Tan0013838 (gene) Snake gourd v1

Overview
NameTan0013838
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionL-ascorbate oxidase
LocationLG09: 61131878 .. 61138625 (-)
RNA-Seq ExpressionTan0013838
SyntenyTan0013838
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAAACCCATGGAGCCATTTTGGAGAGACTTTCGAGTCTTTTTTATTATATTTTATGCTTCAATTTTTAAATTTGGTCTATCTGAGAGTTCTCAAACTAGGAAATTTCAATGGGATGTAGAATACATGTTTTGGTCCCCAGATTGCAAAGAGAACATGGTTATGGGGATCAATGGTCAATTTCCCGGTCCGACCATCAAAGCCAATGCTGGAGACACTGTCGTTGTTAATGTCACTAACAAGCTTTTTACCGAAGGCGTTGTCATTCATTGGCATGGGATTAGGCAAATTGGAACTCCGTGGGCCGATGGCACTGCTGCTATTTCCCAGTGTGCCATCAACCCCGGAGAAACTTTTACTTACACTCTTATAGTTGATAAGGTGAGGTCGTTATGTTGTTTAGCGACTATATCATTTCTATCGATAATAGTTTGTAAAGTTATATAATAAGTGTGTTTTTGTGACAATTTTGGTAATTGTGGTGTATGCAGGCAGGGACATACTTCTATCACGGGCACTATGGAATGCAAAGAATGGCAGGACTTTATGGCTCTTTGATAGTGGATGTCGCAGAAGGTGAAATAGAACCTTTTCATTATGATGAAGAACTCAATTTGTTGCTTAGCGATTGGTGGCATCAAGGTGTTCATGAATTAGAAGTTGGTCTCAGCTCAAGTCCAACTCGTTGGATCGGTGACCCTCAGGTATATGCCACTTGATTAACGCTCGTTTGATAATTATTTGATTTTTAGCTTTTTTTTAATTAAGTTTATAAATACTATTTTTATTCGTAAAATTTTTATTTTGTATCGTCTACTTTCTATCAATGTTTTGAAAAATCAAGTTAAATTTTGAAAACTAAAAACGGTAGTTTGTGAAACATGTTTTTGTTTTTGGAATATGATTAAGAAATCAAATGTTTATTTAAGAAAAATGAAAACCATGGTAGAAATCATGAGAAAACATGTTTAATTTTTAAAAATAAAAAATTATTAATTAAATATCAAATGGTTACCAAACACGTTCCAATTAATAAAAAGGTCCAAAAACAAGTTCAAATTGGATATTTTATATATACCCTATGAACATAGGCGGACCCAAAATACTAATGCATGGGGGGGCGACAAATTATTATTTAATAAATATAGTAAAAAAATTCATCAAAAGATCAATTTGAACACAATATTATGTGGATTTAAAAAATTATCGATAAAAATAGATGGGTGTACAAAAGACTTCCTAACTAAGTTTGCATTACACAGAACTATAAGTATATGCTGTTATTTTTTTAAACCAAATCGTTACCTAATATAGAGTACTAAATTTAAAAATTTTGGAGGCCAATATAAATCTGCCGATGCCTATGAATTTCTTTTTTTAATGGACACAAGTTGGTTTGCTCTCTTGTAAATAAGAGAAGTCGTTTTCTAGATGTGGTAAGTGGTATAGTTATAAACACAATAAAAATACATGAGTTAGGTTGGTTTGGGTTGACCGAGTTATATACTCGAAGTTTTATTTTAAGAAAATAAAGCTCCTCAATCTAAACATCCCCTTAATTTATTTTGGAAAAAAAAATACAACATAAATGTTATTAAGTAAAGGGTTTTTTTTATGTGCATGTTAAATTACAAATTGGTCTCTACGGTTTTGGAAGAAGTTAGAATTTAGTTCCTAGAGTTAAGTTAGAATTTTGTCCATATAATTTGATAAAACCTCGTAAACAGTTTTATATATAGTTAAAAACCATCAATGATAGATTGAGTACATGACGATCAGCTCTATTCATGTATCAATAAGATGGTCCTTTGTATTTAGTTGTGAGTTAAATTAAATGGTTGATTGGATAAAAAAATGTTGAAAATTGACAATTTAATGCAATTTTTTTGAAGGACAACTTATTTTTTAATCCAGTCAACTACTTAACATACACAATCAGCGAAAAATAAAACATCGTCTCATTGACACATGAATAGTCAACTACTATGTACGCAATTTTTTAATAACTATTAATGATATGAACTATTTGTGATGTTTTATCAAACTAAAAAGATAAAATTCTAACTTTTAATAAACTATGGACTAAATTTTAACCTTTTTTCGATTAAATGAATAAATAAGAGAAAATTGTGTAGGTAGGTGAGAAAGTTGATCTAGAAATGATGAATTATTATTGCATGCAGAGTTTGCTGATAAATGGAAGAGGTCAATTTGGCTGCTCACTTGCAGCTAAGTACGATAGTAGCAGTTCGATGAAGCAGTGTGAGTTAAAAGGAAATGAACAATGCTCACCCTTTATCCTCAAAGTTGAACCAAATAAGACTTATAGACTCAGAATCGCAAGCACCACTTCATTGGCTTCACTCAATGTCGCCATTGGAGTAAGTTTCTGTTGCTCAATCTCGGAGAAATTATAAGAATTGAGCATATTTGAAAACTTATTACAAAAATCGAGTAGTTTTGAAAACTATTTACAAAAAACATGTTAATTTTTTGTCCTTATTACGATTTAAATCACCAACCAAATTTTTCTTGACTTTTCACCTTCATTTCTATTCAGCATTCCTTTCCGAAATTTTCTCTCATTTTTTCAATTCTTCTTATTCTTCTCCTTGTTTTGGTGTGCCATTTGTTTTCTTTTATTATTTTCTCATACACCTTCAAAAATCAAAATATTTCATCTCAAATCTTTTTCACTGCGTTATATTTCATCCCAAATCATTTTCACTGCATTTTCAACATATTTAGTCTAATTGTCACTCAAAAAGTAAGTTATCTAATTTTTTAATTTATTTAGTCTAATTTTAATTTTTTTTATGATTAATGATTGGTTATTCTTGTTTATTTTCTTTTATTATTTTCTCATACACCTTCAACAATACTTGATATTTTTGTATAAAACTCTACCAGTTCTAAAATTTTGCTATATTTAAAAAGAATGTGAAAAATACCTAAATAATTAAATGTTATATTTAAAATAAAGATGAGAAATTCCCTTTGAAAAACGTGTTCCAAAAAACGCATTCCAATAAAGACAAAAAATTTACCCTATTTATATAAATAGTTTTAAAGGGGGCATATTTCTTGAAAATAGTTTTAAAAAATACCCTATTCTAGCATTTAGCCCCTCAATCTCACTTCTGTTTCTATGTCAACTAAAATGGTTGATTGGAAAATGCTTTTCTTTCTCATTTTTAGACCTTATTTTTTAGTATCTAACTTATTTTCTTAAGGAAAATACTTGATTTGGTTATTCTATCGATGTATTTTGCAACCTACTTAATAACTAAAAAAATTGTGATTGAAAAATGGGCGCTTAGGTCTGCGGCTCATACAACACTCAAATTGTGCCCTTTTCTTAATTGTTGTTGATTTGAAATACACTCAAATTGTGCCCTTTTCTTAATTGTTGTTGATTTCAAATATAGAATCACAATATGATAGTGGTAGAATGTGACGGAAACTACGTCGAGCCATTCACAACTCCCAGTATCGACATTCACTCCGGCGAGAGCTACTCCGTCCTCATTGCCACCAACCAAGACCCTTCGAAAAACTATTGGATCTCCACCGCGGTCACCGGACAACAACCCAACACCCCTCCTGGCCTTACCATCCTCAACTACCTCCCCACTCCGGCCACCGATCTCCCCATCTCTCCGCCGCAGTGGGACGACATGACCCGCCTCAAGAACTTCGCCAAACAAATCACCGCCACCGCCACCTCTCCAAAGCCGCCACAGAACTTCACCCGCCGGATCTTCCTCCTCAACACTCAAAACAAAATCAACGGCGTAGAGAAATGGGCCCTCAACAACGTATCGTTAACCCTCCCAACGACGCCGTATTTGGGCGCCATCAAATTCTGGTTGAACCAAGCCTTCGACCAAAGCAACCCGCCGGAGACTTTCCCAGAAGGCTACAATATCGACGTCCCTCCGGTGAACAGCGCCACCACCGTCAACAACGCGATTTATCGACTGGAATTGGGGTCGACGGTGGATTTTGTGCTTCAAAATACGAACATGTTGCATGAAAATGAAAGCGAGAATCACCCATGGCATTTGTGTTGGGAAAAAAACCTCCCACCAGTAGGAATAAACAGAAAGAACTGAGAAATTAAAAGAAACCAACAAAATAGAGACACAAGAATTTAACGTGGAAAAACTCCAAACCCGGAGAAAAAAAAACCACCAACAGAAAGAAATTCCACTATGTGAAAAATTGTTACAATCACATAGAATTCTCTCCCCGACCCCAATTACAAAAACACTCTCCCAAAACTCTATACCACTCACACCCTTCTTCCCACTCTCAGACCAGAGAATATAAAAAAGGGAATTTAACTAGAGCTAGTACACCAAGTTTAAAGTGTTTCTAACTGGGATGCTTTGAAACCAAAGCACATAGGCTCCTTTTATAGTGCCAGGAGCCCATCACTTCCTTGCATCTTTCAGATGTGGGACAATTTCACTTCTAATATATTGCCAAAAAACCCAACAATTTCCACCTTGACAAGATATTTGAAGAATCCACATCTCTTGCTGCATCATTGTCTTTACCGACAATCATAATCCTCAACAGAGAACTATAACCTACTCCACGAACACTTGGAATGTCACCTTCCAAGACTTTTCTTTTTCGTCACATGCCGATATACCCAGCTGAAATTTATGGTGCAACTTCCACCTACTTGGCTCACCGGAAGTTCTTCAGCCATCGACATAACTTCCACCACACACCTTGCATAGCCGCCAAGTCAATGCCCGTGTGCAAACTTGTGGAACCGCTAACATCACATCCTCTATCATGGAAAAGCGGACATACCATAAGGATGATTTTTGTCTTTCTTCAGAAGATGTCACCATCTCCCCTGACGAGAGAGACCACGTTAGCATCCAACTCAGAGCCTTTTGACGAACTTGCTCTGTTAGGACAATTCTTTTTCATGTGTCCAGATCGACCGTCATCACAGCAACCTTTCTTTTGCACTGCAAGGAAGTGATGGGCTCCTGGCACTATAAAAGGAGCCTATGGCTTTGGTTTCAAAGCATCCCAGTTAGAAACACTTTAAACTTGGTGTACTAGCTCTAGTTAAATTCCCACCTTGACAAGATATTTGAAGAATCCACATCTCTTGCTGCATCATTGTCTTTACCGACAATCATAATCCTCAACAGAGAACTATAACCTACTCCACCATAAAAGTAGAACACTTGGAATGTCACCTTCCAAGACTTTTCTTTTTCGTCACATGCCGATATACCCAGCTGAAATTTATGGTGCAACTTCCACCTACTTGGCTCACCGGAAGTTCTTCAGCCATCGACATAACTTCCACCACACACCTTGCATAGCCGCCAAGTCAATGCCCGTGTGCAAACTTGTGGAACCGCTAACATCACATCCTCTATCATGGCAAAGCGGACATACCATAAGGATGATTTTTGTCTTTCTTCAGAAGATGTCACCATCTCCCCTGACGAGAGAGAGACCACGTTAGCATCCAACTCGTGAGCCTTTGACGAACTTGCTCTCTTAGGACAATTCTTTTCATGTGTCCAGGATCGACCGGCCATCACAAAGAACCTTTCTTTTGCACGAAGTCTTTCTTCTTCTTCTTCCAATTCCAGATTGTCATGTTAGACTTCTTAGAATCTTTGCTGGAACCACCACTGGACTCCGCTGGATCACCATCACCGCTTAACCTTTGAGGAACACCACAACTTGGTCTCCCTTCAAAGCCTTGTGTAACTCGGGATTAGATCAAAGACATCTTTGACTTGCACTTGCCACAAGCCAAAGTTCATCTTTCCACAAATTTCTCCACATCAATCTTTATCTGACTCATAAAGCTTGACATATCTCGCTTCCCTCCTAGTGAATCAGCAAATAGTGAACAACTATAACCCGCTTTCAAGAAATATTTTCATGTGGAAGCTCGATAATGTCTTTGTGCACCACAGAGCATACTCTAAACTCAAGAAACTACAAACCTAAAGCTCGATACCACTTGTTGGGAAAAAAAAACCTCCCACCACTAGGAATAAGCAGAAATAAACCGAGAAATTAAAAGATACCAACAAAATAAGAGACAAAAGAATTTACGTGGAAAAACTCCAAACCCGGAGAAAAAAACCACGAACAGAAAGAAATTTCCACTATGTGAAAAATTGTTACAATCACATAGAATTCTCTCCCCGACCCCAATTACAAAAACACTCTTCCAAAACTCTATACCACTCACACCCTTCTTCCCACTCCTGATCGTAGAATATAAAAATGGAATTTAACTAGAGCCTAAGACACCAAGCTTTAAAAGTGTTTAACCTGGGATCTTTGAAACTAAAGCCCATGCGCTCCTTTTATAGTGTCGAGCCTCCATCACTTCTCTCAGATCTTTCAGGATGTGGGACAATTTCACTTCTAATATATTGCCAAAAAACTCAACAATTTGCATGGACATGATTTCTGAGTTTTGGGTTTTGGAGAAGGGAAATTCTCGGAGGAGGATGAGAAGAATCTGAACTTGAAGAACCCGCCGGTGAAGAACACGGTGGTGATTTTTCCATTTGGGTGGACGGCGGTGAGGTTTGTGGCGGATAATCCTGGAGTTTGGATATTTCATTGCCATATTGAACCTCATTTGCATATGGGAATGGGTATTATTTTTGCTGAAGGTGTTGATAGAGTTGGTGATATTCCTAAGAATGCTCTCTCTTGTGGCCTCACTGGACAAATGTAG

mRNA sequence

ATGGGTAAACCCATGGAGCCATTTTGGAGAGACTTTCGAGTCTTTTTTATTATATTTTATGCTTCAATTTTTAAATTTGGTCTATCTGAGAGTTCTCAAACTAGGAAATTTCAATGGGATGTAGAATACATGTTTTGGTCCCCAGATTGCAAAGAGAACATGGTTATGGGGATCAATGGTCAATTTCCCGGTCCGACCATCAAAGCCAATGCTGGAGACACTGTCGTTGTTAATGTCACTAACAAGCTTTTTACCGAAGGCGTTGTCATTCATTGGCATGGGATTAGGCAAATTGGAACTCCGTGGGCCGATGGCACTGCTGCTATTTCCCAGTGTGCCATCAACCCCGGAGAAACTTTTACTTACACTCTTATAGTTGATAAGGCAGGGACATACTTCTATCACGGGCACTATGGAATGCAAAGAATGGCAGGACTTTATGGCTCTTTGATAGTGGATGTCGCAGAAGGTGAAATAGAACCTTTTCATTATGATGAAGAACTCAATTTGTTGCTTAGCGATTGGTGGCATCAAGGTGTTCATGAATTAGAAGTTGGTCTCAGCTCAAGTCCAACTCGTTGGATCGGTGACCCTCAGAGTTTGCTGATAAATGGAAGAGGTCAATTTGGCTGCTCACTTGCAGCTAAGTACGATAGTAGCAGTTCGATGAAGCAGTGTGAGTTAAAAGGAAATGAACAATGCTCACCCTTTATCCTCAAAGTTGAACCAAATAAGACTTATAGACTCAGAATCGCAAGCACCACTTCATTGGCTTCACTCAATGTCGCCATTGGAAATCACAATATGATAGTGGTAGAATGTGACGGAAACTACGTCGAGCCATTCACAACTCCCAGTATCGACATTCACTCCGGCGAGAGCTACTCCGTCCTCATTGCCACCAACCAAGACCCTTCGAAAAACTATTGGATCTCCACCGCGGTCACCGGACAACAACCCAACACCCCTCCTGGCCTTACCATCCTCAACTACCTCCCCACTCCGGCCACCGATCTCCCCATCTCTCCGCCGCAGTGGGACGACATGACCCGCCTCAAGAACTTCGCCAAACAAATCACCGCCACCGCCACCTCTCCAAAGCCGCCACAGAACTTCACCCGCCGGATCTTCCTCCTCAACACTCAAAACAAAATCAACGGCGTAGAGAAATGGGCCCTCAACAACGTATCGTTAACCCTCCCAACGACGCCGTATTTGGGCGCCATCAAATTCTGGTTGAACCAAGCCTTCGACCAAAGCAACCCGCCGGAGACTTTCCCAGAAGGCTACAATATCGACGTCCCTCCGGTGAACAGCGCCACCACCGTCAACAACGCGATTTATCGACTGGAATTGGGGTCGACGGTGGATTTTGTGCTTCAAAATACGAACATGTTGCATGAAAATGAAAGCGAGAATCACCCATGGCATTTGTTTTTGGGTTTTGGAGAAGGGAAATTCTCGGAGGAGGATGAGAAGAATCTGAACTTGAAGAACCCGCCGGTGAAGAACACGGTGGTGATTTTTCCATTTGGGTGGACGGCGGTGAGGTTTGTGGCGGATAATCCTGGAGTTTGGATATTTCATTGCCATATTGAACCTCATTTGCATATGGGAATGGGTATTATTTTTGCTGAAGGTGTTGATAGAGTTGGTGATATTCCTAAGAATGCTCTCTCTTGTGGCCTCACTGGACAAATGTAG

Coding sequence (CDS)

ATGGGTAAACCCATGGAGCCATTTTGGAGAGACTTTCGAGTCTTTTTTATTATATTTTATGCTTCAATTTTTAAATTTGGTCTATCTGAGAGTTCTCAAACTAGGAAATTTCAATGGGATGTAGAATACATGTTTTGGTCCCCAGATTGCAAAGAGAACATGGTTATGGGGATCAATGGTCAATTTCCCGGTCCGACCATCAAAGCCAATGCTGGAGACACTGTCGTTGTTAATGTCACTAACAAGCTTTTTACCGAAGGCGTTGTCATTCATTGGCATGGGATTAGGCAAATTGGAACTCCGTGGGCCGATGGCACTGCTGCTATTTCCCAGTGTGCCATCAACCCCGGAGAAACTTTTACTTACACTCTTATAGTTGATAAGGCAGGGACATACTTCTATCACGGGCACTATGGAATGCAAAGAATGGCAGGACTTTATGGCTCTTTGATAGTGGATGTCGCAGAAGGTGAAATAGAACCTTTTCATTATGATGAAGAACTCAATTTGTTGCTTAGCGATTGGTGGCATCAAGGTGTTCATGAATTAGAAGTTGGTCTCAGCTCAAGTCCAACTCGTTGGATCGGTGACCCTCAGAGTTTGCTGATAAATGGAAGAGGTCAATTTGGCTGCTCACTTGCAGCTAAGTACGATAGTAGCAGTTCGATGAAGCAGTGTGAGTTAAAAGGAAATGAACAATGCTCACCCTTTATCCTCAAAGTTGAACCAAATAAGACTTATAGACTCAGAATCGCAAGCACCACTTCATTGGCTTCACTCAATGTCGCCATTGGAAATCACAATATGATAGTGGTAGAATGTGACGGAAACTACGTCGAGCCATTCACAACTCCCAGTATCGACATTCACTCCGGCGAGAGCTACTCCGTCCTCATTGCCACCAACCAAGACCCTTCGAAAAACTATTGGATCTCCACCGCGGTCACCGGACAACAACCCAACACCCCTCCTGGCCTTACCATCCTCAACTACCTCCCCACTCCGGCCACCGATCTCCCCATCTCTCCGCCGCAGTGGGACGACATGACCCGCCTCAAGAACTTCGCCAAACAAATCACCGCCACCGCCACCTCTCCAAAGCCGCCACAGAACTTCACCCGCCGGATCTTCCTCCTCAACACTCAAAACAAAATCAACGGCGTAGAGAAATGGGCCCTCAACAACGTATCGTTAACCCTCCCAACGACGCCGTATTTGGGCGCCATCAAATTCTGGTTGAACCAAGCCTTCGACCAAAGCAACCCGCCGGAGACTTTCCCAGAAGGCTACAATATCGACGTCCCTCCGGTGAACAGCGCCACCACCGTCAACAACGCGATTTATCGACTGGAATTGGGGTCGACGGTGGATTTTGTGCTTCAAAATACGAACATGTTGCATGAAAATGAAAGCGAGAATCACCCATGGCATTTGTTTTTGGGTTTTGGAGAAGGGAAATTCTCGGAGGAGGATGAGAAGAATCTGAACTTGAAGAACCCGCCGGTGAAGAACACGGTGGTGATTTTTCCATTTGGGTGGACGGCGGTGAGGTTTGTGGCGGATAATCCTGGAGTTTGGATATTTCATTGCCATATTGAACCTCATTTGCATATGGGAATGGGTATTATTTTTGCTGAAGGTGTTGATAGAGTTGGTGATATTCCTAAGAATGCTCTCTCTTGTGGCCTCACTGGACAAATGTAG

Protein sequence

MGKPMEPFWRDFRVFFIIFYASIFKFGLSESSQTRKFQWDVEYMFWSPDCKENMVMGINGQFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFTYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGVHELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILKVEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIATNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISPPQWDDMTRLKNFAKQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQAFDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPWHLFLGFGEGKFSEEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFHCHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLTGQM
Homology
[Close]
BLAST of Tan0013838 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 818.5 bits (2113), Expect = 4.6e-236
Identity = 382/577 (66.20%), Postives = 454/577 (78.68%), Query Frame = 0 

Query: 1   MGKPMEPFWRDFRVFFIIFYASIFKFGL-SESSQTRKFQWDVEYMFWSPDCKENMVMGIN 60
           MGK  EP   +F + F  F+  I  FG+ SE SQ R ++W+VEYMFW+PDC EN+VMGIN
Sbjct: 4   MGKAREP---NFLILF--FFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63

Query: 61  GQFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGET 120
           GQFPGPTI+ANAGDTVVV + NKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGET
Sbjct: 64  GQFPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 121 FTYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQG 180
           F Y   VD  GT+FYHGH GMQR AGLYGSLIVD  +G+ EPFHYD E+NLLLSDWWHQ 
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183

Query: 181 VHELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFIL 240
           +H+ EVGLSS P RWIG+PQ++L+NGRGQF CS+AAKYD  S+++ C+LKG+E C+P+I 
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYD--SNLEPCKLKGSEPCAPYIF 243

Query: 241 KVEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLI 300
            V P KTYR+RIASTT+LA+LN AIGNH ++VVE DGNYV+PF T  IDI+SGESYSVLI
Sbjct: 244 HVMPKKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLI 303

Query: 301 ATNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNF 360
            T+Q+PS+NYW+S    G+ PNTPPGLT+LNYLP   + LP SP    P WDD  R KNF
Sbjct: 304 TTDQNPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNF 363

Query: 361 AKQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQ 420
             +ITA   SPKPP    RRIFLLNTQN ING  KWA+N+VSL LP TPYLGA+KF L  
Sbjct: 364 TYRITAAMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLH 423

Query: 421 AFDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHP 480
           AFDQ+ PPE FPE Y+ID PP N  T + N +Y+ ++G  VD +LQN NM+ EN SE HP
Sbjct: 424 AFDQNPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHP 483

Query: 481 WHL------FLGFGEGKFSEEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 540
           WHL       LG+G+GKF+ E+E +LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 484 WHLHGHDFWVLGYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 543

Query: 541 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLTGQ 567
           CHIEPHLHMGMG++FAEGV++VG IP  AL+CG T +
Sbjct: 544 CHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAK 573

[Close]
BLAST of Tan0013838 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 1.7e-235
Identity = 387/575 (67.30%), Postives = 454/575 (78.96%), Query Frame = 0 

Query: 6   EPFW-RDFRVFFIIFYASIFKFGLSESS----QTRKFQWDVEYMFWSPDCKENMVMGING 65
           +PF+ + F  F ++  + IF FG++ S     + + ++WDVEYMFWSPDC EN+VMGING
Sbjct: 7   KPFFPKPFLSFLVL--SIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGING 66

Query: 66  QFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETF 125
           +FPGPTI+ANAGD VVV +TNKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGETF
Sbjct: 67  EFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETF 126

Query: 126 TYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGV 185
           TY  +VDKAGTYFYHGH GMQR AGLYGSLIVD  EG  EPFHYDEE+NLLLSDWWHQ V
Sbjct: 127 TYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSV 186

Query: 186 HELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILK 245
           H+ EVGLSS P RWIG+PQS+LING+GQF CS+AAKY  +  +KQCEL G E+C+PFIL 
Sbjct: 187 HKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKY--NQGLKQCELSGKEKCAPFILH 246

Query: 246 VEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIA 305
           V+P KTYR+RIASTT+LASLN AIGNH ++VVE DGNYV+PF T  IDI+SGESYSVLI 
Sbjct: 247 VQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLIT 306

Query: 306 TNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNFA 365
           T+Q+P +NYW+S  V  + P TPPGLT+LNYLP  A+ LPISP    P W+D  R KNF 
Sbjct: 307 TDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFT 366

Query: 366 KQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQA 425
            +I A   SPKPP  + RR+FLLNTQN+ING  KWA+NNVSL LP TPYL A+K  LN A
Sbjct: 367 FRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTA 426

Query: 426 FDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPW 485
           F+Q+ PPETFP  Y+I+ PP N  TT  N +Y+  +G TVD +LQN NML+ N SE HPW
Sbjct: 427 FNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPW 486

Query: 486 HL------FLGFGEGKF-SEEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 545
           HL       LG+GEGKF + EDEK LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 487 HLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 546

Query: 546 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLT 565
           CHIEPHLHMGMG++FAEGV  VG IP  AL+CG T
Sbjct: 547 CHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGST 577

[Close]
BLAST of Tan0013838 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 4.7e-233
Identity = 368/545 (67.52%), Postives = 438/545 (80.37%), Query Frame = 0 

Query: 32  SQTRKFQWDVEYMFWSPDCKENMVMGINGQFPGPTIKANAGDTVVVNVTNKLFTEGVVIH 91
           SQ R ++W+VEYMFW+P+C EN+VMGINGQFPGPTI+ANAGD+VVV +TNKL TEGVVIH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 92  WHGIRQIGTPWADGTAAISQCAINPGETFTYTLIVDKAGTYFYHGHYGMQRMAGLYGSLI 151
           WHGI Q GTPWADGTA+ISQCAINPGETF Y   VD  GT+FYHGH GMQR AGLYGSLI
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 152 VDVAEGEIEPFHYDEELNLLLSDWWHQGVHELEVGLSSSPTRWIGDPQSLLINGRGQFGC 211
           VD  +G+ EPFHYD E+NLLLSDWWHQ +H+ EVGLSS P RWIG+PQ++L+NGRGQF C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180

Query: 212 SLAAKYDSSSSMKQCELKGNEQCSPFILKVEPNKTYRLRIASTTSLASLNVAIGNHNMIV 271
           S+AAKYD  S+++ C+LKG+E C+P+I  V P KTYR+RIASTT+LA+LN AIGNH ++V
Sbjct: 181 SIAAKYD--SNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLV 240

Query: 272 VECDGNYVEPFTTPSIDIHSGESYSVLIATNQDPSKNYWISTAVTGQQPNTPPGLTILNY 331
           VE DGNYV+PF T  IDI+SGESYSVLI T+Q+PS+NYW+S     + PNTPPGLT+LNY
Sbjct: 241 VEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNY 300

Query: 332 LPTPATDLPISP----PQWDDMTRLKNFAKQITATATSPKPPQNFTRRIFLLNTQNKING 391
           LP   + LP SP    P WDD  R KNF  +ITA   SPKPP  F RRIFLLNTQN ING
Sbjct: 301 LPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVING 360

Query: 392 VEKWALNNVSLTLPTTPYLGAIKFWLNQAFDQSNPPETFPEGYNIDVPPVNSATTVNNAI 451
             KWA+N+VSL LP TPYLGA+K+ L  AFDQ+ PPE FPE Y+ID PP N  T + N +
Sbjct: 361 YVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGV 420

Query: 452 YRLELGSTVDFVLQNTNMLHENESENHPWHL------FLGFGEGKFSEEDEKNLNLKNPP 511
           Y+ ++G  VD +LQN NM+ EN SE HPWHL       LG+G+GKFS E+E +LNLKNPP
Sbjct: 421 YQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAEEESSLNLKNPP 480

Query: 512 VKNTVVIFPFGWTAVRFVADNPGVWIFHCHIEPHLHMGMGIIFAEGVDRVGDIPKNALSC 567
           ++NTVVIFP+GWTA+RFVADNPGVW FHCHIEPHLHMGMG++FAEGV++VG IP  AL+C
Sbjct: 481 LRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALAC 540

[Close]
BLAST of Tan0013838 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 4.6e-220
Identity = 364/567 (64.20%), Postives = 437/567 (77.07%), Query Frame = 0 

Query: 14  VFFIIFYASIFKFGLSES---SQTRKFQWDVEYMFWSPDCKENMVMGINGQFPGPTIKAN 73
           +FF +    + +   S S   ++TR F+WDVEY+ WSPD +E++VMGINGQFPGPTI+A 
Sbjct: 7   LFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAK 66

Query: 74  AGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFTYTLIVDKAG 133
           AGDTV V++TNKL TEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETF Y   VDKAG
Sbjct: 67  AGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAG 126

Query: 134 TYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGVHELEVGLSSS 193
           TYFYHGHYGMQR AGLYGSLIV+V EGE EPFHYD E NLLLSDWWH+G HE EV LSS+
Sbjct: 127 TYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSSN 186

Query: 194 PTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILKVEPNKTYRLR 253
           P RWIG+PQ+LL+NGRGQ+ CSLAA++ S   + QC+L+G EQ +P IL+V PNK YRLR
Sbjct: 187 PLRWIGEPQTLLLNGRGQYNCSLAARF-SKPPLPQCKLRGGEQYAPQILRVRPNKIYRLR 246

Query: 254 IASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIATNQDPSKNYW 313
           +ASTT+L SL++AIG H M+VVE DGNYV+PF+   +DI+SGESYSVL  T+QDP+KNYW
Sbjct: 247 VASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYW 306

Query: 314 ISTAVTGQQPNTPPGLTILNYLPTPATDLPISPPQ----WDDMTRLKNFAKQITATATSP 373
           IS  V G++P TP GLT+LNYLP  A+  P  PP     W+D    K+F+ +I A   SP
Sbjct: 307 ISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSP 366

Query: 374 KPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQAFDQSNPPETF 433
           KPP    RRI LLNTQNKI+G  KWA+NNVSL LPT  YLG+I++ +N AFD   PP+ F
Sbjct: 367 KPPPQNHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGIN-AFDTKPPPDNF 426

Query: 434 PEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPWHL------FL 493
           P+ Y++     NS +T  N +Y L+  +T+D +LQN N L ++ SE HPWHL       L
Sbjct: 427 PKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVL 486

Query: 494 GFGEGKFSEEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFHCHIEPHLHMGM 553
           G+GEGKFSE+D K  NLKNPP++NT VIFPFGWTA+RFV DNPGVW FHCHIEPHLHMGM
Sbjct: 487 GYGEGKFSEKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMGM 546

Query: 554 GIIFAEGVDRVGDIPKNALSCGLTGQM 568
           G+IFAEGV  V  IPK AL+CGLTG+M
Sbjct: 547 GVIFAEGVHLVKKIPKEALACGLTGKM 571

[Close]
BLAST of Tan0013838 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 4.0e-208
Identity = 334/547 (61.06%), Postives = 415/547 (75.87%), Query Frame = 0 

Query: 31  SSQTRKFQWDVEYMFWSPDCKENMVMGINGQFPGPTIKANAGDTVVVNVTNKLFTEGVVI 90
           S+  R++ W+VEY F  PDCKE MVM +NGQFPGPTI A AGDT+VV++TNKL TEG+VI
Sbjct: 17  SAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGLVI 76

Query: 91  HWHGIRQIGTPWADGTAAISQCAINPGETFTYTLIVDKAGTYFYHGHYGMQRMAGLYGSL 150
           HWHGIRQ+G+PWADG A ++QCAI+PGETFTY   VDK GT+FYHGHYGMQR AGLYGSL
Sbjct: 77  HWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGLYGSL 136

Query: 151 IVDVAEGEIEPFHYDEELNLLLSDWWHQGVHELEVGLSSSPTRWIGDPQSLLINGRGQFG 210
           I+DVA+G+ EP  YD E NLLLSDWWH+ V   E+GLSS P RWIG+ QS+LINGRGQF 
Sbjct: 137 IIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQSILINGRGQFN 196

Query: 211 CSLAAKYDSSSSMKQCELKGNEQCSPFILKVEPNKTYRLRIASTTSLASLNVAIGNHNMI 270
           CSLAA++ SS+S+  C  K  +QC+P  L VEPNKTYR+R+AS+T+LASLN A+  H ++
Sbjct: 197 CSLAAQF-SSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQGHKLV 256

Query: 271 VVECDGNYVEPFTTPSIDIHSGESYSVLIATNQDPSKNYWISTAVTGQQPNTPPGLTILN 330
           VVE DGNY+ PFTT  IDI+SGE+YSVL+ T+QDPS+NY+I+  V G++PNTPP LT+LN
Sbjct: 257 VVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPALTVLN 316

Query: 331 YLPTPATDLPISP----PQWDDMTRLKNFAKQITATATSPKPPQNFTRRIFLLNTQNKIN 390
           Y+  P++ LP SP    P+W+D  R KNF+K+I A   SP PP+ F  R+ LLNTQN I 
Sbjct: 317 YVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSPPETFDERLILLNTQNLIE 376

Query: 391 GVEKWALNNVSLTLPTTPYLGAIKFWLNQAFDQSNPPETFPEGYNIDVPPVNSATTVNNA 450
           G  KWA+NNVSL +P TPYLG++K+ L   F++S+PP+ +P  Y+I  PP N      N 
Sbjct: 377 GFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMTPPRNRNAKQGNV 436

Query: 451 IYRLELGSTVDFVLQNTNMLHENESENHPWHL------FLGFGEGKFSE-EDEKNLNLKN 510
                   TVD +LQN N L+ N SE HPWHL       LG+GEGKF    DEK  NLKN
Sbjct: 437 SCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDEKTYNLKN 496

Query: 511 PPVKNTVVIFPFGWTAVRFVADNPGVWIFHCHIEPHLHMGMGIIFAEGVDRVGDIPKNAL 567
           PP++NTV ++P+GWTA+RFV DNPGVW FHCHIEPHLHMGMG++FAEG++R+G +P  AL
Sbjct: 497 PPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDEAL 556

[Close]
BLAST of Tan0013838 vs. NCBI nr
Match: XP_022929646.1 (L-ascorbate oxidase [Cucurbita moschata])

HSP 1 Score: 828.2 bits (2138), Expect = 4.4e-236
Identity = 385/577 (66.72%), Postives = 456/577 (79.03%), Query Frame = 0 

Query: 1   MGKPMEPFWRDFRVFFIIFYASIFKFGL-SESSQTRKFQWDVEYMFWSPDCKENMVMGIN 60
           MGK  EP   +F V F  F+  I  FG+ SE SQ R ++W+VEYMFW+PDC EN+VMGIN
Sbjct: 4   MGKAREP---NFLVLF--FFGLILGFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63

Query: 61  GQFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGET 120
           GQFPGPTI+ANAGDTVVV +TNKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGET
Sbjct: 64  GQFPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 121 FTYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQG 180
           F Y   VD  GT+FYHGH GMQR AGLYGSLIVD  +G+ EPFHYD E+NLLLSDWWHQ 
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183

Query: 181 VHELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFIL 240
           +H+ EVGLSS P RWIG+PQ++L+NGRGQF CS+AAKYD  S+++ C+LKG+E C+P+I 
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYD--SNLEPCKLKGSEPCAPYIF 243

Query: 241 KVEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLI 300
            V P KTYR+RIASTT+LA+LN AIGNH ++VVE DGNYV+PF T  IDI+SGESYSVLI
Sbjct: 244 HVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLI 303

Query: 301 ATNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNF 360
            T+Q+PS+NYW+S     + PNTPPGLT+LNYLP   + LP SP    P WDD  R KNF
Sbjct: 304 TTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNF 363

Query: 361 AKQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQ 420
             +ITA   SPKPP  F RRIFLLNTQN ING  KWA+N+VSL LP TPYLGA+K+ L  
Sbjct: 364 TYRITAAMGSPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLH 423

Query: 421 AFDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHP 480
           AFDQ+ PPE FPE Y+ID PP N  T + N +Y+ ++G  VD +LQN NM+ EN SE HP
Sbjct: 424 AFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHP 483

Query: 481 WHL------FLGFGEGKFSEEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 540
           WHL       LG+G+GKFS E+E +LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 484 WHLHGHDFWVLGYGDGKFSAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 543

Query: 541 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLTGQ 567
           CHIEPHLHMGMG++FAEGVD+VG IP  AL+CG+T +
Sbjct: 544 CHIEPHLHMGMGVVFAEGVDKVGRIPTKALACGVTAK 573

[Close]
BLAST of Tan0013838 vs. NCBI nr
Match: KAG6588158.1 (L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022051.1 L-ascorbate oxidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 827.4 bits (2136), Expect = 7.5e-236
Identity = 385/577 (66.72%), Postives = 456/577 (79.03%), Query Frame = 0 

Query: 1   MGKPMEPFWRDFRVFFIIFYASIFKFGL-SESSQTRKFQWDVEYMFWSPDCKENMVMGIN 60
           MGK  EP   +F V F  F+  I  FG+ SE SQ R ++W+VEYMFW+PDC EN+VMGIN
Sbjct: 4   MGKAREP---NFLVLF--FFGLILGFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63

Query: 61  GQFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGET 120
           GQFPGPTI+ANAGDTVVV +TNKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGET
Sbjct: 64  GQFPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 121 FTYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQG 180
           F Y   VD  GT+FYHGH GMQR AGLYGSLIVD  +G+ EPFHYD E+NLLLSDWWHQ 
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183

Query: 181 VHELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFIL 240
           +H+ EVGLSS P RWIG+PQ++L+NGRGQF CS+AAKYD  S+++ C+LKG+E C+P+I 
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYD--SNLEPCKLKGSEPCAPYIF 243

Query: 241 KVEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLI 300
            V P KTYR+RIASTT+LA+LN AIGNH ++VVE DGNYV+PF T  IDI+SGESYSVLI
Sbjct: 244 HVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLI 303

Query: 301 ATNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNF 360
            T+Q+PS+NYW+S     + PNTPPGLT+LNYLP   + LP SP    P WDD  R KNF
Sbjct: 304 TTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNF 363

Query: 361 AKQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQ 420
             +ITA   SPKPP  F RRIFLLNTQN I+G  KWA+N+VSL LP TPYLGA+K+ L  
Sbjct: 364 TYRITAAMGSPKPPVKFNRRIFLLNTQNVISGYVKWAINDVSLALPPTPYLGAMKYNLLH 423

Query: 421 AFDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHP 480
           AFDQ+ PPE FPE Y+ID PP N  T + N +Y+ ++G  VD +LQN NM+ EN SE HP
Sbjct: 424 AFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHP 483

Query: 481 WHL------FLGFGEGKFSEEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 540
           WHL       LG+G+GKFS E+E +LNLKNPP+KNTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 484 WHLHGHDFWVLGYGDGKFSAEEESSLNLKNPPLKNTVVIFPYGWTAIRFVADNPGVWAFH 543

Query: 541 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLTGQ 567
           CHIEPHLHMGMG++FAEGVD+VG IP  AL+CG+T +
Sbjct: 544 CHIEPHLHMGMGVVFAEGVDKVGRIPTKALACGVTAK 573

[Close]
BLAST of Tan0013838 vs. NCBI nr
Match: KAA0055636.1 (L-ascorbate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 823.9 bits (2127), Expect = 8.3e-235
Identity = 391/578 (67.65%), Postives = 458/578 (79.24%), Query Frame = 0 

Query: 6   EPFW-RDFRVFFIIFYASIFKFGLSESS----QTRKFQWDVEYMFWSPDCKENMVMGING 65
           +PF+ + F VFF++  + IF FG++ S     + R ++W+VEYMFWSPDC EN+VMGING
Sbjct: 7   KPFFPKPFLVFFVL--SIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGING 66

Query: 66  QFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETF 125
           QFPGPTI+ANAGD VVV +TNKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGETF
Sbjct: 67  QFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETF 126

Query: 126 TYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGV 185
           TY  +VDKAGTYFYHGH GMQR AGLYGSLIVD  EG  EPFHYDEE+NLLLSDWWHQ V
Sbjct: 127 TYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSV 186

Query: 186 HELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILK 245
           H+ EVGLSS P RWIG+PQS+LINGRGQF CS+AAKY   + +KQCEL G EQC+PFIL 
Sbjct: 187 HKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKY--KNGLKQCELSGKEQCAPFILH 246

Query: 246 VEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIA 305
           V P KTYR+RIASTT+LASLN AIGNH ++VVE DGNYV+PF+T  IDI+SGESYSVLI 
Sbjct: 247 VHPKKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLIT 306

Query: 306 TNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNFA 365
           T+Q+PS+NYW+S  V  + P TPPGLT+LNYLP   + LPISP    P W+D  R KNF 
Sbjct: 307 TDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFT 366

Query: 366 KQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQA 425
            +I A   SPKPP  + RR+FLLNTQN+ING  KWA+NNVSL LP TPYL A+KF L+ A
Sbjct: 367 FRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTA 426

Query: 426 FDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPW 485
           F+Q+ PPETFP  Y+I+ PP N  T   N +Y+  +G TVD +LQN NML+ N SE HPW
Sbjct: 427 FNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPW 486

Query: 486 HL------FLGFGEGKFS-EEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 545
           HL       LG+GEGKFS  ED + LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 487 HLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 546

Query: 546 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLTGQM 568
           CHIEPHLHMGMG+IFAEGV +VG IP NA++CG T  +
Sbjct: 547 CHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTAMI 580

[Close]
BLAST of Tan0013838 vs. NCBI nr
Match: XP_038878978.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 823.5 bits (2126), Expect = 1.1e-234
Identity = 390/570 (68.42%), Postives = 455/570 (79.82%), Query Frame = 0 

Query: 8   FWRDFRVFFIIFYASIFKFGLSESS----QTRKFQWDVEYMFWSPDCKENMVMGINGQFP 67
           F + F +FFI+  + IF FG++ S     + R ++W+VEYMFWSPDC EN+VMGI+GQFP
Sbjct: 10  FPKPFLIFFIL--SIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGIDGQFP 69

Query: 68  GPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFTYT 127
           GPTI+AN GDTVVV +TNKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGETFTY 
Sbjct: 70  GPTIRANVGDTVVVELTNKLHTEGVVIHWHGIMQRGTPWADGTASISQCAINPGETFTYR 129

Query: 128 LIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGVHEL 187
            +VDKAGTYFYHGH GMQR AGLYGSLIVD  EG+ EPFHYDEE+NLLLSDWWHQ VH+ 
Sbjct: 130 FVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDWWHQSVHKQ 189

Query: 188 EVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILKVEP 247
           EVGLSS P RWIG+PQ++LINGRGQF CS+AAKY  ++++KQCELKG EQC+PFIL V P
Sbjct: 190 EVGLSSKPIRWIGEPQTILINGRGQFDCSIAAKY--NNNLKQCELKGGEQCAPFILHVLP 249

Query: 248 NKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIATNQ 307
           NKTYR+RIASTT+LASLN AIGNH ++VVE DGNY++PF T  IDI+SGESYSVLI T+Q
Sbjct: 250 NKTYRIRIASTTALASLNFAIGNHRLLVVEADGNYIQPFLTSDIDIYSGESYSVLITTDQ 309

Query: 308 DPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNFAKQI 367
             S+NYW+S  V  + P TPPGLTILNYLP   + LPISP    P W+D  R KNF  +I
Sbjct: 310 PSSENYWVSIGVRARLPKTPPGLTILNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRI 369

Query: 368 TATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQAFDQ 427
            A   SPKPP  + RRIFLLNTQN++NG  KWA+NNVSL LP TPYL A+KF LN AFDQ
Sbjct: 370 FAAMGSPKPPMRYNRRIFLLNTQNRMNGFVKWAINNVSLALPPTPYLAAMKFKLNTAFDQ 429

Query: 428 SNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPWHL- 487
           + PPETFP  Y+I+ PP N+ T   N +YR  +G  VD +LQN NML++N SE HPWHL 
Sbjct: 430 NPPPETFPIDYDINNPPQNANTITGNGVYRFNMGEIVDVILQNANMLNDNTSEIHPWHLH 489

Query: 488 -----FLGFGEGKFSEE-DEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFHCHI 547
                 LG+GEGKFS   DEK LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FHCHI
Sbjct: 490 GHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHI 549

Query: 548 EPHLHMGMGIIFAEGVDRVGDIPKNALSCG 563
           EPHLHMGMG++FAEGV +VG IP  AL+CG
Sbjct: 550 EPHLHMGMGVVFAEGVHKVGRIPTKALACG 575

[Close]
BLAST of Tan0013838 vs. NCBI nr
Match: XP_023531516.1 (L-ascorbate oxidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 823.5 bits (2126), Expect = 1.1e-234
Identity = 383/577 (66.38%), Postives = 455/577 (78.86%), Query Frame = 0 

Query: 1   MGKPMEPFWRDFRVFFIIFYASIFKFGL-SESSQTRKFQWDVEYMFWSPDCKENMVMGIN 60
           MGK  EP   +F V F  F+  I  FG+ SE SQ R ++W+VEYMFW+PDC EN+VMGIN
Sbjct: 4   MGKAREP---NFLVLF--FFGLILGFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63

Query: 61  GQFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGET 120
           GQFPGPTI+ANAGD+VVV +TNKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGET
Sbjct: 64  GQFPGPTIRANAGDSVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 121 FTYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQG 180
           F Y   VD  GT+FYHGH GMQR AGLYGSLIVD  +G+ EPFHYD E+NLLLSDWWHQ 
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183

Query: 181 VHELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFIL 240
           +H+ EVGLSS P RWIG+PQ++L+NGRGQF CS+AAKYD  S+++ C+LKG+E C+P+I 
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYD--SNLEPCKLKGSESCAPYIF 243

Query: 241 KVEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLI 300
            V P KTYR+RIASTT+LA+LN AIGNH ++VVE DGNYV+PF T  IDI+SGESYSVLI
Sbjct: 244 HVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLI 303

Query: 301 ATNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNF 360
            T+Q+PS+NYW+S     + PNTPPGLT+LNYLP   + LP SP    P WDD  R KNF
Sbjct: 304 TTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNF 363

Query: 361 AKQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQ 420
             +ITA   SPKPP  F RRIFLLNTQN ING  KWA+N+VSL LP TPYLGA+K+ L  
Sbjct: 364 TYRITAAMGSPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLH 423

Query: 421 AFDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHP 480
           AFDQ+ PPE FPE Y+ID PP N  T + N +Y+ ++G  VD +LQN NM+ EN SE HP
Sbjct: 424 AFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHP 483

Query: 481 WHL------FLGFGEGKFSEEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 540
           WHL       LG+G+GKFS E+E +LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 484 WHLHGHDFWVLGYGDGKFSAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 543

Query: 541 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLTGQ 567
           CHIEPHLHMGMG++FAEGV++VG IP  AL+CG T +
Sbjct: 544 CHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAK 573

[Close]
BLAST of Tan0013838 vs. ExPASy TrEMBL
Match: A0A6J1ENQ7 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111436167 PE=3 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 2.1e-236
Identity = 385/577 (66.72%), Postives = 456/577 (79.03%), Query Frame = 0 

Query: 1   MGKPMEPFWRDFRVFFIIFYASIFKFGL-SESSQTRKFQWDVEYMFWSPDCKENMVMGIN 60
           MGK  EP   +F V F  F+  I  FG+ SE SQ R ++W+VEYMFW+PDC EN+VMGIN
Sbjct: 4   MGKAREP---NFLVLF--FFGLILGFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63

Query: 61  GQFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGET 120
           GQFPGPTI+ANAGDTVVV +TNKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGET
Sbjct: 64  GQFPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 121 FTYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQG 180
           F Y   VD  GT+FYHGH GMQR AGLYGSLIVD  +G+ EPFHYD E+NLLLSDWWHQ 
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183

Query: 181 VHELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFIL 240
           +H+ EVGLSS P RWIG+PQ++L+NGRGQF CS+AAKYD  S+++ C+LKG+E C+P+I 
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYD--SNLEPCKLKGSEPCAPYIF 243

Query: 241 KVEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLI 300
            V P KTYR+RIASTT+LA+LN AIGNH ++VVE DGNYV+PF T  IDI+SGESYSVLI
Sbjct: 244 HVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLI 303

Query: 301 ATNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNF 360
            T+Q+PS+NYW+S     + PNTPPGLT+LNYLP   + LP SP    P WDD  R KNF
Sbjct: 304 TTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNF 363

Query: 361 AKQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQ 420
             +ITA   SPKPP  F RRIFLLNTQN ING  KWA+N+VSL LP TPYLGA+K+ L  
Sbjct: 364 TYRITAAMGSPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLH 423

Query: 421 AFDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHP 480
           AFDQ+ PPE FPE Y+ID PP N  T + N +Y+ ++G  VD +LQN NM+ EN SE HP
Sbjct: 424 AFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHP 483

Query: 481 WHL------FLGFGEGKFSEEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 540
           WHL       LG+G+GKFS E+E +LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 484 WHLHGHDFWVLGYGDGKFSAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 543

Query: 541 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLTGQ 567
           CHIEPHLHMGMG++FAEGVD+VG IP  AL+CG+T +
Sbjct: 544 CHIEPHLHMGMGVVFAEGVDKVGRIPTKALACGVTAK 573

[Close]
BLAST of Tan0013838 vs. ExPASy TrEMBL
Match: A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 4.0e-235
Identity = 391/578 (67.65%), Postives = 458/578 (79.24%), Query Frame = 0 

Query: 6   EPFW-RDFRVFFIIFYASIFKFGLSESS----QTRKFQWDVEYMFWSPDCKENMVMGING 65
           +PF+ + F VFF++  + IF FG++ S     + R ++W+VEYMFWSPDC EN+VMGING
Sbjct: 7   KPFFPKPFLVFFVL--SIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGING 66

Query: 66  QFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETF 125
           QFPGPTI+ANAGD VVV +TNKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGETF
Sbjct: 67  QFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETF 126

Query: 126 TYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGV 185
           TY  +VDKAGTYFYHGH GMQR AGLYGSLIVD  EG  EPFHYDEE+NLLLSDWWHQ V
Sbjct: 127 TYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSV 186

Query: 186 HELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILK 245
           H+ EVGLSS P RWIG+PQS+LINGRGQF CS+AAKY   + +KQCEL G EQC+PFIL 
Sbjct: 187 HKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKY--KNGLKQCELSGKEQCAPFILH 246

Query: 246 VEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIA 305
           V P KTYR+RIASTT+LASLN AIGNH ++VVE DGNYV+PF+T  IDI+SGESYSVLI 
Sbjct: 247 VHPKKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLIT 306

Query: 306 TNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNFA 365
           T+Q+PS+NYW+S  V  + P TPPGLT+LNYLP   + LPISP    P W+D  R KNF 
Sbjct: 307 TDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFT 366

Query: 366 KQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQA 425
            +I A   SPKPP  + RR+FLLNTQN+ING  KWA+NNVSL LP TPYL A+KF L+ A
Sbjct: 367 FRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTA 426

Query: 426 FDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPW 485
           F+Q+ PPETFP  Y+I+ PP N  T   N +Y+  +G TVD +LQN NML+ N SE HPW
Sbjct: 427 FNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPW 486

Query: 486 HL------FLGFGEGKFS-EEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 545
           HL       LG+GEGKFS  ED + LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 487 HLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 546

Query: 546 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLTGQM 568
           CHIEPHLHMGMG+IFAEGV +VG IP NA++CG T  +
Sbjct: 547 CHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTAMI 580

[Close]
BLAST of Tan0013838 vs. ExPASy TrEMBL
Match: Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 2.0e-234
Identity = 390/578 (67.47%), Postives = 457/578 (79.07%), Query Frame = 0 

Query: 6   EPFW-RDFRVFFIIFYASIFKFGLSESS----QTRKFQWDVEYMFWSPDCKENMVMGING 65
           +PF+ + F VFF++  + IF FG++ S     + R ++W+VEYMFWSPDC EN+VMGING
Sbjct: 7   KPFFPKPFLVFFVL--SIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGING 66

Query: 66  QFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETF 125
           QFPGPTI+ANAGD VVV +TNKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGETF
Sbjct: 67  QFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETF 126

Query: 126 TYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGV 185
           TY  +VDKAGTYFYHGH GMQR AGLYGSLIVD  EG  EPFHYDEE+NLLLSDWWHQ V
Sbjct: 127 TYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSV 186

Query: 186 HELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILK 245
           H+ EVGLSS P RWIG+PQS+LINGRGQF CS+AAKY   + +KQCEL G EQC+PFIL 
Sbjct: 187 HKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKY--KNGLKQCELSGKEQCAPFILH 246

Query: 246 VEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIA 305
           V P K YR+RIASTT+LASLN AIGNH ++VVE DGNYV+PF+T  IDI+SGESYSVLI 
Sbjct: 247 VHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLIT 306

Query: 306 TNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNFA 365
           T+Q+PS+NYW+S  V  + P TPPGLT+LNYLP   + LPISP    P W+D  R KNF 
Sbjct: 307 TDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFT 366

Query: 366 KQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQA 425
            +I A   SPKPP  + RR+FLLNTQN+ING  KWA+NNVSL LP TPYL A+KF L+ A
Sbjct: 367 FRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTA 426

Query: 426 FDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPW 485
           F+Q+ PPETFP  Y+I+ PP N  T   N +Y+  +G TVD +LQN NML+ N SE HPW
Sbjct: 427 FNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPW 486

Query: 486 HL------FLGFGEGKFS-EEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 545
           HL       LG+GEGKFS  ED + LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 487 HLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 546

Query: 546 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLTGQM 568
           CHIEPHLHMGMG+IFAEGV +VG IP NA++CG T  +
Sbjct: 547 CHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTAMI 580

[Close]
BLAST of Tan0013838 vs. ExPASy TrEMBL
Match: A0A6J1HPZ9 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466657 PE=3 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 1.7e-233
Identity = 382/577 (66.20%), Postives = 454/577 (78.68%), Query Frame = 0 

Query: 1   MGKPMEPFWRDFRVFFIIFYASIFKFGL-SESSQTRKFQWDVEYMFWSPDCKENMVMGIN 60
           MGK  EP   +F + F  F+  I  FG+ SE SQ R ++W+VEYMFW+PDC EN+VMGIN
Sbjct: 4   MGKAREP---NFLILF--FFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63

Query: 61  GQFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGET 120
           GQFPGPTI+ANAGDTVVV + NKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGET
Sbjct: 64  GQFPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123

Query: 121 FTYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQG 180
           F Y   VD  GT+FYHGH GMQR AGLYGSLIVD  +G+ EPFHYD E+NLLLSDWWHQ 
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183

Query: 181 VHELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFIL 240
           +H+ EVGLSS P RWIG+PQ++L+NGRGQF CS+AAKYD  S+++ C+LKG+E C+P+I 
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYD--SNLEPCKLKGSEPCAPYIF 243

Query: 241 KVEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLI 300
            V P KTYR+RIASTT+LA+LN AIGNH ++VVE DGNYV+PF T  IDI+SGESYSVLI
Sbjct: 244 HVMPKKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLI 303

Query: 301 ATNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNF 360
            T+Q+PS+NYW+S    G+ PNTPPGLT+LNYLP   + LP SP    P WDD  R KNF
Sbjct: 304 TTDQNPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNF 363

Query: 361 AKQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQ 420
             +ITA   SPKPP    RRIFLLNTQN ING  KWA+N+VSL LP TPYLGA+KF L  
Sbjct: 364 TYRITAAMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLH 423

Query: 421 AFDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHP 480
           AFDQ+ PPE FPE Y+ID PP N  T + N +Y+ ++G  VD +LQN NM+ EN SE HP
Sbjct: 424 AFDQNPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHP 483

Query: 481 WHL------FLGFGEGKFSEEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 540
           WHL       LG+G+GKF+ E+E +LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 484 WHLHGHDFWVLGYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 543

Query: 541 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLTGQ 567
           CHIEPHLHMGMG++FAEGV++VG IP  AL+CG T +
Sbjct: 544 CHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAK 573

[Close]
BLAST of Tan0013838 vs. ExPASy TrEMBL
Match: E7BBM8 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.9e-233
Identity = 388/575 (67.48%), Postives = 454/575 (78.96%), Query Frame = 0 

Query: 6   EPFW-RDFRVFFIIFYASIFKFGLSESS----QTRKFQWDVEYMFWSPDCKENMVMGING 65
           +PF+ + F  F ++  + IF FG++ S     + + ++WDVEYMFWSPDC EN+VMGING
Sbjct: 7   KPFFPKPFLSFLVL--SIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGING 66

Query: 66  QFPGPTIKANAGDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETF 125
           +FPGPTI+ANAGD VVV +TNKL TEGVVIHWHGI Q GTPWADGTA+ISQCAINPGETF
Sbjct: 67  EFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETF 126

Query: 126 TYTLIVDKAGTYFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGV 185
           TY  +VDKAGTYFYHGH GMQR AGLYGSLIVD  EG  EPFHYDEE+NLLLSDWWHQ V
Sbjct: 127 TYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSV 186

Query: 186 HELEVGLSSSPTRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILK 245
           H+ EVGLSS P RWIG+PQS+LING+GQF CS+AAKY  +  +KQCEL G E+C+PFIL 
Sbjct: 187 HKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKY--NQGLKQCELSGKEKCAPFILH 246

Query: 246 VEPNKTYRLRIASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIA 305
           V+P KTYR+RIASTT+LASLN AIGNH ++VVE DGNYV+PF T  IDI+SGESYSVLI 
Sbjct: 247 VQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLIT 306

Query: 306 TNQDPSKNYWISTAVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNFA 365
           T+Q+P +NYW+S  V  + P TPPGLT+LNYLP  A+ LPISP    P W+D  R KNF 
Sbjct: 307 TDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFT 366

Query: 366 KQITATATSPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQA 425
            +I A   SPKPP  + RR+FLLNTQN+ING  KWA+NNVSL LP TPYL A+K  LN A
Sbjct: 367 FRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTA 426

Query: 426 FDQSNPPETFPEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPW 485
           F+QS PPETFP  Y+I+ PP N  TT  N +Y+  +G TVD +LQN NML+ N SE HPW
Sbjct: 427 FNQSPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPW 486

Query: 486 HL------FLGFGEGKF-SEEDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFH 545
           HL       LG+GEGKF + EDEK LNLKNPP++NTVVIFP+GWTA+RFVADNPGVW FH
Sbjct: 487 HLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFH 546

Query: 546 CHIEPHLHMGMGIIFAEGVDRVGDIPKNALSCGLT 565
           CHIEPHLHMGMG++FAEGV  VG IP  AL+CG T
Sbjct: 547 CHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGST 577

[Close]
BLAST of Tan0013838 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 728.8 bits (1880), Expect = 3.4e-210
Identity = 333/564 (59.04%), Postives = 424/564 (75.18%), Query Frame = 0 

Query: 14  VFFIIFYASIFKFGLSESSQTRKFQWDVEYMFWSPDCKENMVMGINGQFPGPTIKANAGD 73
           V++I+   ++     + S+  R++ W+VEY +WSPDCKE  VM +NG+FPGPTIKA AGD
Sbjct: 19  VWWIVLVVAVLTH--TASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGD 78

Query: 74  TVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFTYTLIVDKAGTYF 133
           T+VVN+TNKL TEG+VIHWHGIRQ G+PWADG A ++QCAINPGETFTY   V+K GT+F
Sbjct: 79  TIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHF 138

Query: 134 YHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGVHELEVGLSSSPTR 193
           YHGHYGMQR AGLYGSLIVDVA+G+ E   YD E NLLLSDWWH+ +   E+GLSS P R
Sbjct: 139 YHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMR 198

Query: 194 WIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILKVEPNKTYRLRIAS 253
           WIG+ QS+LINGRGQF CSLAA++ +++S+  C  K  +QC+P IL VEPNKTYR+R++S
Sbjct: 199 WIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSS 258

Query: 254 TTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIATNQDPSKNYWIST 313
           TT+LASLN+A+  H ++VVE DGNY+ PFTT  IDI+SGESYSVL+ T+QDPS+NY+IS 
Sbjct: 259 TTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISV 318

Query: 314 AVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNFAKQITATATSPKPP 373
            V G++PNT   LTILNY+  PA+ LP SP    P+WDD  R KNF+K+I +   SP PP
Sbjct: 319 GVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPP 378

Query: 374 QNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQAFDQSNPPETFPEG 433
           + + +R+ LLNTQN I+G  KWA+NNVSL  P TPYLG++K+ L   F++ +PP ++   
Sbjct: 379 KKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMD 438

Query: 434 YNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPWHL------FLGFG 493
           Y+I  PP    TT  N IY      TVD ++QN N+L    SE HPWHL       LG+G
Sbjct: 439 YDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYG 498

Query: 494 EGKFSEE-DEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFHCHIEPHLHMGMGI 553
           +GKF    DEK  NLKNPP++NT +++P+GWTA+RFV DNPGVW FHCHIEPHLHMGMG+
Sbjct: 499 DGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGV 558

Query: 554 IFAEGVDRVGDIPKNALSCGLTGQ 567
           +FAEG++R+G +P  AL CGLT Q
Sbjct: 559 VFAEGLNRIGKVPDEALGCGLTKQ 580

[Close]
BLAST of Tan0013838 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 672.5 bits (1734), Expect = 2.9e-193
Identity = 321/550 (58.36%), Postives = 392/550 (71.27%), Query Frame = 0 

Query: 29  SESSQTRKFQWDVEYMFWSPDCKENMVMGINGQFPGPTIKANAGDTVVVNVTNKLFTEGV 88
           S S+   +  W+VEY +W PDCKE +VM INGQFPGPTI A AGDTV+++V NKL TEGV
Sbjct: 18  SASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGV 77

Query: 89  VIHWHGIRQIGTPWADGTAAISQCAINPGETFTYTLIVDKAGTYFYHGHYGMQRMAGLYG 148
           VIHWHGIRQ GTPWADG A ++QC INPGETFTY  IVDKAGT+FYHGHYGMQR +GLYG
Sbjct: 78  VIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYG 137

Query: 149 SLIVDVAEGEIEPFHYDEELNLLLSDWWHQGVHELEVGLSSSPTRWIGDPQSLLINGRGQ 208
            LIV       E   YD E NLLLSDWWHQ +H  E+ LSS P RWIG+PQSLLINGRGQ
Sbjct: 138 MLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELALSSRPMRWIGEPQSLLINGRGQ 197

Query: 209 FGCSLAAKYDSSSSMKQCELKGNEQCSPFILKVEPNKTYRLRIASTTSLASLNVAIGNHN 268
           F CS AA ++       C  K N+QC+P  L+VEPN+ YRLRIASTT+LASLN+A+  H 
Sbjct: 198 FNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQGHQ 257

Query: 269 MIVVECDGNYVEPFTTPSIDIHSGESYSVLIATNQDPSKNYWISTAVTGQQPNTPPGLTI 328
           ++VVE DGNYV PFT   ID++SGE+YSVL+ TN  PSK YWIS  V G++P TP  LT+
Sbjct: 258 LVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQALTV 317

Query: 329 LNYLPT----PATDLPISPPQWDDMTRLKNFAKQITATATSPKPPQNFTRRIFLLNTQNK 388
           +NY+      P+   P++ P W+D  R K+F+K+I A    PKPP+    ++ LLNTQN 
Sbjct: 318 INYVDATESRPSHPPPVT-PIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQLILLNTQNL 377

Query: 389 INGVEKWALNNVSLTLPTTPYLGAIKFWLNQAFDQSNP-PETFPEGYNIDVPPVNSATTV 448
                KW++NNVSL++P TPYLG+I++ L  A+D  +P  +   + Y+I  PP N  TT 
Sbjct: 378 YEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPNTTK 437

Query: 449 NNAIYRLELGSTVDFVLQNTNMLHENESENHPWHL------FLGFGEGKFSEE-DEKNLN 508
            + IY    G  VD +LQN N+L    SE HPWH+       LG+GEGKF    DEK  N
Sbjct: 438 GSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEKTFN 497

Query: 509 LKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFHCHIEPHLHMGMGIIFAEGVDRVG--DI 565
           LKNPP++NTVV++PFGWTA+RFV DNPGVW FHCHIEPHLHMGMG++F EGVDR+G  +I
Sbjct: 498 LKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGKMEI 557

[Close]
BLAST of Tan0013838 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 654.1 bits (1686), Expect = 1.1e-187
Identity = 303/522 (58.05%), Postives = 388/522 (74.33%), Query Frame = 0 

Query: 14  VFFIIFYASIFKFGLSESSQTRKFQWDVEYMFWSPDCKENMVMGINGQFPGPTIKANAGD 73
           V++I+   ++     + S+  R++ W+VEY +WSPDCKE  VM +NG+FPGPTIKA AGD
Sbjct: 19  VWWIVLVVAVLTH--TASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGD 78

Query: 74  TVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFTYTLIVDKAGTYF 133
           T+VVN+TNKL TEG+VIHWHGIRQ G+PWADG A ++QCAINPGETFTY   V+K GT+F
Sbjct: 79  TIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHF 138

Query: 134 YHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGVHELEVGLSSSPTR 193
           YHGHYGMQR AGLYGSLIVDVA+G+ E   YD E NLLLSDWWH+ +   E+GLSS P R
Sbjct: 139 YHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMR 198

Query: 194 WIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILKVEPNKTYRLRIAS 253
           WIG+ QS+LINGRGQF CSLAA++ +++S+  C  K  +QC+P IL VEPNKTYR+R++S
Sbjct: 199 WIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSS 258

Query: 254 TTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIATNQDPSKNYWIST 313
           TT+LASLN+A+  H ++VVE DGNY+ PFTT  IDI+SGESYSVL+ T+QDPS+NY+IS 
Sbjct: 259 TTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISV 318

Query: 314 AVTGQQPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNFAKQITATATSPKPP 373
            V G++PNT   LTILNY+  PA+ LP SP    P+WDD  R KNF+K+I +   SP PP
Sbjct: 319 GVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPP 378

Query: 374 QNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQAFDQSNPPETFPEG 433
           + + +R+ LLNTQN I+G  KWA+NNVSL  P TPYLG++K+ L   F++ +PP ++   
Sbjct: 379 KKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMD 438

Query: 434 YNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPWHL------FLGFG 493
           Y+I  PP    TT  N IY      TVD ++QN N+L    SE HPWHL       LG+G
Sbjct: 439 YDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYG 498

Query: 494 EGKFSEE-DEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPG 525
           +GKF    DEK  NLKNPP++NT +++P+GWTA+RFV DNPG
Sbjct: 499 DGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]
BLAST of Tan0013838 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 552.7 bits (1423), Expect = 3.3e-157
Identity = 273/569 (47.98%), Postives = 360/569 (63.27%), Query Frame = 0 

Query: 12  FRVFFIIFYASIFKFGLSESSQTRKFQWDVEYMFWSPDCKENMVMGINGQFPGPTIKANA 71
           F +  + F A  F   L +  + R+F+W+V+Y F SPDC E +V+ ING+FPGPTIKA  
Sbjct: 14  FNLMVLCFIALFFSSVLCQ-GKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQ 73

Query: 72  GDTVVVNVTNKLFTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFTYTLIVDKAGT 131
           GDT+VV + N   TE V +HWHGIRQIGTPW DG   ++QC I PGE F Y  +VD+ GT
Sbjct: 74  GDTIVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGT 133

Query: 132 YFYHGHYGMQRMAGLYGSLIVDVAEGEIEPFHYDEELNLLLSDWWHQGVHELEVGLSSSP 191
           Y YH HYGMQR +GL G + V     E EPF YD + N LL+DW+H+ + E   GL+S P
Sbjct: 134 YMYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIP 193

Query: 192 TRWIGDPQSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILKVEPNKTYRLRI 251
            +W+G+PQSL+I GRG+F CS       S     C +  N  CS FIL V P KTYRLRI
Sbjct: 194 FKWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNV-SNADCSRFILTVIPGKTYRLRI 253

Query: 252 ASTTSLASLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIATNQDPSKNYWI 311
            S T+L++L+  I  HN+ VVE DG+YVEPFT  ++ ++SGE+YSVL+  +Q+P +NYWI
Sbjct: 254 GSLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWI 313

Query: 312 STAVTGQQPNTPPGLTILNYLPT------PATDLPISPPQWDD-MTRLKNFAKQITATAT 371
           ++++  +   TPP   +LNY P       P ++     P+W+D  +RL            
Sbjct: 314 TSSIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGF 373

Query: 372 SPKPPQNFTRRIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQAFD-QSNPP 431
               P+N  + I LLNTQN++NG  +W++NNVS   P TPYL A+K  L  AFD +   P
Sbjct: 374 IHALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAP 433

Query: 432 ETF-PEGYNIDVPPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPWHL---- 491
           E +    Y+I   P+N+  T ++ IYRL   STVD +LQN N ++ N SE HPWHL    
Sbjct: 434 ENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHD 493

Query: 492 --FLGFGEGKFSE-EDEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFHCHIEPH 551
              LG+GEGKF+E ED K  N  +P  KNTV + PFGWTA+RF ADNPGVW FHCHIE H
Sbjct: 494 FWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESH 553

Query: 552 LHMGMGIIFAEGVDRVGDIPKNALSCGLT 565
             MGMGI+F  G+D+V  +P + + CG T
Sbjct: 554 FFMGMGIVFESGIDKVSSLPSSIMGCGQT 580

[Close]
BLAST of Tan0013838 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 459.9 bits (1182), Expect = 2.9e-129
Identity = 213/379 (56.20%), Postives = 278/379 (73.35%), Query Frame = 0 

Query: 199 QSLLINGRGQFGCSLAAKYDSSSSMKQCELKGNEQCSPFILKVEPNKTYRLRIASTTSLA 258
           +S+LINGRGQF CSLAA++ +++S+  C  K  +QC+P IL VEPNKTYR+R++STT+LA
Sbjct: 11  KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70

Query: 259 SLNVAIGNHNMIVVECDGNYVEPFTTPSIDIHSGESYSVLIATNQDPSKNYWISTAVTGQ 318
           SLN+A+  H ++VVE DGNY+ PFTT  IDI+SGESYSVL+ T+QDPS+NY+IS  V G+
Sbjct: 71  SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130

Query: 319 QPNTPPGLTILNYLPTPATDLPISP----PQWDDMTRLKNFAKQITATATSPKPPQNFTR 378
           +PNT   LTILNY+  PA+ LP SP    P+WDD  R KNF+K+I +   SP PP+ + +
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRK 190

Query: 379 RIFLLNTQNKINGVEKWALNNVSLTLPTTPYLGAIKFWLNQAFDQSNPPETFPEGYNIDV 438
           R+ LLNTQN I+G  KWA+NNVSL  P TPYLG++K+ L   F++ +PP ++   Y+I  
Sbjct: 191 RLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMN 250

Query: 439 PPVNSATTVNNAIYRLELGSTVDFVLQNTNMLHENESENHPWHL------FLGFGEGKFS 498
           PP    TT  N IY      TVD ++QN N+L    SE HPWHL       LG+G+GKF 
Sbjct: 251 PPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFK 310

Query: 499 EE-DEKNLNLKNPPVKNTVVIFPFGWTAVRFVADNPGVWIFHCHIEPHLHMGMGIIFAEG 558
              DEK  NLKNPP++NT +++P+GWTA+RFV DNPGVW FHCHIEPHLHMGMG++FAEG
Sbjct: 311 PGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEG 370

Query: 559 VDRVGDIPKNALSCGLTGQ 567
           ++R+G +P  AL CGLT Q
Sbjct: 371 LNRIGKVPDEALGCGLTKQ 389

The following BLAST results are available for this feature:
BLAST of Tan0013838 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P247924.6e-23666.20L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2[more]
P141331.7e-23567.30L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1[more]
P370644.7e-23367.52L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1[more]
Q405884.6e-22064.20L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1[more]
M4DUF24.0e-20861.06L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1[more]
BLAST of Tan0013838 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022929646.14.4e-23666.72L-ascorbate oxidase [Cucurbita moschata][more]
KAG6588158.17.5e-23666.72L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022051... [more]
KAA0055636.18.3e-23567.65L-ascorbate oxidase [Cucumis melo var. makuwa][more]
XP_038878978.11.1e-23468.42L-ascorbate oxidase [Benincasa hispida][more]
XP_023531516.11.1e-23466.38L-ascorbate oxidase [Cucurbita pepo subsp. pepo][more]
BLAST of Tan0013838 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1ENQ72.1e-23666.72L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111436167 PE=3 SV=1[more]
A0A5A7UMY34.0e-23567.65L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G... [more]
Q9M5B42.0e-23467.47L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1[more]
A0A6J1HPZ91.7e-23366.20L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466657 PE=3 SV=1[more]
E7BBM82.9e-23367.48L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1[more]
BLAST of Tan0013838 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G21105.13.4e-21059.04Plant L-ascorbate oxidase [more]
AT5G21100.12.9e-19358.36Plant L-ascorbate oxidase [more]
AT5G21105.31.1e-18758.05Plant L-ascorbate oxidase [more]
AT4G39830.13.3e-15747.98Cupredoxin superfamily protein [more]
AT5G21105.22.9e-12956.20Plant L-ascorbate oxidase [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 22..153
e-value: 3.5E-47
score: 162.1
IPR008972CupredoxinGENE3D2.60.40.420coord: 162..365
e-value: 9.6E-65
score: 220.4
IPR008972CupredoxinGENE3D2.60.40.420coord: 366..566
e-value: 6.1E-63
score: 214.3
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 161..359
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 368..561
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 31..178
IPR017760L-ascorbate oxidase, plantsTIGRFAMTIGR03388TIGR03388coord: 34..564
e-value: 1.9E-270
score: 896.1
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 440..548
e-value: 4.2E-26
score: 91.3
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 169..332
e-value: 1.4E-46
score: 158.5
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 41..154
e-value: 3.2E-42
score: 143.3
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 30..564
NoneNo IPR availablePANTHERPTHR11709:SF394PLANT L-ASCORBATE OXIDASEcoord: 30..564
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 524..544
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 529..540
IPR034259Ascorbate oxidase, first cupredoxin domainCDDcd13845CuRO_1_AAOcoord: 35..154
e-value: 1.69015E-79
score: 243.507

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0013838.1Tan0013838.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity