Tan0013576 (gene) Snake gourd v1

BLAST of Tan0013576 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 3.1e-288
Identity = 489/737 (66.35%), Postives = 591/737 (80.19%), Query Frame = 0

Query: 39  TYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEK 98
           TYI+HM ++ MP +FD H  WYDSSL+S+SDSA++LY+Y   +HGFSTRLT EEA  +  
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYENAIHGFSTRLTQEEADSLMT 90

Query: 99  QEGILAVIPEVKYELHTTRTPEFLGLGK-SVSFFPASEKVGEVIVGVLDTGVWPELESFN 158
           Q G+++V+PE +YELHTTRTP FLGL + +   FP +    +V+VGVLDTGVWPE +S++
Sbjct: 91  QPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYS 150

Query: 159 DAGLGPVPPTWKGECEVGKNFSSSNCNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDD 218
           D G GP+P +WKG CE G NF++S CNRKLIGAR+F++GYE+  G IDES+ES+SPRDDD
Sbjct: 151 DEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDD 210

Query: 219 GHGTHTSTTAAGSAVTGASLFGFAAGTARGMAAEARVATYKVCWLGGCFGSDILAAIDKA 278
           GHGTHTS+TAAGS V GASL G+A+GTARGMA  ARVA YKVCWLGGCF SDILAAIDKA
Sbjct: 211 GHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKA 270

Query: 279 VEDGVNVLSLSLGGSSPDYFRDNVAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWIT 338
           + D VNVLS+SLGG   DY+RD VAIG+F+A  +G+ VSCSAGN GPSS SLSNVAPWIT
Sbjct: 271 IADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWIT 330

Query: 339 TVGAGTLDRDFPAYVTLGNGKKFTGESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTST 398
           TVGAGTLDRDFPA   LGNGK FTG SL+ G+ LPD L+P +YA NASN+T+G+LC+T T
Sbjct: 331 TVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGT 390

Query: 399 LNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVG 458
           L P KV GKIV+CDRG N+RVQKG VVK AGG GMILANT   GEE +ADAHLLPA  VG
Sbjct: 391 LIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVG 450

Query: 459 QKAGDVIKNYISSDANPTATISSGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPG 518
           +KAGD+I++Y+++D NPTA+IS   T +GV+PSPVVAAFSSRGPN +TP ILKPDLIAPG
Sbjct: 451 EKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPG 510

Query: 519 VNILAGWTGGAGPTGSDSDKRRVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSA 578
           VNILA WTG AGPTG  SD RRV FNIISGTSMSCPH+SGLAALLK+ HP+WSPAAIRSA
Sbjct: 511 VNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSA 570

Query: 579 LMTTAYSTYKNGEALKDVSNGLPTTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCAL 638
           LMTTAY TYK+G+ L D++ G P+TPFD GAGHV+PT A +PGL+YD TT+DY  FLCAL
Sbjct: 571 LMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCAL 630

Query: 639 NYSSLQIKIIAKKDFTCSGNKNYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLT 698
           NY+S QI+ ++++++TC  +K+Y + DLNYPSFAV ++     G G       KYTRT+T
Sbjct: 631 NYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVD-----GVG-----AYKYTRTVT 690

Query: 699 NKGAPSTYKASVTSKSPSVKIVVEPESLSFGEANEQKSFTVTF-VASPMPSGSESFARLE 758
           + G   TY   VTS++  VKI VEP  L+F EANE+KS+TVTF V S  PSGS SF  +E
Sbjct: 691 SVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIE 750

Query: 759 WSDGKHIVGSPIAFTWT 774
           WSDGKH+VGSP+A +WT
Sbjct: 751 WSDGKHVVGSPVAISWT 757

BLAST of Tan0013576 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 2.8e-220
Identity = 410/740 (55.41%), Postives = 505/740 (68.24%), Query Frame = 0

Query: 37  KKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEA-KL 96
           KKTYII ++ ++ P++F  H  WY S L S S    +LY+Y T  HGFS  L   EA  L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 97  IEKQEGILAVIPEVKYELHTTRTPEFLGLGKSVSFFPASEKVGEVIVGVLDTGVWPELES 156
           +     IL +  +  Y LHTTRTPEFLGL               VI+GVLDTGVWPE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 157 FNDAGLGPVPPTWKGECEVGKNFSSSNCNRKLIGARYFSKGYE-AAFGTIDESQESKSPR 216
           F+D  +  +P  WKGECE G +F S  CN+KLIGAR FSKG++ A+ G     +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 217 DDDGHGTHTSTTAAGSAVTGASLFGFAAGTARGMAAEARVATYKVCWLGGCFGSDILAAI 276
           D DGHGTHTSTTAAGSAV  AS  G+AAGTARGMA  ARVATYKVCW  GCFGSDILAA+
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 277 DKAVEDGVNVLSLSLGGSSPDYFRDNVAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAP 336
           D+A+ DGV+VLSLSLGG S  Y+RD +AIG+FSA  +GVFVSCSAGN GP+  S++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 337 WITTVGAGTLDRDFPAYVTLGNGKKFTGESLYSGKPLPDSLIPIVYAANASNSTSGSLCL 396
           W+ TVGAGTLDRDFPA+  LGNGK+ TG SLYSG  +    + +VY  N  NS+S +LCL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 397 TSTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMILANTETYGEEQLADAHLLPAA 456
             +L+ + V GKIVVCDRG N+RV+KG VV++AGG GMI+ANT   GEE +AD+HLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 457 AVGQKAGDVIKNYISSDANPTATISSGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLI 516
           AVG+K GD+++ Y+ SD+ PTA +    T L V+PSPVVAAFSSRGPN +TP+ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 517 APGVNILAGWTGGAGPTGSDSDKRRVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAI 576
            PGVNILAGW+   GPTG D D RR  FNI+SGTSMSCPHISGLA LLKAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 577 RSALMTTAYSTYKNGEALKDVSNGLPTTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFL 636
           +SALMTTAY        L D ++   + P+  G+GHV+P  AL PGLVYD +T++Y  FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 637 CALNYSSLQIKIIAKKDFTCSGNKNYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTR 696
           C+L+Y+   I  I K+       K      LNYPSF+V         GG      V+YTR
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF-------GG---KRVVRYTR 686

Query: 697 TLTNKGAPSTYKASVTSKSPSVKIVVEPESLSFGEANEQKSFTVTFVASPMPS--GSESF 756
            +TN GA S+      + +PSV I V+P  LSF    E+K +TVTFV+    S      F
Sbjct: 687 EVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEF 746

Query: 757 ARLEWSDGKHIVGSPIAFTW 773
             + WS+ +H V SP+AF+W
Sbjct: 747 GSITWSNPQHEVRSPVAFSW 751

BLAST of Tan0013576 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 3.4e-218
Identity = 406/774 (52.45%), Postives = 518/774 (66.93%), Query Frame = 0

Query: 15  LFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVS------ 74
           LF I   +  F  ++   Q   KKTY+IHMD++ MP  + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 75  ---DSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEFLGLG 134
              ++ ++LY+Y T  HG + +LT EEA+ +E+++G++AVIPE +YELHTTR+P FLGL 
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 135 KSVSFFPASEKV--GEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSSSNC 194
           +  S    +E+V   +V+VGVLDTG+WPE ESFND G+ PVP TW+G CE GK F   NC
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 195 NRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGFAAG 254
           NRK++GAR F +GYEAA G IDE  E KSPRD DGHGTHT+ T AGS V GA+LFGFA G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 255 TARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDNVAI 314
           TARGMA +ARVA YKVCW+GGCF SDIL+A+D+AV DGV VLS+SLGG    Y RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 315 GSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGE 374
            +F A   GVFVSCSAGNGGP   SL+NV+PWITTVGA T+DRDFPA V +G  + F G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 375 SLYSGKPL--PDSLIPIVY-AANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 434
           SLY G+ +   +   P+VY   NAS+    S CL   L+   VAGKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 435 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISS 494
           G VVK AGG GM+L NT T GEE +AD+H+LPA AVG+K G +IK Y  +    TA++  
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 495 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRV 554
             TR+G++PSPVVAAFSSRGPNFL+ +ILKPDL+APGVNILA WTG   P+   SD RRV
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 555 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLP 614
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTTAY      + L D S   P
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 615 TTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAK-KDFTCSGNKN 674
           ++P+D GAGH++P  A DPGLVYD    +YF FLC  + S  Q+K+  K  + TC     
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 675 YRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGAP-STYKASVTSKSPSVKI 734
               +LNYP+ +            +     +   RT+TN G   S+YK SV S      +
Sbjct: 672 KNPGNLNYPAISALFPE-------NTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKGASV 731

Query: 735 VVEPESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTW 773
            V+P++L+F   +++ S+TVTF  +        F  L W    H V SP+  TW
Sbjct: 732 TVQPKTLNFTSKHQKLSYTVTF-RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Tan0013576 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 2.2e-209
Identity = 398/780 (51.03%), Postives = 516/780 (66.15%), Query Frame = 0

Query: 10  SLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSD 69
           SL  + F   +  C F+ S + +  L  ++YI+H+ R++ P  F  H  W+ S L+S+  
Sbjct: 5   SLSSIFFVFPLLLCFFSPSSSSSDGL--ESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPS 64

Query: 70  SAQ---MLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEFLGLGK 129
           S Q   +LYSY+  VHGFS RL+  +   + +   +++VIP+   E+HTT TP FLG  +
Sbjct: 65  SPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQ 124

Query: 130 SVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSSSNCNRK 189
           +   +  S    +VIVGVLDTG+WPE  SF+D+GLGP+P TWKGECE+G +F +S+CNRK
Sbjct: 125 NSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRK 184

Query: 190 LIGARYFSKGY-EAAFGTIDE-SQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGFAAGT 249
           LIGAR F +GY     GT    ++ES+SPRD +GHGTHT++TAAGS V  ASL+ +A GT
Sbjct: 185 LIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGT 244

Query: 250 ARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLG--GSSPDYFRDNVA 309
           A GMA++AR+A YK+CW GGC+ SDILAA+D+AV DGV+V+SLS+G  GS+P+Y  D++A
Sbjct: 245 ATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIA 304

Query: 310 IGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTG 369
           IG+F A   G+ VSCSAGN GP+  + +N+APWI TVGA T+DR+F A    G+GK FTG
Sbjct: 305 IGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTG 364

Query: 370 ESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGV 429
            SLY+G+ LPDS + +VY    S      LC    LN + V GKIV+CDRGGN+RV+KG 
Sbjct: 365 TSLYAGESLPDSQLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGS 424

Query: 430 VVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISSGT 489
            VK AGGAGMILANT   GEE  AD+HL+PA  VG KAGD I++YI +  +PTA IS   
Sbjct: 425 AVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLG 484

Query: 490 TRLGVQ-PSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRVP 549
           T +G   PSP VAAFSSRGPN LTP ILKPD+IAPGVNILAGWTG  GPT  D D RRV 
Sbjct: 485 TLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQ 544

Query: 550 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLPT 609
           FNIISGTSMSCPH+SGLAALL+ AHPDWSPAAI+SAL+TTAY    +GE ++D++ G  +
Sbjct: 545 FNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSS 604

Query: 610 TPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDF---TCSGNK 669
             F  GAGHV+P  AL+PGLVYD    +Y AFLCA+ Y    I +  +       C  +K
Sbjct: 605 NSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSK 664

Query: 670 NYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGAPSTYKASVTSKSP-SVK 729
                DLNYPSF+V   +             VKY R + N G+       V  KSP +V+
Sbjct: 665 LRTAGDLNYPSFSVVFASTG---------EVVKYKRVVKNVGSNVDAVYEVGVKSPANVE 724

Query: 730 IVVEPESLSFGEANEQKSFTVTFVASPMPSG-----SESFARLEWSDGKHIVGSPIAFTW 773
           I V P  L+F +      + VTF +  +  G        F  +EW+DG+H+V SP+A  W
Sbjct: 725 IDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Tan0013576 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 1.4e-200
Identity = 387/781 (49.55%), Postives = 510/781 (65.30%), Query Frame = 0

Query: 10  SLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSD 69
           + +F  FF+   S   + + + N      TYI+H+D    P  F  HF WY SSL S++ 
Sbjct: 2   AFFFYFFFLLTLSSPSSSASSSN----SLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTS 61

Query: 70  S-AQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEFLGLGKS- 129
           S   ++++Y+TV HGFS RLT ++A  +     +++VIPE    LHTTR+PEFLGL  + 
Sbjct: 62  SPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTD 121

Query: 130 -VSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSSSNCNRK 189
                  S+   ++++GV+DTGVWPE  SF+D GLGPVP  WKG+C   ++F  S CNRK
Sbjct: 122 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 181

Query: 190 LIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGFAAGTAR 249
           L+GAR+F  GYEA  G ++E+ E +SPRD DGHGTHT++ +AG  V  AS  G+A G A 
Sbjct: 182 LVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAA 241

Query: 250 GMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDNVAIGSF 309
           GMA +AR+A YKVCW  GC+ SDILAA D AV DGV+V+SLS+GG    Y+ D +AIG+F
Sbjct: 242 GMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAF 301

Query: 310 SAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGESLY 369
            A  +G+FVS SAGNGGP + +++NVAPW+TTVGAGT+DRDFPA V LGNGK  +G S+Y
Sbjct: 302 GAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVY 361

Query: 370 SGKPL-PDSLIPIVYAAN--ASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGVV 429
            G  L P  + P+VY  +    +  S SLCL  +L+P  V GKIV+CDRG NSR  KG +
Sbjct: 362 GGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEI 421

Query: 430 VKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYIS------SDANPTAT 489
           V++ GG GMI+AN    GE  +AD H+LPA +VG   GD I+ YIS      S  +PTAT
Sbjct: 422 VRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTAT 481

Query: 490 ISSGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDK 549
           I    TRLG++P+PVVA+FS+RGPN  TP+ILKPD+IAPG+NILA W    GP+G  SD 
Sbjct: 482 IVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 541

Query: 550 RRVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSN 609
           RR  FNI+SGTSM+CPH+SGLAALLKAAHPDWSPAAIRSAL+TTAY+   +GE + D S 
Sbjct: 542 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDEST 601

Query: 610 GLPTTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGN 669
           G  ++  D G+GHV+PT A+DPGLVYD T+ DY  FLC  NY+   I  I ++   C G 
Sbjct: 602 GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGA 661

Query: 670 KNY-RLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKG-APSTYKASVTSKSPS 729
           +    + +LNYPSF+V  +       G++  +T  + RT+TN G + S Y+  +      
Sbjct: 662 RRAGHVGNLNYPSFSVVFQQ-----YGESKMST-HFIRTVTNVGDSDSVYEIKIRPPR-G 721

Query: 730 VKIVVEPESLSFGEANEQKSF-----TVTFVASPMPSGSESFARLEWSDGKHIVGSPIAF 772
             + VEPE LSF    ++ SF     T     SP  +  E+   + WSDGK  V SP+  
Sbjct: 722 TTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVET-GHIVWSDGKRNVTSPLVV 770

BLAST of Tan0013576 vs. NCBI nr
Match: XP_038885098.1 (subtilisin-like protease SBT1.7 [Benincasa hispida])

HSP 1 Score: 1403.7 bits (3632), Expect = 0.0e+00
Identity = 703/773 (90.94%), Postives = 736/773 (95.21%), Query Frame = 0

Query: 1   MKLQTCRFSSLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60
           MK+QTCR S  WFLLF IS  SCSF  +Q  N+QLKKKTY+IHMDRTNMPQAFDDHF+WY
Sbjct: 1   MKMQTCRLSQ-WFLLFLISFCSCSFTEAQKSNRQLKKKTYVIHMDRTNMPQAFDDHFKWY 60

Query: 61  DSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPE 120
           DSSLKSVS+SAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPE
Sbjct: 61  DSSLKSVSNSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPE 120

Query: 121 FLGLGKSVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSS 180
           FLGLGKSVSFFPASEKV EVI+GVLDTGVWPELESFNDAGLGPVP +WKGECEVGKNF+S
Sbjct: 121 FLGLGKSVSFFPASEKVSEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTS 180

Query: 181 SNCNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGF 240
           S+CNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGASLFGF
Sbjct: 181 SSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGF 240

Query: 241 AAGTARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDN 300
           AAGTARGMAAEARVATYKVCWLGGCF SDILAAIDKAVEDGVNVLSLSLGGSSPDY+RDN
Sbjct: 241 AAGTARGMAAEARVATYKVCWLGGCFSSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDN 300

Query: 301 VAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKF 360
           VAIG+FSAAAQGVFVSCSAGNGGPSSG+LSNVAPWITTVGAGTLDRDFPAYVTLGNGKK 
Sbjct: 301 VAIGAFSAAAQGVFVSCSAGNGGPSSGTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKI 360

Query: 361 TGESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420
           TGESLYSGKPLPDSL+PIVYA NASNSTSGSLCL+STLNPAKVAGKIVVCDRGGNSRVQK
Sbjct: 361 TGESLYSGKPLPDSLLPIVYAGNASNSTSGSLCLSSTLNPAKVAGKIVVCDRGGNSRVQK 420

Query: 421 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISS 480
           G+VVKEAGG GMILANTE YGEEQLADAHL P AAVG+K+GD IK+YISSDANPTATIS+
Sbjct: 421 GLVVKEAGGVGMILANTEAYGEEQLADAHLTPTAAVGKKSGDAIKSYISSDANPTATIST 480

Query: 481 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRV 540
           GTTRLGVQPSPVVAAFSSRGPN LTP ILKPDLIAPGVNILAGWTGGAGPTG DSDKR V
Sbjct: 481 GTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHV 540

Query: 541 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLP 600
            FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGE ++DVSNG P
Sbjct: 541 AFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEVIQDVSNGSP 600

Query: 601 TTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGNKNY 660
           +TPFD+GAGHVNPTAALDPGLVYD TTDDYFAFLCALNYSSLQIK+I+K+DFTC+GNKNY
Sbjct: 601 STPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVISKRDFTCNGNKNY 660

Query: 661 RLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGAPSTYKASVTSKSPSVKIVV 720
           +LEDLNYPSFAVPLETPSTRGG D APTTVKYTRTLTNKGAPSTYK SVT+    VKIVV
Sbjct: 661 KLEDLNYPSFAVPLETPSTRGGEDVAPTTVKYTRTLTNKGAPSTYKVSVTAPK-EVKIVV 720

Query: 721 EPESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 774
           EPESLSF EANEQKS+TVTF+ASPMPSGSESFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 EPESLSFAEANEQKSYTVTFIASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 771

BLAST of Tan0013576 vs. NCBI nr
Match: KAG7029314.1 (Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 698/774 (90.18%), Postives = 735/774 (94.96%), Query Frame = 0

Query: 1   MKLQTCRFSSLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60
           MK+QTCR S  WFLLF IS  SCSFA +Q  +QQLKKKTYIIHMDRTNMPQAFDDHFQWY
Sbjct: 1   MKIQTCRVSQ-WFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60

Query: 61  DSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPE 120
           DSSLKSVSDSAQ LY YNTVVHGFSTRLTVEEAKLIEKQEGILAVIPE+KY+LHTTRTPE
Sbjct: 61  DSSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPE 120

Query: 121 FLGLGKSVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSS 180
           FLGL KSVSFFPAS KVGEVIVGVLDTGV PELESF+D GLGPVP +WKGECEVGKNFSS
Sbjct: 121 FLGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSS 180

Query: 181 SNCNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGF 240
           S+CNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGASLFGF
Sbjct: 181 SSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGF 240

Query: 241 AAGTARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDN 300
           AAGTA+GMAAEARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDY+RDN
Sbjct: 241 AAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDN 300

Query: 301 VAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKF 360
           VAIG+FSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP YVTLGNGKKF
Sbjct: 301 VAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKF 360

Query: 361 TGESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420
           TG+SLY+GKPL DSLIPIVYAA+ASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK
Sbjct: 361 TGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420

Query: 421 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISS 480
           G+VVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQK GD IK+YIS++ANPTATIS+
Sbjct: 421 GLVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISA 480

Query: 481 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRV 540
           GTTRLGVQPSP+VAAFSSRGPN LTPQILKPDLIAPGVNILAGW GG GPTG DSDKR V
Sbjct: 481 GTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHV 540

Query: 541 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLP 600
            FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYK GEA++DVS+GLP
Sbjct: 541 AFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLP 600

Query: 601 TTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGNKNY 660
           +TPFD+GAGHVNPTAALDPGLVYDAT +DYFAFLCALNYSS QIK+I+KKDFTCSGNKNY
Sbjct: 601 STPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNY 660

Query: 661 RLEDLNYPSFAVPLETPSTRGGGD-AAPTTVKYTRTLTNKGAPSTYKASVTSKSPSVKIV 720
           +LEDLNYPSFAV LETPST+GGG+  APTTVKYTRTLTNKGAPSTYK SVTSKSPSVKI+
Sbjct: 661 KLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIM 720

Query: 721 VEPESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 774
           VEPESLSF + NEQKS+TVTFVAS MPSGSESFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 VEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Tan0013576 vs. NCBI nr
Match: XP_022131596.1 (subtilisin-like protease SBT1.7 [Momordica charantia])

HSP 1 Score: 1384.0 bits (3581), Expect = 0.0e+00
Identity = 690/773 (89.26%), Postives = 734/773 (94.95%), Query Frame = 0

Query: 1   MKLQTCRFSSLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60
           MK+QTCR S L FLLF I  +S S+  ++  NQ+LKKKTYIIHMDRTNMPQAFDDHFQWY
Sbjct: 4   MKMQTCRVSRL-FLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWY 63

Query: 61  DSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPE 120
           DSSLKSVSDSAQMLYSY+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KYELHTTRTPE
Sbjct: 64  DSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPE 123

Query: 121 FLGLGKSVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSS 180
           FLGLGKS SFFPAS KVGEVI+G+LDTGVWPELESFNDAGLGPVP +WKGECEVGKNFSS
Sbjct: 124 FLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSS 183

Query: 181 SNCNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGF 240
           S+CNRKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHGTHTSTTAAGSAVTGA+LFG+
Sbjct: 184 SSCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGY 243

Query: 241 AAGTARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDN 300
           A+GTARGMAA+ARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDY+RDN
Sbjct: 244 ASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDN 303

Query: 301 VAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKF 360
           VAIG+FSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAY+TLGNGKKF
Sbjct: 304 VAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKF 363

Query: 361 TGESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420
           TGESLYSGKPLPDSL+PIVYAA+ASNSTSGS CLT TLNPAKVAGKIVVCDRGGNSRVQK
Sbjct: 364 TGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQK 423

Query: 421 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISS 480
           GVVVKEAGGAGMILANTETYGEE+LADAHLLPAAAVGQK GD IK+YISSDANPTA  S+
Sbjct: 424 GVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFST 483

Query: 481 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRV 540
           GTTRLGVQPSPVVAAFSSRGPN LTP ILKPDLIAPGVNILAGWTG AGPTG D DKRRV
Sbjct: 484 GTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRV 543

Query: 541 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLP 600
            FNIISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGEA++D+S+GLP
Sbjct: 544 AFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLP 603

Query: 601 TTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGNKNY 660
           +TPFD+GAGHVNP AALDPGLVYD TTDDYF FLCALNY+SLQIK+IAKKDFTCS +KNY
Sbjct: 604 STPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNY 663

Query: 661 RLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGAPSTYKASVTSKSPSVKIVV 720
           +LEDLNYPSFAVPLETPS RGGGDAAPTTVKYTRTLTNK A STYK S TSKSPSVKIVV
Sbjct: 664 KLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVV 723

Query: 721 EPESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 774
           EPESLSF E NEQKS+TVTF+ASPMPSG+ESFARLEWSDGKHIVGSPIAFTWT
Sbjct: 724 EPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 775

BLAST of Tan0013576 vs. NCBI nr
Match: KAG6598344.1 (Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1381.3 bits (3574), Expect = 0.0e+00
Identity = 697/774 (90.05%), Postives = 734/774 (94.83%), Query Frame = 0

Query: 1   MKLQTCRFSSLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60
           MK+QTCR S  WFLLF IS  SCSFA +Q  +QQLKKKTYIIHMDRTNMPQAFDDHFQWY
Sbjct: 1   MKIQTCRVSQ-WFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60

Query: 61  DSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPE 120
           DSSLKSVSDSAQ LY YNTVVHGFSTRLTVEEAKLIEKQEGILAVIPE+KY+LHTTRTPE
Sbjct: 61  DSSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPE 120

Query: 121 FLGLGKSVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSS 180
           FLGL KSVSFFPAS KVGEVIVGVLDTGV PELESF+D GLGPVP +WKGECEVGKNFSS
Sbjct: 121 FLGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSS 180

Query: 181 SNCNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGF 240
           S+CNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGASLFGF
Sbjct: 181 SSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGF 240

Query: 241 AAGTARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDN 300
           AAGTA+GMAAEARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDY+RDN
Sbjct: 241 AAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDN 300

Query: 301 VAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKF 360
           VAIG+FSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP YVTLGNGKKF
Sbjct: 301 VAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKF 360

Query: 361 TGESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420
           TG+SLY+GKPL DSLIPIVYAA+ASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK
Sbjct: 361 TGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420

Query: 421 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISS 480
           G+VVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQK GD IK+YIS++ANPTATIS+
Sbjct: 421 GLVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISA 480

Query: 481 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRV 540
           GTTRLGVQPSP+VAAFSSRGPN LTPQILKPDLIAPGVNILAGW GG GPTG DSDKR V
Sbjct: 481 GTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHV 540

Query: 541 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLP 600
            FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYK GEA++DVS+GLP
Sbjct: 541 AFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLP 600

Query: 601 TTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGNKNY 660
           +TPFD+GAGHVNPTAALDPGLVYDAT +DYFAFLCALNYSS QIK+I+KKDFTCS NKNY
Sbjct: 601 STPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSRNKNY 660

Query: 661 RLEDLNYPSFAVPLETPSTRGGGD-AAPTTVKYTRTLTNKGAPSTYKASVTSKSPSVKIV 720
           +LEDLNYPSFAV LETPST+GGG+  APTTVKYTRTLTNKGAPSTYK SVTSKSPSVKI+
Sbjct: 661 KLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIM 720

Query: 721 VEPESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 774
           VEPESLSF + NEQKS+TVTFVAS MPSGSESFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 VEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Tan0013576 vs. NCBI nr
Match: XP_022961887.1 (subtilisin-like protease SBT1.7 [Cucurbita moschata])

HSP 1 Score: 1377.5 bits (3564), Expect = 0.0e+00
Identity = 694/774 (89.66%), Postives = 733/774 (94.70%), Query Frame = 0

Query: 1   MKLQTCRFSSLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60
           MK+QTCR S  WFLLF IS  SCSFA +Q  +QQLKKKTYIIHMDRTNMPQAFDDHFQWY
Sbjct: 1   MKIQTCRVSQ-WFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60

Query: 61  DSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPE 120
           DSSLKSVSDSAQ LY YNTVVHGFSTRLTVEEAKLIEKQEGILAVIPE+KY+LHTTRTPE
Sbjct: 61  DSSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPE 120

Query: 121 FLGLGKSVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSS 180
           FLGL KSVSFFPAS KVGEVIVGVLDTGV PELESF+D GLGPVP +WKGECEVGKNFSS
Sbjct: 121 FLGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSS 180

Query: 181 SNCNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGF 240
           S+CNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGASLFGF
Sbjct: 181 SSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGF 240

Query: 241 AAGTARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDN 300
            AGTA+GMAAEARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDY+RDN
Sbjct: 241 GAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDN 300

Query: 301 VAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKF 360
           VAIG+FSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP YVTLGNGKKF
Sbjct: 301 VAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKF 360

Query: 361 TGESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420
           TG+SLY+GKPL DSLIPIVYAA+ASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK
Sbjct: 361 TGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420

Query: 421 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISS 480
           G+VVK+AGGAGMILANTETYGEEQLADAHLLPAAAVGQK GD IK+YIS++ANPTATIS+
Sbjct: 421 GLVVKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISA 480

Query: 481 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRV 540
           G+TRLGVQPSP+VAAFSSRGPN LTPQILKPDLIAPGVNILAGWT G GPTG DSDKR V
Sbjct: 481 GSTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTSGVGPTGLDSDKRHV 540

Query: 541 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLP 600
            FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYK GEA++DVS+GLP
Sbjct: 541 AFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLP 600

Query: 601 TTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGNKNY 660
           +TPFD+GAGHVNPTAALDPGLVYDAT +DYFAFLCALNYSS QIK+I+KKDFTCSGNKNY
Sbjct: 601 STPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNY 660

Query: 661 RLEDLNYPSFAVPLETPSTRGGGD-AAPTTVKYTRTLTNKGAPSTYKASVTSKSPSVKIV 720
           +LEDLNYPSFAV LETPST+GG +  APTTVKYTRTLTNKGAPSTYK SVTSKSPSVKI+
Sbjct: 661 KLEDLNYPSFAVALETPSTKGGAEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIM 720

Query: 721 VEPESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 774
           VEPESLSF + NEQKS+TVTFVAS MPSGSESFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 VEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Tan0013576 vs. ExPASy TrEMBL
Match: A0A6J1BPX8 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111004737 PE=3 SV=1)

HSP 1 Score: 1384.0 bits (3581), Expect = 0.0e+00
Identity = 690/773 (89.26%), Postives = 734/773 (94.95%), Query Frame = 0

Query: 1   MKLQTCRFSSLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60
           MK+QTCR S L FLLF I  +S S+  ++  NQ+LKKKTYIIHMDRTNMPQAFDDHFQWY
Sbjct: 4   MKMQTCRVSRL-FLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWY 63

Query: 61  DSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPE 120
           DSSLKSVSDSAQMLYSY+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KYELHTTRTPE
Sbjct: 64  DSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPE 123

Query: 121 FLGLGKSVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSS 180
           FLGLGKS SFFPAS KVGEVI+G+LDTGVWPELESFNDAGLGPVP +WKGECEVGKNFSS
Sbjct: 124 FLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSS 183

Query: 181 SNCNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGF 240
           S+CNRKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHGTHTSTTAAGSAVTGA+LFG+
Sbjct: 184 SSCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGY 243

Query: 241 AAGTARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDN 300
           A+GTARGMAA+ARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDY+RDN
Sbjct: 244 ASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDN 303

Query: 301 VAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKF 360
           VAIG+FSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAY+TLGNGKKF
Sbjct: 304 VAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKF 363

Query: 361 TGESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420
           TGESLYSGKPLPDSL+PIVYAA+ASNSTSGS CLT TLNPAKVAGKIVVCDRGGNSRVQK
Sbjct: 364 TGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQK 423

Query: 421 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISS 480
           GVVVKEAGGAGMILANTETYGEE+LADAHLLPAAAVGQK GD IK+YISSDANPTA  S+
Sbjct: 424 GVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFST 483

Query: 481 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRV 540
           GTTRLGVQPSPVVAAFSSRGPN LTP ILKPDLIAPGVNILAGWTG AGPTG D DKRRV
Sbjct: 484 GTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRV 543

Query: 541 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLP 600
            FNIISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGEA++D+S+GLP
Sbjct: 544 AFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLP 603

Query: 601 TTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGNKNY 660
           +TPFD+GAGHVNP AALDPGLVYD TTDDYF FLCALNY+SLQIK+IAKKDFTCS +KNY
Sbjct: 604 STPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNY 663

Query: 661 RLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGAPSTYKASVTSKSPSVKIVV 720
           +LEDLNYPSFAVPLETPS RGGGDAAPTTVKYTRTLTNK A STYK S TSKSPSVKIVV
Sbjct: 664 KLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVV 723

Query: 721 EPESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 774
           EPESLSF E NEQKS+TVTF+ASPMPSG+ESFARLEWSDGKHIVGSPIAFTWT
Sbjct: 724 EPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 775

BLAST of Tan0013576 vs. ExPASy TrEMBL
Match: A0A6J1HBB4 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111462526 PE=3 SV=1)

HSP 1 Score: 1377.5 bits (3564), Expect = 0.0e+00
Identity = 694/774 (89.66%), Postives = 733/774 (94.70%), Query Frame = 0

Query: 1   MKLQTCRFSSLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60
           MK+QTCR S  WFLLF IS  SCSFA +Q  +QQLKKKTYIIHMDRTNMPQAFDDHFQWY
Sbjct: 1   MKIQTCRVSQ-WFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWY 60

Query: 61  DSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPE 120
           DSSLKSVSDSAQ LY YNTVVHGFSTRLTVEEAKLIEKQEGILAVIPE+KY+LHTTRTPE
Sbjct: 61  DSSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPE 120

Query: 121 FLGLGKSVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSS 180
           FLGL KSVSFFPAS KVGEVIVGVLDTGV PELESF+D GLGPVP +WKGECEVGKNFSS
Sbjct: 121 FLGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSS 180

Query: 181 SNCNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGF 240
           S+CNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGASLFGF
Sbjct: 181 SSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGF 240

Query: 241 AAGTARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDN 300
            AGTA+GMAAEARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDY+RDN
Sbjct: 241 GAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDN 300

Query: 301 VAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKF 360
           VAIG+FSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP YVTLGNGKKF
Sbjct: 301 VAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKF 360

Query: 361 TGESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420
           TG+SLY+GKPL DSLIPIVYAA+ASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK
Sbjct: 361 TGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 420

Query: 421 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISS 480
           G+VVK+AGGAGMILANTETYGEEQLADAHLLPAAAVGQK GD IK+YIS++ANPTATIS+
Sbjct: 421 GLVVKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISA 480

Query: 481 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRV 540
           G+TRLGVQPSP+VAAFSSRGPN LTPQILKPDLIAPGVNILAGWT G GPTG DSDKR V
Sbjct: 481 GSTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTSGVGPTGLDSDKRHV 540

Query: 541 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLP 600
            FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYK GEA++DVS+GLP
Sbjct: 541 AFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLP 600

Query: 601 TTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGNKNY 660
           +TPFD+GAGHVNPTAALDPGLVYDAT +DYFAFLCALNYSS QIK+I+KKDFTCSGNKNY
Sbjct: 601 STPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNY 660

Query: 661 RLEDLNYPSFAVPLETPSTRGGGD-AAPTTVKYTRTLTNKGAPSTYKASVTSKSPSVKIV 720
           +LEDLNYPSFAV LETPST+GG +  APTTVKYTRTLTNKGAPSTYK SVTSKSPSVKI+
Sbjct: 661 KLEDLNYPSFAVALETPSTKGGAEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIM 720

Query: 721 VEPESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 774
           VEPESLSF + NEQKS+TVTFVAS MPSGSESFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 VEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Tan0013576 vs. ExPASy TrEMBL
Match: A0A0A0LKN0 (Xylem serine proteinase 1 OS=Cucumis sativus OX=3659 GN=Csa_2G354860 PE=3 SV=1)

HSP 1 Score: 1377.1 bits (3563), Expect = 0.0e+00
Identity = 683/771 (88.59%), Postives = 730/771 (94.68%), Query Frame = 0

Query: 3   LQTCRFSSLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWYDS 62
           ++TCR S  WFLLF IS  SCSF  +Q  NQQLKKKTYIIHMD+TNMPQAFDDHFQWYDS
Sbjct: 1   MKTCRVSQ-WFLLFLISFCSCSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDS 60

Query: 63  SLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEFL 122
           SLKSVSDSAQMLYSYNTV+HGFSTRLTVEEAKL+EKQEGI+AVIPE+KYELHTTRTPEFL
Sbjct: 61  SLKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFL 120

Query: 123 GLGKSVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSSSN 182
           GLGKSVSFFPASEKV EVI+GVLDTGVWPELESF+DAGLGP+P +WKGECEVGKNF+SSN
Sbjct: 121 GLGKSVSFFPASEKVSEVIIGVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSN 180

Query: 183 CNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGFAA 242
           CNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGA+LFGFAA
Sbjct: 181 CNRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAA 240

Query: 243 GTARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDNVA 302
           GTARGMAAEARVATYKVCWLGGCF SDILAA+DK+VEDG N+LS+SLGG+S DY+RDNVA
Sbjct: 241 GTARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVA 300

Query: 303 IGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTG 362
           IG+FSA AQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAYVTLGNGKK TG
Sbjct: 301 IGAFSATAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITG 360

Query: 363 ESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGV 422
           ESLYSGKPLP+SL+PIV AA+ASNS+SGSLCL+ TLNPAKV GKIVVCDRGGNSRVQKGV
Sbjct: 361 ESLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGV 420

Query: 423 VVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISSGT 482
           VVKEAGG GMILANTE YGEEQLADAHL+P AAVGQKAGD IKNYISSD+NPTATIS+GT
Sbjct: 421 VVKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGT 480

Query: 483 TRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRVPF 542
           TRLGVQPSPVVAAFSSRGPN LTPQILKPDLIAPGVNILAGWTGGAGPTG DSDKR V F
Sbjct: 481 TRLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAF 540

Query: 543 NIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLPTT 602
           NIISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGE ++D+SNG P+T
Sbjct: 541 NIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEMIQDISNGSPST 600

Query: 603 PFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGNKNYRL 662
           PFD+GAGHVNPTAALDPGLVYD TTDDY AFLCALNYSSLQIK+I+KKDFTC+GNKNY+L
Sbjct: 601 PFDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKL 660

Query: 663 EDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGAPSTYKASVTSKSPSVKIVVEP 722
           EDLNYPSFAVPLETPSTRGG + APTT+KYTRTLTNKGA STYK SVT+KS SVKIVVEP
Sbjct: 661 EDLNYPSFAVPLETPSTRGGENVAPTTIKYTRTLTNKGASSTYKVSVTAKSSSVKIVVEP 720

Query: 723 ESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 774
           ESLSF E NEQKS+TVTF+ASPMPSGS+SFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 ESLSFTEVNEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of Tan0013576 vs. ExPASy TrEMBL
Match: A0A6J1KAX6 (subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111491728 PE=3 SV=1)

HSP 1 Score: 1370.1 bits (3545), Expect = 0.0e+00
Identity = 694/776 (89.43%), Postives = 731/776 (94.20%), Query Frame = 0

Query: 1   MKLQTCRFSSLWFLLFFIS--VFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQ 60
           MK+QTCR S  WFLLF IS    SCSFA +Q      KKKTYIIHMDRTNMPQAFDDHFQ
Sbjct: 1   MKIQTCRVSQ-WFLLFLISSCSCSCSFAAAQ------KKKTYIIHMDRTNMPQAFDDHFQ 60

Query: 61  WYDSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRT 120
           WYDSSLKSVSDSAQ LY YNTV HGFSTRLTVEEAKLIEKQ+GILAVIPE+KY+LHTTRT
Sbjct: 61  WYDSSLKSVSDSAQTLYYYNTVAHGFSTRLTVEEAKLIEKQQGILAVIPELKYDLHTTRT 120

Query: 121 PEFLGLGKSVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNF 180
           PEFLGL KSVSFFPAS KVGEVIVGVLDTGVWPELESF+D GLGPVP +WKGECEVGKNF
Sbjct: 121 PEFLGLEKSVSFFPASVKVGEVIVGVLDTGVWPELESFDDTGLGPVPVSWKGECEVGKNF 180

Query: 181 SSSNCNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLF 240
           +SS+CNRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHG+HTSTTAAGSAVTGASLF
Sbjct: 181 TSSSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLF 240

Query: 241 GFAAGTARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFR 300
           GFAAGTA+GMAAEARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDY+R
Sbjct: 241 GFAAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYR 300

Query: 301 DNVAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGK 360
           DNVAIG+FSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP YVTLGNGK
Sbjct: 301 DNVAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGK 360

Query: 361 KFTGESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRV 420
           KFTG+SLY+GKPL DSLIPIVYAA+ASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRV
Sbjct: 361 KFTGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRV 420

Query: 421 QKGVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATI 480
           QKG+VVK+AGGAGMILANTETYGEEQLADAHLLPAAAVGQK GD IK+YISSDANPTATI
Sbjct: 421 QKGLVVKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISSDANPTATI 480

Query: 481 SSGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKR 540
           S+GTTRLGVQPSP+VAAFSSRGPN LTPQILKPDLIAPGVNILAGWTGG GPTG DSDKR
Sbjct: 481 SAGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGVGPTGLDSDKR 540

Query: 541 RVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNG 600
            V FNIISGTSMSCPHISGLAALLKAAHPDWSPA IRSALMTTAYSTYK GEA++DVS+G
Sbjct: 541 HVAFNIISGTSMSCPHISGLAALLKAAHPDWSPAVIRSALMTTAYSTYKTGEAIQDVSSG 600

Query: 601 LPTTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGNK 660
           LP+TPFD+GAGHVNPTAALDPGLVYDAT +DYFAFLCALNYSS QIK+I+KKDFTCSGNK
Sbjct: 601 LPSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNK 660

Query: 661 NYRLEDLNYPSFAVPLETPSTRGGGD-AAPTTVKYTRTLTNKGAPSTYKASVTSKSPSVK 720
           NY+LEDLNYPSFAV LETPST+GGG+  APTTVKYTRTLTNKGAPSTYK SVTSKSPSVK
Sbjct: 661 NYKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVK 720

Query: 721 IVVEPESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 774
           I+VEPESLSF + NEQKS+TVTFVAS MPSGSESFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 IMVEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 769

BLAST of Tan0013576 vs. ExPASy TrEMBL
Match: A0A6J1KNR9 (subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111495904 PE=3 SV=1)

HSP 1 Score: 1358.6 bits (3515), Expect = 0.0e+00
Identity = 686/762 (90.03%), Postives = 718/762 (94.23%), Query Frame = 0

Query: 12  WFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSA 71
           WFLLF I     SFA +Q       K+TYIIHMDRT+MP+AFDDHFQWYDSSLKSVSDSA
Sbjct: 6   WFLLFLI-----SFAEAQ-------KETYIIHMDRTDMPRAFDDHFQWYDSSLKSVSDSA 65

Query: 72  QMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEFLGLGKSVSFF 131
           QMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPE+KYELHTTRTPEFLGLGKSVSFF
Sbjct: 66  QMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYELHTTRTPEFLGLGKSVSFF 125

Query: 132 PASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSSSNCNRKLIGAR 191
           PASEKVGEVIVGVLDTGVWPELESFNDAG GPVPP+WKGECEVGKNF+SSNCNRKLIGAR
Sbjct: 126 PASEKVGEVIVGVLDTGVWPELESFNDAGFGPVPPSWKGECEVGKNFNSSNCNRKLIGAR 185

Query: 192 YFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGFAAGTARGMAAE 251
           YFS+GYEAAFG +DESQESKSPRDDDGHGTHTSTTAAGS  TGASLFGFAAGTARGMAAE
Sbjct: 186 YFSRGYEAAFGAVDESQESKSPRDDDGHGTHTSTTAAGSPATGASLFGFAAGTARGMAAE 245

Query: 252 ARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDNVAIGSFSAAAQ 311
           ARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDY+RDNVAIG+FSAAAQ
Sbjct: 246 ARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQ 305

Query: 312 GVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGESLYSGKPL 371
           GVFVSCSAGN GPSSGSLSNVAPWITTVGAGTLDRDFP YVTLGNGKKF GESLYSGKPL
Sbjct: 306 GVFVSCSAGNSGPSSGSLSNVAPWITTVGAGTLDRDFPTYVTLGNGKKFIGESLYSGKPL 365

Query: 372 PDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAG 431
            DSLIPIVYA+ ASNS+SGSLCLTSTLNPAKVAGKIVVCDRGGNSR+QKGVVVK+AGG G
Sbjct: 366 SDSLIPIVYASEASNSSSGSLCLTSTLNPAKVAGKIVVCDRGGNSRIQKGVVVKDAGGVG 425

Query: 432 MILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISSGTTRLGVQPSP 491
           MILANT+TYGEEQLADAHLLP AAVGQKAGD IKNYISS+ANPTATISS TTRLGVQPSP
Sbjct: 426 MILANTDTYGEEQLADAHLLPTAAVGQKAGDAIKNYISSNANPTATISSSTTRLGVQPSP 485

Query: 492 VVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRVPFNIISGTSMS 551
           VVAAFSSRGPNFLTPQILKPDLIAPGVNILAGW GGA P GSDS K RVPFNIISGTSMS
Sbjct: 486 VVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWIGGATPAGSDSSKPRVPFNIISGTSMS 545

Query: 552 CPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLPTTPFDVGAGHV 611
           CPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEA++DVS GLP TPF +GAGHV
Sbjct: 546 CPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDVSKGLPATPFGIGAGHV 605

Query: 612 NPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGNKNYRLEDLNYPSFA 671
           NP AALDPGLVYD TT+DYFAFLCALNY+SL+IK+I KKDFTCSGNK YRLEDLNYPSFA
Sbjct: 606 NPMAALDPGLVYDTTTNDYFAFLCALNYTSLEIKVITKKDFTCSGNK-YRLEDLNYPSFA 665

Query: 672 VPLETPSTRGGGDAAPTTVKYTRTLTNKGAPSTYKASVTSKSPSVKIVVEPESLSFGEAN 731
           VPLETPS RG  +AAPTT+KYTRTLTNKG PSTYKA+VTSKSPSVKIVVEP++LSF EAN
Sbjct: 666 VPLETPSIRGDENAAPTTIKYTRTLTNKGTPSTYKATVTSKSPSVKIVVEPQTLSFAEAN 725

Query: 732 EQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 774
           EQKS+TVTF+ASPMPSG+ESFARLEWSDGKHIVGSPIAFTWT
Sbjct: 726 EQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 754

BLAST of Tan0013576 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 992.3 bits (2564), Expect = 2.2e-289
Identity = 489/737 (66.35%), Postives = 591/737 (80.19%), Query Frame = 0

Query: 39  TYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEAKLIEK 98
           TYI+HM ++ MP +FD H  WYDSSL+S+SDSA++LY+Y   +HGFSTRLT EEA  +  
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYENAIHGFSTRLTQEEADSLMT 90

Query: 99  QEGILAVIPEVKYELHTTRTPEFLGLGK-SVSFFPASEKVGEVIVGVLDTGVWPELESFN 158
           Q G+++V+PE +YELHTTRTP FLGL + +   FP +    +V+VGVLDTGVWPE +S++
Sbjct: 91  QPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYS 150

Query: 159 DAGLGPVPPTWKGECEVGKNFSSSNCNRKLIGARYFSKGYEAAFGTIDESQESKSPRDDD 218
           D G GP+P +WKG CE G NF++S CNRKLIGAR+F++GYE+  G IDES+ES+SPRDDD
Sbjct: 151 DEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDD 210

Query: 219 GHGTHTSTTAAGSAVTGASLFGFAAGTARGMAAEARVATYKVCWLGGCFGSDILAAIDKA 278
           GHGTHTS+TAAGS V GASL G+A+GTARGMA  ARVA YKVCWLGGCF SDILAAIDKA
Sbjct: 211 GHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKA 270

Query: 279 VEDGVNVLSLSLGGSSPDYFRDNVAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWIT 338
           + D VNVLS+SLGG   DY+RD VAIG+F+A  +G+ VSCSAGN GPSS SLSNVAPWIT
Sbjct: 271 IADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWIT 330

Query: 339 TVGAGTLDRDFPAYVTLGNGKKFTGESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTST 398
           TVGAGTLDRDFPA   LGNGK FTG SL+ G+ LPD L+P +YA NASN+T+G+LC+T T
Sbjct: 331 TVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGT 390

Query: 399 LNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVG 458
           L P KV GKIV+CDRG N+RVQKG VVK AGG GMILANT   GEE +ADAHLLPA  VG
Sbjct: 391 LIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVG 450

Query: 459 QKAGDVIKNYISSDANPTATISSGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPG 518
           +KAGD+I++Y+++D NPTA+IS   T +GV+PSPVVAAFSSRGPN +TP ILKPDLIAPG
Sbjct: 451 EKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPG 510

Query: 519 VNILAGWTGGAGPTGSDSDKRRVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSA 578
           VNILA WTG AGPTG  SD RRV FNIISGTSMSCPH+SGLAALLK+ HP+WSPAAIRSA
Sbjct: 511 VNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSA 570

Query: 579 LMTTAYSTYKNGEALKDVSNGLPTTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCAL 638
           LMTTAY TYK+G+ L D++ G P+TPFD GAGHV+PT A +PGL+YD TT+DY  FLCAL
Sbjct: 571 LMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCAL 630

Query: 639 NYSSLQIKIIAKKDFTCSGNKNYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLT 698
           NY+S QI+ ++++++TC  +K+Y + DLNYPSFAV ++     G G       KYTRT+T
Sbjct: 631 NYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVD-----GVG-----AYKYTRTVT 690

Query: 699 NKGAPSTYKASVTSKSPSVKIVVEPESLSFGEANEQKSFTVTF-VASPMPSGSESFARLE 758
           + G   TY   VTS++  VKI VEP  L+F EANE+KS+TVTF V S  PSGS SF  +E
Sbjct: 691 SVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIE 750

Query: 759 WSDGKHIVGSPIAFTWT 774
           WSDGKH+VGSP+A +WT
Sbjct: 751 WSDGKHVVGSPVAISWT 757

BLAST of Tan0013576 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 766.5 bits (1978), Expect = 2.0e-221
Identity = 410/740 (55.41%), Postives = 505/740 (68.24%), Query Frame = 0

Query: 37  KKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLYSYNTVVHGFSTRLTVEEA-KL 96
           KKTYII ++ ++ P++F  H  WY S L S S    +LY+Y T  HGFS  L   EA  L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 97  IEKQEGILAVIPEVKYELHTTRTPEFLGLGKSVSFFPASEKVGEVIVGVLDTGVWPELES 156
           +     IL +  +  Y LHTTRTPEFLGL               VI+GVLDTGVWPE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 157 FNDAGLGPVPPTWKGECEVGKNFSSSNCNRKLIGARYFSKGYE-AAFGTIDESQESKSPR 216
           F+D  +  +P  WKGECE G +F S  CN+KLIGAR FSKG++ A+ G     +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 217 DDDGHGTHTSTTAAGSAVTGASLFGFAAGTARGMAAEARVATYKVCWLGGCFGSDILAAI 276
           D DGHGTHTSTTAAGSAV  AS  G+AAGTARGMA  ARVATYKVCW  GCFGSDILAA+
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 277 DKAVEDGVNVLSLSLGGSSPDYFRDNVAIGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAP 336
           D+A+ DGV+VLSLSLGG S  Y+RD +AIG+FSA  +GVFVSCSAGN GP+  S++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 337 WITTVGAGTLDRDFPAYVTLGNGKKFTGESLYSGKPLPDSLIPIVYAANASNSTSGSLCL 396
           W+ TVGAGTLDRDFPA+  LGNGK+ TG SLYSG  +    + +VY  N  NS+S +LCL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 397 TSTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMILANTETYGEEQLADAHLLPAA 456
             +L+ + V GKIVVCDRG N+RV+KG VV++AGG GMI+ANT   GEE +AD+HLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 457 AVGQKAGDVIKNYISSDANPTATISSGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLI 516
           AVG+K GD+++ Y+ SD+ PTA +    T L V+PSPVVAAFSSRGPN +TP+ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 517 APGVNILAGWTGGAGPTGSDSDKRRVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAI 576
            PGVNILAGW+   GPTG D D RR  FNI+SGTSMSCPHISGLA LLKAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 577 RSALMTTAYSTYKNGEALKDVSNGLPTTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFL 636
           +SALMTTAY        L D ++   + P+  G+GHV+P  AL PGLVYD +T++Y  FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 637 CALNYSSLQIKIIAKKDFTCSGNKNYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTR 696
           C+L+Y+   I  I K+       K      LNYPSF+V         GG      V+YTR
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF-------GG---KRVVRYTR 686

Query: 697 TLTNKGAPSTYKASVTSKSPSVKIVVEPESLSFGEANEQKSFTVTFVASPMPS--GSESF 756
            +TN GA S+      + +PSV I V+P  LSF    E+K +TVTFV+    S      F
Sbjct: 687 EVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEF 746

Query: 757 ARLEWSDGKHIVGSPIAFTW 773
             + WS+ +H V SP+AF+W
Sbjct: 747 GSITWSNPQHEVRSPVAFSW 751

BLAST of Tan0013576 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 759.6 bits (1960), Expect = 2.4e-219
Identity = 406/774 (52.45%), Postives = 518/774 (66.93%), Query Frame = 0

Query: 15  LFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVS------ 74
           LF I   +  F  ++   Q   KKTY+IHMD++ MP  + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 75  ---DSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEFLGLG 134
              ++ ++LY+Y T  HG + +LT EEA+ +E+++G++AVIPE +YELHTTR+P FLGL 
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 135 KSVSFFPASEKV--GEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSSSNC 194
           +  S    +E+V   +V+VGVLDTG+WPE ESFND G+ PVP TW+G CE GK F   NC
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 195 NRKLIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGFAAG 254
           NRK++GAR F +GYEAA G IDE  E KSPRD DGHGTHT+ T AGS V GA+LFGFA G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 255 TARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDNVAI 314
           TARGMA +ARVA YKVCW+GGCF SDIL+A+D+AV DGV VLS+SLGG    Y RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 315 GSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGE 374
            +F A   GVFVSCSAGNGGP   SL+NV+PWITTVGA T+DRDFPA V +G  + F G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 375 SLYSGKPL--PDSLIPIVY-AANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 434
           SLY G+ +   +   P+VY   NAS+    S CL   L+   VAGKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 435 GVVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISS 494
           G VVK AGG GM+L NT T GEE +AD+H+LPA AVG+K G +IK Y  +    TA++  
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 495 GTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRV 554
             TR+G++PSPVVAAFSSRGPNFL+ +ILKPDL+APGVNILA WTG   P+   SD RRV
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 555 PFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLP 614
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTTAY      + L D S   P
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 615 TTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAK-KDFTCSGNKN 674
           ++P+D GAGH++P  A DPGLVYD    +YF FLC  + S  Q+K+  K  + TC     
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 675 YRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGAP-STYKASVTSKSPSVKI 734
               +LNYP+ +            +     +   RT+TN G   S+YK SV S      +
Sbjct: 672 KNPGNLNYPAISALFPE-------NTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKGASV 731

Query: 735 VVEPESLSFGEANEQKSFTVTFVASPMPSGSESFARLEWSDGKHIVGSPIAFTW 773
            V+P++L+F   +++ S+TVTF  +        F  L W    H V SP+  TW
Sbjct: 732 TVQPKTLNFTSKHQKLSYTVTF-RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Tan0013576 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 730.3 bits (1884), Expect = 1.6e-210
Identity = 398/780 (51.03%), Postives = 516/780 (66.15%), Query Frame = 0

Query: 10  SLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSD 69
           SL  + F   +  C F+ S + +  L  ++YI+H+ R++ P  F  H  W+ S L+S+  
Sbjct: 5   SLSSIFFVFPLLLCFFSPSSSSSDGL--ESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPS 64

Query: 70  SAQ---MLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEFLGLGK 129
           S Q   +LYSY+  VHGFS RL+  +   + +   +++VIP+   E+HTT TP FLG  +
Sbjct: 65  SPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQ 124

Query: 130 SVSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSSSNCNRK 189
           +   +  S    +VIVGVLDTG+WPE  SF+D+GLGP+P TWKGECE+G +F +S+CNRK
Sbjct: 125 NSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRK 184

Query: 190 LIGARYFSKGY-EAAFGTIDE-SQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGFAAGT 249
           LIGAR F +GY     GT    ++ES+SPRD +GHGTHT++TAAGS V  ASL+ +A GT
Sbjct: 185 LIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGT 244

Query: 250 ARGMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLG--GSSPDYFRDNVA 309
           A GMA++AR+A YK+CW GGC+ SDILAA+D+AV DGV+V+SLS+G  GS+P+Y  D++A
Sbjct: 245 ATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIA 304

Query: 310 IGSFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTG 369
           IG+F A   G+ VSCSAGN GP+  + +N+APWI TVGA T+DR+F A    G+GK FTG
Sbjct: 305 IGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTG 364

Query: 370 ESLYSGKPLPDSLIPIVYAANASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGV 429
            SLY+G+ LPDS + +VY    S      LC    LN + V GKIV+CDRGGN+RV+KG 
Sbjct: 365 TSLYAGESLPDSQLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGS 424

Query: 430 VVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYISSDANPTATISSGT 489
            VK AGGAGMILANT   GEE  AD+HL+PA  VG KAGD I++YI +  +PTA IS   
Sbjct: 425 AVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLG 484

Query: 490 TRLGVQ-PSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDKRRVP 549
           T +G   PSP VAAFSSRGPN LTP ILKPD+IAPGVNILAGWTG  GPT  D D RRV 
Sbjct: 485 TLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQ 544

Query: 550 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSNGLPT 609
           FNIISGTSMSCPH+SGLAALL+ AHPDWSPAAI+SAL+TTAY    +GE ++D++ G  +
Sbjct: 545 FNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSS 604

Query: 610 TPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDF---TCSGNK 669
             F  GAGHV+P  AL+PGLVYD    +Y AFLCA+ Y    I +  +       C  +K
Sbjct: 605 NSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSK 664

Query: 670 NYRLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKGAPSTYKASVTSKSP-SVK 729
                DLNYPSF+V   +             VKY R + N G+       V  KSP +V+
Sbjct: 665 LRTAGDLNYPSFSVVFASTG---------EVVKYKRVVKNVGSNVDAVYEVGVKSPANVE 724

Query: 730 IVVEPESLSFGEANEQKSFTVTFVASPMPSG-----SESFARLEWSDGKHIVGSPIAFTW 773
           I V P  L+F +      + VTF +  +  G        F  +EW+DG+H+V SP+A  W
Sbjct: 725 IDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Tan0013576 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 701.0 bits (1808), Expect = 1.0e-201
Identity = 387/781 (49.55%), Postives = 510/781 (65.30%), Query Frame = 0

Query: 10  SLWFLLFFISVFSCSFAVSQNKNQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSD 69
           + +F  FF+   S   + + + N      TYI+H+D    P  F  HF WY SSL S++ 
Sbjct: 2   AFFFYFFFLLTLSSPSSSASSSN----SLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTS 61

Query: 70  S-AQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEFLGLGKS- 129
           S   ++++Y+TV HGFS RLT ++A  +     +++VIPE    LHTTR+PEFLGL  + 
Sbjct: 62  SPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTD 121

Query: 130 -VSFFPASEKVGEVIVGVLDTGVWPELESFNDAGLGPVPPTWKGECEVGKNFSSSNCNRK 189
                  S+   ++++GV+DTGVWPE  SF+D GLGPVP  WKG+C   ++F  S CNRK
Sbjct: 122 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 181

Query: 190 LIGARYFSKGYEAAFGTIDESQESKSPRDDDGHGTHTSTTAAGSAVTGASLFGFAAGTAR 249
           L+GAR+F  GYEA  G ++E+ E +SPRD DGHGTHT++ +AG  V  AS  G+A G A 
Sbjct: 182 LVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAA 241

Query: 250 GMAAEARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYFRDNVAIGSF 309
           GMA +AR+A YKVCW  GC+ SDILAA D AV DGV+V+SLS+GG    Y+ D +AIG+F
Sbjct: 242 GMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAF 301

Query: 310 SAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKFTGESLY 369
            A  +G+FVS SAGNGGP + +++NVAPW+TTVGAGT+DRDFPA V LGNGK  +G S+Y
Sbjct: 302 GAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVY 361

Query: 370 SGKPL-PDSLIPIVYAAN--ASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGVV 429
            G  L P  + P+VY  +    +  S SLCL  +L+P  V GKIV+CDRG NSR  KG +
Sbjct: 362 GGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEI 421

Query: 430 VKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKAGDVIKNYIS------SDANPTAT 489
           V++ GG GMI+AN    GE  +AD H+LPA +VG   GD I+ YIS      S  +PTAT
Sbjct: 422 VRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTAT 481

Query: 490 ISSGTTRLGVQPSPVVAAFSSRGPNFLTPQILKPDLIAPGVNILAGWTGGAGPTGSDSDK 549
           I    TRLG++P+PVVA+FS+RGPN  TP+ILKPD+IAPG+NILA W    GP+G  SD 
Sbjct: 482 IVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 541

Query: 550 RRVPFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEALKDVSN 609
           RR  FNI+SGTSM+CPH+SGLAALLKAAHPDWSPAAIRSAL+TTAY+   +GE + D S 
Sbjct: 542 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDEST 601

Query: 610 GLPTTPFDVGAGHVNPTAALDPGLVYDATTDDYFAFLCALNYSSLQIKIIAKKDFTCSGN 669
           G  ++  D G+GHV+PT A+DPGLVYD T+ DY  FLC  NY+   I  I ++   C G 
Sbjct: 602 GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGA 661

Query: 670 KNY-RLEDLNYPSFAVPLETPSTRGGGDAAPTTVKYTRTLTNKG-APSTYKASVTSKSPS 729
           +    + +LNYPSF+V  +       G++  +T  + RT+TN G + S Y+  +      
Sbjct: 662 RRAGHVGNLNYPSFSVVFQQ-----YGESKMST-HFIRTVTNVGDSDSVYEIKIRPPR-G 721

Query: 730 VKIVVEPESLSFGEANEQKSF-----TVTFVASPMPSGSESFARLEWSDGKHIVGSPIAF 772
             + VEPE LSF    ++ SF     T     SP  +  E+   + WSDGK  V SP+  
Sbjct: 722 TTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVET-GHIVWSDGKRNVTSPLVV 770