Tan0013679 (gene) Snake gourd v1

Overview
NameTan0013679
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionRING-type domain-containing protein
LocationLG07: 66545111 .. 66547748 (-)
RNA-Seq ExpressionTan0013679
SyntenyTan0013679
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAACAAAATCCCCCCTCCTTTTCTGACCCTTAAATTCTAAACAACCTCCTCTCTTTTTTTTCCCTCTCTATAAGCAAACCAAGAAGCTTGCTTTCTTTTATTTTCTTTCTTGAAATTTTAGCTTGAAGTTGTGTTTAATCTTTATAAATATCTTTTTTTTTTTGTTTTTGTTTTTTGATGGGTCTGCCTCAAACTCCAGCCCCTCCTCATCTTTATCCTCAAGCCCTTCAACTTAAGCTTTATCAGGCCTTCATATTTTCCATTCCCATCTTATTTTCCATTATTCTGTTCCTTCTTTTTTACTTGTTTTATCTCAAGAGAAGGGCTTCTTCTCTTTCTTCTTCTTCCTCTTCTTCTTCTTCTTCTAATCCCCCAATTCTTCCAACTACTTCAAATAGCCTCACTCTCTATGTTACAACTGTAAGCAAAACTCCTCTGTTTTTTTCTATCTTCTAAATTTCCATTGCTGAATTTTACTTTTGGGGTCAGAGAATCTATGGAAAGTTTGAAGCTTTAATGTTCATCTTTCCCTTTTTTTTTTTTTGGGAGTGGGAGTTTGATTTGCAGTTGAATTGGTCTGATTTTGTGCCATTTTTCTTAATTTTGTTGATCTTGATTTTACAGGCTTGTCAAGTGGATTTGAAGGTAGACAATCCCATAGACAAACTTCCCAAGATCCTTTTCAATGAAGAGCTAAGGAACAGAGATTCTCTGTAAGTATCATATCACCATTTTCTGATTGTTAAAACTCTCTGTTGGCTTTTTGAGTTTCTTTTAAAAGCAAAAAGGAGAGGTTCATGAACAGTGGTTCCTCTAAATGATTTTGGGATATTGAATTTACTCAAGTTGTCTTTGATCCCTGTGCCACCCACATGCACAAATAGATATAGACTACATGAATCGAAAGAATTCATTTTTTTTCATTGAATCATTTAGGGGTTGTTGGGGGGCGTTAAATAGATTATAATAACTGGGATTATAATAGTATGGGGTTATTATAGTCTGTGTTTGGAGTGTAGAGTATTTCACAAGTTATTATAATGGCCAAACAGACCCTTAGGCATTCATGTGTTCCGGTATCTTTTCAATTTAGTCCTATTGTTTAAAAAGTTTCAACTCAATCTTTTATAATTAGTATTTTTCAATTTAGTCTCTTATTATTGTTGAATGTTAACATTAGTTGTTATAAAGACAAAATAATCTATACTCAGTAAATTGATAGAAATTTAGAGGAGCAAAAAGGTCATTAAAGAGAAGAAAACTTTCATCTTTTAAGTGACTTTTTTTTTCTTCTAAAATTTGTTTATCATTATAAAGGACTAAATTAAAAATTTTCAAACAATAGGGATTAAATGTGAAAAAGCGCTCAAACATCATAGACTAAAACACATATTTACGATAGAAACCATCAGGTGCACCGAGATATCTCCATTAGAATCATATAAAATTAACATGGCTCCTCATCTTGTCCAGTCCACTCTCAATGTGTTACCTCTACATTGCACAAGGAGTTGAAAAAGGCAATATATATATATATACATACACACACAAACAGAACAGTCAAGAGATAGAGATGGAAGGTTTAGTGACTTAAATAGGGAGCACACATAACAAACAATGTTAAGCTAAAAGTGAAGCTCATCTTTTTCAATTACTTTTATTTTTATATACATTATTATTATTATTATTATTATTATTATTATTATTATTTTCTTTTAGGGATTGGAATCTCTTATAACATCCGCTCTTTAATCTTTCAAAAGTGGCTTTTATATTTTTGGTTCTAAAGTTGCTTTTGTTTTTGTTTTCTTTTCTTGAAAGGTGTTATTTGTAGAACCTTAGGCCGACAAAATTGTTACTGAAGTTGAAGGGCATGATTGATTTTTCTTTTTTTTTTCTTTTTAAAGTAAATATCTTCCAATTTTAGTGTTTTTTTTTTATTCTTTTCTTAATTTAGTACATAGTTTGAAACAATAATTGAAAATCCCATTTTTTAAACACAACTTCCAAGAAAGCCTAAGCTTATTGTTTTTTAAAACCTCATTAACGATTAACCTTTTTCTATATGAATCTAAATTTTTCGTTGCTTTTGTCTCTTGTTGGTGTCTCAAAGAGAAAACGAATAGAAATTATAAAATAGTTGACCTAAGCATAACTCCAGTAACTAAGTATCCTTATAAGTTCATAGGTTCAAATCGCTTCCTTATTATCTCGCCTGTAATAATCTCTCTAATTTGGATTGAGAGGAAGATAAAGATTTTAAGTCAACTTCAACCTCATATGGTCCTTAGCAAATCAACAGTAAAACAAAAGAAAAGGCAATAACTTTCTTATCCATTTAACAGGTGCTGCGTCTGCTTGGGAGAATTCGAGATAAAAGAAGAATTGTTGCAAGTCCCATCATGTAAACATGTGTTTCATATTGACTGCATGAATCATTGGCTCACCTCCAACTCAACTTGCCCTCTCTGTAGATGCTCAGTTATCCCAACAACCCGATGCTCTGATCCGCCCATTGTTGCAGCGCCACCGCTAACGCCGCCACCGGCTGCCTCATCGGAACTTCCCCTGACTTCCAACCAGACTGCAGAGACGGTATCATATTCACTCCAACATCCGCAGCTGCAACAACAATAG

