Tan0013435 (gene) Snake gourd v1

Overview


Name	Tan0013435
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	isoflavone reductase homolog
Location	LG07: 72002985 .. 72006413 (-)
RNA-Seq Expression	Tan0013435
Synteny	Tan0013435

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAAAGAGGGAAAATGAGAGTATTGGTGGTTGGAGGAACTGGTTATTTGGGAAGGAGGATTGTGAAAGCCAGCCTTTTGCAGGGCCATCAAACGTATGTGGTTCAAAGGCCTGAGATTGGCCTTGACATTGACAAGCTTCAATTGCTTCTTTCTTTCAAGAAACAGGGAGCTCTGCTTGTTCCGGCCTCCTTCTCCGATCTTCAAAGCCTCGTCGACGCTGTCAAACGCGTCGACGTCGTGATTTCGGCCATGTCCGGCGTCCATTTCCGAAGCCATAACCTTCTGATGCAACTTAAGCTTGTTGAGGCCATTCAAGTTGCAGGGAATGTCAAGGTTTTCATTTCATCTCTTTCCATTAATATACTTTGCATTTTAATTTTAGTTGATGATGTTTCATGTTTAAGTTTTATTTAGCTCCTCAACTTTAAAAAATTCTTAAATGATTTTGTTTTTGTTGTCCTTGAATTTTTTAAAATGTTCAATTGATTAAATTTTTAATTTTGTGTCTACTGCGTCTTTCTCCTATTCAACATTTTTTAAAATTTCTAGATATAATTGATCGATTAATTTTTCAAATTTTTGAATATGTCAAAGATTTATTAGATATATAAAATTAGCATTTCAAGACACTTTTCATAGTTTAGCGATCAAATAAACACAAACTAAAAAAATTAGGGACTAAACTTGTAATTTAAACAATGACATATCTTTCTGAGTTGATCGGAGGTTGTCTTTACTGTTTAATTATTATTATTGTTATTATTTACTTAATAATTATAAGTTTTTGTTCCTCAACTTTTAATTTTTTTACTAATAGGTCTATTTTATAGTTTTATAAACTTTTAGTTTCATGTTTAATAGCTCTTGAACTTCAAGAATATCTAATAGGAATTTAAATTTTCAATTCTATATTTAATTGGTCATCGACCTATTTGACATTTTTAAACTTTATAGTAACTCAAAATTAAAAGTTTAGATTTCTACCAGAGACAAATTTATATATTTTTTTTGTATGATCTATTTTTTTAAAAAAAAACAAAAATGTTGAATATGTGATGACAACTATTAGACCTTCGGACATGATTAGACACAAATTTAAAAGTTTGAGATTAAACAGATTTCTCTCCATCTCAATCTCAATCTAGAGGTTGTTCTTTCTCTTTGTTTATAGTTCCAAAAGCAATTATCTACCTACTCAACAATTATCTCTCTTTCTGTTTCAAAAAAAAAAAAAAAACAATTATCTCTCTTTGAAAAATTAAGAAAGCAGTTCTAATATATTAATATAGTATGCTATTACTATTAGAGCATATTTCTTTTTTATATATATTATTAATGAAATAATATGTCAGCCATAAATAAATTGAACAATTATTTGATTGGACTGACCTAACTGTTTAATGGTTAAAATATCTCTATCTCTTTCAAAGGGCTAGAAATTTCAATCTCAACCATATGTTTTTTATTAACAAAAGATTCTCATTCGTTGGGGGTTTTTTTTTTTTTCCCTTAAATTTGGTGGGTACAAATATAGAGATTTTTGCCATCAGAATATGGGATAGATCCAGCAAGAATGGGAGATGCACTTGAACCAGGAAGAGTGTCATTTGATGAGAAAATGAGAATAAGAAAAGCAATAGAAGAAGCCAATATTCCTTTCACCTACGTCTCTGCTAACTGCTTTGCCGGTTACTTTGCCGGTAGCCTGTCTCAAATGTTCACTCTGGTCCCTCCAACCCACCAAGTTACCCTTTATGGGGATGGTGATGTTAAAGGTAACCCAATATGTTTAAATAATACTTTTTATTTTTTTATTTTTTTTTTCAGTTCAATAAGGTATAAAATGGGACGATTTAAACATCTATCTTCTTGATCGATGATATATATTTTAATCAGCTTTGGCTCAATTTTGAAGATATGCTTTCAAATGCTTTCAAAATGTCCATTTTTAATTATTGTTTGTTGAAACGTTTTGAGCCTGTTTAGGCCACTGAGTGGTAGTTATAATAATATGTAAAATTAAATAACTTTGCACTTAACCATAGACTATTATAATCCTAACAATTTTACATCCAAAACATATATTAATTATATAATTTCATAGATTATTATAACCTGTCCAACGCCTAAATAGCTCAATATGTGGAAGAAAAAATTTTGAACCTTAACATTTTTGTTGAAATACAATACCTTAATCAATTGAATTGTGCATAAATTAGCTTGAAACGTCTATTAAAATATGGAAAGAGAGTTGTATAATTTTATTTTTCTGTTTGTATTGAATGTTTCAAAATTTTCAACCTATAATGAAATAATAACACGTTACATTTGACGTGACGAAATTTACAGTGATCTATGTGGATGAAGATGACATTGCATCATACGTCGTGAAAACGACAGATGATCCTCGAACTCTGAACAAAACAGTGTACATTAAGCCTCCAGAAAACATTCTAACTCAAAGAGAGTTGATTGAGAAATGGGAAAAGCTTTCTGGAAAACAATTACAAAAGAACACTGTTTCTGCTCAAGATTTTCTCGCCAAGATGAAAGGTGATATTAGGTTAGGTCATGTCATGTCATCTAAAAGAGAAAATGTTGAATTTTTCTTTCAATTTATTTTCTTAAAAAATTTGAAAGAGTTAAAGAAGTTCTTTGGCTTAGTACTTCACCTAAATTTATACTAACTCCCTAAGTTTCATATAACTTTATCGACACCCAATTTTAAAATAGACCTACAAAAAAGGATATGATTGAAAAATTGTAAAAGGTTAGGGATCAAATTGAAACTTTTAGATAATAAAAACTAAATTGAAAAACCTTCCAACATTAGGGGGCTACTAGAGCATAAATTATATATGAACTATTTTTTAAAAGGAAAAGTAATTTCATTTGTGTTCTAAAGAATTAGGAACAAAAGTCCATAAAAACCAAATTTGTTTCCAAATATGAGCTCCTAAGGGATGACTCCATCGGGGAAAGCCAATGAACTTAATAAGAAAACCTCGATGTCTCTAAAGTTTGGACTTTAGGGTTCAAACATTTTAAAAGATTTTGTATACAAATATGGAAACTTCTACAAATGATAAAAAATGAAAAACAAAGAACATTACCAAACCAAACAGGTCTATTTTACAAGAAAAAAAAAAGAATAGAAAACATAAACATTATCATAGCATACTTTAGTGAAGGCCAAAGTTGTTATGATATTTCAACCTTGTCAAATTCTTCATTCGTATGAACAAAAGCAAGTTGCATGCAGATGGGGACTTAGCATGGAAGGTTGGAGTGGGACATTTTTACCATATATTCTTCGAAGGCTGCTTGACAAACTTTGAAATAGGAGAAGGAGAAGAAGCTTCAAAGCTTTATCCAGAAGTCAACTATACTCGCATGGATGATTACCTGAAGATATTTTTATGA

mRNA sequence

ATGGCGGAAAGAGGGAAAATGAGAGTATTGGTGGTTGGAGGAACTGGTTATTTGGGAAGGAGGATTGTGAAAGCCAGCCTTTTGCAGGGCCATCAAACGTATGTGGTTCAAAGGCCTGAGATTGGCCTTGACATTGACAAGCTTCAATTGCTTCTTTCTTTCAAGAAACAGGGAGCTCTGCTTGTTCCGGCCTCCTTCTCCGATCTTCAAAGCCTCGTCGACGCTGTCAAACGCGTCGACGTCGTGATTTCGGCCATGTCCGGCGTCCATTTCCGAAGCCATAACCTTCTGATGCAACTTAAGCTTGTTGAGGCCATTCAAGTTGCAGGGAATGTCAAGAGATTTTTGCCATCAGAATATGGGATAGATCCAGCAAGAATGGGAGATGCACTTGAACCAGGAAGAGTGTCATTTGATGAGAAAATGAGAATAAGAAAAGCAATAGAAGAAGCCAATATTCCTTTCACCTACGTCTCTGCTAACTGCTTTGCCGGTTACTTTGCCGGTAGCCTGTCTCAAATGTTCACTCTGGTCCCTCCAACCCACCAAGTTACCCTTTATGGGGATGGTGATGTTAAAGTGATCTATGTGGATGAAGATGACATTGCATCATACGTCGTGAAAACGACAGATGATCCTCGAACTCTGAACAAAACAGTGTACATTAAGCCTCCAGAAAACATTCTAACTCAAAGAGAGTTGATTGAGAAATGGGAAAAGCTTTCTGGAAAACAATTACAAAAGAACACTGTTTCTGCTCAAGATTTTCTCGCCAAGATGAAAGATGGGGACTTAGCATGGAAGGTTGGAGTGGGACATTTTTACCATATATTCTTCGAAGGCTGCTTGACAAACTTTGAAATAGGAGAAGGAGAAGAAGCTTCAAAGCTTTATCCAGAAGTCAACTATACTCGCATGGATGATTACCTGAAGATATTTTTATGA

Coding sequence (CDS)

ATGGCGGAAAGAGGGAAAATGAGAGTATTGGTGGTTGGAGGAACTGGTTATTTGGGAAGGAGGATTGTGAAAGCCAGCCTTTTGCAGGGCCATCAAACGTATGTGGTTCAAAGGCCTGAGATTGGCCTTGACATTGACAAGCTTCAATTGCTTCTTTCTTTCAAGAAACAGGGAGCTCTGCTTGTTCCGGCCTCCTTCTCCGATCTTCAAAGCCTCGTCGACGCTGTCAAACGCGTCGACGTCGTGATTTCGGCCATGTCCGGCGTCCATTTCCGAAGCCATAACCTTCTGATGCAACTTAAGCTTGTTGAGGCCATTCAAGTTGCAGGGAATGTCAAGAGATTTTTGCCATCAGAATATGGGATAGATCCAGCAAGAATGGGAGATGCACTTGAACCAGGAAGAGTGTCATTTGATGAGAAAATGAGAATAAGAAAAGCAATAGAAGAAGCCAATATTCCTTTCACCTACGTCTCTGCTAACTGCTTTGCCGGTTACTTTGCCGGTAGCCTGTCTCAAATGTTCACTCTGGTCCCTCCAACCCACCAAGTTACCCTTTATGGGGATGGTGATGTTAAAGTGATCTATGTGGATGAAGATGACATTGCATCATACGTCGTGAAAACGACAGATGATCCTCGAACTCTGAACAAAACAGTGTACATTAAGCCTCCAGAAAACATTCTAACTCAAAGAGAGTTGATTGAGAAATGGGAAAAGCTTTCTGGAAAACAATTACAAAAGAACACTGTTTCTGCTCAAGATTTTCTCGCCAAGATGAAAGATGGGGACTTAGCATGGAAGGTTGGAGTGGGACATTTTTACCATATATTCTTCGAAGGCTGCTTGACAAACTTTGAAATAGGAGAAGGAGAAGAAGCTTCAAAGCTTTATCCAGAAGTCAACTATACTCGCATGGATGATTACCTGAAGATATTTTTATGA

Protein sequence

MAERGKMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPASFSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPARMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVTLYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQLQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEGEEASKLYPEVNYTRMDDYLKIFL

Homology

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BLAST of Tan0013435 vs. ExPASy Swiss-Prot
Match: P52581 (Isoflavone reductase homolog OS=Lupinus albus OX=3870 PE=2 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 1.4e-138
Identity = 239/311 (76.85%), Postives = 281/311 (90.35%), Query Frame = 0


Query: 5   GKMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPA 64
           GK +VLVVGGTGY+GRRIVKASL  GH+T+++QRPEIGLDI+KLQ+LLSFKKQGA+LV A
Sbjct: 2   GKSKVLVVGGTGYVGRRIVKASLEHGHETFILQRPEIGLDIEKLQILLSFKKQGAILVEA 61

Query: 65  SFSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDP 124
           SFSD +SLVDAVK VDVVI  MSGVHFRSHNLL QLKLVEAI+ AGN+KRFLPSE+G+DP
Sbjct: 62  SFSDHKSLVDAVKLVDVVICTMSGVHFRSHNLLTQLKLVEAIKDAGNIKRFLPSEFGMDP 121

Query: 125 ARMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQV 184
           A MG ALEPGRV+FDEKM +RKAIEEANIPFTY+SANCFAGYFAG+LSQM TL+PP  +V
Sbjct: 122 ALMGHALEPGRVTFDEKMTVRKAIEEANIPFTYISANCFAGYFAGNLSQMKTLLPPRDKV 181

Query: 185 TLYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGK 244
            LYGDG+VK +Y+DEDD+A+Y +KT DDPRTLNKTVY++PPENILT +ELIEKWE+L GK
Sbjct: 182 LLYGDGNVKPVYMDEDDVATYTIKTIDDPRTLNKTVYLRPPENILTHKELIEKWEELIGK 241

Query: 245 QLQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGE-GEEASKLYPEVNY 304
           QL+KN++S +DFL+ +K  D A +VGVGHFYHIF+EGCLTNFEIGE GEEAS+LYPEVNY
Sbjct: 242 QLEKNSISEKDFLSTLKGLDFASQVGVGHFYHIFYEGCLTNFEIGENGEEASELYPEVNY 301

Query: 305 TRMDDYLKIFL 315
           TRMD YLK+++
Sbjct: 302 TRMDQYLKVYV 312

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BLAST of Tan0013435 vs. ExPASy Swiss-Prot
Match: Q9FVQ6 (Pinoresinol reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PRR1 PE=1 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 3.8e-123
Identity = 209/309 (67.64%), Postives = 260/309 (84.14%), Query Frame = 0


Query: 6   KMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPAS 65
           K RVLVVG TGY+G+RIV+A L +GH+TYV+QRPEIGL+I+K+QL LSFKK GA +V  S
Sbjct: 9   KTRVLVVGATGYIGKRIVRACLAEGHETYVLQRPEIGLEIEKVQLFLSFKKLGARIVEGS 68

Query: 66  FSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPA 125
           FSD QSLV AVK VDVV+SAMSGVHFRSHN+L+QLKLVEAI+ AGNVKRFLPSE+G+DP 
Sbjct: 69  FSDHQSLVSAVKLVDVVVSAMSGVHFRSHNILVQLKLVEAIKEAGNVKRFLPSEFGMDPP 128

Query: 126 RMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVT 185
           RMG AL PGR +FD+KM +R+AIE A IP+TYV   CFA YFAG+LSQM TL+PP  +V 
Sbjct: 129 RMGHALPPGRETFDQKMEVRQAIEAAGIPYTYVVGACFAAYFAGNLSQMVTLLPPKEKVN 188

Query: 186 LYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQ 245
           +YGDG+VKV++ DEDDIA Y  KT +DPRTLNKTV I+PP+N+LTQ EL++ WEKL+GK+
Sbjct: 189 IYGDGNVKVVFADEDDIAKYTAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKE 248

Query: 246 LQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEGEEASKLYPEVNYTR 305
           L+K  ++AQDFLA ++  ++  + G+GHFYHIF+EGCLT+ E+GE EEAS LYP+V Y R
Sbjct: 249 LEKTNIAAQDFLANIEQMEIPHQAGIGHFYHIFYEGCLTDHEVGEDEEASSLYPDVKYKR 308

Query: 306 MDDYLKIFL 315
           MDDYL++FL
Sbjct: 309 MDDYLRMFL 317

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BLAST of Tan0013435 vs. ExPASy Swiss-Prot
Match: A3R052 (Bifunctional pinoresinol-lariciresinol reductase OS=Linum perenne OX=35941 GN=PLR_Lp1 PE=1 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 3.2e-122
Identity = 216/310 (69.68%), Postives = 261/310 (84.19%), Query Frame = 0


Query: 9   VLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPASFSD 68
           VLVVGGTGY+G+RIV ASL  GH TYV++RP  GLDI+KLQLLLSFKK+GA LV ASFSD
Sbjct: 6   VLVVGGTGYIGKRIVSASLYLGHDTYVLKRPGTGLDIEKLQLLLSFKKRGAHLVEASFSD 65

Query: 69  LQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPARMG 128
             SLV AV+ VDVVI  MSGVHFRSHN+L+QLKLVEAI+ AGNVKRF+PSE+G+DPARMG
Sbjct: 66  HDSLVRAVRLVDVVICTMSGVHFRSHNILLQLKLVEAIKEAGNVKRFIPSEFGMDPARMG 125

Query: 129 DALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVTLYG 188
            A+EPGR +FD+KM +RKAIEEANIP TY+SANCFAGYF G+LSQ+ TL PP+ +V +YG
Sbjct: 126 QAMEPGRETFDQKMVVRKAIEEANIPHTYISANCFAGYFVGNLSQLGTLTPPSDKVIIYG 185

Query: 189 DGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQLQK 248
           DG+VKV+YVDEDD+A Y +K  +D RT+NKTVY++PPEN+++QREL+  WEKLSG QL+K
Sbjct: 186 DGNVKVVYVDEDDVAKYTIKAIEDDRTVNKTVYLRPPENMMSQRELVAVWEKLSGNQLEK 245

Query: 249 NTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEI----GEGEEASKLYPEVNYT 308
             +  QDFLA M+   +A + G+GHFYHIF+EGCLTNFEI    GE EEAS+LYPEV YT
Sbjct: 246 IELPPQDFLALMEGTTVAEQAGIGHFYHIFYEGCLTNFEINAENGE-EEASRLYPEVEYT 305

Query: 309 RMDDYLKIFL 315
           R+ DYLKI+L
Sbjct: 306 RVHDYLKIYL 314

[Close]

BLAST of Tan0013435 vs. ExPASy Swiss-Prot
Match: P0DKC8 (Bifunctional pinoresinol-lariciresinol reductase OS=Linum usitatissimum OX=4006 PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 2.5e-119
Identity = 211/313 (67.41%), Postives = 259/313 (82.75%), Query Frame = 0


Query: 5   GKMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPA 64
           G+ RVLVVGGTGY+G+RIVKAS+  GH TYV++RPE GLDI+K QLLLSFKKQGA LV A
Sbjct: 2   GRCRVLVVGGTGYIGKRIVKASIEHGHDTYVLKRPETGLDIEKFQLLLSFKKQGAHLVEA 61

Query: 65  SFSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDP 124
           SFSD +SLV AVK VDVVI  +SG H RS  LL+QLKLVEAI+ AGNVKRF+PSE+G+DP
Sbjct: 62  SFSDHESLVRAVKLVDVVICTVSGAHSRS--LLLQLKLVEAIKEAGNVKRFIPSEFGMDP 121

Query: 125 ARMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQV 184
           ARMGDALEPGR +FD KM +RKAIE+ANIP TY+SANCF GYF G+LSQ+  L PP+ +V
Sbjct: 122 ARMGDALEPGRETFDLKMVVRKAIEDANIPHTYISANCFGGYFVGNLSQLGPLTPPSDKV 181

Query: 185 TLYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGK 244
           T+YGDG+VKV+Y+DEDD+A+Y + T +D RTLNKT+Y +PPEN++T R+L+E WEKLSG 
Sbjct: 182 TIYGDGNVKVVYMDEDDVATYTIMTIEDDRTLNKTMYFRPPENVITHRQLVETWEKLSGN 241

Query: 245 QLQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEGE---EASKLYPEV 304
           QLQK  +S+QDFLA M+  D+A ++ +GH YHI++EGCLTNF+I   +   EAS LYPEV
Sbjct: 242 QLQKTELSSQDFLALMEGKDVAEQIVIGHLYHIYYEGCLTNFDIDADQDQVEASSLYPEV 301

Query: 305 NYTRMDDYLKIFL 315
            YTRM DYL I+L
Sbjct: 302 EYTRMKDYLMIYL 312

[Close]

BLAST of Tan0013435 vs. ExPASy Swiss-Prot
Match: Q4R0H9 (Bifunctional pinoresinol-lariciresinol reductase 1 OS=Linum usitatissimum OX=4006 GN=PLR_Lu1 PE=1 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 4.3e-119
Identity = 211/313 (67.41%), Postives = 259/313 (82.75%), Query Frame = 0


Query: 5   GKMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPA 64
           G+ RVLVVGGTGY+G+RIVKAS+  GH TYV++RPE GLDI+K QLLLSFKKQGA LV A
Sbjct: 2   GRCRVLVVGGTGYIGKRIVKASIEHGHDTYVLKRPETGLDIEKFQLLLSFKKQGAHLVEA 61

Query: 65  SFSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDP 124
           SFSD +SLV AVK VDVVI  +SG H RS  LL+QLKLVEAI+ AGNVKRF+PSE+G+DP
Sbjct: 62  SFSDHESLVRAVKLVDVVICTVSGAHSRS--LLLQLKLVEAIKEAGNVKRFIPSEFGMDP 121

Query: 125 ARMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQV 184
           ARMGDALEPGR +FD KM +RKAIE+ANIP TY+SANCF GYF G+LSQ+  L PP+ +V
Sbjct: 122 ARMGDALEPGRETFDLKMVVRKAIEDANIPHTYISANCFGGYFVGNLSQLGPLTPPSDKV 181

Query: 185 TLYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGK 244
           T+YGDG+VKV+Y+DEDD+A+Y + T +D RTLNKT+Y++PPEN++T R+L+E WEKLSG 
Sbjct: 182 TIYGDGNVKVVYMDEDDVATYTIMTIEDDRTLNKTMYLRPPENVITHRQLVETWEKLSGN 241

Query: 245 QLQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEGE---EASKLYPEV 304
           QLQK  +S+QDFLA M+  D+A +V +GH YHI++EGCLTNF+I   +   EAS LYPEV
Sbjct: 242 QLQKTELSSQDFLALMEGKDVAEQVVIGHLYHIYYEGCLTNFDIDAAQDQVEASSLYPEV 301

Query: 305 NYTRMDDYLKIFL 315
            Y RM DYL I+L
Sbjct: 302 EYIRMKDYLMIYL 312

[Close]

BLAST of Tan0013435 vs. NCBI nr
Match: XP_022967298.1 (isoflavone reductase homolog [Cucurbita maxima])

HSP 1 Score: 543.1 bits (1398), Expect = 1.6e-150
Identity = 269/309 (87.06%), Postives = 294/309 (95.15%), Query Frame = 0


Query: 7   MRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPASF 66
           M+VLVVGGTGYLG+RIVKASLL+GHQTYVVQRPEIGLDI+KLQLLLSFK+QGA+LVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 67  SDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPAR 126
           SD QSLVDAVKRVDVVISAMSGVHFRSHNLL+QLKLVEAIQVAGNVKRFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 127 MGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVTL 186
           M DALEPGR +FD+KM IRKAIEEANIPFTYVSANCFAGYFAGSLSQM +LVPPTHQVTL
Sbjct: 121 MRDALEPGRETFDQKMTIRKAIEEANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVTL 180

Query: 187 YGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQL 246
           YGDG+VKV+YVDEDDIA+Y+VK   DPRT+NKTVYI+PP+NIL+QRELI+KWE LSGK L
Sbjct: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240

Query: 247 QKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEG-EEASKLYPEVNYTR 306
           +KN VSAQDFLA+MKD D+A + GVGHFYH+FFEGCLTNFEIGEG EEAS+LYPEVNYTR
Sbjct: 241 EKNRVSAQDFLARMKDLDIAQQAGVGHFYHLFFEGCLTNFEIGEGEEEASRLYPEVNYTR 300

Query: 307 MDDYLKIFL 315
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

[Close]

BLAST of Tan0013435 vs. NCBI nr
Match: XP_023511514.1 (isoflavone reductase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 541.6 bits (1394), Expect = 4.5e-150
Identity = 269/309 (87.06%), Postives = 290/309 (93.85%), Query Frame = 0


Query: 7   MRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPASF 66
           M+VLVVGGTGYLG+RIVKASLL+GHQTYVVQRPEIGLDI+KLQLLLSFK+QGA+LVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 67  SDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPAR 126
           SD  SLVDAVKRVDVVISAMSGVHFRSHNLL+QLKLVEAIQVAGNVKRFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 127 MGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVTL 186
           M DALEPGR +FD+KM IRKAIEEANIPFTYVSANCFAGYFAGSLSQM TLVPPTHQVTL
Sbjct: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180

Query: 187 YGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQL 246
           YGDG+VK +YVDEDDIA+Y+VK   DPRT+NKTVYI+PP NIL+QRELI+KWE LSGK L
Sbjct: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240

Query: 247 QKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEG-EEASKLYPEVNYTR 306
           +KN VSAQDFL +MKD D+A + GVGHFYHIFFEGCLTNFEIGEG EEASKLYPEVNYTR
Sbjct: 241 EKNRVSAQDFLTRMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300

Query: 307 MDDYLKIFL 315
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

[Close]

BLAST of Tan0013435 vs. NCBI nr
Match: XP_038887505.1 (isoflavone reductase homolog [Benincasa hispida])

HSP 1 Score: 541.6 bits (1394), Expect = 4.5e-150
Identity = 267/309 (86.41%), Postives = 294/309 (95.15%), Query Frame = 0


Query: 7   MRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPASF 66
           MRVLVVGGTGYLG+RIVKASLL+GHQT+V+QRPEIGLDI+KLQLLLSFKKQGA+LVPASF
Sbjct: 17  MRVLVVGGTGYLGKRIVKASLLEGHQTFVIQRPEIGLDIEKLQLLLSFKKQGAVLVPASF 76

Query: 67  SDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPAR 126
           SD QSLVDAVKRVDVVISAMSGVHFRSHNLL+QLKLVEAIQVAGN+KR +PSEYGIDPAR
Sbjct: 77  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNIKRLVPSEYGIDPAR 136

Query: 127 MGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVTL 186
           MG+ALEPGR +FD+KM IRKAIEEANIPFTY+SANCFAGYFAG+LSQM TLVPPTHQVTL
Sbjct: 137 MGNALEPGRETFDQKMVIRKAIEEANIPFTYISANCFAGYFAGNLSQMLTLVPPTHQVTL 196

Query: 187 YGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQL 246
           YGDG+VKVIYVDEDDIA+Y+VK   DPRT NKTVYI+PP+NIL+QRELIEKWEKLSGK L
Sbjct: 197 YGDGNVKVIYVDEDDIATYIVKAATDPRTENKTVYIRPPQNILSQRELIEKWEKLSGKVL 256

Query: 247 QKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEG-EEASKLYPEVNYTR 306
           +K +VSAQDFLA+MKD D+A + GVGHFYHIFFEGCLTNFEIGEG EEASKL+PEVNYTR
Sbjct: 257 EKKSVSAQDFLARMKDMDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLFPEVNYTR 316

Query: 307 MDDYLKIFL 315
           MDDYLKIF+
Sbjct: 317 MDDYLKIFI 325

[Close]

BLAST of Tan0013435 vs. NCBI nr
Match: KAG6571626.1 (hypothetical protein SDJN03_28354, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 541.2 bits (1393), Expect = 5.9e-150
Identity = 268/309 (86.73%), Postives = 294/309 (95.15%), Query Frame = 0


Query: 7   MRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPASF 66
           M+VLVVGGTGYLG+RIVKASLL+GHQTYVVQRPEIGLDI+KLQLLLSFK+QGA+LVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 67  SDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPAR 126
           SD QSLVDAVKRVDVVISAMSGVHFRSHNLL+QLKLVEAIQVAGNV+RFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120

Query: 127 MGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVTL 186
           M DALEPGR +FD+KM IRKAIE+ANIPFTYVSANCFAGYFAGSLSQM +LVPPTHQV L
Sbjct: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180

Query: 187 YGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQL 246
           YGDG+VKV+YVDEDDIA+Y+VK   DPRT+NKTVYI+PP+NIL+QRELI+KWE LSGK L
Sbjct: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240

Query: 247 QKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEG-EEASKLYPEVNYTR 306
           +KN+VSAQDFLA+MKD D+A + GVGHFYHIFFEGCLTNFEIGEG EEASKLYPEVNYTR
Sbjct: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300

Query: 307 MDDYLKIFL 315
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

[Close]

BLAST of Tan0013435 vs. NCBI nr
Match: XP_022159309.1 (isoflavone reductase homolog [Momordica charantia])

HSP 1 Score: 533.9 bits (1374), Expect = 9.5e-148
Identity = 262/311 (84.24%), Postives = 290/311 (93.25%), Query Frame = 0


Query: 5   GKMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPA 64
           GK RVLVVGGTGYLG+RIV ASL QGHQTYV+QRPEIGLDI+KLQLLLSFK+QGA+L+PA
Sbjct: 3   GKSRVLVVGGTGYLGKRIVAASLAQGHQTYVIQRPEIGLDIEKLQLLLSFKRQGAVLLPA 62

Query: 65  SFSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDP 124
           SFSD QSLVDAVKRVDVVISA+SGVHFRSH+LL+QLKL+EAI+VAGN+KRF PSEYGIDP
Sbjct: 63  SFSDFQSLVDAVKRVDVVISAISGVHFRSHSLLVQLKLIEAIKVAGNIKRFFPSEYGIDP 122

Query: 125 ARMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQV 184
           ARMG+ALEPGR +FD+KM +RKAIEEA IPFTYVSANCFAGYFAG+LSQM TL+PPTHQV
Sbjct: 123 ARMGNALEPGRETFDQKMAVRKAIEEAKIPFTYVSANCFAGYFAGNLSQMQTLIPPTHQV 182

Query: 185 TLYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGK 244
           TLYGDG+VKVIYVDEDD+ASY+VK  DDPRTLNKT+YI+PP NILTQ ELI KWEKL GK
Sbjct: 183 TLYGDGNVKVIYVDEDDVASYIVKAVDDPRTLNKTLYIRPPHNILTQTELIGKWEKLFGK 242

Query: 245 QLQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGE-GEEASKLYPEVNY 304
           QLQKNT+SA+DFLA MKD D A + G+GHFYHIFFEGCLTNFEIGE GEEAS+LYPEVNY
Sbjct: 243 QLQKNTISAEDFLATMKDLDSAQQAGLGHFYHIFFEGCLTNFEIGEGGEEASQLYPEVNY 302

Query: 305 TRMDDYLKIFL 315
           TRMDDYLKIFL
Sbjct: 303 TRMDDYLKIFL 313

[Close]

BLAST of Tan0013435 vs. ExPASy TrEMBL
Match: A0A6J1HUP1 (isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466861 PE=4 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 7.6e-151
Identity = 269/309 (87.06%), Postives = 294/309 (95.15%), Query Frame = 0


Query: 7   MRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPASF 66
           M+VLVVGGTGYLG+RIVKASLL+GHQTYVVQRPEIGLDI+KLQLLLSFK+QGA+LVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 67  SDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPAR 126
           SD QSLVDAVKRVDVVISAMSGVHFRSHNLL+QLKLVEAIQVAGNVKRFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 127 MGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVTL 186
           M DALEPGR +FD+KM IRKAIEEANIPFTYVSANCFAGYFAGSLSQM +LVPPTHQVTL
Sbjct: 121 MRDALEPGRETFDQKMTIRKAIEEANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVTL 180

Query: 187 YGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQL 246
           YGDG+VKV+YVDEDDIA+Y+VK   DPRT+NKTVYI+PP+NIL+QRELI+KWE LSGK L
Sbjct: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240

Query: 247 QKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEG-EEASKLYPEVNYTR 306
           +KN VSAQDFLA+MKD D+A + GVGHFYH+FFEGCLTNFEIGEG EEAS+LYPEVNYTR
Sbjct: 241 EKNRVSAQDFLARMKDLDIAQQAGVGHFYHLFFEGCLTNFEIGEGEEEASRLYPEVNYTR 300

Query: 307 MDDYLKIFL 315
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

[Close]

BLAST of Tan0013435 vs. ExPASy TrEMBL
Match: A0A6J1E225 (isoflavone reductase homolog OS=Momordica charantia OX=3673 GN=LOC111025718 PE=4 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 4.6e-148
Identity = 262/311 (84.24%), Postives = 290/311 (93.25%), Query Frame = 0


Query: 5   GKMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPA 64
           GK RVLVVGGTGYLG+RIV ASL QGHQTYV+QRPEIGLDI+KLQLLLSFK+QGA+L+PA
Sbjct: 3   GKSRVLVVGGTGYLGKRIVAASLAQGHQTYVIQRPEIGLDIEKLQLLLSFKRQGAVLLPA 62

Query: 65  SFSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDP 124
           SFSD QSLVDAVKRVDVVISA+SGVHFRSH+LL+QLKL+EAI+VAGN+KRF PSEYGIDP
Sbjct: 63  SFSDFQSLVDAVKRVDVVISAISGVHFRSHSLLVQLKLIEAIKVAGNIKRFFPSEYGIDP 122

Query: 125 ARMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQV 184
           ARMG+ALEPGR +FD+KM +RKAIEEA IPFTYVSANCFAGYFAG+LSQM TL+PPTHQV
Sbjct: 123 ARMGNALEPGRETFDQKMAVRKAIEEAKIPFTYVSANCFAGYFAGNLSQMQTLIPPTHQV 182

Query: 185 TLYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGK 244
           TLYGDG+VKVIYVDEDD+ASY+VK  DDPRTLNKT+YI+PP NILTQ ELI KWEKL GK
Sbjct: 183 TLYGDGNVKVIYVDEDDVASYIVKAVDDPRTLNKTLYIRPPHNILTQTELIGKWEKLFGK 242

Query: 245 QLQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGE-GEEASKLYPEVNY 304
           QLQKNT+SA+DFLA MKD D A + G+GHFYHIFFEGCLTNFEIGE GEEAS+LYPEVNY
Sbjct: 243 QLQKNTISAEDFLATMKDLDSAQQAGLGHFYHIFFEGCLTNFEIGEGGEEASQLYPEVNY 302

Query: 305 TRMDDYLKIFL 315
           TRMDDYLKIFL
Sbjct: 303 TRMDDYLKIFL 313

[Close]

BLAST of Tan0013435 vs. ExPASy TrEMBL
Match: A0A6J1HLT4 (isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464122 PE=4 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 4.6e-148
Identity = 265/309 (85.76%), Postives = 289/309 (93.53%), Query Frame = 0


Query: 7   MRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPASF 66
           M+VLVVGGTGYLG+RIVKASLL+GHQTYVVQRPEIGLDI+KLQLLLSFK+QGA+LVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 67  SDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPAR 126
           SD  SLVDAVKRVDVVISAMSGVHFRSHNLL+QLKLVEAIQVAGNVKRFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 127 MGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVTL 186
           M DALEPGR +FD+KM +RKAIEEANIPFTYVSANCFAGYFAGSLSQM TL PPTHQVTL
Sbjct: 121 MRDALEPGRETFDQKMIVRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLAPPTHQVTL 180

Query: 187 YGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQL 246
           YGDG+VK +YVDEDDIA+Y+VK   DPRT+NKTVYI+P +NIL+QRELI+KWE LS K L
Sbjct: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPRDNILSQRELIQKWEALSEKVL 240

Query: 247 QKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEG-EEASKLYPEVNYTR 306
           +KN+VSAQDFL +MKD D+A + GVGHFYHIFFEGCLTNFEIGEG EEASKLYPEVNYTR
Sbjct: 241 EKNSVSAQDFLTRMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPEVNYTR 300

Query: 307 MDDYLKIFL 315
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

[Close]

BLAST of Tan0013435 vs. ExPASy TrEMBL
Match: A0A6J1HJH3 (isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464118 PE=4 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.7e-147
Identity = 257/308 (83.44%), Postives = 288/308 (93.51%), Query Frame = 0


Query: 7   MRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPASF 66
           M+VLVVGG+GYLG+RIVKASLL+GHQTYVVQRPEIGLDI+KLQLLLSFK+QGA+LVPASF
Sbjct: 1   MKVLVVGGSGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 67  SDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPAR 126
           SD  SLVDAVKRVDVVISAMSGVHFRSHNLL+QLKLVEAIQVAGN+KRFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNIKRFLPSEYGIDPAR 120

Query: 127 MGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVTL 186
           M DALEPGR +FD+KM +RKAIEEANIPFTYVSANCFAGYFAGSLS+M TLVPPTHQVTL
Sbjct: 121 MKDALEPGRETFDQKMTVRKAIEEANIPFTYVSANCFAGYFAGSLSEMHTLVPPTHQVTL 180

Query: 187 YGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQL 246
           YGDG+VK +YVDEDDIA+YVVK   DPRT+NKTVYI+PP NIL+QRE++EKWE LSGK L
Sbjct: 181 YGDGNVKAVYVDEDDIATYVVKAATDPRTMNKTVYIRPPHNILSQREMVEKWEALSGKVL 240

Query: 247 QKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEGEEASKLYPEVNYTRM 306
           +K ++SAQDFLA+MKD DL  + G+GHFYHIFF+GC+TNFE+ E EEASKLYPEVNYTRM
Sbjct: 241 EKKSISAQDFLARMKDLDLGQRAGLGHFYHIFFDGCMTNFEVREDEEASKLYPEVNYTRM 300

Query: 307 DDYLKIFL 315
           DDYLKI+L
Sbjct: 301 DDYLKIYL 308

[Close]

BLAST of Tan0013435 vs. ExPASy TrEMBL
Match: A0A6J1HWD5 (isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466862 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.3e-147
Identity = 258/308 (83.77%), Postives = 288/308 (93.51%), Query Frame = 0


Query: 7   MRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPASF 66
           M+VLVVGG+GYLG+RIVKASLL+GHQTYVVQRPEIGLDI+KLQLLLSFK+QGA+LVPASF
Sbjct: 1   MKVLVVGGSGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 67  SDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPAR 126
           SD QSLVDAVKRVDVVISAMSGVHFRSHNLL+QLKLVEAIQVAGN+KRFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNIKRFLPSEYGIDPAR 120

Query: 127 MGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVTL 186
           M DALEPGR +FD+KM +RKAIEEANIPFTYVSANCFAGYFAGSLS+M TLVPPTHQVTL
Sbjct: 121 MKDALEPGRETFDQKMIVRKAIEEANIPFTYVSANCFAGYFAGSLSEMHTLVPPTHQVTL 180

Query: 187 YGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQL 246
           YGDG+VK +YVDEDDIA+YVVK   DPRT+NKTVYI+PP NIL+QRE++EKWE LSGK L
Sbjct: 181 YGDGNVKAVYVDEDDIATYVVKAATDPRTMNKTVYIRPPHNILSQREMVEKWEALSGKVL 240

Query: 247 QKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEGEEASKLYPEVNYTRM 306
           +K +VS QDFLA+MKD DL  + G+GHFYHIFF+GC+TNFE+ E EEASKLYPEVNYTRM
Sbjct: 241 EKKSVSPQDFLARMKDLDLGQRAGLGHFYHIFFDGCMTNFEVREDEEASKLYPEVNYTRM 300

Query: 307 DDYLKIFL 315
           DDYLKI+L
Sbjct: 301 DDYLKIYL 308

[Close]

BLAST of Tan0013435 vs. TAIR 10
Match: AT1G32100.1 (pinoresinol reductase 1 )

HSP 1 Score: 442.6 bits (1137), Expect = 2.7e-124
Identity = 209/309 (67.64%), Postives = 260/309 (84.14%), Query Frame = 0


Query: 6   KMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPAS 65
           K RVLVVG TGY+G+RIV+A L +GH+TYV+QRPEIGL+I+K+QL LSFKK GA +V  S
Sbjct: 9   KTRVLVVGATGYIGKRIVRACLAEGHETYVLQRPEIGLEIEKVQLFLSFKKLGARIVEGS 68

Query: 66  FSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPA 125
           FSD QSLV AVK VDVV+SAMSGVHFRSHN+L+QLKLVEAI+ AGNVKRFLPSE+G+DP 
Sbjct: 69  FSDHQSLVSAVKLVDVVVSAMSGVHFRSHNILVQLKLVEAIKEAGNVKRFLPSEFGMDPP 128

Query: 126 RMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVT 185
           RMG AL PGR +FD+KM +R+AIE A IP+TYV   CFA YFAG+LSQM TL+PP  +V 
Sbjct: 129 RMGHALPPGRETFDQKMEVRQAIEAAGIPYTYVVGACFAAYFAGNLSQMVTLLPPKEKVN 188

Query: 186 LYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQ 245
           +YGDG+VKV++ DEDDIA Y  KT +DPRTLNKTV I+PP+N+LTQ EL++ WEKL+GK+
Sbjct: 189 IYGDGNVKVVFADEDDIAKYTAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKE 248

Query: 246 LQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEGEEASKLYPEVNYTR 305
           L+K  ++AQDFLA ++  ++  + G+GHFYHIF+EGCLT+ E+GE EEAS LYP+V Y R
Sbjct: 249 LEKTNIAAQDFLANIEQMEIPHQAGIGHFYHIFYEGCLTDHEVGEDEEASSLYPDVKYKR 308

Query: 306 MDDYLKIFL 315
           MDDYL++FL
Sbjct: 309 MDDYLRMFL 317

[Close]

BLAST of Tan0013435 vs. TAIR 10
Match: AT4G13660.1 (pinoresinol reductase 2 )

HSP 1 Score: 421.8 bits (1083), Expect = 4.9e-118
Identity = 202/309 (65.37%), Postives = 256/309 (82.85%), Query Frame = 0


Query: 6   KMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPAS 65
           K RVLVVGGTG LGRRIV A L +GH+TYV+QRPEIG+DI+K+QLLLSFK+ GA LV  S
Sbjct: 9   KTRVLVVGGTGSLGRRIVSACLAEGHETYVLQRPEIGVDIEKVQLLLSFKRLGAHLVEGS 68

Query: 66  FSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPA 125
           FSD QSLV AVK+VDVV+SAMSGVHFR+HN+ +QLKLV AI+ AGNVKRFLPSE+G+DP+
Sbjct: 69  FSDHQSLVSAVKQVDVVVSAMSGVHFRTHNIPVQLKLVAAIKEAGNVKRFLPSEFGMDPS 128

Query: 126 RMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFTLVPPTHQVT 185
           RMG A+ PG  +FD+KM IR AI+ A I  TY+   CFA YF G+LSQM TL PP ++V 
Sbjct: 129 RMGHAMPPGSETFDQKMEIRNAIKAAGISHTYLVGACFAAYFGGNLSQMGTLFPPKNKVD 188

Query: 186 LYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSGKQ 245
           +YGDG+VKV++VDEDD+A Y  KT +DPRTLNKTVY++P +NILTQ EL++ WEKL+ K+
Sbjct: 189 IYGDGNVKVVFVDEDDMAKYTAKTLNDPRTLNKTVYVRPTDNILTQMELVQIWEKLTEKE 248

Query: 246 LQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIGEGEEASKLYPEVNYTR 305
           L+K  VS  DFLA ++D +++ + G+GHFYHI++EGCLT+ E+G+ EEA+KLYP+V Y R
Sbjct: 249 LEKTYVSGNDFLADIEDKEISHQAGLGHFYHIYYEGCLTDHEVGDDEEATKLYPDVKYKR 308

Query: 306 MDDYLKIFL 315
           MD+YLKIF+
Sbjct: 309 MDEYLKIFV 317

[Close]

BLAST of Tan0013435 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.0e-62
Identity = 127/312 (40.71%), Postives = 195/312 (62.50%), Query Frame = 0


Query: 6   KMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPAS 65
           K ++LV+GGTG++G+ I++AS+  GH T  + R     D +K + + +FK  G  L+   
Sbjct: 5   KSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGD 64

Query: 66  FSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPA 125
            +D +SLV A+K+ DVVIS +      S  +L Q K++ AI+ AGNVKRFLPSE+G+D  
Sbjct: 65  LNDHESLVKAIKQADVVISTVG-----SMQILDQTKIISAIKEAGNVKRFLPSEFGMDVD 124

Query: 126 RMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQM--FTLVPPTHQ 185
           +   A+EP + +F  K++ R+ IE   IP+TY+  N FAGY+  +L Q+      PP  +
Sbjct: 125 K-SSAVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDK 184

Query: 186 VTLYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSG 245
           V ++GDG+VK +   E+DIA+Y +K  DDPRTLNKT+YI PP N L+  E++  WEK  G
Sbjct: 185 VKIFGDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIG 244

Query: 246 KQLQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEI--GEGEEASKLYPEV 305
           K ++K  +S +     +++  + + V +   + +F +G  TNF I    G EAS+LYP++
Sbjct: 245 KSVEKIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDI 304

Query: 306 NYTRMDDYLKIF 314
            YT +D+YL  F
Sbjct: 305 KYTSIDEYLSYF 310

[Close]

BLAST of Tan0013435 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.1e-61
Identity = 128/311 (41.16%), Postives = 187/311 (60.13%), Query Frame = 0


Query: 6   KMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPAS 65
           K ++LV+GGTGY+G+ +V+AS   GH T+ + R     D  K + + SFK  G  ++   
Sbjct: 5   KSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGD 64

Query: 66  FSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPA 125
            +D +SLV A+K+VDVVIS +      S  +L Q K++ AI+ AGNVKRFLPSE+G+D  
Sbjct: 65  LNDHESLVKAIKQVDVVISTVG-----SMQILDQTKIISAIKEAGNVKRFLPSEFGVDVD 124

Query: 126 RMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQM--FTLVPPTHQ 185
           R   A+EP + +F  K++IR+ IE   IP+TY    CF GY+  +L Q       PP  +
Sbjct: 125 RT-SAVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDK 184

Query: 186 VTLYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENILTQRELIEKWEKLSG 245
           VT+ GDG+ K +   E+DIA+Y +K  DDPRTLNK +YIKP  N L+  E++  WEK  G
Sbjct: 185 VTILGDGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIG 244

Query: 246 KQLQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLT-NFEIGEGEEASKLYPEVN 305
           K L+K  +  +  L  +++  +   V +   + +F  G    + E   G EAS+LYP+V 
Sbjct: 245 KSLEKTHLPEEQLLKSIQESPIPINVVLSINHAVFVNGDTNISIEPSFGVEASELYPDVK 304

Query: 306 YTRMDDYLKIF 314
           YT +D+YL  F
Sbjct: 305 YTSVDEYLSYF 309

[Close]

BLAST of Tan0013435 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 229.9 bits (585), Expect = 2.7e-60
Identity = 134/326 (41.10%), Postives = 194/326 (59.51%), Query Frame = 0


Query: 6   KMRVLVVGGTGYLGRRIVKASLLQGHQTYVVQRPEIGLDIDKLQLLLSFKKQGALLVPAS 65
           K ++LV+GGTGY+G  IV+ S   G+ T+ + R     D  K + + SFK  G  ++   
Sbjct: 5   KSKILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGD 64

Query: 66  FSDLQSLVDAVKRVDVVISAMSGVHFRSHNLLMQLKLVEAIQVAGNVKRFLPSEYGIDPA 125
            +D +SLV A+K+VDVVIS +         +  Q K++ AI+ AGNVKRFLP+E+GID  
Sbjct: 65  LNDHESLVKAIKQVDVVISTIG-----HKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVE 124

Query: 126 RMGDALEPGRVSFDEKMRIRKAIEEANIPFTYVSANCFAGYFAGSLSQMFT--------- 185
           R   A+EP +  F  K++IR+AIE   IP+TYV +NC AG++  +L Q  +         
Sbjct: 125 RT-SAVEPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDK 184

Query: 186 -------LVPPTHQVTLYGDGDVKVIYVDEDDIASYVVKTTDDPRTLNKTVYIKPPENIL 245
                   VPP  +VT+ GDG+ KV+   E+D+A+Y++K  DD RTLNKT+YI PP NIL
Sbjct: 185 AIIFGDKNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNIL 244

Query: 246 TQRELIEKWEKLSGKQLQKNTVSAQDFLAKMKDGDLAWKVGVGHFYHIFFEGCLTNFEIG 305
           +  E++  WEK  GK L+K  +S +  L  ++     +K  + H   +F +G  T+F I 
Sbjct: 245 SMNEMVTLWEKKIGKSLEKTHISEEQILKSIQVPIDVFK-SINH--AVFVKGDQTSFTIE 304

Query: 306 E--GEEASKLYPEVNYTRMDDYLKIF 314
              GEEAS LYP+V YT +D+YL  F
Sbjct: 305 PWFGEEASVLYPDVKYTSIDEYLSQF 321

The following BLAST results are available for this feature:

BLAST of Tan0013435 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52581	1.4e-138	76.85	Isoflavone reductase homolog OS=Lupinus albus OX=3870 PE=2 SV=1	[more]
Q9FVQ6	3.8e-123	67.64	Pinoresinol reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PRR1 PE=1 SV=1	[more]
A3R052	3.2e-122	69.68	Bifunctional pinoresinol-lariciresinol reductase OS=Linum perenne OX=35941 GN=PL...	[more]
P0DKC8	2.5e-119	67.41	Bifunctional pinoresinol-lariciresinol reductase OS=Linum usitatissimum OX=4006 ...	[more]
Q4R0H9	4.3e-119	67.41	Bifunctional pinoresinol-lariciresinol reductase 1 OS=Linum usitatissimum OX=400...	[more]

BLAST of Tan0013435 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022967298.1	1.6e-150	87.06	isoflavone reductase homolog [Cucurbita maxima]	[more]
XP_023511514.1	4.5e-150	87.06	isoflavone reductase homolog [Cucurbita pepo subsp. pepo]	[more]
XP_038887505.1	4.5e-150	86.41	isoflavone reductase homolog [Benincasa hispida]	[more]
KAG6571626.1	5.9e-150	86.73	hypothetical protein SDJN03_28354, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022159309.1	9.5e-148	84.24	isoflavone reductase homolog [Momordica charantia]	[more]

BLAST of Tan0013435 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HUP1	7.6e-151	87.06	isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466861 PE=4 SV...	[more]
A0A6J1E225	4.6e-148	84.24	isoflavone reductase homolog OS=Momordica charantia OX=3673 GN=LOC111025718 PE=4...	[more]
A0A6J1HLT4	4.6e-148	85.76	isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464122 PE=4 ...	[more]
A0A6J1HJH3	1.7e-147	83.44	isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464118 PE=4 ...	[more]
A0A6J1HWD5	2.3e-147	83.77	isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466862 PE=4 SV...	[more]

BLAST of Tan0013435 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32100.1	2.7e-124	67.64	pinoresinol reductase 1	[more]
AT4G13660.1	4.9e-118	65.37	pinoresinol reductase 2	[more]
AT1G75290.1	1.0e-62	40.71	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G75280.1	1.1e-61	41.16	NmrA-like negative transcriptional regulator family protein	[more]
AT1G75300.1	2.7e-60	41.10	NmrA-like negative transcriptional regulator family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008030	NmrA-like domain	PFAM	PF05368	NmrA	coord: 9..301 e-value: 1.9E-57 score: 194.5
None	No IPR available	GENE3D	3.90.25.10		coord: 161..310 e-value: 1.9E-119 score: 400.2
None	No IPR available	GENE3D	3.40.50.720		coord: 8..299 e-value: 1.9E-119 score: 400.2
None	No IPR available	PANTHER	PTHR43349:SF45	PINORESINOL-LARICIRESINOL REDUCTASE-LIKE PROTEIN	coord: 5..313
None	No IPR available	PANTHER	PTHR43349	PINORESINOL REDUCTASE-RELATED	coord: 5..313
None	No IPR available	CDD	cd05259	PCBER_SDR_a	coord: 8..309 e-value: 9.80389E-71 score: 219.097
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 6..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0013435.1	Tan0013435.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009807	lignan biosynthetic process
molecular_function	GO:0016491	oxidoreductase activity