Tan0013732 (gene) Snake gourd v1

BLAST of Tan0013732 vs. ExPASy Swiss-Prot
Match: Q84K34 (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.5e-41
Identity = 91/286 (31.82%), Postives = 152/286 (53.15%), Query Frame = 0

Query: 10  KSMATRIVQNDQEAQSEETEEENAAVNTPEPKTSVKTTGTKRKIESQNVTLIDPEVLDCY 69
           ++   R    D+ + S++++ E         K     + +  K    +VTL+DP+VLDC 
Sbjct: 55  QNQGMRPESEDRGSTSDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCP 114

Query: 70  ICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRIGDIRCRAIENVLESLKGACQNS 129
           ICCEPL IP+FQC+NGH+AC+ CC+K    CP C   IG +RCRA+E V+E+ + +C N+
Sbjct: 115 ICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNA 174

Query: 130 KHGCKSKLTFDQRKEHWNTCSFVPCPCPLSDCNFVASAKKLYAHFNQKHPSSAINFQYYS 189
           K+GCK   ++  R  H   C F PC CP+ DC++    K L  H   +H    I+F + +
Sbjct: 175 KYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNT 234

Query: 190 SFVVDLNLDDKFLVFREKRAEILFVLSSRTQPFGKAITVNSIG-LASNKEGLSLELVMKT 249
              + L+L+ K  + +E+    + V+          ++V+ I  L      LS  L   T
Sbjct: 235 RLTISLDLNKKTTILQEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKIT 294

Query: 250 KETTLSLQSGMKGIKELVDELPPEEFLMIPNKFFGSDKQIKLEVRI 295
            ++ L     +K I+++ +E P + F++IP+  F  +  + L++ I
Sbjct: 295 VDSLLKQGFMVKNIQKVTNEHPEDGFMLIPSYLFSGNDNLNLQIWI 340

BLAST of Tan0013732 vs. ExPASy Swiss-Prot
Match: Q9FKD7 (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 5.4e-39
Identity = 91/253 (35.97%), Postives = 134/253 (52.96%), Query Frame = 0

Query: 50  KRKIESQNVTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRIGD 109
           KR  + ++  L+D E+LDC IC E   IP+FQC+NGH+ACS CC K   +CP C   +G 
Sbjct: 33  KRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGH 92

Query: 110 IRCRAIENVLESLKGACQNSKHGCKSKLTFDQRKEHWNTCSFVPCPCPLSDCNFVASAKK 169
            RCRA+E+VLES+   C N+K GCK  +++ +   H   C F  C CP  DCN+ +S K 
Sbjct: 93  NRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKD 152

Query: 170 LYAHFNQKHPSSAINFQYYSSFVVD------LNLDDKFLVFREKRAEILFVLSSRTQPFG 229
           LY H+   H    +     ++F+ D      +N+  K L+  E     LF +    +P+G
Sbjct: 153 LYTHYRITH----MEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFREPYG 212

Query: 230 KAITVNSIGLASNK-EGLSLELVMKTKETTLSLQS-GMKGIKELVDELPPEEFLMIPNKF 289
             +TV+ I  +S +    S  L       T+  QS  +K + +L  + P E F++IPN  
Sbjct: 213 VYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQENFMLIPNSL 272

Query: 290 FGSDKQIKLEVRI 295
              D    LE+RI
Sbjct: 273 LRGD---VLEMRI 278

BLAST of Tan0013732 vs. ExPASy Swiss-Prot
Match: Q7XA77 (E3 ubiquitin-protein ligase SINA-like 5 OS=Arabidopsis thaliana OX=3702 GN=At1g66660 PE=2 SV=2)

HSP 1 Score: 161.4 bits (407), Expect = 1.6e-38
Identity = 104/276 (37.68%), Postives = 149/276 (53.99%), Query Frame = 0

Query: 18  QNDQEAQSEETEEENAAVNTPEPKTSVKTTGTKRKIESQNVTLIDPEVLDCYICCEPLAI 77
           + + E + +E EEE   V T E   S K+        SQ V L   +VLDC  CCEPL  
Sbjct: 44  EEELEEEEDEEEEEEENVTTDEQSGSPKS--------SQPVKLQSSDVLDCPTCCEPLKR 103

Query: 78  PVFQCENGHVACSDCCSKNVEECPFCHKRIGDIRCRAIENVLESLKGACQNSKHGCKSKL 137
           P++QC NGH+ACS CC K  ++C FC   IGDIRCRA+E V+E+    C N+KHGCK   
Sbjct: 104 PIYQCSNGHLACSSCCQKLNKKCSFCRCNIGDIRCRAMEKVIEASIVPCPNAKHGCKETT 163

Query: 138 TFDQRKEHWNTCSFVPCPCPLSDCNFVASAKKLYAH-FNQKH--PSSAINFQYY--SSFV 197
           T+  +  H   C FV C CP+S+CN+V+S   L +H  +  H      I+FQ       +
Sbjct: 164 TYCNQSSHEKVCKFVRCSCPVSNCNYVSSYSNLKSHACSTAHVWGEDDIHFQLVIDRPRI 223

Query: 198 VDLNLD-DKFLVFREKRAEILFVLSSRTQPFGKAITVNSIG-LASNKEGLSLELVMKTKE 257
            ++NL   K +VF+E++   L V+ +     G  +TVN I  +A     LS  L    + 
Sbjct: 224 FNMNLGRKKTVVFKEEKEGDLIVVQAFKGLEGVYVTVNRIAHMAPGIRDLSCSLAKLNEY 283

Query: 258 TTLSLQSGMKGIKELVDELPPEEFLM-IPNKFFGSD 286
           +TL   S +K I+++ +++  E+ LM IP K    D
Sbjct: 284 STLRSGSLVKKIQKVREKMHLEDDLMWIPPKMLSGD 311

BLAST of Tan0013732 vs. ExPASy Swiss-Prot
Match: Q9FKD9 (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.0e-38
Identity = 86/237 (36.29%), Postives = 135/237 (56.96%), Query Frame = 0

Query: 48  GTKRKIESQNVTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRI 107
           G KR  ++++  L D ++LDC IC + L IPVFQC NGH+ACS CC K   +CP C   +
Sbjct: 25  GRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPV 84

Query: 108 GDIRCRAIENVLESLKGACQNSKHGCKSKLTFDQRKEHWNTCSFVPCPCPLSDCNFVASA 167
           G IRCRA+E VLES+   C+ +  GC   + + +   H   C+F PC CP+  CN+  S 
Sbjct: 85  GHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSY 144

Query: 168 KKLYAHFNQKHP--SSAINFQYYSSFVVDLNLDDKFLVFREKRAEILFVLSSRTQPFGKA 227
           K LY H++  H   S+A +F   S     + + DK L+ R    ++LFV+    +P G  
Sbjct: 145 KDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQCFEEPCGVY 204

Query: 228 ITVNSIGLASNKEG-LSLELVMKTKE-TTLSLQS-GMKGIKELVDELPPEEFLMIPN 280
           ++V+ I  ++ + G  S  L+  T E  T++ QS  +K + ++  + P + F++IP+
Sbjct: 205 VSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIPH 261

BLAST of Tan0013732 vs. ExPASy Swiss-Prot
Match: Q9C9M0 (E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana OX=3702 GN=At1g66650 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.0e-37
Identity = 102/306 (33.33%), Postives = 158/306 (51.63%), Query Frame = 0

Query: 5   RISFRKSMATRIVQNDQEAQSEETE------EENAAVNTPEPKTSVKTTGTKRKIES-QN 64
           R S ++   T +  +D     EE +      +++ +    +P   V  T  KR++ S ++
Sbjct: 16  RSSTKRQRRTSVSVDDPSPGEEEEKTLVVLTDDSDSEEDDKPLGDVLRTCRKRRVSSPKS 75

Query: 65  VTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRIGDIRCRAIEN 124
           VTL +  VL+C  C +PL  P+FQC NGH+AC  CC K  + C FC   IGD+RCRA+E 
Sbjct: 76  VTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRCRAMEK 135

Query: 125 VLESLKGACQNSKHGCKSKLTF-DQRKEHWNTCSFVPCPCPLSDCNFVASAKKLYAHFNQ 184
           V+++   +C N+ +GCK   T+ +Q + H   C F PC CP+ DCN++   K L  HF  
Sbjct: 136 VIKAGLVSCSNAIYGCKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIGFYKDLINHFRA 195

Query: 185 KH---PSSAINFQYYSSFVVDLNLD--DKFLVFREKRAEILFVLSSRTQPFGKAITVNSI 244
            H   P    +F +    +  L+LD  DK ++F E++   LFV+       G   TV+ I
Sbjct: 196 THKVSPGDINSFVFDRPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGFIGSHGVYATVSHI 255

Query: 245 G-LASNKEGLSLELVMKTKETTLSLQSGMKGIKEL--VDELPPEEFLMIPNKFFGSDKQI 295
             +       S  L      +TL L   +K I++L   +E P E+FL+IP+     D  +
Sbjct: 256 APMVPEVRKFSCSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIPSYMVNGD-HM 315

BLAST of Tan0013732 vs. NCBI nr
Match: XP_030952500.1 (E3 ubiquitin-protein ligase SINA-like 10 [Quercus lobata])

HSP 1 Score: 253.1 bits (645), Expect = 3.0e-63
Identity = 133/281 (47.33%), Postives = 181/281 (64.41%), Query Frame = 0

Query: 21  QEAQSEETEEENAAVN-----TPEPKTSVKTTGTKRKI--ESQNVTLIDPEVLDCYICCE 80
           +E + +E EEE    N     T  P++++   G    +   S +VTL DPEVLDC IC E
Sbjct: 83  EEVEEDEEEEEAQQGNQGSDVTVGPRSTIGNDGHVIHVGDGSISVTLTDPEVLDCSICLE 142

Query: 81  PLAIPVFQCENGHVACSDCCSKNVEECPFCHKRIGDIRCRAIENVLESLKGACQNSKHGC 140
            L+ PVFQCENGH+ACS CC+K   +CP C   IG  RCRAIE VLE++K  CQNSK+GC
Sbjct: 143 HLSPPVFQCENGHIACSSCCNKLKNKCPSCFWPIGYNRCRAIEKVLEAVKIPCQNSKYGC 202

Query: 141 KSKLTFDQRKEHWNTCSFVPCPCPLSDCNFVASAKKLYAHFNQKHPSSAINFQYYSSFVV 200
           K  +++ ++ +H  TC + PC CPLS C F+AS+K++Y HF+ KH +SAI F+Y S+F V
Sbjct: 203 KEVVSYSRKSDHEKTCIYAPCSCPLSHCKFIASSKQIYVHFSSKHKNSAIGFKYDSNFSV 262

Query: 201 DLNLDDKFLVFREKRAEILFVLSSRTQPFGKAITVNSIGLASNKEGLSLELVMKTKETTL 260
            L L DKFLV +E+   +LFVL++     G A+T+N +G +S   G S  L +KT+ ++L
Sbjct: 263 SLTLTDKFLVLQEQSNGVLFVLNNCVDHRGNAVTINCVGPSSTTGGFSYGLAVKTQGSSL 322

Query: 261 SLQSGMKGIKELVDELPPEEFLMIPNKFFGSDKQIKLEVRI 295
            L+S  K  +  VD  P   FL+IP  FFGS  Q+KLEV I
Sbjct: 323 RLESFTKNSQHRVDSPPSTGFLLIPTDFFGSCGQLKLEVYI 363

BLAST of Tan0013732 vs. NCBI nr
Match: KAG6606997.1 (E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 252.7 bits (644), Expect = 4.0e-63
Identity = 121/258 (46.90%), Postives = 170/258 (65.89%), Query Frame = 0

Query: 39  EPKTSVKTTGTKRKIESQNVTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVE 98
           E +       ++RK E+  V L DP+VLDCYICCEPL++PV+QCENGH++CS CCSK   
Sbjct: 10  EKERRSSANSSRRKNETMKVILTDPQVLDCYICCEPLSVPVYQCENGHISCSSCCSKVQN 69

Query: 99  ECPFCHKRIGDIRCRAIENVLESLKGACQNSKHGCKSKLTFDQRKEHWNTCSFVPCPCPL 158
           +CP C   IG +RCRAIE VLES+K  CQNS++GCK+    +Q  EH   C + PC CPL
Sbjct: 70  KCPSCSLAIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQPCSCPL 129

Query: 159 SDCNFVASAKKLYAHFNQKHPSSAINFQYYSSFVVDLNLDDKFLVFREKRAEILFVLSSR 218
           SDC++V SA+ L +HF ++H  SA +  Y S F + LN DDK+ +F+ +   +LFVLS  
Sbjct: 130 SDCSYVDSAEYLSSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVLFVLSYS 189

Query: 219 TQPFGKAITVNSIGLASNKEGLSLELVMKTKETTLSLQSGMKGIKELVDELPPEEFLMIP 278
            + FG A+T+N IG  S+++    ++  K++ + LSL S  K I+   ++ P +  L+IP
Sbjct: 190 FETFGDAVTLNRIGPRSSEKKFCYDIKAKSQGSVLSLLSVAKEIQRWTEDPPSKGSLLIP 249

Query: 279 NKFFGSDK-QIKLEVRIW 296
           N+FFGS   Q  LE+RIW
Sbjct: 250 NEFFGSSSAQTILEIRIW 267

BLAST of Tan0013732 vs. NCBI nr
Match: XP_022949045.1 (uncharacterized protein LOC111452508 [Cucurbita moschata])

HSP 1 Score: 252.7 bits (644), Expect = 4.0e-63
Identity = 121/258 (46.90%), Postives = 170/258 (65.89%), Query Frame = 0

Query: 39  EPKTSVKTTGTKRKIESQNVTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVE 98
           E +       ++RK E+  V L DP+VLDCYICCEPL++PV+QCENGH++CS CCSK   
Sbjct: 280 EKERRSSANSSRRKNETMKVILTDPQVLDCYICCEPLSVPVYQCENGHISCSSCCSKVQN 339

Query: 99  ECPFCHKRIGDIRCRAIENVLESLKGACQNSKHGCKSKLTFDQRKEHWNTCSFVPCPCPL 158
           +CP C   IG +RCRAIE VLES+K  CQNS++GCK+    +Q  EH   C + PC CPL
Sbjct: 340 KCPSCSLAIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQPCSCPL 399

Query: 159 SDCNFVASAKKLYAHFNQKHPSSAINFQYYSSFVVDLNLDDKFLVFREKRAEILFVLSSR 218
           SDC++V SA+ L +HF ++H  SA +  Y S F + LN DDK+ +F+ +   +LFVLS  
Sbjct: 400 SDCSYVDSAEYLSSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVLFVLSYS 459

Query: 219 TQPFGKAITVNSIGLASNKEGLSLELVMKTKETTLSLQSGMKGIKELVDELPPEEFLMIP 278
            + FG A+T+N IG  S+++    ++  K++ + LSL S  K I+   ++ P +  L+IP
Sbjct: 460 FETFGDAVTLNRIGPRSSEKKFCYDIKAKSQGSVLSLLSVAKEIQRWTEDPPSKGSLLIP 519

Query: 279 NKFFGSDK-QIKLEVRIW 296
           N+FFGS   Q  LE+RIW
Sbjct: 520 NEFFGSSSAQTILEIRIW 537

BLAST of Tan0013732 vs. NCBI nr
Match: XP_022143325.1 (E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia])

HSP 1 Score: 251.5 bits (641), Expect = 8.8e-63
Identity = 120/244 (49.18%), Postives = 164/244 (67.21%), Query Frame = 0

Query: 52  KIESQNVTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRIGDIR 111
           K E+ NV   DP++LDCYICCEPL+ PVFQCENGH+ACS CC+K   +CP C   IG IR
Sbjct: 22  KTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIR 81

Query: 112 CRAIENVLESLKGACQNSKHGCKSKLTFDQRKEHWNTCSFVPCPCPLSDCNFVASAKKLY 171
           CRAIE VLES+K  C NS +GCK  +T ++R +H + C++ PC CP+SDC FV S++KL 
Sbjct: 82  CRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLC 141

Query: 172 AHFNQKHPSSAINFQYYSSFVVDLNLDDKFLVFREKRAEILFVLSSRTQPFGKAITVNSI 231
           +HF++KH +SA +F Y S F + LN  D + + + ++   LF LS   + FG A+TV+ I
Sbjct: 142 SHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRI 201

Query: 232 GLASNKEGLSLELVMKTKETTLSLQSGMKGIKELVDELPPEEFLMIPNKFFGSDKQIKLE 291
           G +S+++    E+  KT+ + L LQS  K I+  V+  P    L+IPN+FFGS  Q  LE
Sbjct: 202 GPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPPSNRSLLIPNEFFGSSAQTILE 261

Query: 292 VRIW 296
            RIW
Sbjct: 262 FRIW 265

BLAST of Tan0013732 vs. NCBI nr
Match: XP_022143330.1 (E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia])

HSP 1 Score: 250.0 bits (637), Expect = 2.6e-62
Identity = 117/168 (69.64%), Postives = 138/168 (82.14%), Query Frame = 0

Query: 21  QEAQSEETEEENAAVNTPEPKTSVKTTGTKRKIESQNVTLIDPEVLDCYICCEPLAIPVF 80
           Q AQ EE EE     N P+ +++  T+  KR+IESQNVT I+PEVLDCYICCEPLAIPVF
Sbjct: 9   QSAQREEAEE-----NPPDSESNANTSSKKRRIESQNVT-INPEVLDCYICCEPLAIPVF 68

Query: 81  QCENGHVACSDCCSKNVEECPFCHKRIGDIRCRAIENVLESLKGACQNSKHGCKSKLTFD 140
           QCENGH+ACSDCCS+NV+ECP CH +I +IRCRAIE VLESL+ +CQN ++ CK KLTF 
Sbjct: 69  QCENGHIACSDCCSRNVKECPSCHIKIDEIRCRAIEIVLESLERSCQNXRYCCKEKLTFG 128

Query: 141 QRKEHWNTCSFVPCPCPLSDCNFVASAKKLYAHFNQKHPSSAINFQYY 189
           QRKEH NTC F PCPCPL DC FVAS+KKLY+HFNQKHP+SA NF+Y+
Sbjct: 129 QRKEHLNTCLFSPCPCPLFDCEFVASSKKLYSHFNQKHPTSAFNFEYW 170

BLAST of Tan0013732 vs. ExPASy TrEMBL
Match: A0A6J1GBP1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.9e-63
Identity = 121/258 (46.90%), Postives = 170/258 (65.89%), Query Frame = 0

Query: 39  EPKTSVKTTGTKRKIESQNVTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVE 98
           E +       ++RK E+  V L DP+VLDCYICCEPL++PV+QCENGH++CS CCSK   
Sbjct: 280 EKERRSSANSSRRKNETMKVILTDPQVLDCYICCEPLSVPVYQCENGHISCSSCCSKVQN 339

Query: 99  ECPFCHKRIGDIRCRAIENVLESLKGACQNSKHGCKSKLTFDQRKEHWNTCSFVPCPCPL 158
           +CP C   IG +RCRAIE VLES+K  CQNS++GCK+    +Q  EH   C + PC CPL
Sbjct: 340 KCPSCSLAIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQPCSCPL 399

Query: 159 SDCNFVASAKKLYAHFNQKHPSSAINFQYYSSFVVDLNLDDKFLVFREKRAEILFVLSSR 218
           SDC++V SA+ L +HF ++H  SA +  Y S F + LN DDK+ +F+ +   +LFVLS  
Sbjct: 400 SDCSYVDSAEYLSSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVLFVLSYS 459

Query: 219 TQPFGKAITVNSIGLASNKEGLSLELVMKTKETTLSLQSGMKGIKELVDELPPEEFLMIP 278
            + FG A+T+N IG  S+++    ++  K++ + LSL S  K I+   ++ P +  L+IP
Sbjct: 460 FETFGDAVTLNRIGPRSSEKKFCYDIKAKSQGSVLSLLSVAKEIQRWTEDPPSKGSLLIP 519

Query: 279 NKFFGSDK-QIKLEVRIW 296
           N+FFGS   Q  LE+RIW
Sbjct: 520 NEFFGSSSAQTILEIRIW 537

BLAST of Tan0013732 vs. ExPASy TrEMBL
Match: A0A6J1CNI1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013226 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 4.3e-63
Identity = 120/244 (49.18%), Postives = 164/244 (67.21%), Query Frame = 0

Query: 52  KIESQNVTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRIGDIR 111
           K E+ NV   DP++LDCYICCEPL+ PVFQCENGH+ACS CC+K   +CP C   IG IR
Sbjct: 22  KTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIR 81

Query: 112 CRAIENVLESLKGACQNSKHGCKSKLTFDQRKEHWNTCSFVPCPCPLSDCNFVASAKKLY 171
           CRAIE VLES+K  C NS +GCK  +T ++R +H + C++ PC CP+SDC FV S++KL 
Sbjct: 82  CRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLC 141

Query: 172 AHFNQKHPSSAINFQYYSSFVVDLNLDDKFLVFREKRAEILFVLSSRTQPFGKAITVNSI 231
           +HF++KH +SA +F Y S F + LN  D + + + ++   LF LS   + FG A+TV+ I
Sbjct: 142 SHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRI 201

Query: 232 GLASNKEGLSLELVMKTKETTLSLQSGMKGIKELVDELPPEEFLMIPNKFFGSDKQIKLE 291
           G +S+++    E+  KT+ + L LQS  K I+  V+  P    L+IPN+FFGS  Q  LE
Sbjct: 202 GPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVPPSNRSLLIPNEFFGSSAQTILE 261

Query: 292 VRIW 296
            RIW
Sbjct: 262 FRIW 265

BLAST of Tan0013732 vs. ExPASy TrEMBL
Match: A0A6J1CNI6 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013228 PE=3 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 1.2e-62
Identity = 117/168 (69.64%), Postives = 138/168 (82.14%), Query Frame = 0

Query: 21  QEAQSEETEEENAAVNTPEPKTSVKTTGTKRKIESQNVTLIDPEVLDCYICCEPLAIPVF 80
           Q AQ EE EE     N P+ +++  T+  KR+IESQNVT I+PEVLDCYICCEPLAIPVF
Sbjct: 9   QSAQREEAEE-----NPPDSESNANTSSKKRRIESQNVT-INPEVLDCYICCEPLAIPVF 68

Query: 81  QCENGHVACSDCCSKNVEECPFCHKRIGDIRCRAIENVLESLKGACQNSKHGCKSKLTFD 140
           QCENGH+ACSDCCS+NV+ECP CH +I +IRCRAIE VLESL+ +CQN ++ CK KLTF 
Sbjct: 69  QCENGHIACSDCCSRNVKECPSCHIKIDEIRCRAIEIVLESLERSCQNXRYCCKEKLTFG 128

Query: 141 QRKEHWNTCSFVPCPCPLSDCNFVASAKKLYAHFNQKHPSSAINFQYY 189
           QRKEH NTC F PCPCPL DC FVAS+KKLY+HFNQKHP+SA NF+Y+
Sbjct: 129 QRKEHLNTCLFSPCPCPLFDCEFVASSKKLYSHFNQKHPTSAFNFEYW 170

BLAST of Tan0013732 vs. ExPASy TrEMBL
Match: A0A6J1K8G5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 PE=3 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 2.3e-61
Identity = 116/248 (46.77%), Postives = 167/248 (67.34%), Query Frame = 0

Query: 49  TKRKIESQNVTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRIG 108
           ++ K E+  V   DP++LDCYICCEPL++PV+QCENGH++CS CCSK   +CP C   IG
Sbjct: 20  SRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSCCSKVHNKCPSCSLAIG 79

Query: 109 DIRCRAIENVLESLKGACQNSKHGCKSKLTFDQRKEHWNTCSFVPCPCPLSDCNFVASAK 168
            IRCRAIE VLES+K  CQNSK+GCK  +  +Q  EH  +C + PC CP+SDC++V SA+
Sbjct: 80  YIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSCPYEPCSCPISDCSYVDSAE 139

Query: 169 KLYAHFNQKHPSSAINFQYYSSFVVDLNLDDKFLVFREKRAEILFVLSSRTQPFGKAITV 228
            L +HF ++H  SA +F Y S F + LN  D++ +F+ +  + LFVLS   + FG A+T+
Sbjct: 140 HLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAENDDALFVLSYSFEIFGNAVTL 199

Query: 229 NSIGLASNKEGLSLELVMKTKETTLSLQSGMKGIKELVDELPPEEFLMIPNKFFGSDK-Q 288
           + IG   +++    ++  K++ + LSLQS  K I+   ++ P +  L+IPN+FFGS   Q
Sbjct: 200 SRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDPPFKGSLLIPNEFFGSSSAQ 259

Query: 289 IKLEVRIW 296
             LE+RIW
Sbjct: 260 TILEIRIW 267

BLAST of Tan0013732 vs. ExPASy TrEMBL
Match: A0A6J1GYB0 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111458302 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 5.2e-61
Identity = 127/274 (46.35%), Postives = 167/274 (60.95%), Query Frame = 0

Query: 22  EAQSEETEEENAAVNTPEPKTSVKTTGTKRKIESQNVTLIDPEVLDCYICCEPLAIPVFQ 81
           E Q EE   ENA  +      +VK  G      S ++ LIDPEVLDC ICCEPL++P+FQ
Sbjct: 76  EEQVEEDGTENALCSVKSCSRNVKENG------SISMFLIDPEVLDCSICCEPLSVPIFQ 135

Query: 82  CENGHVACSDCCSKNVEECPFCHKRIGDIRCRAIENVLESLKGACQNSKHGCKSKLTFDQ 141
           CENGH+ACS CC+K    C  C   IG  RCRA+E VLES+K  C+ SK+GCK  +++  
Sbjct: 136 CENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGS 195

Query: 142 RKEHWNTCSFVPCPCPLSDCNFVASAKKLYAHFNQKHPSSAINFQYYSSFVVDLNLDDKF 201
             +H   C   PC CPL DCNFVAS+K+L  HF+ KH  SAI+FQ+ SSF + LN +D +
Sbjct: 196 ESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTDSAISFQFSSSFTICLNANDNY 255

Query: 202 LVFREKRAEILFVLSSRTQPFGKAITVNSIGLASNKEGLSLELVMKTKETTLSLQSGMKG 261
            V +E+   ILF+LS+     G A+ V  +   S KE  S +L ++T++ +L LQS MK 
Sbjct: 256 RVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPSLKEAFSYDLRVETQDLSLVLQSSMKN 315

Query: 262 IKELVDELPPEEFLMIPNKFFGSDKQIKLEVRIW 296
           I+   D  P   FL+IP   F S   IKL + IW
Sbjct: 316 IQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIW 343

BLAST of Tan0013732 vs. TAIR 10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-42
Identity = 91/286 (31.82%), Postives = 152/286 (53.15%), Query Frame = 0

Query: 10  KSMATRIVQNDQEAQSEETEEENAAVNTPEPKTSVKTTGTKRKIESQNVTLIDPEVLDCY 69
           ++   R    D+ + S++++ E         K     + +  K    +VTL+DP+VLDC 
Sbjct: 55  QNQGMRPESEDRGSTSDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCP 114

Query: 70  ICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRIGDIRCRAIENVLESLKGACQNS 129
           ICCEPL IP+FQC+NGH+AC+ CC+K    CP C   IG +RCRA+E V+E+ + +C N+
Sbjct: 115 ICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNA 174

Query: 130 KHGCKSKLTFDQRKEHWNTCSFVPCPCPLSDCNFVASAKKLYAHFNQKHPSSAINFQYYS 189
           K+GCK   ++  R  H   C F PC CP+ DC++    K L  H   +H    I+F + +
Sbjct: 175 KYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNT 234

Query: 190 SFVVDLNLDDKFLVFREKRAEILFVLSSRTQPFGKAITVNSIG-LASNKEGLSLELVMKT 249
              + L+L+ K  + +E+    + V+          ++V+ I  L      LS  L   T
Sbjct: 235 RLTISLDLNKKTTILQEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKIT 294

Query: 250 KETTLSLQSGMKGIKELVDELPPEEFLMIPNKFFGSDKQIKLEVRI 295
            ++ L     +K I+++ +E P + F++IP+  F  +  + L++ I
Sbjct: 295 VDSLLKQGFMVKNIQKVTNEHPEDGFMLIPSYLFSGNDNLNLQIWI 340

BLAST of Tan0013732 vs. TAIR 10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 162.9 bits (411), Expect = 3.8e-40
Identity = 91/253 (35.97%), Postives = 134/253 (52.96%), Query Frame = 0

Query: 50  KRKIESQNVTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRIGD 109
           KR  + ++  L+D E+LDC IC E   IP+FQC+NGH+ACS CC K   +CP C   +G 
Sbjct: 33  KRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGH 92

Query: 110 IRCRAIENVLESLKGACQNSKHGCKSKLTFDQRKEHWNTCSFVPCPCPLSDCNFVASAKK 169
            RCRA+E+VLES+   C N+K GCK  +++ +   H   C F  C CP  DCN+ +S K 
Sbjct: 93  NRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKD 152

Query: 170 LYAHFNQKHPSSAINFQYYSSFVVD------LNLDDKFLVFREKRAEILFVLSSRTQPFG 229
           LY H+   H    +     ++F+ D      +N+  K L+  E     LF +    +P+G
Sbjct: 153 LYTHYRITH----MEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFREPYG 212

Query: 230 KAITVNSIGLASNK-EGLSLELVMKTKETTLSLQS-GMKGIKELVDELPPEEFLMIPNKF 289
             +TV+ I  +S +    S  L       T+  QS  +K + +L  + P E F++IPN  
Sbjct: 213 VYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQENFMLIPNSL 272

Query: 290 FGSDKQIKLEVRI 295
              D    LE+RI
Sbjct: 273 LRGD---VLEMRI 278

BLAST of Tan0013732 vs. TAIR 10
Match: AT1G66660.2 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 161.4 bits (407), Expect = 1.1e-39
Identity = 104/276 (37.68%), Postives = 149/276 (53.99%), Query Frame = 0

Query: 18  QNDQEAQSEETEEENAAVNTPEPKTSVKTTGTKRKIESQNVTLIDPEVLDCYICCEPLAI 77
           + + E + +E EEE   V T E   S K+        SQ V L   +VLDC  CCEPL  
Sbjct: 64  EEELEEEEDEEEEEEENVTTDEQSGSPKS--------SQPVKLQSSDVLDCPTCCEPLKR 123

Query: 78  PVFQCENGHVACSDCCSKNVEECPFCHKRIGDIRCRAIENVLESLKGACQNSKHGCKSKL 137
           P++QC NGH+ACS CC K  ++C FC   IGDIRCRA+E V+E+    C N+KHGCK   
Sbjct: 124 PIYQCSNGHLACSSCCQKLNKKCSFCRCNIGDIRCRAMEKVIEASIVPCPNAKHGCKETT 183

Query: 138 TFDQRKEHWNTCSFVPCPCPLSDCNFVASAKKLYAH-FNQKH--PSSAINFQYY--SSFV 197
           T+  +  H   C FV C CP+S+CN+V+S   L +H  +  H      I+FQ       +
Sbjct: 184 TYCNQSSHEKVCKFVRCSCPVSNCNYVSSYSNLKSHACSTAHVWGEDDIHFQLVIDRPRI 243

Query: 198 VDLNLD-DKFLVFREKRAEILFVLSSRTQPFGKAITVNSIG-LASNKEGLSLELVMKTKE 257
            ++NL   K +VF+E++   L V+ +     G  +TVN I  +A     LS  L    + 
Sbjct: 244 FNMNLGRKKTVVFKEEKEGDLIVVQAFKGLEGVYVTVNRIAHMAPGIRDLSCSLAKLNEY 303

Query: 258 TTLSLQSGMKGIKELVDELPPEEFLM-IPNKFFGSD 286
           +TL   S +K I+++ +++  E+ LM IP K    D
Sbjct: 304 STLRSGSLVKKIQKVREKMHLEDDLMWIPPKMLSGD 331

BLAST of Tan0013732 vs. TAIR 10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 159.5 bits (402), Expect = 4.3e-39
Identity = 86/237 (36.29%), Postives = 135/237 (56.96%), Query Frame = 0

Query: 48  GTKRKIESQNVTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRI 107
           G KR  ++++  L D ++LDC IC + L IPVFQC NGH+ACS CC K   +CP C   +
Sbjct: 25  GRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPV 84

Query: 108 GDIRCRAIENVLESLKGACQNSKHGCKSKLTFDQRKEHWNTCSFVPCPCPLSDCNFVASA 167
           G IRCRA+E VLES+   C+ +  GC   + + +   H   C+F PC CP+  CN+  S 
Sbjct: 85  GHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSY 144

Query: 168 KKLYAHFNQKHP--SSAINFQYYSSFVVDLNLDDKFLVFREKRAEILFVLSSRTQPFGKA 227
           K LY H++  H   S+A +F   S     + + DK L+ R    ++LFV+    +P G  
Sbjct: 145 KDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQCFEEPCGVY 204

Query: 228 ITVNSIGLASNKEG-LSLELVMKTKE-TTLSLQS-GMKGIKELVDELPPEEFLMIPN 280
           ++V+ I  ++ + G  S  L+  T E  T++ QS  +K + ++  + P + F++IP+
Sbjct: 205 VSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIPH 261

BLAST of Tan0013732 vs. TAIR 10
Match: AT1G66650.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 157.1 bits (396), Expect = 2.1e-38
Identity = 102/306 (33.33%), Postives = 158/306 (51.63%), Query Frame = 0

Query: 5   RISFRKSMATRIVQNDQEAQSEETE------EENAAVNTPEPKTSVKTTGTKRKIES-QN 64
           R S ++   T +  +D     EE +      +++ +    +P   V  T  KR++ S ++
Sbjct: 16  RSSTKRQRRTSVSVDDPSPGEEEEKTLVVLTDDSDSEEDDKPLGDVLRTCRKRRVSSPKS 75

Query: 65  VTLIDPEVLDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKRIGDIRCRAIEN 124
           VTL +  VL+C  C +PL  P+FQC NGH+AC  CC K  + C FC   IGD+RCRA+E 
Sbjct: 76  VTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRCRAMEK 135

Query: 125 VLESLKGACQNSKHGCKSKLTF-DQRKEHWNTCSFVPCPCPLSDCNFVASAKKLYAHFNQ 184
           V+++   +C N+ +GCK   T+ +Q + H   C F PC CP+ DCN++   K L  HF  
Sbjct: 136 VIKAGLVSCSNAIYGCKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIGFYKDLINHFRA 195

Query: 185 KH---PSSAINFQYYSSFVVDLNLD--DKFLVFREKRAEILFVLSSRTQPFGKAITVNSI 244
            H   P    +F +    +  L+LD  DK ++F E++   LFV+       G   TV+ I
Sbjct: 196 THKVSPGDINSFVFDRPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGFIGSHGVYATVSHI 255

Query: 245 G-LASNKEGLSLELVMKTKETTLSLQSGMKGIKEL--VDELPPEEFLMIPNKFFGSDKQI 295
             +       S  L      +TL L   +K I++L   +E P E+FL+IP+     D  +
Sbjct: 256 APMVPEVRKFSCSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIPSYMVNGD-HM 315