Tan0013947 (gene) Snake gourd v1

Overview


Name	Tan0013947
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	(1->3)-beta-glucan endohydrolase
Location	LG09: 63182510 .. 63183523 (+)
RNA-Seq Expression	Tan0013947
Synteny	Tan0013947

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAGCTTTCCTACATGATTATCATCTTCATGTCTGCAATGACATCCGTCGTTGGAACATACGACATTCTTCTTGGTGCCTGCTATGGTTTGGCTGGTAACAACCTGCCACAGCCATCAGACGTCGTGCGCCTTTGTCTAAAACACAAAATTCGTCGTGTTCGATTGTACGAACCGAACCTCGATGTTTTGAAAGAGTTTCGCGGTGTAGGAATCGACCTCTCTTTCAGCATACCCAACTCTATGTTAACGGACATGGCCACCAACAACTCTATCGTGGAAGAGTGGTTTAACACCTCACGCCGAACCCTTCATCGGCGATTTCGCCATCAATTACATCATCATCGTCGCGGCGACAAGGCCATCCCGGGCCTCCACGACTTCATATTGCCAGTCATGAAGTCCCTTCAAGATCTCCTCAACGCTCATTATCTTGGACAAGTGAAACTCACAACATTAGTTGGCTATAGAGCCATATTGGCTGTTAAAAACCCTCCCTCTAGCGGCACATTCAACCCAATTATTATGCGACAACATGAGAGAAATCCTTAAGTTTTTGTCGAGAGAAGGTCGCCCTTGATGGTTAGTGTAAATCCATATCAAGCCTATGTTTACGATGGGAACATAAGTTTGAGTTACGCGACATTTACCGAAAAAAATACGGTGGTATACGATGGGACGTTAAGTTATAATAACTTGTTCGATGAAATGGTGGATGCATTTTATGCGGCCATCGATAAGGAAAATGTCGGAGAAGTGGCCATCGTCATTGGAGAAACTGGATGGCCGACTTGTGGGAATTATGGCGTTGCAACACCGTCGCTTGCAGCGACGTATAACGAGAATTTTAAGAGCCATATTTCGTCTGGAAAGGGGACACCAAAGAAGCCAAATGTTTATATGGAAGGATTCATTGAGAGTATGTTCAATGAGAATGAAAAACCTGAAGGGGAGTCACGATGTTATGGCATCTTCAATGTTGACTCCACACCTATTTATCCTTTGTTTTAA

mRNA sequence

ATGGCAAAGCTTTCCTACATGATTATCATCTTCATGTCTGCAATGACATCCGTCGTTGGAACATACGACATTCTTCTTGGTGCCTGCTATGGTTTGGCTGGTAACAACCTGCCACAGCCATCAGACGTCGTGCGCCTTTGTCTAAAACACAAAATTCGTCGTGTTCGATTGTACGAACCGAACCTCGATGTTTTGAAAGAGTTTCGCGGTGTAGGAATCGACCTCTCTTTCAGCATACCCAACTCTATGTTAACGGACATGGCCACCAACAACTCTATCGTGGAAGAGTGGTTTAACACCTCACGCCGAACCCTTCATCGGCGATTTCGCCATCAATTACATCATCATCGTCGCGGCGACAAGGCCATCCCGGGCCTCCACGACTTCATATTGCCAGTCATGAAGTCCCTTCAAGATCTCCTCAACGCTCATTATCTTGGACAAAGAAGGTCGCCCTTGATGGTTAGTGTAAATCCATATCAAGCCTATGTTTACGATGGGAACATAAGTTTGAGTTACGCGACATTTACCGAAAAAAATACGGTGGTATACGATGGGACGTTAAGTTATAATAACTTGTTCGATGAAATGGTGGATGCATTTTATGCGGCCATCGATAAGGAAAATGTCGGAGAAGTGGCCATCGTCATTGGAGAAACTGGATGGCCGACTTGTGGGAATTATGGCGTTGCAACACCGTCGCTTGCAGCGACGTATAACGAGAATTTTAAGAGCCATATTTCGTCTGGAAAGGGGACACCAAAGAAGCCAAATGTTTATATGGAAGGATTCATTGAGAGTATGTTCAATGAGAATGAAAAACCTGAAGGGGAGTCACGATGTTATGGCATCTTCAATGTTGACTCCACACCTATTTATCCTTTGTTTTAA

Coding sequence (CDS)

ATGGCAAAGCTTTCCTACATGATTATCATCTTCATGTCTGCAATGACATCCGTCGTTGGAACATACGACATTCTTCTTGGTGCCTGCTATGGTTTGGCTGGTAACAACCTGCCACAGCCATCAGACGTCGTGCGCCTTTGTCTAAAACACAAAATTCGTCGTGTTCGATTGTACGAACCGAACCTCGATGTTTTGAAAGAGTTTCGCGGTGTAGGAATCGACCTCTCTTTCAGCATACCCAACTCTATGTTAACGGACATGGCCACCAACAACTCTATCGTGGAAGAGTGGTTTAACACCTCACGCCGAACCCTTCATCGGCGATTTCGCCATCAATTACATCATCATCGTCGCGGCGACAAGGCCATCCCGGGCCTCCACGACTTCATATTGCCAGTCATGAAGTCCCTTCAAGATCTCCTCAACGCTCATTATCTTGGACAAAGAAGGTCGCCCTTGATGGTTAGTGTAAATCCATATCAAGCCTATGTTTACGATGGGAACATAAGTTTGAGTTACGCGACATTTACCGAAAAAAATACGGTGGTATACGATGGGACGTTAAGTTATAATAACTTGTTCGATGAAATGGTGGATGCATTTTATGCGGCCATCGATAAGGAAAATGTCGGAGAAGTGGCCATCGTCATTGGAGAAACTGGATGGCCGACTTGTGGGAATTATGGCGTTGCAACACCGTCGCTTGCAGCGACGTATAACGAGAATTTTAAGAGCCATATTTCGTCTGGAAAGGGGACACCAAAGAAGCCAAATGTTTATATGGAAGGATTCATTGAGAGTATGTTCAATGAGAATGAAAAACCTGAAGGGGAGTCACGATGTTATGGCATCTTCAATGTTGACTCCACACCTATTTATCCTTTGTTTTAA

Protein sequence

MAKLSYMIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLKEFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIPGLHDFILPVMKSLQDLLNAHYLGQRRSPLMVSVNPYQAYVYDGNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDAFYAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVYMEGFIESMFNENEKPEGESRCYGIFNVDSTPIYPLF

Homology

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BLAST of Tan0013947 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 8.9e-50
Identity = 118/332 (35.54%), Postives = 181/332 (54.52%), Query Frame = 0


Query: 8   IIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLKE 67
           I++++++  S   T    +G  YGL G+NLP PS+V+ L     I R+R+++PN +VL  
Sbjct: 16  IVLYVNSNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNA 75

Query: 68  FRG-VGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIPG- 127
            RG   I+++  + +  L  +A +   V+ WF  +  +        +     G++ IPG 
Sbjct: 76  LRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADV--NITFITVGNEVIPGP 135

Query: 128 LHDFILPVMKSLQDLLNAH---------------------------------------YL 187
           +   +LPVM+SL +L+ +                                         L
Sbjct: 136 IGPQVLPVMQSLTNLVKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLL 195

Query: 188 GQRRSPLMVSVNPYQAYVYD-GNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDAFYAAI 247
            Q  +P++V++ PY AY  D  NI L YA+F  K+ VV DG+L Y+N+FD + DAF  A+
Sbjct: 196 SQTSTPILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAM 255

Query: 248 DKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVYMEGFI 297
           +KE V  + +V+ ETGWP+ GN    TP++A+TYN NF  HI+SGKGTPK+PN  M GF+
Sbjct: 256 EKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFL 315

[Close]

BLAST of Tan0013947 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.1e-47
Identity = 112/314 (35.67%), Postives = 169/314 (53.82%), Query Frame = 0


Query: 26  LGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLKEFRG-VGIDLSFSIPNSML 85
           +G  YGL G+NLP PS V+ L     I ++R+++PN +VL   RG   I ++  + +  L
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 86  TDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIPG-LHDFILPVMKSLQDLLNA 145
             ++ +   V+ WF T+           +     G++ IPG +   +LPVM+SL +L+ +
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDI--NIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKS 162

Query: 146 H---------------------------------------YLGQRRSPLMVSVNPYQAYV 205
                                                    L Q  SP++V + PY +Y 
Sbjct: 163 RNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYA 222

Query: 206 YD-GNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDAFYAAIDKENVGEVAIVIGETGWP 265
            D  +I L YATF  +  VV DG+L Y+N+FD + DAF  A++KE V ++ +V+ ETGWP
Sbjct: 223 SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 282

Query: 266 TCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVYMEGFIESMFNENEKPEGESRCYG 297
           + GN  + TP +A TYN NF  HI+SGKGTPK+PN  ++GF+ + FNEN+KP G  + +G
Sbjct: 283 SAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFG 342

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BLAST of Tan0013947 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 176.4 bits (446), Expect = 4.8e-43
Identity = 111/338 (32.84%), Postives = 167/338 (49.41%), Query Frame = 0


Query: 2   AKLSYMIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPN 61
           A LS      + ++   V T    +G CYG+ GNNLP PS+VV L   + I R+RLY+PN
Sbjct: 5   ASLSIKGFALLVSVLVAVPTRVQSIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPN 64

Query: 62  LDVLKEFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDK 121
              L+  R   I +   +P S +  +A+N S   +W    R  +         +   G++
Sbjct: 65  QAALQALRNSNIQVLLDVPRSDVQSLASNPSAAGDWIR--RNVVAYWPSVSFRYIAVGNE 124

Query: 122 AIPG--LHDFILPVMKSLQDLLNA------------------------------------ 181
            IPG  L  +ILP M+++ + L++                                    
Sbjct: 125 LIPGSDLAQYILPAMRNIYNALSSAGLQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAY 184

Query: 182 -----HYLGQRRSPLMVSVNPYQAYVYD-GNISLSYATFTEKNTVVYDGTLSYNNLFDEM 241
                 +L    +PL+V+V PY +Y  + G ISL YA FT    VV DG  SY NLFD +
Sbjct: 185 LSPIVQFLASNGAPLLVNVYPYFSYTGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAI 244

Query: 242 VDAFYAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKP 296
           VDA +AA+++     VA+V+ E+GWP+ G    A+ S A TYN+N   H+  G GTP++P
Sbjct: 245 VDAVFAALERVGGANVAVVVSESGWPSAGGGAEASTSNARTYNQNLIRHV--GGGTPRRP 304

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BLAST of Tan0013947 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 171.4 bits (433), Expect = 1.5e-41
Identity = 108/338 (31.95%), Postives = 172/338 (50.89%), Query Frame = 0


Query: 7   MIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLK 66
           +++  + + T +VG   +  G CYG+ GNNLP  S VV+L     IRR+RLY+PN   L+
Sbjct: 16  ILLGLLVSSTEIVGAQSV--GVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQ 75

Query: 67  EFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIP-- 126
             RG  I++   +PNS L ++A N S    W   + R      +    +   G++  P  
Sbjct: 76  ALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVK--FRYIAVGNEVSPVT 135

Query: 127 ---GLHDFILPVMKSLQDLLNA-------------------------------------- 186
               L  ++LP M+++++ +++                                      
Sbjct: 136 GTSSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFID 195

Query: 187 ---HYLGQRRSPLMVSVNPYQAYVYDGN---ISLSYATFTEKNTVVYDGTLSYNNLFDEM 246
               ++ +  SPL+V++ PY  + Y GN   ISL YA FT  N VV DG+L Y NLFD M
Sbjct: 196 PIIGFVRRINSPLLVNIYPY--FSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAM 255

Query: 247 VDAFYAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKP 296
            DA YAA+ +   G + IV+ E+GWP+ G +  AT + AATY +N   H+   +G+P++P
Sbjct: 256 SDAVYAALSRAGGGSIEIVVSESGWPSAGAF-AATTNNAATYYKNLIQHVK--RGSPRRP 315

[Close]

BLAST of Tan0013947 vs. ExPASy Swiss-Prot
Match: P52407 (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis OX=3981 GN=HGN1 PE=1 SV=2)

HSP 1 Score: 159.8 bits (403), Expect = 4.6e-38
Identity = 103/330 (31.21%), Postives = 166/330 (50.30%), Query Frame = 0


Query: 2   AKLSYMIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPN 61
           ++ + M+++F  A +  VG  D  +G CYG+ GNNLP  S+V+ L  K  I R+R+Y+PN
Sbjct: 16  SRTTVMLLLFFFAAS--VGITDAQVGVCYGMQGNNLPPVSEVIALYKKSNITRMRIYDPN 75

Query: 62  LDVLKEFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRR----TLHRRFRHQLHHHR 121
             VL+  RG  I+L   +PNS L  + TN S  + W   + R    ++  R+    +   
Sbjct: 76  RAVLEALRGSNIELILGVPNSDLQSL-TNPSNAKSWVQKNVRGFWSSVLFRYIAVGNEIS 135

Query: 122 RGDKAIPGLHDFILPVMKSLQDLLNA---------------------------------- 181
             ++    L  F+LP M+++ D + +                                  
Sbjct: 136 PVNRGTAWLAQFVLPAMRNIHDAIRSAGLQDQIKVSTAIDLTLVGNSYPPSAGAFRDDVR 195

Query: 182 -------HYLGQRRSPLMVSVNPYQAYVYD-GNISLSYATFTEKNTVVYDGTLSYNNLFD 241
                   +L   RSPL+ ++ PY  Y Y+  +ISL YA FT  + VV+DG   Y NLFD
Sbjct: 196 SYLDPIIGFLSSIRSPLLANIYPYFTYAYNPRDISLPYALFTSPSVVVWDGQRGYKNLFD 255

Query: 242 EMVDAFYAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPK 286
             +DA Y+A+++ + G + +V+ E+GWP+ G +  AT     TY  N   H+    GTPK
Sbjct: 256 ATLDALYSALERASGGSLEVVVSESGWPSAGAF-AATFDNGRTYLSNLIQHVKG--GTPK 315

[Close]

BLAST of Tan0013947 vs. NCBI nr
Match: KAG6590489.1 (putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 380.2 bits (975), Expect = 1.7e-101
Identity = 200/331 (60.42%), Postives = 233/331 (70.39%), Query Frame = 0


Query: 7   MIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLK 66
           MI +FM+ M+ VVG YD+LLGA YGL G+NLP PS VV+LC K+ I RVRL  PNLDV++
Sbjct: 1   MIGLFMADMSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIE 60

Query: 67  EFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIPGL 126
            FRG  IDLSF +PN ML +MATN ++VEEWFNT            +++   GDKAIPGL
Sbjct: 61  GFRGTEIDLSFGVPNDMLPNMATNKTLVEEWFNTYVEPYTNDC--TINYIVVGDKAIPGL 120

Query: 127 HDFILPVMKSLQDLLNAHYLGQRR------------------------------------ 186
            D ILPVMKSLQDLLNAHY G+ +                                    
Sbjct: 121 DDNILPVMKSLQDLLNAHYFGEVKLTTLVGFNALAVTNPPSSGAFNPAIVENMRGILEFL 180

Query: 187 ----SPLMVSVNPYQAYVYDGNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDAFYAAID 246
               SPLMVSV  Y  YVY GNISL YAT +E+NT+V DG LSYNNLFDE+VDAFYAA+D
Sbjct: 181 RGEGSPLMVSVYTYDQYVYGGNISLGYATLSEQNTLVRDGMLSYNNLFDEIVDAFYAAMD 240

Query: 247 KENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVYMEGFIE 297
           KENVG+VAIV GETGWP+CGNY   TP LAA YN NFK+HISSGKGTP+K N+Y+EGFI 
Sbjct: 241 KENVGDVAIVFGETGWPSCGNYNATTPLLAAEYNRNFKNHISSGKGTPRKENIYIEGFIR 300

[Close]

BLAST of Tan0013947 vs. NCBI nr
Match: XP_022968751.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita maxima])

HSP 1 Score: 379.0 bits (972), Expect = 3.7e-101
Identity = 200/337 (59.35%), Postives = 235/337 (69.73%), Query Frame = 0


Query: 1   MAKLSYMIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEP 60
           M K+S+MI +FM+ M  +VG YD+LLGA YGL G+NL  P+ VV+LC K+ I RVRL   
Sbjct: 1   MVKISHMIGLFMADMFLIVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRLEVS 60

Query: 61  NLDVLKEFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGD 120
           N+DV++ FRG  IDLS  +PN ML +MATN ++VEEWFNT        +   +++   GD
Sbjct: 61  NIDVIEAFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDY--IINYIVVGD 120

Query: 121 KAIPGLHDFILPVMKSLQDLLNAHYLGQRR------------------------------ 180
           KAIPGL D ILPVMKSLQDLLNAHY G+ +                              
Sbjct: 121 KAIPGLDDNILPVMKSLQDLLNAHYFGEAKLTTLVGFNALAVTNPPSSGAFNPAIVENMR 180

Query: 181 ----------SPLMVSVNPYQAYVYDGNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDA 240
                     SPLMVSV  Y  YVY GN SL YAT +E+N++V DG LSYNNLFDEMVDA
Sbjct: 181 GILEFLRGEGSPLMVSVYTYDQYVYGGNRSLGYATLSEQNSLVRDGMLSYNNLFDEMVDA 240

Query: 241 FYAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVY 297
           FYAAIDKENVG+VAIVIGETGWPTCGNY   TP LAA YN NFKSHISSGKGTP+K N+Y
Sbjct: 241 FYAAIDKENVGDVAIVIGETGWPTCGNYNATTPLLAAEYNRNFKSHISSGKGTPRKENIY 300

[Close]

BLAST of Tan0013947 vs. NCBI nr
Match: XP_038880685.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida])

HSP 1 Score: 364.4 bits (934), Expect = 9.4e-97
Identity = 188/337 (55.79%), Postives = 240/337 (71.22%), Query Frame = 0


Query: 1   MAKLSYMIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEP 60
           MAKLS++I+IFM  ++S+VG YD+LLGA Y LAGNNLP P +VV+LC K+ IRRVRLYEP
Sbjct: 1   MAKLSHIIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEP 60

Query: 61  NLDVLKEFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGD 120
           NL VL+ FRG+GID+SF +PN+++ +MATN++ VEEWF          F   +++   GD
Sbjct: 61  NLTVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDF--TINYIVVGD 120

Query: 121 KAIPGLHDFILPVMKSLQDLLNA------------------------------------- 180
           K IPGL   IL VM+SLQ LLNA                                     
Sbjct: 121 KVIPGLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMR 180

Query: 181 ---HYLGQRRSPLMVSVNPYQAYVYDGNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDA 240
              H+L ++ SPLMVS+ P++ Y Y GN+SL YATF E+N  + DG LSYNNLFDEM+DA
Sbjct: 181 GILHFLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDA 240

Query: 241 FYAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVY 297
           FYAAIDKE+VG+VAI +GETGWPTCG+  + T S+AATYN NFK+HI+SGKGTP K N+Y
Sbjct: 241 FYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIY 300

[Close]

BLAST of Tan0013947 vs. NCBI nr
Match: XP_022136187.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia])

HSP 1 Score: 342.8 bits (878), Expect = 2.9e-90
Identity = 185/338 (54.73%), Postives = 225/338 (66.57%), Query Frame = 0


Query: 1   MAKLSYMIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEP 60
           MAKL+++I+ FM++M S V   D+LLG  YGL G+N+P P  VV+LC+K+ IRR+RL EP
Sbjct: 1   MAKLAHIIVFFMASM-SAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEP 60

Query: 61  NLDVLKEFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGD 120
           NL+V   F G GID+SF +PN ML +M  N S V EWFNT        F   +++   G+
Sbjct: 61  NLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDF--VINYIIVGN 120

Query: 121 KAIPGLHDFILPVMKSLQDLLNAHYLGQRR------------------------------ 180
           KAIPGL D ILPVMKSLQ+LLNA YLGQ +                              
Sbjct: 121 KAIPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNI 180

Query: 181 -----------SPLMVSVNPYQAYVYDGN-ISLSYATFTEKNTVVYDGTLSYNNLFDEMV 240
                      SPLMVSV PY  Y Y    ISL+YATF  K  VV+DG LSY+NLFD MV
Sbjct: 181 REILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMV 240

Query: 241 DAFYAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPN 297
           D FYAA+DK  VG+V + +GETGWPTCGN  +ATP  A+ YN+NFKSHISSG+GTPK+PN
Sbjct: 241 DPFYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPN 300

[Close]

BLAST of Tan0013947 vs. NCBI nr
Match: KAG6590491.1 (putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 342.4 bits (877), Expect = 3.8e-90
Identity = 187/326 (57.36%), Postives = 224/326 (68.71%), Query Frame = 0


Query: 12  MSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLKEFRGV 71
           M++M+SVVG Y +LLGA Y   G+NLP PS VV+L LK+ I RVRL  P+LD+L+ FRG+
Sbjct: 1   MASMSSVVGAYVVLLGAYY---GDNLPSPSLVVQLYLKYNISRVRLKVPSLDILEAFRGI 60

Query: 72  GIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIPGLHDFIL 131
            I +SF +PN ML +MATN ++VE WFNT    +     + +++   GDKAIPGL   IL
Sbjct: 61  EIGISFGVPNDMLPNMATNKTLVEVWFNT--YIVPYVDDYTINYIVVGDKAIPGLDGNIL 120

Query: 132 PVMKSLQDLLNAHY----------------------------------------LGQRRS 191
           PVMKSLQDLLNAHY                                        LG++ S
Sbjct: 121 PVMKSLQDLLNAHYLGRVKLTTLVGFKSLAVTNPPSSGAFYPTIVENMRGILKFLGRQGS 180

Query: 192 PLMVSVNPYQAYVYDGNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDAFYAAIDKENVG 251
           PLMVS+N Y  YVY GNISL YAT +E+N+ V+DG LSYNNLFDEMVDAFYAAIDK NV 
Sbjct: 181 PLMVSLNTYNRYVYGGNISLGYATLSEQNSTVHDGMLSYNNLFDEMVDAFYAAIDKANVR 240

Query: 252 EVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVYMEGFIESMFNE 297
           +V IV+GETGWPTCGN    TP LA+ YN NFKSHISSGKGTPKK N+Y+EGFI S+FNE
Sbjct: 241 DVGIVVGETGWPTCGNNNATTPLLASEYNRNFKSHISSGKGTPKKKNIYIEGFIRSIFNE 300

[Close]

BLAST of Tan0013947 vs. ExPASy TrEMBL
Match: A0A6J1HVR6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=3 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.8e-101
Identity = 200/337 (59.35%), Postives = 235/337 (69.73%), Query Frame = 0


Query: 1   MAKLSYMIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEP 60
           M K+S+MI +FM+ M  +VG YD+LLGA YGL G+NL  P+ VV+LC K+ I RVRL   
Sbjct: 1   MVKISHMIGLFMADMFLIVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRLEVS 60

Query: 61  NLDVLKEFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGD 120
           N+DV++ FRG  IDLS  +PN ML +MATN ++VEEWFNT        +   +++   GD
Sbjct: 61  NIDVIEAFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDY--IINYIVVGD 120

Query: 121 KAIPGLHDFILPVMKSLQDLLNAHYLGQRR------------------------------ 180
           KAIPGL D ILPVMKSLQDLLNAHY G+ +                              
Sbjct: 121 KAIPGLDDNILPVMKSLQDLLNAHYFGEAKLTTLVGFNALAVTNPPSSGAFNPAIVENMR 180

Query: 181 ----------SPLMVSVNPYQAYVYDGNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDA 240
                     SPLMVSV  Y  YVY GN SL YAT +E+N++V DG LSYNNLFDEMVDA
Sbjct: 181 GILEFLRGEGSPLMVSVYTYDQYVYGGNRSLGYATLSEQNSLVRDGMLSYNNLFDEMVDA 240

Query: 241 FYAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVY 297
           FYAAIDKENVG+VAIVIGETGWPTCGNY   TP LAA YN NFKSHISSGKGTP+K N+Y
Sbjct: 241 FYAAIDKENVGDVAIVIGETGWPTCGNYNATTPLLAAEYNRNFKSHISSGKGTPRKENIY 300

[Close]

BLAST of Tan0013947 vs. ExPASy TrEMBL
Match: A0A6J1C4V7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 PE=3 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 1.4e-90
Identity = 185/338 (54.73%), Postives = 225/338 (66.57%), Query Frame = 0


Query: 1   MAKLSYMIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEP 60
           MAKL+++I+ FM++M S V   D+LLG  YGL G+N+P P  VV+LC+K+ IRR+RL EP
Sbjct: 1   MAKLAHIIVFFMASM-SAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEP 60

Query: 61  NLDVLKEFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGD 120
           NL+V   F G GID+SF +PN ML +M  N S V EWFNT        F   +++   G+
Sbjct: 61  NLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDF--VINYIIVGN 120

Query: 121 KAIPGLHDFILPVMKSLQDLLNAHYLGQRR------------------------------ 180
           KAIPGL D ILPVMKSLQ+LLNA YLGQ +                              
Sbjct: 121 KAIPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNI 180

Query: 181 -----------SPLMVSVNPYQAYVYDGN-ISLSYATFTEKNTVVYDGTLSYNNLFDEMV 240
                      SPLMVSV PY  Y Y    ISL+YATF  K  VV+DG LSY+NLFD MV
Sbjct: 181 REILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMV 240

Query: 241 DAFYAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPN 297
           D FYAA+DK  VG+V + +GETGWPTCGN  +ATP  A+ YN+NFKSHISSG+GTPK+PN
Sbjct: 241 DPFYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPN 300

[Close]

BLAST of Tan0013947 vs. ExPASy TrEMBL
Match: A0A1S3BQX5 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 2.0e-89
Identity = 181/341 (53.08%), Postives = 229/341 (67.16%), Query Frame = 0


Query: 1   MAKLSYMIIIFMSAM---TSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRL 60
           MAKL  +I+  M+++   TS+V  +D+LLGA YGL+GNNLP P  VV LC K+ IRR+RL
Sbjct: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60

Query: 61  YEPNLDVLKEFRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHR 120
             P +DVL  FRG  IDLSFS+PN MLTDMATN ++VEEWFNT  +     F+  +++  
Sbjct: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFK--INYII 120

Query: 121 RGDKAIPGLHDFILPVMKSLQDLLNAHYLGQRR--------------------------- 180
            GDKA+PGL +FILPVM SLQDLLNA+Y GQ +                           
Sbjct: 121 VGDKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVR 180

Query: 181 --------------SPLMVSVNPYQAYVYDGNISLSYATFTEKNTVVYDGTLSYNNLFDE 240
                         S LMVSV P++ Y  +G +SL+YA   E   VV DG L Y+NLFD 
Sbjct: 181 ENMRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDA 240

Query: 241 MVDAFYAAIDKENVGEVAIVIGETGWPTCG-NYGVATPSLAATYNENFKSHISSGKGTPK 297
           M+DAFYAAI+KE +GEV +V+GETGWPTCG N+ + T  +A  YN  FKSHISSGKGTP+
Sbjct: 241 MMDAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPR 300

[Close]

BLAST of Tan0013947 vs. ExPASy TrEMBL
Match: A0A5D3CFE1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001650 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.3e-88
Identity = 187/334 (55.99%), Postives = 229/334 (68.56%), Query Frame = 0


Query: 9   IIFMSAMTS-VVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLKE 68
           ++FM AM+S VVG Y+ILLGA YGL  NNLP P  V++LC K+ IRR+R+ EPNLDV++ 
Sbjct: 11  LLFMVAMSSIVVGAYNILLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNLDVIES 70

Query: 69  FRGVGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIPGLH 128
           FRG  I++SF + N ++T+MATN++ VEEWF              +++   GDKA+PGL 
Sbjct: 71  FRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDL--TINYIVVGDKAVPGLD 130

Query: 129 DFILPVMKSLQDLLNAHYLGQ--------------------------------------- 188
           + ILPVMKSLQDLLN  +LGQ                                       
Sbjct: 131 NSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVFENMRGILQFLW 190

Query: 189 -RRSPLMVSVNPYQAYVYDGNISLSYATFT---EKNTVV-YDGTLSYNNLFDEMVDAFYA 248
            + SPLM+S+NPY+AY Y+ NISL YATFT   E+N  +  DG LSYNNLFDE+VDAFYA
Sbjct: 191 GQGSPLMLSLNPYEAYAYN-NISLGYATFTPQLEQNPPIRIDGNLSYNNLFDEVVDAFYA 250

Query: 249 AIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVYMEG 297
           AIDK +VG VAI IGETGWPT G    A+P LAATYN NFK+HISSGKGTP KPN+Y+EG
Sbjct: 251 AIDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKGTPMKPNIYIEG 310

[Close]

BLAST of Tan0013947 vs. ExPASy TrEMBL
Match: A0A5A7UKS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001680 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 2.3e-88
Identity = 175/323 (54.18%), Postives = 218/323 (67.49%), Query Frame = 0


Query: 16  TSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLKEFRGVGIDL 75
           TS+V  +D+LLGA YGL+GNNLP P  VV LC K+ IRR+RL  P +DVL  FRG  IDL
Sbjct: 8   TSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRGSNIDL 67

Query: 76  SFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIPGLHDFILPVMK 135
           SFS+PN MLTDMATN ++VEEWFNT  +     F+  +++   GDKA+PGL +FILPVM 
Sbjct: 68  SFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFK--INYIIVGDKAVPGLDNFILPVMM 127

Query: 136 SLQDLLNAHYLGQRR-----------------------------------------SPLM 195
           SLQDLLNA+Y GQ +                                         S LM
Sbjct: 128 SLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALM 187

Query: 196 VSVNPYQAYVYDGNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDAFYAAIDKENVGEVA 255
           VSV P++ Y  +G +SL+YA   E   VV DG L Y+NLFD M+DAFYAAI+KE +GEV 
Sbjct: 188 VSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVT 247

Query: 256 IVIGETGWPTCG-NYGVATPSLAATYNENFKSHISSGKGTPKKPNVYMEGFIESMFNENE 297
           +V+GETGWPTCG N+ + T  +A  YN  FKSHISSGKGTP+KPNVY+EGFI+S+FNEN+
Sbjct: 248 LVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQ 307

[Close]

BLAST of Tan0013947 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 198.7 bits (504), Expect = 6.4e-51
Identity = 118/332 (35.54%), Postives = 181/332 (54.52%), Query Frame = 0


Query: 8   IIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLKE 67
           I++++++  S   T    +G  YGL G+NLP PS+V+ L     I R+R+++PN +VL  
Sbjct: 16  IVLYVNSNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNA 75

Query: 68  FRG-VGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIPG- 127
            RG   I+++  + +  L  +A +   V+ WF  +  +        +     G++ IPG 
Sbjct: 76  LRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADV--NITFITVGNEVIPGP 135

Query: 128 LHDFILPVMKSLQDLLNAH---------------------------------------YL 187
           +   +LPVM+SL +L+ +                                         L
Sbjct: 136 IGPQVLPVMQSLTNLVKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLL 195

Query: 188 GQRRSPLMVSVNPYQAYVYD-GNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDAFYAAI 247
            Q  +P++V++ PY AY  D  NI L YA+F  K+ VV DG+L Y+N+FD + DAF  A+
Sbjct: 196 SQTSTPILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAM 255

Query: 248 DKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVYMEGFI 297
           +KE V  + +V+ ETGWP+ GN    TP++A+TYN NF  HI+SGKGTPK+PN  M GF+
Sbjct: 256 EKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFL 315

[Close]

BLAST of Tan0013947 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 191.8 bits (486), Expect = 7.8e-49
Identity = 112/314 (35.67%), Postives = 169/314 (53.82%), Query Frame = 0


Query: 26  LGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLKEFRG-VGIDLSFSIPNSML 85
           +G  YGL G+NLP PS V+ L     I ++R+++PN +VL   RG   I ++  + +  L
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 86  TDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIPG-LHDFILPVMKSLQDLLNA 145
             ++ +   V+ WF T+           +     G++ IPG +   +LPVM+SL +L+ +
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDI--NIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKS 162

Query: 146 H---------------------------------------YLGQRRSPLMVSVNPYQAYV 205
                                                    L Q  SP++V + PY +Y 
Sbjct: 163 RNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYA 222

Query: 206 YD-GNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDAFYAAIDKENVGEVAIVIGETGWP 265
            D  +I L YATF  +  VV DG+L Y+N+FD + DAF  A++KE V ++ +V+ ETGWP
Sbjct: 223 SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 282

Query: 266 TCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVYMEGFIESMFNENEKPEGESRCYG 297
           + GN  + TP +A TYN NF  HI+SGKGTPK+PN  ++GF+ + FNEN+KP G  + +G
Sbjct: 283 SAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFG 342

[Close]

BLAST of Tan0013947 vs. TAIR 10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 1.1e-47
Identity = 113/336 (33.63%), Postives = 171/336 (50.89%), Query Frame = 0


Query: 4   LSYMIIIFMSAMTSVVGTYDILLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLD 63
           L+Y  + F+ A T++        G  YGL G+NLP PS+V+ L     +  +R+++   D
Sbjct: 19  LNYSNVGFVDAATNI--------GLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTTTD 78

Query: 64  VLKEFRG-VGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKA 123
           VL  FRG   I +   + N  L  ++ +   V  WF T+           +     G++ 
Sbjct: 79  VLNAFRGNRNIGVMVDVKNQDLEALSVSEEAVNTWFVTNIEPYLADV--NITFIAVGNEV 138

Query: 124 IPG-LHDFILPVMKSLQDLLNAH------------------------------------- 183
           IPG +  ++LPVMKSL +++ +                                      
Sbjct: 139 IPGEIGSYVLPVMKSLTNIVKSRSLPILISTTVAMTNLGQSYPPSAGDFMPQAREQLTPV 198

Query: 184 --YLGQRRSPLMVSVNPYQAYVYDG-NISLSYATFTEKNTVVYDGTLSYNNLFDEMVDAF 243
             +L Q  +P++V++ PY AY  D  NI L YA F     VV DG L Y N+FD + DAF
Sbjct: 199 LKFLSQTNTPILVNIYPYFAYAADPINIQLDYAIFNTNKVVVQDGPLGYTNMFDVIFDAF 258

Query: 244 YAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVYM 297
             A++KE V ++ +V+ ETGWP+ GN  + TP +A+ YN NF  H+ SGKGTPK+P   +
Sbjct: 259 VWAMEKEGVKDLPMVVTETGWPSAGNGNLTTPDIASIYNTNFVKHVESGKGTPKRPKSGI 318

[Close]

BLAST of Tan0013947 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 181.4 bits (459), Expect = 1.0e-45
Identity = 112/313 (35.78%), Postives = 161/313 (51.44%), Query Frame = 0


Query: 26  LGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYEPNLDVLKEFRG-VGIDLSFSIPNSML 85
           +G  YGL G+N P PS+V+ L     I ++R+++P  +VL   RG   I+++  + +  L
Sbjct: 34  IGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVLNALRGHRNIEVTVGVRDQDL 93

Query: 86  TDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRRGDKAIPG-LHDFILPVMKSLQDLLNA 145
             ++ N   V+ WF T+           +     G++ IPG +   +LPVM+SL  L+ +
Sbjct: 94  GALSANEEAVKGWFATNIEPYLADV--NIAFITVGNEVIPGPIGPQVLPVMQSLTILVKS 153

Query: 146 ---------------------------------------HYLGQRRSPLMVSVNPYQAYV 205
                                                    L Q  +P++V++ PY  Y 
Sbjct: 154 MNLPISISTVVAMSNLEQSYPPSAGEFTSQAREQLVPVLKLLSQTSTPILVNIYPYFPYA 213

Query: 206 YD-GNISLSYATFTEKNTVVYDGTLSYNNLFDEMVDAFYAAIDKENVGEVAIVIGETGWP 265
            D  NI L YATF  K TVV DG L Y+N+FD + DAF  A++KE V ++ +V+ ETGWP
Sbjct: 214 SDPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 273

Query: 266 TCGNYGVATPSLAATYNENFKSHISSGKGTPKKPNVYMEGFIESMFNENEKPEGESRCYG 297
           + GN  + TP +A TYN NF  HI SGKGTPK+PN  M+GF+ + FNEN+KP G      
Sbjct: 274 SAGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMDGFLFATFNENQKPAG------ 333

[Close]

BLAST of Tan0013947 vs. TAIR 10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 3.4e-44
Identity = 107/330 (32.42%), Postives = 168/330 (50.91%), Query Frame = 0


Query: 7   MIIIFMSAMTSVVGTYDI-------LLGACYGLAGNNLPQPSDVVRLCLKHKIRRVRLYE 66
           + ++  S    ++  Y++       ++G  YGL G+NLP PS+V++      + ++R++E
Sbjct: 8   LFLLLFSCTALIISYYNVDSLSTASVVGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFE 67

Query: 67  PNLDVLKEFRG-VGIDLSFSIPNSMLTDMATNNSIVEEWFNTSRRTLHRRFRHQLHHHRR 126
           PN DVL   RG   I ++  I N  L  +A N   V+ WF+T+           +     
Sbjct: 68  PNKDVLNALRGNRDIGVTVGIKNEDLEALAANKDAVKSWFSTNIDPYIADV--NITFITV 127

Query: 127 GDKAIPG--LHDFILPVMKSLQDLLNA--------------------------------- 186
           G++AIPG      +LPV++SL DL+ +                                 
Sbjct: 128 GNQAIPGDKHGPHVLPVIQSLTDLVKSRNLQISISTTVTTTSLARLKPPSAGVLTPQARQ 187

Query: 187 ------HYLGQRRSPLMVSVNPYQAYVYD-GNISLSYATFTEKNTVVYDGTLSYNNLFDE 246
                   L Q  +P+ V++ PY  +  D  N+ L YA F     VV DG L Y+NLFD 
Sbjct: 188 QLVPVLRLLSQTSTPIFVNIYPYYFHASDPKNVPLEYANFNNDQIVVKDGALKYSNLFDA 247

Query: 247 MVDAFYAAIDKENVGEVAIVIGETGWPTCGNYGVATPSLAATYNENFKSHISSGKGTPKK 287
           + DAF  A++KE V  + +V+ ETGWP+ GN G+ TP+L  TY  NF  H++SGKGTPK+
Sbjct: 248 IFDAFLWAMEKEGVKGLPLVVSETGWPSAGNGGMTTPALQYTYIGNFVKHVASGKGTPKR 307

The following BLAST results are available for this feature:

BLAST of Tan0013947 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49352	8.9e-50	35.54	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49353	1.1e-47	35.67	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O22317	4.8e-43	32.84	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
P07979	1.5e-41	31.95	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]
P52407	4.6e-38	31.21	Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis O...	[more]

BLAST of Tan0013947 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6590489.1	1.7e-101	60.42	putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma s...	[more]
XP_022968751.1	3.7e-101	59.35	probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita maxima]	[more]
XP_038880685.1	9.4e-97	55.79	probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida]	[more]
XP_022136187.1	2.9e-90	54.73	probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia]	[more]
KAG6590491.1	3.8e-90	57.36	putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma s...	[more]

BLAST of Tan0013947 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HVR6	1.8e-101	59.35	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=...	[more]
A0A6J1C4V7	1.4e-90	54.73	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 ...	[more]
A0A1S3BQX5	2.0e-89	53.08	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV...	[more]
A0A5D3CFE1	1.3e-88	55.99	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7UKS6	2.3e-88	54.18	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Tan0013947 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20330.1	6.4e-51	35.54	beta-1,3-glucanase 4	[more]
AT5G20340.1	7.8e-49	35.67	beta-1,3-glucanase 5	[more]
AT5G20390.1	1.1e-47	33.63	Glycosyl hydrolase superfamily protein	[more]
AT1G33220.1	1.0e-45	35.78	Glycosyl hydrolase superfamily protein	[more]
AT5G20560.1	3.4e-44	32.42	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 144..294 e-value: 6.6E-39 score: 134.3
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 213..226
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 26..146 e-value: 2.1E-20 score: 74.9
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 147..296 e-value: 1.9E-47 score: 163.8
None	No IPR available	PANTHER	PTHR32227:SF305	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN-RELATED	coord: 145..294 coord: 18..146
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 145..294 coord: 18..146
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 26..295

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0013947.1	Tan0013947.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0031224	intrinsic component of membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds