Tan0013746 (gene) Snake gourd v1

BLAST of Tan0013746 vs. ExPASy Swiss-Prot
Match: A0A3Q7JC00 (Mediator of RNA polymerase II transcription subunit 25 OS=Solanum lycopersicum OX=4081 GN=MED25 PE=1 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 8.8e-252
Identity = 546/845 (64.62%), Postives = 646/845 (76.45%), Query Frame = 0

Query: 5   QLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTHGSYC 64
           +LIV VEGTA +GP+W+ +V+DY+DKI+RCF G + + QK   ++VE S+V F+THG Y 
Sbjct: 4   KLIVAVEGTAVLGPYWKIIVSDYLDKIIRCFFGVDSTSQKSSAADVEVSMVMFNTHGPYS 63

Query: 65  GCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQQTMD 124
            CLVQR+GWT+D+D F+QWLSAIPFSGGGF+DAA+AEGL EAL+MF +  +G Q QQ M+
Sbjct: 64  ACLVQRSGWTKDMDTFLQWLSAIPFSGGGFNDAAVAEGLAEALVMF-SVPNGNQTQQKME 123

Query: 125 MQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCFISLS 184
            +KHCIL++ SNPYPLPTPVYRP +Q LEQ++N+E Q+ +  + AETVAK+F QC ISLS
Sbjct: 124 GKKHCILISGSNPYPLPTPVYRPQMQKLEQNENIEAQTDSRLADAETVAKTFPQCSISLS 183

Query: 185 VICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGITSLP 244
           VICPK+LPKLRAIY AGK NPRAADPPID AK P++LVLISENF+EARAA SR G+T+L 
Sbjct: 184 VICPKKLPKLRAIYDAGKHNPRAADPPIDTAKNPNFLVLISENFIEARAAFSRSGLTNLA 243

Query: 245 ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTSMTNG 304
           +N SPVKMD+S V+PV+G    +  +AN  +I+R P+S  N+PPATVK+EPNTVT MT G
Sbjct: 244 SNHSPVKMDVSSVLPVSGTQSISNSAANVSVISRPPISAGNIPPATVKIEPNTVTPMT-G 303

Query: 305 SAYPPHMPSVVRAASQGVPSLQTSSPLS-SQEMITNNENTQDLKPLVNGIPQPLRPV--G 364
             +  H+PS VR A Q VPSLQ SSPLS SQEM+++ EN Q++KP+V+G+ Q LRPV   
Sbjct: 304 PGF-SHIPS-VRPALQPVPSLQASSPLSVSQEMVSHTENVQEMKPIVSGMTQSLRPVAAA 363

Query: 365 PANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNVY 424
            ANV ILN ++QA       L GGTSIGL SMG TPM   +S+MISSGM SSVPA+Q V 
Sbjct: 364 AANVKILNGVAQAH----QVLGGGTSIGLQSMGGTPM---LSSMISSGMASSVPASQAVL 423

Query: 425 SSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQSV 484
           SSGQSG+T++ G+  L   A N+  S+ SS ++  P  S                  Q+V
Sbjct: 424 SSGQSGVTTMTGAVPLAGSAQNTQNSAPSSFTSTAPSMS-----------------GQTV 483

Query: 485 PGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGVS 544
           P MSQGN+ GTQ++ SG GMNQN++  L   G+ SG GTM+PTPGM QQ Q GM  +GV 
Sbjct: 484 PAMSQGNIPGTQMMPSGTGMNQNMLTGLGATGLPSGTGTMMPTPGMSQQGQPGMQPVGV- 543

Query: 545 NNAAANMPLS-QHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 604
           N+ +ANMPLS Q T+GAL  AQSKYVK WEGNLSGQRQGQPVFITR+EGYRSASAS+ LA
Sbjct: 544 NSTSANMPLSQQQTSGALPSAQSKYVKVWEGNLSGQRQGQPVFITRLEGYRSASASESLA 603

Query: 605 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 664
           ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFL QLQEKKLCAVIQLPSQTL
Sbjct: 604 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLSQLQEKKLCAVIQLPSQTL 663

Query: 665 LLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQMQQQQQQQMQQQQQQQMQQQQMQQ 724
           LLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQ QQ Q Q  Q QQQQQ QQQ +  
Sbjct: 664 LLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQQQQLQAQHPQLQQQQQQQQQHL-T 723

Query: 725 QLQQHPQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQQQQQQQQLPQLQQQQ--QQ 784
           QLQQ P       L QLQQ       QQQQQ L Q+QQQQQ   QQ Q+PQ+QQQQ  Q 
Sbjct: 724 QLQQQP-------LQQLQQ-------QQQQQPLMQLQQQQQIPLQQSQVPQMQQQQIHQM 783

Query: 785 QQQPQLSQLQTQQQIPQMQQQQQQ--MVGGGISQSYVQGPGRSQLVSQGQVSSQGQSNMP 842
           QQQ Q+ Q+Q QQQIPQMQQQQQQ  MVG G++Q+Y+QGP RSQL+SQ Q SSQG    P
Sbjct: 784 QQQQQIPQMQQQQQIPQMQQQQQQQPMVGTGMNQTYMQGPARSQLMSQSQGSSQGLPITP 804

BLAST of Tan0013746 vs. ExPASy Swiss-Prot
Match: Q7XYY2 (Mediator of RNA polymerase II transcription subunit 25 OS=Arabidopsis thaliana OX=3702 GN=MED25 PE=1 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 7.5e-235
Identity = 530/861 (61.56%), Postives = 640/861 (74.33%), Query Frame = 0

Query: 4   KQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTHGSY 63
           KQLIVV EGTAA+GP+WQT+V+DY++KI+R FCG+EL+G++ P S VE SLV F++HGSY
Sbjct: 6   KQLIVVAEGTAALGPYWQTIVSDYLEKIIRSFCGSELNGERNPVSTVELSLVIFNSHGSY 65

Query: 64  CGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQQTM 123
           C CLVQR+GWTRDVDIF+ WLS+I F GGGF++ A AEGL EALMMF      GQ Q + 
Sbjct: 66  CACLVQRSGWTRDVDIFLHWLSSIQFGGGGFNEVATAEGLAEALMMF--SPPSGQAQPSN 125

Query: 124 DMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCFISL 183
           D+++HCIL+ ASNP+ LPTPVYRP +Q++E+++N + Q+ +  S AETVA  FA+C +SL
Sbjct: 126 DLKRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLSDAETVASYFAKCSVSL 185

Query: 184 SVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGITSL 243
           SV+CPKQLP +RA+Y AGK N ++AD  ID AK   YLVLISENFVEA AALS    T+L
Sbjct: 186 SVVCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSH-SATNL 245

Query: 244 PANQSPVKMD---ISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 303
           P  QSPVK+D   ++P +PVTG PP    SANGPI NRQPVS+  VP ATVKVEP+TVTS
Sbjct: 246 PQTQSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTS 305

Query: 304 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLS-SQEMITNNENTQDLKP-LVNGIPQPLR 363
           M    ++ PH+P+V R A+Q +PS+QTSS    SQ+M++N EN  D+KP +V+G+  PLR
Sbjct: 306 MAPVPSF-PHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENAPDIKPVVVSGMTPPLR 365

Query: 364 --PVGPANVSILNNISQAR-VMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVP 423
             P G ANV++LNN+SQ R VMSSA L G  S    S+GQ+ +AMH+SNMIS+GM +S+P
Sbjct: 366 TGPPGGANVNLLNNLSQVRQVMSSAALAGAAS----SVGQSAVAMHMSNMISTGMATSLP 425

Query: 424 AAQNVYSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGV 483
            +Q V+S+GQ G+TS+ GS  L            S+ +   PG +N  + Q   N+   +
Sbjct: 426 PSQTVFSTGQQGITSMAGSGALMG----------SAQTGQSPGPNNAFSPQTTSNVASNL 485

Query: 484 SVSQSVPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGM 543
            VSQ + GM+QG+ +G  ++Q GI MNQN++  L Q  V+SG G M+PTPG+ QQ Q+G+
Sbjct: 486 GVSQPMQGMNQGSHSGA-MMQGGISMNQNMMSGLGQGNVSSGTGGMMPTPGVGQQAQSGI 545

Query: 544 HSLGVSNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASA 603
             LG SN++A NM LSQ ++GA+Q +QSKYVK WEGNLSGQRQGQPV ITR+EGYRSASA
Sbjct: 546 QQLGGSNSSAPNMQLSQPSSGAMQTSQSKYVKVWEGNLSGQRQGQPVLITRLEGYRSASA 605

Query: 604 SDKLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQL 663
           SD LAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAM+QHGFLGQLQ+KKLCAVIQL
Sbjct: 606 SDSLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMSQHGFLGQLQDKKLCAVIQL 665

Query: 664 PSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQ---MQQQQQQQMQQQQQQQ 723
           PSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIP+QQQQQQQ    QQQQQQQ+QQQQQQQ
Sbjct: 666 PSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPNQQQQQQQQLHQQQQQQQQIQQQQQQQ 725

Query: 724 --MQQQQM---QQQLQQH-PQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQQQQQQ 783
             +QQQQM   QQQ QQH  Q QQQHQLSQLQ HQQ  Q QQQQQQ  Q+ Q Q   QQQ
Sbjct: 726 QHLQQQQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQQQQQQQHQLTQLQHHHQQQ 785

Query: 784 QQLPQLQQQQQQ-----QQQPQLSQLQTQQQIPQMQQQQQQMVGGGISQSYVQGPGRSQL 843
           QQ   L Q QQQ     Q Q Q S L   QQ    QQQ QQMV GG  Q++ Q PGRSQ 
Sbjct: 786 QQASPLNQMQQQTSPLNQMQQQTSPLNQMQQ----QQQPQQMVMGG--QAFAQAPGRSQ- 836

BLAST of Tan0013746 vs. NCBI nr
Match: XP_022945395.1 (mediator of RNA polymerase II transcription subunit 25-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1388.2 bits (3592), Expect = 0.0e+00
Identity = 785/846 (92.79%), Postives = 804/846 (95.04%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVTDYI+KIVRCF GNEL+GQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQ 120
           GSYCGCLVQRTGWTRDVD FIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQ+GGQNQ
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 QTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCF 180
           QTMD+QKHCILVAASNPYPLPTPVYRP V +LEQ DNVEPQSG+SQSYAETVAKSFAQCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGI 240
           ISLSVICPKQLPKLRAIY AGKRN RAADPPID  K PSYLVLISENFVEARAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 300
           TSL ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVS+ANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGIPQPLRPV 360
           MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNG+ QPLRPV
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420
           GPANVSILNNISQARV   ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

Query: 421 YSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQS 480
           +SSGQSG+TSINGS+TLTQVAPNSGLSSLSSA+NNIPGN+NIPTSQA+GNLQGGV+VSQS
Sbjct: 421 FSSGQSGLTSINGSSTLTQVAPNSGLSSLSSANNNIPGNTNIPTSQAVGNLQGGVNVSQS 480

Query: 481 VPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGV 540
           VPGMSQGNLAGTQVVQSGI MNQNVI NLSQPGV+SGNGTMIPTPGMPQQVQNGMHSLGV
Sbjct: 481 VPGMSQGNLAGTQVVQSGISMNQNVISNLSQPGVSSGNGTMIPTPGMPQQVQNGMHSLGV 540

Query: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600
           SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA
Sbjct: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600

Query: 601 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660
           ANWP  MQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL
Sbjct: 601 ANWPQIMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660

Query: 661 LLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQMQQQQQQQMQQQQQQQMQQQQMQQ 720
           LLSVSDKACRLIGMLFPGDMVVFKPQI SQ       QQQQQQQ+QQQQQQQMQQQQMQQ
Sbjct: 661 LLSVSDKACRLIGMLFPGDMVVFKPQISSQ-------QQQQQQQIQQQQQQQMQQQQMQQ 720

Query: 721 QLQQHPQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQQQQQQQQLPQLQQQQQQQQ 780
                 QLQQQHQLSQLQQHQQLPQMQQQQQQLPQM  QQQQQQQQQQLPQ+ QQQQQ Q
Sbjct: 721 ------QLQQQHQLSQLQQHQQLPQMQQQQQQLPQM--QQQQQQQQQQLPQI-QQQQQPQ 780

Query: 781 QPQLSQLQTQQQIPQM----QQQQQQMVGGGISQSYVQGPGRSQLVSQGQVSSQGQSNMP 840
           QPQLSQLQTQQQIPQM    QQQQQQMVGGGI QSYVQGPGRSQLVSQGQV+SQGQSNMP
Sbjct: 781 QPQLSQLQTQQQIPQMQQQQQQQQQQMVGGGIGQSYVQGPGRSQLVSQGQVASQGQSNMP 827

Query: 841 GGSFMG 843
           GGSFMG
Sbjct: 841 GGSFMG 827

BLAST of Tan0013746 vs. NCBI nr
Match: XP_023541647.1 (mediator of RNA polymerase II transcription subunit 25-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 793/886 (89.50%), Postives = 816/886 (92.10%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVTDYI+KIVRCF GNEL+GQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQ 120
           GSYCGCLVQRTGWTRDVD FIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQ+GGQNQ
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 QTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCF 180
           QTMD+QKHCILVAASNPYPLPTPVYRP V +LEQ DNVEPQSG+SQSYAETVAKSFAQCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPAVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGI 240
           ISLSVICPKQLPKL+AIY AGKRN RAADPPID  K PSYLVLISENFVEARAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLKAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 300
           TSL ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVS+ANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGIPQPLRPV 360
           MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNG+ QPLRPV
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420
           GPANVSILNNISQARV   ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

Query: 421 YSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQS 480
           +SSGQSG+TSINGS+TLTQVAPNSGLSSLSSA+NNIPG++NIPTSQA+GNLQGGV+VSQS
Sbjct: 421 FSSGQSGLTSINGSSTLTQVAPNSGLSSLSSANNNIPGSTNIPTSQAVGNLQGGVNVSQS 480

Query: 481 VPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGV 540
           VPGMSQGNLAGTQVVQSGI MNQNVI NLSQPGV+SGNGTMIPTPGMPQQVQNGMHSLGV
Sbjct: 481 VPGMSQGNLAGTQVVQSGISMNQNVISNLSQPGVSSGNGTMIPTPGMPQQVQNGMHSLGV 540

Query: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600
           SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA
Sbjct: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600

Query: 601 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660
           ANWP  MQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL
Sbjct: 601 ANWPQIMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660

Query: 661 LLSVSDKACRLIGMLFPGDMVVFKPQIPS-QQQQQQQMQQQQQQQMQQQQQQQMQQQQMQ 720
           LLSVSDKACRLIGMLFPGDMVVFKPQI S QQQQQQQ+QQQQQQQMQQQQQQQMQQQQMQ
Sbjct: 661 LLSVSDKACRLIGMLFPGDMVVFKPQISSQQQQQQQQIQQQQQQQMQQQQQQQMQQQQMQ 720

Query: 721 QQLQQHPQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQ---------------------- 780
           Q      QLQQQHQLSQLQQHQQLPQMQQQQQQ+PQMQ                      
Sbjct: 721 Q------QLQQQHQLSQLQQHQQLPQMQQQQQQIPQMQQQQQQQQQMQQQQQQQMQQQQQ 780

Query: 781 --------------QQQQQQQQQQQLPQLQQQQQQQ---QQPQLSQLQTQQQIPQM---- 840
                         QQQQQQQQQQQLPQ+QQQQQQQ   QQPQ+SQLQTQQQIPQM    
Sbjct: 781 QLPQMQQQQQLPQIQQQQQQQQQQQLPQIQQQQQQQQQPQQPQMSQLQTQQQIPQMQQQQ 840

Query: 841 QQQQQQMVGGGISQSYVQGPGRSQLVSQGQVSSQGQSNMPGGSFMG 843
           QQQQQQMVGGGISQSYVQGPGRSQLVSQGQV+SQGQSNMPGGSFMG
Sbjct: 841 QQQQQQMVGGGISQSYVQGPGRSQLVSQGQVASQGQSNMPGGSFMG 877

BLAST of Tan0013746 vs. NCBI nr
Match: XP_022945397.1 (mediator of RNA polymerase II transcription subunit 25-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 1379.0 bits (3568), Expect = 0.0e+00
Identity = 788/877 (89.85%), Postives = 807/877 (92.02%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVTDYI+KIVRCF GNEL+GQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQ 120
           GSYCGCLVQRTGWTRDVD FIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQ+GGQNQ
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 QTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCF 180
           QTMD+QKHCILVAASNPYPLPTPVYRP V +LEQ DNVEPQSG+SQSYAETVAKSFAQCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGI 240
           ISLSVICPKQLPKLRAIY AGKRN RAADPPID  K PSYLVLISENFVEARAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 300
           TSL ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVS+ANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGIPQPLRPV 360
           MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNG+ QPLRPV
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420
           GPANVSILNNISQARV   ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

Query: 421 YSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQS 480
           +SSGQSG+TSINGS+TLTQVAPNSGLSSLSSA+NNIPGN+NIPTSQA+GNLQGGV+VSQS
Sbjct: 421 FSSGQSGLTSINGSSTLTQVAPNSGLSSLSSANNNIPGNTNIPTSQAVGNLQGGVNVSQS 480

Query: 481 VPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGV 540
           VPGMSQGNLAGTQVVQSGI MNQNVI NLSQPGV+SGNGTMIPTPGMPQQVQNGMHSLGV
Sbjct: 481 VPGMSQGNLAGTQVVQSGISMNQNVISNLSQPGVSSGNGTMIPTPGMPQQVQNGMHSLGV 540

Query: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600
           SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA
Sbjct: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600

Query: 601 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660
           ANWP  MQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL
Sbjct: 601 ANWPQIMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660

Query: 661 LLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQMQQQQQQQMQQQQQQQMQQQQMQQ 720
           LLSVSDKACRLIGMLFPGDMVVFKPQI SQ       QQQQQQQ+QQQQQQQMQQQQMQQ
Sbjct: 661 LLSVSDKACRLIGMLFPGDMVVFKPQISSQ-------QQQQQQQIQQQQQQQMQQQQMQQ 720

Query: 721 QLQQHPQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQ------------------- 780
                 QLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQ                   
Sbjct: 721 ------QLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQQLPQMQQQQQQQQQQQQQL 780

Query: 781 -QQQQQQQLPQLQQQQQQQ-----------QQPQLSQLQTQQQIPQM----QQQQQQMVG 840
            Q QQQQQLPQ+QQQQQQQ           QQPQLSQLQTQQQIPQM    QQQQQQMVG
Sbjct: 781 PQMQQQQQLPQIQQQQQQQQLPQIQQQQQPQQPQLSQLQTQQQIPQMQQQQQQQQQQMVG 840

Query: 841 GGISQSYVQGPGRSQLVSQGQVSSQGQSNMPGGSFMG 843
           GGI QSYVQGPGRSQLVSQGQV+SQGQSNMPGGSFMG
Sbjct: 841 GGIGQSYVQGPGRSQLVSQGQVASQGQSNMPGGSFMG 861

BLAST of Tan0013746 vs. NCBI nr
Match: XP_022968268.1 (mediator of RNA polymerase II transcription subunit 25-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1377.1 bits (3563), Expect = 0.0e+00
Identity = 780/846 (92.20%), Postives = 799/846 (94.44%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVTDYI+KIVRCF GNEL+GQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQ 120
           GSYCGCLVQRTGWTRD D FIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQ+GGQNQ
Sbjct: 61  GSYCGCLVQRTGWTRDADTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 QTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCF 180
           QTMD+QKHCILVAASNPYPLPTPVYRP V +LEQ DNVEPQSG+SQSYAETVAKSFAQCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGI 240
           ISLSVICPKQLPKLRAIY AGKRN RAADPPID  K PSYLVLISENFVEARAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 300
           T+L ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVS+ANVPPATVKVEPNTVTS
Sbjct: 241 TTLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGIPQPLRPV 360
           MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNG+ QPLRPV
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420
           GPANVSILNNISQARV   ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

Query: 421 YSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQS 480
           +SSGQSG+TSINGS+TLTQVAPNSGLSSLSSA+NNIPGN+NIPTSQA+GNLQGGV+VSQS
Sbjct: 421 FSSGQSGLTSINGSSTLTQVAPNSGLSSLSSANNNIPGNTNIPTSQAVGNLQGGVNVSQS 480

Query: 481 VPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGV 540
           VPGMSQGNLAGTQVVQSGI MNQNVI NLSQPGV+SGNGTMIPTPGMPQQVQNGMHSLGV
Sbjct: 481 VPGMSQGNLAGTQVVQSGISMNQNVISNLSQPGVSSGNGTMIPTPGMPQQVQNGMHSLGV 540

Query: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600
           SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA
Sbjct: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600

Query: 601 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660
           ANWP  MQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL
Sbjct: 601 ANWPQIMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660

Query: 661 LLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQMQQQQQQQMQQQQQQQMQQQQMQQ 720
           LLSVSDKACRLIGMLFPGDMVVFKPQI SQ       QQQQQQQ+QQQQQQQMQQQQMQQ
Sbjct: 661 LLSVSDKACRLIGMLFPGDMVVFKPQISSQ-------QQQQQQQIQQQQQQQMQQQQMQQ 720

Query: 721 QLQQHPQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQQQQQQQQLPQLQQQQQQQQ 780
                 QLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQ  Q QQQ    QQQQQQ Q
Sbjct: 721 ------QLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQLPQIQQQ----QQQQQQPQ 780

Query: 781 QPQLSQLQTQQQIPQM----QQQQQQMVGGGISQSYVQGPGRSQLVSQGQVSSQGQSNMP 840
           QPQLSQLQTQQQIPQM    QQQQQQMVGGGI QSYVQGPGRSQLVSQGQV+SQGQSNMP
Sbjct: 781 QPQLSQLQTQQQIPQMQQQQQQQQQQMVGGGIGQSYVQGPGRSQLVSQGQVASQGQSNMP 826

Query: 841 GGSFMG 843
           GGSFMG
Sbjct: 841 GGSFMG 826

BLAST of Tan0013746 vs. NCBI nr
Match: KAG6573988.1 (Mediator of RNA polymerase II transcription subunit 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1368.2 bits (3540), Expect = 0.0e+00
Identity = 788/865 (91.10%), Postives = 807/865 (93.29%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVTDYI+KIVRCF GNEL+GQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQ 120
           GSYCGCLVQRTGWTRDVD FIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQ+GGQNQ
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 QTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCF 180
           QTMD+QKHCILVAASNPYPLPTPVYRP V +LEQ DNVEPQSG SQSYAETVAKSFAQCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGTSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGI 240
           ISLSVICPKQLPKLRAIY AGKRN RAADPPID  K PSYLVLISENFVEARAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 300
           TSL ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVS+ANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGIPQPLRPV 360
           MTNG AYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNG+ QPLRPV
Sbjct: 301 MTNGPAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420
           GPANVSILNNISQARV   ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

Query: 421 YSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQS 480
           +SSGQSG+TSINGS+TLTQVAPNSGLSSLSSA+NNIPGN+NIPTSQA+GNLQGGV+VSQS
Sbjct: 421 FSSGQSGLTSINGSSTLTQVAPNSGLSSLSSANNNIPGNTNIPTSQAVGNLQGGVNVSQS 480

Query: 481 VPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGV 540
           VPGMSQGNLAGTQVVQSGI MNQNVI NLSQPGV+SGNGTMIPTPGMPQQVQNGMHSLGV
Sbjct: 481 VPGMSQGNLAGTQVVQSGISMNQNVISNLSQPGVSSGNGTMIPTPGMPQQVQNGMHSLGV 540

Query: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600
           SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA
Sbjct: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600

Query: 601 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660
           ANWP  MQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL
Sbjct: 601 ANWPQIMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660

Query: 661 LLSVSDKACRLIGMLFPGDMVVFKPQIPS-QQQQQQQMQQQQQQQMQQQQ-QQQMQQQQM 720
           LLSVSDKACRLIGMLFPGDMVVFKPQI S QQQQQQQ+QQQQQQQMQQQQ QQQ+QQQ  
Sbjct: 661 LLSVSDKACRLIGMLFPGDMVVFKPQISSQQQQQQQQIQQQQQQQMQQQQMQQQLQQQHQ 720

Query: 721 QQQLQQHPQL------QQQHQLSQLQQHQQLPQMQQQQQQLPQMQQ-------QQQQQQQ 780
             QLQQH QL      QQQ Q    QQ QQ  QMQQQQQQLPQMQQ       QQQQQQQ
Sbjct: 721 LSQLQQHQQLPQMQQQQQQQQQQMQQQQQQQQQMQQQQQQLPQMQQQQQLPQIQQQQQQQ 780

Query: 781 QQQLPQLQQQQQQQ----QQPQLSQLQTQQQIPQM----QQQQQQMVGGGISQSYVQGPG 840
           QQQLPQ+QQQQQQQ    QQPQLSQLQTQQQIPQM    QQQQQQMVGGGISQSYVQGPG
Sbjct: 781 QQQLPQIQQQQQQQQQQPQQPQLSQLQTQQQIPQMQQQQQQQQQQMVGGGISQSYVQGPG 840

Query: 841 RSQLVSQGQVSSQGQSNMPGGSFMG 843
           RSQLVSQGQV+SQGQSNMPGGSFMG
Sbjct: 841 RSQLVSQGQVASQGQSNMPGGSFMG 862

BLAST of Tan0013746 vs. ExPASy TrEMBL
Match: A0A6J1G0U0 (Mediator of RNA polymerase II transcription subunit 25 OS=Cucurbita moschata OX=3662 GN=LOC111449642 PE=3 SV=1)

HSP 1 Score: 1388.2 bits (3592), Expect = 0.0e+00
Identity = 785/846 (92.79%), Postives = 804/846 (95.04%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVTDYI+KIVRCF GNEL+GQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQ 120
           GSYCGCLVQRTGWTRDVD FIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQ+GGQNQ
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 QTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCF 180
           QTMD+QKHCILVAASNPYPLPTPVYRP V +LEQ DNVEPQSG+SQSYAETVAKSFAQCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGI 240
           ISLSVICPKQLPKLRAIY AGKRN RAADPPID  K PSYLVLISENFVEARAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 300
           TSL ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVS+ANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGIPQPLRPV 360
           MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNG+ QPLRPV
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420
           GPANVSILNNISQARV   ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

Query: 421 YSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQS 480
           +SSGQSG+TSINGS+TLTQVAPNSGLSSLSSA+NNIPGN+NIPTSQA+GNLQGGV+VSQS
Sbjct: 421 FSSGQSGLTSINGSSTLTQVAPNSGLSSLSSANNNIPGNTNIPTSQAVGNLQGGVNVSQS 480

Query: 481 VPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGV 540
           VPGMSQGNLAGTQVVQSGI MNQNVI NLSQPGV+SGNGTMIPTPGMPQQVQNGMHSLGV
Sbjct: 481 VPGMSQGNLAGTQVVQSGISMNQNVISNLSQPGVSSGNGTMIPTPGMPQQVQNGMHSLGV 540

Query: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600
           SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA
Sbjct: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600

Query: 601 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660
           ANWP  MQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL
Sbjct: 601 ANWPQIMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660

Query: 661 LLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQMQQQQQQQMQQQQQQQMQQQQMQQ 720
           LLSVSDKACRLIGMLFPGDMVVFKPQI SQ       QQQQQQQ+QQQQQQQMQQQQMQQ
Sbjct: 661 LLSVSDKACRLIGMLFPGDMVVFKPQISSQ-------QQQQQQQIQQQQQQQMQQQQMQQ 720

Query: 721 QLQQHPQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQQQQQQQQLPQLQQQQQQQQ 780
                 QLQQQHQLSQLQQHQQLPQMQQQQQQLPQM  QQQQQQQQQQLPQ+ QQQQQ Q
Sbjct: 721 ------QLQQQHQLSQLQQHQQLPQMQQQQQQLPQM--QQQQQQQQQQLPQI-QQQQQPQ 780

Query: 781 QPQLSQLQTQQQIPQM----QQQQQQMVGGGISQSYVQGPGRSQLVSQGQVSSQGQSNMP 840
           QPQLSQLQTQQQIPQM    QQQQQQMVGGGI QSYVQGPGRSQLVSQGQV+SQGQSNMP
Sbjct: 781 QPQLSQLQTQQQIPQMQQQQQQQQQQMVGGGIGQSYVQGPGRSQLVSQGQVASQGQSNMP 827

Query: 841 GGSFMG 843
           GGSFMG
Sbjct: 841 GGSFMG 827

BLAST of Tan0013746 vs. ExPASy TrEMBL
Match: A0A6J1G0T3 (Mediator of RNA polymerase II transcription subunit 25 OS=Cucurbita moschata OX=3662 GN=LOC111449642 PE=3 SV=1)

HSP 1 Score: 1379.0 bits (3568), Expect = 0.0e+00
Identity = 788/877 (89.85%), Postives = 807/877 (92.02%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVTDYI+KIVRCF GNEL+GQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQ 120
           GSYCGCLVQRTGWTRDVD FIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQ+GGQNQ
Sbjct: 61  GSYCGCLVQRTGWTRDVDTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 QTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCF 180
           QTMD+QKHCILVAASNPYPLPTPVYRP V +LEQ DNVEPQSG+SQSYAETVAKSFAQCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGI 240
           ISLSVICPKQLPKLRAIY AGKRN RAADPPID  K PSYLVLISENFVEARAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 300
           TSL ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVS+ANVPPATVKVEPNTVTS
Sbjct: 241 TSLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGIPQPLRPV 360
           MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNG+ QPLRPV
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420
           GPANVSILNNISQARV   ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

Query: 421 YSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQS 480
           +SSGQSG+TSINGS+TLTQVAPNSGLSSLSSA+NNIPGN+NIPTSQA+GNLQGGV+VSQS
Sbjct: 421 FSSGQSGLTSINGSSTLTQVAPNSGLSSLSSANNNIPGNTNIPTSQAVGNLQGGVNVSQS 480

Query: 481 VPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGV 540
           VPGMSQGNLAGTQVVQSGI MNQNVI NLSQPGV+SGNGTMIPTPGMPQQVQNGMHSLGV
Sbjct: 481 VPGMSQGNLAGTQVVQSGISMNQNVISNLSQPGVSSGNGTMIPTPGMPQQVQNGMHSLGV 540

Query: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600
           SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA
Sbjct: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600

Query: 601 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660
           ANWP  MQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL
Sbjct: 601 ANWPQIMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660

Query: 661 LLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQMQQQQQQQMQQQQQQQMQQQQMQQ 720
           LLSVSDKACRLIGMLFPGDMVVFKPQI SQ       QQQQQQQ+QQQQQQQMQQQQMQQ
Sbjct: 661 LLSVSDKACRLIGMLFPGDMVVFKPQISSQ-------QQQQQQQIQQQQQQQMQQQQMQQ 720

Query: 721 QLQQHPQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQ------------------- 780
                 QLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQ                   
Sbjct: 721 ------QLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQQLPQMQQQQQQQQQQQQQL 780

Query: 781 -QQQQQQQLPQLQQQQQQQ-----------QQPQLSQLQTQQQIPQM----QQQQQQMVG 840
            Q QQQQQLPQ+QQQQQQQ           QQPQLSQLQTQQQIPQM    QQQQQQMVG
Sbjct: 781 PQMQQQQQLPQIQQQQQQQQLPQIQQQQQPQQPQLSQLQTQQQIPQMQQQQQQQQQQMVG 840

Query: 841 GGISQSYVQGPGRSQLVSQGQVSSQGQSNMPGGSFMG 843
           GGI QSYVQGPGRSQLVSQGQV+SQGQSNMPGGSFMG
Sbjct: 841 GGIGQSYVQGPGRSQLVSQGQVASQGQSNMPGGSFMG 861

BLAST of Tan0013746 vs. ExPASy TrEMBL
Match: A0A6J1HXI9 (Mediator of RNA polymerase II transcription subunit 25 OS=Cucurbita maxima OX=3661 GN=LOC111467555 PE=3 SV=1)

HSP 1 Score: 1377.1 bits (3563), Expect = 0.0e+00
Identity = 780/846 (92.20%), Postives = 799/846 (94.44%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVTDYI+KIVRCF GNEL+GQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQ 120
           GSYCGCLVQRTGWTRD D FIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQ+GGQNQ
Sbjct: 61  GSYCGCLVQRTGWTRDADTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 QTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCF 180
           QTMD+QKHCILVAASNPYPLPTPVYRP V +LEQ DNVEPQSG+SQSYAETVAKSFAQCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGI 240
           ISLSVICPKQLPKLRAIY AGKRN RAADPPID  K PSYLVLISENFVEARAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 300
           T+L ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVS+ANVPPATVKVEPNTVTS
Sbjct: 241 TTLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGIPQPLRPV 360
           MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNG+ QPLRPV
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420
           GPANVSILNNISQARV   ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

Query: 421 YSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQS 480
           +SSGQSG+TSINGS+TLTQVAPNSGLSSLSSA+NNIPGN+NIPTSQA+GNLQGGV+VSQS
Sbjct: 421 FSSGQSGLTSINGSSTLTQVAPNSGLSSLSSANNNIPGNTNIPTSQAVGNLQGGVNVSQS 480

Query: 481 VPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGV 540
           VPGMSQGNLAGTQVVQSGI MNQNVI NLSQPGV+SGNGTMIPTPGMPQQVQNGMHSLGV
Sbjct: 481 VPGMSQGNLAGTQVVQSGISMNQNVISNLSQPGVSSGNGTMIPTPGMPQQVQNGMHSLGV 540

Query: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600
           SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA
Sbjct: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600

Query: 601 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660
           ANWP  MQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL
Sbjct: 601 ANWPQIMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660

Query: 661 LLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQMQQQQQQQMQQQQQQQMQQQQMQQ 720
           LLSVSDKACRLIGMLFPGDMVVFKPQI SQ       QQQQQQQ+QQQQQQQMQQQQMQQ
Sbjct: 661 LLSVSDKACRLIGMLFPGDMVVFKPQISSQ-------QQQQQQQIQQQQQQQMQQQQMQQ 720

Query: 721 QLQQHPQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQQQQQQQQLPQLQQQQQQQQ 780
                 QLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQ  Q QQQ    QQQQQQ Q
Sbjct: 721 ------QLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQLPQIQQQ----QQQQQQPQ 780

Query: 781 QPQLSQLQTQQQIPQM----QQQQQQMVGGGISQSYVQGPGRSQLVSQGQVSSQGQSNMP 840
           QPQLSQLQTQQQIPQM    QQQQQQMVGGGI QSYVQGPGRSQLVSQGQV+SQGQSNMP
Sbjct: 781 QPQLSQLQTQQQIPQMQQQQQQQQQQMVGGGIGQSYVQGPGRSQLVSQGQVASQGQSNMP 826

Query: 841 GGSFMG 843
           GGSFMG
Sbjct: 841 GGSFMG 826

BLAST of Tan0013746 vs. ExPASy TrEMBL
Match: A0A6J1HT56 (Mediator of RNA polymerase II transcription subunit 25 OS=Cucurbita maxima OX=3661 GN=LOC111467555 PE=3 SV=1)

HSP 1 Score: 1364.4 bits (3530), Expect = 0.0e+00
Identity = 787/870 (90.46%), Postives = 809/870 (92.99%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVTDYI+KIVRCF GNEL+GQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQ 120
           GSYCGCLVQRTGWTRD D FIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQ+GGQNQ
Sbjct: 61  GSYCGCLVQRTGWTRDADTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 QTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCF 180
           QTMD+QKHCILVAASNPYPLPTPVYRP V +LEQ DNVEPQSG+SQSYAETVAKSFAQCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGI 240
           ISLSVICPKQLPKLRAIY AGKRN RAADPPID  K PSYLVLISENFVEARAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 300
           T+L ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVS+ANVPPATVKVEPNTVTS
Sbjct: 241 TTLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGIPQPLRPV 360
           MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNG+ QPLRPV
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420
           GPANVSILNNISQARV   ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

Query: 421 YSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQS 480
           +SSGQSG+TSINGS+TLTQVAPNSGLSSLSSA+NNIPGN+NIPTSQA+GNLQGGV+VSQS
Sbjct: 421 FSSGQSGLTSINGSSTLTQVAPNSGLSSLSSANNNIPGNTNIPTSQAVGNLQGGVNVSQS 480

Query: 481 VPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGV 540
           VPGMSQGNLAGTQVVQSGI MNQNVI NLSQPGV+SGNGTMIPTPGMPQQVQNGMHSLGV
Sbjct: 481 VPGMSQGNLAGTQVVQSGISMNQNVISNLSQPGVSSGNGTMIPTPGMPQQVQNGMHSLGV 540

Query: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600
           SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA
Sbjct: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600

Query: 601 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660
           ANWP  MQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL
Sbjct: 601 ANWPQIMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660

Query: 661 LLSVSDKACRLIGMLFPGDMVVFKPQIPS-QQQQQQQMQQQQQQQMQQQQ-QQQMQQQQM 720
           LLSVSDKACRLIGMLFPGDMVVFKPQI S QQQQQQQ+QQQQQQQMQQQQ QQQ+QQQ  
Sbjct: 661 LLSVSDKACRLIGMLFPGDMVVFKPQISSQQQQQQQQIQQQQQQQMQQQQMQQQLQQQHQ 720

Query: 721 QQQLQQH---PQLQQQHQLSQLQQHQQLPQMQQQ---------QQQLPQMQQQQQQQ--- 780
             QLQQH   PQ+QQQ Q  Q QQ QQLPQMQQQ         QQQ  QMQQQQQQQ   
Sbjct: 721 LSQLQQHQQLPQMQQQQQQQQQQQQQQLPQMQQQQMQQQQQQMQQQQQQMQQQQQQQQQQ 780

Query: 781 ----QQQQQLPQLQQQQQQQ---QQPQLSQLQTQQQIPQM----QQQQQQMVGGGISQSY 840
               QQQQQLPQ+QQQQQQQ   QQPQLSQLQTQQQIPQM    QQQQQQMVGGGI QSY
Sbjct: 781 LPQMQQQQQLPQIQQQQQQQQQPQQPQLSQLQTQQQIPQMQQQQQQQQQQMVGGGIGQSY 840

Query: 841 VQGPGRSQLVSQGQVSSQGQSNMPGGSFMG 843
           VQGPGRSQLVSQGQV+SQGQSNMPGGSFMG
Sbjct: 841 VQGPGRSQLVSQGQVASQGQSNMPGGSFMG 867

BLAST of Tan0013746 vs. ExPASy TrEMBL
Match: A0A6J1HWR7 (Mediator of RNA polymerase II transcription subunit 25 OS=Cucurbita maxima OX=3661 GN=LOC111467555 PE=3 SV=1)

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 751/842 (89.19%), Postives = 771/842 (91.57%), Query Frame = 0

Query: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTH 60
           MAEKQLIVVVEGTAAMGPFWQTVVTDYI+KIVRCF GNEL+GQKPPTSNVEFSLVTFHTH
Sbjct: 1   MAEKQLIVVVEGTAAMGPFWQTVVTDYIEKIVRCFGGNELTGQKPPTSNVEFSLVTFHTH 60

Query: 61  GSYCGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQ 120
           GSYCGCLVQRTGWTRD D FIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQ+GGQNQ
Sbjct: 61  GSYCGCLVQRTGWTRDADTFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQNGGQNQ 120

Query: 121 QTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCF 180
           QTMD+QKHCILVAASNPYPLPTPVYRP V +LEQ DNVEPQSG+SQSYAETVAKSFAQCF
Sbjct: 121 QTMDVQKHCILVAASNPYPLPTPVYRPTVPNLEQQDNVEPQSGSSQSYAETVAKSFAQCF 180

Query: 181 ISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGI 240
           ISLSVICPKQLPKLRAIY AGKRN RAADPPID  K PSYLVLISENFVEARAALSRPGI
Sbjct: 181 ISLSVICPKQLPKLRAIYNAGKRNLRAADPPIDYVKIPSYLVLISENFVEARAALSRPGI 240

Query: 241 TSLPANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 300
           T+L ANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVS+ANVPPATVKVEPNTVTS
Sbjct: 241 TTLSANQSPVKMDISPVVPVTGPPPTTTPSANGPIINRQPVSVANVPPATVKVEPNTVTS 300

Query: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGIPQPLRPV 360
           MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNG+ QPLRPV
Sbjct: 301 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLSSQEMITNNENTQDLKPLVNGVSQPLRPV 360

Query: 361 GPANVSILNNISQARVMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420
           GPANVSILNNISQARV   ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV
Sbjct: 361 GPANVSILNNISQARV---ATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVPAAQNV 420

Query: 421 YSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGVSVSQS 480
           +SSGQSG+TSINGS+TLTQVAPNSGLSSLSSA+NNIPGN+NIPTSQA+GNLQGGV+VSQS
Sbjct: 421 FSSGQSGLTSINGSSTLTQVAPNSGLSSLSSANNNIPGNTNIPTSQAVGNLQGGVNVSQS 480

Query: 481 VPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGMHSLGV 540
           VPGMSQGNLAGTQVVQSGI MNQNVI NLSQPGV+SGNGTMIPTPGMPQQVQNGMHSLGV
Sbjct: 481 VPGMSQGNLAGTQVVQSGISMNQNVISNLSQPGVSSGNGTMIPTPGMPQQVQNGMHSLGV 540

Query: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600
           SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA
Sbjct: 541 SNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASASDKLA 600

Query: 601 ANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660
           ANWP  MQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL
Sbjct: 601 ANWPQIMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTL 660

Query: 661 LLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQMQQQQQQQMQQQQQQQMQQQQMQQ 720
           LLSVSDKACRLIGMLFPGDMVVFKPQI SQ       QQQQQQQ+QQQQQQQMQQQQMQQ
Sbjct: 661 LLSVSDKACRLIGMLFPGDMVVFKPQISSQ-------QQQQQQQIQQQQQQQMQQQQMQQ 720

Query: 721 QLQQHPQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQQQQQQQQLPQLQQQQQQQQ 780
                 QLQQQHQLSQLQQHQQLPQMQQQQQQ+PQMQQQQ                    
Sbjct: 721 ------QLQQQHQLSQLQQHQQLPQMQQQQQQIPQMQQQQ-------------------- 780

Query: 781 QPQLSQLQTQQQIPQMQQQQQQMVGGGISQSYVQGPGRSQLVSQGQVSSQGQSNMPGGSF 840
                           QQQQQQMVGGGI QSYVQGPGRSQLVSQGQV+SQGQSNMPGGSF
Sbjct: 781 ----------------QQQQQQMVGGGIGQSYVQGPGRSQLVSQGQVASQGQSNMPGGSF 790

Query: 841 MG 843
           MG
Sbjct: 841 MG 790

BLAST of Tan0013746 vs. TAIR 10
Match: AT1G25540.1 (phytochrome and flowering time regulatory protein (PFT1) )

HSP 1 Score: 815.1 bits (2104), Expect = 5.3e-236
Identity = 530/861 (61.56%), Postives = 640/861 (74.33%), Query Frame = 0

Query: 4   KQLIVVVEGTAAMGPFWQTVVTDYIDKIVRCFCGNELSGQKPPTSNVEFSLVTFHTHGSY 63
           KQLIVV EGTAA+GP+WQT+V+DY++KI+R FCG+EL+G++ P S VE SLV F++HGSY
Sbjct: 6   KQLIVVAEGTAALGPYWQTIVSDYLEKIIRSFCGSELNGERNPVSTVELSLVIFNSHGSY 65

Query: 64  CGCLVQRTGWTRDVDIFIQWLSAIPFSGGGFSDAAIAEGLGEALMMFPTQQSGGQNQQTM 123
           C CLVQR+GWTRDVDIF+ WLS+I F GGGF++ A AEGL EALMMF      GQ Q + 
Sbjct: 66  CACLVQRSGWTRDVDIFLHWLSSIQFGGGGFNEVATAEGLAEALMMF--SPPSGQAQPSN 125

Query: 124 DMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQSYAETVAKSFAQCFISL 183
           D+++HCIL+ ASNP+ LPTPVYRP +Q++E+++N + Q+ +  S AETVA  FA+C +SL
Sbjct: 126 DLKRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLSDAETVASYFAKCSVSL 185

Query: 184 SVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISENFVEARAALSRPGITSL 243
           SV+CPKQLP +RA+Y AGK N ++AD  ID AK   YLVLISENFVEA AALS    T+L
Sbjct: 186 SVVCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSH-SATNL 245

Query: 244 PANQSPVKMD---ISPVVPVTGPPPTTTPSANGPIINRQPVSIANVPPATVKVEPNTVTS 303
           P  QSPVK+D   ++P +PVTG PP    SANGPI NRQPVS+  VP ATVKVEP+TVTS
Sbjct: 246 PQTQSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTS 305

Query: 304 MTNGSAYPPHMPSVVRAASQGVPSLQTSSPLS-SQEMITNNENTQDLKP-LVNGIPQPLR 363
           M    ++ PH+P+V R A+Q +PS+QTSS    SQ+M++N EN  D+KP +V+G+  PLR
Sbjct: 306 MAPVPSF-PHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENAPDIKPVVVSGMTPPLR 365

Query: 364 --PVGPANVSILNNISQAR-VMSSATLNGGTSIGLASMGQTPMAMHVSNMISSGMGSSVP 423
             P G ANV++LNN+SQ R VMSSA L G  S    S+GQ+ +AMH+SNMIS+GM +S+P
Sbjct: 366 TGPPGGANVNLLNNLSQVRQVMSSAALAGAAS----SVGQSAVAMHMSNMISTGMATSLP 425

Query: 424 AAQNVYSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGNSNIPTSQAIGNLQGGV 483
            +Q V+S+GQ G+TS+ GS  L            S+ +   PG +N  + Q   N+   +
Sbjct: 426 PSQTVFSTGQQGITSMAGSGALMG----------SAQTGQSPGPNNAFSPQTTSNVASNL 485

Query: 484 SVSQSVPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNGTMIPTPGMPQQVQNGM 543
            VSQ + GM+QG+ +G  ++Q GI MNQN++  L Q  V+SG G M+PTPG+ QQ Q+G+
Sbjct: 486 GVSQPMQGMNQGSHSGA-MMQGGISMNQNMMSGLGQGNVSSGTGGMMPTPGVGQQAQSGI 545

Query: 544 HSLGVSNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQGQPVFITRMEGYRSASA 603
             LG SN++A NM LSQ ++GA+Q +QSKYVK WEGNLSGQRQGQPV ITR+EGYRSASA
Sbjct: 546 QQLGGSNSSAPNMQLSQPSSGAMQTSQSKYVKVWEGNLSGQRQGQPVLITRLEGYRSASA 605

Query: 604 SDKLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQL 663
           SD LAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAM+QHGFLGQLQ+KKLCAVIQL
Sbjct: 606 SDSLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMSQHGFLGQLQDKKLCAVIQL 665

Query: 664 PSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQ---MQQQQQQQMQQQQQQQ 723
           PSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIP+QQQQQQQ    QQQQQQQ+QQQQQQQ
Sbjct: 666 PSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPNQQQQQQQQLHQQQQQQQQIQQQQQQQ 725

Query: 724 --MQQQQM---QQQLQQH-PQLQQQHQLSQLQQHQQLPQMQQQQQQLPQMQQQQQQQQQQ 783
             +QQQQM   QQQ QQH  Q QQQHQLSQLQ HQQ  Q QQQQQQ  Q+ Q Q   QQQ
Sbjct: 726 QHLQQQQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQQQQQQQHQLTQLQHHHQQQ 785

Query: 784 QQLPQLQQQQQQ-----QQQPQLSQLQTQQQIPQMQQQQQQMVGGGISQSYVQGPGRSQL 843
           QQ   L Q QQQ     Q Q Q S L   QQ    QQQ QQMV GG  Q++ Q PGRSQ 
Sbjct: 786 QQASPLNQMQQQTSPLNQMQQQTSPLNQMQQ----QQQPQQMVMGG--QAFAQAPGRSQ- 836

BLAST of Tan0013746 vs. TAIR 10
Match: AT1G25540.2 (phytochrome and flowering time regulatory protein (PFT1) )

HSP 1 Score: 650.6 bits (1677), Expect = 1.7e-186
Identity = 458/757 (60.50%), Postives = 550/757 (72.66%), Query Frame = 0

Query: 108 MMFPTQQSGGQNQQTMDMQKHCILVAASNPYPLPTPVYRPPVQSLEQHDNVEPQSGNSQS 167
           MMF      GQ Q + D+++HCIL+ ASNP+ LPTPVYRP +Q++E+++N + Q+ +  S
Sbjct: 1   MMF--SPPSGQAQPSNDLKRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLS 60

Query: 168 YAETVAKSFAQCFISLSVICPKQLPKLRAIYTAGKRNPRAADPPIDNAKAPSYLVLISEN 227
            AETVA  FA+C +SLSV+CPKQLP +RA+Y AGK N ++AD  ID AK   YLVLISEN
Sbjct: 61  DAETVASYFAKCSVSLSVVCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISEN 120

Query: 228 FVEARAALSRPGITSLPANQSPVKMD---ISPVVPVTGPPPTTTPSANGPIINRQPVSIA 287
           FVEA AALS    T+LP  QSPVK+D   ++P +PVTG PP    SANGPI NRQPVS+ 
Sbjct: 121 FVEACAALSH-SATNLPQTQSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVG 180

Query: 288 NVPPATVKVEPNTVTSMTNGSAYPPHMPSVVRAASQGVPSLQTSSPLS-SQEMITNNENT 347
            VP ATVKVEP+TVTSM    ++ PH+P+V R A+Q +PS+QTSS    SQ+M++N EN 
Sbjct: 181 PVPTATVKVEPSTVTSMAPVPSF-PHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENA 240

Query: 348 QDLKP-LVNGIPQPLR--PVGPANVSILNNISQAR-VMSSATLNGGTSIGLASMGQTPMA 407
            D+KP +V+G+  PLR  P G ANV++LNN+SQ R VMSSA L G  S    S+GQ+ +A
Sbjct: 241 PDIKPVVVSGMTPPLRTGPPGGANVNLLNNLSQVRQVMSSAALAGAAS----SVGQSAVA 300

Query: 408 MHVSNMISSGMGSSVPAAQNVYSSGQSGMTSINGSTTLTQVAPNSGLSSLSSASNNIPGN 467
           MH+SNMIS+GM +S+P +Q V+S+GQ G+TS+ GS  L            S+ +   PG 
Sbjct: 301 MHMSNMISTGMATSLPPSQTVFSTGQQGITSMAGSGALMG----------SAQTGQSPGP 360

Query: 468 SNIPTSQAIGNLQGGVSVSQSVPGMSQGNLAGTQVVQSGIGMNQNVIGNLSQPGVTSGNG 527
           +N  + Q   N+   + VSQ + GM+QG+ +G  ++Q GI MNQN++  L Q  V+SG G
Sbjct: 361 NNAFSPQTTSNVASNLGVSQPMQGMNQGSHSGA-MMQGGISMNQNMMSGLGQGNVSSGTG 420

Query: 528 TMIPTPGMPQQVQNGMHSLGVSNNAAANMPLSQHTAGALQQAQSKYVKFWEGNLSGQRQG 587
            M+PTPG+ QQ Q+G+  LG SN++A NM LSQ ++GA+Q +QSKYVK WEGNLSGQRQG
Sbjct: 421 GMMPTPGVGQQAQSGIQQLGGSNSSAPNMQLSQPSSGAMQTSQSKYVKVWEGNLSGQRQG 480

Query: 588 QPVFITRMEGYRSASASDKLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHG 647
           QPV ITR+EGYRSASASD LAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAM+QHG
Sbjct: 481 QPVLITRLEGYRSASASDSLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMSQHG 540

Query: 648 FLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPSQQQQQQQ--- 707
           FLGQLQ+KKLCAVIQLPSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIP+QQQQQQQ   
Sbjct: 541 FLGQLQDKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPNQQQQQQQQLH 600

Query: 708 MQQQQQQQMQQQQQQQ--MQQQQM---QQQLQQH-PQLQQQHQLSQLQQHQQLPQMQQQQ 767
            QQQQQQQ+QQQQQQQ  +QQQQM   QQQ QQH  Q QQQHQLSQLQ HQQ  Q QQQQ
Sbjct: 601 QQQQQQQQIQQQQQQQQHLQQQQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQQQQ 660

Query: 768 QQLPQMQQQQQQQQQQQQLPQLQQQQQQ-----QQQPQLSQLQTQQQIPQMQQQQQQMVG 827
           QQ  Q+ Q Q   QQQQQ   L Q QQQ     Q Q Q S L   QQ    QQQ QQMV 
Sbjct: 661 QQQHQLTQLQHHHQQQQQASPLNQMQQQTSPLNQMQQQTSPLNQMQQ----QQQPQQMVM 720

Query: 828 GGISQSYVQGPGRSQLVSQGQVSSQGQSNMPGGSFMG 843
           GG  Q++ Q PGRSQ     Q    GQ NMPG  FMG
Sbjct: 721 GG--QAFAQAPGRSQ-----QGGGGGQPNMPGAGFMG 727