Tan0014479 (gene) Snake gourd v1

Overview
NameTan0014479
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionMembrane protein of ER body-like protein
LocationLG06: 14012944 .. 14017059 (-)
RNA-Seq ExpressionTan0014479
SyntenyTan0014479
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGACAATGGAATCCACCACCCCCGAATGGGGACACACCACAGGCTCACGGCATGTGCTCAATCTCTTCGGGGACACACCCCAGGCTCATAGTCTATACACAAACTCTGCGGGGACACACCCTAGGTTCACCTCACCGCTAGCATAGAGTCGTGTCATCAACACACTCTATGGAGATCAACCCCTAAGCTAGCATGGGACAATGGAATCCACCACTCTCAAATGGGGACACACCTCAGGTTCACAACTTATGCTCAATCTCTACGGGGACATACCCCAAGCTCACCTCACCGTTAGCATAGAGTCGTGTTATCAACATGCTCTATGGAGATCAAGCCCCTAAACTAGCATGGGACAATGGAATCCACCACTTCCCGAATGGGGACACACCTCAGGTTCACAACCTATACTCAATCTCGTCAATCTAGAGGTTCCGACTCGCTCCTCTCTCAAGTGTAACTCAGGGTGTAGTAAGTACTGGGTTCCAGGTTTTATAAGGTTACTAGGGGTTCTAACTAGACATTCCTAATATCCTCGACCCATTCTCAAGGGTCCCAAATCCAGCTAGGCTCTCACTGTAACCAAGCTTTAACCCAAATCAATCACCTATTAGGAATGCTAGGTACAACCTGAACAATCAACCCCTACCTCACATAGTAAGACACATAGTCCATAGAAACATGATAAATACAATCAAACATGATTCAAAGACTTAGGATAACATGCACATAATCATGCCAAGTCCAAAAGCTCATAAAAACATGCTACACAGGTGCCATAAACACTTAGATAGCTAAACCAAAGTCTAATGCTAGAAACAACTCAAGAATACACAAACAAGCAATGTCAATCGCCTTACTCCTTCACTTAGGAGTTTGGTTTTAATCTAAAGACTACTTACCTTTGCAGAAAAAAGATTAACTCAAGAATATGTAAACAAAGAAATGTTGAACGCTTTGCTCCTTCACCTAGGAGCTTGGCTTTAAATCCAAAAGTTACTTACCTTATGCTTGAAGATTCAGGAAAAGAAATTGTGCCCCTTAAACAACAATAATGGGGTCTAAAACAAACTTAAGAAGTTGAAGCTGATTGGGCTAGATCAACTCCAGCGAGCGACGGCGGTCCGACGACTTTCGGTGGTGGTCCGACGACTTTCCGGCGGTGGTCCACGTAAAACTCCTGTGGCTTTTCGGCGACTGTCCGACGAATTTCCGGCGACTCTTTGGCGGTGGTCCGGCGAAACTCCGCGCGGCGGTCCAACGACTTTCCGACGGTGGTCCGGCGGGATGAAGAATGTGAAGAAAAGAAGAAGAAAAAGAAGAAAGAGAAAAAGAGAAGAGTTTTGAAGTGCACCCTTTAAGTGGCAAATACTCTAGTTTAAAAAGGTACCAAAAAGTCTTAATCTTTTTAGAACAAAGAATTCTAATACTTTTTTAGAGAAAGGAAACCAAAACCTTTTTAAAACAAAAATCCTAATCTTTTTTATAAACTTTGAAACACCAATTACAACACCTAATTTTATGGGTGTCACATCAAATTTACCTTTTATAAAAATTTTAAGAAGCCTTTTTATCGACGAAAAACCTTTTTTATCGTCGAGTCAGATTCAAGGGAGGTCATTCATCTTCTTAATCTCTCTAAGTTTGATATGTTTAAAGTGTGTCAGGATGATATTACTTCCTTGTCTAGTAGTTTTTGCTCCATTATTTTTGTAACATCCCTAGAGAGAAGAGCAAAGTTGTCCACAACCTCGCAACTTTGGTATCTTTTTCAGGGAGCTCTTTGGTCTGGAAAGATGATTTTCCAACGGACTTTTCCTCTCCTTAGTTTAGGAGGAGGGACTTTTGGGGTAGCTCGTTCTTTTATGGCATAAAAAAAAAATTGTATATCACAAAACAAAGAATTGTTTAGATATATAACCAGCTTTAATTTTTTTATAAAAAAAAAAGTGGTAAAAATAAAATTGTTTTCAAATGAAGTGTGCTACCTTCTTTTGCAGGTTTCAGTGAAGGAAGTAGTAGTAGTGAGAACACTAGAGGTGAAACAAGCACAACACATTCAATTAGAGATGGTATCCCTGAAAATGGAGTATCAGTAGCAAGGCCATGCATTAATAATATTGGAACACAGCACATTGTTCCTTTAGAGTCAGCCAATGGATTGTCAGTAGTTGGCGAACAATCAAGTGGAAATTGTAGTGTCATTACAAATCAAAATCTAGCTACTGAAGATCAGGCAACCACAAATACAGATACTGAGACAATTCAAACTCAAACTCTAGTTCGTCGAGAAGAAATGGTTTTAGTTAGTGCAATTCCACAACCAAGTGGAAGTATTATAAAGATAGAAATTGCGAGAAGCATAGTCTATGGTGGTTTAACTGAATTGATTGCAAGCTTAGGCATTGTAACATCTGCAGCAGCTACCACAACTACCACTCGTAAAATTAATATTATTTTTCAAACTATTTCATTTTGCCTTTCCCCCTTTCTTTTATATACGCATGCTTTCGTATTTCTACACTAATTGTGTTTCATAAATATGCAGGGAAAATTGTAGCTGTTTCAATAGCAAGCCTGATTACGGGATTGTTCATCATGACTTATAAAGTAAGTAGTAAAATCCCCCTTAGACTAATATGATATTGCCTATATATTGGATTAAGTATGCACTAATATATATAACTAGTCCACATTTAGAAATTTATGAATATAGCATACCAATTAGCTGACCACCTTTCTTACTGCAATTATTTAACTTTAATTTCAAAAGATGAAAGTTATCTTTTTAATTGTGAACTCACCTATTAAACTAGTATTGATATGATTAAATTTACCATAATCTATTAGTTTAGACCCCGTTTGATAACAATTTGATTTTTTGTTTTTTAGTTTTTGAAAATTGCACCTAAAAATAATTCTTTTGCTCATGAGTAGTATCTATGTTTTCTTATCTACATTATATCTATGTTTTCAAAAATCAAGCTAAGTCTTGAAAAAAAAATGGTTTTCAAAAACTTGTTTTTGTTTTTGACATTTGACTATAAACTTAAATGATTACTCAAGAAAGATGGAAATTATTGTAGAGAAGATGAAGAGAAATTGTAAAAAAATAAACATAATTTTCAAAACCAAAAACCAAAAACCAAATTGTTATCAAACAGAACCTTAAGTTTTTGGGTTGATTGGTGGTTTAAACATGGTATCAGAGCAGGAAATCCTGTGTTTAAACTCTACTCATGTCCATATAATTAAATTCACCATAACCTTAAGCTTTTGAGGTCGTTCAAAAACAAGTAATAATATCAATTCCTTCTCCAATTAATATTGATTTCCACTTGTTGGGTATTTTCTCTAAATTTTTCAAGCCAACAAGTGAGGGGTTGTGTTAGACTAGTTGATATAATTAAATTTACGGTGAGCTATATGTTAAGTTTTTAGATTATATTGATTGGTGATTTAACCTCACTCATTGTTAAAGGTGAAAATTTTAGAGAGAGAAAATAAATTTAAAATGATTTGCTGGAATATTTAATCTCAAACAAACTTGTATTTGAAAAACTGAATGGATCCCAAAAGTATTTTCCTTCCTTTTTCTCTCGTTCCCAATGTACTGGGAAATTACAGATATCAGAACTTTGGATGAATAATGACAAAAGTAGAGAAGATGAAGCAGAAGCTAAAGAAGAAGAAAGGTACGAAAATATATTTGGAGATAAAAAAAATTATCGGCTCCATTTCACAGTGGCTATTCTATCATTCGTATGTTTTGGATTGGTACCTCCTCTTGTTTATGGCTTCACATTTAGGAAGCCTGAGGACAAGTTACTCAAGATCTCAACAGTTACAATCGTCGCTGTTTTTTGCACTGGATTACTTGGTGTTGGCAAAGCTTACATTAAGAGACCATCTAATTGTGTTGAATATATCAATATCATTGGTAAATGCATTGTTGATGCAATTGGGACGTCGGTGCTTACAACTCTTGGAGGTGACTTAATTGAAAATTATATTCAGCAAATTGGTTGGTTTGAGCCAAATAAAATACCAACCTTGTTCCAGCCAGGCTTGAGTTTGAGTTTTGAGAATGGTACTTGGGGTTGGGGAGCCTTTTGA

mRNA sequence

ATGGGACAATGGAATCCACCACCCCCGAATGGGGACACACCACAGGCTCACGGCATGTGCTCAATCTCTTCGGGGACACACCCCAGCATGGGACAATGGAATCCACCACTTCCCGAATGGGGACACACCTCAGGTTCACAACCTATACTCAATCTCGTCAATCTAGAGGTTCCGACTCGCTCCTCTCTCAAGTGTTTCAGTGAAGGAAGTAGTAGTAGTGAGAACACTAGAGGTGAAACAAGCACAACACATTCAATTAGAGATGGTATCCCTGAAAATGGAGTATCAGTAGCAAGGCCATGCATTAATAATATTGGAACACAGCACATTGTTCCTTTAGAGTCAGCCAATGGATTGTCAGTAGTTGGCGAACAATCAAGTGGAAATTGTAGTGTCATTACAAATCAAAATCTAGCTACTGAAGATCAGGCAACCACAAATACAGATACTGAGACAATTCAAACTCAAACTCTAGTTCGTCGAGAAGAAATGGTTTTAGTTAGTGCAATTCCACAACCAAGTGGAAGTATTATAAAGATAGAAATTGCGAGAAGCATAGTCTATGGTGGTTTAACTGAATTGATTGCAAGCTTAGGCATTGTAACATCTGCAGCAGCTACCACAACTACCACTCGGAAAATTGTAGCTGTTTCAATAGCAAGCCTGATTACGGGATTGTTCATCATGACTTATAAAATATCAGAACTTTGGATGAATAATGACAAAAGTAGAGAAGATGAAGCAGAAGCTAAAGAAGAAGAAAGGTACGAAAATATATTTGGAGATAAAAAAAATTATCGGCTCCATTTCACAGTGGCTATTCTATCATTCGTATGTTTTGGATTGGTACCTCCTCTTGTTTATGGCTTCACATTTAGGAAGCCTGAGGACAAGTTACTCAAGATCTCAACAGTTACAATCGTCGCTGTTTTTTGCACTGGATTACTTGGTGTTGGCAAAGCTTACATTAAGAGACCATCTAATTGTGTTGAATATATCAATATCATTGGTAAATGCATTGTTGATGCAATTGGGACGTCGGTGCTTACAACTCTTGGAGGTGACTTAATTGAAAATTATATTCAGCAAATTGGTTGGTTTGAGCCAAATAAAATACCAACCTTGTTCCAGCCAGGCTTGAGTTTGAGTTTTGAGAATGGTACTTGGGGTTGGGGAGCCTTTTGA

Coding sequence (CDS)

ATGGGACAATGGAATCCACCACCCCCGAATGGGGACACACCACAGGCTCACGGCATGTGCTCAATCTCTTCGGGGACACACCCCAGCATGGGACAATGGAATCCACCACTTCCCGAATGGGGACACACCTCAGGTTCACAACCTATACTCAATCTCGTCAATCTAGAGGTTCCGACTCGCTCCTCTCTCAAGTGTTTCAGTGAAGGAAGTAGTAGTAGTGAGAACACTAGAGGTGAAACAAGCACAACACATTCAATTAGAGATGGTATCCCTGAAAATGGAGTATCAGTAGCAAGGCCATGCATTAATAATATTGGAACACAGCACATTGTTCCTTTAGAGTCAGCCAATGGATTGTCAGTAGTTGGCGAACAATCAAGTGGAAATTGTAGTGTCATTACAAATCAAAATCTAGCTACTGAAGATCAGGCAACCACAAATACAGATACTGAGACAATTCAAACTCAAACTCTAGTTCGTCGAGAAGAAATGGTTTTAGTTAGTGCAATTCCACAACCAAGTGGAAGTATTATAAAGATAGAAATTGCGAGAAGCATAGTCTATGGTGGTTTAACTGAATTGATTGCAAGCTTAGGCATTGTAACATCTGCAGCAGCTACCACAACTACCACTCGGAAAATTGTAGCTGTTTCAATAGCAAGCCTGATTACGGGATTGTTCATCATGACTTATAAAATATCAGAACTTTGGATGAATAATGACAAAAGTAGAGAAGATGAAGCAGAAGCTAAAGAAGAAGAAAGGTACGAAAATATATTTGGAGATAAAAAAAATTATCGGCTCCATTTCACAGTGGCTATTCTATCATTCGTATGTTTTGGATTGGTACCTCCTCTTGTTTATGGCTTCACATTTAGGAAGCCTGAGGACAAGTTACTCAAGATCTCAACAGTTACAATCGTCGCTGTTTTTTGCACTGGATTACTTGGTGTTGGCAAAGCTTACATTAAGAGACCATCTAATTGTGTTGAATATATCAATATCATTGGTAAATGCATTGTTGATGCAATTGGGACGTCGGTGCTTACAACTCTTGGAGGTGACTTAATTGAAAATTATATTCAGCAAATTGGTTGGTTTGAGCCAAATAAAATACCAACCTTGTTCCAGCCAGGCTTGAGTTTGAGTTTTGAGAATGGTACTTGGGGTTGGGGAGCCTTTTGA

Protein sequence

MGQWNPPPPNGDTPQAHGMCSISSGTHPSMGQWNPPLPEWGHTSGSQPILNLVNLEVPTRSSLKCFSEGSSSSENTRGETSTTHSIRDGIPENGVSVARPCINNIGTQHIVPLESANGLSVVGEQSSGNCSVITNQNLATEDQATTNTDTETIQTQTLVRREEMVLVSAIPQPSGSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTYKISELWMNNDKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFTFRKPEDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLTTLGGDLIENYIQQIGWFEPNKIPTLFQPGLSLSFENGTWGWGAF
Homology
BLAST of Tan0014479 vs. ExPASy Swiss-Prot
Match: Q8W4P8 (Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.9e-27
Identity = 78/210 (37.14%), Postives = 123/210 (58.57%), Query Frame = 0

Query: 168 SAIPQPSGSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLF 227
           S  P+  G+  K+EI +SIVYGGLTE I SL  VTSAAA+  +T  ++A+ +A+L +GL 
Sbjct: 401 SVEPRKGGN--KVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLL 460

Query: 228 IMTYKISELWMNNDKSRED-------EAEAKEEERYENIFGDKKNYRLHFTVAILSFVCF 287
           +  + + EL   N+K R+        E    EE+RYE + G ++  R+H  +AI SFV F
Sbjct: 461 LTVHSLQEL--INEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIF 520

Query: 288 GLVPPLVYGFTFRKPEDKL--LKISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGK 347
           GL+PPLVYGF+FRK  +K    K+  V  V++ C  LL + KAY+ +     +Y+  + +
Sbjct: 521 GLIPPLVYGFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKR---DYVKTLFR 580

Query: 348 CIVDAIGTSVLTTLGGDLIENYIQQIGWFE 369
               A   S  +   G L+  ++++ G+++
Sbjct: 581 YTTTATTASGFSQFVGYLVSQWLEKSGFYD 603

BLAST of Tan0014479 vs. ExPASy Swiss-Prot
Match: F4KFS7 (Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.7e-25
Identity = 66/197 (33.50%), Postives = 117/197 (59.39%), Query Frame = 0

Query: 172 QPSGSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTY 231
           QP G    IE+ +S VYGGLTE I SLG+V+SA+A+ ++T  I+A+++A+L  GL ++  
Sbjct: 336 QPDGERFAIELLKSTVYGGLTETITSLGVVSSASASGSSTMNILALAVANLAGGLIVLAQ 395

Query: 232 KISELWMNNDKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFT 291
              +L  ++D         +E++RYE + G +   R+H  VA++S++ FGL+PPLVY F+
Sbjct: 396 NFQDLRNSSD---------QEKDRYEELLGRRTKSRIHILVAVMSYIFFGLIPPLVYAFS 455

Query: 292 FRKPEDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCV----EYINIIGKCIVDAIGTS 351
           F +   K  K+ +V + ++ C  LLG  K Y+++P+N       Y+          + + 
Sbjct: 456 FYETGIKNYKLISVFLGSLVCVILLGSIKVYVRKPTNSCGSTKAYLKSAAYYTSIVVASC 515

Query: 352 VLTTLGGDLIENYIQQI 365
            ++ + GD++  YI+++
Sbjct: 516 GISYVVGDIMGEYIEKL 523

BLAST of Tan0014479 vs. ExPASy Swiss-Prot
Match: Q8LPT3 (Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 6.6e-24
Identity = 80/258 (31.01%), Postives = 139/258 (53.88%), Query Frame = 0

Query: 121 VVGEQSSGNCSVITNQNLATEDQATTNTDTETIQTQTLVRREEMVLVSAIPQPSGSIIKI 180
           V G   +G+  VIT       D +  N   E    + L+RR   V+V           K+
Sbjct: 495 VTGVMDTGDRGVITGPANPEIDISAGNLLEEGSLREPLMRR---VVVQG--------RKL 554

Query: 181 EIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTYKISEL---- 240
           EI +SIVYGGL E I SLG+++SAA +  +   I+ + +A+L+ GL ++ + + EL    
Sbjct: 555 EILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGGLILIIHNLQELREEE 614

Query: 241 -WMNNDKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFTFRKP 300
                 +  +     +EE RY+ + G ++N+ LH TVAILSF+  G++PP+VY F+F + 
Sbjct: 615 PIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFSFSEK 674

Query: 301 EDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLTTLGGD 360
            +K  K+++V   ++FC  LL + KA+++ P     Y+  I      A+  S ++ + G+
Sbjct: 675 HNKDYKVASVFGASLFCIVLLAIAKAHVRYPRG--SYLKSILYYGSIAVSVSGISYVVGN 734

Query: 361 LIENYIQQIGWFEPNKIP 374
            +E  +++ GW + ++ P
Sbjct: 735 FLEQLLEKHGWSDGSETP 739

BLAST of Tan0014479 vs. NCBI nr
Match: XP_038897302.1 (membrane protein of ER body 1-like isoform X3 [Benincasa hispida])

HSP 1 Score: 339.7 bits (870), Expect = 3.3e-89
Identity = 199/341 (58.36%), Postives = 242/341 (70.97%), Query Frame = 0

Query: 61  SSLKCFSEGSSSSENTRGETSTTHSIRDGIPENGVSVARPCINNIGTQHIVPLESANGLS 120
           S+L  F +G SSSEN+RGETS T           + V+ P +                + 
Sbjct: 75  SNLVSFHQG-SSSENSRGETSCT--------TKSIGVSDPTLQ---------------IY 134

Query: 121 VVGEQSSGN---CSVITNQNLATEDQATTNTDTETIQTQTLVRREEMVLVSA--IPQP-S 180
            VGEQS+ +    SV  N   A   +    T+ ETI    +V ++E++L+ A  I +P S
Sbjct: 135 EVGEQSNDDNEEYSVDNNITAAAASEPHVITEIETIPNNVVVIQDEVLLIEATIIQEPIS 194

Query: 181 GSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTYKIS 240
           GS +KIEI++SIVYGGLTELIASLGIVTSAA T  TT KI+AVS+ASLITG F+M YKIS
Sbjct: 195 GSTLKIEISKSIVYGGLTELIASLGIVTSAATTNATTGKILAVSLASLITGFFVMAYKIS 254

Query: 241 ELWMNNDKSREDEAEAKE-EERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFTFR 300
           ELW+ N +S  DEAE +E  ERYEN+FGD+KNYRLHF VA+LSFV FGL+PPLVY FTF 
Sbjct: 255 ELWIKNHESGTDEAEDQENSERYENVFGDRKNYRLHFGVALLSFVLFGLMPPLVYCFTFC 314

Query: 301 KPEDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLTTLG 360
           KPEDK+LK+ TVTIV V C  LLGV KAYI+R +NCVE++NIIG C+VDA+GTSVLTT+G
Sbjct: 315 KPEDKILKVPTVTIVVVLCIALLGVAKAYIERKTNCVEFVNIIGNCVVDALGTSVLTTIG 374

Query: 361 GDLIENYIQQIGWFEPNKIPTLFQPGLSLSFENGTWGWGAF 395
           G+LIENYI + GWF+PNKIPTLFQPG   SFENGT  WG F
Sbjct: 375 GELIENYIDKAGWFDPNKIPTLFQPG--FSFENGTSAWGGF 389

BLAST of Tan0014479 vs. NCBI nr
Match: XP_038897303.1 (uncharacterized protein LOC120085412 isoform X4 [Benincasa hispida])

HSP 1 Score: 331.6 bits (849), Expect = 9.0e-87
Identity = 197/341 (57.77%), Postives = 239/341 (70.09%), Query Frame = 0

Query: 61  SSLKCFSEGSSSSENTRGETSTTHSIRDGIPENGVSVARPCINNIGTQHIVPLESANGLS 120
           S+L  F +G SSSEN+RGETS T           + V+ P +                + 
Sbjct: 75  SNLVSFHQG-SSSENSRGETSCT--------TKSIGVSDPTLQ---------------IY 134

Query: 121 VVGEQSSGN---CSVITNQNLATEDQATTNTDTETIQTQTLVRREEMVLVSA--IPQP-S 180
            VGEQS+ +    SV  N   A   +    T+ ETI    +V ++E++L+ A  I +P S
Sbjct: 135 EVGEQSNDDNEEYSVDNNITAAAASEPHVITEIETIPNNVVVIQDEVLLIEATIIQEPIS 194

Query: 181 GSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTYKIS 240
           GS +KIEI++SIVYGGLTELIASLGIVTSAA T  TT    AVS+ASLITG F+M YKIS
Sbjct: 195 GSTLKIEISKSIVYGGLTELIASLGIVTSAATTNATT----AVSLASLITGFFVMAYKIS 254

Query: 241 ELWMNNDKSREDEAEAKE-EERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFTFR 300
           ELW+ N +S  DEAE +E  ERYEN+FGD+KNYRLHF VA+LSFV FGL+PPLVY FTF 
Sbjct: 255 ELWIKNHESGTDEAEDQENSERYENVFGDRKNYRLHFGVALLSFVLFGLMPPLVYCFTFC 314

Query: 301 KPEDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLTTLG 360
           KPEDK+LK+ TVTIV V C  LLGV KAYI+R +NCVE++NIIG C+VDA+GTSVLTT+G
Sbjct: 315 KPEDKILKVPTVTIVVVLCIALLGVAKAYIERKTNCVEFVNIIGNCVVDALGTSVLTTIG 374

Query: 361 GDLIENYIQQIGWFEPNKIPTLFQPGLSLSFENGTWGWGAF 395
           G+LIENYI + GWF+PNKIPTLFQPG   SFENGT  WG F
Sbjct: 375 GELIENYIDKAGWFDPNKIPTLFQPG--FSFENGTSAWGGF 385

BLAST of Tan0014479 vs. NCBI nr
Match: XP_038897300.1 (membrane protein of ER body-like protein isoform X1 [Benincasa hispida])

HSP 1 Score: 326.6 bits (836), Expect = 2.9e-85
Identity = 199/364 (54.67%), Postives = 242/364 (66.48%), Query Frame = 0

Query: 61  SSLKCFSEGSSSSENTRGETSTTHSIRDGIPENGVSVARPCINNIGTQHIVPLESANGLS 120
           S+L  F +G SSSEN+RGETS T           + V+ P +                + 
Sbjct: 75  SNLVSFHQG-SSSENSRGETSCT--------TKSIGVSDPTLQ---------------IY 134

Query: 121 VVGEQSSGN---CSVITNQNLATEDQATTNTDTETIQTQTLVRREEMVLVSA--IPQP-S 180
            VGEQS+ +    SV  N   A   +    T+ ETI    +V ++E++L+ A  I +P S
Sbjct: 135 EVGEQSNDDNEEYSVDNNITAAAASEPHVITEIETIPNNVVVIQDEVLLIEATIIQEPIS 194

Query: 181 GSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTT----------------------- 240
           GS +KIEI++SIVYGGLTELIASLGIVTSAA T  TT                       
Sbjct: 195 GSTLKIEISKSIVYGGLTELIASLGIVTSAATTNATTGKFFLIIPFLSILSSLYTCFHNA 254

Query: 241 RKIVAVSIASLITGLFIMTYKISELWMNNDKSREDEAEAKE-EERYENIFGDKKNYRLHF 300
            KI+AVS+ASLITG F+M YKISELW+ N +S  DEAE +E  ERYEN+FGD+KNYRLHF
Sbjct: 255 GKILAVSLASLITGFFVMAYKISELWIKNHESGTDEAEDQENSERYENVFGDRKNYRLHF 314

Query: 301 TVAILSFVCFGLVPPLVYGFTFRKPEDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCV 360
            VA+LSFV FGL+PPLVY FTF KPEDK+LK+ TVTIV V C  LLGV KAYI+R +NCV
Sbjct: 315 GVALLSFVLFGLMPPLVYCFTFCKPEDKILKVPTVTIVVVLCIALLGVAKAYIERKTNCV 374

Query: 361 EYINIIGKCIVDAIGTSVLTTLGGDLIENYIQQIGWFEPNKIPTLFQPGLSLSFENGTWG 395
           E++NIIG C+VDA+GTSVLTT+GG+LIENYI + GWF+PNKIPTLFQPG   SFENGT  
Sbjct: 375 EFVNIIGNCVVDALGTSVLTTIGGELIENYIDKAGWFDPNKIPTLFQPG--FSFENGTSA 412

BLAST of Tan0014479 vs. NCBI nr
Match: XP_038897301.1 (membrane protein of ER body-like protein isoform X2 [Benincasa hispida])

HSP 1 Score: 326.6 bits (836), Expect = 2.9e-85
Identity = 199/364 (54.67%), Postives = 242/364 (66.48%), Query Frame = 0

Query: 61  SSLKCFSEGSSSSENTRGETSTTHSIRDGIPENGVSVARPCINNIGTQHIVPLESANGLS 120
           S+L  F +G SSSEN+RGETS T           + V+ P +                + 
Sbjct: 57  SNLVSFHQG-SSSENSRGETSCT--------TKSIGVSDPTLQ---------------IY 116

Query: 121 VVGEQSSGN---CSVITNQNLATEDQATTNTDTETIQTQTLVRREEMVLVSA--IPQP-S 180
            VGEQS+ +    SV  N   A   +    T+ ETI    +V ++E++L+ A  I +P S
Sbjct: 117 EVGEQSNDDNEEYSVDNNITAAAASEPHVITEIETIPNNVVVIQDEVLLIEATIIQEPIS 176

Query: 181 GSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTT----------------------- 240
           GS +KIEI++SIVYGGLTELIASLGIVTSAA T  TT                       
Sbjct: 177 GSTLKIEISKSIVYGGLTELIASLGIVTSAATTNATTGKFFLIIPFLSILSSLYTCFHNA 236

Query: 241 RKIVAVSIASLITGLFIMTYKISELWMNNDKSREDEAEAKE-EERYENIFGDKKNYRLHF 300
            KI+AVS+ASLITG F+M YKISELW+ N +S  DEAE +E  ERYEN+FGD+KNYRLHF
Sbjct: 237 GKILAVSLASLITGFFVMAYKISELWIKNHESGTDEAEDQENSERYENVFGDRKNYRLHF 296

Query: 301 TVAILSFVCFGLVPPLVYGFTFRKPEDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCV 360
            VA+LSFV FGL+PPLVY FTF KPEDK+LK+ TVTIV V C  LLGV KAYI+R +NCV
Sbjct: 297 GVALLSFVLFGLMPPLVYCFTFCKPEDKILKVPTVTIVVVLCIALLGVAKAYIERKTNCV 356

Query: 361 EYINIIGKCIVDAIGTSVLTTLGGDLIENYIQQIGWFEPNKIPTLFQPGLSLSFENGTWG 395
           E++NIIG C+VDA+GTSVLTT+GG+LIENYI + GWF+PNKIPTLFQPG   SFENGT  
Sbjct: 357 EFVNIIGNCVVDALGTSVLTTIGGELIENYIDKAGWFDPNKIPTLFQPG--FSFENGTSA 394

BLAST of Tan0014479 vs. NCBI nr
Match: XP_022144855.1 (membrane protein of ER body 2-like isoform X1 [Momordica charantia])

HSP 1 Score: 315.5 bits (807), Expect = 6.7e-82
Identity = 183/340 (53.82%), Postives = 231/340 (67.94%), Query Frame = 0

Query: 67  SEGSSSSENTRGETSTTHSIRDGIP------ENGVSVARPCINNIGTQHIVPLESANGLS 126
           +EG  +       +ST H +   +P       NG S++ P + N   Q     +S   + 
Sbjct: 2   AEGPIALAAPSSSSSTAHCVNGKVPPRKNGLTNGCSMSMPDLKNGFNQESSSKKSTQDIV 61

Query: 127 VVGEQSSGNCSVITNQNLATEDQATTNTDTETIQTQ-----TLVRREEMV-----LVSAI 186
              +       + TNQN   +    T T+ ET+Q +     T  + E +V     L++A+
Sbjct: 62  PKNKPK----EIGTNQNEDNKQDGGTITEIETVQNEVNSSPTAPQGEVLVTSATQLLAAV 121

Query: 187 PQPSGSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMT 246
            +PSG+IIKIEI +SI+YGGLTELIASLG+VTSAA+T TTT K+VA+S+ASLITGLF+MT
Sbjct: 122 -EPSGTIIKIEIVKSILYGGLTELIASLGVVTSAASTKTTTGKVVALSLASLITGLFVMT 181

Query: 247 YKISELWMNNDKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGF 306
           YKIS+LWMN  ++   E+  + +E+YE +FGD+KNYRLHFT A+LSFV FGLVPPLVYGF
Sbjct: 182 YKISDLWMNKQQTGSHESNDENDEQYEELFGDRKNYRLHFTTAMLSFVSFGLVPPLVYGF 241

Query: 307 TFRKPEDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLT 366
           TFR PEDK LKI TVTI AV C  LLG  KAYI R +NCV YIN IGKCIVDA GT++LT
Sbjct: 242 TFRNPEDKNLKIPTVTIAAVLCIALLGAIKAYIGRQNNCVGYINTIGKCIVDATGTAILT 301

Query: 367 TLGGDLIENYIQQIGWFEPNKIPTLFQPGLSLSFENGTWG 391
           T+GGD+I NYI Q+GWFEPNKIPTLF P  S+SF N T G
Sbjct: 302 TIGGDIIGNYIDQLGWFEPNKIPTLFLP--SMSFHNRTRG 334

BLAST of Tan0014479 vs. ExPASy TrEMBL
Match: A0A6J1CSU1 (membrane protein of ER body 2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014436 PE=3 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 3.2e-82
Identity = 183/340 (53.82%), Postives = 231/340 (67.94%), Query Frame = 0

Query: 67  SEGSSSSENTRGETSTTHSIRDGIP------ENGVSVARPCINNIGTQHIVPLESANGLS 126
           +EG  +       +ST H +   +P       NG S++ P + N   Q     +S   + 
Sbjct: 2   AEGPIALAAPSSSSSTAHCVNGKVPPRKNGLTNGCSMSMPDLKNGFNQESSSKKSTQDIV 61

Query: 127 VVGEQSSGNCSVITNQNLATEDQATTNTDTETIQTQ-----TLVRREEMV-----LVSAI 186
              +       + TNQN   +    T T+ ET+Q +     T  + E +V     L++A+
Sbjct: 62  PKNKPK----EIGTNQNEDNKQDGGTITEIETVQNEVNSSPTAPQGEVLVTSATQLLAAV 121

Query: 187 PQPSGSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMT 246
            +PSG+IIKIEI +SI+YGGLTELIASLG+VTSAA+T TTT K+VA+S+ASLITGLF+MT
Sbjct: 122 -EPSGTIIKIEIVKSILYGGLTELIASLGVVTSAASTKTTTGKVVALSLASLITGLFVMT 181

Query: 247 YKISELWMNNDKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGF 306
           YKIS+LWMN  ++   E+  + +E+YE +FGD+KNYRLHFT A+LSFV FGLVPPLVYGF
Sbjct: 182 YKISDLWMNKQQTGSHESNDENDEQYEELFGDRKNYRLHFTTAMLSFVSFGLVPPLVYGF 241

Query: 307 TFRKPEDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLT 366
           TFR PEDK LKI TVTI AV C  LLG  KAYI R +NCV YIN IGKCIVDA GT++LT
Sbjct: 242 TFRNPEDKNLKIPTVTIAAVLCIALLGAIKAYIGRQNNCVGYINTIGKCIVDATGTAILT 301

Query: 367 TLGGDLIENYIQQIGWFEPNKIPTLFQPGLSLSFENGTWG 391
           T+GGD+I NYI Q+GWFEPNKIPTLF P  S+SF N T G
Sbjct: 302 TIGGDIIGNYIDQLGWFEPNKIPTLFLP--SMSFHNRTRG 334

BLAST of Tan0014479 vs. ExPASy TrEMBL
Match: A0A6J1JX70 (membrane protein of ER body 1-like OS=Cucurbita maxima OX=3661 GN=LOC111488291 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.3e-59
Identity = 139/266 (52.26%), Postives = 177/266 (66.54%), Query Frame = 0

Query: 140 TEDQA-----TTNTDTETIQTQTLVRREEMVLVSAIP----QPSGSIIKIEIARSIVYGG 199
           TE+Q+     T N + E +  Q LV      L+   P    Q S + I+ EI +S++YGG
Sbjct: 118 TEEQSNEVIYTHNGEQEVLCQQILVASTPHELIVTEPEIEEQQSATSIRTEIVKSVLYGG 177

Query: 200 LTELIASLGIVTSAAATTTTTRKIVAVSI-------ASLITGLFIMTYKISELWMNNDKS 259
           LTELIASLGIVTS+A+T  TT K     +        S  T + +  + IS+ W +  +S
Sbjct: 178 LTELIASLGIVTSSASTGATTDKQSNKEVIDTHRKNGSSFT-IKLDYWTISKHWKSGQES 237

Query: 260 REDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFTFRKPEDKLLKIS 319
             DE   +E +RYE +FGD+KNYRLH TVAILSF+ FGLVPPL+YGFTFRKPEDK LKI 
Sbjct: 238 GPDEQREEEVDRYEQVFGDRKNYRLHLTVAILSFLIFGLVPPLLYGFTFRKPEDKFLKIP 297

Query: 320 TVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLTTLGGDLIENYIQQ 379
           TVTIV+V C GLLGVGKAYI++P NC+ Y++IIG CI DAIGTS +TTLGG+LI+NY+ +
Sbjct: 298 TVTIVSVLCIGLLGVGKAYIEKPENCLVYVHIIGGCIADAIGTSAVTTLGGELIQNYVDR 357

Query: 380 IGW--FEPNKIPTLFQPGLSLSFENG 388
            GW  FEPNK+PT       L   +G
Sbjct: 358 AGWFDFEPNKVPTFSYQAWILGIRHG 382

BLAST of Tan0014479 vs. ExPASy TrEMBL
Match: A0A5A7SK09 (Membrane protein of ER body-like protein isoform X4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G00870 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 2.6e-39
Identity = 95/210 (45.24%), Postives = 131/210 (62.38%), Query Frame = 0

Query: 181 EIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTYKISELWMNN 240
           EI +SIVYGGL E I SLGIVTSAA+  T T  IV +S+A+LI+GLFI+ + ++ L    
Sbjct: 628 EIVKSIVYGGLAESITSLGIVTSAASANTGTGNIVVLSLANLISGLFILGHNLTGLKSEQ 687

Query: 241 DKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFTFRKPEDKLL 300
            ++  +  +    +RYE + G+++NY LHF +AI SFV FGLVPPLVYGF+F K  DK L
Sbjct: 688 FRTSNETDDDDHVDRYEVVLGNRENYILHFVLAIFSFVLFGLVPPLVYGFSFTKSNDKDL 747

Query: 301 KISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLTTLGGDLIENY 360
           K++ V   ++ C  LL +GKAYI+RP+    YI  +   IV A G    + L G+LI+  
Sbjct: 748 KLTAVAGASLLCITLLALGKAYIQRPNRWDVYIKTVVSYIVIAAGAGGFSYLAGNLIDKC 807

Query: 361 IQQIGWFEPNKIPTLFQPGLSLSFENGTWG 391
           +++ GWFE N    L  P   +S     WG
Sbjct: 808 VKKYGWFEENPAFNLGLPLPEMSLVKAAWG 837

BLAST of Tan0014479 vs. ExPASy TrEMBL
Match: A0A5D3CME8 (Membrane protein of ER body-like protein isoform X4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold263G00400 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 2.6e-39
Identity = 95/210 (45.24%), Postives = 131/210 (62.38%), Query Frame = 0

Query: 181 EIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTYKISELWMNN 240
           EI +SIVYGGL E I SLGIVTSAA+  T T  IV +S+A+LI+GLFI+ + ++ L    
Sbjct: 588 EIVKSIVYGGLAESITSLGIVTSAASANTGTGNIVVLSLANLISGLFILGHNLTGLKSEQ 647

Query: 241 DKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFTFRKPEDKLL 300
            ++  +  +    +RYE + G+++NY LHF +AI SFV FGLVPPLVYGF+F K  DK L
Sbjct: 648 FRTSNETDDDDHVDRYEVVLGNRENYILHFVLAIFSFVLFGLVPPLVYGFSFTKSNDKDL 707

Query: 301 KISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLTTLGGDLIENY 360
           K++ V   ++ C  LL +GKAYI+RP+    YI  +   IV A G    + L G+LI+  
Sbjct: 708 KLTAVAGASLLCITLLALGKAYIQRPNRWDVYIKTVVSYIVIAAGAGGFSYLAGNLIDKC 767

Query: 361 IQQIGWFEPNKIPTLFQPGLSLSFENGTWG 391
           +++ GWFE N    L  P   +S     WG
Sbjct: 768 VKKYGWFEENPAFNLGLPLPEMSLVKAAWG 797

BLAST of Tan0014479 vs. ExPASy TrEMBL
Match: A0A1S3C6H6 (uncharacterized protein LOC103497596 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103497596 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 2.6e-39
Identity = 95/210 (45.24%), Postives = 131/210 (62.38%), Query Frame = 0

Query: 181 EIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTYKISELWMNN 240
           EI +SIVYGGL E I SLGIVTSAA+  T T  IV +S+A+LI+GLFI+ + ++ L    
Sbjct: 527 EIVKSIVYGGLAESITSLGIVTSAASANTGTGNIVVLSLANLISGLFILGHNLTGLKSEQ 586

Query: 241 DKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFTFRKPEDKLL 300
            ++  +  +    +RYE + G+++NY LHF +AI SFV FGLVPPLVYGF+F K  DK L
Sbjct: 587 FRTSNETDDDDHVDRYEVVLGNRENYILHFVLAIFSFVLFGLVPPLVYGFSFTKSNDKDL 646

Query: 301 KISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLTTLGGDLIENY 360
           K++ V   ++ C  LL +GKAYI+RP+    YI  +   IV A G    + L G+LI+  
Sbjct: 647 KLTAVAGASLLCITLLALGKAYIQRPNRWDVYIKTVVSYIVIAAGAGGFSYLAGNLIDKC 706

Query: 361 IQQIGWFEPNKIPTLFQPGLSLSFENGTWG 391
           +++ GWFE N    L  P   +S     WG
Sbjct: 707 VKKYGWFEENPAFNLGLPLPEMSLVKAAWG 736

BLAST of Tan0014479 vs. TAIR 10
Match: AT4G27860.1 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 123.6 bits (309), Expect = 3.5e-28
Identity = 78/210 (37.14%), Postives = 123/210 (58.57%), Query Frame = 0

Query: 168 SAIPQPSGSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLF 227
           S  P+  G+  K+EI +SIVYGGLTE I SL  VTSAAA+  +T  ++A+ +A+L +GL 
Sbjct: 401 SVEPRKGGN--KVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLL 460

Query: 228 IMTYKISELWMNNDKSRED-------EAEAKEEERYENIFGDKKNYRLHFTVAILSFVCF 287
           +  + + EL   N+K R+        E    EE+RYE + G ++  R+H  +AI SFV F
Sbjct: 461 LTVHSLQEL--INEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIF 520

Query: 288 GLVPPLVYGFTFRKPEDKL--LKISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGK 347
           GL+PPLVYGF+FRK  +K    K+  V  V++ C  LL + KAY+ +     +Y+  + +
Sbjct: 521 GLIPPLVYGFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKR---DYVKTLFR 580

Query: 348 CIVDAIGTSVLTTLGGDLIENYIQQIGWFE 369
               A   S  +   G L+  ++++ G+++
Sbjct: 581 YTTTATTASGFSQFVGYLVSQWLEKSGFYD 603

BLAST of Tan0014479 vs. TAIR 10
Match: AT4G27860.2 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 123.6 bits (309), Expect = 3.5e-28
Identity = 78/210 (37.14%), Postives = 123/210 (58.57%), Query Frame = 0

Query: 168 SAIPQPSGSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLF 227
           S  P+  G+  K+EI +SIVYGGLTE I SL  VTSAAA+  +T  ++A+ +A+L +GL 
Sbjct: 386 SVEPRKGGN--KVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLL 445

Query: 228 IMTYKISELWMNNDKSRED-------EAEAKEEERYENIFGDKKNYRLHFTVAILSFVCF 287
           +  + + EL   N+K R+        E    EE+RYE + G ++  R+H  +AI SFV F
Sbjct: 446 LTVHSLQEL--INEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIF 505

Query: 288 GLVPPLVYGFTFRKPEDKL--LKISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGK 347
           GL+PPLVYGF+FRK  +K    K+  V  V++ C  LL + KAY+ +     +Y+  + +
Sbjct: 506 GLIPPLVYGFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKR---DYVKTLFR 565

Query: 348 CIVDAIGTSVLTTLGGDLIENYIQQIGWFE 369
               A   S  +   G L+  ++++ G+++
Sbjct: 566 YTTTATTASGFSQFVGYLVSQWLEKSGFYD 588

BLAST of Tan0014479 vs. TAIR 10
Match: AT5G24290.2 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.9e-26
Identity = 66/197 (33.50%), Postives = 117/197 (59.39%), Query Frame = 0

Query: 172 QPSGSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTY 231
           QP G    IE+ +S VYGGLTE I SLG+V+SA+A+ ++T  I+A+++A+L  GL ++  
Sbjct: 320 QPDGERFAIELLKSTVYGGLTETITSLGVVSSASASGSSTMNILALAVANLAGGLIVLAQ 379

Query: 232 KISELWMNNDKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFT 291
              +L  ++D         +E++RYE + G +   R+H  VA++S++ FGL+PPLVY F+
Sbjct: 380 NFQDLRNSSD---------QEKDRYEELLGRRTKSRIHILVAVMSYIFFGLIPPLVYAFS 439

Query: 292 FRKPEDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCV----EYINIIGKCIVDAIGTS 351
           F +   K  K+ +V + ++ C  LLG  K Y+++P+N       Y+          + + 
Sbjct: 440 FYETGIKNYKLISVFLGSLVCVILLGSIKVYVRKPTNSCGSTKAYLKSAAYYTSIVVASC 499

Query: 352 VLTTLGGDLIENYIQQI 365
            ++ + GD++  YI+++
Sbjct: 500 GISYVVGDIMGEYIEKL 507

BLAST of Tan0014479 vs. TAIR 10
Match: AT5G24290.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.9e-26
Identity = 66/197 (33.50%), Postives = 117/197 (59.39%), Query Frame = 0

Query: 172 QPSGSIIKIEIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTY 231
           QP G    IE+ +S VYGGLTE I SLG+V+SA+A+ ++T  I+A+++A+L  GL ++  
Sbjct: 336 QPDGERFAIELLKSTVYGGLTETITSLGVVSSASASGSSTMNILALAVANLAGGLIVLAQ 395

Query: 232 KISELWMNNDKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFT 291
              +L  ++D         +E++RYE + G +   R+H  VA++S++ FGL+PPLVY F+
Sbjct: 396 NFQDLRNSSD---------QEKDRYEELLGRRTKSRIHILVAVMSYIFFGLIPPLVYAFS 455

Query: 292 FRKPEDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCV----EYINIIGKCIVDAIGTS 351
           F +   K  K+ +V + ++ C  LLG  K Y+++P+N       Y+          + + 
Sbjct: 456 FYETGIKNYKLISVFLGSLVCVILLGSIKVYVRKPTNSCGSTKAYLKSAAYYTSIVVASC 515

Query: 352 VLTTLGGDLIENYIQQI 365
            ++ + GD++  YI+++
Sbjct: 516 GISYVVGDIMGEYIEKL 523

BLAST of Tan0014479 vs. TAIR 10
Match: AT4G27870.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 113.2 bits (282), Expect = 4.7e-25
Identity = 80/258 (31.01%), Postives = 139/258 (53.88%), Query Frame = 0

Query: 121 VVGEQSSGNCSVITNQNLATEDQATTNTDTETIQTQTLVRREEMVLVSAIPQPSGSIIKI 180
           V G   +G+  VIT       D +  N   E    + L+RR   V+V           K+
Sbjct: 495 VTGVMDTGDRGVITGPANPEIDISAGNLLEEGSLREPLMRR---VVVQG--------RKL 554

Query: 181 EIARSIVYGGLTELIASLGIVTSAAATTTTTRKIVAVSIASLITGLFIMTYKISEL---- 240
           EI +SIVYGGL E I SLG+++SAA +  +   I+ + +A+L+ GL ++ + + EL    
Sbjct: 555 EILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGGLILIIHNLQELREEE 614

Query: 241 -WMNNDKSREDEAEAKEEERYENIFGDKKNYRLHFTVAILSFVCFGLVPPLVYGFTFRKP 300
                 +  +     +EE RY+ + G ++N+ LH TVAILSF+  G++PP+VY F+F + 
Sbjct: 615 PIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFSFSEK 674

Query: 301 EDKLLKISTVTIVAVFCTGLLGVGKAYIKRPSNCVEYINIIGKCIVDAIGTSVLTTLGGD 360
            +K  K+++V   ++FC  LL + KA+++ P     Y+  I      A+  S ++ + G+
Sbjct: 675 HNKDYKVASVFGASLFCIVLLAIAKAHVRYPRG--SYLKSILYYGSIAVSVSGISYVVGN 734

Query: 361 LIENYIQQIGWFEPNKIP 374
            +E  +++ GW + ++ P
Sbjct: 735 FLEQLLEKHGWSDGSETP 739

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W4P84.9e-2737.14Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1[more]
F4KFS72.7e-2533.50Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1[more]
Q8LPT36.6e-2431.01Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL... [more]
Match NameE-valueIdentityDescription
XP_038897302.13.3e-8958.36membrane protein of ER body 1-like isoform X3 [Benincasa hispida][more]
XP_038897303.19.0e-8757.77uncharacterized protein LOC120085412 isoform X4 [Benincasa hispida][more]
XP_038897300.12.9e-8554.67membrane protein of ER body-like protein isoform X1 [Benincasa hispida][more]
XP_038897301.12.9e-8554.67membrane protein of ER body-like protein isoform X2 [Benincasa hispida][more]
XP_022144855.16.7e-8253.82membrane protein of ER body 2-like isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1CSU13.2e-8253.82membrane protein of ER body 2-like isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1JX701.3e-5952.26membrane protein of ER body 1-like OS=Cucurbita maxima OX=3661 GN=LOC111488291 P... [more]
A0A5A7SK092.6e-3945.24Membrane protein of ER body-like protein isoform X4 OS=Cucumis melo var. makuwa ... [more]
A0A5D3CME82.6e-3945.24Membrane protein of ER body-like protein isoform X4 OS=Cucumis melo var. makuwa ... [more]
A0A1S3C6H62.6e-3945.24uncharacterized protein LOC103497596 isoform X3 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT4G27860.13.5e-2837.14vacuolar iron transporter (VIT) family protein [more]
AT4G27860.23.5e-2837.14vacuolar iron transporter (VIT) family protein [more]
AT5G24290.21.9e-2633.50Vacuolar iron transporter (VIT) family protein [more]
AT5G24290.11.9e-2633.50Vacuolar iron transporter (VIT) family protein [more]
AT4G27870.14.7e-2531.01Vacuolar iron transporter (VIT) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 64..93
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 64..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..42
NoneNo IPR availablePANTHERPTHR31851:SF64GLYCINE-RICH PROTEIN FAMILYcoord: 105..378
IPR008217Ccc1 familyPANTHERPTHR31851FE(2+)/MN(2+) TRANSPORTER PCL1coord: 105..378

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0014479.1Tan0014479.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030026 cellular manganese ion homeostasis
biological_process GO:0051179 localization
cellular_component GO:0016020 membrane
molecular_function GO:0005384 manganese ion transmembrane transporter activity