mRNA sequence

TTAACAAAATCCCCCCTCCTTTTCTGACCCTTAAATTCTAAACAACCTCCTCTCTTTTTTTTCCCTCTCTATAAGCAAACCAAGAAGCTTGCTTTCTTTTATTTTCTTTCTTGAAATTTTAGCTTGAAGTTGTGTTTAATCTTTATAAATATCTTTTTTTTTTTGTTTTTGTTTTTTGATGGGTCTGCCTCAAACTCCAGCCCCTCCTCATCTTTATCCTCAAGCCCTTCAACTTAAGCTTTATCAGGCCTTCATATTTTCCATTCCCATCTTATTTTCCATTATTCTGTTCCTTCTTTTTTACTTGTTTTATCTCAAGAGAAGGGCTTCTTCTCTTTCTTCTTCTTCCTCTTCTTCTTCTTCTTCTAATCCCCCAATTCTTCCAACTACTTCAAATAGCCTCACTCTCTATGTTACAACTGCTTGTCAAGTGGATTTGAAGGTAGACAATCCCATAGACAAACTTCCCAAGATCCTTTTCAATGAAGAGCTAAGGAACAGAGATTCTCTGTGCTGCGTCTGCTTGGGAGAATTCGAGATAAAAGAAGAATTGTTGCAAGTCCCATCATGTAAACATGTGTTTCATATTGACTGCATGAATCATTGGCTCACCTCCAACTCAACTTGCCCTCTCTGTAGATGCTCAGTTATCCCAACAACCCGATGCTCTGATCCGCCCATTGTTGCAGCGCCACCGCTAACGCCGCCACCGGCTGCCTCATCGGAACTTCCCCTGACTTCCAACCAGACTGCAGAGACGGTATCATATTCACTCCAACATCCGCAGCTGCAACAACAATAG

Coding sequence (CDS)

ATGGGTCTGCCTCAAACTCCAGCCCCTCCTCATCTTTATCCTCAAGCCCTTCAACTTAAGCTTTATCAGGCCTTCATATTTTCCATTCCCATCTTATTTTCCATTATTCTGTTCCTTCTTTTTTACTTGTTTTATCTCAAGAGAAGGGCTTCTTCTCTTTCTTCTTCTTCCTCTTCTTCTTCTTCTTCTAATCCCCCAATTCTTCCAACTACTTCAAATAGCCTCACTCTCTATGTTACAACTGCTTGTCAAGTGGATTTGAAGGTAGACAATCCCATAGACAAACTTCCCAAGATCCTTTTCAATGAAGAGCTAAGGAACAGAGATTCTCTGTGCTGCGTCTGCTTGGGAGAATTCGAGATAAAAGAAGAATTGTTGCAAGTCCCATCATGTAAACATGTGTTTCATATTGACTGCATGAATCATTGGCTCACCTCCAACTCAACTTGCCCTCTCTGTAGATGCTCAGTTATCCCAACAACCCGATGCTCTGATCCGCCCATTGTTGCAGCGCCACCGCTAACGCCGCCACCGGCTGCCTCATCGGAACTTCCCCTGACTTCCAACCAGACTGCAGAGACGGTATCATATTCACTCCAACATCCGCAGCTGCAACAACAATAG

Protein sequence

MGLPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSSNPPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPPPAASSELPLTSNQTAETVSYSLQHPQLQQQ
Homology
BLAST of Tan0013679 vs. ExPASy Swiss-Prot
Match: Q84TF5 (Probable E3 ubiquitin-protein ligase RHA4A OS=Arabidopsis thaliana OX=3702 GN=RHA4A PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.0e-48
Identity = 107/163 (65.64%), Postives = 130/163 (79.75%), Query Frame = 0

Query: 4   PQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSS 63
           PQ+P  PHLYPQA+QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSS S      
Sbjct: 4   PQSPITPHLYPQAIQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSPS------ 63

Query: 64  NPPILPTTSNSLT-LYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEFEIK 123
            P ILP +S+  T  ++ + C +D+KV+   DKL  +LFNEEL  RDSLCCVCLGEFE+K
Sbjct: 64  -PMILPVSSSHQTSSHLPSVCLLDVKVELK-DKLHVVLFNEELGTRDSLCCVCLGEFELK 123

Query: 124 EELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSV-IPTTRCS 165
           EEL+++P CKH+FH+DC++ WL S++TCPLCR SV I +T+ S
Sbjct: 124 EELVEMPLCKHIFHLDCIHLWLYSHNTCPLCRSSVSISSTKTS 158

BLAST of Tan0013679 vs. ExPASy Swiss-Prot
Match: Q570X5 (RING-H2 finger protein ATL58 OS=Arabidopsis thaliana OX=3702 GN=ATL58 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.8e-23
Identity = 70/171 (40.94%), Postives = 103/171 (60.23%), Query Frame = 0

Query: 18  QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSSNPPILPTTSNSLTL 77
           +LKLYQAFIFS+PI F+ I+  LFYL YL+R +S LSS    ++      +P   NSL+ 
Sbjct: 19  ELKLYQAFIFSVPICFTFIILFLFYLIYLRRSSSDLSSLGMRTT-----FIP--GNSLS- 78

Query: 78  YVTTACQVDLKVDNPI-DKLPKILFNEELRNRDSLCCVCLGEFEIKEELLQVPSCKHVFH 137
                  ++L +   + + LP ++F E     DS C VCLG+++  ++L Q+P CKH FH
Sbjct: 79  ------TIELGLSKELREMLPIVVFKESFTVMDSQCSVCLGDYQPNDKLQQIPVCKHTFH 138

Query: 138 IDCMNHWLTSNSTCPLCRCSVIP--TTRCSDPPIVAAPPLTPPPAASSELP 186
           +DC++ WLTS++TCPLCR ++IP  + +  D P+   P L  P    S  P
Sbjct: 139 MDCIDLWLTSHTTCPLCRLALIPSRSRQSQDDPV---PSLVSPDEEVSSQP 172

BLAST of Tan0013679 vs. ExPASy Swiss-Prot
Match: Q9SN28 (RING-H2 finger protein ATL7 OS=Arabidopsis thaliana OX=3702 GN=ATL7 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-22
Identity = 65/158 (41.14%), Postives = 100/158 (63.29%), Query Frame = 0

Query: 3   LPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSS 62
           +P+T AP +   ++ +LKLYQAFIFS+PI F+ I+  + Y+ YL RR S+ +   SS   
Sbjct: 11  IPETYAPSN-STESEKLKLYQAFIFSVPICFTFIVLFVLYVIYL-RRNSTTNVDWSSLGM 70

Query: 63  SNPPILPTTSNSLTLYVTTACQVDLKVDNPI-DKLPKILFNEELRNRDSLCCVCLGEFEI 122
                +PT +N  T         +L +   I + LP +++ E    +DS C VCLG+++ 
Sbjct: 71  RGGTFVPTNNNLST--------AELGLSKDIREMLPVVIYKESFIVKDSQCSVCLGDYQA 130

Query: 123 KEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIP 160
           +E+L Q+PSC H FH++C++ WLTS++TCPLCR S+IP
Sbjct: 131 EEKLQQMPSCGHTFHMECIDLWLTSHTTCPLCRLSLIP 158

BLAST of Tan0013679 vs. ExPASy Swiss-Prot
Match: Q9SN27 (E3 ubiquitin-protein ligase ATL59 OS=Arabidopsis thaliana OX=3702 GN=ATL59 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 3.2e-14
Identity = 48/136 (35.29%), Postives = 81/136 (59.56%), Query Frame = 0

Query: 25  FIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSSNPPILPTTSNSLTLYVTTACQ 84
           F F + +   +IL +L  L+ ++R     +S+++   SS    +PT +N  T        
Sbjct: 22  FTFIVCVPICVILIVLLVLYIMRR-----NSNTNVDWSSLGGFVPTNNNLST-------- 81

Query: 85  VDLKVDNPI-DKLPKILFNEELRNRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMNHW 144
            +L +   I + LP +++ E     D+ C VCLG+++ +E+L Q+PSC H FH++C++ W
Sbjct: 82  AELGLSKDIREMLPIVIYKESFTVNDTQCSVCLGDYQAEEKLQQMPSCGHTFHMECIDLW 141

Query: 145 LTSNSTCPLCRCSVIP 160
           LTS++TCPLCR S+IP
Sbjct: 142 LTSHTTCPLCRLSLIP 144

BLAST of Tan0013679 vs. ExPASy Swiss-Prot
Match: Q8GW38 (RING-H2 finger protein ATL47 OS=Arabidopsis thaliana OX=3702 GN=ATL47 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.2e-12
Identity = 56/140 (40.00%), Postives = 78/140 (55.71%), Query Frame = 0

Query: 26  IFSIPILFSIILFL-----LFYLFYLKRRASSLSSSSSSSSSSNPPILPTTSNSLTLYVT 85
           I  I +L S+I F+     L   +YLK++ S+LSSS + S+ +     P  S+S T Y  
Sbjct: 53  ILFIIVLLSVIFFICSILHLLVRYYLKKKRSNLSSSPNESNQN-----PEFSDSDT-YQR 112

Query: 86  TACQV----DLKVDNP-IDKLPKILFNEELRNRDSL-CCVCLGEFEIKEELLQVPSCKHV 145
              Q+    D  +D   ID LP  L+ E    ++   C VCL EF   ++L  +P+C H 
Sbjct: 113 QLQQLFHLHDSGLDQALIDALPVFLYKEIKGTKEPFDCAVCLCEFSEDDKLRLLPNCSHA 172

Query: 146 FHIDCMNHWLTSNSTCPLCR 155
           FHIDC++ WL SNSTCPLCR
Sbjct: 173 FHIDCIDTWLLSNSTCPLCR 186

BLAST of Tan0013679 vs. NCBI nr
Match: XP_022928899.1 (probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata])

HSP 1 Score: 360.5 bits (924), Expect = 9.5e-96
Identity = 190/207 (91.79%), Postives = 194/207 (93.72%), Query Frame = 0

Query: 1   MGLPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  SSSNPPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEFE 120
           SS  PPILPTTSN+LTLYVTTACQVDLK DNP+DKLPKILFNEELR RDSLCCVCLGEFE
Sbjct: 61  SSF-PPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFE 120

Query: 121 IKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPPPAA 180
           IKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRC DPP+V +PP  PPPA 
Sbjct: 121 IKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDPPVVVSPP-PPPPAG 180

Query: 181 SSELPLTSNQTAETVSYSLQHPQLQQQ 208
           SSELPLTSNQTAE  SYSL HPQLQQQ
Sbjct: 181 SSELPLTSNQTAEMRSYSLLHPQLQQQ 205

BLAST of Tan0013679 vs. NCBI nr
Match: KAG6579565.1 (30S ribosomal protein S6 alpha, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 354.4 bits (908), Expect = 6.8e-94
Identity = 187/204 (91.67%), Postives = 191/204 (93.63%), Query Frame = 0

Query: 4   PQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSS 63
           PQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSS 
Sbjct: 192 PQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSF 251

Query: 64  NPPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEFEIKE 123
            PPILPTTSN+LTLYVTTACQVDLK DNP+DKLPKILFNEELR RDSLCCVCLGEFEIKE
Sbjct: 252 -PPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEIKE 311

Query: 124 ELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPPPAASSE 183
           ELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRC DPP+V +PP  PPPA SSE
Sbjct: 312 ELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDPPVVVSPP-PPPPAGSSE 371

Query: 184 LPLTSNQTAETVSYSLQHPQLQQQ 208
           LP TSNQTAE  SYSL HPQLQQQ
Sbjct: 372 LPPTSNQTAEMRSYSLLHPQLQQQ 393

BLAST of Tan0013679 vs. NCBI nr
Match: XP_023550207.1 (probable E3 ubiquitin-protein ligase RHA4A isoform X1 [Cucurbita pepo subsp. pepo] >XP_023550208.1 probable E3 ubiquitin-protein ligase RHA4A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 351.3 bits (900), Expect = 5.7e-93
Identity = 186/210 (88.57%), Postives = 193/210 (91.90%), Query Frame = 0

Query: 1   MGLPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTPAPPHLYPQALQLKLYQAFIFSIPIL SIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILVSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  SSSN---PPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLG 120
           SSS+   PPILPTTSN+LTLY+TTACQVDLK DNP+DKLPKILFNEELR RDSLCCVCLG
Sbjct: 61  SSSSSSFPPILPTTSNTLTLYITTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLG 120

Query: 121 EFEIKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPP 180
           EFEIKEELLQVPSCKHVFHIDC+NHWLTSNSTCPLCRCSVIPTTRC D P+V +PP  PP
Sbjct: 121 EFEIKEELLQVPSCKHVFHIDCINHWLTSNSTCPLCRCSVIPTTRCFDLPVVVSPP-PPP 180

Query: 181 PAASSELPLTSNQTAETVSYSLQHPQLQQQ 208
           PA SSELP TSNQTAE  SYSL HPQLQQQ
Sbjct: 181 PAGSSELPPTSNQTAEMRSYSLLHPQLQQQ 209

BLAST of Tan0013679 vs. NCBI nr
Match: KAG6605583.1 (putative E3 ubiquitin-protein ligase RHA4A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 349.7 bits (896), Expect = 1.7e-92
Identity = 188/214 (87.85%), Postives = 193/214 (90.19%), Query Frame = 0

Query: 1   MGLPQTP-APPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60
           MG+PQTP APPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS
Sbjct: 1   MGVPQTPAAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60

Query: 61  SSSSNPPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEF 120
           S    PPILPTTSN LTLYVTTAC+VDLK DNPIDKLPKI FNEELR+RDSLCCVCLGEF
Sbjct: 61  SP---PPILPTTSNRLTLYVTTACRVDLKADNPIDKLPKIFFNEELRSRDSLCCVCLGEF 120

Query: 121 EIKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPPP- 180
           EIKEELLQVPSCKHVFHIDCMN WL SNSTCPLCRCSVIPTTRCSDPP++  P LTPPP 
Sbjct: 121 EIKEELLQVPSCKHVFHIDCMNLWLASNSTCPLCRCSVIPTTRCSDPPVLVPPQLTPPPP 180

Query: 181 -----AASSELPLTSNQTAETVSYSLQHPQLQQQ 208
                AAS ELPL SNQTAETVSYSLQHPQLQQQ
Sbjct: 181 PPPPLAASLELPLASNQTAETVSYSLQHPQLQQQ 211

BLAST of Tan0013679 vs. NCBI nr
Match: XP_023534491.1 (probable E3 ubiquitin-protein ligase RHA4A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 349.0 bits (894), Expect = 2.9e-92
Identity = 188/214 (87.85%), Postives = 193/214 (90.19%), Query Frame = 0

Query: 1   MGLPQTP-APPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60
           MG+PQTP APPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS
Sbjct: 1   MGVPQTPAAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60

Query: 61  SSSSNPPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEF 120
           SS   PPILPTTSN LTLYVTTAC+VDLK DNPIDKLPKI FNEELR+RDSLCCVCLGEF
Sbjct: 61  SSP--PPILPTTSNRLTLYVTTACRVDLKADNPIDKLPKIFFNEELRSRDSLCCVCLGEF 120

Query: 121 EIKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPPP- 180
           EIKEELLQVPSCKHVFHIDCMN WL SNSTCPLCRCSVIPTTRCSDPP++  P LTPPP 
Sbjct: 121 EIKEELLQVPSCKHVFHIDCMNLWLASNSTCPLCRCSVIPTTRCSDPPVLVPPQLTPPPP 180

Query: 181 -----AASSELPLTSNQTAETVSYSLQHPQLQQQ 208
                AAS ELPL SNQTAE VSYSLQHPQLQQQ
Sbjct: 181 PPPPLAASLELPLASNQTAEMVSYSLQHPQLQQQ 212

BLAST of Tan0013679 vs. ExPASy TrEMBL
Match: A0A6J1EM64 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC111435669 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.6e-96
Identity = 190/207 (91.79%), Postives = 194/207 (93.72%), Query Frame = 0

Query: 1   MGLPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  SSSNPPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEFE 120
           SS  PPILPTTSN+LTLYVTTACQVDLK DNP+DKLPKILFNEELR RDSLCCVCLGEFE
Sbjct: 61  SSF-PPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFE 120

Query: 121 IKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPPPAA 180
           IKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRC DPP+V +PP  PPPA 
Sbjct: 121 IKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDPPVVVSPP-PPPPAG 180

Query: 181 SSELPLTSNQTAETVSYSLQHPQLQQQ 208
           SSELPLTSNQTAE  SYSL HPQLQQQ
Sbjct: 181 SSELPLTSNQTAEMRSYSLLHPQLQQQ 205

BLAST of Tan0013679 vs. ExPASy TrEMBL
Match: A0A6J1I2V6 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111469090 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 9.0e-92
Identity = 185/207 (89.37%), Postives = 190/207 (91.79%), Query Frame = 0

Query: 1   MGLPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  SSSNPPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEFE 120
           SS  PPILPTTSN+LTLYVTTACQVDLK DNP+DKLPKILFNEELR RDSLCCVCLGEFE
Sbjct: 61  SSF-PPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFE 120

Query: 121 IKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPPPAA 180
           IKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRC D P+V +PP  PP A 
Sbjct: 121 IKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDLPVVVSPP-PPPSAG 180

Query: 181 SSELPLTSNQTAETVSYSLQHPQLQQQ 208
           SSE P TS+QTAE  SYSL HPQLQQQ
Sbjct: 181 SSEPPPTSDQTAEMRSYSLLHPQLQQQ 205

BLAST of Tan0013679 vs. ExPASy TrEMBL
Match: A0A6J1H2A4 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC111459379 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 9.0e-92
Identity = 186/214 (86.92%), Postives = 192/214 (89.72%), Query Frame = 0

Query: 1   MGLPQTP-APPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60
           MG+PQTP APPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS
Sbjct: 1   MGVPQTPAAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60

Query: 61  SSSSNPPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEF 120
           S    PPILPTTSN LTLYVTTAC+VDLK DNP+DKLPKI FNEELR+RDSLCCVCLGEF
Sbjct: 61  SP---PPILPTTSNRLTLYVTTACRVDLKADNPMDKLPKIFFNEELRSRDSLCCVCLGEF 120

Query: 121 EIKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPPP- 180
           EIKEELLQVPSCKHVFHIDCMN WL SNSTCPLCRCSVIPTTRCSDPP++  P LTPPP 
Sbjct: 121 EIKEELLQVPSCKHVFHIDCMNLWLASNSTCPLCRCSVIPTTRCSDPPVLVPPQLTPPPP 180

Query: 181 -----AASSELPLTSNQTAETVSYSLQHPQLQQQ 208
                AAS ELPL SNQTAE VSYSLQHPQLQQQ
Sbjct: 181 PPPPLAASLELPLASNQTAEMVSYSLQHPQLQQQ 211

BLAST of Tan0013679 vs. ExPASy TrEMBL
Match: A0A6J1K5R3 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111490976 PE=4 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 9.9e-91
Identity = 185/212 (87.26%), Postives = 191/212 (90.09%), Query Frame = 0

Query: 1   MGLPQT-PAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60
           MG+PQT  APPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS
Sbjct: 1   MGVPQTSAAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60

Query: 61  SSSSNPPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEF 120
           S    PPILPTTSN LTLYVTTAC+VDLK +NPIDKLPKI FNEELR+RDSLCCVCLGEF
Sbjct: 61  SPP--PPILPTTSNRLTLYVTTACRVDLKANNPIDKLPKIFFNEELRSRDSLCCVCLGEF 120

Query: 121 EIKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPPP- 180
           EIKEELLQVPSCKHVFHIDCMN WL SNSTCPLCRCSVIPTTRCSDPP++  P LTPPP 
Sbjct: 121 EIKEELLQVPSCKHVFHIDCMNLWLASNSTCPLCRCSVIPTTRCSDPPVLVPPQLTPPPP 180

Query: 181 ---AASSELPLTSNQTAETVSYSLQHPQLQQQ 208
              AAS ELPL SNQTAE VSYSLQHPQLQQQ
Sbjct: 181 PPLAASLELPLASNQTAEMVSYSLQHPQLQQQ 210

BLAST of Tan0013679 vs. ExPASy TrEMBL
Match: A0A6J1DWP0 (probable E3 ubiquitin-protein ligase RHA4A OS=Momordica charantia OX=3673 GN=LOC111023829 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 8.4e-90
Identity = 180/207 (86.96%), Postives = 186/207 (89.86%), Query Frame = 0

Query: 1   MGLPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MGLPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLS SSSSS
Sbjct: 1   MGLPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSFSSSSS 60

Query: 61  SSSNPPILPTTSNSLTLYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEFE 120
               PPILPT+SN+LTLYVTTACQVD K DNPI+KLPKILFNEELR RDSLCCVCLGEFE
Sbjct: 61  ----PPILPTSSNALTLYVTTACQVDSKADNPIEKLPKILFNEELRARDSLCCVCLGEFE 120

Query: 121 IKEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCSDPPIVAAPPLTPPPAA 180
           IKEELLQVPSCKHVFHIDCMNHWLT+NSTCPLCRCSVIP  RC DPP+ A  P  P P  
Sbjct: 121 IKEELLQVPSCKHVFHIDCMNHWLTTNSTCPLCRCSVIPPIRCPDPPVAAPSPTLPLP-- 180

Query: 181 SSELPLTSNQTAETVSYSLQHPQLQQQ 208
            SELPLTSNQTAE VSYSL+HPQLQQQ
Sbjct: 181 -SELPLTSNQTAEMVSYSLRHPQLQQQ 200

BLAST of Tan0013679 vs. TAIR 10
Match: AT4G24015.1 (RING/U-box superfamily protein )

HSP 1 Score: 193.7 bits (491), Expect = 1.4e-49
Identity = 107/163 (65.64%), Postives = 130/163 (79.75%), Query Frame = 0

Query: 4   PQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSS 63
           PQ+P  PHLYPQA+QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSS S      
Sbjct: 4   PQSPITPHLYPQAIQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSPS------ 63

Query: 64  NPPILPTTSNSLT-LYVTTACQVDLKVDNPIDKLPKILFNEELRNRDSLCCVCLGEFEIK 123
            P ILP +S+  T  ++ + C +D+KV+   DKL  +LFNEEL  RDSLCCVCLGEFE+K
Sbjct: 64  -PMILPVSSSHQTSSHLPSVCLLDVKVELK-DKLHVVLFNEELGTRDSLCCVCLGEFELK 123

Query: 124 EELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSV-IPTTRCS 165
           EEL+++P CKH+FH+DC++ WL S++TCPLCR SV I +T+ S
Sbjct: 124 EELVEMPLCKHIFHLDCIHLWLYSHNTCPLCRSSVSISSTKTS 158

BLAST of Tan0013679 vs. TAIR 10
Match: AT1G33480.1 (RING/U-box superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.7e-24
Identity = 70/171 (40.94%), Postives = 103/171 (60.23%), Query Frame = 0

Query: 18  QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSSNPPILPTTSNSLTL 77
           +LKLYQAFIFS+PI F+ I+  LFYL YL+R +S LSS    ++      +P   NSL+ 
Sbjct: 19  ELKLYQAFIFSVPICFTFIILFLFYLIYLRRSSSDLSSLGMRTT-----FIP--GNSLS- 78

Query: 78  YVTTACQVDLKVDNPI-DKLPKILFNEELRNRDSLCCVCLGEFEIKEELLQVPSCKHVFH 137
                  ++L +   + + LP ++F E     DS C VCLG+++  ++L Q+P CKH FH
Sbjct: 79  ------TIELGLSKELREMLPIVVFKESFTVMDSQCSVCLGDYQPNDKLQQIPVCKHTFH 138

Query: 138 IDCMNHWLTSNSTCPLCRCSVIP--TTRCSDPPIVAAPPLTPPPAASSELP 186
           +DC++ WLTS++TCPLCR ++IP  + +  D P+   P L  P    S  P
Sbjct: 139 MDCIDLWLTSHTTCPLCRLALIPSRSRQSQDDPV---PSLVSPDEEVSSQP 172

BLAST of Tan0013679 vs. TAIR 10
Match: AT4G10150.1 (RING/U-box superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 7.9e-24
Identity = 65/158 (41.14%), Postives = 100/158 (63.29%), Query Frame = 0

Query: 3   LPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSS 62
           +P+T AP +   ++ +LKLYQAFIFS+PI F+ I+  + Y+ YL RR S+ +   SS   
Sbjct: 11  IPETYAPSN-STESEKLKLYQAFIFSVPICFTFIVLFVLYVIYL-RRNSTTNVDWSSLGM 70

Query: 63  SNPPILPTTSNSLTLYVTTACQVDLKVDNPI-DKLPKILFNEELRNRDSLCCVCLGEFEI 122
                +PT +N  T         +L +   I + LP +++ E    +DS C VCLG+++ 
Sbjct: 71  RGGTFVPTNNNLST--------AELGLSKDIREMLPVVIYKESFIVKDSQCSVCLGDYQA 130

Query: 123 KEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIP 160
           +E+L Q+PSC H FH++C++ WLTS++TCPLCR S+IP
Sbjct: 131 EEKLQQMPSCGHTFHMECIDLWLTSHTTCPLCRLSLIP 158

BLAST of Tan0013679 vs. TAIR 10
Match: AT4G10160.1 (RING/U-box superfamily protein )

HSP 1 Score: 80.1 bits (196), Expect = 2.3e-15
Identity = 48/136 (35.29%), Postives = 81/136 (59.56%), Query Frame = 0

Query: 25  FIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSSNPPILPTTSNSLTLYVTTACQ 84
           F F + +   +IL +L  L+ ++R     +S+++   SS    +PT +N  T        
Sbjct: 22  FTFIVCVPICVILIVLLVLYIMRR-----NSNTNVDWSSLGGFVPTNNNLST-------- 81

Query: 85  VDLKVDNPI-DKLPKILFNEELRNRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMNHW 144
            +L +   I + LP +++ E     D+ C VCLG+++ +E+L Q+PSC H FH++C++ W
Sbjct: 82  AELGLSKDIREMLPIVIYKESFTVNDTQCSVCLGDYQAEEKLQQMPSCGHTFHMECIDLW 141

Query: 145 LTSNSTCPLCRCSVIP 160
           LTS++TCPLCR S+IP
Sbjct: 142 LTSHTTCPLCRLSLIP 144

BLAST of Tan0013679 vs. TAIR 10
Match: AT1G23980.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 3.7e-13
Identity = 56/140 (40.00%), Postives = 78/140 (55.71%), Query Frame = 0

Query: 26  IFSIPILFSIILFL-----LFYLFYLKRRASSLSSSSSSSSSSNPPILPTTSNSLTLYVT 85
           I  I +L S+I F+     L   +YLK++ S+LSSS + S+ +     P  S+S T Y  
Sbjct: 53  ILFIIVLLSVIFFICSILHLLVRYYLKKKRSNLSSSPNESNQN-----PEFSDSDT-YQR 112

Query: 86  TACQV----DLKVDNP-IDKLPKILFNEELRNRDSL-CCVCLGEFEIKEELLQVPSCKHV 145
              Q+    D  +D   ID LP  L+ E    ++   C VCL EF   ++L  +P+C H 
Sbjct: 113 QLQQLFHLHDSGLDQALIDALPVFLYKEIKGTKEPFDCAVCLCEFSEDDKLRLLPNCSHA 172

Query: 146 FHIDCMNHWLTSNSTCPLCR 155
           FHIDC++ WL SNSTCPLCR
Sbjct: 173 FHIDCIDTWLLSNSTCPLCR 186

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84TF52.0e-4865.64Probable E3 ubiquitin-protein ligase RHA4A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q570X53.8e-2340.94RING-H2 finger protein ATL58 OS=Arabidopsis thaliana OX=3702 GN=ATL58 PE=2 SV=1[more]
Q9SN281.1e-2241.14RING-H2 finger protein ATL7 OS=Arabidopsis thaliana OX=3702 GN=ATL7 PE=2 SV=1[more]
Q9SN273.2e-1435.29E3 ubiquitin-protein ligase ATL59 OS=Arabidopsis thaliana OX=3702 GN=ATL59 PE=1 ... [more]
Q8GW385.2e-1240.00RING-H2 finger protein ATL47 OS=Arabidopsis thaliana OX=3702 GN=ATL47 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022928899.19.5e-9691.79probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata][more]
KAG6579565.16.8e-9491.6730S ribosomal protein S6 alpha, chloroplastic, partial [Cucurbita argyrosperma s... [more]
XP_023550207.15.7e-9388.57probable E3 ubiquitin-protein ligase RHA4A isoform X1 [Cucurbita pepo subsp. pep... [more]
KAG6605583.11.7e-9287.85putative E3 ubiquitin-protein ligase RHA4A, partial [Cucurbita argyrosperma subs... [more]
XP_023534491.12.9e-9287.85probable E3 ubiquitin-protein ligase RHA4A [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1EM644.6e-9691.79probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1I2V69.0e-9289.37probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1H2A49.0e-9286.92probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1K5R39.9e-9187.26probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1DWP08.4e-9086.96probable E3 ubiquitin-protein ligase RHA4A OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G24015.11.4e-4965.64RING/U-box superfamily protein [more]
AT1G33480.12.7e-2440.94RING/U-box superfamily protein [more]
AT4G10150.17.9e-2441.14RING/U-box superfamily protein [more]
AT4G10160.12.3e-1535.29RING/U-box superfamily protein [more]
AT1G23980.13.7e-1340.00RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 112..153
e-value: 0.0011
score: 28.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 112..154
e-value: 4.1E-12
score: 46.2
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 112..154
score: 12.017182
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 80..159
e-value: 4.8E-17
score: 63.4
NoneNo IPR availablePANTHERPTHR22765RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAININGcoord: 5..173
NoneNo IPR availablePANTHERPTHR22765:SF187E3 UBIQUITIN-PROTEIN LIGASE RHA4A-RELATEDcoord: 5..173
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 92..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0013679.1Tan0013679.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity