALLQGKTIKEVKHLVPEVVQIIKHAVTRIISYGGTRIIVPGNFPI 240
           +L L+GEIGGNDYNY   +GK+I E+K LVP +++ I  A+  +I  GG   +VPGNFPI
Sbjct: 181 SLILMGEIGGNDYNYPFFEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPI 240

Query: 241 GCLPIFLTQFKTKDSSAYNELHCLKNLNDFAKYHNKLLKHAIKSLRKNHPNVKIVYADYY 300
           GC   +LT F+T          C+  LN F ++HN+ LK  +K L+K +P+V I+YADYY
Sbjct: 241 GCSTAYLTLFQTATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYY 300

Query: 301 HAFLWVLRYASLLEFDQTSLQKSCCGIGGDYDFNITQMCGLPGVPVCPNPDERISWDGVH 357
           ++   + +  +   F    L  +CCG+GG Y+F I + CG  GV  C NP E ++WDG H
Sbjct: 301 NSLYGLFQEPAKYGFKNRPL-AACCGVGGQYNFTIGKECGENGVSYCQNPSEYVNWDGYH 360

BLAST of Tan0014577 vs. TAIR 10
Match: AT1G28610.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 244.6 bits (623), Expect = 1.3e-64
Identity = 135/332 (40.66%), Postives = 194/332 (58.43%), Query Frame = 0

Query: 42  LDAIYQLGDSISDTGNLI---RENPDSPFSHLPYGETFFNKSTGRSL-----------AM 101
           L++I   GDSI+DTGNL+     N     + LPYGETFF+  TGRS             +
Sbjct: 29  LESIISFGDSITDTGNLVGLSDRNHLPVTAFLPYGETFFHHPTGRSCNGRIIIDFIAEFL 88

Query: 102 ELPFINPYLDKEAGR-SQGVNFAVAGSTALSSEFLAKNKISSSITNSSLTTQLDWMFTYF 161
            LP + P+   + G   +GVNFAVAG+TAL +  L K  I    +N SL  QL       
Sbjct: 89  GLPHVPPFYGSKNGNFEKGVNFAVAGATALETSILEKRGIYYPHSNISLGIQLKTFKESL 148

Query: 162 NATCSSQTDCSEKLKSALFLVGEIGGNDYNYALLQGKTIKEVKHLVPEVVQIIKHAVTRI 221
              C S TDC + + +A  ++GEIGGND+N+A    KT  EVK LVP V+  I  A+  +
Sbjct: 149 PNLCGSPTDCRDMIGNAFIIMGEIGGNDFNFAFFVNKT-SEVKELVPLVITKISSAIVEL 208

Query: 222 ISYGGTRIIVPGNFPIGCLPIFLTQFKTKDSSAYNEL-HCLKNLNDFAKYHNKLLKHAIK 281
           +  GG   +VPGNFP+GC   +LT ++T +   Y+ L  CL  LNDF++Y+N+ L+  + 
Sbjct: 209 VDMGGRTFLVPGNFPLGCSATYLTLYQTSNKEEYDPLTGCLTWLNDFSEYYNEKLQAELN 268

Query: 282 SLRKNHPNVKIVYADYYHAFLWVLRYASLLEFDQTSLQKSCCGIGGDYDFNITQMCGLPG 341
            L K +P+V I+Y DY++A L + +  S   F    L  +CCG+GG Y+F +++ CG  G
Sbjct: 269 RLSKLYPHVNIIYGDYFNALLRLYQEPSKFGFMDRPL-PACCGLGGPYNFTLSKKCGSVG 328

Query: 342 VPVCPNPDERISWDGVHFTQKAYKYIAYWLIQ 358
           V  C +P + ++WDGVH T+ AYK+IA  L++
Sbjct: 329 VKYCSDPSKYVNWDGVHMTEAAYKWIADGLLK 358