Tan0014327 (gene) Snake gourd v1

BLAST of Tan0014327 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.1e-70
Identity = 142/328 (43.29%), Postives = 205/328 (62.50%), Query Frame = 0

Query: 5   KFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDASKEFG 64
           K +S      ++PE+YV P   RP    V   +  P++DLS+ DR+ L+++I  A   FG
Sbjct: 4   KLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFG 63

Query: 65  FFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKEQTHY 124
           FFQVINHGV+K++++E   V +EF +MS  EK K  S DP K+ R+ TS  N  KE+ + 
Sbjct: 64  FFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNN 123

Query: 125 WRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLEREYLG 184
           WRD L  +C+P+ KYV+ WP NPP ++E+V  Y   +R++  +I ELISE L LE++Y+ 
Sbjct: 124 WRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMK 183

Query: 185 GEMSENPK-LLVNHYPPCPNPSLTLGLNQHCDPSLITILFQE--VNGLQVLKDGQWIGVH 244
             + E  + + VN+YPPCP P LT GL  H DP+ +TIL Q+  V GLQ+L DGQW  V+
Sbjct: 184 KVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVN 243

Query: 245 PIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCL-- 304
           P  +AFV+NIG  LQ +SNG+ K+V HRAVTN++  R S+A  + P D  +M PAK L  
Sbjct: 244 PHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWE 303

Query: 305 -INEANPPHYRSLMFKDFQTNYVPRAAD 327
             ++   P Y+   + ++   +  R  D
Sbjct: 304 AEDDETKPVYKDFTYAEYYKKFWSRNLD 330

BLAST of Tan0014327 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.9e-67
Identity = 136/325 (41.85%), Postives = 191/325 (58.77%), Query Frame = 0

Query: 14  DSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDASKEFGFFQVINHGV 73
           D++P  YV P  +RP   +V  +  IPVVDL++ DRA +V  + DA +  GFFQV+NHG+
Sbjct: 18  DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77

Query: 74  SKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKEQTHYWRDALVFNC 133
              ++     V +EF  +   EKAK  S DP K  R+ TS  N  KE  H WRD L  +C
Sbjct: 78  DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHC 137

Query: 134 HPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLEREYLGGEMSENPK- 193
           +PL ++V  WP NPP ++E++G YC  +R+L   + E ISE L LE  Y+   + E  + 
Sbjct: 138 YPLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQH 197

Query: 194 LLVNHYPPCPNPSLTLGLNQHCDPSLITILF--QEVNGLQVLKDGQWIGVHPIDNAFVVN 253
           + VN+YP CP P LT GL  H DP+ +TIL    +V GLQVL DG+WI V+P   A V+N
Sbjct: 198 MAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVIN 257

Query: 254 IGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLINEANPPHYRS 313
           IG  LQ +SNG  ++V HRAV NS   R S+A  + P + V + PAK LI + +P  YR+
Sbjct: 258 IGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRN 317

Query: 314 LMFKDFQTNYVPRAADTNAVMQYIR 336
             + ++   +  R  D    ++  R
Sbjct: 318 YTYDEYYKKFWSRNLDQEHCLELFR 341

BLAST of Tan0014327 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.3e-65
Identity = 139/332 (41.87%), Postives = 194/332 (58.43%), Query Frame = 0

Query: 6   FVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDASKEFGF 65
           FVSNW +  SV ES++ P +KR     VP+   +P++DL      LLV++I  A ++FG 
Sbjct: 4   FVSNW-STKSVSESFIAPLQKR-AEKDVPVGNDVPIIDLQ-QHHHLLVQQITKACQDFGL 63

Query: 66  FQVINHGVSKRVLEETTRVFKEFHAMSGAEKAK--------ECSRDPNKSCRVYTSSENY 125
           FQVINHG  + ++ ET  V KEF A+   EK K        +      +  ++Y   E  
Sbjct: 64  FQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQL 123

Query: 126 MKEQTHYWRDALVFNCHPLDK-YVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGL 185
             E+  YW+D L   CHPLD+  V+ WP+ P KYREVV  Y V +RKL + +L+ I EGL
Sbjct: 124 SNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGL 183

Query: 186 KLEREYLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQ--VLKD 245
            L+  Y   E+S+   +L N+YPPCP+PS TLG   H D +LIT+L Q++ GLQ  ++KD
Sbjct: 184 GLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKD 243

Query: 246 GQWIGVHPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIME 305
             WI V PI  AFVVN+G  L+VI+N   +   HR VT+    R SIA L+ P     +E
Sbjct: 244 ATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIE 303

Query: 306 PAKCLINEANPPHYRSLMFKDFQTNYVPRAAD 327
           PAK L+N+ NPP Y+   + +F   Y+   +D
Sbjct: 304 PAKELLNQDNPPLYKPYSYSEFADIYLSDKSD 332

BLAST of Tan0014327 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 237.3 bits (604), Expect = 2.6e-61
Identity = 131/312 (41.99%), Postives = 189/312 (60.58%), Query Frame = 0

Query: 15  SVPESYVYPPEKRPGNVVVPMEKAI---PVVDLSADDRALLVRKILDASKEFGFFQVINH 74
           S+PES+V+  +K P     P  KA+   P++DLS   R  + R IL+A KE GFFQV+NH
Sbjct: 13  SLPESFVFASDKVP-----PATKAVVSLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNH 72

Query: 75  GVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKEQTHYWRDALVF 134
           GVSK+V+ +   + ++F  +  A+KA   S + +K  R++ S   Y      YWRD L  
Sbjct: 73  GVSKQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLF-SGATYDTGGEKYWRDCLRL 132

Query: 135 NC-HPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLEREYLGGEMS-E 194
            C  P+D  ++ WP  P   R+V+  +    R +  E+L L+ EG+ ++ +Y  G++S  
Sbjct: 133 ACPFPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGG 192

Query: 195 NPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPIDNAFV 254
           N  L +NHYP CPNP   LG   HCD +LIT+L    VNGL+V   G WI V P  NAFV
Sbjct: 193 NVILNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFV 252

Query: 255 VNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLINEANPPHY 314
           VN G  L+V++NG+LK++EHRA+TNS  +R S+A  + P  E ++ PAK  +++ NPP Y
Sbjct: 253 VNFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCY 312

Query: 315 RSLMFKDFQTNY 321
           R+ MF+DF   Y
Sbjct: 313 RTTMFRDFMRIY 317

BLAST of Tan0014327 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 7.1e-59
Identity = 127/325 (39.08%), Postives = 190/325 (58.46%), Query Frame = 0

Query: 13  VDSVPESYVYPPEKRPG-NVVVPMEKAIPVV---DLSADDRALLVRKILDASKEFGFFQV 72
           VD VP +YV P   RP  + V     +IP++   DL   +RA ++ +   A    GFFQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 73  INHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKEQTHYWRDA 132
            NHGV +  +++     +EF   S +E+ K  S D  K+ R+ TS  N  KE+   WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 133 LVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLEREYLGGEMS 192
           L  +C+P++ +++ WP  P  +REV   Y  ++R LVL +LE ISE L L ++ +   + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 193 ENPK-LLVNHYPPCPNPSLTLGLNQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPIDNA 252
           ++ + + +N+YP CP P LT GL  H D +LIT+L Q EV+GLQV KDG+WI V+P+ N 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 253 FVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLINEA--N 312
           F+VN+G  +QVISN   K+V HRAV NS   R SI     P ++ ++ PA+ LINE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 313 PPHYRSLMFKDFQTNYVPRAADTNA 330
           P  YR+  + ++   +   A DT +
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTES 338

BLAST of Tan0014327 vs. NCBI nr
Match: XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 608.2 bits (1567), Expect = 4.3e-170
Identity = 291/334 (87.13%), Postives = 306/334 (91.62%), Query Frame = 0

Query: 1   MGDVKFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDAS 60
           MG+VKFVSNWE V SVP  YVYPPEKRPGNVVVPM   IPV+DLS  DR LLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGVSKRVLEETTR+FK+FHAMSGAEKAKECSRDPNKSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLER 180
           QTH WRDAL+FNCHPLDKYVHFWPQNPPKYREVVGAYCV MR LVLEILEL+SEGL L R
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180

Query: 181 EYLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
            YLGGEMSENP +LVNHYPPCPNPSLTLGL+QHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNG LKA EHRAVTN+K SRQS+ YLVYPKDEV MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPHYRSLMFKDFQTNYVPRAADTNAVMQYI 335
           NEANPP YR+L FKDFQ NY+PRA+DT AVM+ I
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECI 334

BLAST of Tan0014327 vs. NCBI nr
Match: KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607.1 bits (1564), Expect = 9.5e-170
Identity = 289/334 (86.53%), Postives = 306/334 (91.62%), Query Frame = 0

Query: 1   MGDVKFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDAS 60
           MG+VKFVSNWE V SVP  YVYPPEKRPGNVVVPM  AIPV+DLS  DR LLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGVS+RVLEETTR+FK+FHAMSGAEK KECSRDPNKSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLER 180
           QTH WRDAL+FNCHPLDKYVHFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGL L R
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 EYLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
            YLGGEMSENP +LVNHYPPCPNPSLTLGL+QHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNG LKA EHR VTN+K SRQS+ YLVYPKDEV MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPHYRSLMFKDFQTNYVPRAADTNAVMQYI 335
           NEANPP YR+L FKDFQ NY+PRA+DT AVM+ I
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECI 334

BLAST of Tan0014327 vs. NCBI nr
Match: KAG7025486.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 605.9 bits (1561), Expect = 2.1e-169
Identity = 289/334 (86.53%), Postives = 306/334 (91.62%), Query Frame = 0

Query: 1   MGDVKFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDAS 60
           MG+VKFVSNWE V SVP  YVYPPEKRPGNVVVPM  AIPV+DLS  DR LLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGV +RVLEETTR+FK+FHAMSGAEKAKECSRDP KSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKE 120

Query: 121 QTHYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLER 180
           QTH WRDAL+FNCHPLDKYVHFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGL L R
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 EYLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
            YLGGEMSENP +LVNHYPPCPNPSLTLGL+QHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNG LKA EHRAVTN+K SRQS+ YLVYPKDEV MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPHYRSLMFKDFQTNYVPRAADTNAVMQYI 335
           NEANPP YR+L FKDFQ NY+PRA+DT AVM+ I
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECI 334

BLAST of Tan0014327 vs. NCBI nr
Match: XP_022959666.1 (hyoscyamine 6-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 604.4 bits (1557), Expect = 6.1e-169
Identity = 288/334 (86.23%), Postives = 305/334 (91.32%), Query Frame = 0

Query: 1   MGDVKFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDAS 60
           MG+VKFVSNWE V SVP  YVYPPEKRPGNVVVPM  AIPV+DLS  DR LLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGVS+RVLEETTR+FK+FHAMSGAEK KECSRDPNKSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLER 180
           QTH WRDAL+FNCHPLDKYVHFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGL L R
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 EYLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
            YLGGEMSENP +LVNHYP CPNPSLTLGL+QHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNG LKA EHR VTN+K SRQS+ YLVYPKDEV MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPHYRSLMFKDFQTNYVPRAADTNAVMQYI 335
           NEANPP YR+L FKDFQ NY+PRA+DT AVM+ I
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECI 334

BLAST of Tan0014327 vs. NCBI nr
Match: XP_023004374.1 (hyoscyamine 6-dioxygenase-like [Cucurbita maxima])

HSP 1 Score: 602.1 bits (1551), Expect = 3.1e-168
Identity = 288/334 (86.23%), Postives = 305/334 (91.32%), Query Frame = 0

Query: 1   MGDVKFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDAS 60
           MG+VKFVSNWE V SVP  Y+YPPEKRPGNVVVPM  AIPVVDLS  DR LLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGVS+RVLEETTR+FK+FHAMSGAEKAKECSRDP KSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120

Query: 121 QTHYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLER 180
           QTH WRDAL+FNCHPL+KYVHFWPQNPPKYREVVGAYCVAMR LVLEILEL+SEGL L R
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180

Query: 181 EYLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
            YL  EMSENP +LVNHYPPCPNPSLTLGL+QHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNG LKA EHRAVTN+K SRQS+ YLVYPKDEV MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPHYRSLMFKDFQTNYVPRAADTNAVMQYI 335
           NEANPP YR+L FKDFQ NY+PRA+DT AVM+ I
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECI 334

BLAST of Tan0014327 vs. ExPASy TrEMBL
Match: A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.0e-169
Identity = 288/334 (86.23%), Postives = 305/334 (91.32%), Query Frame = 0

Query: 1   MGDVKFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDAS 60
           MG+VKFVSNWE V SVP  YVYPPEKRPGNVVVPM  AIPV+DLS  DR LLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGVS+RVLEETTR+FK+FHAMSGAEK KECSRDPNKSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLER 180
           QTH WRDAL+FNCHPLDKYVHFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGL L R
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 EYLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
            YLGGEMSENP +LVNHYP CPNPSLTLGL+QHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNG LKA EHR VTN+K SRQS+ YLVYPKDEV MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPHYRSLMFKDFQTNYVPRAADTNAVMQYI 335
           NEANPP YR+L FKDFQ NY+PRA+DT AVM+ I
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECI 334

BLAST of Tan0014327 vs. ExPASy TrEMBL
Match: A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.5e-168
Identity = 288/334 (86.23%), Postives = 305/334 (91.32%), Query Frame = 0

Query: 1   MGDVKFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDAS 60
           MG+VKFVSNWE V SVP  Y+YPPEKRPGNVVVPM  AIPVVDLS  DR LLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGVS+RVLEETTR+FK+FHAMSGAEKAKECSRDP KSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120

Query: 121 QTHYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLER 180
           QTH WRDAL+FNCHPL+KYVHFWPQNPPKYREVVGAYCVAMR LVLEILEL+SEGL L R
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180

Query: 181 EYLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
            YL  EMSENP +LVNHYPPCPNPSLTLGL+QHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNG LKA EHRAVTN+K SRQS+ YLVYPKDEV MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPHYRSLMFKDFQTNYVPRAADTNAVMQYI 335
           NEANPP YR+L FKDFQ NY+PRA+DT AVM+ I
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECI 334

BLAST of Tan0014327 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.3e-168
Identity = 282/337 (83.68%), Postives = 305/337 (90.50%), Query Frame = 0

Query: 2   GDVKFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDASK 61
           G  KFVSNWE V+SVPESYVYPPEKRPGN+VVPM KAIPV+DLS  DR LLVRKILDASK
Sbjct: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63

Query: 62  EFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKEQ 121
           EFGFFQ+INHGVSK+V EET R+FKEFHAMSG EKAKECS+DPN+SCRVYTSSENY KEQ
Sbjct: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123

Query: 122 THYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLERE 181
            H WRDAL+FNCHPL+KYVHFWPQ PPKYREVVGAYC+AMRKLVLEILEL+SEGL L + 
Sbjct: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
           Y GGEMSENP LLVNHYPPCPNPSLTLGL+QHCDPSLITILFQ+VNGLQVLKDGQWIGV 
Sbjct: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLIN 301
           PIDNAFVVNIGFVLQ+I+NG L+A EHRAVTNSKTSRQS+ YLVYPKDE +MEPAKC+IN
Sbjct: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303

Query: 302 EANPPHYRSLMFKDFQTNYVPRAADTNAVMQYIRVEE 339
           EANPP YRSL FKDFQ NY+PRA DT AVMQYI   +
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340

BLAST of Tan0014327 vs. ExPASy TrEMBL
Match: A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.8e-167
Identity = 284/337 (84.27%), Postives = 303/337 (89.91%), Query Frame = 0

Query: 2   GDVKFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDASK 61
           GD KFVSNWE  +SVPESYVYPPEKRPGNVVVPM KAIPV+DLS  DRALLVRKIL ASK
Sbjct: 4   GDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASK 63

Query: 62  EFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKEQ 121
           E GFFQ+INHGVSKRV E T R+FKEFHAMSG EKAKECS+DPN+SCRVYTSSENY KEQ
Sbjct: 64  ESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQ 123

Query: 122 THYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLERE 181
            H WRDAL+FNCHPL+KYVHFWPQNPPKYREVVGAYCVAMRKLVLEILEL+SEGL L + 
Sbjct: 124 IHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
           YLGGEMSENP LLVNHYPPCPNPSLTLGL+QHCDPSLITILFQ+VNGLQVLKDGQWIGV 
Sbjct: 184 YLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLIN 301
           PID AFVVNIGFVLQ+I+NG L+A EHRAVTNSKTSRQS+ YLVYPKDE  MEPAKC+IN
Sbjct: 244 PIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMIN 303

Query: 302 EANPPHYRSLMFKDFQTNYVPRAADTNAVMQYIRVEE 339
           EANPP YRSL FKDFQ NY+PRA DT AVMQYI   +
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340

BLAST of Tan0014327 vs. ExPASy TrEMBL
Match: A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.4e-158
Identity = 270/335 (80.60%), Postives = 297/335 (88.66%), Query Frame = 0

Query: 1   MGD-VKFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDA 60
           MGD V FVSNW  V+SVPESYVYPPEKRPGNV VP+E AIPVVDL+  DRA LV KILDA
Sbjct: 1   MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA 60

Query: 61  SKEFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMK 120
           S+EFGFFQVINHGVSK ++EET RVF+EFHAMSG EK +ECS+DPN+SCRVYTSSEN++K
Sbjct: 61  SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK 120

Query: 121 EQTHYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLE 180
           E+TH WRDAL+FNCHPLDKYVHFWPQNP  YREVVGAYC+ MRKLV+EILELISEGL L 
Sbjct: 121 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 180

Query: 181 REYLGGEMSENPKLLVNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLKDGQWIG 240
           + Y G EMSENP LLVNHYPPCPNPSLTLGL+QHCDPSLITILFQ++NGLQVLKDGQWIG
Sbjct: 181 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 240

Query: 241 VHPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCL 300
           VHP+DNAFVVNIGFVLQV+SNG LKA EHRAV N+K SRQSI YLVYPKD+  MEPAKC+
Sbjct: 241 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 300

Query: 301 INEANPPHYRSLMFKDFQTNYVPRAADTNAVMQYI 335
           INEANP  Y SL FKDFQ NY+P AADT AV+QYI
Sbjct: 301 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYI 335

BLAST of Tan0014327 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.2e-71
Identity = 142/328 (43.29%), Postives = 205/328 (62.50%), Query Frame = 0

Query: 5   KFVSNWEAVDSVPESYVYPPEKRPGNVVVPMEKAIPVVDLSADDRALLVRKILDASKEFG 64
           K +S      ++PE+YV P   RP    V   +  P++DLS+ DR+ L+++I  A   FG
Sbjct: 4   KLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFG 63

Query: 65  FFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKEQTHY 124
           FFQVINHGV+K++++E   V +EF +MS  EK K  S DP K+ R+ TS  N  KE+ + 
Sbjct: 64  FFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNN 123

Query: 125 WRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLEREYLG 184
           WRD L  +C+P+ KYV+ WP NPP ++E+V  Y   +R++  +I ELISE L LE++Y+ 
Sbjct: 124 WRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMK 183

Query: 185 GEMSENPK-LLVNHYPPCPNPSLTLGLNQHCDPSLITILFQE--VNGLQVLKDGQWIGVH 244
             + E  + + VN+YPPCP P LT GL  H DP+ +TIL Q+  V GLQ+L DGQW  V+
Sbjct: 184 KVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVN 243

Query: 245 PIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCL-- 304
           P  +AFV+NIG  LQ +SNG+ K+V HRAVTN++  R S+A  + P D  +M PAK L  
Sbjct: 244 PHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWE 303

Query: 305 -INEANPPHYRSLMFKDFQTNYVPRAAD 327
             ++   P Y+   + ++   +  R  D
Sbjct: 304 AEDDETKPVYKDFTYAEYYKKFWSRNLD 330

BLAST of Tan0014327 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 229.2 bits (583), Expect = 5.0e-60
Identity = 127/325 (39.08%), Postives = 190/325 (58.46%), Query Frame = 0

Query: 13  VDSVPESYVYPPEKRPG-NVVVPMEKAIPVV---DLSADDRALLVRKILDASKEFGFFQV 72
           VD VP +YV P   RP  + V     +IP++   DL   +RA ++ +   A    GFFQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 73  INHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKEQTHYWRDA 132
            NHGV +  +++     +EF   S +E+ K  S D  K+ R+ TS  N  KE+   WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 133 LVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLEREYLGGEMS 192
           L  +C+P++ +++ WP  P  +REV   Y  ++R LVL +LE ISE L L ++ +   + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 193 ENPK-LLVNHYPPCPNPSLTLGLNQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPIDNA 252
           ++ + + +N+YP CP P LT GL  H D +LIT+L Q EV+GLQV KDG+WI V+P+ N 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 253 FVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLINEA--N 312
           F+VN+G  +QVISN   K+V HRAV NS   R SI     P ++ ++ PA+ LINE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 313 PPHYRSLMFKDFQTNYVPRAADTNA 330
           P  YR+  + ++   +   A DT +
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTES 338

BLAST of Tan0014327 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 225.3 bits (573), Expect = 7.2e-59
Identity = 124/328 (37.80%), Postives = 192/328 (58.54%), Query Frame = 0

Query: 7   VSNWEAVDSVPESYVYPPEKRPG-NVVVPMEKAIPVV---DLSADDRALLVRKILDASKE 66
           VS++ +   +P +YV P   RP  + V     +IP++   DL   +RA++V+++  A   
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 67  FGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKEQT 126
           +GFFQ+ NHGV    + +   V +EF     +E+ K  S DP K+ R+ TS  N   ++ 
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130

Query: 127 HYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLEREY 186
             WRD L  +C P++ ++  WP +P  +REV   Y  ++R LVL +LE ISE L LE ++
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190

Query: 187 LGGEMSENPK-LLVNHYPPCPNPSLTLGLNQHCDPSLITILFQ-EVNGLQVLKDGQWIGV 246
           +   + ++ + +  N+YPPCP P LT GL  H DP++IT+L Q +V+GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250

Query: 247 HPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLI 306
            PI N F+VNIG  +QVISN   K+V HRAV N++  R SI    +P  + ++ PA  L+
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310

Query: 307 NEANP-PHYRSLMFKDFQTNYVPRAADT 328
           NE +    YR+  F ++   +  R+  T
Sbjct: 311 NEQDSLAIYRTYPFVEYWDKFWNRSLAT 337

BLAST of Tan0014327 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 209.9 bits (533), Expect = 3.1e-54
Identity = 122/317 (38.49%), Postives = 175/317 (55.21%), Query Frame = 0

Query: 13  VDSVPESYVYPPEKRP--GNVVVPMEKAIPVVDLSADD----RALLVRKILDASKEFGFF 72
           V  VP+ YV PP +RP  G+ +   E  +PV+DLS       R+L + +I  A KEFGFF
Sbjct: 24  VPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGFF 83

Query: 73  QVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSENYMKEQTHYWR 132
           QVINHG+   V+ +      +F  +   EK    S + ++  R Y +S N+  ++ HYWR
Sbjct: 84  QVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYWR 143

Query: 133 DALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEGLKLEREYLGGE 192
           D +    HPL K++  WP NPP Y++ VG Y  A   L  +++E ISE L LE+ YL  E
Sbjct: 144 DFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQEE 203

Query: 193 MSENPKLL-VNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLK-DGQWIGVHPID 252
           + E  +++ VN YP CP P + LG+  H D S +TIL Q   GLQ++  +  W+ V  I+
Sbjct: 204 IEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYIE 263

Query: 253 NAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIMEPAKCLINEAN 312
            A +V +G  ++V+SNG+ K+V HR   N +  R S A L        + PA  L+N  N
Sbjct: 264 GALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPNN 323

Query: 313 PPHYRSLMFKDFQTNYV 322
            P Y    F DF  NY+
Sbjct: 324 APAYGEFSFNDF-LNYI 338

BLAST of Tan0014327 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 196.1 bits (497), Expect = 4.7e-50
Identity = 122/330 (36.97%), Postives = 172/330 (52.12%), Query Frame = 0

Query: 6   FVSNWEAVDS----VPESYVYPPEKRP--GNVVVPMEKAIPVVDLSADDRALL----VRK 65
           F S  E  +S    VP  Y+ PP +RP  G  +      +PV+DLS     LL    + +
Sbjct: 13  FTSAMELTESGDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFLHDPLLRLCVIHE 72

Query: 66  ILDASKEFGFFQVINHGVSKRVLEETTRVFKEFHAMSGAEKAKECSRDPNKSCRVYTSSE 125
           I  A K FGFFQVINHG+S  V+++       F  +   EK    S +  +  R Y +S 
Sbjct: 73  IELACKGFGFFQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVR-YGTSI 132

Query: 126 NYMKEQTHYWRDALVFNCHPLDKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELISEG 185
           N+  ++ HYWRD +    HPL  +++ WP NPP Y+E VG Y  A   L  +++E ISE 
Sbjct: 133 NHSTDRVHYWRDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISES 192

Query: 186 LKLEREYLGGEMSENPKLL-VNHYPPCPNPSLTLGLNQHCDPSLITILFQEVNGLQVLK- 245
           L LE+ YL  E+ E  +++ VN YP CP P + LG+  H D   +TIL Q   GLQ+   
Sbjct: 193 LGLEKNYLQEEIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKDC 252

Query: 246 DGQWIGVHPIDNAFVVNIGFVLQVISNGMLKAVEHRAVTNSKTSRQSIAYLVYPKDEVIM 305
           +  W+ V  I+ A +V +G  ++V+SNG+ K+V HR   N    R S A L        +
Sbjct: 253 NNNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHKKI 312

Query: 306 EPAKCLINEANPPHYRSLMFKDFQTNYVPR 324
            PA  L+NE  P  Y    F DF  +Y+ R
Sbjct: 313 SPATQLVNENKPAAYGEFSFNDF-LDYISR 340