Tan0014354 (gene) Snake gourd v1

Overview


Name	Tan0014354
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	rRNA N-glycosidase
Location	LG06: 29863221 .. 29864057 (+)
RNA-Seq Expression	Tan0014354
Synteny	Tan0014354

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAGATTCTCAATCCTTTGTTTGCTAATTCTGGTGATCTTCCATGGAGGTCGCCGTGTTGAAGGCGATAATATCAACTTCAGTCTGTCAGGTTCCAATTCAAAATCTTATAGCAAATTCATAACATCTTTAAGAAACGCTCTCCCAAAAGCAGGAAAAGTGTTCGATATACCCATATTGCTTCCCACTGCTTCGGGCTCAAGCCGCTACAAACTCATGCAACTCTCTAATTATGAAGATAAAACCATCACATTGGCCGTAGATGTAACAAACGTTTATCTTATGGGGTATCTTGTCAATACAACATCCTACTTTTTCAACGAGTCTGATGCTCAATTAGCTTCTAAATTTGTATTCAAAGGTAGTAAGATTATTACACTTCCATATTCTGGTAATTACCAAAGGCTTCAAATTGCTGCAGGCAAAGAAAGAGACTCTATTCCCCTTGGATTCCTAGCCTTGGACACTGCCATTTCCTCCTTGTATCATTATGATTCTAGAGCTGCTGCCGCCGCATTTCTCGTAATCATTCAAACCACTGCCGAGGCTTCAAGATTTAAATATATTGAGAAGCAAATTATCGAGAGAATTCATAAAAACGAGGTGCCAAGTCTTGCAGCTATTAGTTTGGAAAACGAATGGTCTATTCTCTCCAAACAAATTCAGATTGCTAACTCTAATAACGGAATATTTCAAACTCCCGTCAAGCTTGTTAACGATAAAGGCCATGTAGTTGAAATTACTAACACTAGTTCTCTCGTGGTCAGCAACAACATTAAGCTACTGCTAAACAAGCAAAATATGGCAGCTTTTCAAGACGATATTTCTACATGA

mRNA sequence

ATGAATAGATTCTCAATCCTTTGTTTGCTAATTCTGGTGATCTTCCATGGAGGTCGCCGTGTTGAAGGCGATAATATCAACTTCAGTCTGTCAGGTTCCAATTCAAAATCTTATAGCAAATTCATAACATCTTTAAGAAACGCTCTCCCAAAAGCAGGAAAAGTGTTCGATATACCCATATTGCTTCCCACTGCTTCGGGCTCAAGCCGCTACAAACTCATGCAACTCTCTAATTATGAAGATAAAACCATCACATTGGCCGTAGATGTAACAAACGTTTATCTTATGGGGTATCTTGTCAATACAACATCCTACTTTTTCAACGAGTCTGATGCTCAATTAGCTTCTAAATTTGTATTCAAAGGTAGTAAGATTATTACACTTCCATATTCTGGTAATTACCAAAGGCTTCAAATTGCTGCAGGCAAAGAAAGAGACTCTATTCCCCTTGGATTCCTAGCCTTGGACACTGCCATTTCCTCCTTGTATCATTATGATTCTAGAGCTGCTGCCGCCGCATTTCTCGTAATCATTCAAACCACTGCCGAGGCTTCAAGATTTAAATATATTGAGAAGCAAATTATCGAGAGAATTCATAAAAACGAGGTGCCAAGTCTTGCAGCTATTAGTTTGGAAAACGAATGGTCTATTCTCTCCAAACAAATTCAGATTGCTAACTCTAATAACGGAATATTTCAAACTCCCGTCAAGCTTGTTAACGATAAAGGCCATGTAGTTGAAATTACTAACACTAGTTCTCTCGTGGTCAGCAACAACATTAAGCTACTGCTAAACAAGCAAAATATGGCAGCTTTTCAAGACGATATTTCTACATGA

Coding sequence (CDS)

