Tan0014566 (gene) Snake gourd v1

Overview
NameTan0014566
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionAmino acid/polyamine transporter
LocationLG07: 10090953 .. 10092722 (-)
RNA-Seq ExpressionTan0014566
SyntenyTan0014566
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCGCTCAATACCCCAAACAACCCCATAAATAGTACCTTCCAATTTTTTCAATTTTTACCCTGGAAAGTTTAGTGGTCTCTGCAATTTACCCTATAATCCAACTTTAAACAGACATACACATAAATTCATACCACGTTGGTGCCACTCCGGTGAGATCCGGGAATCTGAGAGCGATTTGAAAAGAAAACCACTGAATCTGAATCAGAATCAGAATCCGAATCGAAGGTAACCAAACAAAGAAACGCCATGGGAGGTGAAATAGAAACGATTCATCAAAATCTTCTTCATCACGTTCATGATCAGCCATTAAATCAGCCCTCCAAGAAATTATCGCTTCTGCCCCTCATCTTCCTTATCTACTTCGAAGTCGCCGGCGGCCCCTTTGGCGAAGAGCTCGCCGTGAAGGCCGCCGGTCCCTTCCTCGCGATTGCCGGCTTCTTTATTTTCCCCTTCATTTGGAGCATCCCGGAGGCCCTCATCACCGCCGAGCTCGCCACCACCTTCCCCGGCAACGGCGGCTTCGTCATTTGGGCCTATGAAGCCTTCGGCCCTTTCTGGGGCTTCCTCATGGGCACTTGGAAGTTCTTCAGCGGCGTAATTAACTTAGCCTCATACCCAATTCTCTGTATCGATTATCTCAAACTTCTTTTGCCAATTCTCTCATCTGGATTCCCTCGTTTCGCCGCTTTTCTTGCCTCGACCTCCGCCCTTTCTTTCCTCAACTACACTGGTTTAACCGTCGTCGGATTCACCGCAATAGCGCTCGGAATCGTCTCGCTTTTCCCCTTTCTGATAATGTCTCTGTTCGCAATCCCCAATCTCGATTTCCGGCGATGGGTCGTCTCCGGCGAAAAGGGCACCGAGAAGAATTGGGGATTATTCTTCAACACCCTTTTCTGGAACCTGAATTTTTGGGACAATGCTAGTACGCTAGCCGGTGAAGTTGAGGAACCCCAAAAATCATACCCAAAAGCTCTGTTTTCAGCCGGGGTTTTGGCTTGTCTGGCTTACTTGATTCCTCTCATGGCGGTCATCGGAGCTCTGCCTCTGGATCAGAAAAATTGGGAGAGTGGGTATTTTGCAGATGCTGGTGAAATCCTTGTTGGGAAATGGCTCAAATACTGGGTTGAAATTGGGGCAGTTTTATCAATGATTGGGCTGTTCCAAGCTCAACTAAGCAGCTGCGCTTATCAGCTTGAAGGAATGGCAGATTTGGGATTTTTGCCAAAGCTTTTTGGGGAAAGATCTGTGAAGTTCAATACACCATGGGTTGGAATTCTGATTTCAACAGTGGTAGTGGTTGGAGTTTCTTCAATGGAGTTTGTGGACTTGATTTCTTCTGCAAATTTCTTGTACAGTTTAGGAATGGTGTTGGAATTTGGGTCATTTATATGGCTGAGGAAGAAGAAAGAAGGGTTAAAGAGGCCATTTAGAGTGCCATTAGGAGTGACTGGTTTGGTTGTAATGTGCTTGATTCCAAGTGGGTTTCTTTTGTATATAATGGCTTTGGCTAATAAAGCTGTTTGGTTGATCAGTGGTTTGATGACTCTTTTTGGAATGGGTTGCTATTTTTTGATCAAGTTCTTCAAACCAGAGATGGGTTTTGAACTTCATTCTCATCACAAGTTGCAAGATGAGCATGTGGGTGTGTAAAGTAAGTTTCTTTCATTCTATGCAAAAGAGAAAATGGTTGAATACTTATGAATATGCATTTATATTAAATTACAATTTAGCATCTTTATCTTCAATTATCTTTTCTAAACTTGGAG

mRNA sequence

CTCGCTCAATACCCCAAACAACCCCATAAATAGTACCTTCCAATTTTTTCAATTTTTACCCTGGAAAGTTTAGTGGTCTCTGCAATTTACCCTATAATCCAACTTTAAACAGACATACACATAAATTCATACCACGTTGGTGCCACTCCGGTGAGATCCGGGAATCTGAGAGCGATTTGAAAAGAAAACCACTGAATCTGAATCAGAATCAGAATCCGAATCGAAGGTAACCAAACAAAGAAACGCCATGGGAGGTGAAATAGAAACGATTCATCAAAATCTTCTTCATCACGTTCATGATCAGCCATTAAATCAGCCCTCCAAGAAATTATCGCTTCTGCCCCTCATCTTCCTTATCTACTTCGAAGTCGCCGGCGGCCCCTTTGGCGAAGAGCTCGCCGTGAAGGCCGCCGGTCCCTTCCTCGCGATTGCCGGCTTCTTTATTTTCCCCTTCATTTGGAGCATCCCGGAGGCCCTCATCACCGCCGAGCTCGCCACCACCTTCCCCGGCAACGGCGGCTTCGTCATTTGGGCCTATGAAGCCTTCGGCCCTTTCTGGGGCTTCCTCATGGGCACTTGGAAGTTCTTCAGCGGCGTAATTAACTTAGCCTCATACCCAATTCTCTGTATCGATTATCTCAAACTTCTTTTGCCAATTCTCTCATCTGGATTCCCTCGTTTCGCCGCTTTTCTTGCCTCGACCTCCGCCCTTTCTTTCCTCAACTACACTGGTTTAACCGTCGTCGGATTCACCGCAATAGCGCTCGGAATCGTCTCGCTTTTCCCCTTTCTGATAATGTCTCTGTTCGCAATCCCCAATCTCGATTTCCGGCGATGGGTCGTCTCCGGCGAAAAGGGCACCGAGAAGAATTGGGGATTATTCTTCAACACCCTTTTCTGGAACCTGAATTTTTGGGACAATGCTAGTACGCTAGCCGGTGAAGTTGAGGAACCCCAAAAATCATACCCAAAAGCTCTGTTTTCAGCCGGGGTTTTGGCTTGTCTGGCTTACTTGATTCCTCTCATGGCGGTCATCGGAGCTCTGCCTCTGGATCAGAAAAATTGGGAGAGTGGGTATTTTGCAGATGCTGGTGAAATCCTTGTTGGGAAATGGCTCAAATACTGGGTTGAAATTGGGGCAGTTTTATCAATGATTGGGCTGTTCCAAGCTCAACTAAGCAGCTGCGCTTATCAGCTTGAAGGAATGGCAGATTTGGGATTTTTGCCAAAGCTTTTTGGGGAAAGATCTGTGAAGTTCAATACACCATGGGTTGGAATTCTGATTTCAACAGTGGTAGTGGTTGGAGTTTCTTCAATGGAGTTTGTGGACTTGATTTCTTCTGCAAATTTCTTGTACAGTTTAGGAATGGTGTTGGAATTTGGGTCATTTATATGGCTGAGGAAGAAGAAAGAAGGGTTAAAGAGGCCATTTAGAGTGCCATTAGGAGTGACTGGTTTGGTTGTAATGTGCTTGATTCCAAGTGGGTTTCTTTTGTATATAATGGCTTTGGCTAATAAAGCTGTTTGGTTGATCAGTGGTTTGATGACTCTTTTTGGAATGGGTTGCTATTTTTTGATCAAGTTCTTCAAACCAGAGATGGGTTTTGAACTTCATTCTCATCACAAGTTGCAAGATGAGCATGTGGGTGTGTAAAGTAAGTTTCTTTCATTCTATGCAAAAGAGAAAATGGTTGAATACTTATGAATATGCATTTATATTAAATTACAATTTAGCATCTTTATCTTCAATTATCTTTTCTAAACTTGGAG

Coding sequence (CDS)

ATGGGAGGTGAAATAGAAACGATTCATCAAAATCTTCTTCATCACGTTCATGATCAGCCATTAAATCAGCCCTCCAAGAAATTATCGCTTCTGCCCCTCATCTTCCTTATCTACTTCGAAGTCGCCGGCGGCCCCTTTGGCGAAGAGCTCGCCGTGAAGGCCGCCGGTCCCTTCCTCGCGATTGCCGGCTTCTTTATTTTCCCCTTCATTTGGAGCATCCCGGAGGCCCTCATCACCGCCGAGCTCGCCACCACCTTCCCCGGCAACGGCGGCTTCGTCATTTGGGCCTATGAAGCCTTCGGCCCTTTCTGGGGCTTCCTCATGGGCACTTGGAAGTTCTTCAGCGGCGTAATTAACTTAGCCTCATACCCAATTCTCTGTATCGATTATCTCAAACTTCTTTTGCCAATTCTCTCATCTGGATTCCCTCGTTTCGCCGCTTTTCTTGCCTCGACCTCCGCCCTTTCTTTCCTCAACTACACTGGTTTAACCGTCGTCGGATTCACCGCAATAGCGCTCGGAATCGTCTCGCTTTTCCCCTTTCTGATAATGTCTCTGTTCGCAATCCCCAATCTCGATTTCCGGCGATGGGTCGTCTCCGGCGAAAAGGGCACCGAGAAGAATTGGGGATTATTCTTCAACACCCTTTTCTGGAACCTGAATTTTTGGGACAATGCTAGTACGCTAGCCGGTGAAGTTGAGGAACCCCAAAAATCATACCCAAAAGCTCTGTTTTCAGCCGGGGTTTTGGCTTGTCTGGCTTACTTGATTCCTCTCATGGCGGTCATCGGAGCTCTGCCTCTGGATCAGAAAAATTGGGAGAGTGGGTATTTTGCAGATGCTGGTGAAATCCTTGTTGGGAAATGGCTCAAATACTGGGTTGAAATTGGGGCAGTTTTATCAATGATTGGGCTGTTCCAAGCTCAACTAAGCAGCTGCGCTTATCAGCTTGAAGGAATGGCAGATTTGGGATTTTTGCCAAAGCTTTTTGGGGAAAGATCTGTGAAGTTCAATACACCATGGGTTGGAATTCTGATTTCAACAGTGGTAGTGGTTGGAGTTTCTTCAATGGAGTTTGTGGACTTGATTTCTTCTGCAAATTTCTTGTACAGTTTAGGAATGGTGTTGGAATTTGGGTCATTTATATGGCTGAGGAAGAAGAAAGAAGGGTTAAAGAGGCCATTTAGAGTGCCATTAGGAGTGACTGGTTTGGTTGTAATGTGCTTGATTCCAAGTGGGTTTCTTTTGTATATAATGGCTTTGGCTAATAAAGCTGTTTGGTTGATCAGTGGTTTGATGACTCTTTTTGGAATGGGTTGCTATTTTTTGATCAAGTTCTTCAAACCAGAGATGGGTTTTGAACTTCATTCTCATCACAAGTTGCAAGATGAGCATGTGGGTGTGTAA

Protein sequence

MGGEIETIHQNLLHHVHDQPLNQPSKKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWVVSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIPLMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFGMGCYFLIKFFKPEMGFELHSHHKLQDEHVGV
Homology
BLAST of Tan0014566 vs. ExPASy Swiss-Prot
Match: Q9LHN7 (Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g13620 PE=2 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 7.3e-163
Identity = 285/432 (65.97%), Postives = 342/432 (79.17%), Query Frame = 0

Query: 25  SKKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALITAELAT 84
           +KKL+L+PL+FLIYFEVAGGPFGEE AV+AAGP LAI GF IFPFIWSIPEALITAEL+T
Sbjct: 27  AKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLAILGFLIFPFIWSIPEALITAELST 86

Query: 85  TFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPILSSGFPR 144
            FPGNGGFVIWA+ AFG F G +MG+ KF SGVIN+AS+P+LC+ YL  L P+L SG+PR
Sbjct: 87  AFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINVASFPVLCVTYLDKLFPVLESGWPR 146

Query: 145 FAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWVVSGEKG 204
                AST  LSFLNYTGL +VG+ A+ LG+VSL PFL+MS  AIP +   RW   G K 
Sbjct: 147 NVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSPFLVMSAMAIPKIKPHRWGSLGTK- 206

Query: 205 TEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIPLMAVIG 264
            +K+W L+FNTLFWNLNFWDN STLAGEV+EPQK++P AL  A +  C+AYLIPL AV G
Sbjct: 207 -KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTFPLALLIAVIFTCVAYLIPLFAVTG 266

Query: 265 ALPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLEGMADLG 324
           A+ +DQ  WE+G+ A+A E++ GKWLK W+EIGAVLS IGLF+AQLSS AYQLEGMA+LG
Sbjct: 267 AVSVDQSRWENGFHAEAAEMIAGKWLKIWIEIGAVLSSIGLFEAQLSSSAYQLEGMAELG 326

Query: 325 FLPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEFGSFIWL 384
           FLPK FG RS  FNTPWVGILIS ++ +G+S M F D+ISSANFLY+LGM LEF SFIWL
Sbjct: 327 FLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFTDIISSANFLYTLGMFLEFASFIWL 386

Query: 385 RKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFGMGCYFLI 444
           R+K   LKRP+RVPL + GLVVMCLIPS FL+ I+  A K V+LI G+MT+  +G YFLI
Sbjct: 387 RRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILVFATKIVYLICGVMTIGAIGWYFLI 446

Query: 445 KFFKPEMGFELH 457
            +F+    FE +
Sbjct: 447 NYFRKTKIFEFN 456

BLAST of Tan0014566 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.5e-120
Identity = 214/413 (51.82%), Postives = 292/413 (70.70%), Query Frame = 0

Query: 26  KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALITAELATT 85
           KK+++LPL+FLI++EV+GGPFG E +VKAAGP LAI GF +FPFIWSIPEALITAE+ T 
Sbjct: 51  KKITVLPLVFLIFYEVSGGPFGIEDSVKAAGPLLAIVGFIVFPFIWSIPEALITAEMGTM 110

Query: 86  FPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPILSSGFPRF 145
           FP NGG+V+W   A GP+WGF  G  K+ SGVI+ A YPIL +DYLK  +PIL SG PR 
Sbjct: 111 FPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRV 170

Query: 146 AAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWVVSGEKGT 205
           AA L  T AL++LNY GL++VG  A+ LG+ S+ PF++MS  +IP L   RW+V  +K  
Sbjct: 171 AAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMK 230

Query: 206 EKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIPLMAVIGA 265
             NW L+ NTLFWNLN+WD+ STL GEVE P K+ P+ALF A +L   +Y+ P++   GA
Sbjct: 231 GVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGA 290

Query: 266 LPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLEGMADLGF 325
           + LDQK W  GYFAD G+++ G WL +W++  A  S +G+F A++SS ++QL GMA+ G 
Sbjct: 291 IALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGM 350

Query: 326 LPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEFGSFIWLR 385
           LP++F +RS ++ TPWVGIL S   V+ +S + F +++++ N LY  GMVLEF +F+ LR
Sbjct: 351 LPEVFAKRS-RYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLR 410

Query: 386 KKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFGM 439
            K     RPF++P+GV G V+MC+ P+  +  IMA  N  V L+S    + G+
Sbjct: 411 MKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGL 462

BLAST of Tan0014566 vs. ExPASy Swiss-Prot
Match: Q9LH39 (Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g19553 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 8.4e-119
Identity = 228/451 (50.55%), Postives = 310/451 (68.74%), Query Frame = 0

Query: 3   GEIETIHQNLLHHVHDQPLN--QPSKKLSLLPLIFLIYFEVAGGPFGEELAVKA-AGPFL 62
           GE ETI       V+D+  +  +PS KL+LLPL+FLI++EV+GGPFG E +VK+  GP L
Sbjct: 2   GEEETI-------VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLL 61

Query: 63  AIAGFFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVIN 122
           A+ GF IFP IWSIPEAL+TAELAT+FP NGG+V+W   AFGPFWGF  G WK+FSGV++
Sbjct: 62  ALLGFLIFPLIWSIPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMD 121

Query: 123 LASYPILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLF 182
            A YP+L +DYLK   P+L     R  A L  T +L++LNY GL +VGF+A+ L + SL 
Sbjct: 122 NALYPVLFLDYLKHSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLC 181

Query: 183 PFLIMSLFAIPNLDFRRWVVSGEKGTEK-NWGLFFNTLFWNLNFWDNASTLAGEVEEPQK 242
           PF++M+L A+PN+  +RW+      T+K NW  +FNT+FWNLN+WD ASTLAGEV+ P K
Sbjct: 182 PFVVMALLAVPNIRPKRWLF---VDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGK 241

Query: 243 SYPKALFSAGVLACLAYLIPLMAVIGALPLDQK-NWESGYFADAGEILVGKWLKYWVEIG 302
           ++PKALF A +L   +YLIPLMA  GAL       W  GYFA+ G ++ G WLK W++  
Sbjct: 242 TFPKALFGAVLLVMGSYLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAA 301

Query: 303 AVLSMIGLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSM 362
           A +S +GLF+A++SS A+QL GM+++G LP  F +RS K+ TP + IL S   V+ +S M
Sbjct: 302 AAMSNLGLFEAEMSSDAFQLLGMSEIGMLPAFFAQRS-KYGTPTISILCSATGVIFLSWM 361

Query: 363 EFVDLISSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLY 422
            F ++I   NFLY+LGM+LEF +F+ LR KK  L RP+RVPL   G+ ++CL PS  ++ 
Sbjct: 362 SFQEIIEFLNFLYALGMLLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVIL 421

Query: 423 IMALANKAVWLISGLMTLFGMGCYFLIKFFK 449
           +M LA    +LISG++ + G   Y  +   K
Sbjct: 422 VMVLAAPKTFLISGVIIVLGFCLYPFLTLVK 441

BLAST of Tan0014566 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 1.9e-115
Identity = 202/412 (49.03%), Postives = 290/412 (70.39%), Query Frame = 0

Query: 26  KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALITAELATT 85
           +K+S+LPL+FLI++EV+GGPFG E +V AAGP LA+ GF IFPFIWSIPEALITAE+ T 
Sbjct: 47  RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLGFVIFPFIWSIPEALITAEMGTM 106

Query: 86  FPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPILSSGFPRF 145
           +P NGG+V+W   A GPFWGF  G  K+ SGVI+ A YP+L +DYLK  +P L SG PR 
Sbjct: 107 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 166

Query: 146 AAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWVVSGEKGT 205
           A+ L  T  L++LNY GLT+VG+ A+ +G+ S+ PF +M L +IP L+  RW+V      
Sbjct: 167 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 226

Query: 206 EKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIPLMAVIGA 265
             NW L+ NTLFWNLN+WD+ STLAGEVE P  + PKALF   +L   +Y+ PL+A IGA
Sbjct: 227 --NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGA 286

Query: 266 LPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLEGMADLGF 325
           +PL+++ W  GYF+D  + L G WL++WV+  A  S +G+F A++SS ++QL GMA+ G 
Sbjct: 287 IPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGM 346

Query: 326 LPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEFGSFIWLR 385
           LP+ F +RS ++ TP +GIL S   VV +S + F +++++ N LY +GM+LEF +F+ +R
Sbjct: 347 LPEFFAKRS-RYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMR 406

Query: 386 KKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFG 438
            K     RP+++P+G TG ++MC+ P+  +  ++AL++  V  +S +M + G
Sbjct: 407 MKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIG 455

BLAST of Tan0014566 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.0e-112
Identity = 211/428 (49.30%), Postives = 283/428 (66.12%), Query Frame = 0

Query: 3   GEIETIHQNLLHHVHDQPLNQPSKKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIA 62
           GE  T ++ L       P+   ++ +S++PLIFLI++EV+GGPFG E +V AAGP LAI 
Sbjct: 58  GECGTEYRGLPDGDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVGAAGPLLAII 117

Query: 63  GFFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLAS 122
           GF + P IWSIPEALITAEL   FP NGG+V+W   A GP+WGF  G  K+ SGVI+ A 
Sbjct: 118 GFLVLPVIWSIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNAL 177

Query: 123 YPILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFL 182
           YP+L +DYLK  +P L  G PR  A +  T+ L+ LNY GLTVVG+ AI LG+ SL PF 
Sbjct: 178 YPVLFLDYLKSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFF 237

Query: 183 IMSLFAIPNLDFRRWVVSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPK 242
           +M L A+P L   RW+V      +  W L+ NTLFWNLN+WD+ STLAGEV+ P K+ PK
Sbjct: 238 VMGLIALPKLRPARWLVIDLHNVD--WNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPK 297

Query: 243 ALFSAGVLACLAYLIPLMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSM 302
           ALF A +   +AYL PL+A  GA+PLD+  W  GYFAD  ++L G WL +WV+  A LS 
Sbjct: 298 ALFYAVIFVVVAYLYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSN 357

Query: 303 IGLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDL 362
           +G+F A++SS +YQL GMA+ G LP  F  RS ++ TP  GIL S   V+ +S M F ++
Sbjct: 358 MGMFVAEMSSDSYQLLGMAERGMLPSFFAARS-RYGTPLAGILFSASGVLLLSMMSFQEI 417

Query: 363 ISSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALA 422
           +++ NFLY  GM+LEF +FI  R ++    RP+RVPLG  G V M + P+  +  ++AL+
Sbjct: 418 VAAENFLYCFGMLLEFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALS 477

Query: 423 NKAVWLIS 431
              V ++S
Sbjct: 478 TLKVAVVS 482

BLAST of Tan0014566 vs. NCBI nr
Match: RXH85337.1 (hypothetical protein DVH24_042105 [Malus domestica])

HSP 1 Score: 646.7 bits (1667), Expect = 1.5e-181
Identity = 316/460 (68.70%), Postives = 377/460 (81.96%), Query Frame = 0

Query: 8   IHQNLL--HHVHDQPLNQPS--KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAG 67
           I Q+LL       QP   P   KKL+LLPL+FLIYFEV+GGP+GEE AV AAGP  AI G
Sbjct: 12  ISQSLLEQQEAESQPQISPQNPKKLALLPLVFLIYFEVSGGPYGEESAVGAAGPLFAILG 71

Query: 68  FFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASY 127
           F IFPFIWSIPEAL+TAEL+TTFPGNGGFVIWA++AFGPFWG LMG+WKF SGVINLASY
Sbjct: 72  FLIFPFIWSIPEALVTAELSTTFPGNGGFVIWAHQAFGPFWGSLMGSWKFLSGVINLASY 131

Query: 128 PILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLI 187
           PILC+DYLKL++PI +SG PRF A   ST  LSFLNY+GL++VG+TA+ LGIVSL PF++
Sbjct: 132 PILCVDYLKLVIPIFASGLPRFFAIFISTLVLSFLNYSGLSIVGYTAVGLGIVSLCPFIL 191

Query: 188 MSLFAIPNLDFRRWVVSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKA 247
           MSL AIP +   RW+  GEKG +K+W LF NTLFWNLNFWDNASTLAGEVE+PQK +PKA
Sbjct: 192 MSLIAIPKIHPSRWISLGEKGVKKDWTLFINTLFWNLNFWDNASTLAGEVEQPQKLFPKA 251

Query: 248 LFSAGVLACLAYLIPLMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMI 307
           LFSAG+L CL Y+IPL+A  GA+PLDQ++W  GY A AGE++ GKWLK+W+EIGAVLS+I
Sbjct: 252 LFSAGILTCLGYIIPLLAATGAIPLDQESWVDGYLATAGEMIAGKWLKFWIEIGAVLSII 311

Query: 308 GLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLI 367
           GLF+AQLSSCAYQL GMA+LGFLPK+FG RS  FNTPW+GILISTV+ + VS ++F D+I
Sbjct: 312 GLFEAQLSSCAYQLLGMANLGFLPKIFGARSKWFNTPWLGILISTVIALAVSYLDFTDII 371

Query: 368 SSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALAN 427
           SSANFLYSLGM+LEF SFIWLR K   LKRPF+VP+G+ GLVVMC IPS FL+Y++A+  
Sbjct: 372 SSANFLYSLGMLLEFASFIWLRVKFPALKRPFKVPMGLLGLVVMCFIPSVFLVYVLAVGT 431

Query: 428 KAVWLISGLMTLFGMGCYFLIKFFKPEMGFELHSHHKLQD 464
           KAV+L+S LMTLFG+G YFL+   K +M FE +   K  D
Sbjct: 432 KAVYLVSALMTLFGVGWYFLMNLSKSKMWFEFNLGEKKLD 471

BLAST of Tan0014566 vs. NCBI nr
Match: TQE02057.1 (hypothetical protein C1H46_012376 [Malus baccata])

HSP 1 Score: 646.7 bits (1667), Expect = 1.5e-181
Identity = 316/460 (68.70%), Postives = 377/460 (81.96%), Query Frame = 0

Query: 8   IHQNLL--HHVHDQPLNQPS--KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAG 67
           I Q+LL       QP   P   KKL+LLPL+FLIYFEV+GGP+GEE AV AAGP  AI G
Sbjct: 12  ISQSLLEQQEAESQPQISPQNPKKLALLPLVFLIYFEVSGGPYGEESAVGAAGPLFAILG 71

Query: 68  FFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASY 127
           F IFPFIWSIPEAL+TAEL+TTFPGNGGFVIWA++AFGPFWG LMG+WKF SGVINLASY
Sbjct: 72  FLIFPFIWSIPEALVTAELSTTFPGNGGFVIWAHQAFGPFWGSLMGSWKFLSGVINLASY 131

Query: 128 PILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLI 187
           PILC+DYLKL++PI +SG PRF A   ST  LSFLNY+GL++VG+TA+ LGIVSL PF++
Sbjct: 132 PILCVDYLKLVIPIFASGLPRFFAIFISTLVLSFLNYSGLSIVGYTAVGLGIVSLCPFIL 191

Query: 188 MSLFAIPNLDFRRWVVSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKA 247
           MSL AIP +   RW+  GEKG +K+W LF NTLFWNLNFWDNASTLAGEVE+PQK +PKA
Sbjct: 192 MSLIAIPKIHPSRWISLGEKGVKKDWTLFINTLFWNLNFWDNASTLAGEVEQPQKLFPKA 251

Query: 248 LFSAGVLACLAYLIPLMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMI 307
           LFSAG+L CL Y+IPL+A  GA+PLDQ++W  GY A AGE++ GKWLK+W+EIGAVLS+I
Sbjct: 252 LFSAGILTCLGYIIPLLAATGAIPLDQESWVDGYLATAGEMIAGKWLKFWIEIGAVLSII 311

Query: 308 GLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLI 367
           GLF+AQLSSCAYQL GMA+LGFLPK+FG RS  FNTPW+GILISTV+ + VS ++F D+I
Sbjct: 312 GLFEAQLSSCAYQLLGMANLGFLPKIFGARSKWFNTPWLGILISTVIALAVSYLDFTDII 371

Query: 368 SSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALAN 427
           SSANFLYSLGM+LEF SFIWLR K   LKRPF+VP+G+ GLVVMC IPS FL+Y++A+  
Sbjct: 372 SSANFLYSLGMLLEFASFIWLRVKFPALKRPFKVPMGLLGLVVMCFIPSVFLVYVLAVGT 431

Query: 428 KAVWLISGLMTLFGMGCYFLIKFFKPEMGFELHSHHKLQD 464
           KAV+L+S LMTLFG+G YFL+   K +M FE +   K  D
Sbjct: 432 KAVYLVSALMTLFGVGWYFLMNLSKSKMWFEFNLGEKKLD 471

BLAST of Tan0014566 vs. NCBI nr
Match: KAF3949085.1 (hypothetical protein CMV_024993 [Castanea mollissima])

HSP 1 Score: 646.4 bits (1666), Expect = 2.0e-181
Identity = 311/440 (70.68%), Postives = 375/440 (85.23%), Query Frame = 0

Query: 27  KLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALITAELATTF 86
           KL+L+PL+FLIYFEVAGGP+GEE AV AAGP  AI GF IFPFIWSIPEAL+TAELATTF
Sbjct: 35  KLALIPLVFLIYFEVAGGPYGEEAAVGAAGPLFAILGFLIFPFIWSIPEALVTAELATTF 94

Query: 87  PGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPILSSGFPRFA 146
           PGNGGFVIWA++AFGPFWG LMG+WKFFSGVINLASYP+LCIDYLKL+ PI S+G PR+ 
Sbjct: 95  PGNGGFVIWAHQAFGPFWGSLMGSWKFFSGVINLASYPVLCIDYLKLVFPIFSTGVPRYL 154

Query: 147 AFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWVVSGEKGTE 206
           A   ST  LSFLNYTGLT+VG+TA+ LG+VSL PF++M+L AIP ++  RW+  G++G E
Sbjct: 155 AIFVSTLVLSFLNYTGLTIVGYTAVTLGVVSLLPFVVMTLVAIPKIEPSRWISLGQEGVE 214

Query: 207 KNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIPLMAVIGAL 266
           K+W LFFNTLFWNLNFWDNASTLAGEVEEPQK++PKALFSAG+L CLAYLIPL+A IGA+
Sbjct: 215 KDWTLFFNTLFWNLNFWDNASTLAGEVEEPQKTFPKALFSAGLLTCLAYLIPLLAAIGAI 274

Query: 267 PLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLEGMADLGFL 326
           PLDQ++W  GYFADA EI+ GKWLK W+EIGAVLS+IGL++AQLSSCAYQL GMA+LGFL
Sbjct: 275 PLDQEDWVDGYFADAAEIIAGKWLKVWIEIGAVLSVIGLYEAQLSSCAYQLLGMANLGFL 334

Query: 327 PKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEFGSFIWLRK 386
           P+ FG RS  FNTPWVGIL+ST++ + V+ M FVD+ISS NFLYSLGM+LEF SF+WLR+
Sbjct: 335 PQFFGVRSTWFNTPWVGILLSTLITLAVAYMSFVDIISSVNFLYSLGMLLEFASFLWLRR 394

Query: 387 KKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFGMGCYFLIKF 446
           K   +KRPF VP+G+  LVVMCLIPSGFLLY+MA+A K V+L++ L+TL G+  YFL+ F
Sbjct: 395 KLPTVKRPFVVPMGLPWLVVMCLIPSGFLLYVMAVATKTVYLVAALLTLAGIAWYFLMNF 454

Query: 447 FKPEM--GFELHSHHKLQDE 465
            K +M  GF  ++  KL+D+
Sbjct: 455 CKSKMWLGFN-NAGEKLEDQ 473

BLAST of Tan0014566 vs. NCBI nr
Match: XP_042957702.1 (probable polyamine transporter At3g13620 [Carya illinoinensis] >KAG6630127.1 hypothetical protein CIPAW_14G134400 [Carya illinoinensis] >KAG6679524.1 hypothetical protein I3842_14G136500 [Carya illinoinensis] >KAG7948176.1 hypothetical protein I3843_14G135100 [Carya illinoinensis])

HSP 1 Score: 646.0 bits (1665), Expect = 2.6e-181
Identity = 307/447 (68.68%), Postives = 375/447 (83.89%), Query Frame = 0

Query: 19  QPLNQPSKKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALI 78
           +P ++  KKL+L+PL+FLIYFEVAGGP+GEE AV AAGP  AI GF IFPF+WSIPEA++
Sbjct: 18  EPSSKSPKKLALIPLVFLIYFEVAGGPYGEESAVGAAGPLFAILGFLIFPFVWSIPEAVV 77

Query: 79  TAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPIL 138
           TAELATTFPGNGG+VIWA  AFGPFWG LMG+WKFFSGVINLASYP+LC+DYLKL+LPI 
Sbjct: 78  TAELATTFPGNGGYVIWANHAFGPFWGSLMGSWKFFSGVINLASYPVLCVDYLKLVLPIF 137

Query: 139 SSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWV 198
           SSG PR+ A + ST  LSFLNYTGLT+VG+TA+ LG+VSL PF++MSL AIP +D  RW+
Sbjct: 138 SSGLPRYFAVVLSTLVLSFLNYTGLTIVGYTAVTLGVVSLLPFIVMSLVAIPKIDPSRWI 197

Query: 199 VSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIP 258
             G++G +K+W LFFNTLFWNLNFWDNASTLAGEVE+PQK+YPKALFSAG+L CLAYLIP
Sbjct: 198 SLGQEGVQKDWTLFFNTLFWNLNFWDNASTLAGEVEQPQKTYPKALFSAGLLTCLAYLIP 257

Query: 259 LMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLE 318
           L+A  GA+PL Q++W  GYFADA EI+ GKWLK W+EIGAVLS+IGL++AQLSSCAYQL 
Sbjct: 258 LLAATGAMPLHQEDWVDGYFADAAEIIAGKWLKVWIEIGAVLSVIGLYEAQLSSCAYQLL 317

Query: 319 GMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEF 378
           GMADL FLP++FG RS  F TPWVGIL+ST++ + VS M FV++ISS NFLYSLGM+LEF
Sbjct: 318 GMADLAFLPRIFGARSTWFRTPWVGILLSTLITLAVSFMSFVNIISSVNFLYSLGMLLEF 377

Query: 379 GSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFGM 438
            SF+WLR+K   +KRPF+VPLG+ GLVVMCLIPSGFL+Y+MA+A   V+L+S L+TL G+
Sbjct: 378 SSFLWLRRKLPAVKRPFKVPLGLPGLVVMCLIPSGFLVYVMAVATNIVFLVSALLTLAGI 437

Query: 439 GCYFLIKFFKPEMGFEL-HSHHKLQDE 465
             YF ++F K     E  ++  K++DE
Sbjct: 438 AWYFFMEFCKARTWLEFNNAGEKIEDE 464

BLAST of Tan0014566 vs. NCBI nr
Match: PQP93813.1 (putative polyamine transporter [Prunus yedoensis var. nudiflora])

HSP 1 Score: 644.4 bits (1661), Expect = 7.4e-181
Identity = 315/470 (67.02%), Postives = 377/470 (80.21%), Query Frame = 0

Query: 4   EIETIHQNLLHHVHDQPLNQPS------KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGP 63
           E+ T  Q+LL    +Q  +QP       KKL+ LPL+FLIYFEV+GGP+GEE AV AAGP
Sbjct: 8   ELSTSSQSLLE--QEQTESQPKITIKNPKKLAFLPLVFLIYFEVSGGPYGEESAVGAAGP 67

Query: 64  FLAIAGFFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGV 123
             AI GF IFPFIWSIPEAL+TAELAT +PGNGGFVIWA++AFGPFWG LMG+WKF SGV
Sbjct: 68  LFAILGFLIFPFIWSIPEALVTAELATAYPGNGGFVIWAHQAFGPFWGSLMGSWKFLSGV 127

Query: 124 INLASYPILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVS 183
           INLASYPILC+DYLKL++PI SSG PRF A   ST  LSFLNY+GL++VG+TA+ LGIVS
Sbjct: 128 INLASYPILCVDYLKLVIPIFSSGLPRFVAVFVSTLVLSFLNYSGLSIVGYTAVGLGIVS 187

Query: 184 LFPFLIMSLFAIPNLDFRRWVVSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQ 243
           L PF+IMSL AIP +D  RW+  G+KG +K+W LF NTLFWNLNFWDNASTLAGEV+EPQ
Sbjct: 188 LCPFIIMSLVAIPKIDPSRWISLGQKGVKKDWTLFINTLFWNLNFWDNASTLAGEVDEPQ 247

Query: 244 KSYPKALFSAGVLACLAYLIPLMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIG 303
           K YPKALFSAG+LACL Y+IPL+A  GA+PLDQ++W  GY A AGE++ GKWLK+W+EIG
Sbjct: 248 KLYPKALFSAGILACLGYIIPLLAATGAIPLDQEDWVDGYLASAGEMIAGKWLKFWIEIG 307

Query: 304 AVLSMIGLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSM 363
           AVLS+IGLF+AQLSSCAYQL GMADLG LP +FG RS  FNTPW+GILISTV+ + VS +
Sbjct: 308 AVLSIIGLFEAQLSSCAYQLLGMADLGILPMIFGARSKWFNTPWLGILISTVIALSVSYL 367

Query: 364 EFVDLISSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLY 423
           +F D+ISSANFLYSLGM+LEF SF+WLR K   LKRPF VP+G+ GLVVMCLIPSGFL+Y
Sbjct: 368 DFTDIISSANFLYSLGMLLEFASFLWLRVKFPALKRPFEVPMGLPGLVVMCLIPSGFLVY 427

Query: 424 IMALANKAVWLISGLMTLFGMGCYFLIKFFKPEMGFELHSHHKLQDEHVG 468
           ++A+A KAV+L+S LMTL G+  Y  +   K +M F+     +  D   G
Sbjct: 428 VLAVATKAVYLVSALMTLSGVAWYLFMNLSKSKMWFDFKMEEEKLDNEEG 475

BLAST of Tan0014566 vs. ExPASy TrEMBL
Match: A0A498IT94 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_042105 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 7.2e-182
Identity = 316/460 (68.70%), Postives = 377/460 (81.96%), Query Frame = 0

Query: 8   IHQNLL--HHVHDQPLNQPS--KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAG 67
           I Q+LL       QP   P   KKL+LLPL+FLIYFEV+GGP+GEE AV AAGP  AI G
Sbjct: 12  ISQSLLEQQEAESQPQISPQNPKKLALLPLVFLIYFEVSGGPYGEESAVGAAGPLFAILG 71

Query: 68  FFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASY 127
           F IFPFIWSIPEAL+TAEL+TTFPGNGGFVIWA++AFGPFWG LMG+WKF SGVINLASY
Sbjct: 72  FLIFPFIWSIPEALVTAELSTTFPGNGGFVIWAHQAFGPFWGSLMGSWKFLSGVINLASY 131

Query: 128 PILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLI 187
           PILC+DYLKL++PI +SG PRF A   ST  LSFLNY+GL++VG+TA+ LGIVSL PF++
Sbjct: 132 PILCVDYLKLVIPIFASGLPRFFAIFISTLVLSFLNYSGLSIVGYTAVGLGIVSLCPFIL 191

Query: 188 MSLFAIPNLDFRRWVVSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKA 247
           MSL AIP +   RW+  GEKG +K+W LF NTLFWNLNFWDNASTLAGEVE+PQK +PKA
Sbjct: 192 MSLIAIPKIHPSRWISLGEKGVKKDWTLFINTLFWNLNFWDNASTLAGEVEQPQKLFPKA 251

Query: 248 LFSAGVLACLAYLIPLMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMI 307
           LFSAG+L CL Y+IPL+A  GA+PLDQ++W  GY A AGE++ GKWLK+W+EIGAVLS+I
Sbjct: 252 LFSAGILTCLGYIIPLLAATGAIPLDQESWVDGYLATAGEMIAGKWLKFWIEIGAVLSII 311

Query: 308 GLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLI 367
           GLF+AQLSSCAYQL GMA+LGFLPK+FG RS  FNTPW+GILISTV+ + VS ++F D+I
Sbjct: 312 GLFEAQLSSCAYQLLGMANLGFLPKIFGARSKWFNTPWLGILISTVIALAVSYLDFTDII 371

Query: 368 SSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALAN 427
           SSANFLYSLGM+LEF SFIWLR K   LKRPF+VP+G+ GLVVMC IPS FL+Y++A+  
Sbjct: 372 SSANFLYSLGMLLEFASFIWLRVKFPALKRPFKVPMGLLGLVVMCFIPSVFLVYVLAVGT 431

Query: 428 KAVWLISGLMTLFGMGCYFLIKFFKPEMGFELHSHHKLQD 464
           KAV+L+S LMTLFG+G YFL+   K +M FE +   K  D
Sbjct: 432 KAVYLVSALMTLFGVGWYFLMNLSKSKMWFEFNLGEKKLD 471

BLAST of Tan0014566 vs. ExPASy TrEMBL
Match: A0A540MTD0 (Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_012376 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 7.2e-182
Identity = 316/460 (68.70%), Postives = 377/460 (81.96%), Query Frame = 0

Query: 8   IHQNLL--HHVHDQPLNQPS--KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAG 67
           I Q+LL       QP   P   KKL+LLPL+FLIYFEV+GGP+GEE AV AAGP  AI G
Sbjct: 12  ISQSLLEQQEAESQPQISPQNPKKLALLPLVFLIYFEVSGGPYGEESAVGAAGPLFAILG 71

Query: 68  FFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASY 127
           F IFPFIWSIPEAL+TAEL+TTFPGNGGFVIWA++AFGPFWG LMG+WKF SGVINLASY
Sbjct: 72  FLIFPFIWSIPEALVTAELSTTFPGNGGFVIWAHQAFGPFWGSLMGSWKFLSGVINLASY 131

Query: 128 PILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLI 187
           PILC+DYLKL++PI +SG PRF A   ST  LSFLNY+GL++VG+TA+ LGIVSL PF++
Sbjct: 132 PILCVDYLKLVIPIFASGLPRFFAIFISTLVLSFLNYSGLSIVGYTAVGLGIVSLCPFIL 191

Query: 188 MSLFAIPNLDFRRWVVSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKA 247
           MSL AIP +   RW+  GEKG +K+W LF NTLFWNLNFWDNASTLAGEVE+PQK +PKA
Sbjct: 192 MSLIAIPKIHPSRWISLGEKGVKKDWTLFINTLFWNLNFWDNASTLAGEVEQPQKLFPKA 251

Query: 248 LFSAGVLACLAYLIPLMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMI 307
           LFSAG+L CL Y+IPL+A  GA+PLDQ++W  GY A AGE++ GKWLK+W+EIGAVLS+I
Sbjct: 252 LFSAGILTCLGYIIPLLAATGAIPLDQESWVDGYLATAGEMIAGKWLKFWIEIGAVLSII 311

Query: 308 GLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLI 367
           GLF+AQLSSCAYQL GMA+LGFLPK+FG RS  FNTPW+GILISTV+ + VS ++F D+I
Sbjct: 312 GLFEAQLSSCAYQLLGMANLGFLPKIFGARSKWFNTPWLGILISTVIALAVSYLDFTDII 371

Query: 368 SSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALAN 427
           SSANFLYSLGM+LEF SFIWLR K   LKRPF+VP+G+ GLVVMC IPS FL+Y++A+  
Sbjct: 372 SSANFLYSLGMLLEFASFIWLRVKFPALKRPFKVPMGLLGLVVMCFIPSVFLVYVLAVGT 431

Query: 428 KAVWLISGLMTLFGMGCYFLIKFFKPEMGFELHSHHKLQD 464
           KAV+L+S LMTLFG+G YFL+   K +M FE +   K  D
Sbjct: 432 KAVYLVSALMTLFGVGWYFLMNLSKSKMWFEFNLGEKKLD 471

BLAST of Tan0014566 vs. ExPASy TrEMBL
Match: A0A314XR84 (Putative polyamine transporter OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_10023 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 3.6e-181
Identity = 315/470 (67.02%), Postives = 377/470 (80.21%), Query Frame = 0

Query: 4   EIETIHQNLLHHVHDQPLNQPS------KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGP 63
           E+ T  Q+LL    +Q  +QP       KKL+ LPL+FLIYFEV+GGP+GEE AV AAGP
Sbjct: 8   ELSTSSQSLLE--QEQTESQPKITIKNPKKLAFLPLVFLIYFEVSGGPYGEESAVGAAGP 67

Query: 64  FLAIAGFFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGV 123
             AI GF IFPFIWSIPEAL+TAELAT +PGNGGFVIWA++AFGPFWG LMG+WKF SGV
Sbjct: 68  LFAILGFLIFPFIWSIPEALVTAELATAYPGNGGFVIWAHQAFGPFWGSLMGSWKFLSGV 127

Query: 124 INLASYPILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVS 183
           INLASYPILC+DYLKL++PI SSG PRF A   ST  LSFLNY+GL++VG+TA+ LGIVS
Sbjct: 128 INLASYPILCVDYLKLVIPIFSSGLPRFVAVFVSTLVLSFLNYSGLSIVGYTAVGLGIVS 187

Query: 184 LFPFLIMSLFAIPNLDFRRWVVSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQ 243
           L PF+IMSL AIP +D  RW+  G+KG +K+W LF NTLFWNLNFWDNASTLAGEV+EPQ
Sbjct: 188 LCPFIIMSLVAIPKIDPSRWISLGQKGVKKDWTLFINTLFWNLNFWDNASTLAGEVDEPQ 247

Query: 244 KSYPKALFSAGVLACLAYLIPLMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIG 303
           K YPKALFSAG+LACL Y+IPL+A  GA+PLDQ++W  GY A AGE++ GKWLK+W+EIG
Sbjct: 248 KLYPKALFSAGILACLGYIIPLLAATGAIPLDQEDWVDGYLASAGEMIAGKWLKFWIEIG 307

Query: 304 AVLSMIGLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSM 363
           AVLS+IGLF+AQLSSCAYQL GMADLG LP +FG RS  FNTPW+GILISTV+ + VS +
Sbjct: 308 AVLSIIGLFEAQLSSCAYQLLGMADLGILPMIFGARSKWFNTPWLGILISTVIALSVSYL 367

Query: 364 EFVDLISSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLY 423
           +F D+ISSANFLYSLGM+LEF SF+WLR K   LKRPF VP+G+ GLVVMCLIPSGFL+Y
Sbjct: 368 DFTDIISSANFLYSLGMLLEFASFLWLRVKFPALKRPFEVPMGLPGLVVMCLIPSGFLVY 427

Query: 424 IMALANKAVWLISGLMTLFGMGCYFLIKFFKPEMGFELHSHHKLQDEHVG 468
           ++A+A KAV+L+S LMTL G+  Y  +   K +M F+     +  D   G
Sbjct: 428 VLAVATKAVYLVSALMTLSGVAWYLFMNLSKSKMWFDFKMEEEKLDNEEG 475

BLAST of Tan0014566 vs. ExPASy TrEMBL
Match: A0A2I4EDQ5 (probable polyamine transporter At3g13620 OS=Juglans regia OX=51240 GN=LOC108988664 PE=3 SV=2)

HSP 1 Score: 644.4 bits (1661), Expect = 3.6e-181
Identity = 306/447 (68.46%), Postives = 374/447 (83.67%), Query Frame = 0

Query: 19  QPLNQPSKKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALI 78
           +P ++  KKL+L+PL+FLIYFEVAGGP+GEE AV AAGP  AI GF IFPFIWSIPEA++
Sbjct: 18  EPSSKSPKKLALIPLVFLIYFEVAGGPYGEESAVGAAGPLFAILGFLIFPFIWSIPEAVV 77

Query: 79  TAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPIL 138
           TAELATTFPGNGG+VIWA  AFGPFWG LMG+WKFFSGVINLASYP+LC+DYLKL+LPI 
Sbjct: 78  TAELATTFPGNGGYVIWANHAFGPFWGSLMGSWKFFSGVINLASYPVLCVDYLKLVLPIF 137

Query: 139 SSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWV 198
           SSG PR+ A + ST  LSFLNYTGLT+VG+TA+ LG+VSL PF++MSL AIP +D  RW+
Sbjct: 138 SSGLPRYFAVVVSTLVLSFLNYTGLTIVGYTAVTLGVVSLLPFIVMSLVAIPKIDPSRWI 197

Query: 199 VSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIP 258
             G+ G +K+W LFFNTLFWNLNFWDNASTLAGEVE+PQK+YPKALFSAG+L CLAYLIP
Sbjct: 198 SLGQDGVKKDWTLFFNTLFWNLNFWDNASTLAGEVEQPQKTYPKALFSAGLLTCLAYLIP 257

Query: 259 LMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLE 318
           L+A  G++PL Q++W  GYFADA EI+ GKWLK W+EIGAVLS+IGL++AQLSSCAYQL 
Sbjct: 258 LLAATGSIPLHQEDWVDGYFADAAEIIAGKWLKVWIEIGAVLSVIGLYEAQLSSCAYQLL 317

Query: 319 GMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEF 378
           GMADL FLP++FG RS  F TPWVGI +ST++ + VS M FV++ISS NFLYSLGM+LEF
Sbjct: 318 GMADLAFLPRIFGARSTWFRTPWVGIFLSTLITLAVSFMSFVNIISSVNFLYSLGMLLEF 377

Query: 379 GSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFGM 438
            SF+WLR+K   +KRPF+VPLG+ GLVVMCLIPSGFL+Y+MA+A   V+L+S L+TL G+
Sbjct: 378 SSFLWLRRKLPAVKRPFKVPLGLPGLVVMCLIPSGFLVYVMAVATSTVFLVSALLTLAGI 437

Query: 439 GCYFLIKFFKPEMGFEL-HSHHKLQDE 465
             YF ++F K  +  E  ++  K++DE
Sbjct: 438 AWYFFMEFCKSRLWLEFNNAGEKIEDE 464

BLAST of Tan0014566 vs. ExPASy TrEMBL
Match: A0A5E4F7W1 (PREDICTED: probable polyamine transporter OS=Prunus dulcis OX=3755 GN=ALMOND_2B020685 PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 1.0e-180
Identity = 315/468 (67.31%), Postives = 377/468 (80.56%), Query Frame = 0

Query: 4   EIETIHQNLLHHVHDQPLNQPS------KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGP 63
           E  T  Q+L+    +Q  +QP       KKL+ LPL+FLIYFEV+GGP+GEE AV AAGP
Sbjct: 8   EFSTSSQSLIE--QEQTESQPKITTKNPKKLAFLPLVFLIYFEVSGGPYGEESAVGAAGP 67

Query: 64  FLAIAGFFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGV 123
             AI GF IFPFIWSIPEAL+TAELAT +PGNGGFVIWA++AFGPFWG LMG+WKF SGV
Sbjct: 68  LFAILGFLIFPFIWSIPEALVTAELATAYPGNGGFVIWAHQAFGPFWGSLMGSWKFLSGV 127

Query: 124 INLASYPILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVS 183
           INLASYPILC+DYLKL++PI SSG PRF A   ST  LSFLNY+GL++VG+TA+ LGIVS
Sbjct: 128 INLASYPILCVDYLKLVIPIFSSGLPRFVAVFVSTLVLSFLNYSGLSIVGYTAVGLGIVS 187

Query: 184 LFPFLIMSLFAIPNLDFRRWVVSGEKGTEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQ 243
           L PF+IMSL AIP +D  RW+  G+KG +++W LF NTLFWNLNFWDNASTLAGEVEEPQ
Sbjct: 188 LCPFIIMSLVAIPKIDPSRWISLGQKGVKRDWTLFINTLFWNLNFWDNASTLAGEVEEPQ 247

Query: 244 KSYPKALFSAGVLACLAYLIPLMAVIGALPLDQKNWESGYFADAGEILVGKWLKYWVEIG 303
           K YPKALFSAG+L CL Y+IPL+A  GA+PLDQ++W  GY A AGE++ GKWLK+W+EIG
Sbjct: 248 KLYPKALFSAGILTCLGYVIPLLAATGAIPLDQEDWVDGYLASAGEMIAGKWLKFWIEIG 307

Query: 304 AVLSMIGLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSM 363
           AVLS+IGLF+AQLSSCAYQL GMADLG LP +FG RS  FNTPW+GILISTV+ + VS +
Sbjct: 308 AVLSIIGLFEAQLSSCAYQLLGMADLGILPMIFGARSKWFNTPWLGILISTVIALSVSYL 367

Query: 364 EFVDLISSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLY 423
           +F D+ISSANFLYSLGM+LEF SF+WLR K   LKRPF VP+G+ GLVVMCLIPSGFL+Y
Sbjct: 368 DFTDIISSANFLYSLGMLLEFASFLWLRVKFPALKRPFEVPMGLPGLVVMCLIPSGFLVY 427

Query: 424 IMALANKAVWLISGLMTLFGMGCYFLIKFFKPEMGFELH-SHHKLQDE 465
           ++A+A KAV+L+S LMTLFG+  Y  +   K +M F+      KL +E
Sbjct: 428 VLAVATKAVYLVSALMTLFGVAWYLFMNLSKSKMWFDFKMEEEKLDNE 473

BLAST of Tan0014566 vs. TAIR 10
Match: AT3G13620.1 (Amino acid permease family protein )

HSP 1 Score: 575.1 bits (1481), Expect = 5.2e-164
Identity = 285/432 (65.97%), Postives = 342/432 (79.17%), Query Frame = 0

Query: 25  SKKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALITAELAT 84
           +KKL+L+PL+FLIYFEVAGGPFGEE AV+AAGP LAI GF IFPFIWSIPEALITAEL+T
Sbjct: 27  AKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLAILGFLIFPFIWSIPEALITAELST 86

Query: 85  TFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPILSSGFPR 144
            FPGNGGFVIWA+ AFG F G +MG+ KF SGVIN+AS+P+LC+ YL  L P+L SG+PR
Sbjct: 87  AFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINVASFPVLCVTYLDKLFPVLESGWPR 146

Query: 145 FAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWVVSGEKG 204
                AST  LSFLNYTGL +VG+ A+ LG+VSL PFL+MS  AIP +   RW   G K 
Sbjct: 147 NVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSPFLVMSAMAIPKIKPHRWGSLGTK- 206

Query: 205 TEKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIPLMAVIG 264
            +K+W L+FNTLFWNLNFWDN STLAGEV+EPQK++P AL  A +  C+AYLIPL AV G
Sbjct: 207 -KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTFPLALLIAVIFTCVAYLIPLFAVTG 266

Query: 265 ALPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLEGMADLG 324
           A+ +DQ  WE+G+ A+A E++ GKWLK W+EIGAVLS IGLF+AQLSS AYQLEGMA+LG
Sbjct: 267 AVSVDQSRWENGFHAEAAEMIAGKWLKIWIEIGAVLSSIGLFEAQLSSSAYQLEGMAELG 326

Query: 325 FLPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEFGSFIWL 384
           FLPK FG RS  FNTPWVGILIS ++ +G+S M F D+ISSANFLY+LGM LEF SFIWL
Sbjct: 327 FLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFTDIISSANFLYTLGMFLEFASFIWL 386

Query: 385 RKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFGMGCYFLI 444
           R+K   LKRP+RVPL + GLVVMCLIPS FL+ I+  A K V+LI G+MT+  +G YFLI
Sbjct: 387 RRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILVFATKIVYLICGVMTIGAIGWYFLI 446

Query: 445 KFFKPEMGFELH 457
            +F+    FE +
Sbjct: 447 NYFRKTKIFEFN 456

BLAST of Tan0014566 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 434.5 bits (1116), Expect = 1.1e-121
Identity = 214/413 (51.82%), Postives = 292/413 (70.70%), Query Frame = 0

Query: 26  KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALITAELATT 85
           KK+++LPL+FLI++EV+GGPFG E +VKAAGP LAI GF +FPFIWSIPEALITAE+ T 
Sbjct: 51  KKITVLPLVFLIFYEVSGGPFGIEDSVKAAGPLLAIVGFIVFPFIWSIPEALITAEMGTM 110

Query: 86  FPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPILSSGFPRF 145
           FP NGG+V+W   A GP+WGF  G  K+ SGVI+ A YPIL +DYLK  +PIL SG PR 
Sbjct: 111 FPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRV 170

Query: 146 AAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWVVSGEKGT 205
           AA L  T AL++LNY GL++VG  A+ LG+ S+ PF++MS  +IP L   RW+V  +K  
Sbjct: 171 AAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMK 230

Query: 206 EKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIPLMAVIGA 265
             NW L+ NTLFWNLN+WD+ STL GEVE P K+ P+ALF A +L   +Y+ P++   GA
Sbjct: 231 GVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGA 290

Query: 266 LPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLEGMADLGF 325
           + LDQK W  GYFAD G+++ G WL +W++  A  S +G+F A++SS ++QL GMA+ G 
Sbjct: 291 IALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGM 350

Query: 326 LPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEFGSFIWLR 385
           LP++F +RS ++ TPWVGIL S   V+ +S + F +++++ N LY  GMVLEF +F+ LR
Sbjct: 351 LPEVFAKRS-RYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLR 410

Query: 386 KKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFGM 439
            K     RPF++P+GV G V+MC+ P+  +  IMA  N  V L+S    + G+
Sbjct: 411 MKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGL 462

BLAST of Tan0014566 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 428.7 bits (1101), Expect = 6.0e-120
Identity = 228/451 (50.55%), Postives = 310/451 (68.74%), Query Frame = 0

Query: 3   GEIETIHQNLLHHVHDQPLN--QPSKKLSLLPLIFLIYFEVAGGPFGEELAVKA-AGPFL 62
           GE ETI       V+D+  +  +PS KL+LLPL+FLI++EV+GGPFG E +VK+  GP L
Sbjct: 2   GEEETI-------VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLL 61

Query: 63  AIAGFFIFPFIWSIPEALITAELATTFPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVIN 122
           A+ GF IFP IWSIPEAL+TAELAT+FP NGG+V+W   AFGPFWGF  G WK+FSGV++
Sbjct: 62  ALLGFLIFPLIWSIPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMD 121

Query: 123 LASYPILCIDYLKLLLPILSSGFPRFAAFLASTSALSFLNYTGLTVVGFTAIALGIVSLF 182
            A YP+L +DYLK   P+L     R  A L  T +L++LNY GL +VGF+A+ L + SL 
Sbjct: 122 NALYPVLFLDYLKHSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLC 181

Query: 183 PFLIMSLFAIPNLDFRRWVVSGEKGTEK-NWGLFFNTLFWNLNFWDNASTLAGEVEEPQK 242
           PF++M+L A+PN+  +RW+      T+K NW  +FNT+FWNLN+WD ASTLAGEV+ P K
Sbjct: 182 PFVVMALLAVPNIRPKRWLF---VDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGK 241

Query: 243 SYPKALFSAGVLACLAYLIPLMAVIGALPLDQK-NWESGYFADAGEILVGKWLKYWVEIG 302
           ++PKALF A +L   +YLIPLMA  GAL       W  GYFA+ G ++ G WLK W++  
Sbjct: 242 TFPKALFGAVLLVMGSYLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAA 301

Query: 303 AVLSMIGLFQAQLSSCAYQLEGMADLGFLPKLFGERSVKFNTPWVGILISTVVVVGVSSM 362
           A +S +GLF+A++SS A+QL GM+++G LP  F +RS K+ TP + IL S   V+ +S M
Sbjct: 302 AAMSNLGLFEAEMSSDAFQLLGMSEIGMLPAFFAQRS-KYGTPTISILCSATGVIFLSWM 361

Query: 363 EFVDLISSANFLYSLGMVLEFGSFIWLRKKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLY 422
            F ++I   NFLY+LGM+LEF +F+ LR KK  L RP+RVPL   G+ ++CL PS  ++ 
Sbjct: 362 SFQEIIEFLNFLYALGMLLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVIL 421

Query: 423 IMALANKAVWLISGLMTLFGMGCYFLIKFFK 449
           +M LA    +LISG++ + G   Y  +   K
Sbjct: 422 VMVLAAPKTFLISGVIIVLGFCLYPFLTLVK 441

BLAST of Tan0014566 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 417.5 bits (1072), Expect = 1.4e-116
Identity = 202/412 (49.03%), Postives = 290/412 (70.39%), Query Frame = 0

Query: 26  KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALITAELATT 85
           +K+S+LPL+FLI++EV+GGPFG E +V AAGP LA+ GF IFPFIWSIPEALITAE+ T 
Sbjct: 47  RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLGFVIFPFIWSIPEALITAEMGTM 106

Query: 86  FPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPILSSGFPRF 145
           +P NGG+V+W   A GPFWGF  G  K+ SGVI+ A YP+L +DYLK  +P L SG PR 
Sbjct: 107 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 166

Query: 146 AAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWVVSGEKGT 205
           A+ L  T  L++LNY GLT+VG+ A+ +G+ S+ PF +M L +IP L+  RW+V      
Sbjct: 167 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 226

Query: 206 EKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIPLMAVIGA 265
             NW L+ NTLFWNLN+WD+ STLAGEVE P  + PKALF   +L   +Y+ PL+A IGA
Sbjct: 227 --NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGA 286

Query: 266 LPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLEGMADLGF 325
           +PL+++ W  GYF+D  + L G WL++WV+  A  S +G+F A++SS ++QL GMA+ G 
Sbjct: 287 IPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGM 346

Query: 326 LPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEFGSFIWLR 385
           LP+ F +RS ++ TP +GIL S   VV +S + F +++++ N LY +GM+LEF +F+ +R
Sbjct: 347 LPEFFAKRS-RYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMR 406

Query: 386 KKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFG 438
            K     RP+++P+G TG ++MC+ P+  +  ++AL++  V  +S +M + G
Sbjct: 407 MKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIG 455

BLAST of Tan0014566 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 417.5 bits (1072), Expect = 1.4e-116
Identity = 202/412 (49.03%), Postives = 290/412 (70.39%), Query Frame = 0

Query: 26  KKLSLLPLIFLIYFEVAGGPFGEELAVKAAGPFLAIAGFFIFPFIWSIPEALITAELATT 85
           +K+S+LPL+FLI++EV+GGPFG E +V AAGP LA+ GF IFPFIWSIPEALITAE+ T 
Sbjct: 31  RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLGFVIFPFIWSIPEALITAEMGTM 90

Query: 86  FPGNGGFVIWAYEAFGPFWGFLMGTWKFFSGVINLASYPILCIDYLKLLLPILSSGFPRF 145
           +P NGG+V+W   A GPFWGF  G  K+ SGVI+ A YP+L +DYLK  +P L SG PR 
Sbjct: 91  YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 150

Query: 146 AAFLASTSALSFLNYTGLTVVGFTAIALGIVSLFPFLIMSLFAIPNLDFRRWVVSGEKGT 205
           A+ L  T  L++LNY GLT+VG+ A+ +G+ S+ PF +M L +IP L+  RW+V      
Sbjct: 151 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 210

Query: 206 EKNWGLFFNTLFWNLNFWDNASTLAGEVEEPQKSYPKALFSAGVLACLAYLIPLMAVIGA 265
             NW L+ NTLFWNLN+WD+ STLAGEVE P  + PKALF   +L   +Y+ PL+A IGA
Sbjct: 211 --NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGA 270

Query: 266 LPLDQKNWESGYFADAGEILVGKWLKYWVEIGAVLSMIGLFQAQLSSCAYQLEGMADLGF 325
           +PL+++ W  GYF+D  + L G WL++WV+  A  S +G+F A++SS ++QL GMA+ G 
Sbjct: 271 IPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGM 330

Query: 326 LPKLFGERSVKFNTPWVGILISTVVVVGVSSMEFVDLISSANFLYSLGMVLEFGSFIWLR 385
           LP+ F +RS ++ TP +GIL S   VV +S + F +++++ N LY +GM+LEF +F+ +R
Sbjct: 331 LPEFFAKRS-RYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMR 390

Query: 386 KKKEGLKRPFRVPLGVTGLVVMCLIPSGFLLYIMALANKAVWLISGLMTLFG 438
            K     RP+++P+G TG ++MC+ P+  +  ++AL++  V  +S +M + G
Sbjct: 391 MKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIG 439

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHN77.3e-16365.97Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9FFL11.5e-12051.82Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
Q9LH398.4e-11950.55Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9C6S51.9e-11549.03Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
A2X8M82.0e-11249.30Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Match NameE-valueIdentityDescription
RXH85337.11.5e-18168.70hypothetical protein DVH24_042105 [Malus domestica][more]
TQE02057.11.5e-18168.70hypothetical protein C1H46_012376 [Malus baccata][more]
KAF3949085.12.0e-18170.68hypothetical protein CMV_024993 [Castanea mollissima][more]
XP_042957702.12.6e-18168.68probable polyamine transporter At3g13620 [Carya illinoinensis] >KAG6630127.1 hyp... [more]
PQP93813.17.4e-18167.02putative polyamine transporter [Prunus yedoensis var. nudiflora][more]
Match NameE-valueIdentityDescription
A0A498IT947.2e-18268.70Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_042105 PE=3 SV=1[more]
A0A540MTD07.2e-18268.70Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_012376 PE=3 SV=1[more]
A0A314XR843.6e-18167.02Putative polyamine transporter OS=Prunus yedoensis var. nudiflora OX=2094558 GN=... [more]
A0A2I4EDQ53.6e-18168.46probable polyamine transporter At3g13620 OS=Juglans regia OX=51240 GN=LOC1089886... [more]
A0A5E4F7W11.0e-18067.31PREDICTED: probable polyamine transporter OS=Prunus dulcis OX=3755 GN=ALMOND_2B0... [more]
Match NameE-valueIdentityDescription
AT3G13620.15.2e-16465.97Amino acid permease family protein [more]
AT5G05630.11.1e-12151.82Amino acid permease family protein [more]
AT3G19553.16.0e-12050.55Amino acid permease family protein [more]
AT1G31830.11.4e-11649.03Amino acid permease family protein [more]
AT1G31830.21.4e-11649.03Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 34..419
e-value: 5.7E-34
score: 117.7
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 24..453
e-value: 1.3E-55
score: 190.8
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 17..466
e-value: 1.2E-61
score: 206.2
NoneNo IPR availablePANTHERPTHR45826:SF8BNAC05G39680D PROTEINcoord: 22..458
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 22..458

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0014566.1Tan0014566.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity