Tan0013517 (gene) Snake gourd v1

Overview


Name	Tan0013517
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	(R)-specific enoyl-CoA hydratase
Location	LG05: 2100169 .. 2100865 (+)
RNA-Seq Expression	Tan0013517
Synteny	Tan0013517

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTAAGTGGTAGATGTTTATTCTCAACTTACATTTCTTCTTCTTTGAAATGCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTGGGAGATATTTTGAGCCATACTCGAATATTCACCAGTGAAGATGTGTTGGAATATTCCAAGGTGAGTCATGACTCAAATCCTCTGCATTTTGATTCTGAATTAGCCCGACGTGCTGGATTCGATGATCGTCTTGTTCACGGGATGCTTGTTGCTTCAATTTTCCCTCATATCATTTCCTCTCATTTTGTAAGTCTAGCAAGTCAGTCTTGTTTATGCTTGTTTAAGCTGTTTTAATGTATGGTCTATACATTGAAGATGGTCTGATTTATGGTATATGTTGAGGATGATTGTAGCCAGGAGCTATTTACGTGTCGCAAAGCTTGAATTTTAAGTTACCAGTCTATGTTGGAGAGAAGATCATTGGCCAGGTAGAAGCAATCGATTTTAGAGAAAATAAGAAGAGATATCTGTAAGTGCTTGTTCTTGGCAAATGGAATCTCTAACTTCTCAGGTTCAAATTTCAAGCTATCATTTCATTGAGTTGAGTACTTGTTGCTTCTGATTTAGGGTGAAATTCAAAACGAAGTGCTTGAGGAATGGTGATGAACTCGTTCTTGAAGGCGAGGCAAGGGCCATACTACCTTCTGATTTTCATTCGTCAGACAGATAG

mRNA sequence

ATGCTAAGTGGTAGATGTTTATTCTCAACTTACATTTCTTCTTCTTTGAAATGCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTGGGAGATATTTTGAGCCATACTCGAATATTCACCAGTGAAGATGTGTTGGAATATTCCAAGGTGAGTCATGACTCAAATCCTCTGCATTTTGATTCTGAATTAGCCCGACGTGCTGGATTCGATGATCGTCTTGTTCACGGGATGCTTGTTGCTTCAATTTTCCCTCATATCATTTCCTCTCATTTTCCAGGAGCTATTTACGTGTCGCAAAGCTTGAATTTTAAGTTACCAGTCTATGTTGGAGAGAAGATCATTGGCCAGGTAGAAGCAATCGATTTTAGAGAAAATAAGAAGAGATATCTGGTGAAATTCAAAACGAAGTGCTTGAGGAATGGTGATGAACTCGTTCTTGAAGGCGAGGCAAGGGCCATACTACCTTCTGATTTTCATTCGTCAGACAGATAG

Coding sequence (CDS)

ATGCTAAGTGGTAGATGTTTATTCTCAACTTACATTTCTTCTTCTTTGAAATGCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTGGGAGATATTTTGAGCCATACTCGAATATTCACCAGTGAAGATGTGTTGGAATATTCCAAGGTGAGTCATGACTCAAATCCTCTGCATTTTGATTCTGAATTAGCCCGACGTGCTGGATTCGATGATCGTCTTGTTCACGGGATGCTTGTTGCTTCAATTTTCCCTCATATCATTTCCTCTCATTTTCCAGGAGCTATTTACGTGTCGCAAAGCTTGAATTTTAAGTTACCAGTCTATGTTGGAGAGAAGATCATTGGCCAGGTAGAAGCAATCGATTTTAGAGAAAATAAGAAGAGATATCTGGTGAAATTCAAAACGAAGTGCTTGAGGAATGGTGATGAACTCGTTCTTGAAGGCGAGGCAAGGGCCATACTACCTTCTGATTTTCATTCGTCAGACAGATAG

Protein sequence

MLSGRCLFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSELARRAGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAIDFRENKKRYLVKFKTKCLRNGDELVLEGEARAILPSDFHSSDR

Homology

[Close]

BLAST of Tan0013517 vs. ExPASy Swiss-Prot
Match: O32472 ((R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 6.5e-18
Identity = 49/131 (37.40%), Postives = 75/131 (57.25%), Query Frame = 0


Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSELARRAGFDDRLVHGMLVASIFPHI 86
           L+VG     ++ F + +V  ++ +S D NPLH D   A    F+  +VHGML+AS+F  +
Sbjct: 6   LEVGQKARLSKRFGAAEVAAFAALSEDFNPLHLDPAFAATTAFERPIVHGMLLASLFSGL 65

Query: 87  ISSHFP--GAIYVSQSLNFKLPVYVGEKIIGQVEAIDFRENKKRYLVKFKTKCLRNGDEL 146
           +    P  G+IY+ QSL+FKLPV+VG+++  +VE    RE+K   +    T+    G  L
Sbjct: 66  LGQQLPGKGSIYLGQSLSFKLPVFVGDEVTAEVEVTALREDKP--IATLTTRIFTQGGAL 125

Query: 147 VLEGEARAILP 156
            + GEA   LP
Sbjct: 126 AVTGEAVVKLP 134

[Close]

BLAST of Tan0013517 vs. ExPASy Swiss-Prot
Match: Q2RQ36 ((R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=phaJ PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.1e-14
Identity = 45/132 (34.09%), Postives = 71/132 (53.79%), Query Frame = 0


Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSELARRAGFDDRLVHGMLVASIFPHI 86
           +K G   S  +  +  D+  ++ +S D+NP H + + A+   F  R+ HGML A     +
Sbjct: 14  IKEGQSASLAKTISESDIYLFAGLSMDTNPAHVNEDYAQTTVFKTRIAHGMLSAGFISAV 73

Query: 87  ISSHF--PGAIYVSQSLNFKLPVYVGEKIIGQVEAIDFRENKKRYLVKFKTKCLRNGDEL 146
           + +    PGAIYV+QSL FK PV +G+ +   V        KK   V F+T C   G ++
Sbjct: 74  LGTRLPGPGAIYVNQSLKFKAPVRIGDTVTATVTVTGLVPEKK--FVTFRTTCTVAG-KV 133

Query: 147 VLEGEARAILPS 157
           V+EGEA  ++P+
Sbjct: 134 VIEGEATVMVPA 142

[Close]

BLAST of Tan0013517 vs. ExPASy Swiss-Prot
Match: C5AZ74 (3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) OX=272630 GN=croR PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.7e-14
Identity = 39/118 (33.05%), Postives = 68/118 (57.63%), Query Frame = 0


Query: 40  TSEDVLEYSKVSHDSNPLHFDSELARRAGFDDRLVHGMLVASIFPHIISSHF--PGAIYV 99
           +S DV+ +++++ D NP+H     A R  F  R+ HG+  A +   ++ +    PGA+Y+
Sbjct: 26  SSSDVVGFAEITGDRNPIHLSEHFAARTPFGTRIAHGLYTAGLISAVLGTRLPGPGAVYI 85

Query: 100 SQSLNFKLPVYVGEKIIGQVEAIDFRENKKRYLVKFKTKCLRNGDELVLEGEARAILP 156
           SQ+LNF+ PV +G+ +  +VE  +    ++R   +    C   GDE+VL+GEA   +P
Sbjct: 86  SQTLNFRAPVRIGDIVEVKVEVAELIPERRR--ARLACTC-SVGDEVVLDGEALVKVP 140

[Close]

BLAST of Tan0013517 vs. ExPASy Swiss-Prot
Match: P86397 (Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 GN=HTD2 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.7e-14
Identity = 38/132 (28.79%), Postives = 72/132 (54.55%), Query Frame = 0


Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSELARRAGFDDRLVHGMLVASIFPHI 86
           +KVGD     R FT  DV  +S+++ D NPLH + + A+   F + +VHG+L+  +   +
Sbjct: 36  IKVGDRAELRRAFTQTDVATFSELTGDVNPLHLNEDFAKHTKFGNTIVHGVLINGLISAL 95

Query: 87  ISSHF--PGAIYVSQSLNFKLPVYVGEKIIGQVEAIDFRENKKRYLVKFKTKC-LRNGDE 146
           + +    PG +++SQ ++F  P+Y+GE ++   E     +  KR++      C +    +
Sbjct: 96  LGTKMPGPGCVFLSQEISFPAPLYIGEVVLASAEV----KKLKRFIAIIAVSCSVIESKK 155

Query: 147 LVLEGEARAILP 156
            V+EG  + ++P
Sbjct: 156 TVMEGWVKVMVP 163

[Close]

BLAST of Tan0013517 vs. ExPASy Swiss-Prot
Match: Q9VXJ0 (Peroxisomal multifunctional enzyme type 2 OS=Drosophila melanogaster OX=7227 GN=Mfe2 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.8e-04
Identity = 31/109 (28.44%), Postives = 51/109 (46.79%), Query Frame = 0


Query: 40  TSEDVLEYSKVSHDSNPLHFDSELARRAGFDDRLVHGMLVASIFPHIISSHFPG---AIY 99
           TSED     ++S D NPLH D ++A  AGF   ++HG+         + + F     A++
Sbjct: 483 TSEDQAALYRLSGDKNPLHIDPQMALLAGFKTPILHGLCTLGFSVRAVLAQFADNNPALF 542

Query: 100 VSQSLNFKLPVYVGEKIIGQVEAIDFRENKKRYLVKFKTKCLRNGDELV 146
            +  + F  PV     I GQ   +D  +   R  + F+T  +  G E++
Sbjct: 543 KAVKVRFSGPV-----IPGQTLRVDLWKQGTR--INFRTVVVETGKEVI 584

[Close]

BLAST of Tan0013517 vs. NCBI nr
Match: XP_004149553.1 (uncharacterized protein LOC101214584 [Cucumis sativus] >KGN47303.1 hypothetical protein Csa_022963 [Cucumis sativus])

HSP 1 Score: 278.5 bits (711), Expect = 3.7e-71
Identity = 138/156 (88.46%), Postives = 150/156 (96.15%), Query Frame = 0


Query: 7   LFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSELARR 66
           LFSTYISSSLKCFSSSTS+VLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFDS+LA+R
Sbjct: 6   LFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDSKLAQR 65

Query: 67  AGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAIDFRENK 126
           AGF+  LVHG+LVAS+FPHIISSH+PGAIYVSQSLNFKLPVYVGEKI+GQVEAI+ RENK
Sbjct: 66  AGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENK 125

Query: 127 KRYLVKFKTKCLRNGDELVLEGEARAILPSDFHSSD 163
           KRYL KFKTKCLRNGDELVLEGEARAILP DFHS++
Sbjct: 126 KRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTN 161

[Close]

BLAST of Tan0013517 vs. NCBI nr
Match: XP_022985629.1 (uncharacterized protein LOC111483634 [Cucurbita maxima])

HSP 1 Score: 278.1 bits (710), Expect = 4.9e-71
Identity = 143/162 (88.27%), Postives = 150/162 (92.59%), Query Frame = 0


Query: 1   MLSGRCLFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS   L S+YISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SELARRAGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAI 120
           S LARRAGFDDRLVHGMLVAS+FP +ISSHFPGAIYVSQSLNFKLPVYVGE II +VEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DFRENKKRYLVKFKTKCLRNGDELVLEGEARAILPSDFHSSD 163
           D RE KKRYL KF+TKCLRNGDELVL+GEARAILPSDF S+D
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTD 162

[Close]

BLAST of Tan0013517 vs. NCBI nr
Match: XP_038901209.1 ((R)-specific enoyl-CoA hydratase [Benincasa hispida])

HSP 1 Score: 277.7 bits (709), Expect = 6.4e-71
Identity = 141/162 (87.04%), Postives = 153/162 (94.44%), Query Frame = 0


Query: 1   MLSGRCLFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS R LFS +IS+SLKCFSSSTSNVLKVGDILS+TRIFTS+DVLEYSKVSHDSNPLHFD
Sbjct: 1   MLS-RGLFSMHISTSLKCFSSSTSNVLKVGDILSYTRIFTSKDVLEYSKVSHDSNPLHFD 60

Query: 61  SELARRAGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAI 120
           SELARRAGF+ RLVHGMLVAS+FPHIISSHFPGAIYVSQSLNFKLPVYVGE I+GQVEAI
Sbjct: 61  SELARRAGFNGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGENIVGQVEAI 120

Query: 121 DFRENKKRYLVKFKTKCLRNGDELVLEGEARAILPSDFHSSD 163
           D RENKKRYL KF TKC+RNG E+VLEGEARAI+PSDFHS++
Sbjct: 121 DLRENKKRYLAKFITKCMRNGHEVVLEGEARAIIPSDFHSTN 161

[Close]

BLAST of Tan0013517 vs. NCBI nr
Match: XP_008463911.1 (PREDICTED: (R)-specific enoyl-CoA hydratase [Cucumis melo])

HSP 1 Score: 277.7 bits (709), Expect = 6.4e-71
Identity = 142/162 (87.65%), Postives = 153/162 (94.44%), Query Frame = 0


Query: 1   MLSGRCLFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS R LFST+ISSSLKCFSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 45  MLS-RGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 104

Query: 61  SELARRAGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAI 120
            ELARRAGF+  LVHG+LVAS+FPHIISSH+PGAIYVSQSLNFKLPVYVGEKI+GQVEAI
Sbjct: 105 PELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 164

Query: 121 DFRENKKRYLVKFKTKCLRNGDELVLEGEARAILPSDFHSSD 163
           + RENKKRYL KFKTKCLRNGDELVLEGEARAILP+ FHS++
Sbjct: 165 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 205

[Close]

BLAST of Tan0013517 vs. NCBI nr
Match: KAA0035311.1 ((R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa] >TYK14325.1 (R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa])

HSP 1 Score: 277.7 bits (709), Expect = 6.4e-71
Identity = 142/162 (87.65%), Postives = 153/162 (94.44%), Query Frame = 0


Query: 1   MLSGRCLFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS R LFST+ISSSLKCFSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLS-RGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SELARRAGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAI 120
            ELARRAGF+  LVHG+LVAS+FPHIISSH+PGAIYVSQSLNFKLPVYVGEKI+GQVEAI
Sbjct: 61  PELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120

Query: 121 DFRENKKRYLVKFKTKCLRNGDELVLEGEARAILPSDFHSSD 163
           + RENKKRYL KFKTKCLRNGDELVLEGEARAILP+ FHS++
Sbjct: 121 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 161

[Close]

BLAST of Tan0013517 vs. ExPASy TrEMBL
Match: A0A0A0KCN3 (MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.8e-71
Identity = 138/156 (88.46%), Postives = 150/156 (96.15%), Query Frame = 0


Query: 7   LFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSELARR 66
           LFSTYISSSLKCFSSSTS+VLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFDS+LA+R
Sbjct: 6   LFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDSKLAQR 65

Query: 67  AGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAIDFRENK 126
           AGF+  LVHG+LVAS+FPHIISSH+PGAIYVSQSLNFKLPVYVGEKI+GQVEAI+ RENK
Sbjct: 66  AGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENK 125

Query: 127 KRYLVKFKTKCLRNGDELVLEGEARAILPSDFHSSD 163
           KRYL KFKTKCLRNGDELVLEGEARAILP DFHS++
Sbjct: 126 KRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTN 161

[Close]

BLAST of Tan0013517 vs. ExPASy TrEMBL
Match: A0A6J1JDU3 (uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.4e-71
Identity = 143/162 (88.27%), Postives = 150/162 (92.59%), Query Frame = 0


Query: 1   MLSGRCLFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS   L S+YISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SELARRAGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAI 120
           S LARRAGFDDRLVHGMLVAS+FP +ISSHFPGAIYVSQSLNFKLPVYVGE II +VEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DFRENKKRYLVKFKTKCLRNGDELVLEGEARAILPSDFHSSD 163
           D RE KKRYL KF+TKCLRNGDELVL+GEARAILPSDF S+D
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTD 162

[Close]

BLAST of Tan0013517 vs. ExPASy TrEMBL
Match: A0A5A7T147 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00540 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.1e-71
Identity = 142/162 (87.65%), Postives = 153/162 (94.44%), Query Frame = 0


Query: 1   MLSGRCLFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS R LFST+ISSSLKCFSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLS-RGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SELARRAGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAI 120
            ELARRAGF+  LVHG+LVAS+FPHIISSH+PGAIYVSQSLNFKLPVYVGEKI+GQVEAI
Sbjct: 61  PELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120

Query: 121 DFRENKKRYLVKFKTKCLRNGDELVLEGEARAILPSDFHSSD 163
           + RENKKRYL KFKTKCLRNGDELVLEGEARAILP+ FHS++
Sbjct: 121 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 161

[Close]

BLAST of Tan0013517 vs. ExPASy TrEMBL
Match: A0A1S3CLU3 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.1e-71
Identity = 142/162 (87.65%), Postives = 153/162 (94.44%), Query Frame = 0


Query: 1   MLSGRCLFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS R LFST+ISSSLKCFSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 45  MLS-RGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 104

Query: 61  SELARRAGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAI 120
            ELARRAGF+  LVHG+LVAS+FPHIISSH+PGAIYVSQSLNFKLPVYVGEKI+GQVEAI
Sbjct: 105 PELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 164

Query: 121 DFRENKKRYLVKFKTKCLRNGDELVLEGEARAILPSDFHSSD 163
           + RENKKRYL KFKTKCLRNGDELVLEGEARAILP+ FHS++
Sbjct: 165 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 205

[Close]

BLAST of Tan0013517 vs. ExPASy TrEMBL
Match: A0A6J1FRZ2 (uncharacterized protein LOC111448279 OS=Cucurbita moschata OX=3662 GN=LOC111448279 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.7e-69
Identity = 140/156 (89.74%), Postives = 146/156 (93.59%), Query Frame = 0


Query: 1   MLSGRCLFSTYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS   L S+ ISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSCISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SELARRAGFDDRLVHGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAI 120
           S LARRAGFDDRLVHGMLVAS+FPH+ISSHFPGAIYVSQSLNFKLPVYVGE II +VEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DFRENKKRYLVKFKTKCLRNGDELVLEGEARAILPS 157
           D RE KKRYLVKF+TKCLRNGDELVL+GEARAILPS
Sbjct: 121 DLREIKKRYLVKFQTKCLRNGDELVLDGEARAILPS 156

[Close]

BLAST of Tan0013517 vs. TAIR 10
Match: AT5G60335.1 (Thioesterase superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 1.8e-47
Identity = 89/143 (62.24%), Postives = 120/143 (83.92%), Query Frame = 0


Query: 16  LKCFSS-STSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSELARRAGFDDRLV 75
           L+ FSS ++  +LKVGD+L  TR+F+SED+  Y++VSHD NPLHFD E AR+AGF++RLV
Sbjct: 14  LRSFSSVASKTLLKVGDVLRETRVFSSEDIKAYAEVSHDWNPLHFDPESARKAGFENRLV 73

Query: 76  HGMLVASIFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIIGQVEAIDFRENKKRYLVKFK 135
           HGMLV+S+FP IIS+HFPGA+YVSQSL+F+ PVY+G++I+G V+AI  RE K +Y+VKF 
Sbjct: 74  HGMLVSSMFPRIISAHFPGAVYVSQSLHFRSPVYIGDEILGLVQAIALRETKNKYIVKFS 133

Query: 136 TKCLRNGDEL-VLEGEARAILPS 157
           TKC +N +EL V++GEA AILP+
Sbjct: 134 TKCFKNHNELVVIDGEATAILPN 156

The following BLAST results are available for this feature:

BLAST of Tan0013517 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O32472	6.5e-18	37.40	(R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1	[more]
Q2RQ36	1.1e-14	34.09	(R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / A...	[more]
C5AZ74	5.7e-14	33.05	3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 ...	[more]
P86397	5.7e-14	28.79	Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 ...	[more]
Q9VXJ0	1.8e-04	28.44	Peroxisomal multifunctional enzyme type 2 OS=Drosophila melanogaster OX=7227 GN=...	[more]

BLAST of Tan0013517 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149553.1	3.7e-71	88.46	uncharacterized protein LOC101214584 [Cucumis sativus] >KGN47303.1 hypothetical ...	[more]
XP_022985629.1	4.9e-71	88.27	uncharacterized protein LOC111483634 [Cucurbita maxima]	[more]
XP_038901209.1	6.4e-71	87.04	(R)-specific enoyl-CoA hydratase [Benincasa hispida]	[more]
XP_008463911.1	6.4e-71	87.65	PREDICTED: (R)-specific enoyl-CoA hydratase [Cucumis melo]	[more]
KAA0035311.1	6.4e-71	87.65	(R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa] >TYK14325.1 (R)-spec...	[more]

BLAST of Tan0013517 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCN3	1.8e-71	88.46	MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 P...	[more]
A0A6J1JDU3	2.4e-71	88.27	uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634...	[more]
A0A5A7T147	3.1e-71	87.65	(R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CLU3	3.1e-71	87.65	(R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV...	[more]
A0A6J1FRZ2	1.7e-69	89.74	uncharacterized protein LOC111448279 OS=Cucurbita moschata OX=3662 GN=LOC1114482...	[more]

BLAST of Tan0013517 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60335.1	1.8e-47	62.24	Thioesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.129.10	Hotdog Thioesterase	coord: 16..158 e-value: 1.1E-36 score: 127.4
None	No IPR available	PANTHER	PTHR43437:SF3	HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED	coord: 9..156
None	No IPR available	PANTHER	PTHR43437	HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED	coord: 9..156
None	No IPR available	CDD	cd03449	R_hydratase	coord: 27..154 e-value: 1.28989E-46 score: 146.536
IPR002539	MaoC-like dehydratase domain	PFAM	PF01575	MaoC_dehydratas	coord: 37..128 e-value: 2.2E-17 score: 62.8
IPR029069	HotDog domain superfamily	SUPERFAMILY	54637	Thioesterase/thiol ester dehydrase-isomerase	coord: 27..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0013517.1	Tan0013517.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0045145	single-stranded DNA 5'-3' exodeoxyribonuclease activity