ATGAATAGATTCTCAATCCTTTGTTTGCTAATTCTGGTGATCTTCCATGGAGGTCGCCGTGTTGAAGGCGATAATATCAACTTCAGTCTGTCAGGTTCCAATTCAAAATCTTATAGCAAATTCATAACATCTTTAAGAAACGCTCTCCCAAAAGCAGGAAAAGTGTTCGATATACCCATATTGCTTCCCACTGCTTCGGGCTCAAGCCGCTACAAACTCATGCAACTCTCTAATTATGAAGATAAAACCATCACATTGGCCGTAGATGTAACAAACGTTTATCTTATGGGGTATCTTGTCAATACAACATCCTACTTTTTCAACGAGTCTGATGCTCAATTAGCTTCTAAATTTGTATTCAAAGGTAGTAAGATTATTACACTTCCATATTCTGGTAATTACCAAAGGCTTCAAATTGCTGCAGGCAAAGAAAGAGACTCTATTCCCCTTGGATTCCTAGCCTTGGACACTGCCATTTCCTCCTTGTATCATTATGATTCTAGAGCTGCTGCCGCCGCATTTCTCGTAATCATTCAAACCACTGCCGAGGCTTCAAGATTTAAATATATTGAGAAGCAAATTATCGAGAGAATTCATAAAAACGAGGTGCCAAGTCTTGCAGCTATTAGTTTGGAAAACGAATGGTCTATTCTCTCCAAACAAATTCAGATTGCTAACTCTAATAACGGAATATTTCAAACTCCCGTCAAGCTTGTTAACGATAAAGGCCATGTAGTTGAAATTACTAACACTAGTTCTCTCGTGGTCAGCAACAACATTAAGCTACTGCTAAACAAGCAAAATATGGCAGCTTTTCAAGACGATATTTCTACATGA

Protein sequence

MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPILLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVFKGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQTTAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVNDKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQDDIST

Homology

[Close]

BLAST of Tan0014354 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 3.6e-109
Identity = 204/278 (73.38%), Postives = 241/278 (86.69%), Query Frame = 0


Query: 1   MNRFSIL-CLLILVIFHGGRRVEGD-NINFSLSGSNSKSYSKFITSLRNALPKAGKVFDI 60
           MNRFS+L CL+IL IFHG    EGD  + FSL GSN KSYSKFITS+RNALP AG +++I
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYNI 60

Query: 61  PILLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKF 120
           P+L+P+ SGS RY LMQLSNYE  TIT+AVDVTNVY+MGYLVN TSYFFNE+DAQLASKF
Sbjct: 61  PLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASKF 120

Query: 121 VFKGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVII 180
           VF+G+K ITLPYSGNYQ+LQ  A KERDSIPLGF+ALD+AIS+LY+YDSR+A  AFLV+I
Sbjct: 121 VFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVLI 180

Query: 181 QTTAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKL 240
           QTTAEA+R+KYIEKQII+RI  ++VP LAAISLENEWS+LSKQIQIA SNNG FQTPVK+
Sbjct: 181 QTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVKI 240

Query: 241 VNDKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQDDI 277
           +NDKG + E+TN SSLVV+ NI LLLNK N+A+F+D +
Sbjct: 241 INDKGILTEVTNVSSLVVTKNIMLLLNKLNIASFEDHV 278

[Close]

BLAST of Tan0014354 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 2.0e-99
Identity = 195/278 (70.14%), Postives = 231/278 (83.09%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           M RF++   LIL IF     VE D + FSLSGS+S SYSKFI  LR ALP  G V++I +
Sbjct: 1   MKRFTV---LILAIFVAASTVEAD-VRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITL 60

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LL +ASG+SRY LM LSNY+ K IT+AVDVTNVY+MGYLVN+TSYFFNESDA+LAS++VF
Sbjct: 61  LLSSASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQT 180
           KGS I+TLPYSGNY++LQ AAGK R+ IPLGF ALD+AI++L+HYDS AAAAAFLVIIQT
Sbjct: 121 KGSTIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQT 180

Query: 181 TAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVN 240
           TAEASRFKYIE QIIERI KN+VPSLA ISLENEWS LSKQIQ+A +NNG F+TPV + +
Sbjct: 181 TAEASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITD 240

Query: 241 DKGHVVEITNTSSLVVSNNIKLLLN-KQNMAAFQDDIS 278
           DKG  VEITN +S VV+ NI+LLLN KQN+AAF +D+S
Sbjct: 241 DKGQRVEITNVTSKVVTKNIQLLLNYKQNVAAFDEDVS 274

[Close]

BLAST of Tan0014354 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.3e-92
Identity = 178/249 (71.49%), Postives = 217/249 (87.15%), Query Frame = 0


Query: 25  NINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPILLPTASGSSRYKLMQLSNYEDKTI 84
           N++FSLSG++SKSYSKFIT+LR ALP   KV +IP+LLP+ASG+SRY LMQLSNY+ K I
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVFKGSKIITLPYSGNYQRLQIAAGKE 144
           T+A+DVTNVY+MGYLVN+TSYF NESDA+LAS++VFKGS ++T+PYSGNY+RLQ AAGK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKI 121

Query: 145 RDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQTTAEASRFKYIEKQIIERIHKNEVP 204
           R+ IPLGF ALD+A++S++HYDS AAAAAFLVI+QTTAEASRFKYIE QIIERI KNEVP
Sbjct: 122 REKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEVP 181

Query: 205 SLAAISLENE-WSILSKQIQIANSNNGIFQTPVKLVNDKGHVVEITNTSSLVVSN--NIK 264
           S AA+SLENE WS+LSKQIQ+A +NNG F+TPV ++++KG  VEITN +S V     N K
Sbjct: 182 SPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNSK 241

Query: 265 LLLNKQNMA 271
           LLLNKQN+A
Sbjct: 242 LLLNKQNIA 250

[Close]

BLAST of Tan0014354 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 336.3 bits (861), Expect = 3.4e-91
Identity = 179/280 (63.93%), Postives = 228/280 (81.43%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           M+RFS+L  LIL IF GG  V+GD ++F LSG++ +SY  FI  LRNALP   KV++IP+
Sbjct: 1   MSRFSVLSFLILAIFLGGSIVKGD-VSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LLP+ SG+ RY LM L NY+ KTIT+AVDVTNVY+MGYL +TTSYFFNE  A+LAS++VF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KGS-KIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQ 180
           + + + ITLPYSGNY+RLQIAAGK R+ IP+G  ALD+AIS+L HYDS AAA A LV+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 TTAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLV 240
           TTAEA+RFKYIE+QI ER +++EVPSLA ISLEN WS LSKQIQ+A  NNGIF+TP+ LV
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 NDKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQD-DIST 279
           ++KG+ V+ITN +S VV++NI+LLLN +N+A   + D+ST
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIAEGDNGDVST 279

[Close]

BLAST of Tan0014354 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 325.9 bits (834), Expect = 4.6e-88
Identity = 169/241 (70.12%), Postives = 205/241 (85.06%), Query Frame = 0


Query: 25  NINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPILLPTASGSSRYKLMQLSNYEDKTI 84
           +++FSLSGS+S SYSKFI +LR ALP  G V++I +LL +ASG+SRY LM+LSNY+ K I
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 85  TLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVFKGSKIITLPYSGNYQRLQIAAGKE 144
           T+A+DVTNVY+MGYLVN+TSYFFNESDA+LAS++VF GS I+TLPYSGNY++LQ AAGK 
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 145 RDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQTTAEASRFKYIEKQIIERIHKNEVP 204
           R+ IPLGF ALD+AI++L+HYDS AAAAAFLVIIQTTAE+SRFKYIE QII RI KN VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 205 SLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVNDKGHVVEITNTSSLVVSNNIKLLL 264
           SLA ISLENEWS LSKQIQ+A +NNG F+TPV +++  G  VEI N  S VV+ NI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 265 N 266
           N
Sbjct: 241 N 241

[Close]

BLAST of Tan0014354 vs. NCBI nr
Match: XP_023547652.1 (putative ribosome-inactivating protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 427.9 bits (1099), Expect = 6.5e-116
Identity = 219/278 (78.78%), Postives = 249/278 (89.57%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           MNR S+L LLIL IFH G    GDN++FSLSGS+SKSYSKFITSLRNALP AGKV++IP+
Sbjct: 1   MNRLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPL 60

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LLPT  GS+RY LM+LSNYE+KTIT+A+DVTNVY+MGYLVNTTSYFFNE  AQLAS+FVF
Sbjct: 61  LLPTLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVF 120

Query: 121 KGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQT 180
           + +K I LPYSGNYQ+LQIAA K+RDSIPLGFLALDTAIS+LYHYD++AA AAFLV+IQT
Sbjct: 121 QDTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQT 180

Query: 181 TAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVN 240
           TAEASRFKYIEKQII+RI +NEVPSLAAISLENEWS+LSKQIQIANSNNG FQTPVKL+N
Sbjct: 181 TAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIN 240

Query: 241 DKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQDDIST 279
           DKG  VE+TN SSLVV+NNIKLLLNKQN+A F+    T
Sbjct: 241 DKGLSVEVTNVSSLVVTNNIKLLLNKQNVATFKPQAIT 278

[Close]

BLAST of Tan0014354 vs. NCBI nr
Match: XP_022953187.1 (putative ribosome-inactivating protein [Cucurbita moschata])

HSP 1 Score: 427.2 bits (1097), Expect = 1.1e-115
Identity = 218/278 (78.42%), Postives = 249/278 (89.57%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           MNR S+L LLIL IFH G    GDN++FSLSGS+SKSYSKFITSLRNALP AGKV++IP+
Sbjct: 1   MNRLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPL 60

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LLP   GS+RY LM+LSNYE+KTIT+A+DVTNVY+MGYLVNTTSYFFNE  AQLAS+FVF
Sbjct: 61  LLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVF 120

Query: 121 KGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQT 180
           +G+K I LPYSGNYQ+LQIAA K+RDSIPLGFLALDTAIS+LYHYD++AA AAFLV+IQT
Sbjct: 121 QGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQT 180

Query: 181 TAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVN 240
           TAEASRFKYIEKQII+RI +NEVPSLAAISLENEWS+LSKQIQIANSNNG FQTPVKL+N
Sbjct: 181 TAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIN 240

Query: 241 DKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQDDIST 279
           DKG  VE+TN SSLVV+NNIKLLLNKQN+A  + +  T
Sbjct: 241 DKGLSVEVTNVSSLVVTNNIKLLLNKQNVATIKPEAIT 278

[Close]

BLAST of Tan0014354 vs. NCBI nr
Match: KAG6575377.1 (hypothetical protein SDJN03_26016, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013918.1 hypothetical protein SDJN02_24087, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 417.5 bits (1072), Expect = 8.8e-113
Identity = 217/278 (78.06%), Postives = 248/278 (89.21%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           MNR S+L LLIL IFH G    GDN++FSLSGS+SKSYSKFITSLRNALP AGKV++IP+
Sbjct: 1   MNRLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPL 60

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LLPT  GS+RY LM+LSNYE+KTIT+A+DVTNVY+MGYLVNTTSYFFNE  AQLAS+FVF
Sbjct: 61  LLPTLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVF 120

Query: 121 KGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQT 180
           +G+K I LPYSGNYQ+LQIAA K+RDSIPLGFLALDTAIS+LYHYD++AA AAFLV+IQT
Sbjct: 121 QGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQT 180

Query: 181 TAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVN 240
           TAEASRFKYIEKQII+RI +NEVPSLAAISLE   S+LSKQIQIANSNNG FQTPVKL+N
Sbjct: 181 TAEASRFKYIEKQIIDRIGRNEVPSLAAISLER--SLLSKQIQIANSNNGTFQTPVKLIN 240

Query: 241 DKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQDDIST 279
           DKG  VE+TN SSLVV+NNIKLLLNKQN+A F+ +  T
Sbjct: 241 DKGLSVEVTNVSSLVVTNNIKLLLNKQNVATFKPEAIT 276

[Close]

BLAST of Tan0014354 vs. NCBI nr
Match: Q9FRX4.1 (RecName: Full=Putative ribosome-inactivating protein; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Cucumis ficifolius] >BAB19677.1 unnamed protein product [Cucumis ficifolius])

HSP 1 Score: 396.0 bits (1016), Expect = 2.7e-106
Identity = 204/278 (73.38%), Postives = 241/278 (86.69%), Query Frame = 0


Query: 1   MNRFSIL-CLLILVIFHGGRRVEGD-NINFSLSGSNSKSYSKFITSLRNALPKAGKVFDI 60
           MNRFS+L CL+IL IFHG    EGD  + FSL GSN KSYSKFITS+RNALP AG +++I
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYNI 60

Query: 61  PILLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKF 120
           P+L+P+ SGS RY LMQLSNYE  TIT+AVDVTNVY+MGYLVN TSYFFNE+DAQLASKF
Sbjct: 61  PLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASKF 120

Query: 121 VFKGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVII 180
           VF+G+K ITLPYSGNYQ+LQ  A KERDSIPLGF+ALD+AIS+LY+YDSR+A  AFLV+I
Sbjct: 121 VFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVLI 180

Query: 181 QTTAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKL 240
           QTTAEA+R+KYIEKQII+RI  ++VP LAAISLENEWS+LSKQIQIA SNNG FQTPVK+
Sbjct: 181 QTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVKI 240

Query: 241 VNDKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQDDI 277
           +NDKG + E+TN SSLVV+ NI LLLNK N+A+F+D +
Sbjct: 241 INDKGILTEVTNVSSLVVTKNIMLLLNKLNIASFEDHV 278

[Close]

BLAST of Tan0014354 vs. NCBI nr
Match: ABY71834.1 (ribosome-inactivating protein [Luffa acutangula] >CAA44230.1 b-luffin [Luffa aegyptiaca])

HSP 1 Score: 380.9 bits (977), Expect = 9.1e-102
Identity = 200/278 (71.94%), Postives = 238/278 (85.61%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           MNRF+ L LLI++I      VEG N++FSLSG++SKSYSKFIT+LR ALP   KV +IP+
Sbjct: 1   MNRFTFLSLLIILI--AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LLP+ASG+SRY LMQLSNY+ K IT+A+DVTNVY+MGYLVN+TSYFFNESDA+LAS++VF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQT 180
           KGS I+TLPYSGNY+RLQ AAGK R+ IPLGF A D+AI+SL+HYDS AAA AFLVIIQT
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVN 240
           TAEASRFKYIE QIIERI KNEVPS AA+SLENEWS LSKQIQ+A +NNG F+TPV +++
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQDDIST 279
           +KG  VEI + +S VV+NNIKLLLNKQN+AAF D I T
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPT 276

[Close]

BLAST of Tan0014354 vs. ExPASy TrEMBL
Match: A0A6J1GMJ7 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455802 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 5.4e-116
Identity = 218/278 (78.42%), Postives = 249/278 (89.57%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           MNR S+L LLIL IFH G    GDN++FSLSGS+SKSYSKFITSLRNALP AGKV++IP+
Sbjct: 1   MNRLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPL 60

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LLP   GS+RY LM+LSNYE+KTIT+A+DVTNVY+MGYLVNTTSYFFNE  AQLAS+FVF
Sbjct: 61  LLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVF 120

Query: 121 KGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQT 180
           +G+K I LPYSGNYQ+LQIAA K+RDSIPLGFLALDTAIS+LYHYD++AA AAFLV+IQT
Sbjct: 121 QGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQT 180

Query: 181 TAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVN 240
           TAEASRFKYIEKQII+RI +NEVPSLAAISLENEWS+LSKQIQIANSNNG FQTPVKL+N
Sbjct: 181 TAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIN 240

Query: 241 DKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQDDIST 279
           DKG  VE+TN SSLVV+NNIKLLLNKQN+A  + +  T
Sbjct: 241 DKGLSVEVTNVSSLVVTNNIKLLLNKQNVATIKPEAIT 278

[Close]

BLAST of Tan0014354 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 4.4e-102
Identity = 200/278 (71.94%), Postives = 238/278 (85.61%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           MNRF+ L LLI++I      VEG N++FSLSG++SKSYSKFIT+LR ALP   KV +IP+
Sbjct: 1   MNRFTFLSLLIILI--AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LLP+ASG+SRY LMQLSNY+ K IT+A+DVTNVY+MGYLVN+TSYFFNESDA+LAS++VF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQT 180
           KGS I+TLPYSGNY+RLQ AAGK R+ IPLGF A D+AI+SL+HYDS AAA AFLVIIQT
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVN 240
           TAEASRFKYIE QIIERI KNEVPS AA+SLENEWS LSKQIQ+A +NNG F+TPV +++
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQDDIST 279
           +KG  VEI + +S VV+NNIKLLLNKQN+AAF D I T
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPT 276

[Close]

BLAST of Tan0014354 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 4.4e-102
Identity = 200/278 (71.94%), Postives = 238/278 (85.61%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           MNRF+ L LLI++I      VEG N++FSLSG++SKSYSKFIT+LR ALP   KV +IP+
Sbjct: 1   MNRFTFLSLLIILI--AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LLP+ASG+SRY LMQLSNY+ K IT+A+DVTNVY+MGYLVN+TSYFFNESDA+LAS++VF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQT 180
           KGS I+TLPYSGNY+RLQ AAGK R+ IPLGF A D+AI+SL+HYDS AAA AFLVIIQT
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVN 240
           TAEASRFKYIE QIIERI KNEVPS AA+SLENEWS LSKQIQ+A +NNG F+TPV +++
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DKGHVVEITNTSSLVVSNNIKLLLNKQNMAAFQDDIST 279
           +KG  VEI + +S VV+NNIKLLLNKQN+AAF D I T
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPT 276

[Close]

BLAST of Tan0014354 vs. ExPASy TrEMBL
Match: A0A6J1JVG7 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.6e-91
Identity = 176/271 (64.94%), Postives = 223/271 (82.29%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           MN  S+ CL+IL IFHG       +++F+L GSNSKSYS FI +LR+ALP   KV++I +
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 70

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LL +A+G+SRY  ++LSNY+ K IT+A+DVTNVY+MGYLVN+TSYFFNESDAQLASK+VF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQT 180
           K S  ITLPYSGNY++LQ AAGK+R+ IPLGF ALD+AI++L+HYDS AAAAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVN 240
           TAEASR+KYIE Q+I+RI K++VPS A ISLEN WS LSKQIQ+A +N G+F+ PV + +
Sbjct: 191 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DKGHVVEITNTSSLVVSNNIKLLLNKQNMAA 272
           D+G  VEITN SS VV+ NI+LLLN QN+AA
Sbjct: 251 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAA 281

[Close]

BLAST of Tan0014354 vs. ExPASy TrEMBL
Match: A0A6J1GMM2 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.7e-91
Identity = 174/270 (64.44%), Postives = 222/270 (82.22%), Query Frame = 0


Query: 1   MNRFSILCLLILVIFHGGRRVEGDNINFSLSGSNSKSYSKFITSLRNALPKAGKVFDIPI 60
           MN  S+ CL+IL IFHG       +++F+L GSNSKSYS FI +LR+ALP   KV++I +
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLPTASGSSRYKLMQLSNYEDKTITLAVDVTNVYLMGYLVNTTSYFFNESDAQLASKFVF 120
           LL +A+G+SRY  ++LSNY+ K IT+A+DVTNVY+MGYLVN+TSYFFNESDAQLASK+VF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KGSKIITLPYSGNYQRLQIAAGKERDSIPLGFLALDTAISSLYHYDSRAAAAAFLVIIQT 180
           K S  ITLPYSGNY++LQ AAGK+R+ IPLGF ALD AI++L+HYDS AAAAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRFKYIEKQIIERIHKNEVPSLAAISLENEWSILSKQIQIANSNNGIFQTPVKLVN 240
           TAEASR++YIE Q+I+RI K++VPSLA ISLEN WS LSKQIQ+A +N G+F+ PV + +
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DKGHVVEITNTSSLVVSNNIKLLLNKQNMA 271
           D+G  VEITN SS VV+ NI+LLLN +N+A
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 280

The following BLAST results are available for this feature:

BLAST of Tan0014354 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FRX4	3.6e-109	73.38	Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1	[more]
Q00465	2.0e-99	70.14	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P22851	2.3e-92	71.49	Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P16094	3.4e-91	63.93	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
P84530	4.6e-88	70.12	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=...	[more]

BLAST of Tan0014354 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023547652.1	6.5e-116	78.78	putative ribosome-inactivating protein [Cucurbita pepo subsp. pepo]	[more]
XP_022953187.1	1.1e-115	78.42	putative ribosome-inactivating protein [Cucurbita moschata]	[more]
KAG6575377.1	8.8e-113	78.06	hypothetical protein SDJN03_26016, partial [Cucurbita argyrosperma subsp. sorori...	[more]
Q9FRX4.1	2.7e-106	73.38	RecName: Full=Putative ribosome-inactivating protein; AltName: Full=rRNA N-glyco...	[more]
ABY71834.1	9.1e-102	71.94	ribosome-inactivating protein [Luffa acutangula] >CAA44230.1 b-luffin [Luffa aeg...	[more]

BLAST of Tan0014354 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMJ7	5.4e-116	78.42	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455802 PE=3 SV=1	[more]
B0EVM6	4.4e-102	71.94	rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1	[more]
Q00980	4.4e-102	71.94	rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1JVG7	1.6e-91	64.94	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1	[more]
A0A6J1GMM2	2.7e-91	64.44	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1	[more]

BLAST of Tan0014354 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 174..194 score: 57.64 coord: 36..49 score: 46.43 coord: 145..159 score: 50.7 coord: 206..223 score: 51.17 coord: 83..98 score: 54.93
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 186..270 e-value: 9.1E-27 score: 94.8
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 35..223 e-value: 3.0E-32 score: 111.8
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 27..269
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..185 e-value: 3.6E-45 score: 155.5
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 27..269
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 178..194
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 26..268

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0014354.1	Tan0014354.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051607	defense response to virus
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity